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1.
Experimental indoxacarb powder and gel baits were evaluated in the laboratory, and a gel bait was evaluated in subsequent field studies against the German cockroach, Blattella germanica (L.). In continuous exposure tests, LT50 values were 1.90 and 1.10 d for 0.25 and 1% indoxacarb powder baits, respectively. However, 0.25% indoxacarb gel bait had an LT50 value of 0.68 d, similar to a 0.05% abamectin gel bait formulated with the same bait base. There was no difference in toxicity between fresh and 7-d-old gel bait deposits. A pyrethroid-resistant strain of German cockroaches was significantly resistant to both abamectin and indoxacarb gel baits. Gel bait contained approximately 40% water, desiccated rapidly at 25-28 degrees C and 30-45% RH, but did not rehydrate when held at 56.7% RH for 3 d. Powder indoxacarb baits contained <1% water and did not desiccate or gain water. Indoxacarb gel bait (0.25%) was relatively nonrepellent (approximately 30%) and had positive maximum performance index values (approximately 100) in Ebeling choice box experiments. In field experiments in cockroach-infested kitchens, the 0.25% indoxacarb gel bait significantly reduced visual counts of German cockroaches approximately 74% at 3 d and >95% at 14 d. Indoxacarb baits are toxic, relatively nonrepellent, and can significantly reduce German cockroach populations.  相似文献   

2.
An experimental 2.15% imidacloprid gel bait containing approximately 44% water was evaluated in laboratory and field studies against the German cockroach, Blattella germanica (L). In continuous exposure tests, toxicity and presumably bait consumption varied with cockroach stage, deprivation of competitive food, and temperature. The LT50 values for cockroaches provided with competitive food ranged from approximately 0.9 h for adult females to 190 h for small nymphs. The LT50s for cockroaches not provided competitive food ranged from approximately 1.7 h for adult females to approximately 31 h for adult males. The LT50s decreased exponentially with temperature between 10 and 30 degrees C. Even though the bait was significantly more repellent (approximately 38%) than an untreated control (approximately 14%) when tested in Ebeling choice boxes, performance index values were positive and increased to nearly 100 (indicating high mortality and low repellency) after 14 d. When applied at 15-45 g per kitchen, the bait significantly reduced German cockroach trap catch in infested homes during a 4-wk period. There was a approximately 50% reduction after 1 wk and approximately 80% reduction 4 wk after treatment.  相似文献   

3.
The susceptibility of American cockroaches, Periplaneta americana (L.); smoky brown cockroaches, P. fuliginosa (Serville); oriental cockroaches, Blatta orientalis L.; German cockroaches, Blattella germanica (L.); and brownbanded cockroaches, Supella longipalpa (F.), to Steinernema carpocapsae Weiser (All strain) was evaluated under laboratory conditions. A 1-ml water suspension containing 500,000 nematodes was placed on filter paper in a petri dish or the pad of a bait station. German, brown-banded, oriental, and smoky-brown cockroaches died within 1 d after placement in the petri dishes. The relative order for the LT50s were American greater than oriental greater than smoky-brown greater than brown-banded = German. All cockroaches actively groomed nematodes from legs and antennae of forced (petri dish) exposure. The LT50s for S. carpocapsae for nonforced (bait station) exposure were significantly greater than those for forced exposure. The LT50s were 3.25, 4.13, 9.86, and 11.38 d for brown-banded, German, oriental, and smoky-brown cockroaches, respectively. The relative order of the LT50s after forced (American greater than oriental greater than smoky-brown greater than German = brown-banded) and nonforced (American greater than smoky-brown greater than oriental greater than German greater than brown-banded) exposure to S. carpocapsae was inversely related to the moisture of their preferred habitats.  相似文献   

4.
Ten field-collected strains of the German cockroach, Blattella germanica (L.), with varying levels of pyrethroid resistance were tested for possible cross-resistance to abamectin administered as a bait. The time-mortality method was used for comparison of response with a known susceptible strain in 10-d feeding experiments. Essentially no resistance to abamectin was found. Extensive feeding inhibition occurred after a few days, but the amount of abamectin consumed varied substantially from strain to strain. Abamectin may have considerable potential as a cockroach bait insecticide.  相似文献   

5.
The acceptability of dietary allopurinol to German cockroaches, Blattella germanica (L.), was assessed. In diet choice tests between rat chow with or without 0.1% w/w allopurinol there were no significant differences in nymphal development, feeding duration, visits or consumption. The LT50 of cockroaches fed a choice of diets was 3 weeks greater (7.88) than those fed non-choice a 0.1% allopurinol diet. Female cockroaches provided a choice of diets aborted a significantly greater percentage of their oothecae (98.6%) than those fed the untreated diet (1.7%). Choice arena tests of 2% allopurinol in rat chow significantly reduced cockroach populations compared with untreated controls. After 6 weeks, populations were reduced by approximately 50%, and 97% after 14 weeks. These results indicate that allopurinol is acceptable to B. germanica as a dietary supplement which could be used in baits for cockroach control.  相似文献   

6.
Gel and paste bait formulations containing boric acid or hydramethylnon were evaluated in field and laboratory experiments with the German cockroach, Blattella germanica (L.). Bait formulations contained 14-62% water by weight and lost the most water after 1 d of desiccation. Bait age and water content did not alter the toxicity (LT50) of boric acid-based baits. Toxicity of hydramethylnon (Maxforce) bait, however, increased with bait age. In continuous exposure tests without harborage, boric acid formulations had LT50s of 8.5 and 10.1 d for Blue Diamond and IT WORKS, respectively. Maxforce bait had an LT50 of 4.2 d. LT50s determined with cardboard harborage or in Ebeling choice boxes were 1.5 to 2.2 times lower than those from continuous exposure tests without harborage. Relative repellency, measured as the mean percent of live cockroaches in the light side of the choice box, was greatest for IT WORKS (21.4%) and Maxforce (25.4%) formulations, followed by Blue Diamond (10.9%). A performance index that combined choice box repellency and mortality data indicated that Maxforce had the greatest potential for field effectiveness. Blue Diamond and Maxforce significantly reduced German cockroach populations in the field. Maxforce performed best in field trials when applied at 10 or 20 placements per apartment. Apartments treated with IT WORKS had significantly greater cockroach trap catches. Factors that may affect the predictive power of the performance index are discussed.  相似文献   

7.
Bait formulations containing boric acid, chlorpyrifos, or hydramethylnon were evaluated in field and laboratory experiments against the German cockroach, Blattella germanica (L.). In continuous exposure tests, chlorpyrifos formulations produced 100% mortality in less than 24 h, boric acid formulations had LT50's of approximately 5.5 d, and hydramethylnon had an LT50 of 0.86 d. LT50's estimated in Ebeling choice boxes were 1.6 to 10.8 times greater than those from continuous exposure tests for chlorpyrifos formulations and 2.5 times greater for the hydramethylnon formulation. The same LT50's were produced by choice box and continuous exposure tests for boric acid formulations. Relative repellency, measured as the mean percentage of live cockroaches in the light side of the choice box, was greatest for chlorpyrifos formulations, followed by boric acid formulations, then hydramethylnon. A performance index (PI) that combined choice box repellency and mortality data indicated that hydramethylnon (Combat) had the greatest potential for field effectiveness. Only hydramethylnon significantly reduced German cockroach populations in the field. Apartments treated with the IT WORKS formulation of boric acid bait had significantly greater cockroach trap catches. The PI is an accurate predictor of the field performance of toxic baits for German cockroach control.  相似文献   

8.
Several insecticide bait formulations were evaluated for their attractiveness to cockroaches in olfactometer assays in the laboratory and in trapping experiments in the field. Included in the assays were bait stations, gels, pastes, and a powder that contained one of the following active ingredients: abamectin, boric acid, chlorpyrifos, or hydramethylnon. There were significant differences among the baits in their attractiveness to the German cockroach, Blattella germanica (L.). In trapping experiments, Avert powder (abamectin), Maxforce station and gel, and Siege gel (all hydramethylnon) were consistently attractive to B. germanica adults and nymphs. Laboratory olfactometer assays with adult males confirmed these results and showed that nymphs were as responsive as males whereas females were less responsive. Our bioassays also demonstrate that attractiveness of bait can be dramatically affected by the age of the bait. One week of aging significantly reduced the attractiveness of Avert powder in both laboratory and field assays. Aging, however did not diminish the attractiveness of Maxforce gel, indicating that the formulation may be critical for retention of attractiveness of baits. Baits that were most attractive to the German cockroach were also the most attractive to nymphs and adults of the brownbanded cockroach, Supella longipalpa (F.).  相似文献   

9.
The insecticide resistance status of the German cockroach, Blattella germanica (L.), against permethrin was evaluated with three different bioassay methods. The LT50 and KT50 in adults were measured by glass jar test, the LT50 and KT50 in nymphs were assessed by glass petri dish-test, and the LD50 in adults and nymphs was measured by topical application. The results indicate that in all three bioassays, cockroaches showed different degrees of resistance to permethrin. Resistance ratios of 6.7-, 12.8-, and 7.5-fold for KT50, LD50, and LT50, respectively, were obtained for adult cockroaches compared with those from a susceptible strain. Similarly, resistance ratio values of 17-fold for KT50, 8-fold for LD50, and 6.4-fold for LT50 were obtained for nymphs compared with those from a susceptible strain. Although both adults and nymphs exhibited a similar level of susceptibility to permethrin, adults showed a higher degree of susceptibility to topical application than to the other exposure method. The order of sensitivity (based on resistance ratio values) was topical application (LD50) > LT50 > KT50 in adults. For nymphs, the resistance ratio in the knockdown test was higher than in other tests (resistance ratio = 17-fold). Therefore, the order of test sensitivity based on comparisons of resistance ratio values was KT50 > topical application (LD50) > LT50 in nymphs. Topical application exhibited a higher sensitivity than the LT and KT methods in adults. Thus, the results suggest that topical application and knockdown testing methods are the most sensitive assays for measuring resistance of adult and nymphal German cockroaches, respectively, to permethrin.  相似文献   

10.
Consumption was measured of three commercially available cockroach gel baits (0.01 and 0.05% fipronil and 0.6% indoxacarb) by two pest blattellid (German cockroach, Blattella germanica L., and brownbanded cockroach, Supella longipalpa Fabricius) and three pest blattid [oriental cockroach, Blatta orientalis L., American cockroach, Periplaneta americana L., and smokybrown cockroach, Periplaneta fuliginosa (Serville)] species (Dictyoptera), and direct and secondary effects were quantified. All three baits were greatly preferred for consumption over dog food; however, virtually all consumption (ca. 98%) by pest blattids was gel baits containing sugar feeding stimulants and water. Pest blattid greater preference for gel baits was probably due to their greater need for nutrients in baits due to their greater cuticular water permeability and higher metabolism than the pest blattellids. Brownbanded cockroaches had lowest percentage gel bait selection. Pest blattellids consumed greater amounts of bait per g body weight than pest blattids. Cockroaches consumed more active ingredient than needed to cause mortality; however, based on bait consumption, a 30‐g tube of gel bait potentially killed from 394 to 6 966 adult cockroaches, depending on species. Mortality for all cockroach species was faster for adults (≥3 days) than for nymphs (≥7 days); however, most brownbanded cockroaches exposed to indoxacarb survived despite consuming 1.5‐ to >3‐fold more than other baits, suggesting low enzyme production by brownbanded cockroaches and consequently lower conversion of indoxacarb into its toxic form. Besides direct mortality, German cockroaches died from indirect effects: exposure to debris from other cockroaches that had direct access to the gel baits or bait contact without ingestion. Although maximization of bait consumption is important, factors that enhance secondary mortality and contact toxicity should also be considered.  相似文献   

11.
A Beauveria bassiana bait formulation was evaluated for its efficacy in the laboratory and field against German cockroaches, Blattella germanica (L.), and American cockroaches, Periplaneta americana (L.). The LT50 values were 46 and 119?h, respectively, for B. germanica and P. americana. The cockroach species were lured and killed effectively when linear distance was about 5?m between B. bassiana bait and fresh mouse food. In the field, more than 90% relative decline in density was found 14?d after treatment, which lasted for 90?d. In conclusion, laboratory and field studies demonstrated the high efficacy of a B. bassiana bait formulation against common cockroach species.  相似文献   

12.
An aqueous extract of German cockroach, Blattella germanica (L.), fecal material was evaluated for inducing trail-following behavior in German cockroaches. In arena tests the fecal extract was found to stimulate trail following in 74% of adult male cockroaches. Significantly fewer cockroaches (22%) followed water-treated (control) trails. Residual activity of the fecal extract trails was evaluated by bioassay after the trails had been stored in the refrigerator or in the open air. Although trails stored in the refrigerator showed no decline in activity after 14 d, those stored in the open air declined significantly after 3 d, inducing only 40% of adult male cockroaches to follow the trail. After 7 d the activity of trails stored in the open air was further reduced to 23%. The ability of fecal-extract trails to influence trap catch in monitoring stations was determined by bioassay. Paper trails treated with fecal extract or water were positioned between cockroach harborages and monitoring stations inside 122-cm2 arenas. The presence of the fecal extract-treated trails significantly enhanced trap catch. Mean catch in the traps with fecal extract trails was 28 cockroaches compared with a mean of 11 cockroaches in the control traps. The trap catch ratios of adults to nymphs in the treated and control treatments were not significantly different.  相似文献   

13.
A gel bait-resistant German cockroach, Blattella germanica (L.), strain Cincy was collected in Cincinnati, OH. This strain exhibited a high level of behavioral resistance to Avert (0.05% abamectin) and Maxforce FC (0.01% fipronil) gel baits. Topical application assays indicated moderate levels of physiological resistance of the Cincy strain to abamectin and fipronil. Resistance ratios (based on LD50 values from topical applications) to abamectin and fipronil were 2.5 and 8.7, respectively. The Cincy strain of had a significantly lower LD50 value to abamectin than a nonaverse field strain (Dorie) and similar LD50 values to fipronil as the Dorie strain. The aversion behavior (avoidance of gel baits) was therefore caused by food ingredients in the gel baits. The Cincy strain showed avoidance of agar containing fructose, glucose, maltose, and sucrose, which are phagostimulants to the laboratory strain. Modifications of the inert ingredients in the Maxforce FC gel bait significantly improved the efficacy against the Cincy strain. The Cincy strain produced significantly smaller oothecae and lower numbers of eggs in each egg capsule than the nonaverse Jwax and Dorie strains of cockroaches, suggesting fitness costs are associated with resistance.  相似文献   

14.
Female German cockroaches, Blattella germanica (L.) (Dictyoptera: Blattellidae) and nymphs (stages 1, 3 and 6) were used to test the attractiveness and the feeding stimulation of several commercial gel formulations of insecticide food baits in laboratory binary choice tests. The four toxic gel baits tested were: 'Avert' (0.05% abamectin=avermectin B1), 'Drax' (33.3% boric acid), 'Goliath' (0.05% fipronil) and 'Maxforce' (2.15% hydramethylnon). The attractiveness of a bait was estimated by the number of cockroaches that chose it first, and by their tendency to change to it secondarily. Feeding stimulation was estimated by the mean duration of feeding bouts and total duration of food intake. Variability of these factors was assessed in relation to age of cockroaches and to age of baits. With a few exceptions, cockroach age did not affect performance in any of these tests. Attractiveness to B. germanica ranked 'Goliath' gel higher than 'Avert', 'Drax' and 'Maxforce' gels, whether or not the active ingredient fipronil was present in 'Goliath' gel. Feeding stimulation ranked 'Avert' and 'Maxforce' gels higher than 'Goliath' gel, and 'Drax' gel was inferior. With ageing up to 3 months, the feeding stimulation power of 'Goliath' and 'Maxforce' gels increased, whereas that of 'Avert' fluctuated. Assuming that all types of bait effectively kill cockroaches once ingested, these results illustrate the importance of behavioural observations on bait efficiency and show that ranking of these different food baits varies in relation to the parameter analysed.  相似文献   

15.
Topical and oral toxicity of sulfluramid (N-ethyl perfluorooctane sulfonamide) were determined for the German cockroach, Blattella germanica (L.). The topical LD50 of sulfluramid was 175.6 micrograms/g for adult males, 117.8 micrograms/g for adult females, and 122.3 micrograms/g for gravid adult females. Ingestion increased toxicity approximately 1.4 times for adult male B. germanica. Twenty-four hours after topical treatment with 20 micrograms/insect sulfluramid, the percentage of female cockroaches that dropped their oothecae increased approximately 50% compared with controls treated with acetone. Sulfluramid also decreased oothecal hatch of both dropped and retained oothecae. Approximately 90% of oothecae from untreated females hatches, whereas less than 20% hatched from females treated with 20 micrograms/insect. Mean time for oothecal hatch increased linearly with increasing sulfluramid concentration. In arena studies in Ebeling choice boxes, LT50's ranged between approximately 2.3 and 3.9 d for a 0.331 mg/cm2 deposit and a 1.5% bait, respectively. Higher concentrations of sulfluramid were more repellent in both bait and residual formulations. Performance index values indicated excellent potential field efficacy. Field trials with 1.0 and 1.5% (AI) baits showed up to a 71.3% reduction in cockroach numbers. Baits controlled cockroaches throughout the 12-wk test.  相似文献   

16.
Horizontal transmission of insecticide occurs when foragers contact or ingest an insecticide, return to the aggregation or nest, and translocate the insecticide to the shelter and its vicinity. Relatively more sedentary members of the population then contact or eat the translocated insecticide and die. We evaluated three different methods of delivering fipronil to adult male German cockroaches, Blattella germanica (L.), for their potential to cause such secondary mortality in various developmental stages of the cockroach. Adult males topically treated with 5 ng of fipronil (approximately LD99) caused low mortality in untreated nymphs and no mortality in untreated adults within the same aggregation. Males exposed to residual fipronil on a glass surface translocated more insecticide, resulting in higher mortality of cockroaches they contacted, but only early instars were affected and no adult mortality was observed. Ingested fipronil bait, however, was most effectively translocated, and caused high mortality of untreated adults and nymphs. Ingestion of fipronil also caused greater secondary kill compared with a topical application of 25 ng, approximately the same amount recovered from the exterior of males that ingested 1 mg of 0.05% fipronil bait. Secondary mortality in the untreated population was significantly affected by the duration of contact between the treated and untreated cockroaches, the quantity and freshness of excretions from the treated insects, and the accessibility of the secretions to untreated cockroaches. The mechanisms that cause secondary kill may include ingestion of excreted fipronil residues, cannibalism of bait-fed cockroaches, as well as contact with fipronil-contaminated substrates.  相似文献   

17.
Toxicity of fipronil to German and American cockroaches   总被引:2,自引:0,他引:2  
Topical and oral toxicity of fipronil, compared to chlorpyrifos, was determined for the German cockroach, Blattella germanica (L.), and American cockroach, Periplaneta americana (L.). Fipronil and Combat bait matrices were evaluated for their attractancies to both species. In the topical toxicity tests, LD50's of fipronil, at 72 h after topical application, were 0.03 and 0.02 µg/g for adult B. germanica and P. americana, respectively. Fipronil was significantly more toxic than topically applied chlorpyrifos (LD50's were 0.06 and 0.16 µg/g for B. germanica and P. americana, respectively). The oral toxicity of fipronil and chlorpyrifos in Petri dish experiments, against both species, was affected by stage (for B. germanica), diet concentration, and feeding assay. Fipronil caused higher mortality of B. germanica than chlorpyrifos in two feeding assays (continuous and abbreviated). Both compounds were equally toxic to adult males of P. americana at all rates. Fipronil caused higher nymphal mortality than chlorpyrifos 48–72 h after exposure in both feeding assays. In large population chamber tests, fipronil bait was more effective and faster in killing P. americana than Raid and Combat. LT50's were 0.8, 2.4, and 7.6 d for fipronil, Raid (a.i. = chlorpyrifos), and Combat (a.i. = hydramethylnon) baits, respectively. Mortality reached 96.5, 93.4, and 84.6%, respectively, at the end of the 14 d test. In the bait attractancy tests, both strains of B. germanica were attracted similarly to fipronil and Combat bait matrices. P. americana were attracted more to fipronil than to Combat bait matrix or to other alternative foods.  相似文献   

18.
五种蜚蠊的生物学特性和综合治理   总被引:5,自引:3,他引:5  
孙耘芹  李梅  何凤琴  齐欣 《昆虫知识》2004,41(3):216-222
介绍了分布广泛和危害严重的室内 5种常见蜚蠊 ,德国小蠊、美洲大蠊、黑胸大蠊、澳洲大蠊和褐斑大蠊的生物学特性和行为习性以及德国小蠊为何能成为室内优势种的原因。蜚蠊的综合治理系统包括 :检查和调查、环境治理、非化学防治、杀虫剂防治、昆虫信息化合物和昆虫生长调节剂的防治和其它防治方法。对残留和非残留杀虫剂和剂型的基本概念和应用做了解释 ,并分别介绍了对每种蜚蠊的具体治理措施。  相似文献   

19.
The effects of contamination of insecticidal bait formulations, by using mint oil and silica aerogel, were evaluated in a series of laboratory experiments against the German cockroach, Blattella germanica (L.). Bait consumption at 3 d for uncontaminated baits ranged from 0.01 g for Avert dust to 0.399 g for Combat dry bait with hydramethylnon presented in a station. LT50 values for uncontaminated gel bait deposits ranged from 0.4 d for PreEmpt containing imidacloprid to 4.1 d for Maxforce containing hydramethylnon. As a group, significantly more gel bait was consumed than solid formulations even when both formulations had similar concentrations of the same active ingredient. As a result, gel baits were significantly more toxic than solid formulations. Application of mint oil directly to bait deposits significantly decreased bait consumption and increased overall LT50 values. When bait formulation types were examined individually, there was no difference in consumption or toxicity between contaminated and uncontaminated gel formulations. Contaminated solid baits, however, had significantly greater LT50 values and less consumption than uncontaminated solid baits. Gel formulations probably absorbed the contaminants and removed them from the surface of the bait deposits resulting in normal bait consumption and toxicity. Gel and solid bait deposits, inside plastic exposure stations or alone with no station and placed onto mint oil-contaminated substrates, had significantly lower bait consumption and greater LT50 values than baits placed on uncontaminated substrates. Contamination of a baited area is more likely than contamination of just the bait deposit and therefore a more realistic test of the effects of contamination on bait performance. The importance of contamination to the performance of cockroach baits is discussed.  相似文献   

20.
The toxicity of fatty acid salts to German, Blattella germanica (L.), and American cockroaches, Periplaneta americana (L.), was evaluated. Potassium and sodium laurate caused up to 95% mortality of German cockroaches and 100% mortality of American cockroaches. Even-numbered potassium fatty acid salts, C8-C18 were assessed for toxicity at 0.125, 0.25, 0.5, 1, and 2% concentrations by a 30-s immersion of cockroaches. The more soluble of the fatty acid salts at 2% concentration caused 65-95% mortality of German cockroaches and 100% mortality of American cockroaches. Potassium oleate, C18, was most toxic to both German (LC50 = 0.36%) and American (LC50 = 0.17%) cockroaches. Fatty acid salt solutions on a substrate were tested by placing cockroaches in contact with treated floor tiles immediately after application (wet) or after the solutions had dried. Sodium laurate and potassium caprate caused mortality of German (62 +/- 17.4 and 58 +/- 12.6%, respectively) and American cockroaches (52 +/- 18.5 and 28 +/- 4.9%, respectively) on wet tiles, whereas potassium oleate caused mortality of German cockroaches (67 +/- 14.1%) only. Dry fatty acids caused no mortality among exposed cockroaches. Fatty acid salt solutions can be effective in killing German and American cockroaches but only when insects are thoroughly wetted with 1-2% fatty acid salt solutions.  相似文献   

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