Prolongation of Microbiological Air Sampling by a Monolayer on Agar Gel

The useful life of agar in aerosol samplers is greatly extended by treatment with a dilute emulsion of oxyethylene docosanol. Growth of viable organisms is unaffected.     

A Simple Method for Staining Fungal Substrate Hyphae in Agar Media

Morphogenetic processes often occur in fungal cultures in agar medium. These processes are difficult to study by light microscopy because the hyphae or other structures fail to have sufficient contrast for detailed study and photography. To overcome this difficulty, we developed a method to stain hyphae inside the agar without affecting the medium itself.     

一种简便快速筛选重组子的方法

目的：根据碱裂解法抽提质粒DNA的原理,研究应用快检缓冲液方法挑取重组子克隆,从而获得简单易行,快速方便,节省时间,提高工作效率的筛选重组子的方法。方法：不需抽提质粒,只要将菌落接入快检缓冲液后直接进行普通琼脂凝胶电泳分析,就可以快速筛选出重组子。结果：结果和提取质粒酶切鉴定结果一致。结论：经实验证明用快检缓冲液方法筛选重组子是一种简单易行,快速方便,节省时间,提高工作效率并且可靠的方法。     

一种简便的肌醇磷脂微量分析方法

分析磷脂酰肌醇循环(PI cycle)的磷脂组分常采用双向薄层层析法．建立了一个简单快速的单向薄层层析分离肌醇磷脂方法．首先采用不同的有机溶剂体系分别提取非多磷酸肌醇磷脂和多磷酸肌醇磷脂,然后用不同的层析展开体系,对两部分磷脂进行单向薄层层析分离．采用无载体 ³²P标记实验对该方法分离效果进行了观察．此法适用于同位素标记和非标记样品中肌醇磷脂组分的比较分析及多磷酸肌醇磷脂的提取、纯化和定量．     

一种简单、快速提取DNA的方法

随着分子生物学研究的迅速发展,提取ＤＮＡ已成为一项常规实验。用经典方法提取ＤＮＡ［１］,先用蛋白酶Ｋ、ＳＤＳ消化,然后用酚、氯仿抽提,乙醇沉淀,耗时较多,提取液需要多次转移,易引起交叉污染和ＤＮＡ丢失。本文利用硅藻（ｄｉａｔｏｍ）能够特异性吸附核酸的...     

简便快速分离天花粉毒蛋白的一种方法

 简便快速分离天花粉毒蛋白的一种方法孙建忠,季瑞华,王克夷（中国科学院上海生物化学研究所,上海２０００３１）天花粉是由多年生草质藤本植物栝楼（ＴｒｉｃｈｏｓａｎｔｈｅｓｋｉｒｉｌｏｗｉｉＭａｘｉｍ,Ｃｕｃｕｒｂｉｔａｃｅａｅ）的块茎制成,天花粉毒蛋白是...     

一种简单快速植物组织冰冻切片方法

比较不同冷冻方法对植物细胞超微结构的影响,结果表明:直接包埋法处理的植物细胞超微结构保存较好,而液氮冷冻处理的植物细胞内膜系统损伤严重.建立了一种直接包埋冷冻和适当回温相结合的方法,不仅可以制作出植物细胞基本结构保存完整的组织切片,而且避免了使用冰冻保护剂的弊端.其操作程序是:样品固定→冰冻与包埋→适当回温→快速切片→展片→染色.此法制作的切片可进行不同的染色和组织细胞化学测定,具有操作简便,易于推广的特点.     

A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation

An electroporation procedure for the plasmid-mediated genetic transformation of intact cells of Streptococcus cremoris and Streptococcus lactis was performed. Ten different strains were transformed. The method was simple and rapid and yielded transformant colonies in 14 to 24 h. The method was optimized for S. lactis LM0230, and transformation frequencies of between 1 × 10⁴ and 5 × 10⁵ transformants per μg of purified plasmid (pMU1328) were achieved routinely. The optimized procedure involved lysozyme treatment of cells. Transformation of LM0230 occurred at comparable frequencies with pLS1 (4.4 kilobase pair [kbp]), pMU1328 (7.4 kbp), and pAMβ1 (26.5 kbp). Plasmid DNA isolated from transformants had not undergone detectable deletions or rearrangements. Transformation was possible with plasmid DNA which was religated after restriction endonuclease digestion. Phage DNA-dependent transfection of S. lactis LM0230 and S. lactis C6 was also achieved.     

一种简便、快速的大肠杆菌质粒转化方法

将受体菌与质粒DNA混匀直接在Ca2+离子选择平板上进行转化和筛选,其转化过程仅需2 min左右,并能得到105以上的转化效率, 可满足一般克隆工作的需要。 Abstract：After mixing the recipient cells and plasmids DNA, directly spread the mixture on selective media containing Ca2+. The whole process of transformation just needs 2 min or so, and could acquire the transformation efficiency of more than 105, which is enough to common gene cloning.     

A Rapid and Simple Method for Assaying Interferon1

A new procedure of assaying interferon (IF) has been developed. Cell suspension was dispensed into liquid scintillation counting vials together with IF sample. During an overnight incubation, the cells adhered sufficiently to the bottom of the vials and all the subsequent procedures were carried out without transfer of the cells from the vials. Vesicular stomatitis virus was inoculated and virus-specific RNA was labeled by adding ³H-uridine and actinomycin D to the medium. Incubation was terminated prior to completion of a single-step growth of the virus and radioactivity of the labeled cells in each vial was determined. The reciprocal of the IF dilution which reduced the radioactivity in viral RNA by 50% was taken as the titer. The present procedure consists of simple manipulations and can be completed within 24 hr. Furthermore, it is quite reproducible and gives a titer almost identical to that obtained by the conventional plaque-reduction dose method. The procedure can be applied to mouse L cells, rabbit RK-13 cells and human FL cells, without modification.     

A Simple Method for Isolation and Photography of Clostridium Botulinum Colonies from Surface Cultures on Agar Plates

Reflected light gives a very good contrast using blood agar plates in an obliquous position under a stereoscopic microscope. Thus it is possible to isolate pure colonies of Cl. botulinum in the early stage of the growth on blood agar plates.     

一种简便、快速的大肠杆菌质粒转化方法

将受体菌与质粒DNA混匀直接在Ca2+离子选择平板上进行转化和筛选,其转化过程仅需2 min左右,并能得到105以上的转化效率, 可满足一般克隆工作的需要。 Abstract：After mixing the recipient cells and plasmids DNA, directly spread the mixture on selective media containing Ca2+. The whole process of transformation just needs 2 min or so, and could acquire the transformation efficiency of more than 105, which is enough to common gene cloning.     

血液基因组DNA的快速提取方法

目的:研究血液中基因组DNA的简便快速提取方法。方法:取新鲜抗凝血,以红细胞裂解液除去红细胞,再破碎白细胞,除去杂蛋白,获得基因组DNA。结果:所得基因组DNA完整、无断裂,含量和纯度均较高。以所提基因组DNA作为模板能很好的扩增出p21因子启动子序列,因此该法所提取的DNA是完整可靠的。结论:该法能简便、快速、安全、廉价的提取血液中的基因组DNA,并适用于临床检测和分子生物学研究。     

一种简便快速的聚合酶活性实时检测新方法

基于双链DNA结合染料能特异嵌入双链DNA发出荧光的原理,发展了一种实时检测DNA聚合酶活性的简便方法.在检测过程中,聚合酶的聚合反应进程被实时转换为荧光信号,通过监测荧光强度的变化实时检测聚合酶的活性及药物对聚合酶活性的影响.该方法不需要对DNA进行放射性同位素标记和荧光标记,也不需要聚丙烯酰胺凝胶电泳和聚合酶链式反应,是一种简便、快速的聚合酶活性实时检测新方法,为研究抗肿瘤药物对聚合酶活性的影响提供了一种简捷方法,也将为相关疾病诊治和药物筛选提供一种新的思路.