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1.
Fentichlor produces rapid and marked damage to cell membranes of Staphylococcus aureus and Escherichia coli causing leakage of 260 nm material and pentose from whole cells, and lysis of spheroplasts of E. coli. Investigations indicate that Fentichlor-induced membrane damage is related to bactericidal activity. Bacteriostatic concentrations of 10 μg/ml or less do not stimulate leakage while at higher concentrations there is a marked relationship between loss of viability and of metabolic pool material released. Drug concentrations producing 99·99% kill within 1 h correspond closely to minimum concentrations required to produce total loss of 260 nm material from the pool. Membrane damage caused by bactericidal concentrations of drug is irreversible and under conditions optimal for enzyme activity additional leakage of 260 nm material resulting from autolytic breakdown of insoluble nucleic acids also occurs. There is no evidence that Fentichlor produces precipitation of cell cytoplasmic constituents. 相似文献
2.
The antibacterial activity of four local anaesthetics (LAs), amethocaine and procaine (esters), and cinchocain and lignocaine (amides), has been studied. All four LAs inhibited cell growth but only amethocaine and cinchocain had significant effects on cell viability and on the leakage of cellular constituents. Effects on growth inhibition were reversible. Concentrations causing marked loss in viability also caused the leakage of cellular constituents. Uptake isotherms for all four LAs by E. coli are presented and an attempt made to relate derived intracellular LA levels with effects on growth inhibition. Cultures of E. coli grown in the presence of low levels of LAs effects reflecting the relative hydrophilic-lipophilic nature of the individual LAs. 相似文献
3.
The growth inhibitory and bactericidal activities of eight alkyl esters of gallic acid towards Escherichia coli NCTC 5933 have been determined. A previously suggested role for gallic acid and its esters as shikimate antimetabolites could not be substantiated. No induction of gross changes in cell morphology was observed. Bactericidal activity was accompanied only be very slight leakage of general ionic materials from the bacteria. Propyl gallate did not appear to uncouple oxidative phosphorylation from respiration as indicated by its failure to stimulate proton translocation across the cytoplasmic membrane. 相似文献
4.
The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil) 总被引:3,自引:0,他引:3
Cox SD Mann CM Markham JL Bell HC Gustafson JE Warmington JR Wyllie SG 《Journal of applied microbiology》2000,88(1):170-175
The essential oil of Melaleuca alternifolia (tea tree) exhibits broad-spectrum antimicrobial activity. Its mode of action against the Gram-negative bacterium Escherichia coli AG100, the Gram-positive bacterium Staphylococcus aureus NCTC 8325, and the yeast Candida albicans has been investigated using a range of methods. We report that exposing these organisms to minimum inhibitory and minimum bactericidal/fungicidal concentrations of tea tree oil inhibited respiration and increased the permeability of bacterial cytoplasmic and yeast plasma membranes as indicated by uptake of propidium iodide. In the case of E. coli and Staph. aureus, tea tree oil also caused potassium ion leakage. Differences in the susceptibility of the test organisms to tea tree oil were also observed and these are interpreted in terms of variations in the rate of monoterpene penetration through cell wall and cell membrane structures. The ability of tea tree oil to disrupt the permeability barrier of cell membrane structures and the accompanying loss of chemiosmotic control is the most likely source of its lethal action at minimum inhibitory levels. 相似文献
5.
Some Biological Effects of Carbamoyloxyurea, an Oxidation Product of Hydroxyurea 总被引:1,自引:0,他引:1
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Herbert S. Rosenkranz 《Journal of bacteriology》1970,102(1):20-23
Carbamoyloxyurea, an oxidation product of hydroxyurea, is bactericidal for Escherichia coli. Drug-induced killing is independent of cellular metabolism; ribonucleic acid and protein syntheses are the processes most affected, and the lethal action is accompanied by degradation of cellular deoxyribonucleic acid. In all of these effects the drug differs from hydroxyurea, a primarily bacteriostatic agent that inhibits deoxyribonucleic acid synthesis, whose lethal action ultimately depends on cellular activity. 相似文献
6.
Collagen synthesis by human glomerular cells in culture 总被引:4,自引:0,他引:4
The intracellular localization of enterotoxin in Escherichia coli AP1, a strain of porcine origin which produces high levels of heat-labile, but no heat-stable enterotoxin, has been examined. The cytoplasmic and outer membranes of this strain both contained enterotoxin activity, while the membranes isolated from a serologically related non-enterotoxigenic strain (E. coli AP2) also of porcine origin, did not show enterotoxin activity. The periplasmic fraction isolated from the enterotoxigenic strain contained considerable enterotoxin activity, but this activity was associated with outer membrane fragments present in the periplasmic fraction. Thus, of the total cellular enterotoxin activity, about 55%, 15% and 30% were present in the outer membrane, cytoplasmic membrane and the cell cytoplasm, respectively. The specific activity of enterotoxin was 20 units per mg protein in the cytoplasm and 90 and 150 units per mg protein in the cytoplasmic and outer membranes, respectively. 相似文献
7.
Binding of some polyhexamethylene biguanides to the cell envelope of Escherichia coli ATCC 8739 总被引:1,自引:0,他引:1
Absorption and antibacterial action of some polyhexamethylene biguanides upon Escherichia coli has been investigated. An amine-ended-dimer (AED) (n = 2), a polydisperse mixture sold by ICI Limited as the active ingredient of vantocil IB (PHMB) (n = 5.5), and a high molecular weight fraction of PHMB (HMW, n greater than or equal to 10) were used. Bactericidal activity was determined over a range of pH (5-9). Similarly, adsorption of drug to the cell surface, indicated by changes in electrophoretic mobility, and overall drug absorption by the cells were determined. Maximal activity occurred at pH 6 for PHMB and HMW and at pH 5 for AED. This corresponded to minimal surface adsorption and maximal distribution of drug to the underlying cytoplasm and cytoplasmic membrane. Uptake of drug corresponded to high affinity isotherms and indicated a rapid transfer of biocide into the cells following their initial interaction at the surface. 相似文献
8.
Ribonucleotide reductase is a highly regulated, rate-limiting activity in the synthesis of DNA. A previous study has shown that the Escherichia coli enzyme is inhibited by the clinically important antitumor agent cis-diamminedichloroplatinum(II) (DDP), and this has led to the hypothesis that ribonucleotide reductase is an important site of action for this chemotherapeutic agent. This hypothesis has been directly tested in this investigation. We observed that DDP inhibits the mammalian ribonucleotide reductase, with 50% inhibition occurring at 0.3 mM. Unlike the E. coli enzyme where only one of the two protein components is targeted by DDP, we observed that both of the mammalian proteins (R1 and R2) were sites for the inhibitory activity of the drug. Colony-forming experiments, enzyme activity studies, and analyses of R1 and R2 message levels in mutant cell lines containing either high levels of ribonucleotide reductase activity or exhibiting resistance to the cytotoxic effects of DDP were used to further investigate the potential role of ribonucleotide reductase in DDP cytotoxic action and drug resistance. These studies did not support a hypothesis formulated in the earlier investigation that inhibition of ribonucleotide reductase is an important component of DDP cytotoxic activity or that it is a major participant in DDP resistance mechanisms. From a biological point of view, DDP is a very active drug, and in addition to its cytotoxic effects it is capable of inducing a variety of cellular changes. Whether or not the inhibition of mammalian ribonucleotide reductase activity that we have described in this study plays a role in mediating any of these other effects remains to be determined. 相似文献
9.
Abstract Expression of bacteriophage φX174 gene E from plasmid pUH51 induced lysis of Escherichia coli . Before onset of bacterial lysis, cellular phospholipase activity was induced due to the presence of gene E product within the cells. By comparison of the lytic behaviour of phospholipase-negative E. coli strains with the corresponding wild-type strain it was found that neither the action of detergent-resistant phospholipase A nor of detergent-sensitive phospholipase were essential for the lysis-inducing properties of the gene E product. It was concluded that induction of phospholipases after expression of the φX174 gene E was a consequence of membrane perturbation caused by the integration of the gene E product into the cytoplasmic membrane of E. coli . 相似文献
10.
A A Kim E S Molchanova B N Il'iashenko 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1985,(10):25-28
The Escherichia coli K12 mutant having the increased efficiency for plasmid DNA transformation has been shown to possess the different protein composition of the outer membrane of the cellular wall, as compared with that of the wild type strain. Correlation between the level of calcium-dependent plasmid transformation and the portion of infections DNA bound with cytoplasmic membranes is demonstrated for the Escherichia coli cells mutant for outer membrane structure and ability to be transformed by plasmid DNA. 相似文献
11.
B P Goldstein R R Vidal-Plana B Cavalleri L Zerilli G Carniti L G Silvestri 《Journal of general microbiology》1977,100(2):283-298
Although the target of the antimicrobial drug 1-methyl-2-nitro-5-vinylimidazole (MEV) has been shown to be DNA (Goldstein et al., 1977) the drug was ineffective in cell-free systems because it was not activated. Both the rate of metabolic activation of MEV and its antibacterial activity were increased when bacteria were grown in limiting oxygen. Mutants of Escherichia coli which were conditionally resistant to nitroimidazoles and nitrofurans were defective in drug activation. The activities of these drugs against E. coli correlated with their rates of metabolism. The antimicrobial spectrum of the drugs appeared to be related to their reducibility by different species. 相似文献
12.
Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents 总被引:21,自引:8,他引:13
Cook, Thomas M. (Sterling-Winthrop Research Institute, Rensselaer, N.Y.), William H. Deitz, and William A. Goss. Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents. J. Bacteriol. 91:774-779. 1996.-Treatment of Escherichia coli 15TAU with nalidixic acid resulted in degradation of the nucleic acids of the cells, whereas protein was unaffected. Deoxyribonucleic acid (DNA) degradation appeared to be more extensive than ribonucleic acid degradation during periods of comparable bactericidal action. The onset of DNA degradation was evident prior to a measurable bactericidal effect. However, within the range of 2 to 20%, DNA degradation was accompanied by a decrease in viable cell numbers. Degradation of DNA to acid-soluble material occurred only under conditions permitting the bactericidal action of nalidixic acid. Arrest of the bactericidal action of nalidixic acid by the addition of dinitrophenol or chloramphenicol also inhibited DNA degradation. The acid-soluble products, which were excreted into the medium, have not been characterized completely, but probably were not phosphorylated. 相似文献
13.
Eduardo Díaz Mathildah Munthali Heinrich Lünsdorf Joachim-Volker Höltje & Kenneth N. Timmis 《Molecular microbiology》1996,19(4):667-681
The holin function Ejh of the pneumococcal bacteriophage EJ-1 has been characterized. It shows structural features similar to, and functionally complemented, the prototype member of the holin family. In Escherichia coli and Pseudomonas putida the Ejh product caused cellular death, and changes in cell morphology could be accounted for by lesions in the cytoplasmic membrane. Expression of ejh resulted in the inhibition of growth in a variety of phylogenetically distant bacterial genera, suggesting a broad spectrum of action. Concomitant expression of the ejh and ejl (encodes a lysin) genes led to lysis of E. coli and P. putida cells. Remarkably, the Ejl lysin was able to attack murein from bacteria lacking choline in their sacculi, which suggests that pneumococcal lysins have a broader substrate specificity than previously assumed. Furthermore, the Ejh holin was able to trigger activity of the major pneumococcal autolysin cloned and expressed in E. coli , and this raised new questions about the regulation of this model autolysin. A new function for holins in systems where the phage lysin is supposed to be associated with the membrane is proposed. 相似文献
14.
Bhandary S Chaki S Mukherjee S Das S Mukherjee S Chaudhuri K Dastidar SG 《Indian journal of experimental biology》2012,50(7):491-496
The antimicrobial efficacy of methylglyoxal (MG) against several gram-negative bacteria including Escherichia coli has been reported. To determine the mechanism of action of MG, molecular interactions between lipid and MG within the liposomal membrane were also investigated. Multilamellar and unilamellar vesicles were prepared from 1, 2-dipalmitoyl-snglycero-3-phosphocholine (DPPC). The effect of MG on DPPC liposomal membrane was studied by fluorescence spectroscopy and differential scanning calorimetry. The results indicate that MG interacts mainly with the DPPC head group that produces a significant increase in the fluidity of liposomal vesicles, which could be the cause of a fusion/aggregation effect in microbial cells. The agarose gel electrophoresis study with the genomic DNA extracted from E. coli ATCC 25922 revealed that addition of MG could completely degrade this DNA within 1 h, pointing out to their distinctly high degree of sensitivity towards MG. Further, the drug was able to cross the cell membranes, penetrating into the interior of the cell and interacting with DNA for demonstrating antibacterial activity of MG. 相似文献
15.
A synthetic aza-cholestane amine was found to be rapidly lethal to Saccharomyces cerevisiae. Lethal action was prevented by pretreating cells with 10m uranyl nitrate. Sublethal concentrations of the steroid prevented the uptake of glucose and alanine. This demonstrated one means by which the steroid affected cell permeability, limiting the growth of the organism. Decreases in viability were related to increases in 260 and 280 mmu absorbing materials. Uranyl protection of viability was found to be related to cellular retention of the ultraviolet-absorbing materials. The steroid, dequadin acetate, and cetyl pyridinium chloride were comparable in lethal action and in causing the leakage of cytoplasmic constituents. Loss of viability and cytoplasmic excretion were detected within minutes after cells were exposed to the steroid. The rate or degree of metabolism of glucose by resting cells did not appear to influence steroid action or the sensitivity of the cells to the steroid. 相似文献
16.
Antibacterial Activity of THAM Trisphenylguanide against Methicillin-Resistant Staphylococcus aureus
Alan J. Weaver Jr Joyce B. Shepard Royce A. Wilkinson Robert L. Watkins Sarah K. Walton Amanda R. Radke Thomas J. Wright Milat B. Awel Catherine Cooper Elizabeth Erikson Mohamed E. Labib Jovanka M. Voyich Martin Teintze 《PloS one》2014,9(5)
This study investigated the potential antibacterial activity of three series of compounds synthesized from 12 linear and branched polyamines with 2–8 amino groups, which were substituted to produce the corresponding guanides, biguanides, or phenylguanides, against Acinetobacter baumannii, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Antibacterial activity was measured for each compound by determining the minimum inhibitory concentration against the bacteria, and the toxicity towards mammalian cells was determined. The most effective compound, THAM trisphenylguanide, was studied in time-to-kill and cytoplasmic leakage assays against methicillin-resistant Staphylococcus aureus (MRSA, USA300) in comparison to chlorhexidine. Preliminary toxicity and MRSA challenge studies in mice were also conducted on this compound. THAM trisphenylguanide showed significant antibacterial activity (MIC ∼1 mg/L) and selectivity against MRSA relative to all the other bacteria examined. In time-to-kill assays it showed increased antimicrobial activity against MRSA versus chlorhexidine. It induced leakage of cytoplasmic content at concentrations that did not reduce cell viability, suggesting the mechanism of action may involve membrane disruption. Using an intraperitoneal mouse model of invasive MRSA disease, THAM trisphenylguanide reduced bacterial burden locally and in deeper tissues. This study has identified a novel guanide compound with selective microbicidal activity against Staphylococcus aureus, including a methicillin-resistant (MRSA) strain. 相似文献
17.
J.P Müllera 《FEMS microbiology letters》1999,176(1):219-227
Protein translocation through the cytoplasmic membrane of Escherichia coli involves cytosolic chaperones. The export-dedicated chaperone SecB mediates targeting of a subset of pre-proteins. In this report, synthesis of SecB in response to plasmid-mediated overexpression of pre-proteins was studied. Overexpression of SecB-dependent pre-proteins stimulated synthesis of SecB under conditions where the cellular export capacity was saturated or uncomplexed SecB was trapped. On the contrary, overexpression of SecB-independent pre-beta-lactamase reduced the promoter activity of secB. The results suggest that uncomplexed SecB can be sequestered by synthesis of SecB-dependent pre-proteins. Furthermore, these data demonstrate the distinct action of the SecB- and signal recognition particle-dependent protein targeting pathways. 相似文献
18.
Interaction of wasp venom mastoparan with biomembranes 总被引:1,自引:0,他引:1
T Katsu M Kuroko T Morikawa K Sanchika H Yamanaka S Shinoda Y Fujita 《Biochimica et biophysica acta》1990,1027(2):185-190
Mastoparan-induced changes in the K+ permeability of rat peritoneal mast cells, human erythrocytes, Staphylococcus aureus and Escherichia coli were examined. Mastoparan did not efficiently increase the K+ permeability of cells except for S. aureus. The release of membrane phospholipids was also observed from S. aureus cells in the concentration range of the permeability enhancement. Mastoparan stimulated histamine release from mast cells, independently of a small efflux of K+. Mastoparan became markedly effective to E. coli cells whose outer membrane structure was chemically disrupted beforehand, showing that the peptide can enhance the permeability of the cytoplasmic membranes of both Gram-positive and -negative bacteria. In experiments using liposomes, mastoparan increased the permeability of the liposomes composed of egg phosphatidylethanolamine and egg phosphatidylglycerol, which are the lipid constituents of the cytoplasmic membrane of E. coli cells, while it showed a weak activity to the liposomes composed of egg phosphatidylcholine and cholesterol. The latter result related closely to the fact that this peptide acted weakly on erythrocytes and mast cells in which acidic lipids constitute a minor portion. Mastoparan decreased the phase transition temperature of dipalmitoylphosphatidylglycerol liposomes, but it did not affect that of dipalmitoylphosphatidylcholine liposomes. These results indicate that mastoparan penetrated into membranes mainly containing acidic phospholipids and disrupted the membrane structure to increase the permeability. The action of the wasp venom mastoparan was compared with that of a bee venom melittin. 相似文献
19.
Mechanisms of the bactericidal action of hydrogen peroxide 总被引:2,自引:0,他引:2
I I Samo?lenko E I Vasil'eva I B Pavlova M A Tumanian 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1983,(12):30-33
The comparative study of some aspects of the bactericidal action of H2O2 on Escherichia coli, Staphylococcus aureus and Bacterium subtilis wild-type cells and their mutants with lesions in the systems of the reparation of DNA has been carried out. Hydrogen peroxide has been shown to cause disturbances in the structure and permeability of the cell wall, the cytoplasmic membrane, as well as to induce ribosomal lesions and the ruptures of bacterial DNA. The activity of the systems responsible for the reparation of lesions in the cell genome plays an important role in the resistance of bacteria to H2O2. 相似文献
20.
Localization of enterobacterial common antigen immunoreactivity in the ribosomal cytoplasm of Escherichia coli cells cryosubstituted and embedded at low temperature. 总被引:1,自引:0,他引:1
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The application of two on-section immunogold labeling techniques, the Lowicryl K4M (progressive lowering of temperature) procedure and the cryosection technique of Tokuyasu, in a previous work to study the topology of enterobacterial common antigen (ECA) biosynthesis revealed the presence of label on the outer membrane and in areas associated with the inner side of the cytoplasmic membrane. However, labeling was also observed in the ribosomal cytoplasm. The question of whether the cytoplasmic label was a result of ECA displacement during the more slowly acting aldehyde fixation or whether cytoplasmic ECA precursors are true constituents of the ribosomal cytoplasm could not be resolved from these results. In the study described here, cells of the same Escherichia coli F470 strain were reinvestigated by comparison of the progressive lowering of temperature and improved cryosubstitution-low-temperature embedment techniques. The latter procedure, applied directly to nonpretreated and noncentrifuged cells, led to superior ultrastructural preservation of the cytoplasmic organization, with little opportunity for cytoplasmic antigen displacement after the primary cryofixation step; the label distribution obtained supports the conclusion that N-acetylmannosaminuronic acid (ManNAcA)-containing ECA precursors are real constituents of the ribosomal cytoplasm. Results from tunicamycin inhibition studies of ECA biogenesis in the E. coli mutant 2465 suggested that even the ECA precursor UDP-ManNAcA alone or a chemically unidentified product(s) generated from accumulated ManNAcA residues may react with the monoclonal antibody used, leading to weak but clearly positive cytoplasmic labeling. The relatively intense labeling obtained with cells grown in the absence of the drug can be explained by the reactivity of further ManNAcA-containing ECA precursors with the monoclonal antibody used. 相似文献