兵豆萌发过程中初生根皮层细胞的细胞周期活动的动态分析

观察了兵豆 (LensculinarisMedic.)初生根原皮层组织的细胞周期在其种子萌发过程中时间和空间上的动态变化。免疫组织化学和细胞学证据表明 ,原皮层细胞分别在种子吸胀大约 13h和 17h开始DNA复制和细胞分裂。最早进行DNA复制和细胞分裂的细胞位于远基端 1mm附近 ,但这些分裂细胞的DNA复制是在种子成熟过程中完成的 ,而不是在萌发后。第一个细胞周期的激活样式表明 ,这些细胞并不同步激活 ,而是依次进入细胞周期 ,且进入的次序与自身在根尖中的相对位置有关。在兵豆初生根原皮层组织中 ,邻近位置上的细胞的细胞周期同步化程度较高。     

兵豆萌发过程中初生根皮层细胞的细胞周期活动的动态分析

观察了兵豆(Lens culinaris Medic.)初生根原皮层组织的细胞周期在其种子萌发过程中时间和空间上的动态变化.免疫组织化学和细胞学证据表明,原皮层细胞分别在种子吸胀大约13 h和17 h开始DNA复制和细胞分裂.最早进行DNA复制和细胞分裂的细胞位于远基端1 mm附近,但这些分裂细胞的DNA复制是在种子成熟过程中完成的,而不是在萌发后.第一个细胞周期的激活样式表明,这些细胞并不同步激活,而是依次进入细胞周期,且进入的次序与自身在根尖中的相对位置有关.在兵豆初生根原皮层组织中,邻近位置上的细胞的细胞周期同步化程度较高.     

利用4种血清标志物筛查唐氏综合征的新方法

AmericanJournalofObstetricsandGynecology 2 0 0 2年 187卷 1期 197～ 2 0 1页报道 :日本国北海道大学医学院妇产科学系的医学博士东正树等 ,最近推出了利用兵豆 (Lensculinaris)凝集素反应性甲胎蛋白(AFP L3)比例 ,结合传统的血清标志物 ,来检测胎儿的唐氏综合征 (先天愚型 ,先天痴呆 )的新方法。东正树等采集了 5 30例未患病孕妇和 31例妊娠 14～ 2 0周怀了唐氏综合征胎儿的孕妇的血清。用记分法评价了兵豆凝集素反应性甲胎蛋白、甲胎蛋白、人绒毛膜促性腺激素和未结合雌三醇 4种血清标志物的不同组配的检测效果 ,而不考虑与孕妇年…     

兵豆甘油-3-磷酸酰基转移酶基因的克隆和序列分析及蛋白质二级结构的预测

克隆了云南强抗冷性植物兵豆 (Lensculinaris)GPAT基因的编码区完整的cDNA片段 ,该片段长 1374bp ,编码 4 5 7个氨基酸残基。与豌豆 (Pisumsativum)、蚕豆 (Viciafaba)和长柔毛野豌豆 (Viciavillosa)相比较 (从剪切点起 ) ,氨基酸序列的同源性分别为 96 2 1%、95 6 6 %和 95 6 6 %。应用PSIPREDHFORMAT预测了这 4种植物GPAT酶的二级结构。     

大白鼠心电图的分析

有关大白鼠心电图的资料,已有一些学者进行过研究,但资料不够全面,结果也不一致,鉴于目前有关学科在实验设计中,倾向于选用小动物作为实验研究对象,且常将心电图作为观察指标之一。为此,我们对大白鼠进行了心电图的描记和分析。     

国产兵豆中凝集素的分离纯化与生化特性的研究

从国产兵豆中用葡聚糖凝胶柱层析法分离出一种对人红细胞有凝集作用的凝集素,当其浓度为5—7μg/ml时即可引起血凝。通过电泳分析,分子量、氨基酸组成、糖与金属离子含量的测定,对其纯度进行了鉴定。比较了5种糖对此种凝集素血凝的抑制作用,结果以甲基α-D-甘露糖苷抑制能力最强,D-半乳糖无抑制作用。在交叉亲和免疫电泳中,此种凝集素能对多数原发性肝癌患者血清甲胎蛋白糖基有坟强的结合作用。     

乔治·福雷斯特在中国采集的杜鹃花属植物

在历时二十八年的植物考察生涯中,乔治.福雷斯特曾7次来华,为西方园林和植物研究机构采集、引种了大量的动、植物材料,在他所采集的中国杜鹃花标本中,曾被有关研究者作为新种描述的名称多达400余个,至今仍有150多种为众多研究者所接受。福雷斯特的采集是杜鹃花属分类和区系研究中非常重要的素材。     

一例罕见的X-常染色体平衡易位[46,X,t(Xq;15q]伴发自然流产

有关夫妇一方为平衡易位携带者所致流 产、死胎及先天性多发畸形的情况,近年来多见 报道,并日益引起妇产科医师和医学遗传工作 者的重视。绝大多数平衡易位发生在常染色体 与常染色体之间,极少发生在X染色体与常染 色体之间。由于X染色体对性发育的特殊影 响,所以这种少见类型的染色体易位更引人重 视。迄今为止,国内有关资料中尚未见X一常 染色体易位类型的报道。我们在为一对不明原 因自然流产的夫妇作外周血染色体检查中发现 女方的染色体核型为46, X, t (Xq+; 15q )o 现报道如下,并对某些平衡易位中的染色体部 分失活现象进行讨论。     

遗传性肾炎的遗传学研究进展

遗传性肾炎(hereditary nephritis,HN)是一组与遗传有关,主要累及肾小球的肾脏疾病,常伴有其它器官的损伤.HN呈家族聚集性,可表现为常染色体显性遗传、常染色体隐性遗传和X连锁遗传,有些家系还表现为非孟德尔遗传和线粒体遗传.对HN主要疾病的临床表型、遗传学和动物模型的总结和对HN疾病的深入研究有可能找到疾病的致病突变,以及更好地了解疾病的分子机制.     

一例罕见的X—常染色体平衡易位[46,X,t(Xq~+;15q~-)]伴发自然流产

有关夫妇一方为平衡易位携带者所致流产、死胎及先天性多发畸形的情况,近年来多见报道,并日益引起妇产科医师和医学遗传工作者的重视。绝大多数平衡易位发生在常染色体与常染色体之间,极少发生在X染色体与常染     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.