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1.
Favre TC Ximenes RA Galvão AF Pereira AP Wanderlei TN Barbosa CS Pieri OS 《Memórias do Instituto Oswaldo Cruz》2006,101(Z1):73-78
The Program for Schistosomiasis Control within the Unified Health System (PCE-SUS) was implemented by 1999 in the Rainforest Zone or "Zona da Mata" of Pernambuco (ZMP) aiming to carry out biennial stool surveys of whole populations through municipal health organs followed by treatment of the positives through the local units of the Family Health Program (PSF). Yearly reports from the Health Department of Pernambuco State (SES/PE) from 2002 to 2004 on the PCE-SUS surveys were assessed to evaluate whether the current estimates of prevalence in the municipalities of the ZMP are based on reliable samples so as to allow considerations on the real situation of schistosomiasis in that area. The surveys carried out in that period did not follow the major principles underlying sampling design, thus posing problems in both precision and validity of the estimates. Only 12 out of 43 municipalities had minimally reliable estimates: five with moderate prevalence (10-50%) and seven with low prevalence (< 10%). Surveys with appropriate sampling procedures aimed either at representative target groups (school-aged children) or communities are recommended for the ZMP and other endemic areas not only to provide reliable information on the current situation of schistosomiasis but also to plan adequate control strategies. 相似文献
2.
Nova MX Borges LR de Sousa AC Brasileiro BT Lima EA da Costa AF de Oliveira NT 《Genetics and molecular research : GMR》2011,10(1):311-320
Onion anthracnose, caused by Colletotrichum gloeosporioides, is one of the main diseases of onions in the State of Pernambuco. We examined the pathogenicity of 15 C. gloeosporioides strains and analyzed their genetic variability using RAPDs and internal transcribed spacers (ITS) of the rDNA region. Ten of the strains were obtained from substrates and hosts other than onion, including chayote (Sechium edule), guava (Psidium guajava), pomegranate (Punica granatum), water from the Capibaribe River, maracock (Passiflora sp), coconut (Cocus nucifera), surinam cherry (Eugenia uniflora), and marine soil; five isolates came from onions collected from four different regions of the State of Pernambuco and one region of the State of Amazonas. Pathogenicity tests were carried out using onion leaves and bulbs. All strains were capable of causing disease in leaves, causing a variable degree of lesions on the leaves; four strains caused the most severe damage. In the onion bulb tests, only three of the above strains caused lesions. Seven primers of arbitrary sequences were used in the RAPD analysis, generating polymorphic bands that allowed the separation of the strains into three distinct groups. The amplification products generated with the primers ITS1 and ITS4 also showed polymorphism when digested with three restriction enzymes, DraI, HaeIII and MspI. Only the latter two demonstrated genetic variations among the strains. These two types of molecular markers were able to differentiate the strain from the State of Amazonas from those of the State of Pernambuco. However, there was no relationship between groups of strains, based on molecular markers, and degree of pathogenicity for onion leaves and bulbs. 相似文献
3.
Samico Fernandes EF Samico Fernandes MF Kim PC de Albuquerque PP de Souza Neto OL de S Santos A de Moraes ÉP de Morais EG Mota RA 《The Journal of parasitology》2012,98(3):690-691
The object of this study was to investigate Toxoplasma gondii antibodies and parasite DNA in pigs in the state of Pernambuco, Brazil. Blood samples were collected from 305 slaughtered pigs in 11 municipalities, and their sera were tested for T. gondii antibodies using the indirect fluorescent antibody test (IFAT, cutoff 1:64); 38 (12.5%) samples were positive. Attempts were made to detect T. gondii DNA in the heart tissue of seropositive pigs using the B 1 gene and PCR; 21 (55.2%) of the 38 hearts were positive. This is the first detection of T. gondii DNA in tissues of serologically positive swine in the State of Pernambuco, Brazil. 相似文献
4.
Floristic similarities between lowland and montane areas of Atlantic Coastal Forest in Northeastern Brazil 总被引:1,自引:1,他引:0
Ferraz Elba Maria Nogueira Araújo Elcida de Lima da Silva Suzene Izídio 《Plant Ecology》2004,174(1):59-70
Studies undertaken in the Atlantic Coastal Forest have revealed a notable floristic heterogeneity within this vegetation type
in NE Brazil. However, there is still a great need for detailed comparisons of the floristic relationships between the various
forest types found there. This work presents an analysis of the floristic similarity (at the species level) of these forest
as revealed by floristic surveys, with the aim of better understanding and defining this vegetation. Using a binary matrix,
grouping, ordering, and TWINSPAN analysis were performed on 742 tree/shrub species listed in 35 different plant surveys. These
tree/shrub species were divided by these analysis into two large floristic groups – ombrophilous and semideciduous. The semideciduous
group was formed, in general, by forest areas located at altitudes above 700 m (montane forests), and could itself be divided
into two subgroups. The first subgroup was located more inland (Pernambuco), while the second subgroup was located nearer
the coast (within the states of Pernambuco and Ceará). The ombrophilous group was quite heterogeneous, but could also be divided
into two floristic subgroups: i) lowland forests (below 100 m a.s.l) in the states of Pernambuco, Paraíba, and in two areas
of Bahia, as well as some montane forests (in Pernambuco, between 640 and 900 m a.s.l.); ii) lowlands forests in the states
of Alagoas and Bahia. This latter subgroup is the largest and best defined, and has the highest degree of internal similarity.
Nonetheless, it can be further subdivided into two smaller classes, one in Alagoas and the other in Bahia State. The results
of this study demonstrate that the concept of the Atlantic Coastal Forest could also include montane ombrophilous forests
in Pernambuco State, as these forests form a single floristic unit together with lowland forests in that state.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
5.
A new species of Acritopappus, A. buiquensis, is described and illustrated from Pernambuco State, Brazil. This is the second record for the genus in the State. The position
of A. buiquensis in the genus is discussed. 相似文献
6.
We studied the mycorrhizal condition of three subclasses (Alismatidae, Arecidae, Zingiberidae) of Liliopsida (Monocotyledonae)
occurring in different natural areas of the State of Pernambuco, Northeast Brazil. Twenty-two of 35 specimens (62.8%) were
colonized by arbuscular mycorrhizal (AM) fungi. No association was found in specimens of five families (Alismataceae, Cannaceae,
Cyclanthaceae, Hydrocharitaceae, Limnocharitaceae), but in two other families (Costaceae, Zingiberaceae) all specimens formed
mycorrhizae. Four families showed variable mycotrophy (Araceae, Bromeliaceae, Heliconiaceae, Marantaceae). This is the first
evaluation of the mycorrhizal condition of 24 Monocotyledonae species from this region, 15 of which were found to form AM.
Accepted: 4 July 2000 相似文献
7.
S R Lacerda M L Koening S Neumann-Leit?o M J Flores-Montes 《Revista brasleira de biologia》2004,64(1):81-94
The Botafogo estuary is of socio-economical importance for Pernambuco State. It is located at the north of Santa Cruz Channel, Itamaracá, Pernambuco, Brazil (07 degrees 42' 50 "S and 34 degrees 52' 10" W). There is a critical need to understand its functioning because of the rate at which this area is being converted to land uses. The phytoplankton dynamics was studied to enhance the knowledge of and verify the possible changes which have occurred in this ecosystem. Sampling was carried out with a plankton net 65 micrometers mesh size for qualitative data, and a Van Dorn bottle for quantitative data. Concurrent hydrological and chlorophyll-a data were collected. Samplings were made in one fixed station in July 1996 (rainy season) and December 1996 (dry season), at 3-hour intervals during 24 hours. Eighty-seven specific and infra-specific taxa were identified from net plankton samples. Diatoms were most frequent, mainly Coscinodiscus centralis and Odontella regia in the rainy and dry seasons, respectively. Phytoplankton density varied from 205,000 to 1,210,000 cell.L-1 in the dry season, and from 230,000 to 2,510,000 cell.L-1 in the rainy season, indicating eutrophic conditions. Most numerically abundant were the diatoms Cyclotella meneghiniana and Cylindrotheca closterium and the phytoflagellates. The ecosystem is polluted, and deleterious effects are minimized by the marine influence which allows periodic water renewal. 相似文献
8.
《Journal of bryology》2013,35(4):291-294
AbstractThe seasonality of maturation of gametangia and development of sporophytes of the autoicous moss Octoblepharum albidum Hedw. was investigated in a remnant of the Atlantic Forest in Pernambuco State, Brazil. Two populations of this species were sampled monthly for a period of 2 years. The results obtained were similar in both populations and are presented together. The average number of antheridia produced per perigonium was 13.4 and of archegonia per perichaetium was 6.7. The frequency of juvenile, immature and mature gametangia was considerably greater during the rainy season, when there was also an increase in fertilized archegonia. Sporophytes began their development during the rainy season and continued into the dry season, when the spores were dispersed. The reproductive pattern of this species presented a marked seasonality,apparently greatly influenced by rainfall. 相似文献
9.
C R de Almeida A M de Almeida D P Brasil J Dantas Sobrinho M A Leal 《Memórias do Instituto Oswaldo Cruz》1986,81(4):409-416
The occurrence of the rodent Akodon arviculoides Wagner, 1842 in the plague focus of the "Agreste" region of the State of Pernambuco and a report on its ability for survival, reproduction and development in captivity, its susceptibility to Yersinia pestis infection and the role of this rodent species in Brazilian plague foci are reported. 相似文献
10.
Males of Zophobas aff. confusus and Nyctobates gigas (Tenebrionidae) collected in the State of Pernambuco, Brazil, were studied through conventional staining, C-banding, silver nitrate impregnation (AgNO(3)), and the base specific fluorochromes CMA(3) and DAPI. Z. aff. confusus was found to have 2n = 20 (9+Xyp) while N. gigas exhibited 2n = 18 (8+neoXY). Large pericentromeric blocks of constitutive heterochromatin (CH) were detected throughout the autosomal complement of the two species, except in one autosomal pair of N. gigas in which no heterochromatic block was observed. The sex chromosomes of both species were almost totally heterochromatic. Double staining with CMA(3)/DA (distamycin) and DAPI/DA marked CH in Z. aff. confusus. However, DAPI staining was more intense. N. gigas was found to possess blocks of CH-positive CMA(3) and homogeneous DAPI. AgNO(3) staining also revealed differences between the two species. In Z. confusus an NOR was observed in the sexual bivalent Xyp and N. gigas was found to have an autosomal NOR. 相似文献
11.
12.
Loss of genetic diversity in farmed populations of Colossoma macropomum estimated by microsatellites
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C. H. A. Santos G. X. Santana C. S. Sá Leitão M. N. Paula‐Silva V. M. F. Almeida‐Val 《Animal genetics》2016,47(3):373-376
The genetic variability of four Colossoma macropomum broodstocks, three from fish farms in different regions and one from the natural environment in Brazil, was analyzed using microsatellite markers. The wild progeny (n = 30) were caught in the Solimões–Amazonas River, at the varzea lakes; this location is used to mature the fish from larvae to juveniles. The three fish farms were selected according to the age of their lineages and broodstock availability: DNOCS (n = 21) is located in the Ceará State, representing the oldest lineage of cultivated tambaqui in Brazil; Balbina (n = 30) is located in the Amazonas State, representing the youngest stocks of tambaqui farmed in Brazil (approximately 15 years); and UFRPE (n = 30) is located in the Pernambuco State and is considered to be a mixed stock formed from the DNOCS and Balbina lineages. The analysis of 13 microsatellite loci indicated the occurrence of a variability reduction in the farmed populations; the UFRPE stock was the population with the highest diversity level. Low values of molecular coancestry were found in these populations. Additionally, significant differences in the RST values among the populations were detected, as was the occurrence of genetic structure. The genetic loss found in these populations may have been influenced by the founder effect. Because no breeding programs were during the entire production period and no pedigree records were kept for these broodstocks, we suggest that a wild population might be used as an important genetic resource to increase the genetic diversity of renewal stock lineages. 相似文献
13.
Ariadna Valentina Lopes Isabel Cristina Machado 《Plant Systematics and Evolution》1998,213(1-2):71-90
Between March 1993 and May 1995 the floral biology and the phenology ofClusia nemorosa
G. Mey. (Clusiaceae) were studied in two dioecious populations in Pernambuco State, and some observations were made in gynodioecious populations, that were encountered only in Bahia State. The species flowers from June to January/February. The flowers are bowl shaped, and last only one day. The floral reward is resin which is produced by staminodia in all types of flowers. The sex ratio was 1:1 for the studied populations. The pollen viability was approximately 98%. Apomixis was not observed. The rate of fruit set, in hermaphrodite individuals, under natural conditions and the sex ratio of the gynodioecious populations studied suggest that the hermaphrodite individuals may behave as males.Clusia nemorosa is melittophilous and was visited by 16 species of bees. The main pollinators wereEuplusia mussitans, E. iopyhrra, Eulaema cingulata, E. nigrita, Euglossa cordata andEuglossa sp. 相似文献
14.
Maria Dulcinéia Sales dos Santos Tiago Jord?o Porto Rejane Maria Lira-da-Silva Tania Kobler Brazil 《ZooKeys》2014,(435):49-61
The male of Tityus kuryi Lourenço, 1997 is described for the first time. Despite being very similar to the female, the male presents more robust metasomal segments. Additionally, the distribution of the sexual populations of another two species of the T. stigmurus complex is reported herein: T. serrulatus Lutz & Mello, 1922 and T. stigmurus (Thorell, 1877). Males of T. serrulatus were, until now, restricted to the Minas Gerais State (Southwestern region of Brazil), and with new records reported here, its known distribution now encompasses the Northeastern region of Brazil. Males of T. stigmurus were previously recorded only for two municipalities in the State of Bahia, and here we present eight new records for Bahia State and one for Pernambuco State. We present a key to related species of the T. stigmurus complex based on morphology and coloration pattern. 相似文献
15.
By R. P. Lessa A. Monteiro P. J. Duarte-Neto A. C. Vieira 《Zeitschrift fur angewandte Ichthyologie》2009,25(3):256-268
A total of 17 fishery systems covering gillnets, traps and seines targeting fish and crustaceans as well as hand-collected mussels in the state of Pernambuco (Brazil) were compared and analyzed in the present study using the RAPFISH method and 57 attributes to qualify five evaluation dimensions: economic, social, ecological, technological and management. The aim was to determine the sustainability of each field from the fishery (i.e. stocks) and social standpoint (i.e. fishermen). With regard to sustainability, it was generally apparent that the fisheries analyzed are far from any ideal that would permit long-term exploitation, but are nonetheless also distant from the extremes of non-sustainability in the environments investigated. The low degree of organization demonstrated in most fisheries of Pernambuco and the low level of schooling among the fishermen contribute toward maintaining the status quo, with an increase in situations of conflict and a lack of valorization regarding the activities. The shrimp system is the least sustainable, mainly due to its environmental impact; however, this is compensated by the relatively higher quality of living provided stakeholders through its exploitation. The evaluation dimensions showed the most sustainable system in Pernambuco to be the stationary 'uncovered pound net', followed by the line system. Alternative procedures for integrated fishery management, such as an increase in statistical data, coastal zoning to limit shrimp farms and establishing protected areas are proposed and discussed. Such procedures may contribute toward the formulation of public policies for the fishery industry of the state, which is essentially made up of artisanal fisheries with low yields and exercised by the 11 926 fishermen affiliated with coastal fishing colonies. 相似文献
16.
N C Leal T I Cavalcanti M Jesuita B Da Silva E M dos Reis C A Solari E Hofer 《Memórias do Instituto Oswaldo Cruz》1988,83(4):475-479
326 samples of diarrheal feces obtained from children whose ages ranged from zero to 5 years, admitted in two rehydration hospitals in the city of Recife, Pernambuco, were analyzed. Feces were placed in Cary-Blair medium (4 degrees C) for shipment to the laboratory. There was no difference in the rate of bacteria isolation if the samples were analyzed within the period from 3 to 7 days of collection. 19.02% of the analyzed samples were positives for at least one of the searched bacteria, 26 Salmonella belonging to 3 species, 21 classic enteropathogenic E. coli, 1 invasive E. coli, 10 Shigella belonging to 3 serotypes and 1 Yersinia enterocolitica were found. 相似文献
17.
Laguna MM Amaro RC Mott T Yonenaga-Yassuda Y Rodrigues MT 《Genetics and molecular biology》2010,33(1):57-61
The karyotype of Amphisbaena ridleyi, an endemic species of the archipelago of Fernando de Noronha, in State of Pernambuco, Brazil, is described after conventional staining, Ag-NOR impregnation and fluorescence in situ hybridization (FISH) with a telomeric probe. The diploid number is 46, with nine pairs of macrochromosomes (three metacentrics, four subtelocentrics and two acrocentrics) and 14 pairs of microchromosomes. The Ag-NOR is located in the telomeric region of the long arm of metacentric chromosome 2 and FISH revealed signals only in the telomeric region of all chromosomes. Further cytogenetic data on other amphisbaenians as well as a robust phylogenetic hypothesis of this clade is needed in order to understand the evolutionary changes on amphisbaenian karyotypes. 相似文献
18.
Oliveira JB Madruga CR Schenk MA Kessler RH Miguita M Araújo FR 《Memórias do Instituto Oswaldo Cruz》2003,98(3):395-400
Antigenic characterization of Anaplasma marginale isolates, by identifying conserved and variable epitopes of major surface proteins (MSP), is an important tool for vaccine development against this rickettsia. The B cell epitopes of A. marginale isolates from three microregions of the State of Pernambuco and one from the State of Mato Grosso do Sul, Brazil, were characterized by indirect fluorescent antibody technique (IFAT) and Western blot (WB) with 15 monoclonal antibodies (MAbs). The epitope recognized by MAb ANA22B1 (MSP-1a) was conserved by IFAT and WB (73-81 kDa). MSP-2 epitopes recognized by MAbs ANAO58A2 and ANAO70A2 were conserved by IFAT, while ANAO50A2 and ANA66A2 epitopes were polymorphic; in the WB, the MAbs ANAO50A2 and ANAO70A2 identified bands of 45 kDa only in the Pernambuco-Mata isolate. None of the isolates reacted with MAb ANAR75C2 (MSP-3). The MSP-4 epitope recognized by MAb ANAR76A1 was conserved by IFAT, as well as the MSP-5 epitope recognized by MAb ANAF16C1 by IFAT and WB (16 kDa). The MAbs ANAR17A6, ANAR83B3, ANAR94C1, ANAO24D5 and ANAR19A6 identified conserved epitopes by IFAT. MSP-1, MSP-2 and MSP-4, which previously showed partial protection in experimental trials, are also potential immunogens to be employed in Brazil, due to the B cell epitope conservation. 相似文献
19.
EM Dorneles JA Santana GI Andrade EL Santos AS Guimarães RA Mota AS Santos A Miyoshi V Azevedo AM Gouveia AP Lage MB Heinemann 《Genetics and molecular research : GMR》2012,11(3):2051-2059
Corynebacterium pseudotuberculosis, the infectious agent of caseous lymphadenitis (CLA), is responsible for substantial economic losses in goat and sheep production. Molecular characterization of C. pseudotuberculosis isolates by enterobacterial repetitive intergenic consensus (ERIC)-PCR has shown promising results in genotyping strains isolated from sheep with CLA. We evaluated the genetic diversity of C. pseudotuberculosis isolates collected from the Sert?o region of the Pernambuco (PE) State, Brazil, and investigated the potential of ERIC-PCR as a tool for the molecular typing of strains of C. pseudotuberculosis isolated from goats. Thirty-two C. pseudotuberculosis strains isolated from goats in the municipalities of Floresta and Ibimirim, PE, C. pseudotuberculosis type strain ATCC 19410, the 1002 vaccine strain, and a field isolate of Rhodococcus equi were fingerprinted using the primers ERIC-1R and ERIC-2 and the primer pair ERIC- 1R+ERIC-2. Using 100% similarity as the cutoff, 8, 10, and 7 genotypes were obtained with ERIC-1-PCR, ERIC-2-PCR, and ERIC-1+2-PCR, respectively. The Hunter-Gaston discriminatory index calculated for the ERIC-1-PCR was 0.75. The index for the ERIC-2-PCR was 0.88, and the index for the ERIC-1+2-PCR was 0.79. Among goat isolates of C. pseudotuberculosis, three, two and four genotypes (found by ERIC-1-PCR, ERIC-2-PCR, and ERIC-1+2-PCR, respectively) had been previously described among sheep isolates from Minas Gerais State, Brazil. These results showed that ERIC-PCR has good discriminatory power and typeability, making it a useful tool for discrimination among C. pseudotuberculosis isolates from goats. 相似文献
20.
The São Raimundo Nonato (Piauí, Northeastern Brazil) area yielded about 50 Macraucheniidae remains. The study of this material and of other fossils from Northeastern Brazil (Paraíba, Pernambuco, Rio Grande do Norte) shows a great similarity to Macrauchenia patachonica as it was defined in Argentina, which is the most abundant of the three species pertaining to the genus. Thus the taxon Xenorhinotherium bahiense created in 1988 by Cartelle et Lessa for a material discovered in the Bahia State and assigned to use for all the Quaternary Litopterns from tropical Brazil appears to be no more than a junior synonym of it. M. patachonica seems to have occupied very varied biotopes from sea level up to a high of 4000 m, and from the cold Southern Patagonia to the Equatorial Venezuela. The species lived from the Middle Pleistocene up to the beginning of the Holocene. 相似文献