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1.
M L Hansmann E Kaiserling 《Virchows Archiv. B, Cell pathology including molecular pathology》1982,39(3):323-332
Lacunar cells, which are characteristic of the nodular sclerosis type of Hodgkin's disease, were investigated by light and electron microscopy and by enzyme cytochemical and immunohistochemical methods. Characteristic ultrastructural features of the lacunar cells were its size, its multilobated nucleus, and the pale cytoplasm containing only a few organelles. These features distinguish the lacunar cell from typical Sternberg-Reed and Hodgkin cells. Enzyme cytochemically, lacunar cells were weakly positive for acid phosphatase and non-specific esterase. the reaction product was distributed either diffusely or more focally in the cytoplasm. By immunostaining, kappa, lambda, and IgG could be detected in some lacunar cells. The immunostaining pattern was bitypic, which might have resulted from non-specific uptake. All the results of the present study indicate that lacunar cells are non-lymphoid cells. When lacunar cells were compared with cells of normal lymphoid tissue, their ultrastructure was found to be very similar to that of interdigitating reticulum cells. Both cell types showed a bizzarrely shaped nucleus and an electron-transparent cytoplasm with only some vesicles and tubules. Furthermore, lacunar cells and interdigitating reticulum cells exhibited a similar reaction pattern of acid phosphatase and non-specific esterase. Thus, from a cytologic and enzyme cytochemical point of view, a direct relationship between the two cell types is very likely. 相似文献
2.
Dr. E. W. A. Kamperdijk M. L. Kapsenberg M. van den Berg E. C. M. Hoefsmit 《Cell and tissue research》1985,242(3):469-474
Summary Non-lymphoid dendritic cells were isolated from normal and paratyphoid vaccine-stimulated lymph nodes draining the rat skin. They were studied using enzymecytochemical, immunocytochemical and electron-microscopical methods. These cells had an irregular outline and an eccentrically situated nucleus. All showed acid phosphatase activity in a central area and expressed Ia antigen on the plasma membrane. Birbeck granules were exclusively present in dendritic cells isolated from lymph nodes in the induction phase of the immune response. This observation concurs with the presence of Birbeck granules in interdigitating cells in situ during the same period of the immune response. It is concluded that the dendritic cells are the in-vitro equivalents of the non-actively phagocytizing population of interdigitating cells. 相似文献
3.
Dr. Tatsuo Ushiki Toshihiko Iwanaga Toru Masuda Yasuo Takahashi Tsuneo Fujita 《Cell and tissue research》1984,235(3):509-514
Summary The present study deals with the localization and ultrastructure of S-100-immunoreactive cells in the human thymus. These immunoreactive cells are distributed mainly in the medulla with some scattered elements in the cortex. Electron-microscopic observation revealed that the cells are characterized by an irregularly shaped nucleus, tubulovesicular structures in the cytoplasm and characteristic interdigitations of the plasma membrane. The cells often embrace lymphocytes with their branched processes. On the basis of these morphological features, the immunostained elements were identified as interdigitating cells (IDCs). The immunocytochemistry for S-100 visualizes the precise distribution and extension of the IDCs under the light microscope and indicates that the IDCs form no structural networks such as those established by the thymic epithelial cells. Since the IDCs in human lymph nodes have also been reported to contain S-100-like immunoreactivity, S-100 protein can be regarded as a useful marker for identifying the IDCs in the human thymus and other lymphoid organs. 相似文献
4.
Gerda J. van der Brugge-Gamelkoorn Marja B. van de Ende T. Sminia 《Cell and tissue research》1985,239(1):177-182
Immunohistochemical, enzyme-histochemical and electron-microscopical methods were used to study non-lymphoid cells of control and stimulated rat bronchus associated lymphoid tissue (BALT) in situ and in suspensions. Particular attention was paid to the so-called antigen-handling cells, i.e., the interdigitating cells (IDC), which are situated in the T-cell areas, the follicular dendritic cells (FDC), which appear to be restricted to germinal centers, and macrophages, present both in T-cell and B-cell areas. The interdigitating cells were distinguished by being Ia-positive and by the presence of acid phosphatase and non-specific esterase activity in an area near the nucleus. Follicular dendritic cells could be observed in situ by using a monoclonal antibody and by the in vitro trapping of HRP-anti-HRP complexes. Several types of macrophages were found. At the electron-microscopical level no well-developed IDC and FDC could be detected in control BALT. However, in BALT of lipopolysaccharide-stimulated and mycoplasma-infected rats, well-developed IDC and FDC were found. It can be concluded that IDC's and FDC's can be found in BALT. 相似文献
5.
Summary We have previously shown that the interaction of thymocytes with thymic accessory cells (macrophages and/or interdigitating cells) is one of the factors required for thymocyte activation. Precursors of both thymic accessory cell and thymocytes are included in the CD4- CD8- Mac-1- Ia- subpopulation, and their respective maturation and/or activation may be modulated by granulocyte-macrophage colony-stimulating factor, interleukin 1 and interleukin 2. When CD4- CD8- thymic cells are activated with granulocyte-macrophage colony-stimulating factor plus interleukin 2, both macrophages and interdigitating-like cells are present, as shown by electron microscopy. When activated with interleukin 1 plus interleukin 2, the interdigitating-like cells is the only accessory cell present. In both culture conditions, large clusters are formed between interdigitating cells and lymphoid cells. These results have led us to propose two-step signals for thymocyte proliferation: first, the maturation of macrophages under granulocyte-macrophage colony-stimulating factor control and the production of interleukin 1, and secondly, the maturation of interdigitating cells under interleukin 1 control, their clustering with thymocytes which are then activated.Abbreviations CFU-S
colony-forming units in the spleen
- CSF
colony-stimulating factor
- DC
dendritic cells
- DN
double negative cells (CD4- CD8-)
- EC
epithelial cells
- GM-CFC
granulocyte/macrophage colony-forming cells
- GM-CSF
granulocytemacrophage CSF
- IDC
interdigitating cell
- IL-1
interleukin 1
- IL-2
interleukin 2
- MØ
macrophage
- P-TR
phagocytic cell of the thymic reticulum 相似文献
6.
Summary Interdigitating cells (IDC) in the thymus of the spotless starling, Sturnus unicolor, were examined by electron microscopy. They occur principally in the thymic medulla and corticomedullary border. They possess an irregular nucleus and a perinuclear area of cytoplasm, containing most of the membranous organelles, surrounded by a peripheral electron-lucent zone. Clusters of smooth Golgi vesicles and complicated labyrinthine membrane-membrane contacts are the most characteristic cytological features. Birbeck granules are absent. Lymphocytes, plasma cells and even myoid cells can be found embedded in the cytoplasm. Immature elements, intermediate between epithelial-reticular cells and interdigitating cells, are tentatively identified as prointerdigitating cells. The functional significance of IDCs, and their phylogenetic significance in the vertebrate immune system, is discussed. 相似文献
7.
A. Wiersbowsky V. Grouls Prof. Dr. B. Helpap G. Klingmüller 《Cell and tissue research》1982,223(2):335-348
Summary To obtain more information concerning the origin of interdigitating cells, the postnatal development and morphology of the periarteriolar lymphatic sheath in splenic white pulp of rats was investigated by light- and electron-microscopy. Special attention was paid to the ontogeny of interdigitating cells. The spleens of the animals were studied in the age range from 1 h to 28 days after birth.The splenic white pulp of neonatal rats consists only of a few reticuloblasts, which are concentrically arranged around central arterioles. After 21 h an increase in promonocytes and monocytes was noted. Between the fifth and seventh postnatal day monocytogenic cells with a light and almost translucent cytoplasm appear, which display long cytoplasmic projections between the adjacent cells. Neighbouring lymphocytes often insert finger-like processes into the invaginated cellular membrane of these transitional forms. This intimate cellular contact is supported by zonulae occludentes. These cells represent transitional forms between monocytes and interdigitating cells.From seven days of age onwards typical interdigitating cells were present as in adult animals. After the differentiation into an inner and outer periarteriolar lymphatic sheath, the T-cell-dependent area of splenic white pulp has attained its adult appearance and further changes are not to be expected.On the basis of these findings, it is highly probable that interdigitating cells develop via transformation of monocytes.This investigation was supported by the Deutsche Forschungsgemeinschaft (*He 537) 相似文献
8.
R C Murphy 《Journal of morphology》1971,133(1):1-15
The pineal organ of the bluefin tuna was studied using light and electron microscopy. The pineal, an evagination in the diencephalic pallium, consists of a distal expanded end-organ and a stalk. A pineal nerve connects this end-organ to the habenular area of the diencephalon. The tissues above the pineal are modified for light transmission. Supporting cells are the most numerous type seen in the pineal tissue. The cytoplasm often contains glycogen, while the golgi apparatus is seldom seen. The plasma membrane is a complex of folded membranes interdigitating with adjacent cells. The sensory cells are characterized by a basal nucleus, a constricted neck region, and an ellipsoid region that is capped distally by lamellae. Sensory cells have the basic appearance of retinal photoreceptors. The lamellae are more disorganized than those of retinal rods, and some lamellae appear to be discharged from the cell. This study indicates that the pineal may function to deliver photoperiodic stimuli to the central nervous system, through the transmission of nerve impulses. 相似文献
9.
R Alvarez 《Journal of morphology》1990,204(3):305-312
In the thymus of Rana perezi, as in other anuran amphibians, there exist two different portions, cortex and medulla. In both sections epithelio-reticular cells are observed as are lymphocytes, macrophages, and granulocytes. In addition, the medulla shows cysts and secretory, myoid, and hypertrophied epithelio-reticular cells. In the cortex, plasma cells and interdigitating cells were also observed. Interdigitating cells make contact with lymphocytes. This finding provides morphological support for the hypothesized role of interdigitating cells in antigen presentation. 相似文献
10.
C R Braekevelt 《Histology and histopathology》1992,7(3):471-477
The retinal pigment epithelium (RPE) of the red-backed salamander (Plethodon cinerus) consists of a single layer of large squamous shaped cells. The RPE cells are but minimally infolded basally (sclerally) but show many large apical (vitreal) processes interdigitating with the rod outer segments. These epithelial cells are joined laterally by prominent tight junctions located in the mid region of the cells. Internally smooth endoplasmic reticulum is very plentiful while rough endoplasmic reticulum is not. Polysomes, small dense mitochondria and small round to oval melanosomes are plentiful. Golgi zones and lysosome-like bodies are also present as are phagosomes of outer segment material and myeloid bodies. The RPE cell nucleus is large and vesicular. It is felt that the melanosomes undergo retinomotor movements but as only light-adapted specimens were examined it is not known how extensive are these movements. Bruch's membrane or complexus basalis shows the typical pentalaminate structure noted for most vertebrates. The choriocapillaris is a single layer of large anastomosing capillaries which are minimally fenestrated facing Bruch's membrane. 相似文献
11.
Monoclonal antibody against histiocytosis X cells (HXCs) was established. The antigen was the cell membrane of HXCs from the submandibular lesion of a 63-year-old man who had been diagnosed as an adult type of histiocytosis X (HX) and whose HXCs had numerous Birbeck granules (BGs). The obtained monoclonal antibody, named MI1, reacted with the antigenic cell membrane of HXC. Immunoblotting showed that MI1 bound to the cell membrane of 28500 mw. MI1 also reacted with interdigitating reticulum cells (IDCs) in the tonsil and Langerhans cells (LCs) in the epidermis. MI1 reacted with the BGs which connected to the cell membrane, but not with those located near the nucleus. 相似文献
12.
Summary Ultrastructural studies of renal papillae of New Zealand brown rabbits under different states of water balance indicate no morphological variation between control, antidiuretic and diuretic animals; the only exception being a decrease in the amount of glycogen in the collecting duct cells in the antidiuretic state and an increase in the diuretic.The light cells of the collecting ducts have a low electron density and show a paucity of organelles. These comprise mitochondria, Golgi apparatus, multivesicular bodies, sparse endoplasmic reticulum and free ribosomes. The centrally-placed, spherical nucleus demonstrates large numbers of nuclear pores. The lateral surfaces and bases of the cells have considerable infoldings which may have functional significance.The attenuated endothelial cells of the vasa recta are punctuated by fenestrations which are most frequently crossed by membrane. The cells contain micropinocytotic and pinocytotic vesicles.The loops of Henle in the papilla are lined by squamous cells which are extended longitudinally in the form of interdigitating processes. The bases of the cells of most loops are scalloped.The interstitial cells are embedded in an amorphous matrix containing occasional collagen fibres and strands of fibrillar material. The cells are irregular in outline and have moderately developed endoplasmic reticulum and Golgi apparatuses.Tight junctions between the cells of all collecting ducts, loops of Henle and vasa recta are a constant finding. All these tubular elements are surrounded by a prominent basement membrane; that associated with the loops of Henle tends to be multiplied, particularly at scalloped regions. The membrane associated with the vasa recta is single except at regions where it projects across the interstitium to the membranes of the collecting ducts and loops of Henle.The functional implications of these findings are discussed. 相似文献
13.
Drs. P. Eikelenboom 《Cell and tissue research》1978,195(3):445-460
Summary Non-lymphoid cells (marginal metallophils, follicular immunecomplex-retaining cells, interdigitating cells), which are present in certain areas of the white pulp in the mouse spleen were characterized by means of (immuno)enzyme histochemical techniques, carbon uptake and experiments with lethal X-irradiation. Marginal metallophils are clearly present at the inner border of the marginal zone and show a very strong, E-600 sensitive, non-specific esterase (NSE) activity. Follicular immune-complex-retaining cells show a weak and diffuse NSE activity and no carbon uptake as shown by the combined application of an immunohistoperoxidase technique (for the demonstration of immune complexes), enzyme histochemistry (for NSE activity) and carbon uptake (for phagocytosis). Interdigitating cells show a distinct focus of NSE activity in the cytoplasm, weak carbon uptake and high radiation sensitivity. Demonstration of NSE activity is useful for the identification of the different non-lymphoid cells in the white pulp of the mouse spleen. It is suggested that the in vitro observed dendritic cells of Steinman and Cohn (1973) belong to the mononuclear phagocyte system, as transitional cells are encountered with cytological features of both dendritic cells and macrophages. These in vitro dendritic cells (or a portion of them) are probably similar to the interdigitating cells.Abbreviations HRP
horseradish peroxidase
- IDC
interdigitating cells
- PALS
periarteriolar lymphocytic sheath
- NSE
non-specific esterase 相似文献
14.
Fine structures of normal and horseradish peroxidase (HRP)-stimulated tonsil-like lymphoid organs of the laboratory suncus (Suncus murinus) were examined by scanning, transmission and immunoelectron microscopies. The normal organs appear as a pair of small oval protrusions at the upper lateral sites of the fauces, and consist of a single lymph nodule with a germinal center and a crypt-like epithelium with prominent lymphoid cell infiltration. Postcapillary venules (PCV) and efferent lymphatics are associated within the marginal region of the nodule and separated from the neighboring pharyngeal tissues. Numerous lympho-plasma cells, interdigitating cells and reticulum cells occurred within the lymphoid parenchyma, as well as in the intraepithelial infiltrating cell populations. In the HRP-stimulated animals, the anti-HRP antibodies producing lympho-plasma cells were often seen in the parenchyma and epithelia; however, similar HRP-antibody-positive lymphocytes were rarely detected in the PCV lumina. In addition, some HRP antibody bearing interdigitating cells were also identified in the same parenchyma. These data indicate that the suncus' tonsil-like organs have a positive immune function to oral antigens, together with the suncus' systemic immune system and it is hypothetically presumed that the organ may correspond to a homologous organ of the human palatine tonsil in comparative anatomy. 相似文献
15.
J. Leceta A. Villena B. Razquin J. Fonfria Dr. A. Zapata 《Cell and tissue research》1984,238(2):381-385
Summary Interdigitating cells are non-lymphoid elements in the thymus and peripheral, secondary lymphoid organs of higher vertebrates. Their origin and functional significance are a matter of controversy. In the present investigation we analyze, for the first time, the nature of presumptive interdigitating cells of the thymus of an ectothermic vertebrate, the turtle Mauremys caspica. This model is specially useful because of the seasonal variations that affect the reptilian lymphoid organs. Immature pro-interdigitating cells and phagocytosing mature interdigitating cells are described with special emphasis on their ultrastructural characteristics and possible relationships with monocytes and macrophages. 相似文献
16.
E Kaiserling H Wolburg 《Virchows Archiv. B, Cell pathology including molecular pathology》1988,54(4):221-231
Lymph nodes with extensive leukemic infiltration from three patients with the Sézary syndrome were examined in ultrathin sections and in freeze-fracture replicas. Sézary cells (SC) and interdigitating reticulum cells (IDC) were the predominant cell types in the lymph nodes. Both were closely connected with each other by apparently interdigitating cytoplasmic processes. The projections between these cells were, in the main, processes from the IDC. In freeze-fracture replicas these cellular processes did not appear as interdigitations but were more bubble-like, and for this reason these cells are imprecisely described by the term "interdigitating." The SC were seen to possess only short cytoplasmic processes. The frequent polar grouping of cell organelles in SC in the region of the contact zone with IDC and the high organelle content of IDC ('activated IDC') could be the morphologic expression of intense interaction between IDC and SC. IDC displayed three features in freeze-fracture which are not specific to the Sézary syndrome, but should be applicable to IDC in general: (1) they exhibited an approximately equal density of intramembrane particles in both the E-face and the P-face, (2) some of the intramembrane particles in the P-face were assembled in clusters and (3) the surface showed bubble-like formations of the cytoplasmic processes. On the basis of these properties it was possible to distinguish IDC from macrophages and lymphocytes in freeze-fracture replicas. 相似文献
17.
D N Menton 《The American journal of anatomy》1976,145(1):1-22
It has recently been discovered that the stratum corneum and superficial living epidermis of mammalian skin are organized into neat vertical columns of interdigitating cells. The mechanism for this organization has not yet been determined. This study shows that the stacked organization is not unique to the epidermis but also occurs in cork cambium and the pith of woody plant stems. The structural and spatial organization of the stacked cells in these tissues were compared and found to approximate closely the shape of Kelvin's minimum-surface polygon, the tetrakaidecahedron. Paper models of flattened tetrakaidecahedra were constructed and found to stack columns of interdigitating units which are consistent with all of the structural details seen in stacked cells observed under the light and the scanning electron microscopes. Indeed, only polygons of this type are capable of aggregating without interstices in the manner of the stacked cells. A study of stacked arrays of these models has revealed a mechanism by which the epidermis might become organized in columns of stacked and interdigitating cells. The proposed mechanism assumes that cells within an array seek the smallest possible surface-to-volume ratio and, under appropriate conditions, form stacked tetrakaidecahedra. 相似文献
18.
Priv.-Doz. Dr. Brita von Gaudecker Ulrich Pfingsten Hans-Konrad Müller-Hermelink 《Cell and tissue research》1984,238(1):135-143
Summary In the present study attention was focussed on several lymphoid subpopulations and specific stationary cells of the human tonsilla palatina. They were labeled at the light- and electron-microscopic levels by means of monoclonal antibodies to cell surface antigens. Cells resembling interdigitating cells (IDC-like cells) within the crypt epithelium and the interdigitating cells in the parafollicular T-cell region express the HLA-DR antigen. This fact suggests a relationship between these two populations of cells. Both cell types were frequently found in close contact to T-helper cells labeled with Anti-Leu 3a. This fact is discussed as a confirmation of earlier suggestions that the tonsillar crypt epithelium serves as T-cell region. Cytotoxic/ suppressor-T cells (OKT 8
+) and Leu 7-positive cells do not appear to contact interdigitating cells. Anti-Leu 7 is a monoclonal antibody, that defines a differentiation antigen shown to be selectively expressed on human natural killer cells (NK-cells). With the use of the immuno-electron-microscopic labeling method it was possible to analyze the ultrastructure of this lymphoid subpopulation. Two morphologically distinguishable subtypes of Leu 7-positive cells populate different microenvironments: The Leu 7-positive large-granular lymphocyte was predominantly found in the crypt epithelium, while numerous Leu 7-positive cells located in the germinal centers had the appearance of small lymphocytes. This finding is discussed in favour of distinct phenotypes representing different stages in a differentiation pathway of the maturing NK-cell: Small Leu 7-positive lymphocytes in the germinal centers are supposed to be functionally inactive precursors, and only the Leu 7-positive large granulated lymphocytes in the crypt epithelium may represent differentiated active NK-cells. This interpretation is in agreement with the observation that the tonsilla palatina, in spite of containing numerous Leu 7-positive cells, shows only low NK-activity against tumor cells.Glossary of Abbreviations used in this Paper DAB
diamino-benzidine
- DMSO
dimethyl sulfoxide
- HLA
human leucocyte antigen
- HLA-DR
human leucocyte antigen, D-region related
- Ia-antigen
immune-associated antigen of the MHC
- IDC
interdigitating cell
- IDC-like cell
cell that resembles an interdigitating cell
- LGL
large granular lymphocyte
- MHC
major histocompatibility gene complex
- NK-cell
natural killer cell
- PBS
phosphate-buffered saline 相似文献
19.
Summary Seven thymuses from children between 1 and 12 years were examined by electron microscopy. Biopsies had been taken during surgical correction of congenital heart defects.In all cases we found interdigitating reticulum cells (IRC) in the medulla and inner cortex. These cells resembled the IRC which have been described previously in the thymus-dependent regions of the spleen and lymph node. They were characterized by an irregularly shaped nucleus, narrow cisterns of rough endoplasmic reticulum, and widespread interdigitation and invagination of the cell membrane. The surfaces of the IRC were in close contact with those of small lymphocytes, sometimes polysomal lymphatic cells, epithelial cells, and occasionally with those of lymphatic cells containing ergastoplasm.The IRC is apparently a specific cell of thymus-dependent regions. It may be that the IRC in the thymus, lymph node, and spleen contribute to the microenvironment needed for the differentiation of T-cells.Supported by the Deutsche Forschungsgemeinschaft, SFB 111/CII and III.—We wish to thank Miss M. Neubert and Mrs. R. Köpke for their technical assistance and Mrs. M. Soehring for her help with the translation. 相似文献
20.
大鼠脾白髓交错突细胞和巨噬细胞的形态计量分析 总被引:3,自引:0,他引:3
本文应用形态计量学方法对脾白髓内S-100蛋白阳性交错突细胞和lysozyme阳性巨噬细胞的一些形态计量参数进行比较分析,结果如下:两种细胞相比,交错突细胞和巨噬细胞的平均面积无显著差异,而平均周长和平均形态因子在交错突细胞明显大于巨噬细胞(P<0.01);在单位面积脾白髓内,交错突细胞的面数密度和面密度明显小于巨噬细胞的相应数值(P<0.01),提示这两种细胞在形态计量方面也有明显区分。鉴于这些数值与其细胞的形态和数量密切相关,并随细胞的变化而产生相应的改变,因而是有意义的形态计量参数。 相似文献