不同水分管理方式下水稻的水分利用效率与产量

采用温室微区试验研究常规水作、裸地旱作、覆膜旱作和覆草旱作等土壤水分管理下水稻生长及其对水分的利用状况。结果表明,几种旱作水稻的需水量为349-473mm,常规水和水稻需水量为762.5mm。旱作水稻的水分籽粒和干物质生产效率为0.899-1.273g·kg^-1和1.655-2.321g·kg^-1之间,而相同条件下常规水作水稻水分的籽粒和干物质生产效率为0.766g·kg^-1和1.459g·kg^-1左右。覆草旱作水稻可以获得相当于常规水作水稻90％的经济产量。     

Application of infrared spectroscopy (attenuated total reflection) for monitoring enzymatic activity on substrate films

Infrared film analysis, a method based on infrared spectroscopy in the mode of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), is demonstrated as a novel analytical method for monitoring enzymatic activity on surface-attached substrate films in the mid infrared range (400-4000 cm(-1)). The ATR-FTIR technique is sensitive to molecules within a distance of approximately 1 microm from the ATR sampling unit surface (a 7 cm(2) hydrophobic ZnSe crystal). Applying a 0.2-0.3 microm thick film on the ATR unit surface, any chemical changes within this film as well as at the interface can be continuously monitored, even having an aqueous phase on top of the film. Infrared film analysis is considered especially useful for studying detergent enzymes, which act on surface bound films consisting of food component like vegetable oils (triacylglycerols) and carbohydrates (e.g. starch). Experimental data are presented for hydrolysis of a triacylglycerol film (triolein) by use of a triacylglycerol lipase (cutinase), and starch film degradation by use of an alpha-amylase.     

A Rapid,Sensitive, Seedling-Based Bioassay for the Determination of Toxicity of Solid and Liquid Substrates and Plant Tolerance

We have developed a rapid, sensitive, seedling-based bioassay that can be used to evaluate the relative toxicity of solid and liquid media and to evaluate plant tolerance to toxic compounds. Using this method, it is possible to use percent germination, root and shoot growth as parameters. For this bioassay, solid substrate such as soil or acid-washed sand is sandwiched between two layers of microfiber filter paper inside a perforated Petri dish that bathes within a larger reservoir. The addition of distilled water or liquid media to the reservoir causes the liquid to pass through the perforations in the inner dish, through the solid substrate to form a film at the top of the filter paper that covers the substrate. Seeds are placed into the film of solution for germination and subsequent growth. Unlike germinating seeds in soil, the early germination of seeds can easily be observed. A distinction can then be made between seeds that do not germinate and those that do germinate but subsequently cease growth, giving the toxicity bioassay additional sensitivity. Root growth may be monitored as a time-course or measured at the end of the experiment. In addition, visual symptoms of stress such as swelling or coloration can be observed throughout the experiment. While shoot growth is often found to poorly reflect toxicity of media, we have found a good response in this parameter. The bioassay has been used to test relative toxicities of metal-contaminated soils, metal solutions, hydrocarbon-contaminated soils and soil leachates and airborne hydrocarbons accumulated in plant tissues.     

Autoclavable ultrahigh-efficiency filter for use with flexible film germfree animal isolators

An autoclavable ultrahigh-efficiency filter unit, which accommodated 99.98% efficient respirator filter cartridges, was built for use with flexible film germfree animal isolators.     

Vibrational circular dichroism of carbohydrate films formed from aqueous solutions

Vibrational circular dichroism (VCD) spectra in the entire 2000-900 cm(-1) region have been recorded, for the first time, for films of carbohydrates prepared from aqueous solutions. Eight different carbohydrates, alpha-D-glucopyranosyl-(1-->4)-D-glucose, cyclomaltohexaose, alpha-D-glucopyranosyl alpha-D-glucopyranoside, beta-D-glucopyranosyl-(1-->6)-D-glucose, beta-D-glucopyranosyl-(1-->4)-D-glucose, D-glucose, and both enantiomers of 6-deoxygalactose and of allose, were investigated. The VCD spectra obtained for films are found to be identical to the corresponding spectra obtained for aqueous solutions of carbohydrates. These measurements demonstrate several advantages of significant importance. The strong infrared absorption of water has prevented, in the past, the pursuit for routine applications of VCD in determining the structures of carbohydrates in aqueous solutions. This limitation is not present for film studies because water solvent is removed in the process of preparing the films. Also, strong infrared absorption of water at 1650 cm(-1) requires the use of very short-pathlength (6 microm) cells for measurements on aqueous solutions. This requirement and concomitant inconveniences (such as laborious assembling of a demountable liquid cell or purchasing an expensive variable pathlength liquid cell) have been eliminated for film measurements. The removal of interfering water absorption in film studies resulted in higher light throughput and better signal-to-noise ratios for VCD measurements. Another point of significance is that the amount of carbohydrate sample required for VCD measurements on films is approximately one to two orders of magnitude smaller than that required for corresponding VCD measurements on aqueous solutions. Since carbohydrate samples can now be studied as films, VCD spectroscopy becomes much more broadly applicable for carbohydrates than previously believed. The present work, in combination with other film measurements in our laboratory, indicate that VCD studies on films can be used more generally, providing a convenient and powerful approach for probing structural information for biologically important compounds.     

Focal plane array infrared imaging: a new way to analyse leaf tissue

* Here, a new approach to macromolecular imaging of leaf tissue using a multichannel focal plane array (FPA) infrared detector was compared with the proven method of infrared mapping with a synchrotron source, using transverse sections of leaves from a species of Eucalyptus. * A new histological method was developed, ideally suited to infrared spectroscopic analysis of leaf tissue. Spatial resolution and the signal-to-noise ratio of the FPA imaging and synchrotron mapping methods were compared. * An area of tissue 350 microm(2) required approx. 8 h to map using the synchrotron technique and approx. 2 min to image using the FPA. The two methods produced similar infrared images, which differentiated all tissue types in the leaves according to their macromolecular chemistry. * The synchrotron and FPA methods produced similar results, with the synchrotron method having superior signal-to-noise ratio and potentially better spatial resolution, whereas the FPA method had the advantage in terms of data acquisition time, expense and ease of use. FPA imaging offers a convenient, laboratory-based approach to microscopic chemical imaging of leaves.     

地表处理方式对日光温室辣椒水分利用效率及土壤硝态氮、速效磷分布的影响

研究了不同地表处理方式对日光温室辣椒水分利用效率及土壤氮磷分布的影响.结果表明:地表覆盖秸秆 地膜处理的辣椒产量水分利用效率和经济水分利用效率最高,分别达33·04kg·m-3和50·22元·m-3;其次是地表覆盖地膜处理,分别达18·81kg·m-3和28·57元·m-3.不同地表处理方式对0～20cm土壤的硝态氮含量有显著影响,地表覆盖秸秆和覆盖秸秆 地膜处理,分别为31·98mg·kg-1和31·96mg·kg-1,小于对照处理(50·33mg·kg-1);地表覆盖地膜和使用保水剂处理的硝态氮含量较低.与对照相比,各处理辣椒对氮肥的利用均有所增加,耕层硝态氮积累减少.在0～20cm耕层内,地表覆盖地膜处理的速效磷含量最低,为0·72mg·kg-1,其次是地表覆盖秸秆 地膜处理,为0·92mg·kg-1.地表覆盖秸秆 地膜和地表覆盖地膜处理增加了当季作物对肥料的利用率,减少了肥料的损失,提高了产量.     

不同耕作和覆盖方式对紫色丘陵区坡耕地水土及养分流失的影响

采用随机区组试验,研究了四川紫色丘陵区坡耕地不同耕作和覆盖方式对玉米生育期中水土及养分流失的影响。结果表明:秸秆覆盖对减少水土流失和增加玉米产量的效果均优于地膜覆盖。秸秆覆盖能显著减少地表径流(73.9%—86.2%),但增加了壤中流(15.4%—156.4%);使径流总量降低32.5%—66.6%,并极显著降低土壤侵蚀总量达96.4%—98.1%。地膜覆盖虽能在一定程度上减少壤中流和径流总深,但差异未达到显著水平。土壤N平均损失量达37.4kg/hm2,其中70.1%经由壤中流流失。秸秆覆盖虽然增加了一定的壤中流N损失,但能减少N流失总量达12.8%—65.1%。土壤P素损失量相对较小,仅为9.32kg/hm2,并主要随侵蚀泥沙迁移,占流失总量的92.1%。土壤K损失量达183.3kg/hm2,其流失载体也主要是侵蚀泥沙,占96.5%。因此,两种覆盖方式均能显著控制土壤P和K的损失。无论是秸秆还是地膜覆盖,与顺坡垄作相比,横坡垄作均能减少地表径流、地下径流、土壤侵蚀量及氮、磷、钾素总流失量,同时还能提高玉米产量。从简便、增产和防治面源污染的角度考虑,紫色土区坡耕地最适宜的种植方式为平作+秸秆覆盖。     

Interaction of plastic mulch,pesticides and fungi in the control of soil-borne nematodes

Summary Three different mulching materials — hay, black polyethylene film, and translucent polyethylene film — produced different soil temperatures and thus influenced the microflora of the soil differently.Rhizoctonia solani Kuehn increased in cool or moderate temperatures under hay or black film but not in warmer soil beneath translucent film. Populations of a meadow nematode,Pratylenchus penetrans Cobb were reduced approximately two-thirds under the black film. Under this film conditions apparently favor biological control ofP. penetrans. Over several months this control was destroyed if a fungicide, pentachloronitrobenzene, and a nematocide, dibromochloropropane, were added. Evidently, the fungicide inhibited organisms predaceous onP. penetrans at the same time it inhibitedR. solani.     

The use of iron oxide-impregnated filter paper for the extraction of available phosphorus from Taiwan soils

Procedures were established for the preparation of iron oxide-impregnated filter paper and for its use in the determination of plant-available soil phosphorus.The amounts of phosphate extracted by the procedure from 39 soils of the major agricultural areas of Taiwan were well correlated with those extracted by young wheat seedlings or other chemical extractants commonly in use for soil testing. It was shown that the iron oxide-impregnated filter paper method is applicable to a wide range of soils in Taiwan and that the results are comparable with those obtained with the anion-exchange resin and Olsen methods.     

天津市彩色红外相片在研究植被与大气污染关系中的应用

利用彩色红外相片来研究城市植被与大气污染的关系,这是遥感技术应用于环境动态研究中的一个重要课题。在研究过程中,使用了中国科学院遥感所提供的天津市1／5000和1／10000比例尺彩色红外相片。我们首先将显示在相片上的植被与大气污染的遥感信息进行了解译,然后取样分析该地植物体中污染物质的含量,最后再到实地验证。结果表明,彩色红外相片记录了当时植被与大气污染的真实状况,具有丰富的信息,植被信息特征能够反映大气污染的基本情况。同时还可发现一些抗性强、吸毒能力大的绿化效应好的树种和绿化结构,可为城市建设、城市环境质量评价提供科学依据。     

地膜覆盖对土壤水温和春小麦产量形成的影响

通过大田试验研究了地膜覆盖对土壤水温状况及春小麦产量形成的影响．结果表明,地膜覆盖对土壤的增温作用在春小麦生育期内呈“U”型变化,地膜覆盖可以通过防止蒸发和提升土壤深层水分至作物可利用层来增加土壤中有效水含量,利于作物利用．地膜覆盖的增温保墒作用利于作物前期生长和水分利用,在生育后期覆膜,作物根系发育受到抑制,作物蒸散量和水分利用效率下降,影响产量的形成．对照(CK)、播前灌水(W)、全程覆膜(M)、播前灌水 覆膜30d(WM30)、播前灌水 覆膜60d（WM60)及播前灌水十全程覆膜(WMw)6个处理的产量分别为2554、2424、2750、3138、3305、3123kg·hm^-2,最佳覆膜时间在40—60d．     

黄土旱塬不同覆盖对春玉米产量及土壤环境影响

对黄土旱塬用不同覆盖对春玉米产量及土壤环境影响的研究表明,春玉米采用不同覆盖技术,均有显著的增产效果,能显著增加土壤贮水量,使土壤上层长期保持湿润状态,提高土壤温度(除秸秆覆盖外),降低昼夜温差,避免降雨直接冲击地面。保持良好的土壤结构,渗水地膜覆盖与秸秆覆盖、常规地膜覆盖和不覆盖相比,其产量分别增长6．4％、23．6％和29．1％,水分利用效率分别为21．5、20．2、17．4和16．7kg·mm^-1·hm^-2,秸秆覆盖还能提高土壤有机质,增加土壤肥力渗水地膜和常规地膜具有相同的增温效果,当气温达到35℃以上时,渗水地膜还具有降低极端温度的调节功能,但是,覆盖易造成有机质大量矿化和NO3^--N的淋失。     

覆膜对旱地麦田土壤水分及氮素平衡的影响

通过大田试验研究了平膜穴播和垄膜沟播等覆膜方式对晋南旱地麦田土壤水分、氮素平衡及产量的影响,以期在当地确立一套适宜的科学覆膜方式,为晋南旱塬地区乃至我国旱作小麦的高产优质提供理论依据。结果表明,垄膜沟播和平膜穴播处理的冬小麦增产效果显著,且以平膜穴播处理的效果最优,较测控施肥处理的籽粒产量和生物产量分别提高22.71%和25.45%。经过冬小麦一个生育期对土壤水分的吸收利用,两种覆膜处理的耗水量较不覆膜处理有较大的提高,而其水分利用率略低于不覆膜处理,但差异不显著。两种覆膜处理也能提高麦田的降水生产效率和休闲效率,较不覆膜处理分别提高9.46%—30.16%和9.95%—39.22%。覆膜有利于氮的矿化,并能促进小麦对氮素的吸收利用,同时也可以在一定程度上降低氮素在土壤中的残留,最终有利于小麦增产。     

Economical sequencing of DNA with terminators

We describe several improvements of chain-termination DNA sequencing procedure of Sanger et al. For template preparation we use 0.3 ml cultures of M13 clones, grown in standard 1,5 ml polypropylene tubes. The sequencing experiment differs from the previously described by the use of deoxyNTP, labelled with phosphorus-33 (a low energy isotope with a half-life of 25 days, commercially produced in the USSR), and by a "quasi-end labelling" reaction, preceding the DNA synthesis in the presence of dideoxyNTPs. The combination of the phosphorus-33 and the quasi-end labelling produces very sharp sequencing ladders, that equal or exceed in quality those obtained with sulphur-35, and only an overnight exposure with a conventional X-ray film is required. The use of plastic tubes for bacterial growth and the 60-well microchambers for carrying out sequencing reactions results in substantial saving of time and cost in routine "middle scale" sequencing (both types of plasticware are produced in the USSR).     

酞酸酯污染农田土壤生物修复研究进展

酞酸酯是目前世界上产量最大、应用面积最广的人工合成有机物,作为塑化剂被广泛应用于塑料制品中。近年来发现酞酸酯是一类典型的环境内分泌干扰物。随着生活中塑料制品日益增多,尤其是农用薄膜和有机肥的大量使用,农田土壤中酞酸酯污染日益加剧,酞酸酯污染土壤的修复逐渐引起国内外学者的广泛关注。生物修复具有价格低廉、效果良好和环境友好等特点,尤其适合于大面积污染农田土壤修复。从植物修复、微生物修复、植物微生物联合修复和动物修复等方面综述了国内外酞酸酯污染土壤生物修复的研究现状,并从高效修复植物筛选及机理探讨、实际污染土壤的降解菌修复研究、高效降解菌群的构建和作用机制等方面对该领域的研究进行了展望,以期为酞酸酯污染土壤的修复研究提供借鉴并拓展新的思路。     

Detection of mycoparasitism by infrared photomicrography

The fungusTrichoderma harzianum which parasitizes its hostRhizoctonia solani (AG 1–6) was observed under a light microscope and the interaction sites photomicrographed with infrared film. Bright regions indicating infrared irradiation were observed at the interaction sites, apparently due to the high parasitic activity occurring there. The possible use of infrared photomicrography in cell-cell interactions is discussed.     

A selective molecularly imprinted polymer‐carbon nanotube sensor for cotinine sensing

Conductive composite films comprised of single‐walled carbon nanotubes coated with molecularly imprinted poly‐4‐vinylphenol are produced and characterized using ultraviolet and infrared spectroscopies, confirming the successful molecular imprinting of the film with cotinine. The electrical resistance of the imprinted film changes significantly upon binding cotinine, by more than 30 kΩ, while the unimprinted film in comparison elicits little response. Additionally, once the cotinine template desorbs from the film, the resistance of the imprinted film returns to a value close to the pre‐adsorption baseline. Scanning electron microscopy is used to study the morphology of the film compared with the unimprinted control, and gas chromatography quantitatively confirms that the imprinted film selectively detects cotinine while discriminating against the structurally similar alkaloid, nicotine. Copyright © 2013 John Wiley & Sons, Ltd.     

False-positive coliform readings using membrane filter techniques for seawater

Seawater samples that had been analysed by membrane filter techniques produced positive coliform readings that were found to be false. Apparently, the culture media and the incubation temperatures used in these methods permit the growth of vibrios which are abundant in the seawater. The vibrio colonies isolated by membrane filter were oxidase-positive Gram-negative short rods that grew on TCBS and required NaCl.     

干旱胁迫下覆膜和接种微生物对玉米生长与水分利用效率的影响

针对西北干旱半干旱地区土壤贫瘠与水分缺乏的问题,利用微生物与作物形成互惠互利的共生关系,本研究设置两个水分梯度:干旱胁迫(供试土壤最大持水量的35%)和正常水分(供试土壤最大持水量的75%),两个覆膜方式:无覆膜(NM)和覆膜(FM),4个接种微生物水平:单接AM真菌(AM)、单接解磷细菌(PSB)、联合接种AM真菌与解磷细菌(AM+PSB)以及对照(CK),研究不同水分和覆膜条件下4个接种微生物对玉米生长特性、地上养分吸收与水分利用效率的影响.结果表明: 与正常水分处理相比,干旱胁迫能够显著提高接种AM真菌处理的侵染率,但正常水分处理下土壤根外菌丝密度、总球囊霉素(T-GRSP)与易提取球囊霉素(EE-GRSP)含量明显提高.干旱胁迫下,单接AM真菌处理的促生作用和菌根效应表现最好,能够提高玉米生物量、水分利用效率和土壤有机碳含量,促进土壤N、P、K的吸收与运输,从而增加玉米地上部分N、P、K吸收量;而正常水分下,联合接种AM+PSB处理表现要好于单接AM和PSB处理,且其与覆膜的互作效果最好.相关分析结果表明,玉米生物量、叶片SPAD值和地上部分N、P、K吸收量均与土壤根外菌丝密度呈显著正相关,玉米水分利用效率与其呈显著负相关.