共查询到20条相似文献,搜索用时 46 毫秒
1.
Bhatt D Cole SP Grabar TB Claggett SB Cain BD 《Journal of bioenergetics and biomembranes》2005,37(2):67-74
The peripheral stalk of F1F0 ATP synthase is composed of a parallel homodimer of b subunits that extends across the cytoplasmic membrane in F0 to the top of the F1 sector. The stalk serves as the stator necessary for holding F1 against movement of the rotor. A series of insertions and deletions have been engineered into the hydrophilic domain that interacts with F1. Only the hydrophobic segment from {val-121} to {ala-132} and the extreme carboxyl terminus proved to be highly sensitive to mutation. Deletions in either site apparently abolished enzyme function as a result of defects is assembly of the F1F0 complex. Other mutations manipulating the length of the sequence between these two areas had only limited effects on enzyme function. Expression of a b subunit with insertions with as few as two amino acids into the hydrophobic segment also resulted in loss of F1F0 ATP synthase. However, a fully defective b subunit with seven additional amino acids could be stabilized in a heterodimeric peripheral stalk within a functional F1F0 complex by a normal b subunit. 相似文献
2.
Cytoplasmic male sterility (CMS) has often been associated with abnormal mitochondrial open reading frames (ORF), orfH79 is a mitochondria chimeric gene being responsible for the CMS trait in Honglian (HL) rice. Weakly expressed ORFH79 strongly
inhibits the growth of yeast cells. In addition, the content of reactive oxygen species (ROS) in the transformants that expressed
ORFH79 was increased by 31%, and ATP was decreased by 41% compared with the control. These results showed ORFH79 peptide is
toxic to yeast cells. 相似文献
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Giovanna Lippe Elena Bisetto Marina Comelli Stefania Contessi Francesca Di Pancrazio Irene Mavelli 《Journal of bioenergetics and biomembranes》2009,41(2):151-157
Mitochondria are central to heart function and dysfunction, and the pathways activated by different cardioprotective interventions
mostly converge on mitochondria. In a context of perspectives in innate and acquired cardioprotection, we review some recent
advances in F0F1ATPsynthase structure/function and regulation in cardiac cells. We focus on three topics regarding the mitochondrial F0F1ATPsynthase and the plasma membrane enzyme, i.e.: i) the crucial role of cardiac mitochondrial F0F1ATPsynthase regulation by the inhibitory protein IF1 in heart preconditioning strategies; ii) the structure and function of mitochondrial F0F1ATPsynthase oligomers in mammalian myocardium as possible endogenous factors of mitochondria resistance to ischemic insult;
iii) the external location and characterization of plasma membrane F0F1 ATP synthase in search for possible actors of its regulation, such as IF1 and calmodulin, at cell surface. 相似文献
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Pathania A Kumar R Kumar VD Ashutosh Dwivedi KK Kirti PB Prakash S Chopra VL Bhat SR 《Journal of genetics》2007,86(2):93-101
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Bisetto E Picotti P Giorgio V Alverdi V Mavelli I Lippe G 《Journal of bioenergetics and biomembranes》2008,40(4):257-267
The role of the integral inner membrane subunit e in self-association of F0F1ATP synthase from bovine heart mitochondria was analyzed by in situ limited proteolysis, blue native PAGE/iterative SDS-PAGE, and LC-MS/MS. Selective degradation of subunit e, without disrupting
membrane integrity or ATPase capacity, altered the oligomeric distribution of F0F1ATP synthase, by eliminating oligomers and reducing dimers in favor of monomers. The stoichiometry of subunit e was determined
by a quantitative MS-based proteomics approach, using synthetic isotope-labelled reference peptides IAQL*EEVK, VYGVGSL*ALYEK,
and ELAEAQEDTIL*K to quantify the b, γ and e subunits, respectively. Accuracy of the method was demonstrated by confirming
the 1:1 stoichiometry of subunits γ and b. Altogether, the results indicate that the integrity of a unique copy of subunit
e is essential for self-association of mammalian F0F1ATP synthase.
Elena Bisetto and Paola Picotti contributed equally to this work. 相似文献
8.
Li S Tan Y Wang K Wan C Zhu Y 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(8):1389-1397
For years discovery and identification of the cytoplasmic male sterility (CMS) resource in wild rice is the most intriguing
events in breeding field. orfH79, a chimeric gene in mitochondria, has been suggested being the determinant for Honglian CMS in rice. In this report orfH79 gene as molecular marker to screen the wild rice, we found eight accessions with orfH79 gene in the total 42 investigated objects. Sequence analysis revealed that there were a total of nine nucleotide substitutions
resulting in the change of nine amino acids in the newly identified orfH79 in wild rice, which further fell into seven haplotypes. In order to investigate the underlying relationship between orfH79 haplotypes and the corresponding fertility restorers, four accessions were selected with different orfH79 haplotype as female parents to hybridize the Honglian maintainer line, Yuetai B. After eight consecutive recurrent backcrosses,
four alloplasmic CMS lines with different orfH79 haplotype were developed. Microscopic observation exhibited that their pollen grains were spherical and clear in 1% I2–KI solution same as that of Honglian CMS line. Moreover, these four CMS lines displayed various fertility restoring model
through test cross, suggesting that each orfH79 haplotye represents a new CMS type and corresponds to their specific Rf allele. 相似文献
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In Escherichia coli, the F1FO ATP synthase b subunits house a conserved arginine in the tether domain at position 36 where the subunit emerges from the membrane. Previous
experiments showed that substitution of isoleucine or glutamate result in a loss of enzyme activity. Double mutants have been
constructed in an attempt to achieve an intragenic suppressor of the b
arg36→ile and the b
arg36→glu mutations. The b
arg36→ile mutation could not be suppressed. In contrast, the phenotypic defect resulting from the b
arg36→glu mutation was largely suppressed in the b
arg36→glu,glu39→arg double mutant. E. coli expressing the b
arg36→glu,glu39→arg subunit grew well on succinate-based medium. F1FO ATP synthase complexes were more efficiently assembled and ATP driven proton pumping activity was improved. The evidence
suggests that efficient coupling in F1FO ATP synthase is dependent upon a basic amino acid located at the base of the peripheral stalk. 相似文献
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Bagramyan K Mnatsakanyan N Trchounian A 《Biochemical and biophysical research communications》2003,306(2):361-365
Escherichia coli growing on glucose under anaerobic conditions at slightly alkaline pH carries out a mixed-acid fermentation resulting in the production of formate among the other products that can be excreted or further oxidized to H(2) and CO(2). H(2) production is largely dependent on formate dehydrogenase H and hydrogenases 3 and 4 constituting two formate hydrogen lyases, and on the F(0)F(1)-ATPase. In this study, it has been shown that formate markedly increased ATPase activity in membrane vesicles. This activity was significantly (1.8-fold) stimulated by 100mM K(+) and inhibited by N,N(')-dicyclohexylcarbodiimide and sodium azide. The increase in ATPase activity was absent in atp, trkA, and hyf but not in hyc mutants. ATPase activity was also markedly increased by formate when bacteria were fermenting glucose with external formate (30mM) in the growth medium. However this activity was not stimulated by K(+) and absent in atp and hyc but not in hyf mutants. The effects of formate on ATPase activity disappeared when cells were performing anaerobic (nitrate/nitrite) or aerobic respiration. These results suggest that the F(0)F(1)-ATPase activity is dependent on K(+) uptake TrkA system and hydrogenase 4, and on hydrogenase 3 when cells are fermenting glucose in the absence and presence of external formate, respectively. 相似文献
13.
Mitochondrial F(1)F(0)-ATPase is a key enzyme in plant metabolism, providing cells with ATP that uses the transmembrane electrochemical proton gradient to drive synthesis of ATP. A 6 kDa protein (At3g46430) has been previously purified from Arabidopsis thaliana mitochondrial F(1)F(0)-ATPase. In this study, the gene (AtMtATP6; GenBank accession no. AK117680) encoding this protein was isolated from Arabidopsis and characterized. Northern blot analyses showed that the expression of AtMtATP6 gene in Arabidopsis suspension-cultured cells was induced by several abiotic stresses from salts, drought, and cold. Over-expression of AtMtATP6 gene in transgenic yeast and Arabidopsis plants increased the resistance to salts, drought, oxidative and cold stresses. Taken together, our data raise the possibility that induction of the F(1)F(0)-ATPase plays a role in stress tolerance. 相似文献
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Xiaofang Liao Yanhong Zhao Peng Chen Bujin Zhou Yong Diao Mingli Yu Zhipeng Huang Ruiyang Zhou 《Plant Molecular Biology Reporter》2016,34(1):29-36
In this study, the atp8 gene was cloned from the cytoplasmic male sterile (CMS) line UG93A and its maintainer line UG93B in kenaf. Its DNA sequence analysis showed that atp8 containing 480-bp, encoding 159 amino acid residues, and a 9-bp insertion was found at the 3′flanking sequence in UG93A compared with UG93B. The cDNA sequence of atp8 analyzed by RT-PCR indicated that there were five loci edited, but six loci edited in UG93B. The editing frequencies were higher in sterile cytoplasm than in fertile cytoplasm. The relative expression of atp8 analyzed by real-time PCR showed that the expressed level of atp8 in UG93A was lower than that of its maitainer UG93B and its F1 hybrid UG93A/992 (a restore line). Furthermore, based on the difference of the 9-bp differences at the 3′flanking sequence of atp8 between UG93A and UG93B, a molecular marker specific to male sterile cytoplasm was developed, which can be used for indentifying whether any germplasm of kenaf is male sterile cytoplasm or male fertile cytoplasm. 相似文献
16.
The nucleotide sequences of the genes encoding the F1F0-ATPase beta-subunit from Oenococcus oeni, Leuconostoc mesenteroides subsp. mesenteroides, Pediococcus damnosus, Pediococcus parvulus, Lactobacillus brevis and Lactobacillus hilgardii were determined. Their deduced amino acid sequences showed homology values of 79-98%. Data from the alignment and ATPase tree indicated that O. oeni and L. mesenteroides subsp. mesenteroides formed a group well-separated from P. damnosus and P. parvulus and from the group comprises L. brevis and L. hilgardii. The N-terminus of the F1F0-ATPase beta-subunit of O. oeni contains a stretch of additional 38 amino acid residues. The catalytic site of the ATPase beta-subunit of the investigated strains is characterized by the two conserved motifs GGAGVGKT and GERTRE. The amplified atpD coding sequences were inserted into the pCRT7/CT-TOPO vector using TA-cloning strategy and transformed in Escherichia coli. SDS-PAGE and Western blot analyses confirmed that O. oeni has an ATPase beta-subunit protein which is larger in size than the corresponding molecules from the investigated strains. 相似文献
17.
Cytoplasmic male sterility (CMS) is an important trait in rice (Oryza sativa L.) breeding because it provides a source for producing hybrid seeds. In rice CMS lines, ATPases involved in the oxidative
phosphorylation complexes are believed to be dysfunctional due to the expression of rice CMS-related gene orf79. In the present study, a new type of CMS line named CMS-ZA (ZidaoA) was used. We found an orf79 homologous gene (named orfZ79) in three different rice lines (a CMS line, a maintainer line, and a hybrid). However, no detectable expression products
of orfZ79 were found in the three lines. We evaluated the ATPase and NADH dehydrogenase activities of the three lines using in-gel
catalytic assays. Our results show that the sterile line has intact ATPase activity, while NADH DHase activity is clearly
decreased. To investigate NADH dehydrogenase deficiency, we measured NADH DHase activity in etiolated seedlings and green
seedlings from the ZidaoA CMS sterile line and its corresponding maintainer line. We note that the NADH DHase activity of
the sterile line was more deficient in green seedlings than that in etiolated seedlings. Our results show a possible role
of NADH DHase deficiency to cause rice CMS. 相似文献
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G. R. Rodríguez L. Sequin G. R. Pratta R. Zorzoli L. A. Picardi 《Biologia Plantarum》2008,52(3):548-552
Pericarp polypeptide profiles were analyzed at three ripening stages in the F1 hybrid and the F2 population from the cross between the accessions: LA1385 (Lycopersicon esculentum var. cerasiforme) and 804627 (L. esculentum, a homozygous genotype for the nor mutant). Six polymorphic polypeptides were observed in LA1385, while no polymorphic polypeptides among ripening stages was
observed in 804627. On the other hand, some polypeptides in the F1 hybrid were not observed in the parents whereas others were present in both parental genotypes and were unnoticeable in the
hybrid genotype. From a cluster analysis on the protein profiles of the F2 population, the differential expression of proteins allowed to distinguish mature green (MG) stage from the others two stages,
while for breaker stage (BR) and red ripe stage, the genetic background was more important in forming groups. The differential
expression of proteins could be associated with fruit morphology traits such as a 72 kDa polypeptide present in MG stage with
fruit diameter, height and mass and a 47 kDa polypeptide found in BR with fruit shelf life. 相似文献