共查询到20条相似文献,搜索用时 15 毫秒
1.
Ryung Yang Akihiro Okitani Masao Fujimaki 《Bioscience, biotechnology, and biochemistry》2013,77(12):2087-2095
The extent of activation of myofibrillar ATPase activity by trypsin treatment has been measured.When myofibril (5 mg/ml) was treated with a low concentration of trypsin (2.5 μg/ml), the Mg-modified ATPase activity of myofibrils at a low ionic strength increased appreciably, while the EDTA-enhanced ATPase activity of myofibrils at a high ionic strength did not change with the progress of trypsin digestion.The dependence of myofibrillar ATPase activity on KCl concentration also became greater with the progress of trypsin digestion.Trypsin treatment caused 5-fold increase in the Mg-modified ATPase activity of 0-myofibril, when treated with trypsin in a ratio of 1 to 2000 myofibril for 80 min. Under the same condition, the ATPase activity of 1-myofibril increased by about 150%, whereas that of 8-myofibril increased by approximately 50%.When myofibrils were treated with trypsin in a ratio of 1 to 200 myofibril, the Mg-ATPase activity of 8-myofibril decreased earlier than that of 1-myofibril did by about 20 min.Experimental results obtained in this study were enough to confirm that the myofibrils from the aged muscle are more susceptible to tryptic action.An assumption was made that the structural alteration of myofibrils during aging might be attributed to the change in thin filament of myofibrils, including Z-lines, which are mainly due to the change in the native tropomyosin of thin filaments. 相似文献
2.
The ability of CASF (Ca2+-activated sarcoplasmic factor), a proteolytic enzyme that has recently been isolated from muscle and that removes Z-disks from myofibrils, to remove soluble material from myofibrils and to alter the Mg2+-modified ATPase activity of myofibrils was studied. A new assay involving determination of soluble material released from myofibrils was developed to measure CASF activity quantitatively. Optimum pH and optimum Ca2+ concentration for CASF activity as determined by this new assay were 7.0 and 1 mm, respectively. Proteolytic activity of CASF on myofibrils was prevented completely by excess EDTA. CASF treatment of myofibrils at CASF to myofibril ratios of 1: 20 by weight for 30 min caused a 20~25% increase in Mg2+-modified ATPase activity. CASF treatment for 360 min under these same conditions caused a decrease in Mg2+-modified ATPase activity at the highest ionic strengths used in this study (46.7 and 66.7 mm KCI). The increase in Mg2+-modified ATPase activity may originate from CASF degradation of troponin, whereas the decrease in Mg2+- modified ATPase activity may be due to CASF destruction or release of α-actinin from myofibrils. Digestion of myofibrils by CASF causes in the myofibrils (degradation of Z-lines, increase of ATPase activity) that are very similar to the changes caused by postmortem storage. 相似文献
3.
Nobuhiko Arakawa Akihiro Okitani Masao Fujimaki 《Bioscience, biotechnology, and biochemistry》2013,77(6):574-580
The chromatography on Cellex D DEAE-SF (Bio-Rad Lab.) or TEAE-cellulose (Serva) equilibrated against 0.28 m KCl solution containing 0.02 m tris-HCl buffer at pH 8.0 was found to be suitable for the refinement of myosin B.The ultraviolet absorption spectrum and ATPase activity of the eluted fractions showed that “myosin B” was fractionated and purified by this technique, especially by the preferential removal of the fraction suggested as ribonucleic acid related substance.The chromatography may provide the effective way to investigate changes of “myosin B” during aging of meat. 相似文献
4.
Yuji Koide Mamoru Honma Tokuji Shimomura 《Bioscience, biotechnology, and biochemistry》2013,77(7):1171-1177
Branched chain amino acid aminotransferase was partially purified from Pseudomonas sp. by ammonium sulfate fractionation, aminohexyl-agarose and Bio-Gel A-0.5 m column chromatography.This enzyme showed different substrate specificity from those of other origins, namely lower reactivity for l-isoleucine and higher reactivity for l-methionine.Km values at pH 8.0 were calculated to be 0.3 mm for l-leucine, 0.3 mm for α-ketoglutarate, 1.1 mm for α-ketoisocaproate and 3.2 mm for l-glutamate.This enzyme was activated with β-mercaptoethanol, and this activated enzyme had different kinetic properties from unactivated enzyme, namely, Km values at pH 8.0 were calculated to be 1.2 mm for l-leucine, 0.3 mm for α-ketoglutarate.Isocaproic acid which is the substrate analog of l-leucine was competitive inhibitor for pyridoxal form of unactivated and activated enzymes, and inhibitor constants were estimated to be 6 mm and 14 mm, respectively. 相似文献
5.
d-Arabinose(l-fucose) isomerase (d-arabinose ketol-isomerase, EC 5.3.1.3) was purified from the extracts of d-arabinose-grown cells of Aerobacter aerogenes, strain M-7 by the procedure of repeated fractional precipitation with polyethylene glycol 6000 and isolating the crystalline state. The crystalline enzyme was homogeneous in ultracentrifugal analysis and polyacrylamide gel electrophoresis. Sedimentation constant obtained was 15.4s and the molecular weight was estimated as being approximately 2.5 × 105 by gel filtration on Sephadex G-200.Optimum pH for isomerization of d-arabinose and of l-fucose was identical at pH 9.3, and the Michaelis constants were 51 mm for l-fucose and 160 mm for d-arabinose. Both of these activities decreased at the same rate with thermal inactivation at 45 and 50°C. All four pentitols inhibited two pentose isomerase activities competitively with same Ki values: 1.3–1.5 mm for d-arabitol, 2.2–2.7 mm for ribitol, 2.9–3.2 mm for l-arabitol, and 10–10.5 mm for xylitol. It is confirmed that the single enzyme is responsible for the isomerization of d-arabinose and l-fucose. 相似文献
6.
Hirokazu Matsui Seiya Chiba Tokuji Shimomura 《Bioscience, biotechnology, and biochemistry》2013,77(10):1855-1860
The substrate specificity of sugar beet α-giucosidase was investigated. The enzyme showed a relatively wide specificity upon various substrates, having α-1,2-, α-1,3-, α-1,4- and α-l,6-glucosidic linkages.The relative hydrolysis velocity for maltose (G2), nigerose (N), kojibiose (K), isomaltose (I), panose (P), phenyl-a-maltoside (?M) and soluble starch (SS) was estimated to be 100:130: 10.7: 22.6: 54.6: 55.8: 120 in this order; that for malto-triose (G3), -tetraose (G4), -pentaose (G5), -hexaose (G6), -heptaose (G7), -octaose (G8), amyloses (G13) and (G17), 91: 91: 91: 91: 80: 57: 75: 73. The Km values for N, K, I, P, and SS were 16.7 mM, 1.25 mM, 10.8 mM, 8.00 mM, 4.12 mM and 1.90 mg/ml, respectively; that for G2, G3, G4, G5, G6, G7, G8, G13 and G17 were 20.0 mM, 3.67 mM, 2.34 mM, 0,64 mM, 0.42 mM, 0.32 mM, 0.23 mM, 0.36 mM and 0.26 mM, respectively.The enzyme, though showed higher affinity and activity toward soluble starch than toward maltose, was considered essentially to be an α-glucosidase. 相似文献
7.
Yuichi Hatsuda Takashi Hamasaki Masayoshi Ishida Kuniaki Matsui Shinichiro Hara 《Bioscience, biotechnology, and biochemistry》2013,77(3):521-522
L-Arabinose isomerase (L-arabinose ketol-isomerase, EC 5.3.1.4) was demonstrated from the L-arabinose-grown cells of Streptomyces sp. which was isolated from sea water. The enzyme was purified by MnCl2 treatment, fractionation by polyethylene glycol and by column chromatographies on Sephadex G-150 and DEAE-cellulose. The purified enzyme was specific only for L-arabinose and the Michaelis constant for L-arabinose was 40 mM at pH 7.5. Manganese or cobalt ions were effective for the enzyme activity after dialysis against EDTA. The enzyme activity was inhibited competitively by L-arabitoI, ribitol and xylitol, of which inhibition constants were 1.1, 1.0, and 15 mM, respectively. 相似文献
8.
Production of d-xylose and l-arabinose isomerases by lactic acid bacteria was greatly promoted by the addition of manganese ions in cultural medium. Effective concentration of the ions was 5 × 1O-3 m. Ferrous ions were also effective for the production of d-xylose isomerase and cobaltous ions were somewhat effective for the production of l-arabinose isomerase. Zinc and cadmium ions inhibited bacterial growth. It was possible to increase the production of isomerase by changing MnSO4 concentration to 5× 10-3 m (0.l1 %) in place of 0.001 per cent in the normal medium.Column chromatographic procedures for the purification of pentose isomerases were carried out. Cation and anion exchange resins were not suitable because of their low exchange capacities and instability of the enzyme at acidic pH range. But the isomerases were successfully purified by DEAE-cellulose column chromatography with high recovery (85~90%). Using a Tris buffer, KCl concentration was increased in gradient. d-Xylose isomerase was eluted at pH 7.0 at 0~0.2 m KCl, and l-arabinose isomerase at pH 8.0 at 0~0.4 m KCl. The purified isomerases, d-xylose isomerase and l-arabinose isomerase, both required manganese ions specifically for their activities.D-Xylose isomerase and l-arabinose isomerase are different enzymes which can be separated from each other with acetone fractionation at pH 4.8~5.0, heat treatment or chromatography on a colnmn of DEAE-cellulose. In DEAE-cellulose chromatography with a linear gradient elution method, d-xylose isomerase is recovered in the first peak at pH 7.0 (Tris bnffer) with 0~0.2 m KCl, and l-arabinose isomerase is eluted in the second peak at pH 8.0 (Tris buffer) with a larger ionic strength. 相似文献
9.
Regulatory properties of the enzymes in l-tyrosine and l-phenyalanine terminal pathway in Corynebacterium glutamicum were investigated. Prephenate dehydrogenase was partially feedback inhibited by l-tyrosine. Prephenate dehydratase was strongly inhibited by l-phenylalanine and l-tryptophan and 100% inhibition was attained at the concentrations of 5 × 10?2mm and 10?1mm, respectively. l-Tyrosine stimulated prephenate dehydratase activity (6-fold stimulation at 1 mm) and restored the enzyme activity inhibited by l-phenylalanine or l-tryptophan. These regulations seem to give the balanced synthesis of l-tyrosine and l-phenyl-alanine. Prephenate dehydratase from C. glutamicum was stimulated by l-methionine and l-leucine similarly to the enzyme in Bacillus subtilis and moreover by l-isoleucine and l-histidine. C. glutamicum mutant No. 66, an l-phenylalanine producer resistant to p-fluorophenyl-alanine, had a prephenate dehydratase completely resistant to the inhibition by l-phenylalanine and l-tryptophan. 相似文献
10.
l-Fucose (l-galactose) dehydrogenase was isolated to homogeneity from a cell-free extract of Pseudomonas sp. No 1143 and purified about 380-fold with a yield of 23 %. The purification procedures were: treatment with polyethyleneimine, ammonium sulfate fractionation, chromatographies on phenyl-Sepharose and DEAE-Sephadex, preparative polyacrylamide gel electrophoresis, and gel filtration on Sephadex G-100. The enzyme had a molecular weight of about 34,000. The optimum pH was at 9 — 10.5 and the isoelectric point was at pH 5.1. l-Fucose and l-galactose were effective substrates for the enzyme reaction, but d-arabinose was not so much. The anomeric requirement of the enzyme to l-fucose was the β-pyranose form, and the reaction product from l-fucose was l-fucono- lactone. The hydrogen acceptor for the enzyme reaction wasNADP+, and NAD + could be substituted for it to a very small degree. Km values were 1.9mm, 19mm, 0.016mm, and 5.6mm for l-fucose, l- galactose, NADP+, and NAD+, respectively. The enzyme activity was strongly inhibited by Hg2 +, Cd2 +, and PCMB, but metal-chelating reagents had almost no effect. In a preliminary experiment, it was indicated that the enzyme may be usable for the measurement of l-fucose. 相似文献
11.
A bacterial strain, HN-500, having an activity of d-glucose isomerization was newly isolated from soil, and was identified to be similar to Escherichia intermedia (Werkman and Gillen) Vaughn and Levine. The strain, grown on wide varieties of carbon sources, shows definitely d-glucose isomerizing activity in the presence of arsenate. d-Fructose formed in reaction mixture was identified by paper chromatography and was isolated in crystalline form from calcium-fructose complex. In order to increase the production of d-glucose isomerase, d-glucose and ammonium nitrogen were effective carbon and nitrogen sources, respectively, but none of the metallic ions tested were effective, furthermore manganese, ferrous and ferric ions present mOre than 10-5m in growth medium fully repressed the enzyme formation. The cells grown on carbon sources other than d-xylose showed no activity of d-xylose isomerase. 相似文献
12.
The crystalline d-mannitol dehyrogenase (d-mannitol:NAD oxidoreductase, EC 1.1.1.67) catalyzed the reversible reduction of d-fructose to d-mannitol. d-Sorbitol was oxidized only at the rate of 4% of the activity for d-mannitol. The enzyme was inactive for all of four pentitols and their corresponding 2-ketopentoses. The apparent optimal pH for the reduction of d-fructose or the oxidation of d-mannitol was 5.35 or 8.6, respectively. The Michaelis constants were 0.035 m for d-fructose and 0.020 m for d-mannitol. The enzyme was also found to be specific for NAD. The Michaelis constans were 1 × 10?5 m for NADH2 and 2.7 × 10?4 m for NAD. 相似文献
13.
Kazuo Yonaha Seizen Toyama Masaaki Yasuda Kenji Soda 《Bioscience, biotechnology, and biochemistry》2013,77(9):1701-1706
ω-Amino acid: pyruvate aminotransferase, purified to homogeneity and crystallized from a Pseudomonas sp. F–126, has a molecular weight of 172,000 or 167,000±3000 as determined by the gel-filtration or sedimentation equilibrium method, respectively. The enzyme catalyzes the transamination between various ω-amino acids or amines and pyruvate which is the exclusive amino acceptor. α-Amino acids except l-α-alanine are inert as amino donor. The Michaelis constants are 3.3 mm for β-alanine, 19 mm for 2-aminoethane sulfonate and 3.3 mm for pyruvate. The enzyme has a maximum activity in the pH range of 8.5~10.5. The enzyme is stable at pH 8.0~10.0 and at up to 65°C at pH 8.0. Carbonyl reagents strongly inhibit the enzyme activity. Pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate reactivate the enzyme inactivated by carbonyl reagents. The inhibition constants were determined to be 0.73 mm for d-penicillamine and 0.58 mm for d-cycloserine. Thiol reagents, chelating agents and l-α-amino acids showed no effect on the enzyme activity. 相似文献
14.
《Bioscience, biotechnology, and biochemistry》2013,77(10):1730-1735
L-Tartrate in wines and grapes was enzymatically quantified by using the secondary activity of D-malate dehydrogenase (D-MDH). NADH formed by the D-MDH reaction was monitored spectrophotometrically. Under the optimal conditions, L-tartrate (a 1.0 mM sample solution) was fully oxidized by D-MDH in 30 min. A linear relationship was obtained between the absorbance difference and the L-tartrate concentration in the range of a 0.02-1.0 mM sample solution with a correlation coefficient of 0.9991. The relative standard deviation from ten measurements was 1.71% at the 1.0 mM sample solution level. The proposed method was compared with HPLC, and the values determined by both methods were in good agreement. 相似文献
15.
Chieko Kitada Masahiko Fujino Youji Sakagami Mitsuru Yoshida Akira Isogai Akinori Suzuki 《Bioscience, biotechnology, and biochemistry》2013,77(4):1049-1051
Myosin and reconstituted actomyosin free of regulatory proteins were mixed in 0.6 m KCl and 20 mm phosphate buffer at pH 6.0 and were tested quantitatively for thermally induced gelation properties by measuring the rigidity of the system at 65°C. Full enhancement of gelation was attained when the weight ratio of myosin-to-actomyosin was about 4. The addition of regulatory proteins to actomyosin could restore calcium sensitivity of the contractile system, but did not affect the heat-induced gelation of myosin in the presence of actomyosin, suggesting that regulatory proteins play no role in the heat-induced gelation of the system.Neither the single and double headed subfragments, both capable of interacting with F-actin, nor the helical tail subfragments, devoid of the intracting site with F-actin, exhibited changes in thermogelling properties when mixed with F-actin. However, upon addition to F-actomyosin, the tail subfragments revealed a significant effect on the gelation of actomyosin, whereas the headed subfragments exerted no influence over gelation of the system. These results indicate that the enhancing effect of F-actin on the heat-induced gelation of myosin was brought about solely by the limited amount of F-actomyosin formed in the system, which acts as a cross-linker between the tail portion of bound and free myosin molecules. 相似文献
16.
Nobuo Nakamura Masako Fujita Kazuo Kimura 《Bioscience, biotechnology, and biochemistry》2013,77(12):2827-2833
l-Arginase (l-arginine amidinohydrolase, EC 3.5.3.1) was purified in a crystalline form from cells of Bacillus subtilis KY 3281 with an overall yield of 23.2%. The crystalline enzyme had a specific activity of 858 i.u./mg-protein and was ultracentrifugally homogeneous. It was estimated to have a molecular weight of 115,000±5000 by the method of Yphantis.The enzyme highly specific for l-arginine showed the maximum activity at pH 10 with Mn2+ ion. The Km for l-arginine was 1.35 × 10?2 m The activity was competitively inhibited by l-lysine, but not by l-ornithine and increased by the addition of Mn2+ or Co2+ ions. The stable pH and temperature ranges became wider in the presence of Mn2+ ion and l-threonine. 相似文献
17.
《Bioscience, biotechnology, and biochemistry》2013,77(8):1652-1655
D-Galactosyl-β1→4-L-rhamnose (GalRha) was produced enzymatically from 1.1 M sucrose and 1.0 M L-rhamnose by the concomitant actions of four enzymes (sucrose phosphorylase, UDP-glucose-hexose 1-phosphate uridylyltransferase, UDP-glucose 4-epimerase, and D-galactosyl-β1→4-L-rhamnose phosphorylase) in the presence of 1.0 mM UDP-glucose and 30 mM inorganic phosphate. The accumulation of GalRha in 1 liter of the reaction mixture reached 230 g (the reaction yield was 71% from L-rhamnose). Sucrose and fructose in the reaction mixture were removed by yeast treatment, but isolation of GalRha by crystallization after yeast treatment was unsuccessful. Finally, 49 g of GalRha was isolated from part of the reaction mixture with yeast treatment by gel-filtration chromatography. 相似文献
18.
Osamu Tosaka Yasuhiko Yoshihara Shigeho Ikeda Koichi Takinami 《Bioscience, biotechnology, and biochemistry》2013,77(5):1305-1312
Better producers of l-lysine were obtained by derivation of fluoropyruvate(FP)-sensitive mutants from Brevibacterium lactofermentum AJ3990. The coexistence of FP and excess biotin synergistically stimulated l-lysine formation by washed cells. FP inhibited 50% of growth and pyruvate dehydrogenase (PDH) activity of AJ3990 at 0.04 mm and 1 mm, respectively. Therefore, the synergistic effect of FP and excess biotin seems to be due to the optimization of the PDH/pyruvate carboxylase activity ratio in l-lysine biosynthesis. This was confirmed by the derivation of FP-sensitive mutants which have the optimal level of PDH activity for l-lysine production. The best producer, AJ11204, had about 27% PDH activity as compared with the parental strain and accumulated 70 g of l-lysine per liter with a conversion yield of 50% from glucose in the presence of excess biotin. 相似文献
19.
Yoshiro Kamata Jun-ichi Kimigafukuro Kazuo Shibasaki 《Bioscience, biotechnology, and biochemistry》2013,77(9):1817-1823
Digestibilities of native, 5 m urea-denatured and 8 m urea-denatured glycinin were studied. Urea was removed by dialysis before digestion. The tryptic digestion of the proteins are influenced by ionic strength. Under low ionic strength condition (0 m NaCl), the proteins, even native glycinin, are well degraded. On the other hand, under high ionic strength condition (0.5 m NaCl), native glycinin resists the tryptic attack and 5 m urea-denatured glycinin is best degraded. The digestibility of 8 m urea-denatured glycinin is lower than that of 5 m urea-denatured one under the condition. The gel filtration and electrophoretic properties show that the digestion intermediate like glycinin-T (the intermediate from native glycinin) is contained in the digestion products. These suggest that the urea-denatured protein contains the almost renatured component after removal of urea. A larger amount of the glycinin-T-like protein was detected at 8 m urea denaturation than at 5 m urea. Therefore, glycinin renatures more readily from 8 m urea denaturation. Probably this is the cause of the decreased digestibility at 8 m urea denaturation. 相似文献
20.
Gen-ichi Danno Sadahiko Yoshimura Masato Natake 《Bioscience, biotechnology, and biochemistry》2013,77(3):284-292
d-Glucose-isomerizing enzyme has been extracted in high yield from d-xylose-grown cells of Bacillus coagulans, strain HN-68, by treating with lysozyme, and purified approximately 60-fold by manganese sulfate treatment, fractionation with ammonium sulfate and chromatography on DEAE-Sephadex column. The purified d-glucose-isomerizing enzyme was homogeneous in polyacrylamide gel electrophoresis and ultracentrifugation and was free from d-glucose-6-phosphate isomerase. Optimum pH and temperature for activity were found to be pH 7.0 and 75°C, respectively. The enzyme required specifically Co++ with suitable concentration for maximal activity being 10?3 m. In the presence of Co++, enzyme activity was inhibited strongly by Cu++, Zn++, Ni++, Mn++ or Ca++. At reaction equilibrium, the ratio of d-fructose to d-glucose was approximately 1.0. The enzyme catalyzed the isomerization of d-glucose, d-xylose and d-ribose. Apparent Michaelis constants for d-glucose and d-xylose were 9×10?2 m and 7.7×10?2 m, respectively. 相似文献