共查询到20条相似文献,搜索用时 15 毫秒
1.
E. Johnson J. Ringo H. Dowse 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(2):89-97
The heart of Drosophila melanogaster is a simple muscular tube with a posterior pulsatile portion and a thoracic-cranial vessel. The pacemaker, located caudally,
is myogenic. Its rate of firing is modulated by neurotransmitters. Serotonin, octopamine, norepineph-rine, dopamine, and acetylcholine
accelerate the heart, in that order of potency. Dihydroxyphenylalanine, γ-aminobutyric acid, glutamate, and glycine have no
effect. Generally, the regularity of the heartbeat is not adversely affected by treatment with any of these neurotransmitters.
We show here that amnesiac, a neurological mutation, and Dihydroxyphenylalanine decarboxylase
temperature sensitive, a mutation that interferes with synthesis of dopamine, norepinephrine, and serotonin, result in slower heart rate and reduced
regularity across a normal range of temperatures for these flies. Dopamine-N-acetyltransferase, which is on the catabolic route to dopamine, serotonin, and octopamine, has no effect. hypoactiveC reduces the rate of the heart, but its mechanism of action is unknown.
Accepted: 5 August 1996 相似文献
2.
Both octopamine and proctolin potentiate nerve-evoked skeletal muscle contractions in the horseshoe crab, Limulus. The threshold concentration for octopamine was 10?9 to 10?8M, while for proctolin it was 3 × 10?9M. Norepinephrine and dopamine produced effects similar to octopamine but at higher thresholds; tyramine and serotonin were ineffective. Octopamine caused significant increases in amplitudes of excitatory postsynaptic potentials (epsps) of muscle fibers, but had little effect on muscle fiber input resistance or membrane potential. Also, octopamine did not affect depolarization of muscle fibers and subsequent contraction due to the direct action of exogenously applied glutamate. These results suggest that octopamine potentiates nerve-evoked contractions primarily by facilitating release of neuromuscular transmitter. At concentrations above 10?7M, however, octopamine sometimes caused muscle spikes in response to motoneuron stimulation, a finding that suggests that octopamine may also have some postsynaptic action. Proctolin potentiated the muscle contractions evoked by glutamate but had little effect on glutamate-evoked muscle fiber depolarization, muscle fiber input resistance, or membrane potential. Thus, proctolin appears to act directly on skeletal muscle to enhance contractility. The proctolin-induced potentiations of contraction were sometimes accompanied by modest increases in epsp amplitude, so that unlike lobster skeletal and Limulus cardiac neuromuscular preparations, proctolin may have a secondary direct synaptic effect. Both octopamine and proctolin have been found in Limulus cardiac ganglion. This potential access to the hemolymph and the relatively low threshold concentrations needed for physiological action suggest that octopamine and proctolin could function as hormonal modulators of neuromuscular function in Limulus. 相似文献
3.
Photoperiodic time measurement regulating larval diapause in the pitcher-plant mosquito, Wyeomyia smithii, varies in a close relationship with latitude. The critical photoperiod mediating the maintenance and termination of diapause
is positively correlated with latitude (r
2 = 0.977) among six populations from southern (30–31° N), intermediate (40° N), and northern (46–49° N) latitudes in North
America. The developmental response to unnaturally short and to unnaturally long photoperiods declines with increasing latitude,
so that longer critical photoperiods are associated with a downward rather than a lateral shift in the photoperiodic response
curve. Exotic light and dark cycles of varying period (T) with a short (10 h) photophase and a scotophase ranging from 14
(T = 24) to 62 (T = 72) h, reveal two geographic patterns: a decline in perturbability of the photoperiodic clock with increasing
latitude, and no change with latitude in the 21-h period of rising and falling development with increasing T. These results
show (1) that there is a rhythmic component to photoperiodic time measurement in W. smithii, (2) that the period of this rhythm is about 21 h in all populations, and (3) that more northern populations show decreasing
responsiveness to photoperiod and increasing stability against perturbation by exotic period lengths (T > 24). Previous studies
on W.␣smithii indicate that this single temperate species of a tropical and subtropical genus has evolved from south to north. We therefore
conclude that the evolution of increasing critical photoperiod in W. smithii during its adaptive radiation into North America has more likely involved the amplitude and not the period of the underlying
circadian pacemaker.
Received: 22 July 1996 / Accepted: 30 September 1996 相似文献
4.
Dagmar Vogel Horst Bleckmann 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,180(6):671-681
The surface-feeding fish Aplocheilus lineatus uses its cephalic lateral line to detect water surface waves caused by prey insects. The ability of Aplocheilus to discriminate between surface waves with aid of the lateral line system was tested by go/no-go conditioning. Our results
show that Aplocheilus can distinguish between single-frequency surface wave stimuli with equal velocity or equal acceleration amplitudes which
differ only in frequency. Frequency difference limens were about 15%, i.e. fish distinguished a 20-Hz wave stimulus from a
23-Hz stimulus in 100% of the trials. Aplocheilus can also discriminate between pure sine-wave stimuli and sine waves which show abrupt frequency changes. In contrast, fish
were unable to distinguish amplitude-modulated wave stimuli (carrier frequency 20, 40 and 60 Hz, modulation frequency 10 and
20 Hz) from pure sine waves of the same frequency, even if amplitude modulation depth was 80%.
Accepted: 27 December 1996 相似文献
5.
N. Viswanathan Fred C. Davis 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,180(4):339-346
Pregnant Syrian hamsters with lesions of the suprachiasmatic nucleus (SCN) received single injections of melatonin or the
D1-dopamine receptor agonist, SKF 38393 on day 15 of gestation (1 day before birth). Pups were weaned on postnatal day 20
and their freerunning activity rhythms recorded for 3–4 weeks. The pups' phases on the day of weaning were significantly clustered
in both of the treatment groups, but the average phases differed by approximately 180°. The results demonstrate that a single
prenatal stimulus is sufficient to set the phases of the hamsters' rhythms and that the phase established depends on the stimulus.
Both c-fos mRNA and Fos protein were expressed in the fetal SCN after SKF 38393 injection but neither were expressed after melatonin injection. Simulations
showed that a single stimulus could produce the observed synchrony from a population of uniformally distributed phases if
the phase shifts were three to four times the magnitude of the adult hamster light phase response curve (PRC). A light pulse
PRC mimicked the effect of an SKF 38393 injection and a dark-pulse PRC mimicked the effects of a melatonin injection. Together
these results suggest that dopamine and melatonin either are, or mimic, maternal entraining signals that represent day and
night.
Accepted: 12 November 1996 相似文献
6.
George H. Renninger Leonard Kass Janice L. Pelletier Robert Schimmel 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1988,163(2):259-270
Efferent fibers from a central circadian clock innervate both photoreceptor cells and second-order neurons (eccentric cells) in the lateral compound eye ofLimulus, and release octopamine when activated. We have used intracellular microelectrodes to study the modulation of eccentric cell function by efferent optic-nerve activity, octopamine agonists, and a K+-channel blocker, TEA.
相似文献
1. | The dramatic increase in response to light observed in the eccentric cell during efferent activity originates in the photoreceptor cell; efferent activity causes only small changes in the encoding of photoreceptor responses as nerve impulses by the eccentric cell. In contrast, octopamine agonists and TEA produce large changes in the impulse encoder of the eccentric cell. |
2. | When lateral eyes are maintained in the dark, the rate of spontaneous impulse firing of eccentric cells increases in the presence of octopamine agonists, while spontaneous bump activity decreases. In contrast, endogenous efferent activity decreases both impulse rate and bump activity in the dark. |
3. | TEA reduces the effects of lateral inhibition between neighboring eccentric cells. |
4. | We suggest that the mechanisms for lateral inhibition and impulse generation are mediated by K+-channels that can be modulated by octopamine agonists. The distribution of efferent nerve terminals on the eccentric cells is such, however, that efferent optic-nerve activity can alter lateral inhibition, but is unlikely to produce large changes in the impulse encoder. |
7.
Thomas Waßmer Franziska Wollnik 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(4):270-279
Body temperature (T
b) of seven European hamsters maintained at constant ambient temperature (T
a = 8 °C) and constant photoperiod (LD 8:16) was recorded throughout the hibernating season using intraperitoneal temperature-sensitive
HF transmitters. The animals spent about 30% of the hibernation season in hypothermia and 70% in inter-bout normothermy. Three
types of hypothermia, namely deep hibernation bouts (DHBs), short hibernation bouts (SHBs), and short and shallow hibernation
bouts (SSHBs), were distinguished by differences in bout duration and minimal body temperature (T
m). A gradual development of SSHBs from the diel minimum of T
b during normothermy could be seen in individual hamsters, suggesting a stepwise decrease of the homeostatic setpoint of T
b regulation during the early hibernation season. Entry into hibernation followed a 24-h rhythm occurring at preferred times
of the day in all three types of hypothermia. DHBs and SHBs were initiated approximately 4 h before SSHBs, indicating a general
difference in the physiological initiation of SSHBs on the one hand and DHBs and SHBs on the other. Arousals from SHBs and
SSHBs also followed a 24-h rhythm, whereas spontaneous arousals from DHBs were widely scattered across day and night. Statistical
analyses of bout length and the interval between arousals revealed evidence for a free-running circadian rhythm underlying
the timing of arousals. The results clearly demonstrate that entries into hypothermia are linked to the light/dark-cycle.
However, the role of the circadian system in the timing of arousals from DHBs remains unclear.
Accepted: 11 December 1996 相似文献
8.
The effect of light environment, leaf area, and stored carbohydrates on inflorescence production by a rain forest understory palm 总被引:2,自引:0,他引:2
Saul A. Cunningham 《Oecologia》1997,111(1):36-44
Variation in flowering by long-lived plants may be correlated with current resource availability. If, however, there are
trade-offs between current and future reproduction, or between reproduction and storage or growth, then understanding variation
requires a whole-plant, longer-term perspective. Inflorescence production by Calyptrogyne ghiesbreghtiana Linden ex. H. Wendl., an understory palm, was studied over 3 years. Annual inflorescence production varied greatly and was
correlated with variation in plant size and light environment. There was no trade-off between past inflorescence production
and the frequency of future inflorescence production. On the contrary, individuals that produced more inflorescences than
predicted from their size and light environment tended to continue to do so in subsequent years also. I manipulated the resource
environment of a subset of plants by removal of leaves and/or reproductive spikes. Leaf removal suppressed inflorescence production
for the following 2 years, but spike removal had no effect. One year after leaf removal stored reserves were, on average,
back to pre-treatment levels. There was, however, a negative effect of recent inflorescence production on storage. Plants
with higher levels of storage had higher inflorescence production in the next 75 days. In C. ghiesbreghtiana the resource cost of reproduction is apparent in short-term variation in stored reserves. In contrast, annual inflorescence
production does not follow a trade-off pattern between successive years, but consistently reflects both plant size and the
light environment.
Received: 20 October 1996 / Accepted: 25 January 1997 相似文献
9.
10.
V. M. King B. K. Follett 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,180(5):541-551
c-fos induction was investigated as a potential component in the avian photic entrainment pathway and as a possible means of locating
the central pacemaker in birds. In both quail (Coturnix coturnix japonica) and starlings (Sturnus vulgaris) exposure to 1 h of light induced Fos-lir in the visual suprachiasmatic nucleus but not in the medial suprachiasmatic nucleus. However, the degree of c-fos induction in the visual suprachiasmatic nucleus was similar at different circadian times despite the fact that the light
pulses caused differential phase shifts in the locomotor rhythm. For golden hamsters the same experiment resulted in significantly
different levels of Fos-lir in the suprachiasmatic nucleus, as well as different phase shifts. Starlings and hamsters were also entrained to T-cycles that caused a large daily phase shift (T = 21.5 h in starlings, T = 22.67 hours in hamsters), or no daily phase shift (T = free running period). No difference in the induced levels of Fos-lir in the visual suprachiasmatic nucleus region was observed between the two groups of starlings, but in hamsters there were
significantly different levels of Fos-lir in the suprachiasmatic nucleus between the two groups.
Accepted: 15 November 1996 相似文献
11.
12.
Non-consumptive effects of larval Salamandra on crustacean prey: can eggs detect predators? 总被引:3,自引:0,他引:3
Leon Blaustein 《Oecologia》1997,110(2):212-217
Predators affect prey populations not only by prey consumption but also in nonconsumptive ways including modifying prey behavior.
I tested the effects of fire salamander larvae (Salamandra infraimmaculata) on populations of co-occurring crustacean species in artificial outdoor pools. I also tested whether these effects were
due entirely to prey consumption by Salamandra larvae or alternatively to some nonconsumptive effect. The soil (containing crustacean eggs) added to the artificial pools
was collected from a dried-out temporary pool that is inhabited by Salamandra during the early part of the hydroperiod. I randomly assigned the pools to one of three treatments: control, free Salamandra, or caged Salamandra. Free salamander larvae could roam the entire pool and prey upon crustaceans. Caged salamander larvae were placed within
a cage with having 250-μm mesh windows. They could not prey upon the crustaceans but could, for example, influence them by
chemical cues. Densities of the three dominant crustacean species (Arctodiaptomus similis, Ceriodaphnia quadrangula and Cyzicus sp.) were drastically reduced in both salamander treatments compared to the control. Crustacean densities, however, were
not significantly different between the two salamander treatments. One plausible explanation is that crustacean eggs can detect
the presence of this predator via chemical cues and delay hatching.
Received: 4 March 1996 / Accepted, 23 October 1996 相似文献
13.
Light response characteristics of a morphologically diverse group of southern hemisphere conifers as measured by chlorophyll fluorescence 总被引:5,自引:0,他引:5
Unlike northern hemisphere conifer families, the southern family, Podocarpaceae, produces a great variety of foliage forms
ranging from functionally broad-, to needle-leaved. The production of broad photosynthetic surfaces in podocarps has been
linked qualitatively to low-light-environments, and we undertook to assess the validity of this assumption by measuring the
light response of a morphologically diverse group of podocarps. The light response, as apparent photochemical electron transport
rate (ETR), was measured by modulated fluorescence in ten species of this family and six associated species (including five
Cupressaceae and one functionally needle-leaved angiosperm) all grown under identical glasshouse conditions. In all species,
ETR was found to increase as light intensity increased, reaching a peak value (ETRmax) at saturating quantum flux (PPFDsat), and decreasing thereafter. ETRmax ranged from 217 μmol electrons · m−2 · s−1 at a PPFDsat of 1725 μmol photons · m−2 · s−1 in Actinostrobus acuminatus to an ETR of 60 μmol electrons · m−2 · s−1 at a PPFDsat of 745 μmol electrons · m−2 · s−1 in Podocarpus dispermis. Good correlations were observed between ETRmax and both PPFDsat and maximum assimilation rate measured by gas-exchange analysis. The effective quantum yield at light saturation remained
constant in all species with an average value of 0.278 ± 0.0035 determined for all 16 species. Differences in the shapes of
light response curves were related to differences in the response of non-photochemical quenching (q
n), with q
n saturating faster in species with low PPFDsat. Amongst the species of Podocarpaceae, the log of average shoot width was well correlated with PPFDsat, wider leaves saturating at lower light intensities. This suggests that broadly flattened shoots in the Podocarpaceae are
an adaptation to low light intensity.
Received: 15 April 1996 / Accepted: 30 September 1996 相似文献
14.
Water relations dynamics during simulated sunflecks at high (36°C) and medium (27°C) temperatures and high and low vapour
pressure deficits beween leaf and air (VPD) were studied on shade-grown Piper auritum H.B. & K. plants, a pioneer tree, common in gaps and clearings of tropical rain forests. The leaves of P. auritum wilt rapidly when exposed to high light. Exposure to high VPD and high light caused substantial and rapid dehydration of
leaves. Dehydration could be prevented under high humidity irrespective of temperature. Water stored in leaf cells served
as initial source for transpiration upon high light exposure. This effect increased with increasing VPD and temperature. The
pronounced decrease in leaf water content over time in high light caused a rapid decrease in leaf water potential (Ψl) and a concomitant increase in water potential gradient (ΔΨ/Δx) between trunk and leaf, yet the high leaf elasticity (small bulk elastic modulus, ε) allowed turgor maintenance under most
conditions. Under high VPD and high temperature, stomata remained open and ΔΨ/Δx frequently exceeded 0.95 MPa · m−1, the cavitation-inducing threshold (ΔΨ/Δx
cav) causing high rates of acoustic emissions from stems and leaf petioles and leading to concomitant losses in hydraulic conductance
per leaf area (k
l). At medium temperature (high VPD), stomatal closure contained xylem embolism by keeping ΔΨ/Δx at or below this threshold. We argue that wilting substantially contributes to creating a sufficient driving force for water
uptake from the soil, and reducing the VPD (through a decrease in radiation load and thus leaf temperature) to avoid excessive
dehydration.
Received: 3 March 1996 / Accepted: 10 November 1996 相似文献
15.
DeForest Mellon Jr. 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,180(5):553-565
The cellular substrates of antennular flicking behavior in the crayfish Procambarus clarkii were investigated. Flicking involves fast downward movements of the external filament of each biramous antennule (1st antenna),
and is mediated by phasic contractions of a short muscle, the external filament depressor. Phasic contractions of the external
filament depressor depend upon stereotyped impulse bursts in a single motorneuron (P1). These bursts have a characteristic
impulse frequency profile that is consistent upon successive occurrences. The temporal characteristics of the impulse burst
suggest that the central depolarizations generating each burst may be similar to driver potentials described for motor neurons
in crustacean cardiac ganglia. Responses of the external filament to odorants have a long latency and are characterized by
repetitive bursts and tonic activity in some external filament depressor fibers. Tonic activity in a slowly contracting muscle,
the antennular depressor muscle, is also evoked by chemical stimulation. Flicking is consistently evoked only by mechanical
or hydrodynamic stimulation of the cephalothorax, antennae and antennules. The sensitivity and short latency of the hydrodynamic
antennule-generated flick reflex is consistent with the sensitivity of rapidly conducting, hydrodynamically activated mechanoreceptor
neurons in both antennular filaments. I propose that antennular flicking, which has been shown to enhance the dynamic response
characteristics of olfactory receptor neurons on the external antennular filament, has evolved as a response to the turbulence
associated with fluid movement, within which chaotic odorant concentration fronts may be imbedded.
Accepted: 23 October 1996 相似文献
16.
We are developing budding yeast, Saccharomyces cerevisiae, as a genetic system for the study of tolerance to the trivalent aluminum cation (Al3+). We have isolated eight mutants that are more sensitive to Al3+ than the wild type. Each mutant represented a different complementation group. A number of the mutants were pleiotropic,
and showed defects in other stress responses, changes in tolerance to other metal cations, or abnormal morphology. Two mutants
also showed increased dependence on supplemental Mg2+ and Ca2+. One mutant with a relatively specific sensitivity to Al3+ was chosen for molecular complementation. Normal Al3+ tolerance was restored by expression of the MAP kinase gene SLT2. Strains carrying deletions of the SLT2 gene, or of the gene for the corresponding MAP kinase–kinase SLK1, showed sensitivity to Al3+. These results indicate that the SLT2 MAP kinase signal transduction pathway is required for yeast to sense and respond to Al3+ stress.
Received: 17 April 1996 / Accepted: 21 October 1996 相似文献
17.
C. Traeholt 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(3):177-184
Five water monitor lizards, Varanus salvator salvator, and four clouded monitor lizards, Varanus bengalensis nebulosus, were caught on Tioman island in Malaysia. A radio-thermistor transmitter was implanted into the buccal cavity of each animal,
and they were released into an enclosure measuring 5.5 × 6.5 metres. The lizards were observed for 9 and 8 days, respectively,
before and after the parietal eye was covered with aluminium foil. With uncovered parietal eye, both species showed a clear
diurnal rhythm, being active only during day time. After covering the parietal eye, the mean locomotor activity of five V. s. salvator decreased from 791 to 107 min · day–1 but remained unchanged around 850 min · day–1 for V. b. nebulosus. The mean duration of locomotor activity decreased in V. s. salvator and V. b. nebulosus after the parietal eye was covered, but V. b. nebulosus maintained its locomotor activity by increasing the number of locomotor bouts. The water monitor spent very little time on
thermoregulation. Its body temperature ranged between 26.3 and 28.4 °C, which decreased after the parietal eye was covered.
The clouded monitor thermoregulated around 28.8–36.0 °C, which remained unchanged after the parietal eye was covered. In both
species, there was a strong correlation between body temperature and ambient temperature. Behavioural abnormalities were recorded
among V. s. salvator with covered parietal eye. They were often observed to be active by night and often slept outside a burrow. The circadian
rhythm of V. b. nebulosus appeared unaffected by shielding of its parietal eye. Captivity combined with shielded parietal eye induced agonistic behaviour
in both species.
Accepted: 11 September 1996 相似文献
18.
19.
Y. K. Ip G. Q. Tan S. S. L. Kuah S. F. Chew 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(3):213-220
The capability of Phascolosoma arcuatum to detoxify sulfide in anaerobic conditions was examined. Sulfane sulfur, which underwent cold cyanolysis, was the major
excretory end product of sulfide detoxification during anoxia. Thiosulfate was not excreted into the external medium. Instead,
it was absorbed by P. arcuatum and its absorption was stimulated by the presence of sodium sulfide (Na2S) in the incubation medium. The effective formation and excretion of sulfane sulfur by P.␣arcuatum required the presence of both Na2S and sodium thiosulfate (Na2S2O3). Results obtained indicate that rhodanese might be involved in sulfide detoxification in this sipunculid. Rhodanese could
act as a catalyst in the transfer of sulfur atoms from thiosulfate to HS−. The body wall and the introvert were the main sites of sulfide detoxification. However, it is unlikely that epibiotic bacteria
associated with the outside surface of the worm were involved in the detoxification process. A time-course study on the contents
of thiosulfate and sulfane sulfur in the body wall of P. arcuatum incubated anaerobically in the presence of Na2S + Na2S2O3 verified that thiosulfate absorbed was utilized to detoxify sulfide to sulfane sulfur.
Accepted: 24 October 1996 相似文献
20.
A. T. Carter F. Beiche B. Hove-Jensen A. Narbad P. J. Barker L. M. Schweizer M. Schweizer 《Molecular & general genetics : MGG》1997,254(2):148-156
In Saccharomyces cerevisiae the metabolite phosphoribosyl-pyrophosphate (PRPP) is required for purine, pyrimidine, tryptophan and histidine biosynthesis.
Enzymes that can synthesize PRPP can be encoded by at least four genes. We have studied 5-phospho-ribosyl-1(α)-pyrophosphate
synthetases (PRS) genetically and biochemically. Each of the four genes, all of which are transcribed, has been disrupted
in haploid yeast strains of each mating type and although all disruptants are able to grow on complete medium, differences
in growth rate and enzyme activity suggest that disruption of PRS1 or PRS3 has a significant effect on cell metabolism, whereas disruption of PRS2 or PRS4 has little measurable effect. Using Western blot analysis with antisera raised against peptides derived from the non-homology
region (NHR) and the N-terminal half of the PRS1 gene product it has been shown that the NHR is not removed by protein splicing. However, the fact that disruption of this
gene causes the most dramatic decrease in cell growth rate and enzyme activity suggests that Prs1p may have a key structural or regulatory role in the production of PRPP in the cell.
Received: 15 July 1996 / Accepted: 24 October 1996 相似文献