Ueber die Bemerkungen des Hrn. Dr. W.Wolfner in Nr. 3, pag. 96 dieser Zeitschrift

Ohne Zusammenfassung     

pag gene-like protein (ABP-25) of the Cynops embryo: regional distribution and gene expression during early embryogenesis

A maternal protein showing a unique distribution during early Cynops embryogenesis was screened by monoclonal antibody. The antigen protein, designated as ABP-25 (animal blastomere protein, molecular weight 25,000), was distributed uniformly in the uncleaved egg and concentrated into blastomeres of the animal half during cleavage. At the blastula stage, ABP-25 was definitely localized in cells of the animal half and a polarized distribution was observed within the cytoplasm. During gastrulation, immunohistochemical analysis indicated that the reactivity of the marginal zone (presumptive mesoderm) to the monoclonal antibody ABP-25 decreased after involution. At the end of gastrulation, a polarized distribution was still clearly observed in the ventral epidermis, but not in the neuroectoderm. Both Western and Northern blots indicated that the amount of antigen protein and the intensity of gene expresion were almost constant until the neurula stage. The deduced amino acid sequence of the ABP-25 cDNA showed a strong homology (84%) with that of the pag gene associated with cell proliferation.The nucleotide sequence data reported in this paper will appear in the GSDB, DDBJ, EMBL and NCBI nucleotide sequence databases with the accession number D37808     

Revisione delle specie: Actinomyces albus,A. chromogenus,A. odorifer,A. thermophylus,A. viridis,A. viridochromogenes,A. hominis,A. innominatus

Riassunto Lo studio comprende una revisione critico-sperimentale della specie Actinomyces albus, della quale vengono considerati come sinonimi circa 30 nomi speeifici, fra i quale A. chromogenus, A. odorifer, A. thermophylus p.p.; della specie é data una diagnosi ed una particolareggiata descrizione.Sono inoltre studiate le specie A. viridis, (= A. viridochromogenus) e A. innominatus, n. nomen. Quest'ultima é preceduta da una breve discussione sulla specie A. homini.

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Summary Twenty-six strains of Actinomyces albus are studied redescribed from morphological, cultural and biochemical standpoints. Many biological activities of A. chromogenus, A. odorifer and A. thermophilus are in common with other species of the same genus, so that they may be considered for sub-specific, (not specific) differentiation. A discussion on A. farcinicus, A. albidoflavus and A. aureus has been originated from mislabeling as A. albus; the group including the two last named species (flavus group) must be revised. A few strains classified A. farcinicus are in no doubt true A. albus, but this real specific entity remains to be revised from Nocard's strain. A. viridis, for the first time described by Lombardo-Pellegrino, has been redescribed three times as a new species under the same binomial, and the fourth as A. viridochromogenes. A. hominis Bostroem is an uncorrect determination for the species originally described by Waksmann and Curtiss, and it is renamed A. innominatus, the binomial A. (Streptothrix) hominis Auct. being a nomen ambiguum. In conclusion, 30 bionmial are appended in sinonimy to A. albus, including Cladothrix dichotoma Macé (1888) non Cohn, G. invulnerabilis Acosta et G. Rossi, C. odorifera Rullm. Actinomyces chromogenus Gasp., A. thermophilus Auct., p.p., A. (Streptothrix) Sanninii (Cif.) Westerd., A. Almquisti Duché, A. Gougeroti Duché, and so on.

     

Hybridization and recombination in the yeasts Pichia alni, P. bimundalis, P. finlandica, P. glucozyma, P. henricii, P. hostii, P. minuta var. nonfermentans, P. muscicola

A collection of genetic lines in 8 reproductively isolated Pichia species has been created. The above data have permitted realizing intraspecific hybridization and showing normal meiotic segregation of auxotrophic markers.