GENUS ANTROPHYUM KAULF.FROM CHINA AND NEIGHBORING REGIONS

ＧＥＮＵＳＡＮＴＲＯＰＨＹＵＭＫＡＵＬＦ．ＦＲＯＭＣＨＩＮＡＡＮＤＮＥＩＧＨＢＯＲＩＮＧＲＥＧＩＯＮＳＸ．Ｃ．ＺＨＡＮＧ（Ｔｈｅｈｅｒｂａｒｉｕｍ（ＰＥ）,ＩｎｓｔｉｔｕｔｅｏｆＢｏｔａｎｙ,ＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＳｃｉｅｎｃｅｓ,Ｂｅｉ...     

STUDIES ON THE PATTERN OF MEGASPOROGENESIS AND MICROTUBULAR CYTOSKELETON CHANGES IN CYMBIDIUM SINENSE

ＳＴＵＤＩＥＳＯＮＴＨＥＰＡＴＴＥＲＮＯＦＭＥＧＡＳＰＯＲＯＧＥＮＥＳＩＳＡＮＤＭＩＣＲＯＴＵＢＵＬＡＲＣＹＴＯＳＫＥＬＥＴＯＮＣＨＡＮＧＥＳＩＮＣＹＭＢＩＤＩＵＭＳＩＮＥＮＳＥ￥Ｓ．Ｙ．ＺｅｅＸ．Ｌ．Ｙｅ（１ＢｏｔａｎｙＤｅｐａｒｔｍｅｎｔ,Ｕｎｉ...     

《游蛇科锦蛇属（Elaphe Fitzinger）专著》［德］

《游蛇科锦蛇属（ＥｌａｐｈｅＦｉｔｚｉｎｇｅｒ）专著》［德］ＡＭＯＮＯＧＲＡＰＨＯＦＴＨＥＣＯＬＵＢＲＩＤＳＮＡＫＥＳＯＦＴＨＥＧＥＮＵＳＥＬＡＰＨＥＦＴＴＺＩＮＧＥＲＫｌａｕｓ．Ｄｉｅｔｅｒ＆ｈｏｂ著。德国印制,捷克ＫｏｅｌｔＺ勤ｉｅｎｎｄＣＤ刀ｂ...     

山茶属—新组──柱蕊茶组

山茶属—新组──柱蕊茶组闵天禄（中国科学院昆明植物研究所，昆明６５０２０４）ＳＥＣＴ．ＣＹＬＩＮＤＲＩＣＡ，ＯＮＥＮＥＷＳＥＣＴＩＯＮＯＦＧＥＮＵＳＣＡＭＥＬＬＩＡ（ＴＨＥＡＣＥＡＥ）￥ＭＩＮＧＴｉｅｎ－Ｌｕ（ＫｕｎｍｉｎｇＩｎｓｔｉｔｕｔｅｏｆＢｏ...     

中国锥属一新种

中国锥属一新种符国瑷（海南省林业局标本室,海口５７０００３）关键词锥属;波叶锥ＡＮＥＷＳＰＥＣＩＥＳＯＦＣＡＳＴＡＮＯＰＳＩＳＢＬ．ＦＲＯＭＣＨＩＮＡ￥ＦｕＧｎｏａｉ（ＦｏｒｅｓｔｒｙＢｕｒｅａｕｏｆＨａｉｎａｎ,Ｈａｉｋｏｕ５７０００３）Ｋｅｙｗｏ...     

香港新记载的三种蕨类植物

香港新记载的三种蕨类植物苏美灵（香港浸会学院生物系，香港九龙窝打老道２２４号）ＮＥＷＲＥＣＯＲＤＳＯＦＦＥＲＮＳＩＮＨＯＮＧＫＯＮＧ￥ＳＵＭｅｉ－Ｌｉｎｇ（Ｍ．Ｌ．Ｓｏ）（ＢｉｏｌｏｇｙＤｅｐａｒｔｍｅｎｔ，ＨｏｎｇＫｏｎｇＢａｐｔｉｓｔＣｏｌｌｅｇ...     

CLONING AND CHARACTERIZATION OF A NOVEL NITRIC OXIDE SYNTHASE(NOS) FROM MOLLUSCAN CENTRAL GANGLIA

ＣＬＯＮＩＮＧＡＮＤＣＨＡＲＡＣＴＥＲＩＺＡＴＩＯＮＯＦＡＮＯＶＥＬＮＩＴＲＩＣＯＸＩＤＥＳＹＮＴＨＡＳＥ（ＮＯＳ）ＦＲＯＭＭＯＬＬＵＳＣＡＮＣＥＮＴＲＡＬＧＡＮＧＬＩＡＨｕａｎｇＳｈｉｌｅ１ＨｕｂｅｒｔＨ．Ｋｅｒｓｃｈｂａｕｍ２ＥｄｗｉｎＥｎｇｅｌ...     

钻地风属植物摘录

钻地风属植物摘录卫兆芬（中国科学院华南植物研究所,广州５１０６５０）ＮＯＴＥＳＯＮＳＣＨＩＺＯＰＨＲＡＧＭＡＳＩＥＢ．ＥＴＺＵＣＣ．（ＳＡＸＩＦＲＡＧＡＣＥＡＥ）ＦＲＯＭＣＨＩＮＡ￥ＷｅｉＣｈａｏｆｅｎ（ＳｏｕｔｈＣｈｉｎａＩｎｓｔｉｔｕｔｅｏｆＢｏ...     

社鼠核型的研究

社鼠核型的研究ＫＡＲＹＯＴＹＰＥＳＯＦＮＩＶＩＶＥＮＴＥＲＣＯＮＦＵＣＩＡＮＵＳ（ＲＯＤＥＮＴＩＡ：ＭＵＲＩＤＡＥ）关键词核型Ｃ带Ｇ带ＡｇＮＯＲｓ社鼠ＫｅｙｗｏｒｄｓＫａｒｙｏｔｙｐｅ,Ｃｂａｎｄｉｎｇ,Ｇｂａｎｄｉｎｇ,ＡｇＮＯＲＳ,Ｎ...     

金虎尾科的新分类群

金虎尾科的新分类群陈书坤（中国科学院昆明植物研究所，昆明６５０２０４）ＮＥＷＴＡＸＡＯＦＭＡＬＰＩＧＨＩＡＣＥＡＥＦＲＯＭＣＨＩＮＡＣＨＥＮＳｈｕ－Ｋｕｎ（ＫｕｎｍｉｎｇＩｎｓｔｉｔｕｔｅｏｆＢｏｔａｎｙ，ＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＳｃｉｅｎｃ...     

Quantitative chromatics analysis for computer imaging of cytologic subtypes of lung cancer stained by Papanicolaou stain

OBJECTIVE: To determine the role of quantitative chromatics analysis in the classification of subtypes of lung cancer stained by Papanicolaou stain. STUDY DESIGN: By means of computer image analysis, 60 keratinized squamous carcinoma cells (KSCC), 88 nonkeratinized squamous carcinoma cells (NKSCC) and 150 adenocarcinoma cells (ACC) from lung cancer in sputum smears stained by Papanicolaou stain were analyzed and distinguished based on quantitative colorimetry. The features measured were the content of three primary colors, red (R), green (G) and blue (B) and the coefficients of R, G and B (r, g and b, respectively). Hue, saturation, brightness and gray level were also measured. A stepwise discriminant analysis was carried out. RESULTS: The values of R, G and B and r, g and b, hue and saturation in NKSCC and ACC were significantly different from those of KSCC, and the changes in the three primary colors were more sensitive than those in the gray level. Computer assessment based on three primary color coefficients, hue and saturation yielded accuracy of distinguishing KSCC from NKSCC and KSCC from ACC of 95.2% and 95%, respectively. CONCLUSION: Quantitative analyses of R, G and B and r, g, b and hue and saturation are valuable in distinguishing KSCC from NKSCC and ACC.     

Histological subtypes and characteristic structures of HPV-associated oropharyngeal carcinoma; study with Japanese cases

Background

Human papillomavirus-associated oropharyngeal carcinoma (HPV-OPC) is clinicopathologically distinct entity from the HPV-unassociated one (nHPV-OPC). This study aimed to determine the relationship between histological subtypes of OPC and HPV status for Japanese cases and to identify histological structures of HPV-OPC.

Methods

66 OPC cases were categorized into conventional squamous cell carcinoma (SCC) and the variants. Conventional SCC was subcategorized into keratinizing (KSCC), non-keratinizing (NKSCC), and hybrid SCC (HSCC). HPV status of all cases was determined using p16-immunohistochemistry and HPV-DNA ISH.

Results

Two histological subtypes, NKSCC and HSCC, tended to be HPV-OPC and KSCC tended to be nHPV-OPC with statistical significance. Two histological structures, abrupt keratinization, defined in the text, and comedo-necrosis among non-maturing tumor island, were observed for 58.1% and 38.7% of HPV-OPC, and tended to exist for HPV-OPC with statistical significance.

Conclusions

This study showed the association of NKSCC/HSCC with HPV-OPC in Japanese cases, and two histological structures, abrupt keratinization and comedo-necrosis among non-maturing island, were considered characteristic histological features of HPV-OPC.

Virtual slides

The virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/1816432541113073.

     

Dichromatic color language: "reds" and "greens" don't look alike but their colors do.

When protanopes or deuteranopes arrange the Farnsworth Dichotomous Test colors in order of similarity, they reveal their lack of red/green hue discriminations by alternating chips that the normal trichromat sees as reddish and greenish test colors. The dichromatic orderings follow a systematic variation in saturation of blue hues through neutral and into yellow hues as described by theory for each of the two types. Some dichromats who show the typical test behavior nevertheless use reddish and greenish hue terms appropriately when instructed to name the same test colors. Lightness cues are probably used by these dichromats in the naming task but ignored in the perceptual similarity task. Thus, unlike normal trichromats, who use similar names for perceptually similar colors, dichromats may use dissimilar names for perceptually similar colors. In this way they can achieve concordance with the normative language system despite its discordance with their impoverished color perceptions.     

姜黄属植物过氧化物酶同工酶的研究

用聚丙烯酰胺凝胶电泳分析姜黄属14种(28个样品)植物的过氧化物酶同工酶。结果表明属内种间有明显的酶谱差异,各种都有特征酶谱。根据酶谱特征及酶谱距离,结合根茎颜色,可把14个种分成三群:第一群,根茎黄色至深红色,有姜黄、毛姜黄、郁金、印尼莪术、C.petiolata和C.sP.(2);第二群,根茎灰白色,有广西莪术、大莪术和温郁金;第三群,根茎浅黄而间淡蓝色或深蓝色,有莪术、顶花莪术、细莪术、C.aeruginosa及C.zedoaria。研究结果还表明:1.国产莪术的酶谱与从美国引入的C.zedoaria和C.aeruginosa均不相同,而与引自新加坡的C.Phaeocaulis一致;2.不同形态的广西莪术具有完全一致的酶谱;3.温郁金与郁金的酶谱有差异。     

The use of p63 as an effective immunomarker in the diagnosis of pulmonary squamous cell carcinomas on de-stained bronchial lavage cytological smears

M. Uke, B. Rekhi, D. Ajit and N. A. Jambhekar
The use of p63 as an effective immunomarker in the diagnosis of pulmonary squamous cell carcinomas on de‐stained bronchial lavage cytological smears Objectives: A diagnosis in pulmonary onco‐cytopathology primarily necessitates distinguishing small cell carcinoma (SCLC) from non‐small cell carcinoma (NSCLC), which includes squamous cell carcinoma and adenocarcinoma. Lately, p63 antibody has been used for distinguishing squamous cell carcinoma from SCLC and adenocarcinoma. We present an analysis of p63 expression in cytological smears from 100 bronchial lavage specimens comprising 51 cases of SCLC and 49 cases of NSCLC. Methods: A single Papanicolaou‐stained conventional smear was de‐stained and re‐fixed with cold acetone and methanol for immunocytochemical staining with p63 antibody. Staining results were graded as 0 (nil), 1+ (focal), 2+ (moderate, diffuse) and 3+ (strong, diffuse). Results: Out of 100 cases, 21 were cytologically diagnosed as squamous cell carcinoma. Twenty of these showed 2+ or 3+ p63 positivity, whereas one, which was adenocarcinoma on histology, showed 1+ staining. Of seven cases cytologically diagnosed as adenocarcinoma, six showed no p63 staining, whereas one, which was squamous cell carcinoma on histology, showed 1+ staining. All 48 cases cytologically diagnosed as SCLC were confirmed as such on histology and showed no p63 staining. Four cases were cytologically designated as poorly differentiated carcinomas, of which three showed no p63 staining and one showed 3+ staining. The former three were found to be SCLC on histology while the latter was squamous cell carcinoma. The remaining 20 cases were cytologically designated as NSCLC. Of these, eight showed no p63 staining, whereas 10 showed 1+ and two showed 2+ staining. The former eight were adenocarcinoma on histology and the latter two were squamous cell carcinoma. The 10 cases that showed 1+ p63 staining were adenocarcinomas (n = 5), squamous cell carcinoma (n = 4) and NSCLC, not otherwise specified (n = 1). Positive staining was seen in normal basal cells, which acted as an internal control. Overall sensitivity of p63 for squamous cell carcinoma was 100% and specificity was 90.4%. Conclusions: p63 immunostaining on processed cytology smears can be used to help identify squamous cell carcinoma. Its diffuse expression was specific for squamous cell carcinoma while focal staining was also seen in adenocarcinoma.     

细胞周期调控因子的表达与肺癌预后关系的研究

应用细胞周期调控因子P^27kipl,Rb单克隆抗体和CDK4多克隆抗体,对54例肺鳞状细胞癌的纤支镜活镜标本和10例正常肺组织进行免疫组织化学染色研究。结果发现：54例肺鳞癌中,P^27kipl阳性表达率为928/54）51.9%。表达水平与患预后呈正相关。P^27kipl表达是影响肺癌患预后的主要因素。CDK4阳性表达率（26/54）为48.1%,Rb阳性表达率（33/54）为61.1%。表达水平与患预后呈正相关。结果表明：P^27kipl低表达可作为判断肺鳞癌患预后差的一个独立的有效标记指标,CDK4表达对鉴别良恶性病变有一定意义,Rb表达可作为判断肺鳞癌预后的有意义指标。     

肺癌细胞多向分化与异质性的研究

探讨肺癌细胞多向分化与异质性。用光镜、免疫组化方法观察了87例肺癌手术标本组织切片,30例细胞涂片。（1）标本取材块数与病理分型种类多少里显著性正相关。（r=0．407,P＜0．01）;（2）作为单一类型的小细胞癌、鳞癌、腺癌、大细胞癌及类癌仅占27．6％、20％鳞癌、16．7％腺癌、23．3％大细胞癌KER、VIM双表达。66．7％有鳞、腺、神经内分泌三向分化;（3）在混合类型肺癌中,细胞学与组织学分型符合率为56．7％。肺癌细胞类型有明显异质性,其发生分子基础尚待进一步研究。     

White Ibis integument color during the breeding season

ABSTRACT.   Many birds undergo bare part color changes during the breeding season. Most investigators have focused on color as a signal of individual quality. An alternative, but not exclusive, function of bare part color may be signaling readiness to breed, especially in colonial, asynchronous breeders. White Ibises ( Eudocimus albus ) are colonial waterbirds that show vivid bare part colors on their bills and legs during reproduction. We quantified bill and leg colors to describe color changes and their possible relationship to reproductive status during the breeding season of White Ibises in the Florida Everglades from 1998 to 2001. We also examined the correlation between bare part colors and circulating concentrations of sex steroids to understand the factors that regulate bare part colors. During the display stage, male and female ibises developed dark pink bills and scarlet legs. As the breeding season progressed, bills and legs faded and developed a muted pink hue. The bare part colors of female ibises were correlated with testosterone concentrations, but those of male ibises were not correlated with any hormones. A discriminant function analysis based on principal component scores (representing variation in saturation and hue) and the amount of black on the bill successfully classified ibis reproductive stage 94% of the time. The use of bare part colors to determine reproductive status may be useful for studying reproduction in colonially nesting birds, where access to breeding sites can be difficult and potential for researcher disturbance is high.     

KIAA0101 is overexpressed, and promotes growth and invasion in adrenal cancer

Background

KIAA0101 is a proliferating cell nuclear antigen-associated factor that is overexpressed in some human malignancies. Adrenocortical neoplasm is one of the most common human neoplasms for which the molecular causes are poorly understood. Moreover, it is difficult to distinguish between localized benign and malignant adrenocortical tumors. For these reasons, we studied the expression, function and possible mechanism of dysregulation of KIAA0101 in human adrenocortical neoplasm.

Methodology/Principal Findings

KIAA0101 mRNA and protein expression levels were determined in 112 adrenocortical tissue samples (21 normal adrenal cortex, 80 benign adrenocortical tumors, and 11 adrenocortical carcinoma (ACC). SiRNA knockdown was used to determine the functional role of KIAA0101 on cell proliferation, cell cycle, apoptosis, soft agar anchorage independent growth and invasion in the ACC cell line, NCI-H295R. In addition, we explored the mechanism of KIAA0101 dysregulation by examining the mutational status. KIAA0101 mRNA (9.7 fold) and protein expression were significantly higher in ACC (p<0.0001). KIAA0101 had sparse protein expression in only a few normal adrenal cortex samples, which was confined to adrenocortical progenitor cells. KIAA0101 expression levels were 84% accurate for distinguishing between ACC and normal and benign adrenocortical tumor samples. Knockdown of KIAA0101 gene expression significantly decreased anchorage independent growth by 80% and invasion by 60% (p = 0.001; p = 0.006). We found no mutations in KIAA0101 in ACC.

Conclusions/Significance

KIAA0101 is overexpressed in ACC. Our data supports that KIAA0101 is a marker of cellular proliferation, promotes growth and invasion, and is a good diagnostic marker for distinguishing benign from malignant adrenocortical neoplasm.