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1.
Streptomyces hygroscopicus No. B–5050-HA, which produces a mixture of six maridomycins, yielded a mutant which produced 75% of the mixture as maridomycin III (MDM III).Growth of S. hygroscopicus No. B–5050-HA, an improved MDM producer, was almost completely inhibited by 20 µg/ml of valine. This inhibition was counteracted by the addition of isoleucine, threonine, homoserine, methionine, α-amino-n-butyrate and α-ketobutyrate.A valine resistant mutant, strain AV was isolated and found to produce increased level of MDM III at the expense of other maridomycins. Production of MDM III by the parent strain depended on the addition of isoleucine to the medium, but that by this mutant did not.The properties of strain AV were discussed. 相似文献
2.
Evidence is presented in this paper to show that synaptosomal particles derived from brains of immature rats possess two separate, high-affinity transport systems for the hydroxyamino acids, serine and threonine. One of these is strictly Na+-dependent and the other Na+-nondependent. The Km terms of both of these systems have values in the range of 20 μM. Na+-dependent uptakes of serine and threonine do not take place in synaptosomal particles isolated from of adult animals. 相似文献
3.
B D Hansen 《Experimental parasitology》1979,48(2):296-304
Trypanosoma gambiense absorbed 14C-labeled lysine, arginine, glutamate, phenylalanine, methionine, threonine, glycine, and alanine by mediated transport systems. The interactions of these compounds as inhibitors or stimulators formed complex patterns of uptake which suggested the presence of five binding and/or transport loci: Locus A bound glutamate, arginine, and lysine, and the binding of glutamate or arginine stimulated the transport of lysine. Locus B transported threonine, glycine, and alanine and appeared to be partially sensitive to ouabain and Na+. Locus C transported glutamate, locus D transported phenylalanine and methionine, and locus E transported lysine and arginine. 相似文献
4.
Joao B. T. Rocha Herman Wolosker †Diogo O. Souza Leopoldo de Meis 《Journal of neurochemistry》1996,66(2):772-778
Abstract: Rat brain microsomes accumulate Ca2+ at the expense of ATP hydrolysis. The rate of transport is not modulated by the monovalent cations K+, Na+, or Li+. Both the Ca2+ uptake and the Ca2+-dependent ATPase activity of microsomes are inhibited by the sulfated polysaccharides heparin, fucosylated chondroitin sulfate, and dextran sulfate. Half-maximal inhibition is observed with sulfated polysaccharide concentrations ranging from 0.5 to 8.0 µg/ml. The inhibition is antagonized by KCl and NaCl but not by LiCl. As a result, Ca2+ transport by the native vesicles, which in the absence of polysaccharides is not modulated by monovalent cations, becomes highly sensitive to these ions. Trifluoperazine has a dual effect on the Ca2+ pump of brain microsomes. At low concentrations (20–80 µM) it stimulates the rate of Ca2+ influx, and at concentrations >100 µM it inhibits both the Ca2+ uptake and the ATPase activity. The activation observed at low trifluoperazine concentrations is specific for the brain Ca2+-ATPase; for the Ca2+-ATPases found in blood platelets and in the sarcoplasmic reticulum of skeletal muscle, trifluoperazine causes only a concentration-dependent inhibition of Ca2+ uptake. Passive Ca2+ efflux from brain microsomes preloaded with Ca2+ is increased by trifluoperazine (50–150 µM), and this effect is potentiated by heparin (10 µg/ml), even in the presence of KCl. It is proposed that the Ca2+-ATPase isoform from brain microsomes is modulated differently by polysaccharides and trifluoperazine when compared with skeletal muscle and platelet isoforms. 相似文献
5.
Methionine sulfoxide is transported into purified intestinal and renal brush border membrane vesicles from rabbit by an Na+-dependent mechanism and is accumulated inside the vesicles against the concentration gradient. Both in intestine and kidney, the rate of transport is enhanced with increasing concentrations of Na+ in the external medium. Increasing the Na+ gradient reduces the apparent Kt for methionine sulfoxide without causing any change in Vmax. With an outward K+ gradient (vesicle > medium), valinomycin stimulates the Na+-gradient-dependent transport of methionine sulfoxide in the kidney, showing the electrogenicity of the transport process. A number of amino acids inhibit methionine sulfoxide transport in both the intestine and kidney. An enzymatic activity capable of reducing methionine sulfoxide to methionine is present in the intestinal mucosa, renal cortex and liver. The activity is highest in renal cortex and lowest in intestine. The methionine sulfoxide-reducing activity is stimulated by NADH, NADPH, glutathione and dithiothreitol and the potency of the stimulation is in the order: dithiothreitol > NADPH > glutathione > NADH. 相似文献
6.
7.
It was shown in previous studies that the giant freshwater alga Chara corallina does not control its Na+‐dependent Pi uptake by monitoring the internal Pi concentration and it was hypothesized that Chara may instead detect changes in Pi supply from the environment. The present work investigated the conditions that control the induction and inactivation of high affinity Na+/Pi influx in Chara. Withdrawal of Pi from the external medium resulted in a gradual increase in the rate of uptake measured immediately after Pi was resupplied. The increase continued for at least 7 d of starvation. In the initial stages, 0·5 or 1 µm Pi were more effective at inducing transport activity than no Pi, suggesting that low levels of Pi are actually required for induction. The high Na+‐dependent Pi uptake observed in Pi‐starved cells was inactivated by treatment with as little as 1 µm Pi over 6 d. External Na+ plays a major role in controlling the capacity for Na+/Pi cotransport activity, and in the absence of Na+, both induction and inactivation were either delayed or abolished. Na+ starvation stimulated Na+ uptake even though there were no measurable changes in the concentrations of Na+, or of K+ or Pi in either the vacuole or cytoplasm. It was concluded that both substrate (Pi) and driver ion (Na+) are required at adequate concentrations for the induction of the cotransporter. In the case of Pi, it was suggested that passive leakage of Pi from the cell into the apoplast is sufficient for this purpose but that supplementation by up to 1 µm Pi is more effective at the earlier stage. A mechanism for sensing the external supply of Pi is proposed. 相似文献
8.
Two filamentous, nitrogen fixing cyanobacteria were examined for their salt tolerance and sodium (Na+) transport.Anabaena torulosa, a saline form, grew efficiently and fixed nitrogen even at 150 mM salt (NaCl) concentration while,Anabaena L-31, a fresh water cyanobacterium, failed to grow beyond 35 mM NaCl.Anabaena torulosa showed a rapidly saturating kinetics of Na+ transport with a high affinity for Na+
(K
m, 0.3 mM).Anabaena L-31 had a much lower affinity for Na+
(Km, 2.8 mM) thanAnabaena torulosa and the pattern of uptake was somewhat different. BothAnabaena spp. exhibited an active Na+ extrusion which seems to be mediated by a Na+-K+ ATPase and aided by oxidative phosphorylation.Anabaena L-31 was found to retain much more intracellular Na+ thanAnabaena torulosa. The results suggest that the saline form tolerates high Na+ concentrations by curtailing its influx and also by an efficient Na+ extrusion, although these alone may not entirely account for its success in saline environment. 相似文献
9.
为提高香蕉NHX基因的耐盐性,从巴西蕉(Musa acuminata L. AAA group)中克隆到一个MaNHXs基因家族的MaNHX5基因,利用生物信息学方法预测了Ma NHX5关键耐盐氨基酸位点和突变前后蛋白质结构的变化,通过定点突变技术将Ma NHX5蛋白的276位丝氨酸(S)成功突变为天冬氨酸(D),利用AXT3盐敏感突变酵母进行功能回补试验。结果表明,将突变后的MaNHX5基因转入AXT3盐敏感突变酵母,200 mmol/L NaCl处理下,突变酵母耐盐性显著提高。由此推测Ma NHX5蛋白的Ser276对香蕉Na+跨液泡膜运输起重要作用。 相似文献
10.
Aspartate kinase (EC 2.7.2.4.) has been purified from 7 day etiolated wheat (Triticum aestivum L. var. Maris Freeman) seedlings and from embryos imbibed for 8 h. The enzyme was 50% inhibited by 0.25 mM lysine. In this study wheat aspartate kinase was not inhibited by threonine alone or cooperatively with lysine; these results contrast with those published previously. In vivo regulation of the synthesis of aspartate-derived amino acids was examined by feeding [14C]acetate and [35S]sulphate to 2–3 day germinating wheat embryos in culture in the presence of exogenous amino acids. Lysine (1 mM) inhibited lysine synthesis by 86%. Threonine (1 mM) inhibited threonine synthesis by 79%. Lysine (1 mM) plus threonine (1 mM) inhibited threonine synthesis by 97%. Methionine synthesis was relatively unaffected by these amino acids, suggesting that there are important regulatory sites other than aspartate kinase and homoserine dehydrogenase. [35S]sulphate incorporation into methionine was inhibited 50% by lysine (2 mM) plus threonine (2 mM) correlating with the reported 50% inhibition of growth by these amino acids in this system. The synergistic inhibition of growth, methionine synthesis and threonine synthesis by lysine plus threonine is discussed in terms of lysine inhibition of aspartate kinase and threonine inhibition of homoserine dehydrogenase.Abbreviations AEC
S-(2-aminoethyl) cysteine 相似文献
11.
Peter M. Spooner Isodore S. Edelman 《Biochimica et Biophysica Acta (BBA)/General Subjects》1976,444(3)
Previous studies indicated that aldosterone enhances active Na+ transport, glycolysis, lactate production and respiration of the toad bladder. Evidence was also presented that the changes in glycolysis and lactate production were secondary to the changes in active Na+ transport. Further analysis of the relationships between metabolism and Na+ transport was undertaken with the aid of two inhibitors of pyruvate metabolism, oxythiamine and phenylpyruvate. These inhibitors prevented the aldosterone-induced increase in oxidation of [6-14C]glucose but had little effect on the increase in lactate production. In contrast, the effect on Na+ transport (i.e., Isc) was completely inhibited by oxythiamine plus phenylpyruvate with glucose as substrate. The effect on Na+ transport, however, was obtained wth the by-pass substrates, oxaloacetate plus ß-hydroxybutyrate, in the presence of these inhibitors. These results implied that steroidal enhancement of lactate production and Na+ transport were independent effects. To evaluate whether an increase in Na+ transport, per se would augment lactate production, the responses were evaluated under conditions of an imposed Na+ gradient (mucosal Na+ = 5 mM; serosal Na+ = 110 mM). Addition of NaCl to the mucosal media evoked the same increase in Isc as the addition of aldosterone; both additions increased Isc more than two-fold. Aldosterone reduced lactate production under these conditions while the re-addition of NaCl had no effect on lactate formation. These results are consistent with an action of aldosterone on pathways involved in oxidative energy metabolism, and suggest that the activation of glycolysis may be a function of the net balance between energy production and utilization. 相似文献
12.
The intracerebro-ventricular administration of human β-Endorphin (β-EP, 0.1–3 μg/rat) or D-alanine2 methionine enkephalinamide (D-ala, 0.3–30 μg/rat) caused a dose dependent reduction in the urine volume. The oliguria was associated with a decrease in the concentration of Na+ and K+ in the urine of rats previously hydrated by oral administration with 25 ml/kg tap water plus 50 ml/kg 0.5% NaCl. On a molar basis, β-EP proved to be about 5–7 times more potent than D-ala. The effects caused by the peptides were antagonized by the simultaneous intraperitoneal administration of 1 mg/kg naloxone. In rats treated chronically with morphine, no cross-tolerance was demonstrated to the antidiuretic effect of β-EP, but clear cross-tolerance was evident to the changes in urine electrolytes induced by β-EP. Results suggest that morphine and the opiate peptides share a similar mechanism of action. 相似文献
13.
Physiological adjustment to salt stress in Jatropha curcas is associated with accumulation of salt ions,transport and selectivity of K+, osmotic adjustment and K+/Na+ homeostasis
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E. N. Silva J. A. G. Silveira C. R. F. Rodrigues R. A. Viégas 《Plant biology (Stuttgart, Germany)》2015,17(5):1023-1029
This study assessed the capacity of Jatropha curcas to physiologically adjust to salinity. Seedlings were exposed to increasing NaCl concentrations (25, 50, 75 and 100 mm ) for 15 days. Treatment without NaCl was adopted as control. Shoot dry weight was strongly reduced by NaCl, reaching values of 35% to 65% with 25 to 100 mm NaCl. The shoot/root ratio was only affected with 100 mm NaCl. Relative water content (RWC) increased only with 100 mm NaCl, while electrolyte leakage (EL) was much enhanced with 50 mm NaCl. The Na+ transport rate to the shoot was more affected with 50 and 100 mm NaCl. In parallel, Cl? transport rate increased with 75 and 100 mm NaCl, while K+ transport rate fell from 50 mm to 100 mm NaCl. In roots, Na+ and Cl? transport rates fell slightly only in 50 mm (to Na+) and 50 and 100 mm (to Cl?) NaCl, while K+ transport rate fell significantly with increasing NaCl. In general, our data demonstrate that J. curcas seedlings present changes in key physiological processes that allow this species to adjust to salinity. These responses are related to accumulation of Na+ and Cl? in leaves and roots, K+/Na+ homeostasis, transport of K+ and selectivity (K–Na) in roots, and accumulation of organic solutes contributing to osmotic adjustment of the species. 相似文献
14.
Active transport of -aminoisobutyric acid (AIB) in Vibrio costicola utilizes a system with affinity for glycine, alanine and, to some extent, methionine. AIB transport was more tolerant of high salt concentrations (3–4 M NaCl) in cells grown in the presence of 1.0 M NaCl than in those grown in the presence of 0.5 M NaCl. The former cells could also maintain much higher ATP contents than the latter in high salt concentrations.Transport kinetic studies performed with bacteria grown in 1.0 M NaCl revealed three effects of the Na+ ion: the first effect is to increase the apparent affinity (K
t) of the transport system for AIB at Na+ concentrations <0.2 M, the second to increase the maximum velocity (V
max) of transport (Na+ concentrations between 0.2 and 1.0 M), and the third to decrease the V
max without affectig K
t (Na+ concentrations >1.0 M). Cells grown in the presence of 0.5 M or 1.0 M NaCl had similar affinity for AIV. Thus, the differences in salt response of transport in these cells do not seem due to differences in AIB binding. Large, transport-inhibitory concentrations of NaCl resulted in efflux of AIB from cells preloaded in 0.5 M or 1.0 M NaCl, with most dramatic efflux occurring from the cells whose AIB transport was more salt-sensitive. Our results suggest that the degree to which high salt concentrations affect the transmembrane electrochemical energy source used for transport and ATP synthesis is an important determinant of salt tolerance.Abbreviations AIB
-aminoisobutyric acid
- pmf
proton motive force 相似文献
15.
Jingyu Sun Junjun Xie Mengliang Niu Zhixiong Liu Molin Fan Zhilong Bie 《Physiologia plantarum》2014,152(4):738-748
Grafting onto salt‐tolerant pumpkin rootstock can increase cucumber salt tolerance. Previous studies have suggested that this can be attributed to pumpkin roots with higher capacity to limit the transport of Na+ to the shoot than cucumber roots. However, the mechanism remains unclear. This study investigated the transport of Na+ in salt‐tolerant pumpkin and salt‐sensitive cucumber plants under high (200 mM) or moderate (90 mM) NaCl stress. Scanning ion‐selective electrode technique showed that pumpkin roots exhibited a higher capacity to extrude Na+, and a correspondingly increased H+ influx under 200 or 90 mM NaCl stress. The 200 mM NaCl induced Na+/H+ exchange in the root was inhibited by amiloride (a Na+/H+ antiporter inhibitor) or vanadate [a plasma membrane (PM) H+‐ATPase inhibitor], indicating that Na+ exclusion in salt stressed pumpkin and cucumber roots was the result of an active Na+/H+ antiporter across the PM, and the Na+/H+ antiporter system in salt stressed pumpkin roots was sufficient to exclude Na+. X‐ray microanalysis showed higher Na+ in the cortex, but lower Na+ in the stele of pumpkin roots than that in cucumber roots under 90 mM NaCl stress, suggesting that the highly vacuolated root cortical cells of pumpkin roots could sequester more Na+, limit the radial transport of Na+ to the stele and thus restrict the transport of Na+ to the shoot. These results provide direct evidence for pumpkin roots with higher capacity to limit the transport of Na+ to the shoot than cucumber roots. 相似文献
16.
D.T. Thwaites D. Markovich H. Murer N.L. Simmons 《The Journal of membrane biology》1996,151(3):215-224
The nature of transepithelial and cellular transport of the dibasic amino acid lysine in human intestinal epithelial Caco-2
cells has been characterized. Intracellular accumulation of lysine across both the apical and basolateral membranes consists
of a Na+-independent, membrane potential-sensitive uptake. Na+-independent lysine uptake at the basolateral membrane exceeds that at the apical membrane. Lysine uptake consists of both
saturable and nonsaturable components. Na+-independent lysine uptake at both membranes is inhibited by lysine, arginine, alanine, histidine, methionine, leucine, cystine,
cysteine and homoserine. In contrast, proline and taurine are without inhibitory effects at both membranes. Fractional Na+-independent lysine efflux from preloaded epithelial layers is greater at the basolateral membrane and shows trans-stimulation
across both epithelial borders by lysine, arginine, alanine, histidine, methionine, and leucine but not proline and taurine.
Na+-independent lysine influx (10 μm) in the presence of 10 mm homoserine shows further concentration dependent inhibition by lysine. Taken together, these data are consistent with lysine
transport being mediated by systems bo,+, y+ and a component of very low affinity (nonsaturable) at both membranes. The relative contribution to lysine uptake at each
membrane surface (at 10 μm lysine), normalized to total apical uptake (100%), is apical bo,+ (47%), y+ (27%) and the nonsaturable component (26%), and basal bo,+ (446%), y+ (276%) and the nonsaturable component (20%). Northern analysis shows hybridization of Caco-2 poly(A)+RNA with a human rBAT cDNA probe.
Received: 3 July 1995/Revised: 6 February 1996 相似文献
17.
A Multisubstrate Kinetic Mechanism of Dopamine Transport in the Nucleus Accumbens and Its Inhibition by Cocaine 总被引:1,自引:1,他引:0
Abstract: Kinetic studies of dopamine transport into suspensions of nucleus accumbens (NAcc) and effects of Na+ and Cl? as cosubstrates were performed using rotating disk electrode voltammetry. To mimic chemical neurotransmission, dopamine was added as a rapid pulse, and transporter-mediated clearance of dopamine was evaluated kinetically. This paradigm was shown to approximate a zero trans entry transport experiment. Dopamine was taken up with apparent Km and Vmax values of 1.3 µM and 375 pmol/s/g wet weight, respectively. Transport exhibited apparent trans acceleration. Substitution of Na+ with choline or Cl? with isethionate reduced dopamine transport with reaction orders of two and unity, respectively, accompanied by reductions in Vmax with no changes in Km. Apparent KNa and KCl values were 70.0 and 92.1 mM, respectively. Dopamine transport in NAcc was found to follow a partially random, sequential mechanism in which dopamine and Na+ bind randomly to the transporter followed by binding of Cl? before transport. Cocaine inhibited dopamine transport and the influences of the other substrates allosterically with an overall Ki of 0.30 µM. Thus, the general kinetic mechanism of the transport of dopamine in the NAcc is identical to that previously reported by this laboratory for dopamine transport in the striatum. However, the dopamine transporter in the NAcc is more tightly regulated by Na+, possesses a higher kinetic turnover rate, is four times more sensitive to cocaine than the striatal transporter, and exhibits cocaine inhibition independent of [substrate]. These findings suggest that cocaine modulates chemical signaling in NAcc differently than in striatum, providing down-regulation of function irrespective of [substrate], thereby enhancing dopaminergic signaling more robustly in the NAcc than in the striatum. 相似文献
18.
Johan A. Hellebust 《Journal of phycology》1978,14(1):79-83
The uptake of glucose and amino acids by the euryhaline diatom Cyclotella cryptica Reimann, Lewin & Guillard does not appear to be related to proton gradients. Instead, the transport systems for these organic solutes show a strong requirement for the presence of NaCl. The relationship between uptake and NaCl concentration is hyperbolic, with optimal uptake rates being approached at 100 mM NaCl. High concentrations of KCl cause strong reductions in uptake rates. The (Na+, K+)-stimulated ATPase inhibitor ouabain has no effect on glucose uptake, whereas the diphenolic glucoside phlorizin and its aglucone phloretin are strongly inhibitory. The proton translocating uncoupler CCCP (carbonylcyanide m-chlorophenyl hydrazone) and the ATPase inhibitor DCCD (dicyclohexylcarbodiimide) both almost completely abolish glucose transport, and low concentrations of the ionophares monensin and valenomycin strongly inhibit glucose uptake by the diatom. The requirement of high external NaCl concentrations for glucose transport, and the inhibitory effect an transport of the Na+-specific ionophore monensin are consistent with a coupling of Na+ and organic substrate transport, but could also be explained by a Na+ requirement for glucose binding to a transport carrier, and/or a possible interference with energy producing reactions associated with a monensin-induced collapse of the normal Na+ gradient. 相似文献
19.
A study was performed to correlate regional morphology and amiloride inhibitable Na+‐transport in the coprodeal epithelium in hens, Gallus domesticus, on low‐NaCl diet and in controls. Proximal (close to colon), mid and distal (close to urodeum) regions were examined using light microscopy, transmission‐ and scanning electron microscopy. Na+‐transport was measured electrophysiologically in Ussing‐chambers in the proximal and distal regions. The epithelium, simple and columnar, is composed of absorptive intestinal epithelial cells, goblet cells, brush cells, migrating lymphoid cells, and entero‐endocrine cells. Brush cells, identified in avians for the first time, occur in highest number in the proximal part of the coprodeum in low‐NaCl hens. Na+‐transport is high in the low‐NaCl hens, ranging from 347μA/cm2 (proximal) to 187μA/cm2 (distal). In control hens, which correspond to hens on high‐NaCl diet, it is low in all regions (0–4 μA/cm2). Absorptive intestinal epithelial cells as well as brush cells adapt to variations in transepithelial Na+‐transport by regulating height and packing density of their microvilli, number, size, and localization of apical vesicles, and the width of the intercellular space. Regional differences in the epithelial cell composition and ultrastructure are closely correlated to transepithelial Na+‐transport but only in low‐NaCl hens, as controls do not show these variations. J. Morphol. 239:75–86, 1999. © 1999 Wiley‐Liss, Inc. 相似文献
20.
The moderate halophile Vibrio costicola, growing on a chemically-defined medium, transformed choline into glycine betaine (betaine) by the membrane-bound enzyme choline dehydrogenase and the cytoplasmic enzyme betainal (betaine aldehyde) dehydrogenase. Choline dehydrogenase was strongly induced and betainal dehydrogenase less strongly induced by choline. The formation of these enzymes was also regulated by the NaCl concentration of the growth medium, increasing with increasing NaCl concentrations. Intracellular betaine concentrations also increased with increasing choline and NaCl concentrations in the medium. This increase was almost completely blocked by chloramphenicol, which does not block the increase in salt-tolerant active transport on transfer from a low to a high salt concentration.Choline dehydrogenase was inhibited by chloride salts of Na+, K+, and NH
inf4
su+
, the inhibition being due to the Cl- ions. Betainal dehydrogenase was stimulated by 0.5 M salts and could function in up to 2.0 M salts.Cells grew as well in the presence as in the absence of choline in 0.5 M and 1.0 M NaCl, but formed no intracellular betaine. Choline stimulated growth in 2.0 M NaCl and was essential for growth in 3.0 M NaCl. Thus, while betaine is important for some of the adaptations to high salt concentration by V. costicola, it by no means accounts for all of them.Abbreviations CDMM
chemically-defined minimal medium
- PPT
proteose-peptone tryptone medium
- SDS
sodium dodecyl sulfate
Deceased, 1987 相似文献