蓝胸秧鸡亚种新纪录

蓝胸秧鸡 Rallus striatus albiventer (Swainson) Rallus albiventer Swainson, 1838, Animals in Menageries: 337 (印度)。Aii, S., and S.D. Ripley 1980 Handbook of the birds of Indai and Pakistan. Vol. 2. P: 153—154.; Deignan, H.G. 1945 The birds of northern Thailand. P: 103—104.; Smythies, B.E. 1953 The birds of Burina. P: 452—454. and P; 609.     

苯丙氨酸羟化酶（PAH）基因外显子7及其两侧内含子的突变研究

为探讨中国苯丙酮尿症（PKU）人群中苯丙氨酸羟化酶（PAH）基因外显子7的突变特征,对147例PKU患儿的294个PAH基因外显子7以及两侧部分内含子序列,应用PCR－单链构象多态性（SSCP）分析及基因序列分析的方法进行了筛查和确定。共发现13种突变基因：G239D、R241C、R241fs、R243Q、G247S、G247V、R252Q、L255S、R261Q、M276K、E280G、P281L、Ivs7+2T>A,其中7 种突变基因在中国PKU人群首次发现：G239D 、R241fs 、G247S 、E280G、L255S、R261Q、P281L,前4种在国际上尚未见到报道,并已提交到国际PAH突变数据库（www.pahdb.mcgill.ca）。突变基因的总频率为30.61%（90 /294）。突变涉及了错义、缺失、移码和剪接位点4种突变类型。结果明确了PAH基因外显子7的突变种类和分布等特征,表明外显子7是中国人PAH基因突变的热点区域。 Abstract: To study mutation in exon 7 of the gene for the phenylalanine hydroxylase（PAH）, the mutations in exon 7 and flanking sequence of PAH gene were detected by means of SSCP analysis and DNA sequencing, in 147 unrelated Chinese children with phynelketonuria and their parents. Thirteen different mutations, including 11 missense, 1 deletion and 1 splice mutation, were revealed in 90/294 mutant alleles (30.61%). The prevalent mutations were R243Q (22.8%) and Ivs7nt2t->a (2.38%). Seven novel mutations were identified: G239D, R241fsdelG, G247S, E280G, L255S, R261Q, P281L. These new mutations have not been described in Chinese PKU population and the first 4 mutants have not been reported and thus been submitted to www.pahdb,mcgill.ca. The missense was the most common type. The deletion and frameshift mutations were detected for the first time in Chinese PKU population. This study showed the mutation characteristics and their distribution in exon 7 of PAH gene and proved that the exon 7 was the hot region of PAH gene mutation in Chinese PKU population .     

圈养金钱豹尿液中的挥发性成分分析

<正>哺乳动物的尿液挥发性成分被认为是同哺乳动物信息激素一样重要的物质,迄今报道的野生动物尿液挥发性成分研究的有狮(Panthera leo)(Andersen and Vulpius,1999)、山猫(Lynx rufus)(Mattina et al.,1991)、土狼(Canis lupus)(Raymer et al.,1986)、郊狼(Canis latrans)(Schultz,1988)、红狐(Vulpes vulpe)(Jorgensen et al.,1978)以及鼬科物种(Zhang et al.,2005)。Robert和Joseph(1991)利用山猫和土狼尿液气味抑制白尾鹿(Odocoileus leucurus)对日本     

Transfer of small chromosome fragments of Agropyron elongatum to wheat chromosome via asymmetric somatic hybridization

~~Transfer of small chromosome fragments of Agropyron elongatum to wheat chromosome via asymmetric somatic hybridization1 .Dong,Y.C,GenePools of common wheat,Journal of Triticeae CroPs(in Chinese),2000,20(3):78-81. 2 .Wei,Y.M.,Zheng,YL.Zhou,R.H., Detectlon of the rye chro- matin in multisPikelet wheat germplasm 10-A background using fluorescence in situ hybridization(FISH)and RFLP markers,Acta Bot.Sinica(in Chinese),1999,41(7):722-725. 3 .Xiang,E N.,Xia,G M.…     

Acute paralysis viruses of the honey bee

<正>The alarming decline of honey bee(Apis mellifera)colonies in the last decade drove the attention and research to several pathogens of the honey bee including viruses.Viruses challenge the development of healthy and robust colonies since they manage to prevail in an asymptomatic mode and reemerge in acute infections following external stresses,as well as they are able to infect new healthy colonies(de Miranda J R,et al.,2010a;de Miranda J R,et al.,2010b;Di Prisco G,et al.,2013;Nazzi F,et al.,2012;Yang X L,et al.,2005).     

犬蝠夜栖息地及夜栖息巢特征的初步研究

<正>大多数种类的蝙蝠不会整个晚上都进行觅食,通常在觅食期间有一段长短不一的时间停留在临时地休息,此为夜栖息行为(Hatfield,1937;Krutzsch,1954;Barbour and Davis,1969;Kunz,1973,1974;Hirshfeld et al.,1977)。蝙蝠在夜栖息地进食(Vaughan,1976;Funakoshi and Maeda,2003)、休息并消化食物(Brigham,1991;Funakoshi and Maeda,2003),甚至社会交流(Kunz,1982;Kunz and Lumsden,2003)。不同种类的蝙蝠     

土耳其斯坦东毕吸虫的扫描电镜观察

血吸虫类中如血居科(sanguinicolidae)的Aporocotyle simplex Odhner,1900、裂体科(Schistosomatidae)的日本血吸虫(Schistosoma japonicum)曼氏血吸虫(S.mansoni)及埃及血吸虫(S.haematobium)等多种血吸虫均经扫描电镜观察(Johnson and Moriearts,1969;Silk et al., 1969;Robson and Erasmus, 1970; Miller et al., 1972; Kuntz et al.,1976、1977;Voge et al., 1978; Thulin, 1980;及何毅勋和马金鑫,1980等)。关于土耳其斯坦东毕吸虫[Orientobitharzia lurkestanica(skrjabin,1913)Dut et Srivastava,1955]的体表扫描电镜尚无报告,而只见有此虫种体壁及肠管的透射电镜观察的资料(Lavrov and Fedoseenke,1978)。本文部分作者最近在内蒙东部兴安岭以南部分地区进行牛羊土     

ORDOVICIAN NAUTILOID FAUNA FROM LUNSHAN, JURONG, JIANGSU

The fossil nautiloids described in this paper were collected in 1984 and 1985 from Lunshan, Jurong in Jiangsu; there the Ordovician strata are well developed and may be divided in descending order into seven formations as follows:Upper Ordovician: Wufeng Formation Tangtou FormationMiddle Ordovician Pagoda Formation Lower OrdovicianKuniutan FormationDawan FormationHunghuayuan FormationLunshan FormationThese nautiloids comprise 36 species belonging to 13 genera (including one new genus and 11 new species), namely,Middle OrdovicianPagoda FormationSinoceras chinense (Foord), Michelinoceras changjiangense Chen, densn (Y), M. paraelongatum Chang.Lower OrdovicianKuniutan FormationRhynchorthoceras jurongense sp. nov., R. lunshanense sp. nov., Dideroceras wahlenbergi (Foord), D. holmi Flower, Michlinoceras irregulare sp. nov., Kotoceras gaoluncunense sp. nov.Dawan Formation.Cochlioceras yantzeense Chang, Chisiloceras ichangense Wang, C. yaoshanense Qi, C. changyangense (Chang), Protocycloceras deprati Reed, P. hupehcnse (Shimizu and Obata), P. sichuanense Wang, P. remotum Lai, P. gangshanense sp. nov., P. siphosedimentum Pan, P. chaoi sp. nov., P.shishantouense sp. nov., P. gaojiabianense sp. nov., Changyangoceras gaojiabianense sp. nov., Gangshanoceras jurongense gen. et sp. nov., G. densum gen. et sp. nov., Michelinoceras cf. dayongense Lai, Troedssonella lunshanensis sp. nov., Mesosendoceras leei (Y), Vaginoceras peiyangense malukonense Chen, Dideroceras mui (Chang), D. gaoluncunense sp. nov.     

A new species of Viscum (Viscaceae) from Bandipur,Karnataka State, India

Viscum bandipurense M.C.Thriveni,G.R.Shivamurthy,K.N.Amruthesh,C.R.Vijay & K.B.Sadanand,sp.nov. (S2-2.Fig.1,S2-3.Fig.2) Type:India.Karnataka:Chamarajanagar District,BandipurNational Park,75 ° 12′ 17″ E to 76°51′32″ E and 11 °35′34″ N to 11 °57′02″ N,2010-09-10,M.C.Thriveni 181 (holotype,CAL;isotype,MUH).     

Identification and expression profiling of putative odorant-binding proteins in the malaria mosquitoes, Anopheles gambiae and A.arabiensis

~~Identification and expression profiling of putative odorant-binding proteins in the malaria mosquitoes, Anopheles gambiae and A. arabiensis1. Curtis, C. F., Introduction 1: An overview of mosquito biology, behaviour and importance, in Olfaction in Mosquito-Host Interactions (eds. Bock, G. R.. Cardew, G.), New York: Wiley, 1996, 3-7. 2. Nighom, A., Hildebrand. J. G.. Dissecting the molecular mechanisms of olfaction in a malaria-vector mosquito, PNAS, 2002, 99(3): 1113-…     

3’-UTR sequence of Macrobrachium rosenbergii extra small virus(XSV) is important for viral RNA packaging

正Dear Editor,The packaging of viral genomic RNA into virus par-ticles is a critical step for virus maturation.This stepincludes the recognition and interaction between the nu-cleocapsid protein and viral RNA.The necessity of viralRNA packaging signals has been described for manyRNA viruses(Cologna R,et al.,2000;Narayanan K,et al.,2001;Tchatalbachev S,et al.,2001).Occasionalpackaging of nongenomic viral RNAs and cellular RNAs     

分子生物学新书简介

(一)《分子和亚细胞生物进展》(Progress in Molecular and Sabcellular Biology)丛书于1969年出版第一本书,1988年出版了第10本,从第1本至第10本均有馆藏,是一套信息量比较齐全的分子生物学研究丛书。该书作者是P. Jeantear, Y. Kuchino W. E. G. Mlter和P. L. Paine。由Springer—Verlag出版     

《农业生态与环境》简介

《农业生态与环境》(Agricultural Ecology and Environment)由M. G. Paoletti、B. R. Stinner和G. G. Lorenzoni三位学者共同编著,此书于1989年由荷兰阿姆斯特丹的Elsevier科学出版社出版。该书是1988年4月在意大利帕多瓦市举行的“农业与环境国际学术讨论会”论文汇编,是     

Fox- and raccoon-dog-associated rabies outbreaks in northern China

<正>Dear Editor,Rabies is a generally fatal disease caused by the rabies virus(RABV),and is transmitted mainly by Carnivora and Chiroptera(Fooks A R,et al.,2014;Tao X,et al.,2013).In China,stray dogs and some wild animals(e.g.,Chinese ferret badgers,foxes,and raccoon dogs)are the principal reservoirs for RABV(Hu R L,et al.,2009).Historically,rabies in wild foxes and raccoon dogs(Nyctereutes procyonoides)was recorded in the early     

大鳞副泥鳅雄核单倍体的早期发育

目前,有关鱼类雄核发育的研究已见于川鲽(Platichthys flesus)、马苏大麻哈鱼(Oncorhynchus masou)、虹鳟(Oncorhynchus mykiss)、红点溪鳟(Salvelinus fontinalis)、鲤鱼(Cyprnus carpio)、尼罗罗非鱼(Oreochromis niloticus)、泥鳅(Misgurnus anguillicaudatus)、斑马鱼(Danio rerio)等(Purdom,1969;Arai et al., 1979; Scheerer et al., 1991; May et al., 1988; Bongers et al., 1994;Grunina et al., 1990; Meyers, 1995; Masaoka et al., 1995; 赵振山等,1998;Corley-Smith et al., 1996).     

建议进一步阅读的书刊

1、一般教科书 Lehninger,A .L.(2975),Bioehe。￡冬tr夕,Znd edit三on,Worth Pt:blishers,New York. Morris,J.G.(1974),A Biolo夕‘s考sPh夕、ie:IC几e。玄str夕,2。改editio:,Ed,ard A rnold,London. 5 tanier,R.Y.,Adelburg,E.A.and ingraham,J.L.(1977),G。。。ral对‘ero- biolo夕y,4th edition,Prentiee Hall,New Jersey.2.专门读物 Barrett,J.and Lemberg,R.(1973),C夕toeh,ooe:,Aeademie Press,London. Chappell,J.B.(2977),ATp,Carolina Biology Readers,Burlington Gregory,R.P.F(1974),B‘Ooh:。￡str夕o…     

Intracellular distribution, assembly and effect of disease-associated connexin 31 mutants in HeLa cells

Mutations in connexin 31 （Cx31） are associated with erythrokeratodermia variabilis （EKV）,hearing impairment and peripheral neuropathy; however, the pathological mechanism of Cx31 mutants remains unknown. This study analyzed 11 disease-associated Cx31 variants and one non-disease-associated Cx31 variant and compared their intracellular distribution and assembly in HeLa cells and their effect on these cells. The fluorescent localization assay showed no gap junction plaque formation in the cells expressing the recessive EKV-associated mutant （L34P） and four hearing impairment-associated mutants （66delD,141delI, R180X and E183K）, significantly reduced plaque formation in the cells with five EKV-associated dominant mutants （G12R, G12D, R42P, C86S and F137L） and no obvious change in the cells with two other mutants （I 141V and 652del 12）. Immunoblotting analysis showed that 12 mutated Cx31 s, like WTCx31, are able to form the Triton X-100 insoluble complex; however, the quantity of Triton X-100 insoluble complex in the transfected HeLa cells varied among different Cx31 mutants. Additionally, the expression of five EKV-associated dominant mutants （G12R, G12D, R42P, C86S and F137L） caused cell death in HeLa cells. However, the five heating impairment-associated mutants did not induce cell death. The above results suggest that disease-associated mutants gain deleterious functions differentially. In summary, diseaseassociated Cx31 mutants impair the formation of normal gap junctions at different levels, and the diseases associated with Cx31 mutations may result from the abnormal assembly, trafficking and metabolism of the Cx31 mutants.     

Fox- and raccoon-dog–associated rabies outbreaks in northern China

正Dear Editor,Rabies is a generally fatal disease caused by the rabies virus(RABV),and is transmitted mainly by Carnivora and Chiroptera(Fooks A R,et al.,2014;Tao X,et al.,2013).In China,stray dogs and some wild animals(e.g.,Chinese ferret badgers,foxes,and raccoon dogs)are the principal reservoirs for RABV(Hu R L,et al.,2009).Historically,rabies in wild foxes and raccoon dogs(Nyctereutes procyonoides)was recorded in the early     

Physiological response of eight Mediterranean maquis species to low air temperatures during winter

We analyzed the physiological response of the Mediterranean evergreen species (Arbutus unedo L., Cistus incanus L., Erica arborea L., Erica multiflora L., Phillyrea latifolia L., Pistacia lentiscus L., Quercus ilex L., and Rosmarinus officinalis L.) to winter low air temperatures. In occasion of two cold events, in February 2005 (T _min = 1.8 °C), and January 2006 (T _min = 3.1 °C and minimum T _air = −0.40 °C during the nights preceding the measurements), R. officinalis, C. incanus, and E. multiflora had the highest net photosynthetic rate (P _N) decrease (73 %, mean value) with respect to the winter P _N maximum, followed by A. unedo (62 %), P. latifolia and P. lentiscus (54 %, mean value), E. arborea (49 %), and Q. ilex (44 %). Among the considered species, Q. ilex was able to maintain P _N near the maximum for 150 min during the day, A. unedo, P. lentiscus, E. arborea, P. latifolia, E. multiflora, and R. officinalis for 60 min, and C. incanus for 30 min. The calculated mean winter daily P _N ranged from 7.9±0.6 (Q. ilex) to 2.8±0.5 (R. officinalis) μmol(CO₂) m⁻² s⁻¹. During the study period, chlorophyll (Chl) content decreased by 36 % on an average in the two cold events, and the carotenoid (Car) to Chl ratio increased by 133 % in Q. ilex, having the highest value in January 2006. Principal component analysis underlined the highest cold resistance of Q. ilex by high P _N and high Car/Chl ratio. On the contrary, R. officinalis and C. incanus had the lowest cold resistance by the highest P _N decrease and the lowest Car/Chl (C. incanus). Thus, winter stress could be an additional limitation to Mediterranean evergreen species production, and the capacity of the species to maintain P _N near 90–100 % during winter is determinant for biomass accumulation.