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1.
E. Dirlewanger P. G. Isaac S. Ranade M. Belajouza R. Cousin D. de Vienne 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,88(1):17-27
An F2 population of pea (Pisum sativum L.) consisting of 174 plants was analysed by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) techniques. Ascochyta pisi race C resistance, plant height, flowering earliness and number of nodes were measured in order to map the genes responsible for their variation. We have constructed a partial linkage map including 3 morphological character genes, 4 disease resistance genes, 56 RFLP loci, 4 microsatellite loci and 2 RAPD loci. Molecular markers linked to each resistance gene were found: Fusarium wilt (6 cM from Fw), powdery mildew (11 cM from er) and pea common Mosaic virus (15 cM from mo). QTLs (quantitative traits loci) for Ascochyta pisi race C resistance were mapped, with most of the variation explained by only three chromosomal regions. The QTL with the largest effect, on chromosome 4, was also mapped using a qualitative, Mendelian approach. Another QTL displayed a transgressive segregation, i.e. the parental line that was susceptible to Ascochyta blight had a resistance allele at this QTL. Analysis of correlations between developmental traits in terms of QTL effects and positions suggested a common genetic control of the number of nodes and earliness, and a loose relationship between these traits and height. 相似文献
2.
Inukai T Vales MI Hori K Sato K Hayes PM 《Molecular plant-microbe interactions : MPMI》2006,19(9):1034-1041
Isolates of Magnaporthe oryzae (the causal agent of rice blast disease) can infect a range of grass species, including barley. We report that barley Hordeum vulgare cv. Baronesse and an experimental line, BCD47, show a range of resistance reactions to infection with two rice blast isolates. The complete resistance of Baronesse to the isolate Ken 54-20 is controlled by a single dominant gene, designated RMo1. RMo1 mapped to the same linkage map position on chromosome 1H as the powdery mildew resistance locus Mla and an expressed sequence tag (k04320) that corresponds to the barley gene 711N16.16. A resistance quantitative trait locus (QTL), at which Baronesse contributed the resistance allele, to the isolate Ken 53-33 also mapped at the same position as RMo1. Synteny analysis revealed that a corresponding region on rice chromosome 5 includes the bacterial blight resistance gene xa5. These results indicate that a defined region on the short arm of barley chromosome 1H, including RMo1 and Mla, harbors genes conferring qualitative and quantitative resistance to multiple pathogens. The partial resistance of BCD47 to Ken53-33 is determined by alleles at three QTL, two of which coincide with the linkage map positions of the mildew resistance genes mlo and Mlf. 相似文献
3.
V. Martinez P. Dettleff P. Lopez G. Fernandez A. Jedlicki J. M. Yañez W. S. Davidson 《Animal genetics》2013,44(2):223-226
Relatively large rates of response to traits of economic importance have been observed in different selection experiments in salmon. Several QTL have been mapped in the salmon genome, explaining unprecedented levels of phenotypic variation. Owing to the relatively large selection intensity, individual loci may be indirectly selected, leaving molecular footprints of selection, together with increased inbreeding, as its likely relatives will share the selected loci. We used population differentiation and levels of linkage disequilibrium in chromosomes known to be harbouring QTL for body weight, infectious pancreatic necrosis resistance and infectious salmon anaemia resistance to assess the recent selection history at the genomic level in Atlantic salmon. The results clearly suggest that the marker SSA0343BSFU on chromosome 3 (body weight QTL) showed strong evidence of directional selection. It is intriguing that this marker is physically mapped to a region near the coding sequence of DVL2 , making it an ideal candidate gene to explain the rapid evolutionary response of this chromosome to selection for growth in Salmo salar. Weak evidence of diversifying selection was observed in the QTL associated with infectious pancreatic necrosis and infectious salmon anaemia resistance. Overall, this study showed that artificial selection has produced important changes in the Atlantic salmon genome, validating QTL in commercial salmon populations used for production purposes according to the recent selection history. 相似文献
4.
A strategy to identify positional candidate genes conferring Marek''s disease resistance by integrating DNA microarrays and genetic mapping 总被引:12,自引:0,他引:12
Marker-assisted selection (MAS) to enhance genetic resistance to Marek's disease (MD), a herpesvirus-induced T cell cancer in chicken, is an attractive alternative to augment control with vaccines. Our earlier studies indicate that there are many quantitative trait loci (QTL) containing one or more genes that confer genetic resistance to MD. Unfortunately, it is difficult to sufficiently resolve these QTL to identify the causative gene and generate tightly linked markers. One possible solution is to identify positional candidate genes by virtue of gene expression differences between MD resistant and susceptible chicken using deoxyribonucleic acid (DNA) microarrays followed by genetic mapping of the differentially-expressed genes. In this preliminary study, we show that DNA microarrays containing approximately 1200 genes or expressed sequence tags (ESTs) are able to reproducibly detect differences in gene expression between the inbred ADOL lines 63 (MD resistant) and 72 (MD susceptible) of uninfected and Marek's disease virus (MDV)-infected peripheral blood lymphocytes. Microarray data were validated by quantitative polymerase chain reaction (PCR) and found to be consistent with previous literature on gene induction or immune response. Integration of the microarrays with genetic mapping data was achieved with a sample of 15 genes. Twelve of these genes had mapped human orthologues. Seven genes were located on the chicken linkage map as predicted by the human-chicken comparative map, while two other genes defined a new conserved syntenic group. More importantly, one of the genes with differential expression is known to confer genetic resistance to MD while another gene is a prime positional candidate for a QTL. 相似文献
5.
Grimmer MK Trybush S Hanley S Francis SA Karp A Asher MJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(7):1151-1160
Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is an important sugar-beet disease worldwide and can result in severe losses of root yield and sugar content. We
have identified a major QTL for BNYVV resistance from a new source in a segregating population of 158 individuals. The QTL
explained an estimated 78% of the observed phenotypic variation and the gene conferring the partial resistance is referred
to as Rz4. AFLP was used in combination with bulked segregant analysis (BSA) to develop markers linked to the resistance phenotype.
AFLP marker analysis was extended to produce a linkage map that was resolved into nine linkage groups. These were anchored
to the nine sugar-beet chromosomes using previously published SNP markers. This represents the first anchored sugar-beet linkage
map to be published with non-anonymous markers. The final linkage map comprised 233 markers covering 497.2 cM, with an average
interval between markers of 2.1 cM. The Rz4 QTL and an Rz1 RAPD marker were mapped to chromosome III, the known location of the previously identified BNYVV resistance genes Rz1, Rz2 and Rz3. The availability to breeders of new resistance sources such as Rz4 increases the potential for breeding durable disease resistance. 相似文献
6.
Caroline A. Wallace Saira Ali Anne M. Glazier Penny J. Norsworthy Danilo C. Carlos James Scott Tom C. Freeman Lawrence W. Stanton Anne E. Kwitek Timothy J. Aitman 《Mammalian genome》2002,13(4):194-197
The spontaneously hypertensive rat (SHR) is a model of human essential hypertension. Increased blood pressure in SHR is associated
with other risk factors associated with cardiovascular disease, including insulin resistance and dyslipidemia. DNA microarray
studies identified over 200 differentially expressed genes and ESTs between SHR and normotensive control rats. These clones
represent candidate genes that may underlie previously detected QTLs in SHR. This study made use of the publication of two
whole-genome maps to identify positional QTL candidates. Radiation hybrid (RH) mapping was used to determine the chromosomal
locations of 70 rat genes and ESTs from this dataset. Most of the locations are novel, but in five cases we identified a definitive
map location for genes previously mapped by somatic cell hybrids and/or linkage analysis. Genes for which the mouse genome
map location was already determined mapped to syntenic segments in the rat genome map, except for two rat genes whose map
locations confirmed previous findings. Where synteny comparisons could be made only with the human, 74% of the genes mapped
in this study lay in a conserved syntenic segment. Chromosomal localisation of these mouse and human orthologs to syntenic
segments produces a high level of confidence in the data presented in this study. The data provide new map locations for rat
genes and will aid efforts to advance the rat genome map. The data may also be used to prioritize candidate QTL genes in SHR
and other rat strains on the basis of their map location. 相似文献
7.
Genes for resistance to white rust (Albugo candida) in oilseed Brassica rapa were mapped using a recombinant inbred (RI) population and a genetic linkage map consisting of 144 restriction fragment length polymorphism (RFLP) markers and 3 phenotypic markers. Young seedlings were evaluated by inoculating cotyledons with A. candida race 2 (AC2) and race 7 (AC7) and scoring the interaction phenotype (IP) on a 0-9 scale. The IP of each line was nearly identical for the two races and the population showed bimodal distributions, suggesting that a single major gene (or tightly linked genes) controlled resistance to the two races. The IP scores were converted to categorical resistant and susceptible scores, and these data were used to map a single Mendelian gene controlling resistance to both races on linkage group 4 where resistance to race 2 had been mapped previously. A quantitative trait loci (QTL) mapping approach using the IP scores detected the same major resistance locus for both races, plus a second minor QTL effect for AC2 on linkage group 2. These results indicate that either a dominant allele at a single locus (Acal) or two tightly linked loci control seedling resistance to both races of white rust in the biennial turnip rape cultivar Per. The map positions of white rust resistance genes in B. rapa and Brassica napus were compared and the results indicate where additional loci that have not been mapped may be located. Alignment of these maps to the physical map of the Arabidopsis genome identified regions to target for comparative fine mapping using this model organism. 相似文献
8.
Jieying Li Keith A. Boroevich Ben F. Koop William S. Davidson 《Marine biotechnology (New York, N.Y.)》2011,13(2):232-241
Infectious salmon anemia (ISA) has been described as the hoof and mouth disease of salmon farming. ISA is caused by a lethal
and highly communicable virus, which can have a major impact on salmon aquaculture, as demonstrated by an outbreak in Chile
in 2007. A quantitative trait locus (QTL) for ISA resistance has been mapped to three microsatellite markers on linkage group
(LG) 8 (Chr 15) on the Atlantic salmon genetic map. We identified bacterial artificial chromosome (BAC) clones and three fingerprint
contigs from the Atlantic salmon physical map that contains these markers. We made use of the extensive BAC end sequence database
to extend these contigs by chromosome walking and identified additional two markers in this region. The BAC end sequences
were used to search for conserved synteny between this segment of LG8 and the fish genomes that have been sequenced. An examination
of the genes in the syntenic segments of the tetraodon and medaka genomes identified candidates for association with ISA resistance
in Atlantic salmon based on differential expression profiles from ISA challenges or on the putative biological functions of
the proteins they encode. One gene in particular, HIV-EP2/MBP-2, caught our attention as it may influence the expression of
several genes that have been implicated in the response to infection by infectious salmon anemia virus (ISAV). Therefore,
we suggest that HIV-EP2/MBP-2 is a very strong candidate for the gene associated with the ISAV resistance QTL in Atlantic
salmon and is worthy of further study. 相似文献
9.
Defense responses triggered by dominant and recessive disease resistance ( R) genes are presumed to be regulated by different molecular mechanisms. In order to characterize the genes activated in defense responses against bacterial blight mediated by the recessive R gene xa13, two pathogen-induced subtraction cDNA libraries were constructed using the resistant rice line IRBB13—which carries xa13 —and its susceptible, near-isogenic, parental line IR24. Clustering analysis of expressed sequence tags (ESTs) identified 702 unique expressed sequences as being involved in the defense responses triggered by xa13; 16% of these are new rice ESTs. These sequences define 702 genes, putatively encoding a wide range of products, including defense-responsive genes commonly involved in different host-pathogen interactions, genes that have not previously been reported to be associated with pathogen-induced defense responses, and genes (38%) with no homology to previously described functional genes. In addition, R -like genes putatively encoding nucleotide-binding site/leucine rich repeat (NBS-LRR) and LRR receptor kinase proteins were observed to be induced in the disease resistance activated by xa13. A total of 568 defense-responsive ESTs were mapped to 588 loci on the rice molecular linkage map through bioinformatic analysis. About 48% of the mapped ESTs co-localized with quantitative trait loci (QTLs) for resistance to various rice diseases, including bacterial blight, rice blast, sheath blight and yellow mottle virus. Furthermore, some defense-responsive sequences were conserved at similar locations on different chromosomes. These results reveal the complexity of xa13 -mediated resistance. The information obtained in this study provides a large source of candidate genes for understanding the molecular bases of defense responses activated by recessive R genes and of quantitative disease resistance.Electronic Supplementary Material Supplementary material is available in the online version of this article at
The first two authors contributed equally to this workCommunicated by R. Hagemann 相似文献
10.
Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice 总被引:8,自引:1,他引:7
Nguyen TT Klueva N Chamareck V Aarti A Magpantay G Millena AC Pathan MS Nguyen HT 《Molecular genetics and genomics : MGG》2004,272(1):35-46
We have developed 85 new markers (50 RFLPs, 5 SSRs, 12 DD cDNAs, 9 ESTs, 8 HSP-encoding cDNAs and one BSA-derived AFLP marker) for saturation mapping of QTL regions for drought tolerance in rice, in our efforts to identify putative candidate genes. Thirteen of the markers were localized in the close vicinity of the targeted QTL regions. Fifteen of the additional markers mapped, respectively, inside one QTL region controlling osmotic adjustment on chromosome 3 ( oa3.1) and 14 regions that affect root traits on chromosomes 1, 2, 4, 5, 6, 7, 8, 9, 10 and 12. Differential display was used to identify more putative candidate genes and to saturate the QTL regions of the genetic map. Eleven of the isolated cDNA clones were found to be derived from drought-inducible genes. Two of them were unique and did not match any genes in the GenBank, while nine were highly similar to cDNAs encoding known proteins, including a DnaJ-related protein, a zinc-finger protein, a protease inhibitor, a glutathione-S-transferase, a DNA recombinase, and a protease. Twelve new cDNA fragments were mapped onto the genetic linkage map; seven of these mapped inside, or in close proximity to, the targeted QTL regions determining root thickness and osmotic adjustment capacity. The gene I12A1, which codes for a UDP-glucose 4-epimerase homolog, was identified as a putative target gene within the
prt7.1/brt7.1 QTL region, as it is involved in the cell wall biogenesis pathway and hence may be implicated in modulating the ability of rice roots to penetrate further into the substratum when exposed to drought conditions. RNAs encoding elongation factor 1, a DnaJ-related protein, and a homolog of wheat zinc-finger protein were more prominently induced in the leaves of IR62266 (the lowland rice parent of the mapping materials used) than in those of CT9993 (the upland rice parent) under drought conditions. Homologs of 18S ribosomal RNA, and mRNAs for a multiple-stress induced zinc-finger protein, a protease inhibitor, and a glutathione-S-transferase were expressed at significantly higher levels in CT9993 than in IR62266. Thus several genes involved in the regulation of DNA structure and mRNA translation were found to be drought-regulated, and may be implicated in drought resistance.Communicated by R. Hagemann 相似文献
11.
Candidate defense genes from rice,barley, and maize and their association with qualitative and quantitative resistance in rice 总被引:23,自引:0,他引:23
Ramalingam J Vera Cruz CM Kukreja K Chittoor JM Wu JL Lee SW Baraoidan M George ML Cohen MB Hulbert SH Leach JE Leung H 《Molecular plant-microbe interactions : MPMI》2003,16(1):14-24
12.
Shu-Biao Wu Iraj Tavassolian Gholamreza Rabiei Peter Hunt Michelle Wirthensohn John P. Gibson Christopher M. Ford Margaret Sedgley 《Molecular genetics and genomics : MGG》2009,282(3):273-281
Peach and almond have been considered as model species for the family Rosaceae and other woody plants. Consequently, mapping
and characterisation of genes in these species has important implications. High-resolution melting (HRM) analysis is a recent
development in the detection of SNPs and other markers, and proved to be an efficient and cost-effective approach. In this
study, we aimed to map genes corresponding to known proteins in other species using the HRM approach. Prunus unigenes were searched and compared with known proteins in the public databases. We developed single-nucleotide polymorphism
(SNP) markers, polymorphic in a mapping population produced from a cross between the cloned cultivars Nonpareil and Lauranne.
A total of 12 SNP-anchored putative genes were genotyped in the population using HRM, and mapped to an existing linkage map.
These genes were mapped on six linkage groups, and the predicted proteins were compared to putative orthologs in other species.
Amongst those genes, four were abiotic stress-responsive genes, which can provide a starting point for construction of an
abiotic resistance map. Two allergy and detoxification related genes, respectively, were also mapped and analysed. Most of
the investigated genes had high similarities to sequences from closely related species such as apricot, apple and other eudicots,
and these are putatively orthologous. In addition, it was shown that HRM can be an effective means of genotyping populations
for the purpose of constructing a linkage map. Our work provides basic genomic information for the 12 genes, which can be
used for further genetic and functional studies. 相似文献
13.
Jun TH Rouf Mian MA Michel AP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(1):13-22
The soybean aphid (Aphis glycines Matsumura) is the most damaging insect pest of soybean [Glycine max (L.) Merr.] in North America. New soybean aphid biotypes have been evolving quickly and at least three confirmed biotypes
have been reported in USA. These biotypes are capable of defeating most known aphid resistant soybean genes indicating the
need for identification of new genes. Plant Introduction (PI) 567301B was earlier identified to have antixenosis resistance
against biotype 1 and 2 of the soybean aphid. Two hundred and three F7:9 recombinant inbred lines (RILs) developed from a cross of soybean aphid susceptible cultivar Wyandot and resistant PI 567301B
were used for mapping aphid resistance genes using the quantitative trait loci (QTL) mapping approach. A subset of 94 RILs
and 516 polymorphic SNP makers were used to construct a genome-wide molecular linkage map. Two candidate QTL regions for aphid
resistance were identified on this linkage map. Fine mapping of the QTL regions was conducted with SSR markers using all 203
RILs. A major gene on chromosome 13 was mapped near the previously identified Rag2 gene. However, an earlier study revealed that the detached leaves of PI 567301B had no resistance against the soybean aphids
while the detached leaves of PI 243540 (source of Rag2) maintained aphid resistance. These results and the earlier finding that PI 243540 showed antibiosis resistance and PI 567301B
showed antixenosis type resistance, indicating that the aphid resistances in the two PIs are not controlled by the same gene.
Thus, we have mapped a new gene near the Rag2 locus for soybean aphid resistance that should be useful in breeding for new aphid-resistant soybean cultivars. Molecular
markers closely linked to this gene are available for marker-assisted breeding. Also, the minor locus found on chromosome
8 represents the first reported soybean aphid-resistant locus on this chromosome. 相似文献
14.
An integrated linkage map reveals candidate genes underlying adaptive variation in Chinook salmon (Oncorhynchus tshawytscha)
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G. J. McKinney L. W. Seeb W. A. Larson D. Gomez‐Uchida M. T. Limborg M. S. O. Brieuc M. V. Everett K. A. Naish R. K. Waples J. E. Seeb 《Molecular ecology resources》2016,16(3):769-783
Salmonids are an important cultural and ecological resource exhibiting near worldwide distribution between their native and introduced range. Previous research has generated linkage maps and genomic resources for several species as well as genome assemblies for two species. We first leveraged improvements in mapping and genotyping methods to create a dense linkage map for Chinook salmon Oncorhynchus tshawytscha by assembling family data from different sources. We successfully mapped 14 620 SNP loci including 2336 paralogs in subtelomeric regions. This improved map was then used as a foundation to integrate genomic resources for gene annotation and population genomic analyses. We anchored a total of 286 scaffolds from the Atlantic salmon genome to the linkage map to provide a framework for the placement 11 728 Chinook salmon ESTs. Previously identified thermotolerance QTL were found to colocalize with several candidate genes including HSP70, a gene known to be involved in thermal response, as well as its inhibitor. Multiple regions of the genome with elevated divergence between populations were also identified, and annotation of ESTs in these regions identified candidate genes for fitness related traits such as stress response, growth and behaviour. Collectively, these results demonstrate the utility of combining genomic resources with linkage maps to enhance evolutionary inferences. 相似文献
15.
Kouassi AB Bakari KA Kerlan MC Marie-Claire K Caromel B Bernard C Dantec JP Jean-Paul D Fouville D Didier F Manzanares-Dauleux M Maria MD Ellissèche D Daniel E Mugniéry D Didier M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(4):699-707
Meloidogyne fallax is an emerging pest in Europe and represents a threat for potato production. We report the mapping of genetic factors controlling
a quantitative resistance against M. fallax identified in the Solanum sparsipilum genotype 88S.329.15. When infected, this genotype develops a necrotic reaction at the feeding site of the juveniles and totally
prevents their development to the female stage. A “F1” diploid progeny consisting of 128 individuals was obtained using the
potato (S. tuberosum) dihaploid genotype BF15 H1 as female progenitor. Sixty-eight hybrid genotypes displayed necrosis at the feeding site of
the juveniles and 60 other genotypes showed no defence reaction. This suggested a monogenic control of the resistance. However,
when considering the number of nematode females developed in their roots, a continuous distribution was observed for both
“necrotic” and “non-necrotic” hybrid genotypes, indicating a polygenic control of the resistance. A linkage map of each parental
genotype was constructed using AFLP markers. The necrotic reaction (NR) was mapped as a qualitative trait on chromosome XII
of the resistant genotype 88S.329.15. Quantitative trait locus (QTL) analysis for the number of nematode females developed
per “F1” plant genotype was performed using the QTL cartographer software. No QTL was detected on the linkage map of the susceptible
parent. A QTL explaining 94.5% of the phenotypic variation was mapped on chromosome XII of the resistant progenitor. This
QTL, named MfaXIIspl, was mapped in a genomic region collinear to the map position of the Mi-3 gene conferring resistance to Meloidogyne incognita in tomato. It corresponds to the NR locus. 相似文献
16.
Theodor Dingermann Elfriede Amon Keith L. Williams Dennis L. Welker 《Molecular & general genetics : MGG》1987,207(1):176-187
Summary Different wild-type isolates of Dictyostelium discoideum exhibit extensive polymorphism in the length of restriction fragments carrying tRNA genes. These size differences were used to study the organisation of two tRNA gene families which encode a tRNAVal(GUU) and a tRNAVal(GUA) gene. The method used involved a combination of classitics. The tRNA genes were mapped to specific linkage groups (chromosomes) by correlating the presence of polymorphic DNA bands that hybridized with the tRNA gene probes with the presence of genetic markers for those linkage groups. These analyses established that both of the tRNA gene families are dispersed among sites on several of the chromosomes. Information of nine tRNAVal(GUU) genes from the wild-type isolate NC4 was obtained: three map to linkage group I (C, E, F,), two map to linkage group II (D, I), one maps to linkage group IV (G), one, which corresponds to the cloned gene, maps to either linkage group III or VI (B), and two map to one of linkage groups III, VI or VIII (A, H). Six tRNAVal(GUA) genes from the NC4 isolate were mapped; one to linkage group I (D), two to linkage group III, VI or VII (B, C) and three to linkage group VII or III (A, E, F). 相似文献
17.
Nancy H. C. J. Roosens Glenda Willems Cécile Godé Adeline Courseaux Pierre Saumitou-Laprade 《Plant and Soil》2008,306(1-2):105-116
Arabidopsis halleri is a species that has undergone natural selection for zinc (Zn) tolerance. Isolation of the quantitative trait loci (QTL)
associated with this trait holds great promise for the identification of the main genes responsible for this adaptation. Using
a segregating progeny produced by an interspecific cross, we previously constructed a genetic linkage map of A. halleri × A. lyrata petraea and mapped the three main QTL that confer Zn tolerance in A. halleri (Willems et al.). The goal of the present study is to compare the genetic linkage map of A. halleri × A. l. petraea to the annotated A. thaliana genome sequence to generate a tool for A. halleri genomic approaches. To achieve this aim, we constructed a genetic linkage map with 81 markers anchored on A. thaliana, including 23 genes known to be involved in metal homeostasis. First, this provided an extensive overview of the chromosomal
rearrangements that have occurred since the divergence between A. thaliana and its closest relative A. halleri. Second, on the basis of the syntenic relationships assessed experimentally through this work, we transferred the QTL confidence
intervals for Zn tolerance to the A. thaliana physical map, allowing access to all the genes localized in the corresponding regions. Third, we validated from the 23 genes
involved in metal homeostasis the three ones localized in the QTL regions that can be considered the best candidates for conferring
Zn tolerance.
Nancy H. C. J. Roosens and Glenda Willems contributed equally to this paper. 相似文献
18.
Decroocq V Foulongne M Lambert P Gall OL Mantin C Pascal T Schurdi-Levraud V Kervella J 《Molecular genetics and genomics : MGG》2005,272(6):680-689
Plum pox virus (PPV), the causative agent of sharka disease in Prunoideae, is one of the most serious problems affecting stone fruit production in Europe and America. Resistance to PPV was previously described in a Prunus davidiana clone, P1908, and introduced into peach (Prunus persica) genotypes. Genetic resistance to PPV displays a complex pattern of quantitative inheritance. An analysis of quantitative trait loci (QTLs) for resistance was performed on an F1 interspecific peach population obtained from a cross between the susceptible nectarine cultivar Summergrand and P. davidiana. The hybrids were graft-inoculated with PPV in duplicate following a classical procedure. The incidence of infection was evaluated four times, over two vegetative cycles, by symptom observation and enzyme-linked immunoadsorbent assays (ELISA). Restriction of systemic downward movement of the PPV virus was also evaluated by testing the susceptible rootstocks. Using both analysis of variance and non-parametric tests, six genomic regions involved in PPV resistance were detected. Depending on the scoring data considered, between 22 and 51% of the phenotypic variance could be explained by the quantitative model. One QTL, located in the distal region of linkage group 1, maps in a genomic region that is syntenic to the location of a resistance gene previously identified in the apricot cv. Goldrich. Some QTLs appeared to be temporally specific, reflecting the environmental dependence of PPV-resistance scoring. Candidate gene fragments were amplified by PCR, isolated and mapped on the peach interspecific linkage map. We report here the co-localization of three analogues of virus resistance genes with two distinct genomic regions linked to PPV resistance in P. davidiana.Electronic Supplementary Material Supplementary material is available for this article at 相似文献
19.
Jerzy M. Behnke Fuad A. Iraqi John M. Mugambi Simon Clifford Sonal Nagda Derek Wakelin Stephen J. Kemp R. Leyden Baker John P. Gibson 《Mammalian genome》2006,17(6):584-597
Fine mapping of quantitative trait loci (QTL) associated with resistance to the gastrointestinal parasite Heligmosomoides polygyrus was achieved on F6/F7 offspring (1076 mice) from resistant (SWR) and susceptible (CBA) mouse strains by selective genotyping (top and bottom 20%
selected on total worm count in week 6). Fecal egg counts were recorded at weeks 2, 4, and 6, and the average was also analyzed.
Blood packed cell volume in weeks 3 and 6 and five immunological traits (mucosal mast cell protease 1, granuloma score, IgG1
against adult worm, IgG1, and IgE to L4 antigen) were also recorded. On Chromosome 1 single-trait analyses identified a QTL
with effects on eight traits located at about 24 cM on the F2 mouse genome database (MGD) linkage map, with a 95% confidence interval (CI) of 20-32 cM established from a multitrait analysis.
On Chromosome 17 a QTL with effects on nine traits was located at about 18 cM on the MGD map (CI 17.9-18.4 cM). Strong candidate
genes for the QTL position on Chromosome 1 include genes known to be involved in regulating immune responses and on Chromosome
17 genes within the MHC, notably the Class II molecules and tumor necrosis factor. 相似文献
20.
Gion Jean-Marc Rech Philippe Grima-Pettenati Jacqueline Verhaegen Daniel Plomion Christophe 《Molecular breeding : new strategies in plant improvement》2000,6(5):441-449
We used the single-strand conformation polymorphism (SSCP) technique to map eight genes on Eucalyptus urophylla and Eucalyptus grandis linkage maps. These included four genes involved in the common phenylpropanoid pathway (caffeic acid 3-0-methyltransferase, caffeoyl CoA 3-O-methyltransferase, 4-coumarate CoA ligase and phenylalanine ammonia-lyase), two genes involved in the `lignin specific' pathway (cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase), and two symbiosis regulated genes (EgHypar and EgTubA1). A novel source of variation which affects the SSCP pattern, i.e. the presence or absence of electrophoresis buffer upon loading the samples into the polyacrylamide gel, was found. The placement of these genes on the Eucalyptus maps was carried out using an interspecific hybrid mapping population. This will further facilitate the identification or exclusion of `positional' candidate genes for characterizing quantitative trait loci (QTL) for wood quality and vegetative propagation related traits. 相似文献