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1.
A bacterium that converted daidzein to O-desmethylangolensin was isolated from the feces of healthy humans. It was an obligately anaerobic, nonsporeforming, nonmotile
and Gram-positive rod. The isolate used glucose, sucrose, raffinose, maltose, and fructose as carbon sources. It did not hydrolyze
gelatin, esculin, or starch. The strain was urease, acid phosphatase, and arginine dihydrolase positive. It was catalase,
oxidase, H 2S, and indole negative. The major products of glucose fermentation were butyrate and lactate. Its mol% G+C was 51.2. The major
cellular fatty acids were C 16:0 DMA, C 16:0, and C 16:0 aldehyde. The structural type of cell wall peptidoglycan was suggested to be A1γ. The isolate was susceptible to β-lactam,
cefem, and macrolide antibiotics and resistant to aminoglycoside and quinolone antibiotics. The bacterium was related to Eubacterium ramulus ATCC29099 T, Eubacterium rectale ATCC33656 T, and species of the genus Roseburia, but the highest 16S rRNA gene similarity to these described species was only 94.4%, consistent with its being classified
as a novel genus. Based on the above, the isolate, named strain SY8519, was identified as belonging to a novel genus in the
Clostridium rRNA cluster XIVa. 相似文献
2.
A Gram-negative, short ovoid- to coccus-shaped, aerobic, motile, non-spore-forming bacterium (designated strain JLT1679 T) was isolated from West Pacific. Cells have subpolar flagella, dividing by binary fission. Phylogenetic analyses based on
16S rRNA gene sequences showed that the strain belongs to branch of the evolutionary radiation occupied by the genus Paracoccus, family Rhodobacteraceae, order Rhodobacterales, class Alphaproteobacteria. The closest neighbours were Paracoccus
stylophorae KTW-16 T (97.1% similarity), Paracoccus caeni strain MJ17 T (96.5% similarity), Paracoccus homiensis DD-R11 T (96.0% similarity) and Paracoccus alcaliphilus JCM 7364 T (95.8% similarity). The predominant cellular fatty acids of strain JLT1679 T were summed feature 8 (18:1ω6c) (38.8%), C 18:0 (27.7%), C 16:0 (22.5%), and significant amounts of C 18:1 ω9c (5.1%), C 14:0 (3.8%) and C 18:1 ω7c 11-methyl (2.1%), were present. The predominant respiratory ubiquinone of strain JLT1679 T was Q-10 and the DNA G + C content of strain JLT1679 T was 59.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine
and sphingoglycolipid. The isolate was distinguishable from members of the genus Paracoccus on the basis of phenotypic and biochemical characteristics. It is evident from the genotypic, chemotaxonomic and phenotypic
data that strain JLT1679 T represents a novel species of the genus Paracoccus, for which the name Paracoccus oceanense sp. nov. is proposed. The type strain is JLT1679 T (= JCM 17768 T = CGMCC 1.10831 T). 相似文献
3.
Thermal stress is considered one of the main causes of mass scleractinian coral degradation; however, it is still unknown how corals can adapt to future global warming. In this study, 11 strains of coral-associated Flavobacteria were shown to produce zeaxanthin, a carotenoid antioxidant, which may help coral holobionts to alleviate thermal stress. In addition, a novel zeaxanthin-producing Flavobacterium, designated R38T, was identified using polyphasic taxonomy. Although strain R38T shared a maximum 16S rRNA gene sequence similarity of 93% with Mesoflavibacter aestuarii KYW614T, phylogenetic analyses based on whole genome and 16S rRNA gene sequences revealed that strain R38T forms a distinct branch in a robust cluster composed of strain R38T and Leptobacterium flavescens KCTC 22160T under the family Flavobacteriaceae. Strain R38T exhibited average nucleotide identities of 70.2% and 72.5% for M. aestuarii KYW614T and L. flavescens KCTC 22160T, respectively. The only detected respiratory quinone was menaquinone 6 (MK-6). The genomic DNA G?+?C content was 33.2 mol%. The major polar lipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified ninhydrin phospholipid, three unidentified ninhydrin-positive lipids, and three unidentified lipids. The major cellular fatty acids were iso???C15:?0, iso???C15:?0 ω6c, C16:2 DMA, and C13:1 ω3c. The distinct biochemical, chemotaxonomic, phylogenetic, and phylogenomic differences from validly published taxa suggest that strain R38T represents a new species of a new genus, for which Prasinibacter corallicola gen. nov., sp. nov. is proposed. The type strain R38T (=?MCCC 1K03889T?=?KCTC 72444T). 相似文献
4.
An actinomycete strain, designated M1T8B9 T, was isolated from cow dung in Suwon, Republic of Korea. The isolate was a Gram-positive, nonmotile, and non-spore-forming
bacterium. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the genus
Microbacterium, with its closest neighbors being Microbacterium soli DCY17 T (98.2%) and Microbacterium esteraromaticum DSM 8609 T (98.0%). The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, and one unknown glycolipid. Strain
M1T8B9 T contained the major fatty acids C 15:0 anteiso, C 16:0 iso, C
17:0 anteiso, and C 15:0 iso, and the cell-wall peptidoglycan was of type B2β. According to DNA-DNA hybridization studies, strain M1T8B9 T showed 42% and 26% relatedness with M. soli DCY17 T and M. esteraromaticum DSM 8609 T, respectively. On the basis of the data presented, strain M1T8B9 T is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium suwonense sp. nov. is proposed. The type strain is M1T8B9 T (=KACC 14058 T =NBRC 106310 T). 相似文献
5.
A moderate halophilic, Gram-negative, non-motile, rod-shape, and aerobe designated as strain Hwa T was isolated from traditional fermented Korean seafood, which presented as a single cell or paired cells. Optimal growth
occurred at 25°C in 10% (w/v) salts at pH 7.0–8.0; however, growth occurred in a temperature range of 10–32°C, a salts concentration
of 5–25% (w/v) and pH 5.0–10.0. Tests for oxidase and catalase were positive. The cells produced poly- β-hydroxybutyric acid, but not exopolysaccharide. Based on the 16S rRNA gene sequence, not only was there low similarity between
strain Hwa T and all other species (94.1% similarity with H. subglaciescola DSM 4683 T, 94.0% similarity with H. sulfidaeris Esulfide1 T, 93.6% similarity with H. cerina SP4 T and 93.0% similarity with H. halodurans DSM 5160 T), but the phylogenetic analysis revealed that the isolate may be classified as a novel species belonging to the genus Halomonas in the class Gammaproteobacteria. The predominant fatty acids of strain Hwa T were C 18:1
ω7 c, C 16:0, C 12:0 3-OH and C 16:1
ω7 c/C 15:0 iso 2-OH. The DNA G+C content was calculated as 61.7 mol%. Based on phenotypic, genotypic, and phylogenetic characteristics,
it is proposed that the strain designated as Hwa T be assigned to the genus Halomonas as Halomonas jeotgali sp. nov. (=KCTC 22487 T =JCM 15645 T). 相似文献
6.
An aerobic, non-motile, Gram-stain positive, cellulose-degrading actinobacterium, designated strain NEAU-GS84T, was isolated from a forest soil sample collected from Linchun Ridge Forest Park in Sanya, Hainan Province, China, and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics indicated that strain NEAU-GS84T could belong to the genus Herbidospora. The 16S rRNA gene sequence analysis indeed confirmed that strain NEAU-GS84T belonged to the genus Herbidospora and was most closely related to Herbidospora yilanensis JCM 18062T (99.2% 16S rRNA gene sequence similarity) and Herbidospora galbida NEAU-GS14T (99.0%). The cell wall of strain NEAU-GS84T contained meso-diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates mainly contained glucose, madurose and ribose. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamin, phosphoglycolipids, two phosphatidylinositol mannosides and one unidentified phospholipid. The predominant menaquinone was MK-10(H4). Major fatty acids were 10-methly C17:0, C17:0 and iso-C16:0. These chemotaxonomic data substantiated the affiliation of strain NEAU-GS84T to the genus Herbidospora. The DNA G+C content was 70.7 mol%. The genome size of strain NEAU-GS84T is about 8.37 Mb and contained 41 cellulose-binding domain synthesis genes, 13 β-glucosidase synthesis genes, 6 endoglucanase synthesis genes and 9 xylanase synthesis genes. Based on digital DNA–DNA hybridization and average nucleotide identity values, the new strain NEAU-GS84T could be differentiated from its closest relatives. Therefore, the strain represents a novel species of the genus Herbidospora, for which the name Herbidospora solisilvae sp. nov. is proposed. The type strain is NEAU-GS84T (=?CCTCC AA 2018041T?=?JCM 33460T). 相似文献
7.
A novel Gram-stain positive, aerobic, non-motile, spore-forming actinobacterium, designated YIM 75926 T, was isolated from a soil sample collected at soil forest in Yuanmo county of Yunnan province, south-west China. Its taxonomic
position was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the
novel strain YIM 75926 T belongs to the genus Pseudonocardia and was closely related to Pseudonocardia
halophobica DSM 43089 T (98.1% similarity). Strain YIM 75926 T had MK-8 (H 4) as the predominant menaquinone. The whole organism hydrolysates mainly consisted of meso-diaminopimelic acid, mannose, glucose, galactose and arabinose. The major cellular fatty acids were iso-C 16:0 (37.16%) and C 16:0 (12.43%). The DNA G+C content of strain YIM 75926 T was 70.6 mol%. The resultant phylogenetic trees further showed that strain YIM 75926 T belong to Pseudonocardia and had a distinct subclade within the evolutionary radiation of the genus Pseudonocardia. On the basis of its comparative analysis of phenotypic and genotypic characteristics, it is proposed that strain YIM 75926 T represent a novel species of the genus Pseudonocardia, named Pseudonocardia
yuanmoensis sp. nov. The type strain is YIM 75926 T (=CCTCC AA 2011017 T = JCM 18055 T). 相似文献
8.
During the investigation of exploring potential sources of novel species and natural bioactives, a novel actinomycete, designated strain HIT-DPA4T, was isolated from a soil sample, which was collected from Nanjing, Jiangsu Province, PR China and characterized using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities and the result of phylogenetic analysis, strain HIT-DPA4T was most closely related to Streptomyces cyaneus CGMCC 4.1671 T, and shared the highest sequence similarity of 98.76%. In addition, the cell walls of the species HIT-DPA4T contained LL-diaminopimelic acid as the diagnostic diamino acid and the whole-cell hydrolysates were identified as glucose and ribose, and the principal phospholipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and phosphatidylmonomethylethanolamine. MK-9(H6) and MK-9(H4) were predominant menaquinones; and C16:0, anteiso-C15:0 and C15:0 as major cellular fatty acids of the organism HIT-DPA4T. Gene Ontology database analysis and antiSMASH server predicted results displayed that strain HIT-DPA4T was a promising classification units, which has various types of functions and contains multiple biosynthetic gene clusters with the similarity more than 80%. Multilocus sequence analysis (MLSA) of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) illustrated that Streptomyces luteolifulvus formed a separate branch in the genus Streptomyces. However, a combination of low level of DNA-DNA relatedness and physiological properties indicated that strain HIT-DPA4T can be distinguished from its phylogenetically related species Streptomyces cyaneus CGMCC 4.1671 T. Moreover, gene synteny research could be further differed organism HIT-DPA4T from similarity species. Therefore, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces luteolifulvus sp. nov. is proposed. The type strain is HIT-DPA4T (=?CGMCC 4.7558 T?=?TISTR 2751 T).
相似文献
9.
An ultraviolet-radiation-resistant, Gram-positive, orange-pigmented, thermophilic and strictly aerobic cocci was isolated
from Saharan water hot spring in Tunisia. The newly isolated bacterium, designated HAN-23 T, was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Phylogenetic
analysis based on 16S rRNA gene sequences placed this strain within Deinococcus genus. However, strain HAN-23 T is different from recognized species of the genus Deinococcus, showing less than 94.0% similarity values to its closest relatives. The predominant cellular fatty acids determined by gas
chromatography were iso-C 15:0, iso-C 17:0 and iso C 17:1 ω9c. The major respiratory quinone was MK-8. The DNA G + C content was 66.9 mol%. DNA–DNA hybridization measurements revealed
low DNA relatedness (6%) between the novel isolate and its closest neighbor, the type strain Deinococcus geothermalis DSM 11300. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain HAN-23 T represents a novel species of the genus Deinococcus, for which the name Deinococcus sahariens sp. nov. is proposed, the type strain being HAN-23 T (=DSM 18496 T; LMG 23756 T). 相似文献
10.
A gram-negative, motile, coccoid- and amorphous-shaped, non-pigmented chemoheterotrophic bacterium, designated strain PZ-5 T, was isolated from sea water of Sagami Bay in Japan and subjected to a polyphasic taxonomic study. Phylogenetic analysis
based on 16S rRNA gene sequences revealed that the novel isolate could be affiliated with the class Gammaproteobacteria. Strain PZ-5 T showed below 93.9% similarity with validly published bacteria and demonstrated the highest sequence similarity to Dasania marina KOPRI 20902 T (93.9%). Strain PZ-5 T formed a monophyletic group with D. marina KOPRI 20902 T. The DNA G+C content of strain PZ-5 T was 49.8 mol%. The major isoprenoid quinone was Q-8 and predominant cellular fatty acids were C 15:0 ISO 20H (19%), C 16:1 ω7c (17.4%), C 17;1 ω8c (16.2%), C 11:0 3OH (7.5%), and C 15:1 ω8c (6.5%). Based on evidence from a polyphasic taxonomical study, it was concluded that the strain should be classified
as representing a new genus and species of the class Gammaproteobacteria, for which the name Oceanicoccus sagamiensis gen. nov., sp. nov., (type strain PZ-5 T =NBRC 107125 T =KCTC 23278 T) is proposed. 相似文献
11.
A novel aerobic, bacteriochlorophyll-containing bacteria strain JLT1202r T was isolated from Beibu Gulf in the South China Sea. Cells were gram-negative, non-motile, and short-ovoid to rod-shaped with two narrower poles. Strain JLT1202r T formed circular, opaque, wine-red colonies, and grew optimally at 3–4?% NaCl, pH 7.5–8.0 and 28–30?°C. The strain was catalase, oxidase, ONPG, gelatin, and Voges–Proskauer test positive. In vivo absorption spectrum of bacteriochlorophyll a presented two peaks at 800 and 877?nm. The predominant cellular fatty acid was C 18:1 ω7c and significant amounts of C 16:0, C 18:0, C 10:0 3-OH, C 16:0 2-OH, and 11-methyl C 18:1 ω7c were present. Strain JLT1202r T contained Q-10 as the major respiratory quinone and the genomic DNA G+C content was 76.3?mol%. Phylogenetic analysis based on 16S rRNA gene sequences of various species with validly published names showed that strain JLT1202r T fell within the genus Roseibacterium, family Rhodobacteraceae, sharing the highest similarity with Roseibacterium elongatum OCh 323 T (97.9?% similarity), followed by Dinoroseobacter shibae DFL 12 T (95.4?% similarity). The phylogenetic distance of pufM genes between strain JLT1202r T and R. elongatum OCh 323 T was 9.4?%, suggesting that strain JLT1202r T was distinct from the only strain of the genus Roseibacterium. Based on the variabilities of phylogenetic and phenotypic characteristics, strain JLT1202r T stands for a novel species of the genus Roseibacterium and the name R. beibuensis sp. nov. is proposed with JLT1202r T as the type strain (=JCM 18015 T?=?CGMCC 1.10994 T). 相似文献
12.
A novel actinomycete, designated strain KLBMP 4601 T, was isolated from the root of the medicinal plant Curcuma phaeocaulis collected from Sichuan Province, south-west China. The strain produced extensively branched substrate and aerial hyphae that carried straight to flexuous spore chains. Chemotaxonomic properties of this strain were consistent with those of members of the genus Streptomyces. The cell wall of strain KLBMP 4601 T contained ll-diaminopimelic acid as the characteristic diamino acid. The major menaquinone was MK-9(H 4), with minor amounts of MK-9(H 6), MK-9(H 8) and MK-10(H 2). The major fatty acids were C 16:0, iso-C 16:0, C 18:1ω9 c and C 16:1, iso G. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KLBMP 4601 T belongs to the genus Streptomyces and is most closely related to Streptomyces armeniacus JCM 3070 T (97.9 %), Streptomyces pharmamarensis PM267 T (97.6 %) and Streptomyces artemisiae YIM 63135 T (97.5 %). The 16S rRNA gene sequence similarity between strain KLBMP 4601 T and other members of this genus were lower than 97.5 %. DNA–DNA hybridization studies of strain KLBMP 4601 T with the three closest species showed relatedness values of 36.3 ± 4.2 %, 27.3 ± 0.6 %, and 30.9 ± 2.5 %, respectively. On the basis of chemotaxonomic, phenotypic and genotypic characteristics, it is evident that strain KLBMP 4601 T represents a novel species of the genus Streptomyces, for which the name Streptomyces phytohabitans sp. nov. is proposed. The type strain is KLBMP 4601 T (=KCTC 19892 T = NBRC 108772 T). 相似文献
13.
A novel Gram-negative, orange-pigmented bacterial strain JLT2008 T was isolated from the surface seawater of the Western Pacific and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JLT2008 T belonged to the genus Erythrobacter, sharing the highest similarity (96.6 %) with Erythrobacter gangjinensis K7-2 T and the lowest similarity (94.9 %) with Erythrobacter litoralis DSM 8509 T. Strain JLT2008 T did not contain bacteriochlorophyll a, and the predominant respiratory lipoquinone was ubiquinone-10. The major fatty acids were C 18:1 ω7c, C 16:0, C 16:1 ω7c/C 16:1 ω6c. The prominent polar lipids were sphingoglycolipid, phosphatidylethanolamine, and phosphatidylglycerol. The genomic G + C content was 60.1 mol %. Based on the polyphasic taxonomic data, a novel species within the genus Erythrobacter, and with the name Erythrobacter westpacificensis sp. nov., is proposed. The type strain is JLT2008 T (=CGMCC 1.10993 T = JCM 18014 T). 相似文献
14.
A red-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium which was motile by gliding, designated strain 1351 T, was isolated from the soil of Lengduo, Tibet in China and subjected to a polyphasic taxonomic analysis. The isolate grows
optimally at 30°C and pH 7. It grows with NaCl tolerated up to 1.5% (optimum, 0.5%). Phylogenetic analysis based on the 16S
rRNA gene sequence shows that strain 1351 T is closely related to members of the family ‘ Cytophagaceae’ closest sequence similarity was observed with similarity with Adhaeribacter terreus (91.8%). The major whole-cell fatty acids are summed feature 4 (containing anteiso-C 17:1 B and/or iso-C 17:1 I, 29.2%), summed feature 3 (containing C 16:1ω6 c and/or C 16:1ω7 c, 13.0%) and iso-C 15:0 (12.0%). The predominant menaquinone of strain 1351 T is menaquinone-7 (MK-7) and the G+C content of the DNA is 46.8 mol%. According to the phylogenetic evidence and phenotypic
data, strain 1351 T is considered to represent a new genus and species of the family ‘ Cytophagaceae’ for which the name Rufibacter tibetensis gen nov., sp. nov. is proposed. The type species is R. tibetensis and the type strain is 1351 T (=CCTCC AB 208084 T = NRRL B-51285 T). 相似文献
15.
A thermophilic, rod-shaped, motile, Gram-positive, spore-forming bacterium strain 70B T was isolated from a geothermally active underground mine in Japan. The temperature and pH range for growth was 50–81°C (optimum
71°C) and 6.2–9.8 (optimum pH 7–7.5), respectively. Growth occurred in the presence 0–2% NaCl (optimum 1% NaCl). Strain 70B T could utilize glucose, fructose, mannose, mannitol, pyruvate, cellobiose and tryptone as substrates. Thiosulfate was used
as electron acceptor. Major whole-cell fatty acids were iso-C 15:0, C 16:0 DMA (dimethyl acetal), C 16:0 and anteiso-C 15:0. The G+C mol% of the DNA was 44.2%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the closest relatives
of strain 70B T were Thermosediminibacter oceani DSM 16646 T (94% similarity) and Thermosediminibacter litoriperuensis DSM 16647 (93% similarity). The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain 70B T represents a novel species in a new genus, for which the name Thermovorax subterraneus gen. nov., sp. nov. is proposed. The type strain of Thermovorax subterraneus is 70B T (=DSM 21563 = JCM 15541). 相似文献
16.
A Gram-negative, non-motile, non-spore-forming bacterial strain designated IBFC2009 T was isolated from soil of a bamboo plantation. The strain could grow at 11°C∼39°C, pH 6.0–9.0, and in the presence of 0∼5%
NaCl. Based on 16S rRNA gene sequence analysis, Strain IBFC2009 T belonged to the genus Sphingobacterium and showed the highest sequence similarity of 94.6% ( S. composti T5-12 T) with the type strains within the genus. The major fatty acids were summed feature 3 (iso-C 15:0 2-OH and/or C 16:1
ω7 c, 34.4%), iso-C 15:0 (22.4%), C 16:0 3-OH (15.2%), and iso-C 17:0 3-OH (12.8%). The G+C content of the genomic DNA was 41.0 mol%. According to the phenotypic and genotypic characteristics,
Strain IBFC2009 T should represent a novel species of the genus Sphingobacterium, for which the name Sphingobacterium bambusae sp. nov. is proposed. The type strain is IBFC2009 T (=CCTCC AB 209162 T =KCTC 22814 T). 相似文献
17.
Two bacterial strains, BT325T and BT690, were isolated from soil samples collected in Korea. Both strains were Gram stain-negative, short rod-shaped, and formed light-pink colored colonies. The 16S rRNA sequence similarity of strains BT325T and BT690 shared a sequence similarity of 99.7%. Both strains shared the highest 16S rRNA gene similarity of 98.6% with Microvirga arabica SV2184PT, followed by Microvirga ossetica V5/3 M T (98.5% and 98.2%, respectively), Microvirga soli R491T (98.3% and 98.2%, respectively), Microvirga aerilata (98.2% and 98.08%, respectively), Microvirga makkahensis (98.08% and 97.8%, respectively). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BT325T and BT690 were positioned in a distinct lineage within the family Methylobacteriaceae (order Rhizobiales, class Alphaproteobacteria). The genome size of strain BT325T was 5,200,315 bp and the genomic DNA G?+?C content was 64.3 mol%. The sole respiratory quinone of strain BT325T was Q-10 and the predominant cellular fatty acids were summed feature 3 (C16:1 ω7c/C16:1 ω6c) and summed feature 8 (C18:1 ω7c/C18:1 ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Polyphasic taxonomic analysis of biochemical, chemotaxonomic, and phylogenetic analyses suggested that strains BT325T represents a novel bacterial species within the genus Microvirga, for which the name Microvirga splendida is proposed. The type strain of Microvirga splendida is BT325T (=?KCTC 72406 T?=?NBRC 114847 T). 相似文献
18.
A Gram-negative, non-motile, non-endospore-forming bacterial strain, designated DPSR-4 T, was isolated from a tidal flat sediment on the southern coast of Korea. Strain DPSR-4 T grew optimally at 25–30°C, at pH 7.0–7.5 and in the presence of 2% (w/v) NaCl. A Neighbour-Joining phylogenetic tree based
on 16S rRNA gene sequences revealed that strain DPSR-4 T clustered with Salinimonas
chungwhensis BH030046 T by a high bootstrap resampling value of 99.7%. Strain DPSR-4 T exhibited 96.2% 16S rRNA gene sequence similarity to that of S. chungwhensis BH030046 T and 93.7–96.6% sequence similarity to the sequences of type strains of Alteromonas species. Strain DPSR-4 T contained Q-8 as the predominant ubiquinone and iso-C 15:0 2-OH and/or C 16:1
ω7 c, C 16:0 and C 18:1
ω7 c as the major fatty acids. The major polar lipids detected in strain DPSR-4 T and S. chungwhensis KCTC 12239 T were phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content was 53.4 mol%.
Differential phenotypic properties and phylogenetic distinctiveness of strain DPSR-4 T demonstrated that this strain is distinguishable from the sole recognized species of the genus Salinimonas, S. chungwhensis. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DPSR-4 T is considered to represent a novel species of the genus Salinimonas, for which the name Salinimonas lutimaris sp. nov. is proposed. The type strain is DPSR-4 T (KCTC 23464 T, CCUG 60743 T). 相似文献
19.
A Gram-staining-negative, oxidase-positive, catalase-positive, non-motile, non-spore-forming, and rod-shaped bacterium, designated
BJQ-6 T, was isolated from activated sludge of a waste-water treatment plant in Jiangsu Province, China. Phylogenetic analysis based
on 16S rRNA gene sequences showed that strain BJQ-6 T belonged to the genus Rhodanobacter, and shared 98.7% similarity with Rhodanobacter lindaniclasticus LMG 18385 T and <97% similarities with other Rhodanobacter species. The major fatty acids were iso-C 15:0 (17.6%), iso-C 16:0 (19.3%), and Summed feature 9 (isoC 17:1
ω9c and/or C 16:0 10-methyl) (25.8%). The DNA G+C content of strain BJQ-6 T was 64.8 mol%. Based on the phenotypic and phylogenetic considerations, strain BJQ-6 T represents a novel species of the genus Rhodanobacter, for which the name Rhodanobacter xiangquanii sp. nov. is proposed. The type strain is BJQ-6 T (=CCTCC AB 2010106 T =KCTC 23100 T). 相似文献
20.
A Gram-negative, non-motile, rod shaped, and orange-pigmented chemoheterotrophic bacterium, strain MS-31 T was isolated from the marine sponge Hymeniacidon flavia, collected from near Jeju Island, Korea. The Strain MS-31 T was subjected to a polyphasic taxonomic study. The phylogenetic analysis based on the 16S rRNA gene sequences revealed that
the novel isolate could be affiliated within the genus Sphingomonas. The strain MS-31 T showed 95.6% of 16S rRNA gene sequence similarity with the most closely related species Sphingomonas koreensis JSS26 T. The DNA G+C content of the strain MS-31 T was 69.4 mol%. The major isoprenoid quinone was ubiqunone 10 and predominant cellular fatty acids were summed feature 7 (comprising
C 18:1 ω7c, C 18:1 Ω9t and/or C 18:1 ωl2t, 39.7%), C 16:0 (16.3%), C 14:0 2OH (15.9%) and summed feature 3 (comprising C 16:1 ω7c and/or C 15:0 iso 2OH, 11.7%). The polar lipids were sphingoglycolipid, phosphatidyletha-nolamine, phosphatidylglycerol, diphosphatidylglycerol
and unidentified glycolipid. Based on the evidence from the polyphasic taxonomic study, the strain should be classified as
a new species of the genus Sphingomonas. As a result, the name Sphingomonas jejuensis sp. nov. (type strain MS-31 T =KCTC 23321 T =NBRC 107775 T) is proposed. 相似文献
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