Karyological notes on six beetle species from Armenia (Coleoptera: Tenebrionidae,Cerambycidae, Curculionidae)

Karyotypic details were studied in males of six beetle species from three families, viz. Tenebrionidae: Dailognatha pumila Bdy. (2n = 20, n(male) = 9 + Xy(p)), Pachyscelis musiva Ménétr. (2n = 18, n(male) = 8 + Xy(p)), Pimelia capito Kryn. (2n = 18, n(male) = 8 + Xy(p)); Cerambycidae: Agapanthia walteri Reitt. (2n = 20, n(male) = 9+Xy(p)), Agapanthia korostelevi Danilevsky (2n = 20, n(male) = 9 + Xy(p)); Curculionidae: Phyllobius caucasicus Stierl. (2n = 22, n(male) = 10 + Xy(p)). The chromosome number and sex determining system of all beetle species are described for the first time. Evolutionary trends in karyotypes of the studied beetle groups are briefly discussed.     

A new contribution to the cytogenetic knowledge of Alticinae (Coleoptera, Chrysomelidae)

Seven species of Alticinae, mostly from Spain, have been chromosomally surveyed from male meiotic or mitotic cells. The two Spanish species of Oedionychus, O. cinctus and O. limbatus, have shown a karyotype of 2n=16+X+Y, with a lower number of autosomes than in their congeneric Neotropical species, but sharing with them the giant distance-paired sex-chromosomes. The Uruguayan Macrohaltica transversa has a 11+X+y meioformula, which agrees with that found in four congeneric species and in most ones of the allied genus Altica. The meioformula of Hermaeophaga ruficollis 7+Xy, differs only slightly from that reported in another congeneric species. Moreover those of Longitarsus aeruginosus 11+Xy, L. australis 13+Xy, and Psylliodes obscuroaeneus 15+Xy(p,) are similar to some of those previously published for other species in both genera.     

Karyotype of blue honeysuckle species (Lonicera subset. Caeruleae,Caprifoliaceae

For the first time the karyotypes of diploid (2n = 2x = 18) and tetraploid (2n = 4x = 36) species of Lonicera from the Caeruleae subsection: L. altaica Pall., L. boczkarnikowii Plekh. (L. regeliana Boczkarn.), L. edulis Turcz. ex Freyn (2x, 4x), L. emphyllocalyx Maxim., L. iliensis Pojark., L. kamtschatica Pojark., L. pallasii Ledeb., L. stenantha Pojark., L. turczaninowii Pojark., L. villosa (2x, 4x) (Michx.) Muhl. are described. The species karyotypes from 23 natural populations have shown the considerable generic resemblance that expressed in the similar chromosome morphology and variation range of their length from 1 to 3 microns. The species with the same level of ploidy had the same karyotype formula: 2m + 6sm + 1st in diploids and 4m + 11sm + 3st in tetraploids, respectively. The amphiploid origin of the tetraploid Lonicera species has been shown. Diploid and tetraploid forms of L. edulis and L. villosa were the particular karyotypes but not the 2x and 4x races of the same species, respectively. Specific differences were revealed in the total chromosome length in the haploid set and in the number of satellites and secondary constrictions. Generic resemblance and specific peculiarities of Lonicera karyotypes indicate a common center of the blue honeysuckle origin and a common initial population of karyotypes which evolved into several phylogenetic branches of the Caeruleae subsection: the Central Asiatic--L. iliensis and L. stenantha; the Siberian--L. altaica, L. edulis, and L. pallasii; the Beringian--L. emphyllocalyx, L. kamtschatica, and L. villosa; the Manchurian--L. boczkarnikowii (L. regeliana), and L. turczaninowii.     

Chromosomes of sixteen European species ofLongitarsus flea-beetles (Coleoptera,Chrysomelidae)

Sixteen species ofLongitarsus have been chromosomally surveyed, showing a continuous range of even numbers from 2n=26 to 2n=32 chromosomes. Among the total of twenty-three known species, about 40% display a 14+Xy male karyotypic formula, the possible modal and most primitive one for the genus. The current taxonomy of species groupings is in good agreement with the chromosome numbers in some cases, but not in others. Also, there is no interrelationship between chromosome numbers and foodplant selection. The number of large bivalents at metaphase I is generally negatively correlated with the diploid value, suggesting the possible role of centric fusions coupled to shifts in the amount of chromatin as the main chromosomal changes in the evolution ofLongitarsus. The karyotypes of a few studied species are composed of metacentric chromosomes, some of them of rather large size, and a minute y-chromosome. A possible example of polymorphism for the chromosome number inL. nigrofasciatus is reported and briefly discussed.     

Cytogenetic investigations on seven Palaearctic weevil species (Coleoptera, Curculionidae).

Cytogenetic investigations were carried out on seven species of bisexual weevils from five subfamilies. The following numbers of chromosomes were found in individual species: 2n = 22, n male = 10 + Xyp in Ptochus porcellus Boh. (Otiorhynchinae); 2n = 40, n male = 19 + Xyp in Larinodontes turbinatus Gyll. (Cleoninae); 2n = 26, n male = 12 + Xyp in Curculio pellitus (Boh.) (Curculioninae); 2n = 30, n male = 14 + Xyp in Acalles camelus (F.), Acalles echinatus (Germ.), Acalles hypocrita Boh. (Cryptorhynchinae), and 2n = 40, n male = 19 + Xyp in Cionus hortulanus (Foucr.) (Mecininae). The heterochromosomes of all the examined species form, in the first meiotic metaphase, a parachute bivalent. The chromosome number and sex determining system of six species and two genera are described for the first time.     

Karyotypic characterization of three weevil species (Coleoptera: Curculionidae, Brachyderini)

Karyotypes of three species, Brachyderes incanus, Brachysomus setiger and Paophilus afflatus, belonging to the tribe Brachyderini, were studied using C-banding technique. The species share the same chromosome number 2n = 22 and meioformula n = 10+Xy(p) at all metaphase 1 plates of spermatid division. Some differences between karyotypes were observed in terms of centromere positions and C-band sizes. Most chromosomes are meta- or submetacentric and form a graded series in respect to length. The chromosomes resemble one another in having a rather small amount of heterochromatin restricted to the pericentromeric region and visible as dark stained blocks mainly during early stages of nuclear division. Only in Brachyderes incanus do larger bands occur at mitotic metaphase and diakinesis. These cytogenetic data are in agreement with karyological findings obtained in other species of Brachyderini so far examined.     

Chromosomes of some Puerto Rican flea beetles (Coleoptera: Chrysomelidae: Alticinae): multiple cytogenetic evolutionary tendencies in the neotropics

Meiotic chromosomes of 10 West Indian flea beetles with restricted distribution, including eight Puerto Rican endemics, were studied. Two species of the cosmopolitan Longitarsus resemble Old World congenerics in having meioformulae 13 + Xy and 14 + Xy, both with one pair of enlarged autosomes. One unidentified species of Aphthona seems to have achiasmatic meiosis. Among the Puerto Rican endemics, repeated fusions have produced low-numbered karyotypes in Homoschema latitarsum (2 + neoXY, now the lowest meioformula known for the Chrysomelidae) and Heikertingerella krugi (4 + neoXY). Three species of Aedmon have 18 + Xy_p as a result of a series of centric fissions from the Polyphagan ancestral condition of nine pairs. Phyllotrupes sp. (a new genus record for Puerto Rico, near P. acutangula ) and Pseudodisonycha portoricensis have 17 + Xy and 16 + Xy, respectively, plus a swarm of supernumerary chromosomes.     

Karyological investigations on seven weevil species (Coleoptera, Curculionidae)

Karyological studies were carried out on seven Palaearctic weevils. The following chromosome numbers were found in individual species, i.e. Otiorhynchus niger (F.), Phyllobius viridearis (Laich.), Phyllobius scutellaris Redt., Phyllobius calcaratus (F.), Polydrusus cervinus (L.), and Brachyderes incanus (L.) 2n = 22, n Male = 10 + Xyp, in Lixus elegantulus (Boh.) 2n = 22, n Male = 21 + Xyp. The heterochromosomes of all the examined species form, in the first meiotic metaphase, a typical parachute bivalent.     

Ag-banded karyotypes in six species of jewel-beetles (Coleoptera, Buprestidae).

The male karyotypes of six species of the buprestid genera Sphenoptera (two species) and Acmaeoderella (four species) were studied after Ag-banding. The following karyotypes were found in individual species: n = 19-23, with predominance of 23, in Sphenoptera scovitzi Fald., 2n = 24 (22 + Xyp) in Sphenoptera mesopotamica Mars., 2n = 18 (16 + Xyr) in Acmaeoderella flavofasciata Pill. & Mitt., Acmaeoderella gibbulosa Men., Acmaeoderella boryi Brulle, Acmaeoderella vetusta Men. In Sph. scovitzi, two chromosome pairs were found to display stable argentophilous blocks. In Sph. mesopotamica, NOR-bearing is one of the large autosomal bivalents. In all species of the genus Acmaeoderella, a nucleolus-like argentophilous body is placed between the X and y chromosomes.     

两种过路黄的核型研究

对国产报春花科珍珠菜属植物过路黄和疏节过路黄的核型进行了首次报道。过路黄染色体数目为２ｎ＝２４,核型公式为２ｎ＝２４＝２ｍ＋４ｓｍ＋６ｓｔ＋１２ｔ,核型类型“３Ａ”;疏节过路黄染色体数目为２ｎ＝２２,核型公式为２ｎ＝２２＝４ｍ＋６ｓｍ＋１０ｔ,核型类型“３Ａ”。     

两种过路黄的核型研究

对国产报春花科（Ｐｒｉｍｕｌａｃｅａｅ）珍珠菜属（Ｌｙｓｉｍａｃｈｉａ）植物过路黄（Ｌ.ｃｈｒｉｓｔｉｎａｅＨａｎｃｅ）和疏节过路黄（Ｌ.ｒｅｍｏｔａＰｅｔｉｔｍ）的核型进行了首次报道。过路黄染色体数目为２ｎ＝２４,核型公式为２ｎ＝２４＝２ｍ＋４ｓｍ＋６ｓｔ＋１２ｔ,核型类型“３Ａ”;疏节过路黄染色体数目为２ｎ＝２２,核型公式为２ｎ＝２２＝４ｍ＋６ｓｍ＋２ｓｔ＋１０ｔ,核型类型“３Ａ”。     

葱属植物物种生物学研究 Ⅰ.葱属6种材料的核型研究

分析了葱属(Allium L.)5个种6个居群的细胞学特征。这些种是太白韭(A.prattii C.H.Wright apud Forb.et Hemsl.),该种包括两个居群(Ⅰ、Ⅱ)。居群Ⅰ:K(2n)=2x=16=9m+1m(SAT)+4sm+2st,居群Ⅱ:K(2n)=2x=16=10m+5sm+1sm(SAT);天蒜(A.paepalanthoides Airy-Shaw):K(2n)=2x=16=14m+2sm+3B;多叶韭(A.Plurifoliatum Rendle):K(2n)=2x=16=14m+2sm+1B;合被韭(A.tubiflorum Rendle):K(2n)=2x=16=12m+2m(SAT)+2sm;峨眉韭(A.omeiense Z.Y.Zhu):K(2n)=2x=22=2m+18sm+2T(SAT)。所研究的6批材料均为二倍体,除合被韭的核型为“1A”型,蛾眉韭的核型为“3A”型外,其余4批材料的核型均为“2A”型。其中峨眉韭和多叶韭的染色体数目为首次报道,天蒜和多叶韭的细胞中首次发现B染色体,并对其相互关系进行了探讨。     

Silver staining of nucleolar organizer regions (NOR) in some species of Hymenoptera (bees and parasitic wasp) and Coleoptera (lady-beetle)

Adaptations of the nucleolar organizer regions (NOR) banding technique using precipitation of silver salts significantly improved the NOR characterization of some species of hymenopterans and one coleopteran. The bee Melipona marginata (2n = 18) showed one metacentric pair of chromosomes with a NOR in the pericentromeric position. The parasitic wasp Mellitobia australica (2n = 12) also showed one metacentric pair with a strongly Ag-positive NOR. The male lady-beetle Cycloneda sanguinea (2n = 18 + Xy(p)) displayed a NOR on a pair of acrocentric autosomes. In the male Euglossa sp. (a haplodiploid species) (n = 21) the NOR were multiple, and occurred in five chromosomes. In the bee Plebeia sp. 1 (2n = 34) the NOR seemed restricted to one of the homologues of a metacentric pair. The systematic advances brought out by using this technique in the context of current theories of karyotypic evolution of these taxa are described and discussed.     

A Chromosomal Study on 7 Species of Smilax L.

The chromosome numbers and karyotypes of 7 species of Smilax L. in Liliaceae (s. 1.) are cytotaxonomically studied in this work. Their karyotypic characters, distinction between the species and the chromosomal basis of sexual differentiation are discussed. The karyotypes of most species are first reported. The results are shown as follows (see Tables 1-4 for the chromosome parameters and the karyotype constitution; Fig. 1 for their idiograms): 1. Smilax nipponica Miq. The species is one of the herbaceous species distributed in East Asia. Two karyotypes, 2n = 26(type A) and 2n = 32 (type B), are found in the species (Plate 1: 1-7). The karyotype of No. 88032 (uncertain of -L--M--S- sexuality) is 2n = 26 = 2m + 6st + 6m + 4sm + 6sm + 2st. The karyotype has 4 pairs of L chromosomes, of which the first three pairs are subterminal, and the 4th is median. The karyotype belongs to 3B. No. 88045 (the male) and No. 88046 (the female) have 2n = 32. Their karyotypes are basically uniform, and both are -L--M-- S 2n=32= 2m+4sm+ 2st+ 2m+4sm+ 6m+ 10sm + 2st, also with 4 pairs of L chromosomes, but the 2nd pair is median, and thus different from the type A. The karyotype belongs to 3B. The first pair of chromosomes of the male are distinctly unequal in length, with the D. V. (0.93) of relative length between them obviously greater than that of the female (0.1). The pair seems to be of sex-chromosomes. Sixteen bivalents (n= 16) were observed at PMCs MI of No. 88045 (Plate 1: 4). The major difference between the karyotypes A and B are greater relative length of L chromosomes in the type A than in the type B, and the increase of chromosome number in the karyotype B mainly due to the increase of st chromosomes. Nakajima (1937)reports 2n= 30 for S. hederacea var. nipponica (=S. nipponica, Wang and Tang, 1980). 2. S. riparia A. DC. This species is also herbaceous, distributed in East Asia. Thirty chromosomes were found in root-tip cells (uncertain of sexuality). The kar -L--M--S-yotype is 2n = 30 = 8st + 6sm + 2st + 6m + 6sm + 2st (Plate 3: 1, 5), consisting mainly of sm and st chromosomes. There are 4 pairs of L chromosomes which are all subterminal and the m chromosomes appear to fall all into S category. Though the karyotype belongs to 3B, it is less symmetrical than that of S. nipponica. The species is karyologically rather different from S. nipponica, therefore. The first pair of chromosomes of this material are unequal in length, and it may be a male. The karyotype of this species is first reported. 3. S. sieboldii Miq. The species is a thorny climbing shrub, distributed in East Asia. At PMCs All, 16 chromosomes (n= 16) were found (Plate 2: 6), in accordance with Nakajima's (1933) report for a Japanese material. 4. S. china L. This species, a thorny climbing shrub, is of a wide distribution range mainly in East Asia and Southeast Asia. Two karyotypes were observed in different populations. (1) The population from Xikou has 2n = 96(6x) = 20st+L- -M- 6t + 6sm + 12st + 52(S) (Plate 3:7), of which the first three pairs of chromosomes are terminal, different from those in the other species. The arm ratios of both L and M chromosomes are larger than 2.0, which resembles those of S. davidiana. (2) PMCs MI of the population from Shangyu shew 15 chromosomes (n 15). The hexaploid of the species is recorded for the first time. Hsu (1967,1971) reported 2n = 30 from Taiwai and Nakajima (1937) recorded n = 30 from Japan, which indicates that the karyotype of the species varies not only in ploidy, but also in number. 5. S. davidiana A. DC. The somatic cells were found to have 32 chromosomes, and PMCs MI shew 16 bivalents (Plate 2: 1-5). The karyotype is 2n = 32=-L- -M- -S 8st + 4sm + 4st + 8sm + 8st. The karyotype belongs to 3B, and is less symmetrical than those in herbaceous species. The D. V. (0.20) of relative length between the two homologues of the first pair is slightly larger in the male than in the female (0.14), and it is thus difficult to determine whether they are sexual chromosomes or not. 6. S. glabra Roxb. The species is a non-thorny climbing shrub, distributed in East Asia and Southeast Asia. 32 chromosomes were found in somatic cells. The -L- -M- - Skaryotype is 2n= 32= 8st + 10st+6sm+8st (Plate 3: 2, 6),with only 3 pairs of sm chromosomes (12, 13 and 16th). The karyotype is more asymmetric than that of S. davidiana, although it is also of 3B (Table 1). The karyotype is first reported for the species. 7. S. nervo-marginata Hay. var. liukiuensis (Hay.) Wang et Tang The variety has a relatively narrow distribution range, mainly occurring in eastern China. The chromosomal number of somatic cells is 2n= 32 (Plate 3: 3-4). The karyotype is -L- -M- -S 2n = 32 = 2sm + 6st + 2sm + 2st + 2m + 6sm + 12st, evidently different from that of S. glabra. The first pair of chromosomes are submedian, and much longer than the 2nd to 4th pairs. The ratio in length of the largest chromosome to the smallest one is 4.3. The symmetric degree is of 3C, a unique type. The karyotype of the species is reported for the first time. In Smilax, the known basic numbers are 13, 15, 16 and 17. The two herbaceous species distributed in East Asia have three basic numbers: 13, 15 and 16, while the woody species studied mainly have 16, with no 13 recorded. Mangaly (1968) studied 8 herbaceous species in North America and reported 2n=26 for them except S. pseudo-china with 2n=30. Mangaly considered that a probably ancestral home of Smilax, both the herbaceous and woody, is in Southeast Asia and the eastern Himalayas, and speculated that the ancestral type of Sect. Coprosmanthus is possibly an Asian species, S. riparia. The karyotypes of the two herbaceous species in East Asia consist mostly of sm and m chromosomes, whereas those for the North American species are all of st chromosomes. Based on the general rule of karyotypic evolution, i.e. from symmetry to asymmetry, his speculation seems reasonable. Researches on sex-chromosomes of Smilax have been carried out since 1930 (Lindsay, 1930; Jensen, 1937; Nakajima, 1937; Mangaly, 1968), and they are generally considered to be the largest pair, but there is still no adequate evidence. The result of our observation on S. nipponica may confirm that the first pair of chromosomes of this species is XY type of sex-chromosomes. Chromosomes of the genus are small and medium-sized, varying between 1-6 μm, slightly larger in herbaceous species than in woody ones, larger in the karyotype of 2n=26 than in that of 2n=32. Based on karyotype constitution of the above 5 species, the karyotype in the genus is characterized by 4 pairs of L chromosomes and 2-5 pairs of M chromosomes, and mostly st and sm chromosomes, and by rather asymmetrical 3B type. The degree of symmetry in the above 5 species is from Sect. Coprosmanthus to Sect. Coilanthus, and herbaceous species towoody ones.     

五种苏铁属植物的核形态

报道了苏铁属（Cycas L.)5种植物的染色体数目和核型,除多歧苏铁外,其他种均为首次报道。5个种的体细胞中期染色体核型公式分别为：滇南苏铁C.diannanensis K(2n)=2x=22=2m 4sm 4st 12T;潭清苏铁C.tanqingii K(2n)=2x=22=2m 8sm 2st 10T;多歧苏的Cmultipinnata K(2n)=2x=22=4m 8st 2st 8T;巴兰萨苏铁C.balansae K(2n)=2x=xx=2m 4sm 6st 10T。石山苏铁C.miquelii K(2n)=22=2m 6sm(1SAT) 4st 10T;核型均属于3B型。本研究结果支持苏铁属植物的核型从不对称进化的观点;同时,支持将巴兰萨苏铁和石山苏铁归入攀枝花苏铁组的台湾苏铁亚组的观点。     

Cytogenetics, cytotaxonomy and chromosomal evolution of Chrysomelinae revisited (Coleoptera, Chrysomelidae)

Nearly 260 taxa and chromosomal races of subfamily Chrysomelinae have been chromosomally analyzed showing a wide range of diploid numbers from 2n = 12 to 2n = 50, and four types of male sex-chromosome systems. with the parachute-like ones Xy(p) and XY(p) clearly prevailing (79.0%), but with the XO well represented too (19.75%). The modal haploid number for chrysomelines is n = 12 (34.2%) although it is not probably the presumed most plesiomorph for the whole subfamily, because in tribe Timarchini the modal number is n = 10 (53.6%) and in subtribe Chrysomelina n = 17 (65.7%). Some well sampled genera, such as Timarcha, Chrysolina and Cyrtonus, are variable in diploid numbers, whereas others, like Chrysomela, Paropsisterna, Oreina and Leptinotarsa, are conservative and these differences are discussed. The main shifts in the chromosomal evolution of Chrysomelinae seems to be centric fissions and pericentric inversions but other changes as centric fusions are also clearly demonstrated. The biarmed chromosome shape is the prevalent condition, as found in most Coleoptera, although a fair number of species hold a few uniarmed chromosomes at least. A significant negative correlation between the haploid numbers and the asymmetry in size of karyotypes (r = -0.74) has been found from a large sample of 63 checked species of ten different genera. Therefore, the increases in haploid number are generally associated with a higher karyotype symmetry.     

Karyotype evolution in Curimatidae (Teleostei, Characiformes) from the Amazon region. II. Centric fissions in the genus Potamorhina

Using cytogenetic analysis following Giemsa staining, nucleolar organizer region (NOR) staining, and C-banding, three distinct karyotypes in three species of curimatids belonging to the fish genus Potamorhina were identified: 2n = 54/44 M + 10 SM (P. pristigaster), 2n = 56/52 M + 2 SM + 2 ST (P. latior), and 2n = 102/2 M + 2 SM + 98 A (P. altamazonica). A 2n = 54 was considered to be the ancestral diploid number and the different karyotypes were probably the result of centric fissions. Both the NOR pattern and constitutive heterochromatin pattern are species specific.     

Karyotype Analysis of Hippophae L. in China

The present paper reports karyotypes in 4 species and 5 subspecies of Hippophae L. in China. It is found that all of the species and subspecies are diploid, with 2n=24, their complements are made of m and sm chromosomes and of them only H. thibetana has a pair of satellites. All of these karyotypes are symmetrical and primitive. It can be simplied as follows: Hippophae neurocarpa 2n = 2x = 24 = 18m+6sm, H. thibetana 2n = 2x = 24 = 14m (2sat)+ 8sm, H. rhamnoides L. ssp. gyantsensis 2n=2x=24= 18m+ 6sm, H. salicifolia 2n = 2x = 24 = 10m + 14sm, H. rhamnoides L. ssp. sinensis 2n=2x= 24 = 18m + 6sm, H. rhamnoides L. ssp. turkestanica 2n = 2x = 24 = 20m + 4sm; Hi. rhamnoides L. ssp. yunnanensis 2n = 2x = 24 = 14 + 10sm, H. rhamnoides L. ssp. mongolica 2n = 2x = 24 = 16m + 8sm.     

香港喜盐草属植物的核型分析

报道了分布于我国香港特别行政区的喜盐草属(Halophila Thou.) 2种植物的染色体数目和核型,其体细胞中期染色体数目均为2n=18。它们的核型公式分别为：喜盐草(H. ovalis (R. Br.) Hook. f.) 2n=2x=18=4m+14sm;贝克喜盐草(H. beccarii Asch) 2n=2x=18=8m+10sm(首次报道)。核型均属于2B型。     

5种珍珠菜属植物的核型分析

对 5种珍珠菜属 (LysimachiaL .)植物的核型进行了研究 ,其中巴东过路黄 2n =2x =2 4 =6m 4sm 6st 8t、光叶巴东过路黄 2n =2x =2 4 =6m 4sm 6st 8t、临时救 2n =2x =2 4=2m 4sm 4st 14t的核型属首次报道。过路黄 2n =2x =2 4 =2m 4sm 6st 12t和星宿菜2n =2x =2 4 =2 0m 4sm与前人报道的一致。本文还对该属已报道的 17种植物的核型资料进行了总结和比较分析 ,对珍珠菜属植物的核型进化方向作了初步推测。另外对一存疑过路黄(2n =2x =2 4 =2m 6sm 4st 12t)与其近缘种的核型进行了比较研究。