共查询到20条相似文献,搜索用时 31 毫秒
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Cloning of the GATA-binding protein that regulates endothelin-1 gene expression in endothelial cells. 总被引:33,自引:0,他引:33
M E Lee D H Temizer J A Clifford T Quertermous 《The Journal of biological chemistry》1991,266(24):16188-16192
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Norwitz ER Xu S Xu J Spiryda LB Park JS Jeong KH McGee EA Kaiser UB 《The Journal of biological chemistry》2002,277(40):37469-37478
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An NF kappa B-like factor is essential but not sufficient for cytokine induction of endothelial leukocyte adhesion molecule 1 (ELAM-1) gene transcription. 总被引:29,自引:2,他引:27
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J Whelan P Ghersa R Hooft van Huijsduijnen J Gray G Chandra F Talabot J F DeLamarter 《Nucleic acids research》1991,19(10):2645-2653
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Darne C Martinez A Lallemand D Morel L Jean C Saru JP Schmid HP Manin M 《The Journal of steroid biochemistry and molecular biology》2000,72(3-4):103-113
Vas deferens epithelial cell subcultures were used to study the sequential regulation of jun/fos proto-oncogene expression and AP1 activities during cell proliferation, polarization and androgen-induced expression of a terminal differentiation marker, i. e. the mvdp gene. Proliferation of epithelial cells is associated with a high expression in the nucleus of most Jun and Fos oncoproteins. After cell seeding on an extracellular matrix which allows polarization and expression of the mvdp gene in response to androgens, AP1 protein accumulation is greatly altered and consists in a loss of JunB, Fra1, FosB and a decrease in c-Fos, c-Jun and Fra2, while JunD remained at the same level. This was correlated with a drop in AP1 binding activity as evaluated by gel shift assay using either AP1 consensus sequence or AP1 binding sites of the mvdp gene promoter region, and in AP1 transactivating activity, as estimated by stable transfection experiments using an AP1 responsive promoter (TRE-TK-luc). Androgens did not significantly influence AP1 activities. On the contrary, stimulation of AP1 proteins by the tumor-promoting phorbol ester caused a decrease in androgen-induced mvdp mRNA accumulation, and this effect was reversed by staurosporine, a potent inhibitor of PKC. Our data suggest that a down-regulation of AP1 activities induced by epithelial cell differentiation is a prerequisite to androgen-induced mvdp gene expression. The high AP1 activities observed during proliferative state or induced in TPA-treated polarized cells, exert a repressive effect on androgen action. 相似文献
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