Effect of Aedes aegypti (Diptera:Culicidae) Age on Sex Ratios in Romanomermis culicivorax (Nematoda:Mermithidae)

First, second, third, early and late fourth-instar larvae, and pupae of Aedes aegypti were infected with Romanomerrais culicivorax and reared at 20, 25, and 30 C. An increase in the ratio of male to female nematodes was observed with increase in host age at the time of infection at each temperature. The number of pupal and late fourth-instar infections was low, but R. culicivorax continued to develop in adult A. aegypti. Since male nematodes were recovered from both male and female hosts infected as late fourth instars or pupae, the sex of the host did not influence the sex of the nematode.     

Parasitoid-induced behavioral alterations of Aedes aegypti mosquito larvae infected with mermithid nematodes (Nematoda: Mermithidae).

A wide range of parasites are known to cause behavioral changes in their hosts and parasitized insects are especially amenable to the study of such changes. The majority of studies addressing parasite-induced behavioral alterations have focused on parasites with complex life cycles and the adaptive nature of such changes. Behavioral changes caused by parasitoids, single-host parasites that kill their host upon emergence, have been studied less and the adaptive nature of these changes is likely to be different than those in complex life cycles. I investigated behavioral alterations in Aedes aegypti mosquito larvae infected with parasitoid nematodes (family Mermithidae). I conducted several experiments in which I tested the following hypotheses: 1) Mermithid nematodes induce behavioral changes in mosquito larvae and the changes are density dependent. 2) Different species of mermithid nematodes induce similar changes in mosquito larvae behavior. 3) Behavioral alterations vary with mermithid developmental stage. 4) Mosquito larvae infected with mermithid nematodes behave similarly to uninfected food-deprived mosquito larvae. I found that 4th instar Ae. aegypti infected with Romanomermis culicivorax or Strelkovimermis spiculatus exhibited resting behaviors significantly more often than uninfected controls but that intensity of infection did not affect activity levels. In earlier instars, infected mosquito larvae were more active than uninfected control larvae in some behaviors associated with feeding. There was no significant difference between infected and uninfected food-deprived mosquitoes in nine of the ten behaviors observed. The decrease in activity of late instar Ae. aegypti larvae infected with mermithids may be a parasitoid adaptation that reduces the risk of predation and thus increases host and parasitoid survival. The increase in feeding activity in earlier instars as well as the similarity between uninfected food-deprived and infected Ae. aegypti behavior may indicate that these behaviors are adaptive for the parasitoid, increasing nutritional acquisition for successful parasitoid development.     

Susceptibility of the Carrot Weevil (Coleoptera: Curculionidae) to Steinernema feltiae,S. bibionis,and Heterorhabditis heliothidis

Larvae, pupae, and adults of the carrot weevil (Listronotus oregonensis) were infected and killed by the three entomophagous nematodes (Steinernema feltiae, S. bibionis, and Heterorhabditis heliothidis) under controlled conditions. Third-stage larvae were more susceptible than pupae or adults. S. feltiae and S. bibionis were the most aggressive nematode species, causing larval mortality after 24-48 hours in both continuous and 2-hour contact with nematode suspension. The nematodes multiplied sufficiently in all insects at all stages of development; however, production of infective-stage larvae per host cadaver was variable.     

Parasitism and the burrowing depth of the beach hopper Talorchestia quoyana (Amphipoda: Talitridae)

Talitrid amphipods spend their days burrowed in sand to avoid predators as well as desiccation and heat stress, although other factors may influence burrowing depth. We investigated the potential role of mermithid nematode parasites in determining burrowing depth in the amphipod Talorchestia quoyana. Mermithids grow as parasites inside amphipods until they reach adulthood, when they must emerge from their host into moist sand to complete their life cycle and reproduce. When allowed to burrow to a depth of their choice in experimental situations, large amphipods burrowed deeper than small ones. In addition, deep-burrowing amphipods were more likely to be infected by mermithid nematodes, and harboured longer worms, on average, than amphipods that burrowed close to the sand surface. This last result is not an artefact of the larger size of deep-burrowing amphipods: the increase in worm length with increasing depth was found after statistical correction for host size. In other words, amphipods that burrowed deeper harboured longer worms than expected based on their body size, whereas those that stayed near the surface of the sand column harboured worms shorter than one would expect based on host size. This implies that the greater burrowing depth of infected amphipods is a consequence, and not a cause, of infection. These results emphasize the importance of parasitism as a determinant of the small-scale spatial distribution of their hosts.     

Influence of Lactic Acid Bacteria on Longevity of Caenorhabditis elegans and Host Defense against Salmonella enterica Serovar Enteritidis

This study aimed to develop a convenient model to investigate the senescence of host defenses and the influence of food and nutrition. A small soil nematode, Caenorhabditis elegans, was grown for 3 days from hatching on a lawn of Escherichia coli OP50 as the normal food source, and subsequently some of the nematodes were fed lactic acid bacteria (LAB). The life spans of worms fed LAB were significantly longer than the life spans of those fed OP50. To investigate the effect of age on host defenses, 3- to 7-day-old worms fed OP50 were transferred onto a lawn of Salmonella enterica serovar Enteritidis for infection. The nematodes died over the course of several days, and the accumulation of salmonella in the intestinal lumen suggested that the worms were infected. The 7-day-old worms showed a higher death rate during the 5 days after infection than nematodes infected at the age of 3 days; no clear difference was observed when the worms were exposed to OP50. We then investigated whether the LAB could exert probiotic effects on the worms' host defenses and improve life span. Seven-day-old nematodes fed LAB from the age of 3 days were more resistant to salmonella than worms fed OP50 until they were infected with salmonella. This study clearly showed that LAB can enhance the host defense of C. elegans and prolong life span. The nematode appears to be an appropriate model for screening useful probiotic strains or dietetic antiaging substances.     

Effect of Soil Texture on the Distribution and Infectivity of Neoaplectana carpocapsae (Nematoda: Steinernematidae)

The vertical migration of N. carpocapsae infective juveniles applied to the soil surface or introduced 14 cm below the soil surface was studied in four different soil types (pure silica sand, coarse sandy loam, silty clay loam, and clay). The percentage of juveniles able to migrate and infect wax moth pupae placed in the soil decreased as the percentage of clay and silt increased. Most nematodes placed on the soil surface remained within 2 cm of the surface, but some penetrated to a depth of 10 cm in pure silica sand and coarse sandy loam to infect pupae. Some pupae at the same depth were also infected with nematodes in silty clay loam soil. In pure silica sand and coarse sandy loam, nematodes introduced 14 cm below the soil surface were able to infect wax moth pupae located between 4 and 24 cm. Movement was least in clay soil and limited in silty clay loam. Nematodes showed a tendency to disperse upwards from the point of application. In all cases the number of migrating nematodes was greatest when wax moth pupae were present.     

New record of Ascaridia nymphii (Secernentea: Ascaridiidae) from macaw parrot,Ara chloroptera,in China

Present study was performed to identify the species of ascarids from macaw parrot, Ara chloroptera, in China. Total 6 ascarids (3 males and 3 females) were collected in the feces of 3 macaws at Guangzhou Zoo in Guangdong Province, China. Their morphological characteristics with dimensions were observed under a light microscope, and their genetic characters were analyzed with the partial 18S rDNA, ITS rDNA and nad4 gene sequences, respectively. Results showed that all worms have no interlabia but male worms have two alate spicules, well-developed precloacal sucker and a tail with ventrolateral caudal alae and 11 pairs of papillae. The partial 18S rDNA, ITS rDNA and nad4 sequences were 831 bp, 1015 bp and 394 bp in length, respectively. They showed the highest similarity of 99.8% (18S rDNA) with Ascaridia nymphii, 93.8% identities (ITS rDNA) with A. columbae and 98.5% to 99.5% identities (nad4) with Ascaridia sp. from infected parrot. All Ascaridia nematodes from the macaws were clustered into one clade and formed monophyletic group of Ascaridia with A. columbae and A. galli in two phylogenetic trees. It is observed that the combining morphological and sequencing data from three loci, the present Ascaridia species was identified as Ascaridia nymphii, which is the first record of A. nymphii from macaw parrot in China.     

Infochemicals influencing the host foraging behaviour of Dahlbominus fuscipennis, a pupal parasitoid of the European spruce sawfly (Gilpinia hercyniae)

The chalcidoid wasp Dahlbominus fuscipennis Zett. (Hymenoptera: Eulophidae) parasitises pupae of the European spruce sawfly Gilpinia hercyniae Htg. (Hymenoptera: Diprionidae). In this parasitoid-host system the role of infochemicals for successful parasitisation was studied. Olfactometer experiments and dual choice bioassays in petri dishes showed that D. fuscipennis was not attracted by volatiles from a plant-host complex consisting of spruce, sawfly larvae and pupae. On the other hand, odours from needles of Picea abies (L.), that were collected from the ground, attracted D. fuscipennis to the habitat of the sawfly. Volatiles from host pupae were not attractive, whereas a contact kairomone from sawfly pupae elicited frequent antennal drumming, a behaviour that indicates host recognition. This contact kairomone was extracted with methanol from the outer cocoon layer of the pupa. The kairomone is not produced by the silk glands of the last instar larva spinning the cocoon, since a methanolic extract of silk gland secretion did not elicit any response in D. fuscipennis. Further bioassays revealed that non-volatile components of the integument of the prepupa within the cocoon also elicited antennal drumming. We suggest that chemicals of the prepupal integument diffuse from inside the cocoon to the outside where they inform the parasitoid on the suitability of the host.     

Spread and Transmission of Bacterial Pathogens in Experimental Populations of the Nematode Caenorhabditis elegans

Caenorhabditis elegans is frequently used as a model species for the study of bacterial virulence and innate immunity. In recent years, diverse mechanisms contributing to the nematode''s immune response to bacterial infection have been discovered. Yet despite growing interest in the biochemical and molecular basis of nematode-bacterium associations, many questions remain about their ecology. Although recent studies have demonstrated that free-living nematodes could act as vectors of opportunistic pathogens in soil, the extent to which worms may contribute to the persistence and spread of these bacteria has not been quantified. We conducted a series of experiments to test whether colonization of and transmission between C. elegans nematodes could enable two opportunistic pathogens (Salmonella enterica and Pseudomonas aeruginosa) to spread on agar plates occupied by Escherichia coli. We monitored the transmission of S. enterica and P. aeruginosa from single infected nematodes to their progeny and measured bacterial loads both within worms and on the plates. In particular, we analyzed three factors affecting the dynamics of bacteria: (i) initial source of the bacteria, (ii) bacterial species, and (iii) feeding behavior of the host. Results demonstrate that worms increased the spread of bacteria through shedding and transmission. Furthermore, we found that despite P. aeruginosa''s relatively high transmission rate among worms, its pathogenic effects reduced the overall number of worms colonized. This study opens new avenues to understand the role of nematodes in the epidemiology and evolution of pathogenic bacteria in the environment.     

Susceptibility of the Mediterranean fruit fly (Ceratitis capitata) and the Natal fruit fly (Ceratitis rosa) to entomopathogenic nematodes

The potential of entomopathogenic nematodes, Heterorhabditis bacteriophora, Heterorhabditis zealandica and Steinernema khoisanae, to infect pupariating larvae, pupae and adults of Ceratitis capitata and Ceratitis rosa was investigated in laboratory bioassays. Pupariating larvae and adult flies were susceptible to nematode infection, with no infection recorded for the pupae. Pupariating larvae of C. capitata were generally more susceptible to infection than those of C. rosa. Significantly more larvae of C. capitata were infected by H. bacteriophora. For C. rosa, highest infectivity of larvae was obtained with H. zealandica. In contrast, adults of both species were highly infected by S. khoisanae.     

Effect of Soil Texture on the Distribution and Infectivity of Neoaplectana glaseri (Nematoda: Steinernematidae)

The vertical migration of infective juveniles of Neoaplectana glaseri applied to the soil surface or introduced 16 cm below the soil surface was studied in pure silica sand, coarse sandy loam, silty clay loam, and clay. The number of juveniles that migrated and infected wax moth pupae placed in the soil decreased as the proportion of clay and silt increased. The majority of nematodes moved downwards 2-6 cm from the surface, but some penetrated to a depth of 14 cm in pure silica sand and coarse sandy loam. In pure silica sand and coarse sandy loam, nematodes introduced 16 cm below the soil surface were able to infect wax moth pupae located at depths of 0-4 cm and 28-32 cm. Nematodes showed a greater tendency to disperse downwards from the point of application. Movement of the nematode was least in clay soil and limited in silty clay loam soil. The number of migrating nematodes was greatest when wax moth pupae were present.     

Influence of lactic acid bacteria on longevity of Caenorhabditis elegans and host defense against salmonella enterica serovar enteritidis

This study aimed to develop a convenient model to investigate the senescence of host defenses and the influence of food and nutrition. A small soil nematode, Caenorhabditis elegans, was grown for 3 days from hatching on a lawn of Escherichia coli OP50 as the normal food source, and subsequently some of the nematodes were fed lactic acid bacteria (LAB). The life spans of worms fed LAB were significantly longer than the life spans of those fed OP50. To investigate the effect of age on host defenses, 3- to 7-day-old worms fed OP50 were transferred onto a lawn of Salmonella enterica serovar Enteritidis for infection. The nematodes died over the course of several days, and the accumulation of salmonella in the intestinal lumen suggested that the worms were infected. The 7-day-old worms showed a higher death rate during the 5 days after infection than nematodes infected at the age of 3 days; no clear difference was observed when the worms were exposed to OP50. We then investigated whether the LAB could exert probiotic effects on the worms' host defenses and improve life span. Seven-day-old nematodes fed LAB from the age of 3 days were more resistant to salmonella than worms fed OP50 until they were infected with salmonella. This study clearly showed that LAB can enhance the host defense of C. elegans and prolong life span. The nematode appears to be an appropriate model for screening useful probiotic strains or dietetic antiaging substances.     

Persistent infection with Strongyloides venezuelensis in the Mongolian gerbil (Meriones unguiculatus)

To examine the fate of Strongyloides venezuelensis. Mongolian gerbils (Meriones unguicalatus) were orally infected with 1,000 L3 larvae per animal. Altogether, 50 gerbils divided into 5 groups of 10 each were monitored for a period of 570 days to document the kinetics of faecal egg output, adults worm population, morphological development, fecundity, and hematological changes including peripheral blood eosinophilia. This study chronicled a life long parasitism of S. venezuelensis in the gerbil host, and showed that S. venezuelensis infection was quite stable throughout the course of infection and the worms maintained their normal development as evidenced by their body dimension. A progressive loss of body condition of the infected gerbils was observed as the level of infection advanced. However, no detectable pathological changes were observed in the gastrointestinal tract. The present findings indicate that an immunocompetent host, such as the Mongolian gerbil, can serve as a life long carrier model of S. venezuelensis if the worms are not expelled within 570 days after infection.     

Larval anisakids and other helminths in the hake, Merluccius gayi (Guichenot) from Chile

One hundred and twenty hake Merluccius gayi caught off central Chile were examined for helminth parasites. Sixty hake were kept in ice from the catch of a trawler were examined upon arrival at port, while the other 60 hake were dissected on board immediately after collection. Ninety one per cent of hake were infected with one or more species of the following helminths: trypanorhynch plerocerci of Grillotia dollfusi Carvajal, 1971; adult worms of the genus Clestobothrium; larval nematodes of the genera Anisakis and Phocanema; 86% of the total were infected with Anisakis sp. and 42.5% with Phocanema sp. Infection with larval nematodes increased with length of the fish and there was a greater incidence of infection among the females. There was a greater frequency of occurrence of the parasites in the viscera than in the musculature.     

Larval nematodes (Spiruroidea: Cystidicolidae) in Octopus vulgaris (Mollusca: Cephalopoda: Octopodidae) from the northeastern Atlantic Ocean.

Larval nematode parasites (Spiruroidea: Cystidicolidae) are recorded for the first time in Octopus vulgaris Cuvier, 1797 in the northeastern Atlantic Ocean. Prevalence was 16% and mean intensity was 1.46 worms/host. Body length of larval nematodes ranges from 8.3 to 9.3 mm, with a distance from the anterior end to nerve ring from 187.5 to 200 microm, and to excretory pore 194.6-350 microm. Anatomical characteristics, such as deirid, nerve ring, cephalic alae, excretory pore, pseudolabia amphids, sclerotized protuberance, and anus, examined using light microscopy (LM) or scanning electron microscopy (SEM), are illustrated. The nematode was designed as a cystidicolid "Type A" larva. The hemocytic infiltration present in the host tissue around the nematode capsule and the mechanical compression in the infected organs denote the pathogenicity of this nematode. In the study area, O. vulgaris may play the role of an intermediate or paratenic host in the nematode life cycle.     

Phenotypic plasticity in adult worms of Schistosoma mansoni (Trematoda:Schistosomatidae) evidenced by brightfield and confocal laser scanning microscopies

A comparative morphometric study was performed to identify host-induced morphological alterations in Schistosoma mansoni adult worms. A wild parasite population was obtained from a naturally infected rodent (Nectomys squamipes) and then recovered from laboratory infected C3H/He mice. Furthermore, allopatric worm populations maintained for long-term under laboratory conditions in Swiss Webster mice were passed on to N. squamipes. Suckers and genital system (testicular lobes, uterine egg, and egg spine) were analyzed by a digital system for image analysis. Confocal laser scanning microscopy (CLSM) showed details of the genital system (testicular lobes, vitelline glands, and ovary) and the tegument just below the ventral sucker. Significant morphological changes (p < 0.05) were detected in male worms in all experimental conditions, with no significant variability as assessed by CLSM. Significant changes (p < 0.05) were evident in females from the wild population related to their ovaries and vitelline glands, whereas allopatric females presented differences only in this last character. We conclude that S. mansoni worms present the phenotypic plasticity induced by modifications in the parasite's microenvironment, mainly during the first passage under laboratory conditions.     

Hymenolepis nana: Survival in the immunized mouse

Mice initially infected with Hymenolepis nana eggs became completely immune to challenge with mouse-derived cysticercoids (cysts) after more than 10 days. The host possessed at least two separated immune responses, one directed exclusively against reinfection with eggs (early response) and the other against cyst infection (late response). In two different mouse strains the responses showed markedly different duration both for the time lag prior to acquisition of the late response and for the survival of the initially infected worms, but were otherwise similar. The mice became immune to adult tapeworms and expelled the initially infected, destrobilated worms; this third immune response determines the longevity of H. nana in the mouse host. Thus, there is a strong indication that H. nana successively changes its immunogenicity during development, each stage stimulating immunity after a time lag. It is possible that the longevity of H. nana in a mouse strain depends on the length of time prior to acquisition of immune responses directed not against the tissue stage (early response), but against the lumen stages (late response and worm expulsion response).     

Mating and Sexual Communication by Steinernema carpocapsae (Nemata: Steinernematidae)

Entomopathogenic nematodes are lethal insect parasites that reproduce exclusively inside their hosts in nature. Infection decisions made by the free-living infective-stage juveniles have an impact on reproductive success, but it is likely that mating decisions are made by adults while inside their host. We investigated sexual communication between male and female adult stages of Steinernema carpocapsae (Rhabditida: Steinernematidae) to assess whether mating is chemically mediated during the adult stage or results from incidental encounters between adults inside the insect host. To assess chemical communication, we measured the behavioral response of adult male S. carpocapsae to several different potential sources of chemical information. Male S. carpocapsae responded to virgin females only and were not influenced by mated conspecific females, conspecific males, or heterospecific females. These results show that species-specific communication takes place between adult entomopathogenic nematodes within the host cadaver just prior to mating.     

Biology of Thripinema nicklewoodi (Tylenchida), an Obligate Frankliniella occidentalis (Thysanoptera) Parasite

Frankliniella occidentalis, a serious pest of agricultural crops, is difficult to manage because chemical and biological control measures frequently fail to affect F. occidentalis in their preferred microhabitats. Parasitism by the host-specific, entomopathogenic nematode Thripinema nicklewoodi may provide a much-needed alternative to current control strategies. Infection does not cause death of the host; rather, the result is sterilization that leads to suppression of F. occidentalis populations. We describe a simple rearing method and the results from studies aimed at providing details on its biology-both essential first steps to examining its biological control potential. All F. occidentalis life stages are susceptible to infection, but to varying degrees (most susceptible to least susceptible): female pupae, second instar larvae, first instar larvae, male pupae, adult females, adult males. Nematodes emerge from female and male F. occidentalis for approximately 15 and 9 days, with approximately 14 and 7 nematodes emerging per day, respectively. Females and males are short-lived outside of the host, with mean survival rates ranging between 7 and 86 hours. Transmission does not occur in the soil but rather on or within plant structures that are preferred microhabitats visited by F. occidentalis. Results from a dose-response study suggest that augmentative applications of T. nicklewoodi may be useful to generate increased infection rates and subsequent suppression of F. occidentalis populations.