氟铃脲对斜纹夜蛾酚氧化酶活性的影响

【目的】探讨氟铃脲对斜纹夜蛾Spodoptera litura酚氧化酶活性的影响.【方法】应用酶动力学法,在确定斜纹夜蛾酚氧化酶最适反应条件基础上,测定氟铃脲与酚氧化酶直接作用及氟铃脲处理幼虫后酚氧化酶的活性.【结果】以邻苯二酚为底物,斜纹夜蛾酚氧化酶的最适pH 6.5,最适温度30℃.氟铃脲没有和酚氧化酶结构中的铜离子结合,而是直接与酶蛋白发生作用.氟铃脲浓度低于1 153 mg/L对离体酚氧化酶有激活作用,在138 mg/L激活作用最大,浓度高于1 153 mg/L则产生抑制作用.用46 mg/L和92 mg/L亚致死剂量氟铃脲连续处理5龄幼虫不同时间后,酚氧化酶活性均显著提高,且高浓度的激活作用大于低浓度的激活作用.进一步测定药剂处理幼虫24,48和72 h的血淋巴、表皮和头部酚氧化酶活性,随着氟铃脲处理时间延长,两个剂量的氟铃脲对血淋巴、表皮及头部酚氧化酶的激活作用逐渐增大,氟铃脲对血淋巴酚氧化酶的激活作用最大,表皮次之,头部最低.氟铃脲处理幼虫后,其预蛹和蛹酚氧化酶的活性也显著提高,表现出一定的后效应.【结论】一定浓度范围内,氟铃脲对斜纹夜蛾酚氧化酶具有激活作用.     

杀菌剂丙环唑对斜纹夜蛾的毒性

在研究比较了11种农药对斜纹夜蛾Spodoptera litura细胞（简称SL细胞）的毒杀活性基础上,选择毒杀活性最高的杀菌剂丙环唑,对其毒理学机理进行进一步研究。结果表明,丙环唑的细胞毒力最高,在100 μg/mL浓度下处理后48 h,SL细胞的死亡率为98.08％。处理后36 h,丙环唑对SL细胞的LC₅₀值为20.31 μg/mL。丙环唑能明显降低SL细胞的蛋白质含量。以0.5 μg/头的丙环唑注射斜纹夜蛾4龄幼虫,处理后72 h,试虫血淋巴总含量及血细胞数分别下降了26.80％和25.26％;在1.0 μg/头的剂量下,则分别下降了37.67%和36.32%。以0.5 μg/头和1.0 μg/头的丙环唑注射处理后,斜纹夜蛾幼虫体重显著降低。此外,丙环唑能降低斜纹夜蛾幼虫血淋巴含糖量及血淋巴蛋白质含量。在注射处理后96 h和120 h,丙环唑对斜纹夜蛾4龄幼虫的LD₅₀值分别为0.59 μg/头和0.45 μg/头。丙环唑对SL细胞和斜纹夜蛾幼虫均具有较好的毒杀活性,显示出丙环唑类似物控制害虫的可能性。     

Laboratory evaluation of flight activity of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae)

Abstract  The flight activity of Spodoptera litura in tethered conditions is evaluated using a computer-mediated flight-mill in the laboratory. The 3–4-day-old moths fly longer and farther than those of other ages. Male and female moths exhibit similar flight activity. Mating status does not influence the flight duration and distance of 2-day-old females. However, these two flight parameters with a 6-day-old mated female is significantly lower than that of unmated ones. The optimum temperature for flight ranged from 16–24°C, whereas the optimum RH ranged from 60%–100%. During 72-h period, the total flight duration and distance of 1-day-old male and female moths were 19.6 h (± 5.8) and 83.3 km (± 28.4), and 24.0 h (± 7.0) and 105.4 km (± 37.4), respectively. These results indicate that S. litura has a great potential to undertake long-distance migratory flights.     

斜纹夜蛾对氯氟氰菊酯不同抗性水平与解毒代谢酶的关系

为探讨斜纹夜蛾Spodoptera litura (Fabricius)对氯氟氰菊酯抗性水平与解毒代谢酶之间的关系, 以泰安郊区对氯氟氰菊酯抗性为543.7倍的斜纹夜蛾田间种群为材料, 研究了药剂汰选与否的抗性动态及不同抗性水平的解毒代谢酶活性变化。结果表明: 室内继代饲养至第30代, 不接触任何药剂的抗性下降至102.3倍, 用氯氟氰菊酯汰选28代后, 抗性上升到3 049.3倍, 而在药剂汰选至第14代, 抗性已至2 593.8倍时, 停止用氯氟氰菊酯汰选, 到第30代的抗性又降至786.3倍。表明斜纹夜蛾抗氯氟氰菊酯田间种群, 在无药剂选择压力时抗性水平会显著下降, 继续给予药剂汰选会使抗性水平显著上升。检测斜纹夜蛾田间种群5龄幼虫中肠酯酶和谷胱甘肽S-转移酶活性, 发现与敏感种群有显著性差异, 而多功能氧化酶O-脱甲基活性与敏感种群的差异不明显; 给予氯氟氰菊酯药剂汰选, 酯酶、谷胱甘肽S 转移酶和多功能氧化酶O-脱甲基3种酶的活性均呈显著增加趋势; 停止用氯氟氰菊酯汰选后, 3种酶的活性又呈显著下降趋势; 不接触任何药剂, 随着饲养世代数的增加, 其酯酶和谷胱甘肽S-转移酶的活性也呈下降趋势。结果提示斜纹夜蛾幼虫酯酶、谷胱甘肽S-转移酶和多功能氧化酶O-脱甲基活性的提高是斜纹夜蛾对氯氟氰菊酯抗性上升的重要原因。     

黄皮种子提取物对斜纹夜蛾幼虫的杀虫活性及有效成分鉴定

【目的】测定黄皮Clausena lansium种子提取物对斜纹夜蛾Spodoptera litura幼虫的拒食活性,对有效成分进行分离、鉴定,并进一步测定其拒食活性、生长发育抑制活性及细胞毒性,为筛选斜纹夜蛾生态防治植物源杀虫剂提供依据。【方法】采用叶蝶法测定黄皮种子甲醇提取物及其石油醚、乙酸乙酯、正丁醇和水4种溶剂萃取物对斜纹夜蛾3龄幼虫的非选择性拒食活性和生长发育抑制活性;用硅胶柱层析进行石油醚萃取物和乙酸乙酯萃取物拒食活性成分分离;用核磁共振谱(NMR)进行拒食有效成分结构鉴定;用MTT法测定拒食活性成分对SL细胞毒性。【结果】黄皮种子甲醇提取物对斜纹夜蛾3龄幼虫具有较强的拒食活性,活性成分主要分布于石油醚相和乙酸乙酯相中;石油醚相和乙酸乙酯相经硅胶柱层析分离得10个馏分,以黄色晶体馏分2的拒食活性最强;经分离纯化及核磁共振氢谱和碳谱鉴定,黄皮新肉桂酰胺B(lansiumamide B)为主要的拒食活性成分,对斜纹夜蛾3龄幼虫48 h拒食中浓度为214.95μg/m L,同时具有较强的细胞毒性,对SL细胞48 h抑制中浓度为10.09μg/m L。【结论】黄皮种子提取物对斜纹夜蛾幼虫具有较强的拒食活性及细胞毒性,黄皮新肉桂酰胺B是主要活性成分之一,作为植物源杀虫剂开发具有较好的发展前景。     

番荔枝内酯化合物布拉它辛对斜纹夜蛾的杀虫活性及对SL细胞的致凋亡作用

为了明确番荔枝内酯化合物布拉它辛的杀虫活性和探索杀虫作用机理,本研究采用浸叶法测定该化合物对斜纹夜蛾Spodoptera litura幼虫的生物活性,采用MTT检测法和流式细胞术,研究该化合物对斜纹夜蛾离体培养卵巢细胞（SL细胞）的细胞毒力和致细胞凋亡作用,以及对SL细胞线粒体膜电位的影响。结果表明: 布拉它辛不仅对斜纹夜蛾幼虫具有良好的拒食活性,处理后24 h,对2龄和3龄幼虫的AFC₅₀值分别为60.25 μg/mL和86.73 μg/mL,还对幼虫生长有良好的抑制作用。布拉它辛处理SL细胞后24 h和48 h,IC50值分别为22.32 μg/mL和10.03 μg/mL。流式细胞仪检测结果表明,布拉它辛对SL细胞具有明显的致凋亡作用,可导致SL细胞线粒体膜电位显著下降。本研究表明布拉它辛对斜纹夜蛾幼虫具有良好的拒食活性与生长抑制作用, 并且该化合物能明显抑制SL细胞的增殖,诱导细胞凋亡,降低线粒体膜电位。因此,布拉他辛具有广阔的研究应用前景。     

Bioefficacy of Ceasalpinea bonduc (L.) Roxb. against Spodoptera litura Fab. (Lepidoptera: Noctuidae)

Bioefficacy of hexane, chloroform and ethyl acetate extracts of the seeds of Ceasalpinea bonduc (L.) Roxb. was studied against third instar larvae of Spodoptera litura at 0.25, 0.5, 1, 2.5 and 5.0% concentrations. Significant antifeedant, larvicidal and pupicidal activities and least LC₅₀ values were observed in chloroform extract. The chloroform extract was subjected to fractionation using silica gel column chromatography. Six fractions were obtained; among these, the third fraction showed high antifeedant, larvicidal and pupicidal activities at 1000 ppm concentration. Abnormalities in adults were also observed. All the activities were concentration dependent. C. bonduc could be useful in integrated pest management programme.     

Effect of plant secondary metabolites on common cutworm,Spodoptera litura (Lepidoptera: Noctuidae)

The effect of various flavonoids, lectins and phenyl β‐_D‐glucoside on larval survival, weights and the activities of digestive (total serine protease and trypsin) and detoxifying (esterase and glutathione‐S‐transferase) enzymes of Spodoptera litura larvae at 7 days after treatment was studied through diet incorporation assay. Flavonoids (rutin, chlorogenic acid, quinic acid, caffeic acid, naringenin, quercitin, kaempferol, myricetin, catechin, and ferulic acid) were incorporated in artificial diet at 100, 500 and 1000 ppm, lectins: groundnut leaf lectin (GLL), concavalin A (ConA) and phenyl β‐_D‐glucoside at 1, 2 and 5 μg/mL. Flavonoids such as rutin, quercitin and kaempferol at 1000 ppm were more toxic to S. litura larvae than quinic acid, caffeic acid, naringenin, myricetin, catechin, and ferulic acid. Larval growth and development were significantly reduced in S. litura larvae fed on a diet with GLL and ConA at 5 μg/mL compared to the larvae fed at 2 and 1 μg/mL concentrations. The larvae fed on flavonoid‐treated diets showed significant reduction in serine protease, trypsin and esterase activities. The flavonoids such as rutin, chlorogenic acid, quinic acid, naringenin, quercitin, kaempferol and myricetin, and lectins, GLL and ConA can be utilized in insect control programs.     

Sub-lethal effects of SpltMNPV infection on developmental stages of Spodoptera litura (Lepidoptera: Noctuidae)

Application of different doses of SpltMNPV on final instars of Spodoptera litura showed dose-related mortality. A significant increase in the time for pupation and progeny mortality as well as reduced fecundity was observed in surviving larvae. Impact of sublethal doses on subsequent generation needs attention in designing baculovirus-based pest management.     

不同温度下斜纹夜蛾的两性生命表

【目的】斜纹夜蛾Spodoptera litura(Fabricius)是一种鳞翅目夜蛾科食叶害虫,在不同的寄主、饲料等条件下表现出不同的生长发育特点。本实验旨在探究温度对斜纹夜蛾生长发育状况和种群参数的影响。【方法】在室内人工气候箱20±1℃,27±1℃和30±1℃(相对湿度和光周期分别为65%±10%和14L∶10D)条件下饲养斜纹夜蛾,观察和组建斜纹夜蛾在3种温度下的年龄-阶段两性生命表。【结果】斜纹夜蛾各个阶段的发育历期随温度升高变短,成虫产卵前期(adult preoviposition period,APOP)和总产卵前期(total preoviposition period,TPOP)同样表现为随温度升高而变短。此外,20±1℃处理的平均产卵量最高(1 810.36粒/雌),27±1℃处理次之(1 623.51粒/雌),30±1℃处理最低(1 084.60粒/雌)。斜纹夜蛾在不同温度条件下内禀增长率(r)、周限增长率(λ)和净增殖率(R0)有显著差异,20±1℃处理的r和λ显著低于27±1℃处理和30±1℃处理;27±1℃处理的R0显著大于20±1℃和30±1℃处理;20±1℃,27±1℃和30±1℃下平均世代周期(T)分别为52.51,30.63和26.87 d,依次减少且相互间差异显著(P0.05)。【结论】斜纹夜蛾在27±1℃和30±1℃温度条件下均表现出很强的增殖能力,该结果可为田间综合防治斜纹夜蛾提供理论依据。     

斑点野生稻拒食活性组分的分离及其对斜纹夜蛾消化酶活性的影响

为了进一步评估斑点野生稻Oryza punctata的抗虫性,采用液 液分配萃取和硅胶柱层析的方法,从斑点野生稻甲醇提取物中分离获得石油醚、氯仿、乙酸乙酯、正丁醇和水的萃取物,测定了其对斜纹夜蛾Spodoptera litura 3龄幼虫拒食活性。结果显示,氯仿萃取物比其他4种萃取物具有更高的拒食活性,在25 mg/mL的浓度下,24 h和48 h的拒食率分别为55.62%和52.66%。氯仿萃取物经硅胶柱层析后,得到14个组分。比较14个组分的拒食活性,发现组分4和10为主要的活性组分。这两个组分对斜纹夜蛾幼虫中肠脂肪酶和α-淀粉酶的活性都具有一定的抑制活性,其中组分10对脂肪酶具有显著的抑制效果,以1 mg/mL的浓度活体处理48 h和72 h抑制率分别达19.82％和34.60％; 对α-淀粉酶的影响在48 h和72 h内都具有显著的抑制效果,随着处理时间的延长,其抑制率逐步提高,72 h的抑制率分别为25.06％和27.40％。结果提示斜纹夜蛾幼虫中肠脂肪酶和α-淀粉酶可能是斑点野生稻拒食活性成分的作用靶标。     

斜纹夜蛾铁硫亚基蛋白的表达及功能鉴定

铁硫亚基蛋白(rieske iron-sulfur protein, RISP) 是线粒体复合物Ⅲ的关键蛋白亚基之一, 在呼吸链电子传递过程中起到重要作用。本研究通过RT-PCR克隆得到斜纹夜蛾Spodoptera litura RISP基因SlitRISP的ORF, 构建了pET32a-SlitRISP原核表达载体, SDS-PAGE和Western blot检测结果显示, SlitRISP原核表达蛋白以包涵体的形式存在于菌体沉淀中, 且RISP抗体可成功用于该蛋白的免疫印迹检测。为了进一步鉴定SlitRISP在斜纹夜蛾离体细胞系SL-1中的功能, 通过向细胞内转染siRNA, 利用RNAi技术沉默SL-1中的SlitRISP。qRT-PCR结果表明, 分别经50 nmol/L和100 nmol/L siRNA处理48 h后, SL-1中SlitRISP的表达均几乎完全被抑制; Western blot结果显示, SL-1中SlitRISP含量显著低于CK。当SL-1 SlitRISP被成功沉默后, 通过检测SL-1线粒体膜电位、 细胞ATP含量和细胞增殖抑制率鉴定RISP在线粒体电子传递过程中的重要作用。流式细胞仪测定结果表明, 经50 nmol/L和100 nmol/L siRNA处理24 h后, SL-1线粒体膜电位相对于CK分别降低23.52%和11.32%, 而处理48 h后, SL-1线粒体膜电位则分别升高5.58%和27.66%; siRNA处理24 h和48 h后, SL-1 ATP含量相对于CK分别降低82.71%和84.50%, 最终导致SL-1细胞增殖抑制率分别为53.64%和67.94%。这些结果表明SlitRISP在SL-1中参与线粒体膜电位的形成和细胞ATP的合成。介于RISP在线粒体电子传递链中的重要作用, 其可能成为新型杀虫作用靶标, 这可为研制新型呼吸抑制剂提供参考。     

Exposure to herbicides reduces larval sensitivity to insecticides in Spodoptera litura (Lepidoptera: Noctuidae)

Herbicides and insecticides are widely used in modern agriculture. It has been reported in various studies that application of insecticides can increase tolerance of herbivorous insects to insecticides. However, limited information exists on susceptibility to insecticides when insects are exposed to herbicides. This study was conducted to investigate the potential impact of the herbicides trifluralin and 2-methyl-4-chlorophenoxyacetic acid sodium salt (MCPA-Na) on the susceptibility of the nocturnal moth Spodoptera litura to the insecticides X-cyhalothrin, phoxim and bifenthrin. We found that larvae exposed to trifluralin or MCPA-Na became significantly less susceptible to both insecticides than nonexposed control larvae. Herbicide-treated larvae did not show altered growth under the used test conditions. However, heads of herbicide-treated larvae showed increased expression of the acetylcholinesterase genes SI Ace I and SI Ace 2. Moreover, the fat body and midgut of herbicide-treated larvae displayed elevated expression of detoxification genes (the carboxylesterase gene SI CarE;the glutathione S-transferase genes SlGSTe2 and SlGSTe3\ the cytochrome P450 monooxygenase genes CYP6B48, CYP9A40 and CYP321B1). The CYP6B48 gene exhibited highest inducibility. In conclusion, the data of this study suggest that exposure of S. litura larvae to herbicides may stimulate detoxification mechanisms that compromise the efficacy of insecticides.     

斜纹夜蛾蜕皮激素受体与超气门蛋白的原核表达及活性检测

双酰基肼类杀虫剂模拟天然蜕皮激素作用影响幼虫蜕皮。昆虫蜕皮激素受体的高度敏感性和专一性要求必须建立新的杀虫活性检测技术, 以适应快速准确和大批量筛选的要求。本研究采用RT-PCR技术, 获取斜纹夜蛾Spodoptera litura蜕皮激素受体（EcR）与超气门蛋白（USP）功能域目的基因, 构建EcR、 USP功能区基因原核表达载体（pEHISEGFPTEV-EcR_cde和pEHISEGFPTEV-USP_cde）。载体经诱导表达和蛋白纯化, 获得EcR和USP功能区纯化蛋白。在蛋白浓度l mg/mL, ³H-PonA终浓度8 nmol/L的条件下, 采用放射性配基受体结合分析测定了4种药剂（虫酰肼、 呋喃虫酰肼、 抑食肼和灭幼脲）不同浓度下的放射性比活的变化。结果显示： 随着药剂浓度的逐渐增大, 前3种药剂的放射性比活都有不同程度的降低, 其中虫酰肼的放射性比活降低程度最大, 其次是呋喃虫酰肼和抑食肼, 灭幼脲的放射性比活基本无变化。这些结果表明相同条件下虫酰肼比呋喃虫酰肼和抑食肼有更高的杀虫活力, 本研究的方法可对双酰基肼类杀虫剂或者先导化合物进行初步筛选。     

Identification and localization of two sensory neuron membrane proteins from Spodoptera litura (Lepidoptera: Noctuidae)

Sensory neuron membrane proteins (SNMPs), which are located on the dendritic membrane of olfactory sensory neurons (OSNs), are proposed to be associated with odor reception in insects. Recent studies have demonstrated that SNMP1 is essential for electrophysiological responses of OSNs to the sex pheromone, cis‐vaccenyl acetate (cVA) in Drosophila melanogaster. To investigate the function of Lepidoptera SNMPs, we cloned two SNMP genes, SlituSNMP1 and SltiuSNMP2, from Spodoptera litura (Lepidoptera: Noctuidae). Sequence alignment and phylogenetic analysis showed that both genes bear the general characteristics of SNMPs, including six conserved cysteine residues and two transmembrane domains. Further expression profile experiments showed that SlituSNMP1 is mainly expressed in the antenna, while SlituSNMP2 is broadly expressed in various tissues. By in situ hybridization experiments, it was found that SlituSNMP1 expressing cells are surrounded by the SlituSNMP2 expressing cells in the pheromone sensitive sensilla, suggesting different functions of the two SNMPs in insect olfaction.     

斜纹夜蛾抗性种群中酶抑制剂对杀虫剂的增效作用（英文）

采用浸液生测法研究了斜纹夜蛾Spodoptera litura巴基斯坦抗性种群中酶抑制剂［胡椒基丁醚（PBO）和脱叶膦（DEF)］对丙溴磷、灭多威、硫双灭多威、氯氰菊酯、氯氟氰菊酯、联苯菊酯、茚虫威和多杀菌素等杀虫剂的增效作用。结果表明：PPO和DEF对氨基甲酸酯杀虫剂灭多威和硫双灭多威均具有增效作用,但对有机磷杀虫剂丙溴磷不具有增效作用。两种抑制剂对氯氰菊酯均产生增效作用,但对联苯菊酯没有增效作用。PPO 和DEF增加了氯氟氰菊酯对Multan种群的毒性,但没有增加其对Mailsi种群的毒性。DEF对多杀菌素具有增效作用,但PBO对其没有增效作用。PBO和DEF对氨基甲酸酯杀虫剂、拟除虫菊酯杀虫剂、茚虫威和多杀菌素具有明显的增效作用,这说明细胞色素P450单加氧酶和酯酶的解毒作用至少部分参与了斜纹夜蛾对这些杀虫剂的抗性过程。不过,两种增效剂对杀虫剂增效作用范围有限,暗示对于斜纹夜蛾巴基斯坦种群而言,其他的机制（如靶位点不敏感、表皮穿透作用降低）可能是更重要的抗性机制。     

铅胁迫对斜纹夜蛾生长发育与生殖的影响

在植食性昆虫斜纹夜蛾幼虫标准人工饲料中添加不同浓度的重金属铅(Pb),研究Pb胁迫对其生长发育与生殖的影响.结果表明:斜纹夜蛾不同发育阶段(幼虫、蛹和成虫)的存活率和体质量随着饲料中Pb浓度的增加而下降,引起存活率显著下降的最低Pb胁迫浓度是100 mg·kg-1,引起体质量显著减少的最低Pb胁迫浓度是50 mg·kg-1.在取食Pb浓度为25～ 200 mg·kg-1的人工饲料后,斜纹夜蛾成虫的产卵天数显著减少;产卵力和生育力随着饲料中Pb浓度的增加而显著下降;1000粒卵的平均质量显著低于对照;卵孵化率显著下降.重金属Pb胁迫对斜纹夜蛾生长发育和生殖具有显著的抑制作用.     

斜纹夜蛾两个天蚕素D基因的克隆及序列分析

天蚕素是昆虫抵御病菌入侵的一类抗菌肽家族。根据斜纹夜蛾Spodoptera litura天蚕素B基因设计特异引物,通过PCR扩增得到2个新的天蚕素基因部分序列,分别命名为cecD1和cecD2（GenBank登录号分别为EF555567和EF555568）。2个基因编码同一个天蚕素D蛋白,该蛋白的成熟肽与天蚕素B存在2个氨基酸残基差异。序列分析发现cecD1和cecD2中分别包含568 bp和377 bp的内含子序列,它们有相同的5′和3′拼接位点,A+T含量分别为59.7%和69.8%,符合大多数真核生物内含子高A+T含量的特征。     

斜纹夜蛾性信息素通讯系统

采用单雌腺体微量分析技术,对斜纹夜蛾(中国种群)雌蛾腺体的组份进行鉴定,并研究了各组份的个体差异及释放规律,测试了雄蛾对各组份及其混合物的触角电位反应。雌蛾腺体内含有4个组份：Z9, E11-14∶Ac(A)、Z9, E12-14∶Ac(B)、Z9-14∶Ac© 和E11-14∶Ac(D),其比例为100∶27∶20∶27。     

三种阔叶草坪草对斜纹夜蛾生长发育及营养利用的作用

为了探讨斜纹夜蛾Spodoptera litura对草坪的为害,提供综合治理该虫的理论依据,室内利用白三叶草Trifolium repens、红三叶草T. pratense和马蹄金Dichondra repens 3种阔叶草坪草,分别饲喂斜纹夜蛾幼虫;应用编制实验种群生命表的方法,分析斜纹夜蛾种群生长发育的趋势;同时研究该幼虫对3种草坪草的趋性及营养利用。结果表明：斜纹夜蛾幼虫的趋性表现为白三叶草＞红三叶草＞马蹄金。该虫在3种草坪草上都能够完成生活史;2种三叶草饲喂的该虫幼虫存活率、化蛹率、成蛾率显著高于马蹄金,幼虫期和蛹期则显著短于马蹄金;3种草坪草饲喂的该虫产卵期和平均产卵量相互之间差异不显著,而马蹄金饲喂的产卵前期和雌成虫寿命则显著短于2种三叶草。3种草坪草饲喂的该虫净生长率（Ro）、世代平均周期（T）、内禀生长率（r_m）、周限生长率（λ）、种群加倍时间（DT）及种群增长参数（I）都有差异,其中2种三叶草的各种群增长参数较接近。对5龄幼虫取食3种阔叶草坪草的营养指标测定结果显示：取食马蹄金的相对生长率显著高于取食红三叶草的;取食马蹄金的相对取食量和近似消化率显著高于取食三叶草的;取食马蹄金的食物利用率和食物转化率显著低于取食三叶草的;2种三叶草间无显著差异。综合分析认为,斜纹夜蛾最喜食三叶草,最容易在三叶草草坪上猖獗为害。