Lifelong dynamics of human CD4CD25 regulatory T cells: Insights from in vivo data and mathematical modeling

Despite their limited proliferation capacity, regulatory T cells (T_regs) constitute a population maintained over the entire lifetime of a human organism. The means by which T_regs sustain a stable pool in vivo are controversial. Using a mathematical model, we address this issue by evaluating several biological scenarios of the origins and the proliferation capacity of two subsets of T_regs: precursor CD4⁺CD25⁺CD45RO⁻ and mature CD4⁺CD25⁺CD45RO⁺ cells. The lifelong dynamics of T_regs are described by a set of ordinary differential equations, driven by a stochastic process representing the major immune reactions involving these cells. The model dynamics are validated using data from human donors of different ages. Analysis of the data led to the identification of two properties of the dynamics: (1) the equilibrium in the CD4⁺CD25⁺FoxP3⁺T_regs population is maintained over both precursor and mature T_regs pools together, and (2) the ratio between precursor and mature T_regs is inverted in the early years of adulthood. Then, using the model, we identified three biologically relevant scenarios that have the above properties: (1) the unique source of mature T_regs is the antigen-driven differentiation of precursors that acquire the mature profile in the periphery and the proliferation of T_regs is essential for the development and the maintenance of the pool; there exist other sources of mature T_regs, such as (2) a homeostatic density-dependent regulation or (3) thymus- or effector-derived T_regs, and in both cases, antigen-induced proliferation is not necessary for the development of a stable pool of T_regs. This is the first time that a mathematical model built to describe the in vivo dynamics of regulatory T cells is validated using human data. The application of this model provides an invaluable tool in estimating the amount of regulatory T cells as a function of time in the blood of patients that received a solid organ transplant or are suffering from an autoimmune disease.     

Babesia bigemina: Advances in continuous in vitro culture using serum-free medium supplemented with insulin,transferrin, selenite,and putrescine

This study reported that Babesia bigemina (Bbig-SF) was continuously cultured in vitro in a serum-free medium supplemented with a mixture of insulin-transferrin-selenite (M-ITS) and putrescine (Pu). Firstly, the effect of five different types of basal culture media supplemented with 40% bovine serum was evaluated regarding the proliferation of the protozoan parasite. Cultures with the advanced DMEM/F12 medium (A-DMEM/F12) showed the highest percentage of parasitized erythrocytes (PPE) at 8.37%. Using A-DMEM/F12, a strain of B. bigemina (Bbig-SF) was adapted for growth in bovine serum-free medium by a sequential reduction of serum and demonstrated a maximum PPE of 7.18% in the absence of serum. The next study was the evaluation of the effect of adding four different concentrations of M-ITS to the serum-free A-DMEM/F12 medium on Bbig-SF; the optimal concentrations of M-ITS were 2000, 1100, and 1.34 mg/L, which yielded a PPE of 7.23%. Next, eight levels of Pu were evaluated on Bbig-SF cultured in serum-free A-DMEM/F12. After the addition of 0.1012 mg/L of Pu, the maximum PPE was 7.61%. When the combination of serum-free A-DMEM/F12 + M-ITS (2000, 1100, and 1.34 mg/L) + Pu (0.1012 mg/L) was evaluated, it yielded a maximum PPE of 14.80%. Finally, the combination of M-ITS + Pu in A-DMEM/F12 without serum and incorporation of a perfusion bioreactor yielded a maximum PPE of 33.45%. We concluded these culturing innovations for B. bigemina in vitro allow the optimization of small- and large-scale proliferation as a source of this protozoan parasite for future studies.