丛枝菌根（AM）生物技术在现代农业体系中的生态意义

菌根是植物根系与特定的土壤真菌形成的共生体,有利于生态系统中养分循环,协助植物抵御不良环境胁迫．自然条件下,大多数植物表现一定的菌根依赖性,在植株根系发育过程中如能与适宜的菌根真菌形成良好的菌根结构,可提高产量,改善品质,其中丛枝菌根是最普遍的类型．丛枝菌根帮助植物抵御不良环境胁迫及病虫害,促进植物健康生长,可减少化学肥料、杀虫剂施用量,以减少对环境、生态不利的化学物质施用量．丛枝菌根共生体可加速根系生长,提高对移动性低的无机离子吸收,加速养分循环利用,增强植物对不良胁迫(生物与非生物)因素的耐受力,形成良好的土壤结构,提高植物群体的多样性．文章综述了丛枝菌根真菌生态特征,丛枝菌根对寄主植物的影响,丛枝菌根生物技术应用于农业体系的生态意义及其应用潜力．     

鄱阳湖流域长期施肥下双季稻田的土壤基础地力

为探讨鄱阳湖流域不同施肥措施的土壤基础地力变化规律,基于长期施肥定位试验(始于1981年),采集定位30年时的不施肥(CK)、施用氮磷钾肥(NPK)和氮磷钾肥与有机肥配施(NPKM)土壤,于2012—2014年进行连续3年的施肥和不施肥试验,分析水稻产量和土壤基础地力贡献率的差异及关键影响因子.结果表明:不论施肥与否,3年的水稻产量均表现出NPKM处理显著高于NPK和CK处理,尤其是在不施肥条件下,NPKM处理的年产量分别比CK和NPK处理增加37.7%～143.9%和20.8%～66.7%.3年间,CK、NPK和NPKM处理的土壤基础地力贡献率分别为41.8%～53.1%、45.2%～62.6%和59.1%～88.1%,且NPKM处理的土壤基础地力贡献率均显著高于NPK和CK处理.进一步分析发现,土壤有机质和有机碳平衡量与土壤基础地力贡献率呈显著正相关.总之,在鄱阳湖流域的双季稻田,长期有机无机肥配施有利于该地区土壤基础地力的提升,且土壤有机质和有机碳平衡是土壤基础地力变化的关键因子.     

黄土高原生态工程实施下基于日光诱导叶绿素荧光的植被恢复生产力效益评价

基于日光诱导叶绿素荧光(SIF)揭示黄土高原大面积实施生态工程后植被恢复区域的植被生产力效益。通过分析遥感观测的地表绿度特征变化和土地利用动态, 该研究首先识别了近20年来黄土高原植被恢复区域和原有植被的空间分布范围, 在此基础上, 使用SIF和气象数据, 根据改进机理光响应模型(rMLR)计算了植被总初级生产力(GPP), 对比分析了植被恢复区域绿度特征变化下GPP的差异。结果显示: 空间上, 由于生态工程的广泛实施, 黄土高原整体绿化情况得到了明显改善。在2001-2020年间, 黄土高原上林地恢复面积约3.5万km², 占区域总面积的7.42%; 草地恢复面积11万km², 约占区域总面积的25.25%。整体上, 林地恢复区域的光合能力和生产力水平均低于原有林地, 而草地恢复区域较原有草地高; 恢复林地的GPP相当于原有林地GPP的83.86%, 恢复草地的GPP相当于原有草地的121.10%。在相同的叶面积指数(LAI)等级下, 植被恢复区域与原有植被的生产力呈现不同的差异性, 当LAI较大时植被恢复区域与原有植被的GPP差距较大。由裸地转变而来的植被恢复区域生产力效益最差, 而林地生长区域和退耕还草的植被恢复区域分别是林地恢复和草地恢复中的最优模式。植被恢复区域的LAI增长速率和恢复年限也影响了生产力, LAI增长速率越大的区域生产力效益越高, 林地恢复年限越久越利于生产力的提升, 草地恢复年限较短的区域有较高的生产力。总体上, 由于生态工程的实施, 虽然黄土高原上的植被覆盖面积和生物量得到了显著提高, 但植被生产力(尤其是林地)并没有获得同等程度的恢复, 影响了生态工程的生态效益。     

Assessment of metals in agricultural soil of surrounding areas of Urmia Lake,northwest Iran: A preliminary ecological risk assessment and source identification

The aim of this study was to quantify the levels of toxic heavy metals and sodium in topsoil of farmlands around the Urmia Lake. A total of 96 topsoil samples were collected, pre-treated, and analyzed for metals using ICP-AES.

Median concentrations of As, Cd, Cu, Ni, Pb, Zn, and Na in sampling sites were 5, 0.26, 30, 40, 13, 84, and 251 mg/kg, respectively. The enrichment factor, geoaccumulation index, and contamination factor of the metals ranked them in the order of: Na > Pb > Cu > As > Cd > Zn > Ni, indicating minor contamination for them except Na and Pb with moderate contamination. Furthermore, the spatial analysis indicated that Cd, Cu, Pb, Ni, and Zn had similar distribution patterns in the north and northwest lands of the lake. Principal component analysis revealed anthropogenic sources for Cd, Cu, Ni, Pb, and Zn in the soil, whereas, sources of As and Na were most likely related to the dust emitting from the dried bed of Urmia Lake and from a cement industry. Potential ecological risk index in 7% of the sampling sites was at serious or considerable pollution level, and Cd and Pb were identified as the main pollutants.     

黄土丘陵区纸坊沟流域近70年农业生态安全评价

在国内外已有研究的基础上,结合黄土丘陵区纸坊沟流域近70a来农业生态经济系统演变过程,参考PSR模型,从生态环境、社会经济和综合功能3方面构建黄土丘陵区流域农业生态安全评价指标体系,基于层次分析法赋权的综合指数评价方法,对纸坊沟流域1938～2005年农业生态安全状况进行评价.结果表明:该流域农业生态安全状况发生了先降后升的变化,1938年处于较安全状况,1958年为极不安全状况,1975年和1985年都处于较不安全状况,1995年处于临界安全状况,2005年属于较安全状况.评价结果表明纸坊沟流域还需要进一步优化农业系统结构,实现农业生态安全.     

基于标准化土壤湿度指数的滦河流域农业干旱评价

干旱是造成农业生产损失的主要灾害之一,及时、准确地监测农业干旱对于降低作物生产损失具有重要意义.本研究基于土壤含水量历史时间序列数据构建了标准化土壤湿度指数,分析了滦河流域2002-2019年干旱时空分布变化规律,并对农业干旱进行定量评价.结果表明:研究期间,滦河流域内大部分栅格单元土壤含水量在时间上以正态分布为主,少...     

不同退耕模式细根(草根)分解过程中C动态及土壤活性有机碳的变化

由于土壤活性有机碳可以在土壤全碳变化之前反映土壤因管理措施和环境引起的微小的变化,又直接参与土壤微生物化学转化过程,对土壤碳平衡和土壤化学、土壤肥力保持具有重要意义。因此,采用原状土芯(intact core)法,探讨了4种退耕还林模式--光皮桦(Betula luminifera)与扁穗牛鞭草(Hemarthria compressa)复合模式、扁穗牛鞭草草地、柳杉(Cryptameria fortunei)人工林、光皮桦人工林细根(草根)分解过程中的C动态以及土壤活性有机碳变化。研究结果表明,各模式细根(草根)中的C表现为净释放,其质量残留率符合单指数模型(P＜0.01)。光皮桦与扁穗牛鞭草复合模式下的土壤微生物量碳(SMBC)、水溶性有机碳(WSOC)、易氧化碳(ROC)、总有机碳(TOC)都大于其他3种模式。4种模式下的SMBC对土壤TOC的贡献分别是1.2%-3.3%、0.7%-1.5%、0.8%-2.2%、0.5%-0.8%;光皮桦与扁穗牛鞭草复合模式的ROC/TOC大于其他3种模式模式;各模式土壤ROC含量与土壤TOC呈极显著正相关关系(P＜0.05)。以上结果显示,与其他人工林相比,光皮桦与扁穗牛鞭草复合模式土壤有机碳活性大、易转化,土壤总有机碳的高低决定了易氧化碳的丰缺。     

137Cs和210Pbex示踪黑土区坡耕地土壤侵蚀对有机碳的影响

通过在野外28.5 hm2的坡耕地上采集土壤样品,定量评价了利用137Cs和210Pbex研究土壤有机碳(SOC)动态的潜力,以探讨东北黑土区土壤侵蚀对土壤有机碳的影响.结果表明:农耕地土壤137Cs、210Pbex和SOC在平面和垂直深度上均具有相似的分布特征.在平面上,尽管受土壤侵蚀沉积的影响,137Cs、210Pbex面积活度及SOC储量变异很大,但它们具有相同的变化趋势.在垂直断面上,侵蚀区137Cs、210Pbex和SOC在0～25 cm耕层内分布均匀,25 cm以下放射性活度减小,SOC含量也相应下降;沉积区0～100 cm深度上137Cs和210Pbex呈现先增加后减小的分布规律,SOC也具有类似的变化特征.农耕地SOC与137Cs、210Pbex呈显著线性相关,表明它们在黑土区农耕地上具有相似的物理运移特征,137Cs和210Pbex可直接用来定量评价黑土侵蚀下SOC的时空分布特点.     

Modeling the interannual variation and response to climate change scenarios in gross and net primary productivity of Pinus elliottii forest in subtropical China

In this study, the BIOME-BGC model, a biogeochemical model, was used and validated to estimate GPP (Gross Primary Productivity) and NPP (Net Primary Productivity) of Pinus elliottii forest in red soil hilly region and their responses to inter-annual climate variability during the period of 1993–2004 and climate change scenarios in the future. Results showed that the average total GPP and NPP were 1941 g C m^?2a^?1 and 695 g C m^?2a^?1, and GPP and NPP showed an increasing trend during the study period. The precipitation was the key factor controlling the GPP and NPP variation. Scenario analysis showed that doubled CO₂ concentration would not benefit for GPP and NPP with less than 1.5% decrease. When CO₂ concentration fixed, GPP responded positively to precipitation change only, and temperature increase by 1.5°C with precipitation increase, while NPP responded positively to precipitation change only. When CO₂ concentration was doubled and climate was changed, GPP and NPP responded positively to precipitation change, and GPP also responded positively to temperature increase by 1.5°C with precipitation change.     

Plant species richness and productivity determine the diversity of soil fungal guilds in temperate coniferous forest and bog habitats

Fungi have important roles as decomposers, mycorrhizal root symbionts and pathogens in forest ecosystems, but there is limited information about their diversity and composition at the landscape scale. This work aimed to disentangle the factors underlying fungal richness and composition along the landscape‐scale moisture, organic matter and productivity gradients. Using high‐throughput sequencing, we identified soil fungi from 54 low‐productivity Pinus sylvestris‐dominated plots across three study areas in Estonia and determined the main predictors of fungal richness based on edaphic, floristic and spatial variables. Fungal richness displayed unimodal relationship with organic matter and deduced soil moisture. Plant richness and productivity constituted the key predictors for taxonomic richness of functional guilds. Composition of fungi and the main ectomycorrhizal fungal lineages and hyphal exploration types was segregated by moisture availability and soil nitrogen. We conclude that plant productivity and diversity determine the richness and proportion of most functional groups of soil fungi in low‐productive pine forests on a landscape scale. Adjacent stands of pine forest may differ greatly in the dominance of functional guilds that have marked effects on soil carbon and nitrogen cycling in these forest ecosystems.     

基于结构方程模型的吴起县农业生态经济系统耦合关系

基于2008年吴起县农户调查资料,利用结构方程模型对吴起县农业生态经济系统耦合关系进行了分析.结果表明：具有相关关系的农业资源和产业态势对系统耦合有一定影响,其影响系数分别为0.14和1.00;农业资源与产业态势之间的相关系数为-0.11,说明吴起县的产业发展并非建立在对农业资源有效利用基础之上,农业资源对经济效益的变化没有直接作用;产业的发展主要建立在对农业资源之外的能量和物质的开发利用上,导致产业态势对经济效益的直接路径系数为-2.95,总的路径系数仅为0.23.从农业生态系统与经济系统的关系及其耦合的角度看,目前研究区农业系统建立在不稳定的、较少依赖于本系统资源的耦合态势下,即在农业生态经济系统良性耦合机制的建立与形成过程中潜伏了较大危机.为此,需要提高牧草等农业资源的利用率,发展林草及其相关产业.     

Modeling the effects of Hurricane Hugo on spatial and temporal variation in primary productivity and soil carbon and nitrogen in the Luquillo Experimental Forest, Puerto Rico

Hurricanes account for much of the spatial and temporal variation in forest productivity and soil organic matter pools in many forest ecosystems. In this study, we used an ecosystem level model, TOPOECO, to simulate the effects of Hurricane Hugo (18 September 1989) on spatial and temporal patterns of gross primary productivity (GPP), net primary productivity (NPP), soil organic carbon (SOC) and nitrogen over the entire Luquillo Experimental Forest (LEF), Puerto Rico, a tropical rainforest. Our simulation results indicated that simulated annual GPP increased by an average of 30% five years after Hugo in the Tabonuco forest at low elevations where there was a fast recovery of the canopy, whereas simulated GPP decreased by an average of 20% in the Palm and Dwarf forests at high elevations as a result of the slow recovery of the canopy. Simulated annual NPP in the Palm and Dwarf forests also did not recover to pre-Hugo levels within 5 years. Simulated storages of SOC, CO₂ emission from decomposition of SOC and total soil nitrogen increased slightly but N mineralization rate increased significantly in all four vegetation types due to the massive input of plant materials from Hugo at low elevations and the slow decomposition at high elevations.     

宁夏东部风沙区固定沙丘土壤性质小尺度空间变异特征

随着流动沙丘的不断固定,不同地形部位的土壤机械组成、有机碳(SOC)和全氮(TN)含量的变化存在差异.为了探明固定沙丘的土壤机械组成与土壤养分空间变异特征,本研究以宁夏黄沙古渡固定沙丘为对象,分析了固定沙丘不同部位、不同土层的风沙土机械组成、SOC和TN特征.结果 表明:各土层土壤机械组成均以中沙和细沙为主.SOC和T...     

Modeling gross primary productivity of alpine meadow in the northern Tibet Plateau by using MODIS images and climate data

The Vegetation Photosynthesis Model (VPM) was used to simulate the gross primary productivities (GPP) of the alpine meadow ecosystem in the northern Tibet Plateau at three different spatial resolutions of 0.5 km, 1.5 km and 2.5 km, respectively. The linear relationships between enhanced vegetation indices (EVI) and GPP, with higher correlative coefficients, were better than those between normalized difference vegetation indices (NDVI) and GPP at the three resolutions. VPM could well simulate the seasonal changes and inter-annual variations of GPP, with similar trends at the three resolutions. There were significant differences (P < 0.0001) among the three modeled GPP with the three resolutions. Therefore, the modeled GPP at high resolution could not be directly extrapolated to low resolution, and vice versa. The contribution levels of different model parameters, including photosynthetically active radiation (PAR), air temperature (Ta), NDVI, EVI and land surface water indices (LSWI), to modeled GPP could vary with spatial resolution based on multiple stepwise linear regression analysis. This indicated that it was important to choose parameters properly and consider their effects on modeled GPP.     

甘肃白龙江流域净生态系统生产力时空变化

净生态系统生产力(NEP)是估算区域植被碳源、碳汇的重要指标。以甘肃白龙江流域为研究区,结合MODIS与气象数据对2000—2013年的流域净生态系统生产力时空变化进行了研究,并探讨了典型地形因子对NEP的影响。结果表明:(1)2000—2013年甘肃白龙江流域单位面积NEP平均为226.65 g C m~(-2)a~(-1),碳汇区主要分布在白龙江上游两岸、岷江西岸、白水江南岸、大团鱼河两岸的山地林区,碳源区主要分布在武都区、迭部县北缘的高寒草甸区等。(2)从不同植被类型上看,常绿阔叶林、常绿/落叶阔叶混交林单位面积NEP最高,高寒草地单位面积NEP最小,且耕地单位面积NEP增加最明显,常绿/落叶阔叶混交林单位面积NEP降低最明显。(3)2000—2013年研究区单位面积NEP总体上呈增加的趋势,增加明显的地区分布在流域的中部和西北部,4—9月为流域碳汇季节。(4)地形因子对甘肃白龙江流域NEP有明显影响,海拔4200 m以下多为碳汇区;陡坡区的碳汇能力的增长趋势低于缓坡区;阴坡的碳汇能力高于阳坡区。     

土地利用变化对三峡库区重庆段植被净初级生产力的影响

研究土地利用变化对区域植被净初级生产力(Net Primary Productivity,NPP)的影响对于明确区域植被固碳能力与土地利用变化的关系,以及维持生态系统结构稳定具有重要意义。以三峡库区重庆段为例,基于2000—2015年MOD17A3数据和土地利用数据,分析研究区NPP时空分布特征并从景观生态学的角度探讨土地利用变化对区域植被NPP的影响。研究表明:(1)NPP年均值16年间波动不大,空间分布上从东到西逐渐减少;(2)研究期内林地面积增加,耕地和草地面积减小,而NPP总量从25.6 TgC增加到了28.5 TgC,其中耕地NPP约占总量的44%,林地次之(40%),草地最少(14%),2000—2005年、2005—2010年、2010—2015年土地利用变化对NPP变化的贡献率分别为26.49%、59.76%、17.27%;(3)区域生态景观指数中的香农多样性指数SHDI、斑块密度PD与NPP呈正相关,而聚合度AI与NPP呈负相关,景观格局类型和景观格局变化均影响区域植被NPP的增长。要提高区域植被NPP,需优化土地利用格局,增加景观异质性和斑块密度,重视培育幼龄林,并控制成熟林的数量。     

白洋淀蝗区东亚飞蝗的分布与土壤的关系研究

从土壤的质地、含水量、pH值和含盐量等方面 ,研究了白洋淀东亚飞蝗Locustamigratoriamanilensis(Meyen)蝗区的土壤状况 ;并结合实地考察的样点处蝗虫密度情况 ,研究了蝗虫不同密度区土壤之间的差异。结果表明 ,研究区蝗虫密度在 3 0头 m2 以上的地区 ,土壤质地为粉砂壤土 ,土壤pH值为7 2 6～ 8 1 2 ,土壤含盐量为 0 0 67%～ 0 2 0 7%。粉砂壤土中 ,砂粒含量较多、粗粉粒含量较少的地方 ,是东亚飞蝗比较理想的生存与活动场所 ;土壤含水量偏高、呈弱碱性的地方 ,适于东亚飞蝗的生存和活动。在研究区 ,土壤含盐量差异对东亚飞蝗的密度分布没有明显的影响。     

Effectiveness of phytoremediation as a secondary treatment for polycyclic aromatic hydrocarbons (PAHs) in composted soil

A greenhouse study was conducted over a 12-month period to investigate the fate of polycyclic aromatic hydrocarbons (PAHs) in soil using phytoremediation as a secondary treatment. The soil was pretreated by composting for 12 weeks, then planted with tall fescue (Festuca arundinacea), annual ryegrass (Lolium multiflorum), and yellow sweet clover (Melilotus officinalis). Two sets of unvegetated controls also were evaluated, one fertilized and one unfertilized. Total PAH concentrations decreased in the tall fescue, annual ryegrass, and yellow sweet clover treatments by 23.9%, 15.3%, and 9.1%, respectively, whereas the control was reduced by less than 5%. The smaller two- and most of the three-ringed compounds--naphthalene, acenaphthylene, acenaphthene, fluorene, and anthracene--were not found in detectable concentrations in any of the treatments. The most probable number analysis for microbial PAH degraders did not show any statistically significant differences among treatments. There were significant differences among treatments (p < 0.05) for the residual concentrations of five of the target PAHs. Root surface area measurements indicated that tall fescue and annual ryegrass both had significantly higher root surface area than yellow sweet clover, although the two species were not significantly different from each other. The tall fescue treatment resulted in the highest root and shoot biomass, followed by annual ryegrass and yellow sweet clover, and also had the highest percent of contaminant removal after 12 months. These results imply a positive relationship between plant biomass development and PAH biodegradation.     

Quantification of central metabolic fluxes in the facultative methylotroph methylobacterium extorquens AM1 using 13C-label tracing and mass spectrometry

The metabolic fluxes of central carbon metabolism were measured in chemostat-grown cultures of Methylobacterium extorquens AM1 with methanol as the sole organic carbon and energy source and growth-limiting substrate. Label tracing experiments were carried out using 70% (13)C-methanol in the feed, and the steady-state mass isotopomer distributions of amino acids derived from total cell protein were measured by gas chromatography coupled to mass spectrometry. Fluxes were calculated from the isotopomer distribution data using an isotopomer balance model and evolutionary error minimization algorithm. The combination of labeled methanol with unlabeled CO(2), which enters central metabolism in two different reactions, provided the discriminatory power necessary to allow quantification of the unknown fluxes within a reasonably small confidence interval. In wild-type M. extorquens AM1, no measurable flux was detected through pyruvate dehydrogenase or malic enzyme, and very little flux through alpha-ketoglutarate dehydrogenase (1.4% of total carbon). In contrast, the alpha-ketoglutarate dehydrogenase flux was 25.5% of total carbon in the regulatory mutant strain phaR, while the pyruvate dehydrogenase and malic enzyme fluxes remained insignificant. The success of this technique with growth on C(1) compounds suggests that it can be applied to help characterize the effects of other regulatory mutations, and serve as a diagnostic tool in the metabolic engineering of methylotrophic bacteria.     

Environmental and Human Health Risk Assessment of Benzo(a)pyrene Levels in Agricultural Soils from the National Capital Region,Delhi, India

ABSTRACT

Exposure to benzo(a)pyrene (B(a)P) for health risk was studied in soils from the Delhi region, India. The mean and median concentrations of benzo(a)pyrene were 0.031 and 0.029 (±0.002) mg/kg, respectively. The lifetime average daily dose (LADD) for adults and children was 1.7 × 10^?8 mg kg^?1 d^?1 and 7.5 × 10^?8 mg kg^?1 d^?1, respectively, with incremental life time cancer risk (ILCR) of 1.2 × 10^?7 and 5.5 × 10^?7, respectively. The Index of Additive Cancer Risk (IACR) was 0.084. Our screening-level risk assessment shows that the observed ILCR and IACR values are much lower than the guideline values of 10^?6 ? 10^?4 (ILCR) and <1 (IACR), respectively, and therefore the measured B(a)P levels in soil may not portend environmental and human health risks.