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1.
M. Tth C. Lfstedt B.S. Hansson G. Szcs A.I. Farag 《Entomologia Experimentalis et Applicata》1989,51(2):107-112
Four compounds, tetradecyl acetate, (Z)-9-tetradecenyl acetate, (E)-11-tetradecenyl acetate and (Z)-11-tetradecenyl acetate were identified from female sex pheromone extracts of Hungarian and Egyptian lima-bean pod borers (Etiella zinckenella Tr., Lepidoptera: Phycitidae) by gas chromatography with flame ionization (FID) and electroantennographic (EAD) detection. In EAG studies these monoun-saturated acetates gave the best responses in a series of other tetradecenyl acetates and tetradecenols. The four component blend of the identified components in similar ratios as in the pheromone extract attracted significant numbers of male lima-bean pod borers in both Hungary and Egypt. In a preliminary subtraction test best capture was achieved by the ternary mixture of the monounsaturated acetates.
Résumé A partir de femelles d'E. zinckenella d'origines hongroise et égyptienne, nous avons isolé quatre composés par chromatographie en phase gazeuse avec ionisation de flamme et électroantennographie (EAD): l'acétate de tétradécanyl, l'acétate (Z)-11-tétradécényl, l'acétate (E)-11-tétradécényl et l'acétate (Z)-11-tétradécényl. Les acétates monoinsaturés donnent les meilleures réponses en EAG parmi une série d'acétates tétradécényls et de tétradécénols. Les quatre composés mélangés dans les mêmes proportions que dans l'extrait de la phéromone ont attiré un nombre significatif de mâles tant en Egypte qu'en Hongrie. Dans un test préliminaire de soustraction, la meilleure capture a été réalisée par le mélange ternaire d'acétates monoinsaturés.相似文献
2.
P. Witzgall W. Sauter H.-R. Buser S. Rauscher H. Arn P. J. Charmillot Th. Wildbolz 《Entomologia Experimentalis et Applicata》1989,53(2):133-136
As shown by chemical analysis and field trapping, the sex pheromone of Grapholita lobarzewskii consists of a blend of (E)-8-dodecenyl acetate and (Z)-8-dodecenyl acetate. Maximum attractiveness was found at 80–95%. Dodecyl acetate and tetradecyl acetate, both present in the female gland, did not affect trap catch. G. lobarzewskii has recently gained importance as a pest of apple and plum, but has so far been referred to as Grapholita janthinana. The latter is attracted to the same two compounds, but in reversed portions (20% E).
Zusammenfassung Chemische Analysen und Feldversuche zeigen, dass das Sexualpheromon des Kleinen Fruchtwicklers, G. lobarzewskii aus (E)-8-Dodecenylacetat (Z8-12:Ac) und (Z)-8-Dodecenylacetat (Z8-12:Ac) besteht. Die grösste Lockwirkung wurde mit einem Gemisch der beiden Substanzen bei einem E-Anteil von 80–95% erzielt. Dodecylacetat (12:Ac) und Tetradecylacetat (14:Ac) wurden ebenfalls in den weiblichen Pheromondrüsen nachgewiesen, hatten aber im Freiland keinen Einfluss auf die Lockwirkung. G. lobarzewskii tritt seit einiger Zeit in der Schweiz als Schädling von Apfel und Zwetschge auf und ist an ihrem charakteristischem Frassgang leicht erkennbar. Die Art wurde bisher irrtümlich als G. janthinana bezeichnet. G. janthinana lebt auf Rosaceen, und wird vom gleichen Substanzpaar angelockt, allerdings bei umgekehrtem Mischungsverhältnis (20% E).相似文献
3.
The sex pheromones produced by virgin females of three species of small ermine moths occurring sympatrically on the European spindle tree were analysed by gas chromatography and the synthetic compounds tested against male moths in the laboratory and field. Pheromone gland extracts of Yponomeuta cagnagellus were shown to contain tetradecyl acetate, (E)-11- and (Z)-11-tetradecenyl acetate, tetradecanol, (E)-11- and (Z-11-tetradecenol and hexadecyl acetate in 30/3/100/6/0/7/42) ratio. Wind tunnel bioassays and field tests showed that (E)-11-tetradecenyl acetate primary pheromone components, and that tetradecyl acetate synergised their attractiveness. The alcohols were unimportant in these tests. Analysis of the pheromone gland extracts from Y. irrorellus showed the above components in 68/56/100/9/6/8/17 ratio, and a mixture of these attracted male moths in laboratory and field. Omission of either unsaturated acetate gave unattractive mixtures, and the alcohols were also found to be important for attraction. Pheromone gland extracts from Y. plumbellus were shown to contain the seven components in 46/148/100/20/20/13/25 ratio. Field tests showed that the two monounsaturated acetates are primary pheromone components, and removal of the alcohols had no significant effect. The titre of (Z)-11-tetradecenyl acetate in Y. plumbellus pheromone gland extracts was approximately 0.5 ng per female, one tenth the titre in the other two species. It is concluded that mixtures of three or more pheromone components with specific E/Z ratio are essential for full attraction and contribute towards premating reproductive isolation of the three species. Other factors such as rate of pheromone emission, time of flight and height of flight may also contribute to reproductive isolation.
Résumé L'analyse a porté sur les phéromones sexuelles de 3 Yponomeutidae, trouvés en sympatrie sur le fusain. Des expériences sur le comportement de Y. cagnagellus ont montré que la fraction acétate de tétradécyl de la phéromone synergise les fractions connues antérieurement: (E)-11- et (Z)-11-acétates de tétradécényl, lorsque les proportions sont 37/2/100. L'analyse en chromatographie gazeuse d'extraits de glandes à phéromone de Y. irrorellus a mis en évidence, dans les proportions approximatives suivantes 68/56/100/9/6/8/7, de l'acétate de tétradécyl, des (E)-11 et (Z)-11 acétates de tétradécényl, du tétradécanol, des (E)-11 et (Z)-11-tétradécénols, et de l'acétate d'hexadécyl. Un mélange de ces composés a provoqué un vol intégral contre le vent des mâles dans un tunnel à vent et a attiré les mâles dans la nature.L'analyse des extrait de glandes à phéromone de Y. plumbellus a donné de l'acétate de tétradécyl, du (E)-11-acétate de tétradécényl et du (Z)-11-acétate de tétradécényl, dans les proportions: 50/150/100. Une étude par élimination dans la nature a montré que les acétates non saturés étaient les composés fondamentaux de la phéromone. La teneur en (Z)-11-acétate de tétradécényl de la glande à phéromone de Y. plumbellus était d'environ 0.5 ng par femelle, soit approximativement le dixième de celle observée chez les deux autres espèces.Une très faible attraction croisée a été trouvée avec des phéromones totalement synthétisées. Lorsque l'acétate de (E)-11-tétradécényl a été enlevé des phéromones de Y. irrorellus et Y. plumbellus, le reliquat a attiré des nombres significatifs de Y. cagnagellus. Nous en concluons que des mélanges de trois composés ou plus, avec des proportions spécifiques des isomères (E) et (Z), sont indispensables pour une activité complète et pour maintenir un isolement reproductif précopulatoire entre les espèces.相似文献
4.
Gabor Szöcs Meyer Schwarz György Sziraki Miklos Toth Jerome A. Klun Barbara A. Leonhardt 《Entomologia Experimentalis et Applicata》1985,39(2):131-133
(E,Z)-2,13-octadecadien-1-ol acetate and (Z)-13-octadecen-1-ol acetate have been identified from the ovipositor extracts of the currant borer, Synanthedon tipuliformis (Clerck), by capillary GC, and the structures were verified by GC-MS. The diene/monoene ratio in the extract was found to be 93:7. Mixtures of these two compounds as well as the diene alone attracted conspecific males in the field.
Zusammenfassung (E,Z)-2,13-Octadecadienylacetat und (Z)-13-Octadecenylacetat wurden in Ovipositor-Extrakten des Johannisbeerglasflüglers Synanthedon tipuliformis kapillar-gaschromatographisch nachgewiesen. Die Strukturen wurden mittels Gaschromatographisch-Massenspektrometrie bestätigt. Das Verhältnis Dien/Monoen im Extrakt betrug 93:7. Mischungen der beiden Komponenten wie auch (E,Z)-2,13-Octadecadienylacetat allein lockten im Freiland Männchen dieser Glasflüglerart an.相似文献
5.
Alan Cork Peter S. Beevor David R. Hall Brenda F. Nesbitt Gertrudo S. Arida Osamu Mochida 《Entomologia Experimentalis et Applicata》1985,37(2):149-153
Ovipositor washings and volatiles from virgin female Scirpophaga incertulas (Walker) (Lepidoptera: Pyralidae) were analysed by gas chromatography (GC) linked to electroantennography (EAG). Two EAG responses were observed at retention times consistent with those of monounsaturated 16-carbon aldehydes and monounsaturated 16-carbon alcohols. Analysis of ovipositor washings on high-resolution, capillary GC columns showed peaks which cochromatographed with (Z)-11-hexadecenal, (Z)-9-hexadecenal, (Z)-11-hexadecen-1-ol and hexadecanal. EAG responses of male S. incertulas to synthetic hexadecenal isomers were greatest for the (Z)-11 and (Z)-11 isomers. Field tests in the Philippines showed that traps baited with a mixture of (Z)-11-hexadecenal and (Z)-9-hexadecenal in the naturally occurring 3:1 ratio caught significantly more male S. incertulas moths than those baited with a virgin female moth. Addition of (Z)-11-hexadecen-1-ol to this mixture reduced trap catches.
Résumé Des produits provenant du lavage d l'ovipositeur et des substances volatiles de femelles vierges de S. incertulas Walker (Lep.: Pyralidae) ont été examinées en chromatographie en phase gazeuse (GC) avecélectroantennogramme simultané(EAG). Deux réponses EAG ont été enregistrées à des temps de rétention correspondant à ceux des aldéhydes monoinsaturés 16 carbones et des alcools monoinsaturés 16 carbones. L'analyse des produits provenant du lavage de l'ovipositeur en colonnes GC capillaires à haute résolution a donné des pics correspondant aux (Z)-11-hexadécenal, (Z)-9-hexadécenal, (Z)-11-hexadecen-1-ol et à l'hexadecanal. Les réponses EAG de mâles de S. incertulas aux isomères synthétiques d'hexadécenal étaient plus intenses pour les isomères (Z)-11 et (E)-11. Des essais en champ aux Philippines ont montré que les pièges contenant un mélange de (Z)-11-hexadécenal et de (Z)-9-hexadécenal dans le rapport natural 3/ 1 capturaient plus de mâles de S. incertulas que ceux contenant une femelle vierge. L'addition au mélange de (Z)-11-hexadecen-1-ol réduit les captures.相似文献
6.
Miklós Tóth Gábor Szócs Christer Löfstedt Bill S. Hansson Mitko Subchev 《Entomologia Experimentalis et Applicata》1986,42(3):291-299
(Z)-11-hexadecenyl acetate (Z-11-16:Ac), (Z)-11-hexadecenal (Z-11-16:Ald), (Z)-11-hexadecenol (Z-11-16:OH) and hexadecanyl acetate (16:Ac) were found in pheromone gland extracts of femaleMamestra suasa (Den. et Schiff.) in the relative amounts 100/2/10/5. All four compounds were also present in collections of aiiborne volatiles from calling females in a 100/7/5/5 ratio. No traces of 14 carbon aldehydes or acetates were detected. In gland extracts the presence of methyl hexadecanoate methyl (Z)-9-hexadecenoate and methyl (Z)-11-hexadecenoate was demonstrated by base methanolysis. No methyl tetradecenoates were detected. In EAG tests Z-11-16:Ac gave the best responses, followed by (Z)-9-tetradecenyl acetate (Z-9-14:Ac) Z-11-16:Ald and Z-11-16:OH. In single sensillum recordings large spike amplitude cells in sensilla responded to Z-11-16:Ac, while small spike amplitude cells to both Z-11-16:OH and Z-9-14:Ac. Cells responding to Z-11-16:Ald were found in one out of 60 sensilla tested. In wind tunnel tests 0.1 g of a 10:1 blend of Z-11-16:Ac/Z-11-16:Ald evoked the same responses and at a similar intensity as 3 isolated female pheromone glands did. In field tests a 10:1 blend of Z-11-16:Ac/Z-11-16:Ald caught significant numbers of males in both Bulgaria and Hungary. The addition of 16:Ac to the binary blend did not have any effect, while more than 1% of Z-11-16:OH or 0.1% of Z-9-14:Ac dramatically decreased captures. In comparing different ratios of the, acetate/aldehyde blend at different dose levels, best catches were recorded at the 10:1 ratio and at the highest (1000 g) dose level.
La composition de la phéromone sexuelle deMamestra suasa: analyse chimique, étude de l'effet par éléctrophysiologie et à la chambre de vol, et piégeages dans deux pays de l'Europe
Résumé On a trouvé l'acetoxy-1 hexadécene-11 Z (Z-11-16:Ac), le hexadécene-11 Z al-1 (Z-11-16:Ald), le hexadécene-11 Z ol-1 (Z-11-16:OH) et l'acetoxy-1 hexadécene (16:Ac) dans des extraits de glandes phéromona les des femelles deMamestra suasa. La proportion relative des composés était 100/2/10/5. Tous les quatre composés ont été présents aussi dans les collections d'émanations des femelles en stade d'appel, dans la proportion un peu différente de 100/7/5/5. On n'a détecté aucune trace des tétradécenes al-1 ou d'acetoxy-1 tétradécenes. On a démontré la présence de hexadécenoate-1 methyl, hexadécene-9 Z oate-1 methyl et héxadécene-11 Z oate-1 methyl dans des extraits des glandes, par la méthode de base methanolysis. On n'a trouvé pas des tétradéceneoates methyl.En éléctroantennographie, Z-11-16:Ac a donné les meilleurs réponses, suivis par l'acetoxy-1 tétradécene-9 Z (Z-9-14:Ac), Z-11-16:Ald et Z-11-16:OH. Dans des études de single sensillum les cellules à amplitude grande ont répondu à la stimulation avec de Z-11-16:Ac, cependant les cellules à amplitude petite ont répondu à la stimulation avec des deux composés Z-9-14:Ac et Z-11-16:OH. On a trouvé des cellules sensitives à Z-11-16:Ald dans 1 entre 60 sensilla étudiés.À la chambre de vol, le dose de 0.1 g d'un mélange de 10:1 de Z-11-16:Ac/Z-11-16:Ald a provoqué les mêmes réponses et à l'intensité pareille comme 3 glandes phéromonales isolées des femelles.En piégeages sur le champs des males en quantité importante ont été capturé par un mélange de 10:1 de Z-11-16:Ac/Z-11-15:Ald en Bulgarie et Hongrie. L'addition de 16:Ac au mélange binaire n'avait aucun effet, cependant l'addition de plus de 1% de Z-11-16:OH ou 0.1% de Z-9-14:Ac a sérieusement diminué les captûres. En comparant des proportions différentes du mélange de l'acetoxy/aldéhyde dans des doses différentes, on a observé les meilleurs captûres avec de la proportion 10:1 et à la dose la plus haute (1 000 g).相似文献
7.
Brigitte Frérot Philippe Lucas Didier Rochat 《Entomologia Experimentalis et Applicata》1989,53(1):81-87
The upwind flight and landing responses of male Mamestra suasa (Den. & Schiff.) (Lepidoptera; Noctuidae) to various synthetic chemicals were studied in a wind tunnel. A mixture of (Z)-11-hexadecenyl acetate (Z11-16: Ac) and (Z)-11-hexadecenal (Z11-16: Ald) elicited upwind flight responses past the midpoint of the wind tunnel, and both components were necessary for landing of the males to occur at the pheromone source point. The best response to the mixtures tested was to a ratio 100:1 (ng) of Z11-16: Ac and Z11-16: Ald (blend B).The addition of (Z)-9-tetradecenyl acetate (Z9-14: Ac) (10 ng) to blend B resulted in a significant decrease of upwind flight and landing responses. The inhibitory effect of this chemical when it is mixed with blend B, and the presence of a specific receptor for this compound in sensilla trichodea of male M. suasa, suggest that Z9-14: Ac is involved in the chemical communication between M. suasa and other sympatric species. The addition of (Z)-11-hexadecenol (Z11-16: OH) (1 or 10 ng) to blend B had no effect on male upwind flight and landing responses. This compound does not seem to be involved in the chemical communication of this species.
Résumé L'observation et la comptabilisation des vols orientés et des atterrissages des mâles de Mamestra suasa (Den. & Schiff.) (Lepidoptera; Noctuidae) sur une source de phéromone de synthèse ont mis en évidence un mélange attractif appelé mélange B et constitué par 100 ng de Z11-16: Ac et 1 ng de Z11-16: Ald. L'attraction et l'atterrissage sont sous la dépendance du mélange de ces 2 composés. Les variations de leurs proportions relatives affectent les différentes phases du comportement de vol des mâles.L'addition de 10 ng d'acétoxy-1 tetradécène-9 Z (Z9-14: Ac) diminue significativement les proportions de vols orientés et d'atterrissages par rapport aux réponses obtenues avec le mélange B seul. L'effet inhibiteur de cette molécule sur l'attractivité du mélange B et la présence chez M. suasa d'un récepteur spécialisé dans la perception de ce composé suggèrent que le Z9-14: Ac est impliqué dans la communication chimique entre M. suasa et d'autres espèces sympatriques. L'addition d'hexadécènol-11 Z (Z11-16: OH) au mélange B à la dose de 1 ou 10 ng ne modifie pas les réponses des mâles. Ce composé ne semble pas impliqué dans la communication chimique chez M. suasa.相似文献
8.
M. T. Smith L. M. McDonough S. Voerman H. G. Davis R. D. Eikenbary C. L. Smithhisler 《Entomologia Experimentalis et Applicata》1987,44(1):23-30
Electroantennogram (EAG) measurement of male Cydia caryana moth antennal olfactory response to monounsaturated 12 and 14 carbon alcohols and acetates indicated that the (E)-8-, (E)-10- conjugated double bond system of a dodecadien-1-ol acetate is a critical chemical structural component of the C. caryana sex pheromone. Additionally, EAG measurements implicated (E)-8-dodecen-1-ol acetate, (Z)-8-dodecen-1-ol acetate, (Z)-9-dodecen-1-ol acetate and (Z)-12-tetradecen-1-ol as potential minor pheromonal components. An EAG dosage-response study suggested that there were at least two heterologous populations of pheromone acceptors. Behavioral analysis of male moth response in a flight tunnel to compounds which evoked the stronger EAG responses suggested that (E,E)-8,10-dodecadien-1-ol acetate and (Z)-9-dodecen-1-ol acetate resemble or are C. caryana sex pheromonal components, while (Z)-8-dodecen-1-ol acetate and (E)-10-dodecen-1-ol acetate are either parapheromones or are minor pheromone components. Behavioral significance of (Z)-12-tetradecen-1-ol was difficult to interpret in the flight tunnel.
Résumé Les réponses olfactives antennaires de Cydia caryana, mesurées par électroantennogrammes (EAG), aux alcools et acétates à carbones monounsaturés en positions 12 et 14, ont montré que le système conjugué de double liaison, (E)-8-, (E)-10- du dodecadien-1-ol acétate constitue un composé chimique strutural critique de la phéromone sexuelle de C. caryana.De plus, les acétates: (E)-8-dodecen-1-ol,(Z)-8-dodecen-1-ol,(Z)-9-dodecen-1-ol, et le (Z)-12-tetradecen-1-ol, se sont révélés en AEG comme des composés secondaires de la phéromone. L'étude par AEG de la relation dose-réponse a conduit à l'hypothèse de deux catégories de populations de récepteurs de phéromones. L'analyse comportementale des résponses des papillons mâles dans le tunnel de vol aux composés qui ont provoqués les plus forts AEG, on fait estimer que les acétates (E,E)-8,10-dodécadien-1-ol et (Z)-9-dodecen-1-ol ressemblent (ou sont) les constituants de la phéromone sexuelle de C. caryana; tandis que les (Z)-8-dodecen-1-ol et (E)-10-dodecen-1-ol sont, soit des paraphéromones, soit des constituants mineurs de la phéromone.La signification biologique du (Z)-12-tétradécen-1-ol a été difficile à interprêter avec les expériences en tunnel de vol.相似文献
9.
Thomas C. Baker Wendy Meyer Wendell L. Roelofs 《Entomologia Experimentalis et Applicata》1981,30(3):269-279
The sex pheromone-mediated responses of male oriental fruit moths, Grapholitha molesta (Busck), to an array of blends and dosages of Z8-12Ac and E8-12:Ac, were analyzed. Males exhibited sustained upwind flight resulting in source location only to intermediate blends and dosages. This optimal range of treatments appeared to be bounded by dosages too low or too high to result in significant attraction (net within-plume displacement toward the source), the higher concentrations causing arrestment (no net within-plume displacement) at some distance from the source. Similar results were obtained in field-trapping studies with the same treatments, except that the optimal dosages were moved up about 10-fold. Increased turning and decreased linear velocity could account for arrestment with increasing amounts of the (E) isomer in high dosages and high (E)/(Z) ratio blends.
Zusammenfassung Das Sexualverhalten männlicher Falter des Pfirsichtriebwicklers, Grapholitha molesta (Busck), gegenüber verschiedenen Dosierungen und Mischungen von (Z)-8- und (E)-8-Dodecenylacetat wurde untersucht. Ein stetiger Aufwindflug, der zum Auffinden der Duftquelle führte, konnte nur im mittleren Dosierungs- und Mischungsbereich beobachtet werden. Ausserhalb dieses Optimums war die Dosierung offenbar entweder zu hoch oder zu niedrig, um eine deutliche Anlockung, d.h. einen gerichteten Flug innerhalb der Duftfahne zu bewirken; bei hoher Konzentration näherten sich die Tiere der Quelle nur auf bestimmte Distanz (arrestment). Freilandversuche mit Fallen führten zu ähnlichen Ergebnissen, nur dass dort das Dosierungsoptimum bei ca. zehnmal höheren Werten lag als in den Versuchen im Windkanal. Der bei hohen Mengen an E-Isomer (hohe Dosis oder hohes E/Z-verhältnis) beobachtete Arrestment-Effekt könnte auf Erhöhung der Drehbewegungen oder Verringerung der Fluggeschwindigkeit zurückzuführen sein.相似文献
10.
Sex pheromone of Etiella behrii, a pod borer of soybean in Indonesia: identification and field attraction 总被引:1,自引:0,他引:1
Sadao Wakamura Makoto Hattori Kazunori Igita Koji Yasuda Tridjaka 《Entomologia Experimentalis et Applicata》1999,91(3):413-420
Four EAG-active components were detected in GC-EAG analyses of hexane extracts from virgin Etiella behrii (Zeller) (Lepidoptera: Pyralidae) females. These components were identified as dodecyl acetate (12:Ac), (E)-9-dodecenyl acetate (E9-12:Ac), either (Z)-9- or (E)-11-tetradecenyl acetate (Z9- or E11-14:Ac), and (Z)-11-tetradecenyl acetate (Z11-14:Ac) by comparison of retention indices on both nonpolar and polar GC columns. The available extract was insufficient for further GC-MS or other chemical analyses (<0.2 ng/female). In field tests carried out in East Java, a 10:90 mixture of E9-12:Ac and Z11-14:Ac showed attractiveness to male moths and addition of 12:Ac and/or E11-14:Ac significantly increased the trap catches while addition of Z9-14:Ac showed no significant effect. Maximum attraction was obtained with 5.35 or 10.7 g/rubber septum of a mixture of E9-12:Ac, Z11-14:Ac, 12:Ac and E11-14:Ac at the ratio of 10:90:0.7:6.3, respectively. The role of pheromone blends in species discrimination between E. behrii and the related E. zinckenella (Treitschke) is discussed. 相似文献
11.
Isabelle Sreng Thomas Glover Wendell Roelofs 《Archives of insect biochemistry and physiology》1989,10(1):73-82
Previous attempts to shift the (Z)-11-/(E)-11-tetradecenyl acetate ratio of pheromone components in the redbanded leafroller moth (RBLR), Argyrotaenia velutinana, by several selection protocols showed that this ratio is strongly canalized. Analysis of the complete seven-component blend, however, showed that a Geneva laboratory stock of RBLR had a lower percent (20%) of the E9–12:OAc minor component compared to the E11–14:OAc component than a population of RBLR from North Carolina (31%). Hybrid populations from these two cultures were used in a two-way family truncation selection scheme in which families were selected for either the lowest (low line) or the highest (high line) ratio of E9–12:OAc/E11–14:OAc. After three generations of selection, the low line had 14% E9–12:OAc relative to E11–14:OAc and the high line had 42%. The selection pressure was removed in generations 4–9, and the low line remained unchanged at 14% E9–12:OAc; but in the high line, it drifted to 53%. Studies were conducted to estimate heritability and realized heritability. The realized heritability calculated for each generation of selection averaged 1.14 for the low line and 1.50 for the high line. These calculations, along with estimated heritability values of 0.416 and 0.644 for reciprocal crosses, indicate some plasticity in the E9–12:OAc/E11–14:OAc ratio. This ratio was positively correlated to the total amount of 12-carbon components to 14-carbon components, but was negatively correlated to the Z/E ratio of Δ11-tetradecenylacetates. The results of two studies on the canalization of various components of the RBLR sex pheromone blend indicate that there is limited potential in this insect for manipulation of the blend ratios in the laboratory. 相似文献
12.
David R. Hall Peter S. Beevor Alan Cork Brenda F. Nesbitt Eric A. S. La Croix 《Entomologia Experimentalis et Applicata》1984,35(1):33-36
Ovipositor washings from virgin female Cryptophlebia batrachopa (Meyrick) (Lepidoptera: Tortricidae) were analysed by gas chromatography (GC) linked to electroantennography (EAG) and by high-resolution GC. The major EAG-active component in ovipositor washings was shown to be (Z)-8-dodecenyl acetate with less than 0.5% of the E isomer. GC analysis of ovipositor washings from C. leucotreta confirmed the presence of approximately equal amounts of (Z)- and (E)-8-dodecenyl acetates (Persoons et al., 1977). When pure (Z)-8-dodecenyl acetate and mixtures containing up to 50% of the E isomer were tested in the field in Malawi where both Cryptophlebia species occurred, C. batrachopa male moths were caught only in traps baited with 10% or less of the E isomer and highest catches were recorded for the pure Z isomer. By contrast C. leucotretra males were caught predominantly by traps baited with more than 10% E isomer and no catches were recorded for the pure Z isomer. (Z)-8-dodecenyl acetate containing 1% of the E isomer was found to attract male moths of Opogona spp.
Résumé Les produits recueillis par lavages de l'ovipositeur de Cryptophlebia batrachopa Meyr. (Lep. Tortricidae) ont été analysés par chromatographie gazeuse (GC) associée à l'électroantennographie (EAG) et la GC à haute résolution. Le principal constituant actif en EAG provenant des lavages s'est révélé être le (Z)-8-dodécényl acétate avec moins de 0,5% de l'isomère E. L'analyse GC de ces produits de lavage de C. leucotreta ont confirmé la présence d'à peu près les mêmes quantités de (Z) et (E)-8-dodécényl acétates (Persoons et al., 1977). Quand des essais sur le terrain ont été effectués au Malawi, où existent les deux espèces de Cryptophlebia, avec du (Z)-8-dodécényl acétate et des mélanges comprenant jusqu'à 50% de l'isomère E, des mâles de C. batrachopa ont seuls été capturés dans des pièges contenant 10% ou moins de l'isomère E; les captures les plus élevées ont été obtenues avec l'isomère Z pur. A l'opposé, surtout des mâles de C. leucotreta ont été capturés dans des pièges garnis avec plus de 10% d'isomère E, et aucune capture n'a été obtenue avec l'isomère Z pur. Des mâles d'Opogona spp. ont été attirés par du (Z)-8-dodécényl acétate contenant 1% de l'isomère E.相似文献
13.
Yongping Huang Hiroshi Honda Yutaka Yoshiyasu Sugihiko Hoshizaki Sadahiro Tatsuki Yukio Ishikawa 《Entomologia Experimentalis et Applicata》1998,89(3):281-287
The sex pheromone blend of the butterbur borer, Ostrinia zaguliaevi (Lepidoptera: Pyralidae) was analyzed by means of gas chromatography-electroantennographic detection (GC-EAD), GC-mass spectrometry and a series of wind-tunnel bioassays. Four EAD-active compounds were detected in the female sex pheromone gland extract, and these were identified as tetradecyl acetate (14:OAc), (Z)-9-tetradecenyl acetate (Z9-14:OAc), (E)-11-tetradecenyl acetate (E11-14:OAc) and (Z)-11-tetradecenyl acetate (Z11-14:OAc). The average amounts ± s.d. of the four compounds in a single sex pheromone gland were 7.9±3.7 ng, 10.1±3.2 ng, 1.1±0.5 ng and 11.6±5.1 ng, respectively. In a wind-tunnel bioassay, the ternary blend of Z9-, E11- and Z11-14:OAc at a ratio found in the sex pheromone gland (45:5:50) elicited the same behavioral responses from the males as did virgin females and pheromone gland extract. Removal of any single compound from the ternary blend significantly diminished the pheromonal activity, whereas addition of 14:OAc to the ternary blend had no effect on the males' behavioral responses. Therefore, it was concluded that the sex pheromone blend of O. zaguliaevi is composed of Z9-14:OAc, E11-14:OAc and Z11-14:OAc at a ratio of 45:5:50. 相似文献
14.
C. Tonini G. Cassani P. Piccardi S. Maini P.L. Castellari E. Pasqualini 《Journal of insect physiology》1982,28(5):443-446
Female-tip washings of the leafroller moth, Pandemis cerasana, were found to contain (E)-11-tetradecenyl acetate, (Z)-11-tetradecenyl acetate, (E)-11-tetradecenol, (Z)-11-tetradecenol and tetradecyl acetate, based on chemical analysis and electroantennogram tests. The relative amounts of these compounds in the gland were ca. 64:21:10:3:2 in the order named. Only (E)-11 and (Z)-11-tetradecenyl acetate were required for attraction of males to trap dispensed in the ratio 3:1, respectively. 相似文献
15.
Pexicopia malvella (Hbn.) males were attracted to mixtures containing (Z,E)-7,11-hexadecadienyl acetate and (Z,Z)-7,11-hexadecadienyl acetate, in screening tests, in Hungary. An approximate dose of 40 g 5:1 ZE:ZZ mixture is recommended for practical use as a selective and potent sex attractant.
Platyedra subcinerea (Hw.), another gelechiid pest, was attracted best to a 300 g dose of (Z,Z)-7,11-hexadecadienyl acetate.
Zusammenfassung Die binäre Mischung von (Z,Z)-7,11-hexadekadienylacetat und (Z,E)-7,11-hexadekadienylacetat erwies sich im Freiland als Sexuallockstoff für Männchen von P. malvella (Gelechiidae, Lepidoptera). Die Verhältnisse 1:1, 1:1.4, 1:2.5, 1:5 und 1:10 lockten bedeutende Anzahlen von Männchen, die Komponenten allein waren unwirksam. Die Mischung in Verhältnis 1:5 und bei der Köderbeladung von ungefähr 40 g kann zur praktischen Anwendung als ein spezifisches und effektives Lockstoff empfehlt werden.Männchen von P. subcinerea (= vilella Gelechiidae, Lepidoptera) wurden zu Fallen mit dem Isomer (Z,Z) angelockt. Beste Fangergebnisse wurden mit der Köderbeladung 300 g (Z,Z)-7,11-hexadekadienylacetat erreicht. P. malvella wird in Ungarn als bedeutungsvoller Schädling von Zierpflanzen der Familie Malvaceae gemeldet, und alle beide Arten sind als Baumwolleschädlinge in zentralasiatischen Sovietrepubliken und anderen Ländern in Asien bekannt.相似文献
16.
A. Ganeswara Rao D. D. R. Reddy K. Krishnaiah P. S. Beevor A. Cork D. R. Hall 《Entomologia Experimentalis et Applicata》1995,74(3):195-200
Analysis of ovipositor washings from virgin femaleCnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae) of Indian origin by linked gas chromatography and electroantennography indicated the presence
of three electrophysiologically-active compounds. These were identified on the basis of their gas chromatographic retention
times and mass spectra as (Z)-11-hexadecenyl acetate, (Z)-13-octadecenyl acetate and (Z)-13-octadecen-1-ol with (Z)-13-octadecenyl acetate present in amounts of between 0.25 and 1.5 ng per ovipositor and the other two components at less
than 10% of this. Trace quantities of octadecyl acetate were identified by mass spectrometry but no electroantennographic
responses were observed to this compound. Field trials conducted with a range of blends of the three electrophysiologically-active
compounds showed that blends containing between 5% and 30% (Z)-11-hexadecenyl acetate in (Z)-13-octadecenyl acetate dispensed from either white rubber septa or polythene vials were more attractive to male moths than
a virgin female moth. Addition of (Z)-13-octadecen-1-ol reduced attractiveness to male moths in the blends and concentrations tested. 相似文献
17.
Brenda F. Nesbitt Peter S. Beevor Alan Cork David R. Hall Ruth M. Murillo Helmuth R. Leal 《Entomologia Experimentalis et Applicata》1985,38(1):81-85
The sex pheromone produced by virgin female S. solanivora moths has been shown to contain (E)-3-dodecenyl acetate with approximately 2% of the Z isomer by electroantennography and gas chromatography. In the field, traps baited with (E)-3-dodecenyl acetate alone or in combination with 1% or 2% of the Z isomer caught at least as many male S. solanivora moths as those baited with virgin female moths, and there was some evidence that addition of 5% of the Z isomer reduced catches.
Résumé S. solanivora est l'un des plus importants lépidoptères détruisant les pommes de terre en Amérique Centrale. L'analyse par chromatographie en phase gazeuse (GC) des produits obtenus par lavage de l'ovipositeur de femelles vierges, associée à des électro-anténnogrammes (EAG) a révélé un composé actif unique en EAG. Les durées de rétention pour ce constituant correspondaient à celles des acétates monoinsaturés à 12 carbones, et les analyses sur colonne GC liées avec une phase liquide cristal ont montré un pic principal correspondant à l'acétate (E)-3-dodécénul et un pic secondaire, correspondant à l'isomère Z, et au niveau de 2,5% du pic principal. L'acétate dodécyl a aussi été décelé en quantités variables, approximativement 10% du pic principal.Dans la nature, des pièges contenant de l'acétate (E)-3-dodécényl, seul ou mélangé avec 1 ou 2% de l'isomère Z, ont capturé autant des mâles de S. solanivora que des pièges contenant des femelles vierges, et certaines indications montrent que l'addition de 5% de l'isomère Z réduit les captures.相似文献
18.
Electrophysiological and behavioural evidence for a fourth sex pheromone component in the turnip moth, Agrotis segetum 总被引:3,自引:0,他引:3
Abstract. In addition to the pheromone components (Z)-5-decenyl, (Z)-7-dodecenyl and (Z)-9-tetradecenyl acetate (Z5-10:OAc, Z7-12:OAc and Z9-14:OAc), it has previously been shown that the sex pheromone gland of the turnip moth, Agrotis segetum (Lepidoptera: Noctuidae Schiff) contains 10:OAc, 12:OAc, Z5-12:OAc, Z9-12:OAc, 11–12:OAc, Z5-14:OAc, Z7-14:OAc and Z11-16:OAc. To find out whether any of these additional compounds is involved in the sex pheromone communication in A. segetum, a comprehensive electro-physiological and behavioural investigation was conducted. Single-sensillum recordings on male antennae revealed three subtypes of sensilla among the previously so-called Z5-10:OAc sensilla. One subtype was identified having one receptor neurone (A) that responded to Z5-10:OAc with a large spike amplitude and another neurone (B) that responded to (Z)-5-decenol (Z5-10:OH) with a small spike amplitude. In another subtype the B neurone responded to Z5-12:OAc and sometimes also to 27-12:OAc and 10:OAc, in addition to responding to Z5-10:OH. In a third subtype the A neurone responded to all acetates identified from the female pheromone gland, whereas the small spike amplitude neurone was tuned to Z5-10:OH. A flight tunnel assay showed that blends composed of nine, eight or seven compounds were equivalent to the previously identified three-component pheromone blend in eliciting male behavioural responses. In field trapping tests, blends of eleven, nine or seven compounds did, however, catch significantly more moths than the three-component blend. Further assays showed that only 25- 12:OAc could significantly increase the catch numbers when added to the three-component blend, and thus qualified as a fourth pheromone component in A. segerum. The behavioural significance of additional female-produced acetates — for which males possess antennal receptors — is suggested, but may be impossible to confirm because of ‘diminishing returns’ when trying to refine a multicomponent pheromone further. 相似文献
19.
Junwei Zhu Sridhar Polavarapu Kye-Chung Park Carolyn Garvey Daniel Mahr Satoshi Nojima Wendell Roelofs Tom Baker 《Journal of Asia》2009,12(4):247-252
GC-EAD analyses of pheromone gland extracts of calling female Sparganothis sulfureana revealed at least 6 compounds that consistently elicited antennal responses from male antennae. In addition to the major pheromone compound, (E)-11-tetradecenyl acetate (E11–14:OAc), which was previously reported, the other compounds were found to be (E)-9-dodecenyl acetate (E9–12:OAc), (Z)-9-dodecenyl acetate (Z9–12:OAc), (Z)-9-tetradecenyl acetate (Z9–14:OAc), (Z)-11-tetradecenyl acetate (Z11–14:OAc), and (E)-11-tetradecenol (E11–14:OH). Tetradecyl acetate, hexadecyl acetate and hexadecenyl acetates were also present in the extracts, but elicited no EAG response from male antennae. Wind tunnel tests demonstrated that males from New Jersey responded equally well to a blend containing five pheromone components in relative to the pheromone glands of calling females. Different male-response profiles from field-trapping tests conducted in the states of Wisconsin and New Jersey were observed, respectively. Significantly higher numbers of male S. sulfureana were caught in New Jersey in traps baited with the binary blend of E11–14:OAc (30 μg) with 1% of Z11–14:OAc, but males from Wisconsin responded equally well to traps containing blends of E11–14:OAc with 0–10% of Z11–14:OAc. The addition of more than 10% of Z11–14:OAc to the primary pheromone compound reduced male captures significantly in both states. Male catches were doubled by adding E9–12:OAc and E11–14:OH to the most attractive binary blend in both states. The trapping test with caged live virgin female moths showed that males in Wisconsin preferred females from the local population than those from New Jersey. The differences in male responses observed may indicate the existence of pheromone polymorphism in this species. 相似文献
20.
Nami Miura Tatsuro Nakagawa Kazushige Touhara Yukio Ishikawa 《Insect biochemistry and molecular biology》2010,40(1):64-73
Mate-finding communication in many moths is mediated by sex pheromones produced by females. Since the differentiation of sex pheromones is often associated with speciation, it is intriguing to elucidate how the changes in sex pheromones are tracked by the pheromone recognition system of the males. Moths of the genus Ostrinia, which show distinct differentiation in female sex pheromones, are good models to study this. The present study was initiated with the aim of identifying ORs from Ostrinia scapulalis that respond to its own pheromone components, (E)-11- and (Z)-11-tetradecenyl acetates. We isolated six OR gene candidates (OscaOR3–8) from O. scapulalis. The same set of genes homologous to OscaOR3–8 were conserved in all (eight) Ostrinia species examined in addition to the previously reported OscaOR1 (tuned to (E)-11-tetradecenol) and the Or83b homologue OscaOR2. OscaOR3 not only responded to (E)-11- and (Z)-11-tetradecenyl acetates, but also to the pheromone components of the congeners, (Z)-9-, (E)-12-, and (Z)-12-tetradecenyl acetates. OscaOR4 responded with a relatively high specificity to (E)-11-tetradecenyl acetate. While OscaOR5 responded only marginally to a few pheromone components, OscaOR6–8 did not respond to any of the compounds tested. A few conserved ORs, including a unique one with very broad responsiveness, appear to be involved in the sex pheromone reception in O. scapulalis. The findings of the present study are discussed with reference to knowledge on electrophysiological response profiles of olfactory receptor neurons in Ostrinia moths. 相似文献