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1.
Influence of larval rearing temperature on the quality of cold‐stored Oomyzus sokolowskii Kurdjumov (Hymenoptera: Eulophidae)
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Oomyzus sokolowskii, an important parasitoid of Plutella xylostella, has great potential for use in biological control. Storage at suboptimal temperature is valuable for increasing the shelf‐life of insect parasitoids. In this study, O. sokolowskii larvae were reared at 30/25, 25/25 and 25/20°C light/dark (65 ± 5% RH, 16 : 8 h L : D) until pupation. The pupae were then cold‐stored at 4 ± 1°C (60 ± 5% RH, full darkness). The pupae were removed out from the storage at 10, 20, 30 and 40 days after storage (DAS) and maintained at 25 ± 2°C until adults emerged or pupae died. Quality of the emerging adults and their F1 offspring were assessed. Incidence of parasitism by O. sokolowskii was higher at 30/25°C than at 25/20°C. Cold storage of O. sokolowskii pupae greatly affected the fitness of the parasitoid: adult emergence rates were lower in the 40 DAS treatment than in other treatments; when O. sokolowskii larvae developed at 25/25°C, female proportions of the emerged adults were lower in the 40 DAS treatment than in the 0 and 10 DAS treatments. Larval rearing temperature mildly affected the adult emergence rate, post‐storage developmental time and female proportion with a few exceptions. Number of parasitoids emerged per host pupa, and incidence of parasitism by the females were neither affected by larval rearing temperature nor cold storage duration. Trans‐generational effects on F1 offspring were evident in adult emergence rate, egg‐adult developmental time and female proportion which were negatively affected by long duration of storage (40 days), but not by larval rearing temperature with a few exceptions. In conclusion, O. sokolowskii pupae could be stored at 4°C for up to 30 days without significant fitness loss. 相似文献
2.
Maternal and progeny quality of Habrobracon hebetor Say (Hymenoptera: Braconidae) after cold storage
The ectoparasitoid Habrobracon hebetor Say (Hymenoptera: Braconidae) is an important biological control agent for lepidopterous pests of stored products. We investigated the effects of low temperature storage on the quality of adult parasitoids and their progeny. Newly emerged females were stored for 10, 20, 30, 40, 50, and 70 days at 5 ± 1 °C. Several reproductive and developmental parameters were then assessed to determine the quality of the adult parasitoids and their progeny. After more than 30 days of storage, there was a decrease in parental parasitism, but low temperature storage of parents had no effect on parasitism of the F1 generation. Parental longevity and fecundity decreased after more than 20 days of storage, but there was no effect of storage duration on the fecundity and longevity of the F1 generation until a storage duration of 50 days. Development time varied with storage duration but differences were within 2 days. Storage duration had no effect on the sex ratio of F1 and F2 generations. Our data show that H. hebetor can be cold stored for up to 20 days without adversely affecting the performance of the parasitoid. Therefore, short-term storage of H.hebetor adults could be used for maintaining and accumulating large numbers of parasitoids in mass rearing programs. 相似文献
3.
J. Tormos F. Beitia M. Alonso J.D. Asís S.F. Gayubo 《The Annals of applied biology》2010,156(2):179-185
In this work, we study the suitability of using dead medfly Ceratitis capitata pupae, killed by heat- or cold-shock, for the mass rearing of Spalangia cameroni, a pupal parasitoid of key pests. 100% mortality of medfly pupae could be accomplished with cold-shock at –20°C for 60 min or with heat-shock at 55°C for 30 min. Neither parasitism percentage nor sex ratio of the offspring differed significantly among heat-shocked, cold-shocked and untreated pupae. In addition, there was no significant difference in the percentage of parasitoids that aborted (♂♂ or ♀♀) among pupal treatments. Some of the pupae were covered with peat because the third larval instar of the medfly buries itself before pupation. However, the buried pupae were not parasitised at a greater or lesser rate than those not covered with peat. The percentage of parasitism was also unaffected by whether the pupae had been killed recently or had been stored at between 4°C and 6°C over 15 or 30 days. The use of dead hosts and later storage permitted the following: (a) the use of hosts over long periods of time; (b) a rapid increase in parasitoid numbers and (c) the availability of pupae killed at the most suitable postpupation times for the production of parasitoids. Furthermore, in biological control projects, the use of dead parasitised pupae in the field avoids the risk of enhancing the pest and allows an increase in parasitism in the field through the use of pupae treated with cold- or heat-shock. 相似文献
4.
The ectoparasitoid Habrobracon hebetor (Say) (Hymenoptera: Braconidae) is an important potential biological control agent for lepidopterous pests of stored products. We investigated the effects of long-term cold storage of diapausing and nondiapausing H. hebetor on their performance after cold storage. Mortality during storage increased with increasing storage duration, and the mortality of diapausing females was lower than that of nondiapausing females after 8, 12, and 16 weeks of storage. Longevity, egg laying, number of progeny produced, and time to 50% egg laying were all reduced, as compared with the culture females when parasitoids were reared at conditions that do not induce diapause. But, for females reared at 20 °C at conditions that induce diapause, all of these quality parameters did not differ from those of culture insects when the storage duration was 8 weeks or less. The percentage of female F1 offspring was always lower for cold stored insects than for the culture insects. Presence of a male after cold storage did not impact any of the quality parameters measured. Thus, rearing parasitoids at 20 °C and 10L:14D and then storing them for up to 8 weeks at 5 °C would produce parasitoids that are similar to culture parasitoids, except that the percentage of females is lower than that in the cultures (36% vs. 52%). 相似文献
5.
A P Queiroz A F Bueno A POMARI-FERNANDES M L M Grande O C Bortolotto D M Silva 《Neotropical Entomology》2017,46(2):182-192
We conducted three bioassays to evaluate the effect of low-temperature storage of eggs (host) and pupae and adults (parasitoid) on the biology and parasitism capacity of the egg parasitoid Telenomus remus (Nixon) (Hymenoptera: Platygastridae). Viable stored Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) eggs were parasitized to the same degree or even higher than fresh eggs when stored until 14 days at 5°C or until 21 days at 10°C. In contrast, the percentage of parasitized sterilized eggs was equal to the control only when stored for 7 and 14 days. Survival of T. remus pupae declined with storage time at both studied temperatures (5 and 10°C). However, after 7 days of storage, survival of pupae was still 86.3 and 64.9% at 10 and 5°C, respectively. The number of adult male survivors remained similar until the fourth storage day at both 5 and 10°C. In contrast, female survival did not differ until day 8 at 10°C or day 6 at 5°C. Parasitism capacity of stored adults was not altered by storage compared with the control. Therefore, we conclude that the maximal storage time at 10°C is 21 days for viable C. cephalonica eggs and 7 days for T. remus pupae, while parasitoid adults should not be stored for more than 4 days at either 5 or 10°C. 相似文献
6.
N. Özder 《Archives Of Phytopathology And Plant Protection》2013,46(4):296-299
Abstract Trichogramma cacoeciae Marchall, T. brassicae Bezdenko and T. evanescens Westwood could be useful in biological control programmes of agricultural insect pest. The possibility of storing adult Trighogramma species at low temperatures, and the effect of such storage on the quality of the parasitoids and its fecundity were studied. Trighogramma cacoeciae, T. brassicae and T. evanescens adults were stored for 1, 2, 3, 4 and 5 days at 4 ± 1°C in a refrigerator, 60 – 70%RH and full darkness. Storage at 4 ± 1°C reduced fecundity and longevity of female parasitoids. 相似文献
7.
Hypoxia periods of 6, 12, 24, 48, and 72?h combined with temperatures of 15°C, 20°C and 25°C were applied to Diachasmimorpha longicaudata pupae in packaging conditions. Alternating temperatures of 26–20–26°C were applied to pupae in three sequential 4-h periods less than 12?h of hypoxia, and the effect of hypoxia on the pupae was evaluated under ambient conditions in commercial shipping. The emergence, longevity, fecundity and flight ability of adult parasitoids were recorded. The duration of hypoxia (>24?h) and the higher temperature (25°C) significantly reduced the emergence and longevity of adults. The different temperatures, including the variation in temperature combined with hypoxia, showed no significant effect on fecundity or flight ability. The temperature and humidity recorded during commercial shipping under hypoxia did not show any effect on the emergence of parasitoids. As a consequence, hypoxia was only notably significant after 24?h, which occasionally occurs in practical packaging processes. The use of hypoxia for shipping D. longicaudata pupae can be widely recommended, but long shipping periods and high temperatures should still be avoided. 相似文献
8.
The sublethal effects of lufenuron and lufenuron?+?fenoxycarb were tested on the adult stage of the parasitoid wasp Habrobracon hebetor under laboratory conditions. Bioassay tests were carried out in the incubator at a temperature of 26?±?1?°C, relative humidity of 65?±?10% and photoperiod of 16:8 (L:D). Exposure cages were used for the experiments. Tests were conducted in three replications. Young female wasps were exposed to the residues of the pesticides’ sublethal concentrations (LC25) after 1, 24, 48 and 72 h, and then their mortalities were recorded. Results showed that both insecticide treatments had significant differences compared to the control, but not between themselves (df?=?1, F?=?29.21, α?<?.0001). It was also revealed that the time between insecticides application and H. hebetor release would significantly affect the survival of searching parasitoids (df?=?3, F?=?237.01, α?<?.0001). In this regard, the releasing programme should be postponed for at least 24 h after using these insect growth regulators. 相似文献
9.
Nihal Özder 《Biocontrol Science and Technology》2004,14(5):441-447
The parasitism rates by Trichogramma cacoeciae Marchal (Hymenoptera, Trichogrammatidae) using Ephestia kuehniella Zell. (Lepidoptera, Pyralidae) eggs held at 0, 4 and 8°C and for up to 31 days was measured. Parasitism was lowest on eggs held at 8°C and highest on eggs held at 0°C. The highest parasitism, 97.8%, was measured for parasitoids attacking eggs held for 3 days and stored at 0°C. Parasitism of eggs stored at all three temperatures decreased with increasing duration of storage. The number of T. cacoeciae successfully developing and emerging as adults after storage in E. kuehniella eggs held at 0, 4 and 8°C was measured. Parasitoid emergence was >83% from E. kuehniella eggs stored at 8°C for 3 weeks. Storage at 0°C caused a significant decline in parasitoid emergence after 2 weeks (P<0.05). Storage at 0°C for more than 4 weeks reduced fecundity by 50%. T. cacoeciae parasitized the highest number of E. kuehniella eggs 1 day after adult emergence. The oviposition period lasted 6–7 days, although the parasitoids lived up to 13–14 days. Impact of storage time and temperature on parasitism rates by T. cacoeciae stored while in E. kuehniella eggs was measured. As storage time and temperature increased, subsequent parasitism rates of resulting adult T. cacoeciae decreased. Eggs of E. kuehniella can be stored at 0°C for up to 31 days. Trichogramma cacoeciae developing in eggs of E. kuehniella can be stored at 4°C for up to 5 weeks prior to release. 相似文献
10.
Before parasitoids and predators are fully endorsed as biological control agents in storage facilities, a reliable technique must be developed to determine how much they contribute to the overall insect contamination of stored commodities. Because determining the origin of insect fragments by visual examination is difficult, labor-intensive, and requires special skills, we propose using immunological techniques to differentiate among insect species biochemically. In the example presented here, we generated monoclonal antibodies (MAbs) against the parasitic wasps Laelius pedatus (Say) and Bracon hebetor Say. The MAbs reacted with all life stages and both sexes of the parasitoids. In Western blots of acrylamide and agarose gels, the MAbs recognized high molecular weight proteins (>500 kDa) composed of multiple subunits, with mildly acidic to neutral isoelectric focusing points. The MAbs did not cross-react with the additional 22 insect species we tested in an indirect enzyme-linked immunosorbent assay. These data suggest that MAb-based immunoassays could be used to differentiate among beneficial and destructive insects found in stored products. 相似文献
11.
Under stressful thermal environments, insects adjust their behavior and physiology to maintain key life‐history activities and improve survival. For interacting species, mutual or antagonistic, thermal stress may affect the participants in differing ways, which may then affect the outcome of the ecological relationship. In agroecosystems, this may be the fate of relationships between insect pests and their antagonistic parasitoids under acute and chronic thermal variability. Against this background, we investigated the thermal tolerance of different developmental stages of Chilo partellus Swinhoe (Lepidoptera: Crambidae) and its larval parasitoid, Cotesia sesamiae Cameron (Hymenoptera: Braconidae) using both dynamic and static protocols. When exposed for 2 h to a static temperature, lower lethal temperatures ranged from ?9 to 6 °C, ?14 to ?2 °C, and ?1 to 4 °C while upper lethal temperatures ranged from 37 to 48 °C, 41 to 49 °C, and 36 to 39 °C for C. partellus eggs, larvae, and C. sesamiae adults, respectively. Faster heating rates improved critical thermal maxima (CTmax) in C. partellus larvae and adult C. partellus and C. sesamiae. Lower cooling rates improved critical thermal minima (CTmin) in C. partellus and C. sesamiae adults while compromising CTmin in C. partellus larvae. The mean supercooling points (SCPs) for C. partellus larvae, pupae, and adults were ?11.82 ± 1.78, ?10.43 ± 1.73 and ?15.75 ± 2.47, respectively. Heat knock‐down time (HKDT) and chill‐coma recovery time (CCRT) varied significantly between C. partellus larvae and adults. Larvae had higher HKDT than adults, while the latter recovered significantly faster following chill‐coma. Current results suggest developmental stage differences in C. partellus thermal tolerance (with respect to lethal temperatures and critical thermal limits) and a compromised temperature tolerance of parasitoid C. sesamiae relative to its host, suggesting potential asynchrony between host–parasitoid population phenology and consequently biocontrol efficacy under global change. These results have broad implications to biological pest management insect–natural enemy interactions under rapidly changing thermal environments. 相似文献
12.
Umberto Bernardo Luigi Iodice Raffaele Sasso Paolo A. Pedata 《Biocontrol Science and Technology》2008,18(9):921-933
Thripobius javae (Girault) was introduced in 1995 from Israel into Italy to control the greenhouse thrips, Heliothrips haemorrhoidalis (Bouché). Following introduction, successive augmentative releases of this parasitoid gave unsatisfactory and contradictory results, mainly due to the difficulty in synchronising its availability in sufficient number at the time of release. Efficient storage of this biological control agent could improve its current production and use. The effects of different sets of storage techniques at a single temperature and with a combination of different temperatures and instars on several fitness traits (residual developmental time to adult emergence after the end of storage, pupal mortality, longevity with and without hosts and progeny of emerged adults) were evaluated in order to determine the best conditions for storing the parasitoid.
For the pupal stage, increasing storage up to 14 days, at 10°C, gave only a moderate reduction (33%) of a modified composite quality index of its fitness. In contrast, when adults were stored for more than 10 days, at 15°C, residual longevity and progeny were reduced significantly. A combination of two temperatures (10 and 15°C) for pupal storage and a combination of pupal (10°C) and adult (15°C) storage had detrimental effects on parasitoid fitness. Temperatures of storage lower than 15 and 10°C had detrimental effects on adults and pupae, respectively. 相似文献
13.
Heat shock proteins (HSPs) are known as chaperones that help with folding of other proteins when cells are under environmental stresses. The upregulation of HSPs is essential for cold survival during insect diapause. The ectoparasitoidHabrobracon hebetor, a potential biological control agent, can enter reproductive diapause when reared at low temperature and short photoperiod. However, the expression of HSPs during diapause of H. hebetor has not been studied. In this study, we sequenced and characterized the full-length complementary DNAs of three Hsp70 genes (HhHsp70I, HhHsp70II and HhHsp70IIl) from 11. hebetor. Their deduced amino acid sequences showed more than 80% identities to their counterparts from other insect species. However, the multiple se- quence alignment among the three deduced amino acid sequences of HhHsp70s showed only 46% identities. A phylogenetic analysis of the three HhHsp70s and all other known Hsp70 sequences from Hymenoptera clustered all the Hsp70s into four groups, and the three HhHsp70s were distributed into three different groups. Real-time quantitative poly- merase chain reaction analysis showed that the expression of the three HhHspTO genes in H. hebetor reared at different conditions was quite different. HhHspTOI showed higher relative expression when H. hebetor were reared at 27.5℃ than at two lower temperatures (17.5℃ and 20℃) regardless of the photoperiod, whereas HhHspTOII showed higher ex- pression when H. hebetor were reared at 20℃ and 10 : 14 L : D than when reared at 17.5℃ and either 16 : 8 L : D or 10 : 14 L : D. In contrast, HhHSP7OIIIwas expressed at similar levels regardless of the rearing conditions. These results may suggest functional differences among the three HhHspTO genes in H. hebetor. 相似文献
14.
Ibrahim Boukary Baoua Laouali Amadou Adama Kabore Clementine L. Dabire-Binso 《Biocontrol Science and Technology》2018,28(4):404-415
Pearl millet is one of the major staple food crops in Sub-Sahelian Africa, and the millet head miner (MHM) [Heliocheilus albipunctella] is its major pest, causing serious economic damage in the maturity period. We studied the dispersion patterns of the endogenous ectoparasitoid, Habrobracon hebetor (Hymenoptera: Braconidae), after augmentative releases in pearl millet fields for biological control of the MHM, in 2010 and 2011 in Burkina Faso and Niger. The parasitoids were released using 15 jute bags per release site. Parasitoid dispersion was indirectly monitored through weekly assessments of MHM parasitism by H. hebetor at different distances from release points (0, 3 and 5?km) and in control villages (15?km). Our findings indicate that the jute bags released approximately 900–1000 parasitoids per site over a period of three weeks. This initial parasitoid population led to higher parasitism of MHM larvae at the site of dissemination compared to farms at distances of 3 and 5?km. However, usually after five weeks, successive generations of H. hebetor dispersed up to 3?km, causing high levels of MHM larval mortality, which sometimes is similar to those of the release points. Based on these results, we recommend the release of parasitoids at sites spaced 3?km for timely and more efficient control of MHM populations. 相似文献
15.
The multicolored Asian ladybird beetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), is considered an important generalist predator that can be used as a biological control
agent against Hemiptera Sternorrhyncha, Thysanoptera, and the eggs and larvae of Lepidoptera, Coleoptera and Diptera. There
are currently abundant natural resources of overwintering H. axyridis in Asia and North America. Given its potential as a biological control agent, methods can be developed to increase its effectiveness
for pest control. The availability of an adequate cold storage method would enable the use of field-collected pre-wintering
ladybirds for pest suppression in the following season. We studied the effect of cold storage (30, 60, 90, 120 and 150 days
stored at −3, 0, 3 and 6°C) on survival, fecundity and predation in field-collected populations. The survival of both female
and male ladybirds decreased significantly as storage duration increased at −3°C and 0°C. The ladybirds showed more than 80%
survival when they were stored for 150 days at 3°C and 6°C. Long-term cold storage had different effects on the fecundity
of H. axyridis at different temperatures. Prolonged cold storage at both 3°C and 6°C shortened pre-oviposition duration and had no adverse
effect on reproductive capacity as compared to that of unstored individuals. The adults that experienced 90-day storage at
0°C had the shortest pre-oviposition duration and the largest reproductive capacity. The individuals that were stored for
150 days at 3°C consumed significantly more aphids than the unstored ones. Generally, 3–6°C is a suitable temperature for
cold storage of the ladybird without any reduction in fitness. This study will help the exploitation and application of pre-wintering
H. axyridis for the biological control of insect pests. 相似文献
16.
Cold tolerance studies were conducted under laboratory conditions for coccons ofAllorhogas pyralophagus Marsh, a Mexican parasitoid of graminaceous borers. By storage, developmental time (from cocoon to adult emergence) could
be extended by 2 to 6 times. However, cocoons stored for more than 14 days at 2°C failed to survive, while at 5 and 10°C,
about 50% emergence was recorded for upto 21 days of storage. With respect to survival and adult longevity, 10°C seemed to
be the most suitable storage temperature. Pre-emergence period was also significantly increased by storing cocoons for 21
to 35 days at this temperature. Sex-ratio of emerging adults was not significantly affected by storage. Fecundity was adversely
affected in all the treatments except in the case of females emerging from cocoons stored at 5°C for 7 days. The progeny of
parasitoids which emerged from cocoons stored at 5 and 10°C for 35 days consisted of only males. It is clear from the present
study thatA. pyralophagus cocoons are more amenable to short-term storage.
Contribution No. 285/87 of the Indian Institute of Horticultural Research, Bangalore-560 089. 相似文献
17.
The survival of a braconid parasitoid Habrobracon hebetor was investigated on nucleopolyhedrovirus (NPV)-infected Spodoptera exigua larvae. The second-instar larvae were exposed to 30, 51.4 and 180 PIB/mm2 of Mamestra brassicae NPV (MbMNPV) as under-LD50, LD50 and over-LD50 values, respectively. They were accessible to be parasitized by H. hebetor after 24, 48 and 72 h post-treatment. Infection of the larvae with MbNPV was deleterious to the survival and parasitism of H. hebetor. The survival of H. hebetor in MbNPV-infected S. exigua larvae was dependent on the interval between viral infection and parasitization, as well as on the treatment dose of MbMNPV; very few adults of parasitoid emerged from infected hosts when host larvae were exposed to 180 PIB/mm2 of MbNPV on 72-h interval treatment. The inoculation dose of MbNPV and the timing of parasitoid release had significant effect on the development of H. hebetor on virus-infected hosts. Field applications of virus for biocontrol of S. exigua may lead to substantial mortality of immature parasitoids. 相似文献
18.
Hossein Mahi Arash Rasekh J.P. Michaud Parviz Shishehbor 《Entomologia Experimentalis et Applicata》2014,153(1):10-19
Cold storage is one means of preserving parasitoids prior to release in augmentation biological control programs. This study examined the feasibility of storing larval and pupal stages of a sexual population of Lysiphlebus fabarum Marshall (Hymenoptera: Braconidae: Aphidiinae) at 6 ± 1 and 8 ± 1 °C, 50–60% r.h., and L14:D10 photoperiod. These life stages were stored for periods of 1, 2, and 3 weeks under fluctuating thermal regimes (2 h daily at 21 ± 1 °C). Generally, pupae gave better results than larvae, and 6 °C was better than 8 °C, considering wasp survival, wasp size (tibial and antennal lengths), egg load, and egg size. The best results were obtained with pupae stored for 2 weeks under a fluctuating temperature regime at 6 °C. Females emerging from this treatment did not differ from controls (developing directly at 21 °C) in body size, egg size, or progeny sex ratio, and suffered less than 20% mortality. Egg loads were reduced in these wasps, but the reductions were substantially less than occurred in other 2‐week‐storage treatments. Wasps stored in this manner successfully parasitized similar numbers of aphids as controls and produced similar progeny sex ratios. These results reveal a suitable set of low‐temperature conditions that can be used to delay the development of L. fabarum for 2 weeks with minimal impact on wasp fitness. 相似文献
19.
Cold stored ectoparasitoid of Cydia fruit moths released under different temperature regimes 总被引:1,自引:0,他引:1
Cold storage of parasitoids to be used in biological control programs is desirable but risky for the performance of the stored
parental generation as well as for its offspring. We studied the performance of cold stored and unstored parasitoids after
release at different temperature regimes in the laboratory at the level of two subsequent generations in Hyssopus pallidus (Askew) (Hymenoptera: Eulophidae). This gregarious ectoparasitoid is a candidate biocontrol agent of Cydia pomonella L. (Lepidoptera: Tortricidae) and Cydia molesta (Busck) (Lepidoptera: Tortricidae) larvae, two fruit pests of high economic significance in apple cultivation. Cold storage
for 14 days at 4°C imposed to the pupal stage of the parasitoid did not reduce the parasitism capacity of the parental generation,
nor did it alter the female biased sex ratio of the progeny. Remarkably, this short-term storage of the parental generation
exhibited a significant and consistently positive effect on offspring weight throughout all ambient temperature regimes, resulting
in an increased offspring weight. Furthermore, offspring number was only reduced after release at low ambient temperatures,
but not at 25°C and 30°C. Irrespective of whether the parasitoids originated from the stored or unstored group, highest parasitism
rate was achieved at temperatures above 20°C. In conclusion, this candidate biocontrol agent can be cold stored for short
periods without any measurable quality loss after release at most except at low ambient temperatures. Our findings suggest
that H. pallidus is a thermophilous parasitoid that will perform best when applied at warm ambient temperatures in fruit orchards. 相似文献
20.
Frank H. Arthur 《Insect Science》2008,15(2):133-140
A series of field trials were conducted in a commercial food storage facility to evaluate exposure of stored‐product insects to aerosol formulations of synergized pyrethrins and the insect growth regulator methoprene. When adults of Tribolium castaneum (Herbst), the red flour beetle, and Tribolium confusum (Jacqueline DuVal), the confused flour beetle were exposed with and without a food source to synergized pyrethrin aerosol, there was no difference in adult mortality with respect to availability of food at either 7 or 14 days after exposure (P≥ 0.05). However, mortality was lower in T. confusum (40.4% and 79.3% with flour at 7 and 14 days, 38.9% and 84.8% without flour at 7 and 14 days) compared to T. castaneum (96.5% and 99.8% with flour at 7 and 14 days, 91.0% and 98.7% without flour at 7 and 14 days). Few late‐stage larvae and pupae of either species exposed to the pyrethrin aerosol emerged as adults. In tests with methoprene aerosol, adult emergence of exposed 3‐and 4‐week‐old larvae of T. confusum was less than 2%. Only 0.3% of 4‐week‐old larvae of T. castaneum exposed in open and obstructed areas emerged as adults. Emergence of adults from eggs of Plodia interpunctella (Hübner), the Indianmeal moth, embedded in culture media and exposed to the methoprene aerosol was 13.2%± 3.5%. Results show that the aerosols evaluated in our study could give effective control of some of the major stored‐product insect pests in commercial food storage facilities, and may offer an alternative to fumigation. 相似文献