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Mathew A Mathur SK Jolly C Fox SG Kim S Morimoto RI 《Molecular and cellular biology》2001,21(21):7163-7171
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Arabidopsis heat shock factor: isolation and characterization of the gene and the recombinant protein 总被引:18,自引:0,他引:18
We have isolated the Arabidopsis heat shock factor gene Athsf1 as genomic and corresponding cDNA sequences via cross-hybridization with tomato clones. Sequence analysis indicates only a partial homology with the HSFs from tomato and other organisms which is confined to the DNA-binding and the oligomerization domains. The gene is constitutively expressed but the level of mRNA for Athsf1 increases two-fold upon heat shock. However, the putative promoter region lacks the canonical heat shock elements. After expression in Escherichia coli the recombinant Athsf1 protein binds specifically to a synthetic oligonucleotide containing five heat shock elements. The native size of recombinant ATHSF1 in vitro is consistent with a trimer as demonstrated by chemical cross-linking and pore exclusion limit analysis.The publication is dedicated to Professor Dr. Wolfram Heumann, Universität Erlangen, on occassion of his 80th birthday. 相似文献
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Activation of heat shock genes is not necessary for protection by heat shock transcription factor 1 against cell death due to a single exposure to high temperatures
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Inouye S Katsuki K Izu H Fujimoto M Sugahara K Yamada S Shinkai Y Oka Y Katoh Y Nakai A 《Molecular and cellular biology》2003,23(16):5882-5895