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巨噬细胞免疫调变信号:Raf-1,MAPK p44,MAPK p42和p38 MAPK的研究 总被引:2,自引:0,他引:2
为了了解巨噬细胞免疫调变机理,我们应用LPS和PMA处理小鼠抑制性巨噬细胞,观察到Ras下游信号分子Raf-1,分裂原激活蛋白激酶MAPK p44,MAPK p42和p38 MAPK均被活化,发现forskolin能增强p38 MAPK的活性,进一步提示PKC和PKA途径增强了p38 MAPK的磷酸化效应,为我们了解LPS如何激活p38 MAPK信号通路提供了一个新的机会。 相似文献
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细胞表面受体到核的信号通路是现代生物学研究的主题之一。细胞外各种刺激通过和膜受体偶联的G蛋白和酪氨酸激酶介导了一系列丝氨酸/苏氨酸激酶介导的级联反应,即分裂原激活蛋白激酶(MAPK)级联反应。MAPK级联反应把细胞外信号传递到核,并汇总了各种信号通路来的传息,因此研究细胞内MAPK的信号通路是十分重要的.本文简要介绍了ERK,JNK和p38三种MAPK途径,着重叙述了p38MAPK信号途径的性质和功能以及在免疫细胞中的作用和某些疾病的临床关系。 相似文献
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MAPK和脱落酸信号传导 总被引:3,自引:0,他引:3
ABA在调节种子发育、参与对逆境胁迫的反应中发挥重要作用,使其成为当今研究热点,特别是对ABA与MAPK相互关系的研究。本文就ABA生物学功能及其与MAPK的关系、ABA信号传导级联分子组成、ABA信号传导中的磷酸化、MAPK与ABA信号传导等方面的最新进展予以综述,以对ABA所发挥重要作用的分子机制有深入的认识。 相似文献
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Roberta R. Ruela de Sousa Karla Cristiana Souza Queiroz Ana Carolina Santos Souza Sonia A. Gurgueira Amanda Cavallari Augusto Marcio André Miranda 《Journal of enzyme inhibition and medicinal chemistry》2013,28(4):439-444
Flavonoids, polyphenolic phytochemicals, are ubiquitous in plants and are commonly present in the human diet. They may exert diverse beneficial effects, including antioxidant and anticarcinogenic activities. The present study was designed to evaluate three biomolecules that play important roles in the apoptotic process: mitogen-activated protein kinases, protein phosphatases and NFκB, using HL60 cells treated with fisetin as an experimental model. Our results demonstrated that cells treated with fisetin presented high expression of NFκB, activation of MAPK p38 and an increase of phosphoprotein levels; inhibition of enzymes involved in redox status maintenance were also observed. Our findings reinforce the hypothesis that fisetin is likely to exert beneficial and/or toxic actions on cells not through its potential as antioxidant but rather through its modulation of protein kinase and phosphatase signaling cascades. Additionally, our results also indicate that the cellular effects of fisetin will ultimately depend on the cell type and on the extent to which they associate with the cells, either by interactions at the membrane or by uptake into the cytosol. 相似文献
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M. Kırça N. Oğuz A. Çetin F. Uzuner 《Journal of receptor and signal transduction research》2017,37(2):167-173
Hyperuricemia and angiotensin II (Ang II) may have a pathogenetic role in the development of hypertension and atherosclerosis as well as cardiovascular disease (CVD) and its prognosis. The purpose of this study was to investigate whether uric acid can induce proliferative pathways of vascular smooth muscle cell (VSMC) that are thought to be responsible for the development of CVD. The phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), p44/42 mitogen-activated protein kinase (p44/42 MAPK) and platelet-derived growth factor receptor β (PDGFRβ) was measured by Elisa and Western blot techniques to determine the activation of proliferative pathways in primary cultured VSMCs from rat aorta. Results demonstrated that uric acid can stimulate p38 MAPK, p44/42 MAPK and PDGFRβ phosphorylation in a time- and concentration-dependent manner. Furthermore, treatment of VSMCs with the angiotensin II type I receptor (AT1R) inhibitor losartan suppressed p38 MAPK and p44/42 MAPK induction by uric acid. The stimulatory effect of uric acid on p38 MAPK was higher compared to that of Ang II. The results of this study show for the first time that uric acid-induced PDGFRβ phosphorylation plays a crucial role in the development of CVDs and that elevated uric acid levels could be a potential therapeutical target in CVD patients. 相似文献
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p38 MAPK信号传导通路 总被引:21,自引:0,他引:21
丝裂原活化蛋白激酶(mitogen-activatedporoteinkinase,MAPK)介导了生长、发育,分裂,死亡,以及细胞间的功能同步等多种细胞生理功能,在哺乳动物细胞中已发现和克隆了ERK、JNK/SAPK,ERK5/BMK1和p38/RK四个MAPK亚族,这些新的MAPK介导了物理,化学反激,细菌产物,炎性细胞因子等多种刺激引起的细胞反应,p38亚族至少包括p38(α),p38β,p 相似文献
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MAPK信号通路与脂肪细胞分化 总被引:1,自引:0,他引:1
促分裂原活化的蛋白激酶(MAPK)通路是真核细胞重要的信号转导通路,主要有ERK、p38和JNK三条途径,参与调控多种细胞应答和生理病理过程。该文重点讨论了MAPK对脂肪细胞分化的调控。其中ERK对脂肪细胞分化的调节具有多样性,随分化进程不同表现为不同的调控功能,p38和JNK也通过不同的机制对脂肪细胞分化发挥相异的调节作用。MAPK信号转导与脂肪分化的紧密联系,使其可能成为调控与脂分化密切相关的代谢疾病如肥胖、糖尿病等的一条关键通路。 相似文献
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真核生物的MAPK级联信号传递途径 总被引:15,自引:0,他引:15
MAPK级联途径在真核生物细胞的信号传递过程中起着重要的作用.MAPK级联途径由MAPK、MAPKK和MAPKKK三类酶蛋白组成.这三类蛋白质的结构非常保守,通过磷酸化作用传递各种信号.在酵母和动、植物细胞中已经发现了一系列的MAPK级联途径成员,使真核生物的信号传递途径逐渐得到阐明. 相似文献
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丝裂原活化蛋白激酶(MAPK)信号通路广泛存在于真核细胞并且高度保守,是生物体内非常重要的信号转导系统之一。胞外刺激信号通过细胞膜上的特异性受体传递给胞内MAPK信号通路,该信号通路通过磷酸化下游转录因子、调节各种酶类来调控转录水平及生化反应等,进而使细胞适应外界环境变化。Hog1 MAPK信号通路能够被胞外高渗透压胁迫等刺激激活,对细胞在高渗环境下的存活至关重要。近年来,越来越多的研究发现虽然该信号通路在真核生物中高度保守,但不同物种中的组成仍有差异,且该信号通路的功能也相对多元化。本文综述了Hog1 MAPK信号通路的组成、功能及其与其他信号通路之间的cross-talk,旨在为今后深入研究该信号通路的作用机制及其与其他信号通路间的cross-talk提供参考。 相似文献
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植物MAPK C族基因的研究进展 总被引:1,自引:0,他引:1
促分裂原活化蛋白激酶(MAPK)是一类丝氨酸/苏氨酸蛋白激酶,它包括MAPKKK、MAPKK和MAPK等3组,它们共同构成MAPK级联系统.MAPK级联系统通过参与蛋白质磷酸化作用传递和放大信号,调控下游基因的表达,最终引起植物的一系列生理反应.MAPK的基因包括A族、B族、C族和D族4类.就C族MAPK基因的种类和功能,以及近几年来对C族MAPK基因的研究方法作综述. 相似文献
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RhoA属于小G蛋白家族成员,p38MAPK属于丝/苏氨酸蛋白激酶家族成员.本文从巨噬细胞、肌肉细胞、成纤维细胞、神经元和肿瘤几个方面阐述RhoA-p38MAPK信号通路的研究进展,此信号通路在细胞骨架的改变和肿瘤的发生发展方面有望成为新的研究热点. 相似文献
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董世武 《国外医学:分子生物学分册》2003,25(1):37-41
MAPK信号转导途径是细胞外信号引起细胞核内反应的通道之一。通过三级酶促级联反应。最终磷酸化靶蛋白,激活转录因子,调节特定的基因表达,近年研究表明ERK,P38和JNK通路参与了成骨细胞分化增殖的信号转导过程,本介绍了这三种MAPK通路的基本概况。并着重阐述其在成骨细胞分化中的作用。 相似文献
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巨噬细胞免疫调变信号:Raf—1,MAPKp44,MAPKp42和p38MAPK的研究 总被引:1,自引:0,他引:1
为了了解巨噬细胞免疫调变机理,我们应用LPS和PMA处理小鼠抑制性巨噬细胞,观察到Ras下游信号分子AF-1,分裂原激活蛋白激酶MAPKp44,MAPKp42和p38MAPK均被活化,发现forskolin能增强p38MAPK的活性,进一步提示PKC和PAK途径增强了p38MAPK的磷酸化效应,为我们了解LPS如何激活p38MAPK信号通路提供了一个新的机会/ 相似文献
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Mitogen-activated protein kinase (MAPK) signaling cascades are evolutionarily conserved fundamental signal transduction pathways. A MAPK cascade consists of many distinct MAPKKK–MAPKK–MAPK modules linked to various upstream receptors and downstream targets through sequential phosphorylation and activation of the cascade components. These cascades collaborate in transmitting a variety of extracellular signals and in controlling cellular responses and processes such as growth, differentiation, cell death, hormonal signaling, and stress responses. Although MAPK proteins play central roles in signal transduction pathways, our knowledge of MAPK signaling in hormonal responses in rice has been limited to a small subset of specific upstream and downstream interacting targets. However, recent studies revealing direct MAPK and MAPKK interactions have provided the basis for elucidating interaction specificities, functional divergence, and functional modulation during hormonal responses. In this review, we highlight current insights into MAPKK–MAPK interaction patterns in rice, with emphasis on the biological significance of these interacting pairs in SA (salicylic acid), JA (jasmonic acid), ET (ethylene), and ABA (abscisic acid) responses, and discuss the challenges in understanding functional signal transduction networks mediated by these hormones. 相似文献
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MAPK/JNK信号传导通路研究进展 总被引:4,自引:0,他引:4
JNK信号途径参与如胚胎发育、免疫反应、细胞分化等许多正常的生理过程。近年来研究表明,JNK信号途径也参与许多病理过程:JNK介导心脏肥大反应,与Ⅱ型糖尿病发病有关,介导胰腺b细胞凋亡;JNK信号途径的异常活化与多种人类肿瘤的发生发展密切相关,因此JNK是一个潜在的治疗分子靶,已引起人们的关注。对其功能及作用的分子机制的深入研究,有助于我们对JNK相关疾病的了解和寻找可能的干预和治疗途径。 相似文献
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MAPK信号转导通路参与了人巨细胞病毒的致病过程。MAPK通路中的ERK和p38通路在人巨细胞病毒复制周期中起重要作用,通过磷酸化转录因子引起病毒及宿主相关基因的转录,从而调控人巨细胞病毒的复制;人巨细胞病毒的包膜糖蛋白及其他多种基因表达产物可通过不同机制以一定时序激活MAPK通路,调节自身及宿主细胞相应基因表达,以利于病毒完成其生活周期,并参与病毒的致病过程。深入研究MAPK信号转导通路与人巨细胞病毒感染的关系可为治疗该病毒感染引起的疾病提供新的治疗靶点。 相似文献