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1.
Previous reports indicate that applications of imidacloprid, a neonicotinoid insecticide, can lead to population buildups of twospotted spider mite, Tetranychus urticae Koch, in the field. Moreover, laboratory studies showed enhanced fecundity of T. urticae after an imidacloprid treatment. In this study, experiments were conducted in the greenhouse to investigate the potential effects of imidacloprid and several other neonicotinoid insecticides on fecundity, egg viability, preimaginal survivorship, and sex ratio of T. urticae (German strain WI) on French beans, Phaseolus vulgaris L. Four insecticides, i.e., imidacloprid (Confidor 200SL), thiacloprid (Calypso 480 SC), acetamiprid (Mospilan 70 WP), and thiamethoxam (Actara 25 WG), were tested at field-relevant (100, 120, 125, and 95 ppm) and sublethal doses (10, 12, 12.5, and 9.5 ppm), respectively. Both spray and drench applications were tested. At field-relevant doses, fecundity of T. urticae decreased and was lower in the treatments compared with the untreated control, whereas preimaginal survivorship and proportion of female offspring (i.e., sex ratio) were lower compared with the control. At sublethal doses, no significant differences were found among the treatments. Data on egg viability, preimaginal survivorship, and sex ratio at sublethal doses followed the same trends as at field-relevant doses. In an additional experiment, the metabolism of imidacloprid into monohydroxy-imidacloprid, olefine, guanidine, and 6-chloronicotinic acid was compared with the oviposition pattern of T. urticae. These findings are discussed with regard to previous laboratory and field observations of imidacloprid-induced fertility increases in T. urticae.  相似文献   

2.
Tetranychus urticae Koch (Acari: Tetranychidae) is an important pest of clementine mandarins, Citrus reticulata Blanco, in Spain. As a first step toward the development of an integrated crop management program for clementines, dispersion patterns of T. urticae females were determined for different types of leaves and fruit. The study was carried out between 2001 and 2003 in different commercial clementine orchards in the provinces of Castelló and Tarragona (northeastern Spain). We found that symptomatic leaves (those exhibiting typical chlorotic spots) harbored 57.1% of the total mite counts. Furthermore, these leaves were representative of mite dynamics on other leaf types. Therefore, symptomatic leaves were selected as a sampling unit. Dispersion patterns generated by Taylor's power law demonstrated the occurrence of aggregated patterns of spatial distribution (b > 1.21) on both leaves and fruit. Based on these results, the incidence (proportion of infested samples) and mean density relationship were developed. We found that optimal binomial sample sizes for estimating low populations of T. urticae on leaves (up to 0.2 female per leaf) were very large. Therefore, enumerative sampling would be more reliable within this range of T. urticae densities. However, binomial sampling was the only valid method for estimating mite density on fruit.  相似文献   

3.
Fumigant activity of 34 commercial essential oils was assessed on female adults and eggs of twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) at three temperatures (5, 15, and 25 degrees C). Common thyme, cinnamon, and lemongrass oils were equally effective on twospotted spider mite adults showing 85.8-100% mortality at 5 and 10 microl/liter air at 25 degrees C. At a lower temperature of 15 degrees C, lemongrass and peppermint resulted in > or =90% mortality of adults at 10 microl/liter air. Only lemongrass was relatively active at 5 microl/liter air, at 15 degrees C. At 5 degrees C, lemongrass and peppermint caused significantly higher adult mortality than controls but only at 10 microl/liter air. Common thyme oil showed the highest ovicidal activity at 5 microl/liter air at 25 degrees C. Among the main components of common thyme and lemongrass oils, citral was lethal to twospotted spider mite adults at all tested temperatures. Carvacrol, thymol, and citral caused the same inhibitory effects on the hatch of twospotted spider mite eggs at 25 degrees C. However, citral was more active than other compounds to twospotted spider mite eggs at 15 degrees C. Therefore, we conclude that citral has the best potential for development as a fumigant against twospotted spider mite on agricultural products harvested late in the growing season.  相似文献   

4.
Experiments were conducted to assess the damage of the glasshouse cucumber by twospotted spider mite, Tetranychus urticae Koch, and to investigate when the economic yield begins to decrease after T. urticae infestations. To assess the damage, dry matter partitioning in the cucumber plant was quantified and plant growth analyses were conducted at four different T. urticae infestation levels. T. urticae infestations decreased leaf productivity by reducing the total number of leaves per plant. Approximately 14% reductions of total leaf areas could result in significant yield loss. The decreased leaf productivity by T. urticae feeding caused biomass reductions and altered the pattern of dry matter partitioning in the plant; damaged plants accumulated more dry matter in the leaf, and partitioning of dry matter to fruits was hindered. The economic yield of cucumber began to significantly decrease as early as 4 wk after heavy mite infestations. This study also showed the seasonal differences in T. urticae-cucumber damage interactions among mite infestation levels.  相似文献   

5.
Lycopersicon hirsutum Dunal is very resistant to arthropod herbivory, and research on causes of resistance has often implicated trichomes and their secretions. To better understand relationships among resistance, repellency, and 2,3-dihydrofarnesoic acid, a trichome-borne sesquiterpenoid spider mite repellent, two tomato, Lycopersicon esculentum Miller, varieties were interbred with a highly resistant, spider mite repellent accession (LA1363) of L. hirsutum. Backcross and F2 generations were produced with each tomato variety. Whole leaves of 99 hybrids were bioassayed with twospotted spider mites, Tetranychus urticae Koch, allowing selection of six hybrids (two susceptible and four resistant) for each generation of each family. When these 24 hybrids were characterized for spider mite repellency with thumbtack bioassays, two hybrids had repellent leaflets, demonstrating that repellency was genetically transferred to interspecific tomato hybrids. Leaflet washes containing trichome secretions from each of three hybrids, including the two having repellent leaflets, were repellent in bridge bioassays. For the two hybrids having repellent leaflets and leaflet washes, removal of trichome secretions by dipping leaflets in methanol eliminated leaflet repellency. 2,3-Dihydrofarnesoic acid was present in trichome secretions of the hybrids having leaflet repellency, and it also was present in secretions of other hybrids, indicating that its presence is essential, but not sufficient for leaflet repellency. With regard to resistance, 16 of the hybrids tested had been identified as resistant in a whole leaf bioassay, but only two had repellent leaflets, indicating that other mechanisms of resistance are present in the resistant L. hirsutum parent.  相似文献   

6.
To quantify the damage caused by twospotted spider mite, Tetranychus urticae Koch, feeding on cucumber, Cucumis sativus L., leaf-cell and -tissue damage was assessed. On the abaxial leaf surface, adult T. urticae could feed through the spongy parenchyma and part of the palisade parenchyma of the leaf, while immature T. urticae could feed only through the sponge parenchyma. T. urticae punctured individual epidermal cells and consumed the contents of the mesophyll cells. Injured leaves had more empty space in the spongy parenchyma and fewer chloroplasts per cell. Damage also occurred even in the adjacent uninjured parenchyma cells without additional T. urticae feeding injury. Net photosynthetic rate, total chlorophyll content, and greenness of the leaf were significantly affected by feeding as quantified by mite-days. The percent loss of these parameters increased linearly or nonlinearly as mite-days increased, regardless of mite developmental stages. At 1,000 mite-days per 6 cm2, net photosynthetic rate was reduced by approximately 50 and 95%, total chlorophyll content was reduced by approximately 55 and 80%, and greenness was reduced by approximately 50 and 80% by feeding by immature and adult T. urticae, respectively.  相似文献   

7.
We focused on the influence of different temperature amplitudes on development and reproduction of the two-spotted spider mite, Tetranychus urticae Koch, at a 16:8 (L:D) h photoperiod and 60–95 % RH. The temperature amplitudes varied from 0 to 24 °C in steps of 6 °C; i.e. 22 ± 0, 22 ± 3, 22 ± 6, 22 ± 9 and 22 ± 12 °C. Temperature changed every 24 h between a low and an upper value, but without changing the average temperature (22 °C). The number of eggs laid by five females for 24 h was slightly lower at 22 ± 12 °C than at constant temperature (22 ± 0 °C), and egg hatchability differed among the five temperature regimes. Developmental time at 22 ± 0 °C was shorter than that at 22 ± 3 and 22 ± 6 °C, but longer than that at 22 ± 9 and 22 ± 12 °C. The oviposition period, total fecundity per female and adult longevity gradually decreased with increasing amplitudes. Sex ratio was similar at all five temperature regimes. The intrinsic rate of natural increase (r m) was affected by temperature amplitude and the r m-values at all amplitudes except 22 ± 12 °C were higher than that at constant temperature. Thus, this study showed that variable temperature regimes influence population growth rates of T. urticae and that large amplitude regimes are stressful for this species.  相似文献   

8.
The effect of imidacloprid on fecundity in twospotted spider mites, Tetranychus urticae Koch, was investigated in laboratory experiments using individual females on bean leaf discs. Mites were directly exposed to spray formulations of imidacloprid or fed on discs cut from a systemically treated bean plant. Imidacloprid-treated T. urticae produced 10-26% more eggs during the first 12 d of adult life and 19-23% more during adulthood compared with a water-only treatment. Increased egg production occurred immediately after exposure and lasted for about 15 d in sprayed mites. In mites exposed to imidacloprid by ingestion, increased egg production was not apparent until after 6 d and lasted until about day 18. Longevity was significantly greater in mites that ingested imidacloprid but not in sprayed mites. The significance and importance of imidacloprid-stimulation of fecundity in T. urticae to pest management in crop systems like hops, which routinely use this insecticide, is discussed.  相似文献   

9.
10.
In mites, vitellogenin synthesis, regulation and uptake by the oocytes as vitellin remain practically unknown. Although a partial sequence of the gene is now available, no previous studies have been conducted that describe the native vitellin protein in mites. The objective of this study was to characterize vitellin in the twospotted spider mite, Tetranychus urticae. The native twospotted spider mite vitellin migrated as a single major band with a molecular weight of 476 ± 14.5 kDa as compared to 590 ± 25.5 kDa for vitellin from the American dog tick, Dermacentor variabilis. However, isoelectric focusing analysis of native spider mite vitellin showed five bands with pI values slightly acidic to neutral (pH 5.8, 6.2, 6.7, 7.0 and 7.2), as is the case for insect and tick vitellins. Reducing conditions (SDS-PAGE) also revealed multiple subunits ranging from 290.9 to 3.6 kDa and was similar to that found in D. variabilis. Spider mite vitellin weakly bound lipids and carbohydrates compared to the tick. Unlike D. variabilis, the spider mite egg yolk protein does not bind heme. The significance of non-heme binding in mites is discussed.  相似文献   

11.
12.
Adult female twospotted spider mites, Tetranychus urticae Koch, reared on lima bean plants were moved to cucumber, maize, or new lima bean plants (the latter being a control) and evaluated after 24 h or 7 d for changes in susceptibility to three pesticides and in levels of related detoxification enzymes. The largest and most consistent changes were observed in mites feeding on cucumber. Susceptibility of mites on cucumber to the synthetic pyrethroids bifenthrin and lambda-cyhalothrin was greater than that of mites reared on lima bean and maize after only 24 h on the plants, and remained higher after 7 d. Mites on cucumber also were more susceptible to the organophosphate dimethoate than were mites on lima bean, but only after 7 d on the host. Susceptibility was inversely related to activities of both general esterase and glutathione S-transferase (GST) in mites on cucumber; general esterase and GST activities were 60 and 25% lower, respectively, than activities of twospotted spider mite on lima bean after 7 d of feeding. Mites on maize were slightly but significantly more susceptible than those on lima bean to bifenthrin, but not to lambda-cyhalothrin, after 7 d and to dimethoate after 24 h but not after 7 d. General esterase and GST activities in twospotted spider mite fed on maize for 24 h were 20 and 16% higher, respectively, than activities in twospotted spider mite on lima bean, but general esterase activity was 30% lower than lima bean-fed mites and GST was not different after 7 d. Thus, plant-induced changes in general esterase activity, perhaps in combination with GST activity, in twospotted spider mite appear to be inversely related to, and possibly responsible for, changes in susceptibility of twospotted spider mite to several pesticides, particularly the synthetic pyrethroids. General esterases appear to play less of a role in the detoxification of the organophosphate insecticide dimethoate.  相似文献   

13.
Efficacy of rosemary, Rosmarinus officinalis L., essential oil was assessed against twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), as well as effects on the tomato, Lycopersicum esculatum Mill., host plant and biocontrol agents. Laboratory bioassay results indicated that pure rosemary oil and EcoTrol (a rosemary oil-based pesticide) caused complete mortality of spider mites at concentrations that are not phytotoxic to the host plant. The predatory mite Phytoseiulus persimilis Athias-Henriot is less susceptible to rosemary oil and EcoTrol than twospotted spider mite both in the laboratory and the greenhouse. Rosemary oil repels spider mites and can affect oviposition behavior. Moreover, rosemary oil and rosemary oil-based pesticides are nonpersistent in the environment, and their lethal and sublethal effects fade within 1 or 2 d. EcoTrol is safe to tomato foliage, flowers, and fruit even at double the recommended label rate. A greenhouse trial indicated that a single application of EcoTrol at its recommended label rate could reduce a twospotted spider mite population by 52%. At that rate, EcoTrol did not cause any mortality in P. persimilis nor did it affect their eggs. In general, EcoTrol was found to be a suitable option for small-scale integrated pest management programs for controlling twospotted spider mites on greenhouse tomato plants.  相似文献   

14.
There was no difference in the direct toxicity of fluvalinate and esfenvalerate to twospotted spider mite (TSSM), Tetranychus urticae Koch. adults. The residual toxicity LC50 of esfenvalerate was lower. Neither pyrethroid was toxic (<10% mortality) to TSSM eggs or adults at their recommended field concentrations. Fluvalinate was twice as toxic (45% mortality) than esfenvalerate to TSSM larvae at 0.01 g.a.i L-1. The toxicity of the pyrethroids to TSSM protonymphs and deutonymphs was similar (16–28% mortality at 0.1 g a.i. L-1). Dispersal from the treated surface was the main response to both pyrethroids by TSSM protonymphs, deutonymphs and adults. Maximum run-off by TSSM adults from fluvalinate and esfenvalerate treated surfaces was found with 0.01 and 0.005 g a.i. L-1 respectively. Spin-down from pyrethroid treated surfaces was positively correlated with concentration. Oviposition was negatively correlated with concentration. Fluvalinate caused greater reductions in oviposition than esfenvalerate. Both pyrethroids reduced TSSM development rate from larval, protonymph and deutonymph stages, but fluvalinate caused larger reductions. Both pyrethroids prevented mating: for ten days oviposition 93% and 98% of offspring were male for esfenvalerate and fluvalinate respectively at 0.1 g a.i. L-1. These findings are discussed with respect to the incidence of pyrethroid induced mite outbreaks.  相似文献   

15.
李定旭  田娟  郭艳兰  张晓宁  杨玉玲 《昆虫学报》2009,52(12):1312-1318
为了探索雌螨延迟交配对整个种群的负面效应, 采用叶碟饲养法, 在室内条件下研究了延迟交配对山楂叶螨Tetranychus viennensis Zacher繁殖及种群增长的影响。结果表明: 雌螨延迟交配主要影响寿命、产卵量, 进而影响种群增长率; 随着雌螨交配时间的延迟, 影响的程度逐渐加剧。交配延迟达到或超过7 d, 雌螨的寿命比对照延长20.17%, 产卵量比对照下降26.74%, 但对后代的孵化率、存活率无明显影响。对生命表参数的分析结果表明, 雌螨交配延迟达到或超过7 d, 会导致净生殖率显著下降、平均世代周期历期显著延长, 而内禀增长率则在交配延迟3 d时显著降低。未经交配的雌螨只能营孤雌生殖, 寿命延长了31.14%, 产卵量减少了30.08%。营孤雌生殖的雌螨可以通过与其后代回交而实现两性生殖。结果提示延迟交配会导致山楂叶螨的繁殖力降低。  相似文献   

16.
The twospotted spider mite, Tetranychus urticae Koch, is an important pest of impatiens, a floricultural crop of increasing economic importance in the United States. The large amount of foliage on individual impatiens plants, the small size of mites, and their ability to quickly build high populations make a reliable sampling method essential when developing a pest management program. In our study, we were particularly interested in using spider mite counts as a basis for releasing biological control agents. The within-plant distribution of mites was established in greenhouse experiments and these data were used to identify the sampling unit. Leaves were divided into three zones according to location on the plant: inner, intermediate, and other. On average, 40, 33, and 27% of the leaves belonged to the inner, intermediate, and other leaf zones, respectively. However, because 60% of the mites consistently were found on the intermediate leaves, intermediate leaves were chosen as the sampling unit. These results lead to the development of a presence-absence sampling method for T. urticae by using Taylor coefficients generic for this pest. The accuracy of this method was verified against an independent data set. By determining numerical or binomial sample sizes for consistently estimating twospotted spider mite populations, growers will now be able to determine the number of predatory mites that should be released to control twospotted spider mites on impatiens.  相似文献   

17.
Experimental observations on the arrenotokous reproductive patterns of two spider mite species (Acari: Tetranychidae), the long-seta form of Schizotetranychus celarius (Banks ) and Tetranychus urticaeKoch , revealed that reproduction of unfertilized females of the former is very differnt from that of the latter. Unfertilized females of S. celarius, which has a subsocial life, laid a few eggs and then became inactive. In contrast, the fecundity of unfertilized T. urticae females was only slightly reduced as compared with fertilized females. Mother-son matings may thus sometimes occur in natural S. celarius populations. A two-year field survey revealed that, in the absence of males, overwintering females of S. celarius occasionally remain unfertilized until early spring. Furthermore, nest foundation observed in late spring indicated that most of the season's first nests were founded by single females. These two sets of observations strongly suggest that motherson mating takes places in nature, corresponding to the reproductive trait seen in the experiment. Mother-son mating inevitably increases the relatedness between nest members. The estimated father's relatedness to its offspring is extraordinarily high under such condition. The possibility that kin-selection in the long seta-form of S. celarius led to subsociality, especially paternal care, is suggested.  相似文献   

18.
The twospotted spider mite, Tetranychus urticae Koch, is an important pest of ivy geranium and other ornamental plants. As a part of our long-term goal to develop an integrated crop management program for ivy geraniums, the focus of this study was to produce a reliable sampling method for T. urticae on this bedding plant. Within-plant mite distribution data from a greenhouse experiment were used to identify the young-fully-opened leaf as the sampling unit. We found that 53% of the mites on a plant are on the young-fully-opened leaves. On average 22, 37, and 41% of the leaves belonged to the young, young-fully-opened, and old leaf categories, respectively. We then developed a presence-absence sampling method for T. urticae in ivy geranium using generic Taylor's coefficients for this pest. We found the optimal binomial sample sizes for estimating populations of T. urticae at densities of between 0 and 3 mites/leaf to be quite large; therefore, we recommend the use of numerical sampling within this range of T. urticae densities. We also suggest that population estimates of T. urticae on ivy geranium be done based on mite density/unit area of greenhouse space, both for conventional greenhouse pest management, and for determining how many phytoseid predators to release when using biological control.  相似文献   

19.
Bifenthrin (Talstar®) was registered at the beginning of the 1993/94 season for control of Helicoverpa spp. and Tetranychus urticae in Australian cotton. Resistance was monitored in T. urticae following registration but despite the implementation of resistance management, resistance evolved in the 1996/97 cotton-growing season after only four seasons use. During the following three seasons resistance increased progressively both in level (from 1.2- to 109-fold at LC50) and abundance (from 20 to 90% strains) and was linked to field control failures. The evolution of resistance has now reduced the reliability of bifenthrin for T. urticae control in Australian cotton. The impact of bifenthrin resistance in T. urticae and its continued management in Australian cotton is discussed.  相似文献   

20.
The responses of Tetranychus urticae Koch from Australian cotton to chlorfenapyr has been monitored since the 1997–1998 growing season. Resistance was first detected in the 2001–2002 season and then increased quickly in both level and proportion of resistant strains detected. In response, the resistance management strategy for chlorfenapyr use in cotton was altered and now recommends a further restriction of use from two to one spray per season. There was no evidence of negative cross-resistance to the pyrethroid bifenthrin, but chlorfenapyr was associated with an undefined negative cross-resistance.  相似文献   

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