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1.
A highly enriched denitrifying mixed culture transformedo-xylene cometabolically along with toluene by methyl group oxidation.o-Methyl benzaldehyde ando-methyl benzoic acid accumulated transiently as metabolic products ofo-xylene transformation. Transformation ofo-methyl benzyl alcohol ando-methyl benzaldehyde occurred independently of toluene degradation and resulted in the formation of a compound coeluting witho-methyl benzoic acid on a gas chromatograph. The cometabolic relationship between toluene ando-xylene could be attributed to a mechanism linked to the initial oxidation of the methyl group. 相似文献
2.
Anaerobic degradation of ethylbenzene and other aromatic hydrocarbons by new denitrifying bacteria 总被引:29,自引:0,他引:29
Anaerobic degradation of alkylbenzenes with side chains longer than that of toluene was studied in freshwater mud samples in the presence of nitrate. Two new denitrifying strains, EbN1 and PbN1, were isolated on ethylbenzene and n-propylbenzene, respectively. For comparison, two further denitrifying strains, ToN1 and mXyN1, were isolated from the same mud with toluene and m-xylene, respectively. Sequencing of 16SrDNA revealed a close relationship of the new isolates to Thauera selenatis. The strains exhibited different specific capacities for degradation of alkylbenzenes. In addition to ethylbenzene, strain EbN1 utilized toluence, but not propylbenzene. In contrast, propylbenzene-degrading strain PbN1 did not grow on toluene, but was able to utilize ethylbenzene. Strain ToN1 used toluene as the only hydrocarbon substrate, whereas strain mXyN1 utilized both toluene and m-xylene. Measurement of the degradation balance demonstrated complete oxidation of ethylbenzene to CO2 by strain EbN1. Further characteristic substrates of strains EbN1 and PbN1 were 1-phenylethanol and acetophenone. In contrast to the other isolates, strain mXyN1 did not grow on benzyl alcohol. Benzyl alcohol (also m-methylbenzyl alcohol) was even a specific inhibitor of toluene and m-xylene utilization by strain mXyN1. None of the strains was able to grow on any of the alkylbenzenes with oxygen as electron acceptor. However, polar aromatic compounds such as benzoate were utilized under both oxic and anoxic conditions. All four isolates grew anaerobically on crude oil. Gas chromatographic analysis of crude oil after growth of strain ToN1 revealed specific depletion of toluene. 相似文献
3.
Pseudomonas stutzeri OX1 is able to grow ono-xylene but is unable to grow onm-xylene andp-xylene, which are partially metabolized through theo-xylene degradative pathway leading to the formation of dimethylphenols toxic to OX1.P. stutzeri spontaneous mutants able to grow onm-xylene andp-xylene have been isolated. These mutants soon lose the ability to grow ono-xylene. Data from HPLC analyses and from induction studies suggest that in these mutantsm-xylene andp-xylene could be metabolized through the oxidation of a methyl substituent.P. stutzeri chromosomal DNA is shown to share homology with pWW0 catabolic genes. In the mutant strains the region homologous to pWW0 upper pathway genes has undergone a genomic rearrangement.Abbreviations BADH
benzylalcohol dehydrogenase
- cat
catechol
- C23O
catechol 2,3-dioxygenase
- 2,3-,3,4-,2,4-,2,6-,3,5-2,5-DMP
2,3-,3,4-,2,4-,2,6-,3,5-,2,5-dimethylphenol
- 2-MBOH
2-methylbenzyl alcohol
- 3-MBOH
3-methylbenzyl alcohol
- 4-MBOH
4-methylbenzyl alcohol
-
m-,p-tol
m-,p-toluate
-
o-,m-,p-xyl
o-,m-,p-xylene 相似文献
4.
Anaerobic degradation of p-xylene in sediment-free sulfate-reducing enrichment culture 总被引:1,自引:1,他引:0
Anaerobic degradation of p-xylene was studied with sulfate-reducing enrichment culture. The enrichment culture was established with sediment-free sulfate-reducing
consortium on crude oil. The crude oil-degrading consortium prepared with marine sediment revealed that toluene, and xylenes
among the fraction of alkylbenzene in the crude oil were consumed during the incubation. The PCR-denaturing gradient gel electrophoresis
(DGGE) analysis of 16S rRNA gene for the p-xylene degrading sulfate-reducing enrichment culture showed the presence of the single dominant DGGE band pXy-K-13 coupled
with p-xylene consumption and sulfide production. Sequence analysis of the DGGE band revealed a close relationship between DGGE
band pXy-K-13 and the previously described marine sulfate-reducing strain oXyS1 (similarity value, 99%), which grow anaerobically
with o-xylene. These results suggest that microorganism corresponding to pXy-K-13 is an important sulfate-reducing bacterium to
degrade p-xylene in the enrichment culture. 相似文献
5.
A two-phase aqueous/organic partitioning bioreactor scheme was used to degrade mixtures of toluene and benzene, and toluene
and p-xylene, using simultaneous and sequential feeding strategies. The aqueous phase of the partitioning bioreactor contained
Pseudomonas sp. ATCC 55595, an organism able to degrade benzene, toluene and p-xylene simultaneously. An industrial grade of oleyl alcohol served as the organic phase. In each experiment, the organic
phase of the bioreactor was loaded with 10.15 g toluene, and either 2.0 g benzene or 2.1 g p-xylene. The resulting aqueous phase concentrations were 50 mg/l, 25 mg/l and 8 mg/l toluene, benzene and p-xylene respectively. The simultaneous fermentation of benzene and toluene consumed these compounds at volumetric rates of
0.024 g l−1 h−1 and 0.067 g l−1 h−1, respectively. The simultaneous fermentation of toluene and p-xylene consumed these xenobiotics at volumetric rates of 0.066 g l−1 h−1 and 0.018 g l−1 h−1, respectively. A sequential feeding strategy was employed in which toluene was added initially, but the benzene or p-xylene aliquot was added only after the cells had consumed half of the initial toluene concentration. This strategy was shown
to improve overall degradation rates, and to reduce the stress on the microorganisms. In the sequential fermentation of benzene
and toluene, the volumetric degradation rates were 0.056 g l−1 h−1 and 0.079 g l−1 h−1, respectively. In the toluene/p-xylene sequential fermentation, the initial toluene load was consumed before the p-xylene aliquot was consumed. After 12 h in which no p-xylene degradation was observed, a 4.0-g toluene aliquot was added, and p-xylene degradation resumed. Excluding that 12-h period, the microbes consumed toluene and p-xylene at volumetric rates of 0.074 g l−1 h−1 and 0.025 g l−1 h−1, respectively. Oxygen limitation occurred in all fermentations during the rapid growth phase.
Received: 16 November 1998 / Received revision: 29 March 1999 / Accepted: 9 April 1999 相似文献
6.
Anaerobic toluene oxidation by the sulfate-reducing bacterium, strain Tol2 (proposed nameDesulfobacula toluolica) was specifically inhibited by benzyl alcohol when added at concentrations around 500 μM. Benzyl alcohol added at lower,
non-inhibitory concentrations (around 5 μM) was not oxidized by active cells pregrown on toluene, indicating that the alcohol
is not a free intermediate of toluene metabolism in the sulfate reducer. Conversion ofp-xylene in toluene-metabolizing cells top-methylbenzoate as dead-end product suggests that the sulfate reducer, like denitrifiers, initiates toluene oxidation at the
methyl group. 相似文献
7.
In the course of study on the utilization of methyl-substituents of mono-cyclic aromatic hydrocarbons by Pseudomonas aeruginosa S668B2, some organic acids and phenolic compounds were found to be produced in culture broth.Strain S668B2 was capable of producing ultraviolet absorbing and fluorescent substances from m-xylene. These substances were isolated in the form of crystal and identified as 3-methyl salicylic acid and m-toluic acid.Strain S668B2 also produced ultraviolet absorbing and fluorescent substances from pseudocumene (1,2,4-trimethyl benzene). These substances were isolated in the crystalline form and identified as 3,4-dimethyl benzoic acid and 3,4-dimethyl phenol.Strain S668B″ did not attack o-xylene. Under the similar conditions Pseudomonas desmolytica S449B3, which produced a large amount of cumic acid from p-cymene, did not oxidize o-xylene, but grew on p-xylene, m-xylene and 1,2,4-trimethyl benzene.None out of 364 soil samples gave microorganisms which utilize o-xylene as a sole carbon source. 相似文献
8.
Benzene, toluene, ethylbenzene and xylene (BTEX) substrate interactions for a mesophilic (25°C) and thermophilic (50°C) toluene-acclimatized composted pine bark biofilter were investigated. Toluene, benzene, ethylbenzene, o-xylene, m-xylene and p-xylene removal efficiencies, both individually and in paired mixtures with toluene (1:1 ratio), were determined at a total loading rate of 18.1 g m–3 h–1 and retention time ranges of 0.5–3.0 min and 0.6–3.8 min for mesophilic and thermophilic biofilters, respectively. Overall, toluene degradation rates under mesophilic conditions were superior to degradation rates of individual BEX compounds. With the exception of p-xylene, higher removal efficiencies were achieved for individual BEX compounds compared to toluene under thermophilic conditions. Overall BEX compound degradation under mesophilic conditions was ranked as ethylbenzene >benzene >o-xylene >m-xylene >p-xylene. Under thermophilic conditions overall BEX compound degradation was ranked as benzene >o-xylene >ethylbenzene >m-xylene >p-xylene. With the exception of o-xylene, the presence of toluene in paired mixtures with BEX compounds resulted in enhanced removal efficiencies of BEX compounds, under both mesophilic and thermophilic conditions. A substrate interaction index was calculated to compare removal efficiencies at a retention time of 0.8 min (50 s). A reduction in toluene removal efficiencies (negative interaction) in the presence of individual BEX compounds was observed under mesophilic conditions, while enhanced toluene removal efficiency was achieved in the presence of other BEX compounds, with the exception of p-xylene under thermophilic conditions. 相似文献
9.
Purification and characterisation of TOL plasmid-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase of Pseudomonas putida 总被引:3,自引:0,他引:3
Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase, two enzymes of the xylene degradative pathway encoded by the plasmid TOL of a Gram-negative bacterium Pseudomonas putida, were purified and characterized. Benzyl alcohol dehydrogenase catalyses the oxidation of benzyl alcohol to benzaldehyde with the concomitant reduction of NAD+; the reaction is reversible. Benzaldehyde dehydrogenase catalyses the oxidation of benzaldehyde to benzoic acid with the concomitant reduction of NAD+; the reaction is irreversible. Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase also catalyse the oxidation of many substituted benzyl alcohols and benzaldehydes, respectively, though they were not capable of oxidizing aliphatic alcohols and aldehydes. The apparent Km value of benzyl alcohol dehydrogenase for benzyl alcohol was 220 microM, while that of benzaldehyde dehydrogenase for benzaldehyde was 460 microM. Neither enzyme contained a prosthetic group such as FAD or FMN, and both enzymes were inactivated by SH-blocking agents such as N-ethylmaleimide. Both enzymes were dimers of identical subunits; the monomer of benzyl alcohol dehydrogenase has a mass of 42 kDa whereas that of the monomer of benzaldehyde dehydrogenase was 57 kDa. Both enzymes transfer hydride to the pro-R side of the prochiral C4 of the pyridine ring of NAD+. 相似文献
10.
We have examined aspects of the second catalytic activity of alcohol dehydrogenase from horse liver (LADH), which involves an apparent dismutation of an aldehyde substrate into alcohol and acid in the presence of LADH and NAD. Using the substrate p-trifluoromethylbenzaldehyde, we have observed various bound complexes by 19F NMR in an effort to further characterize the mechanism of the reaction. The mechanism appears to involve the catalytic activity of LADH · NAD · aldehyde complex which reacts to form an enzyme · NADH · acid complex. The affinity of the acid product for LADH · NADH is weak and the acid product readily desorbs from the ternary complex. The resulting LADH · NADH can then react with a second molecule of aldehyde to form NAD and the corresponding alcohol. The result is the conversion of two molecules of aldehyde to one each of acid and alcohol, with LADH and NAD acting catalytically. This sequence of reactions can also explain the slow formation of acid product observed when alcohol and NAD are incubated with the enzyme. 相似文献
11.
Microbial Hydrocarbon Co-oxidation. III. Isolation and Characterization of an alpha, alpha'-Dimethyl-cis, cis-Muconic Acid-producing Strain of Nocardia corallina
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A soil isolate identified as a strain of Nocardia corallina accumulated α, α′-dimethyl-cis, cis-muconic acid under co-oxidation conditions employing n-hexadecane for growth and p-xylene as the co-oxidizable substrate. N. corallina V-49 was postulated to have two pathways for the oxidation of p-xylene. One pathway proceeds throughp-benzyl alcohol, p-tolualdehyde, and p-toluic acid to 2, 3-dihydroxy-p-toluic acid, and the other pathway results in ortho ring cleavage of 3, 6-dimethylpyrocatechol and hence accumulation of α,α′-dimethyl-cis, cis-muconic acid. 相似文献
12.
A soil isolate identified as a strain of Nocardia corallina accumulated α, α′-dimethyl-cis, cis-muconic acid under co-oxidation conditions employing n-hexadecane for growth and p-xylene as the co-oxidizable substrate. N. corallina V-49 was postulated to have two pathways for the oxidation of p-xylene. One pathway proceeds throughp-benzyl alcohol, p-tolualdehyde, and p-toluic acid to 2, 3-dihydroxy-p-toluic acid, and the other pathway results in ortho ring cleavage of 3, 6-dimethylpyrocatechol and hence accumulation of α,α′-dimethyl-cis, cis-muconic acid. 相似文献
13.
BTEX catabolism interactions in a toluene-acclimatized biofilter 总被引:1,自引:0,他引:1
BTEX substrate interactions for a toluene-acclimatized biofilter consortium were investigated. Benzene, ethylbenzene, o-xylene, m-xylene and p-xylene removal efficiencies were determined at a loading rate of 18.07 g m−3 h−1 and retention times of 0.5–3.0 min. This was also repeated for toluene in a 1:1 (m/m) ratio mixture (toluene: benzene, ethylbenzene,
or xylene ) with each of the other compounds individually to obtain a final total loading of 18.07 g m−3 h−1. The results obtained were modelled using Michaelis–Menten kinetics and an explicit finite difference scheme to generate
v
max and K
m parameters. The v
max/K
m ratio (a measure of the catalytic efficiency, or biodegradation capacity, of the reactor) was used to quantify substrate
interactions occurring within the biofilter reactor without the need for free-cell suspended and monoculture experimentation.
Toluene was found to enhance the catalytic efficiency of the reactor for p-xylene, while catabolism of all the other compounds was inhibited competitively by the presence of toluene. The toluene-acclimatized
biofilter was also able to degrade all of the other BTEX compounds, even in the absence of toluene. The catalytic efficiency
of the reactor for compounds other than toluene was in the order: ethylbenzene>benzene>o-xylene>m-xylene>p-xylene. The catalytic efficiency for toluene was reduced by the presence of all other tested BTEX compounds, with the greatest
inhibitory effect being caused by the presence of benzene, while o-xylene and p-xylene caused the least inhibitory effect. This work illustrated that substrate interactions can be determined directly from
biofilter reactor results without the need for free-cell and monoculture experimentation.
Received: 13 April 2000 / Received revision: 20 July 2000 / Accepted: 27 July 2000 相似文献
14.
Summary A series of investigations were conducted with the objective of elucidating natural pathways of electron transport from respiratory
processes to the site of N2 fixation in nodule bacteroids. A survey of dehydrogenase activities in a crude extract of soybean nodule bacteroids revealed
relatively high activities of NAD-specific β-hydroxybutyrate and glyceraldehyde-3-phosphate dehydrogenases. Moderate activities
of NADP-specific isocitrate and glucose-6-phosphate dehydrogenases were observed. By use of the ATP-dependent acetylene reduction
reaction catalyzed by soybean bacteroid nitrogenase, and enzymes and cofactors from bacteroids and other sources, the following
sequences of electron transport to bacteroid nitrogenase were demonstrated: (1) H2 to bacteroid nitrogenase in presence of a nitrogenase-free extract ofC. pasteurianum; (2) β-hydroxybutyrate to bacteroid nitrogenase in a reaction containing β-hydroxybutyrate dehydrogenase, NADH dehydrogenase,
NAD and benzyl viologen; (3) β-hydroxybutyrate dehydrogenase, to nitrogenase in reaction containing NADH dehydrogenase, NAD
and either FMN or FAD; (4) light-dependent transfer of electrons from ascorbate to bacteroid nitrogenase in a reaction containing
photosystem I from spinach chloroplasts, 2,6-dichlorophenolindophenol, and either azotoflavin from Azotobacter or non-heme
iron protein from bacteroids; (5) glucose-6-phosphate to bacteroid nitrogenase in a system that included glucose-6-phosphate
dehydrogenase, NADP, NADP-ferredoxin reductase from spinach, azotoflavin from Azotobacter and bacteroid non-heme iron protein.
The electron transport factors, azotoflavin and bacteroid non-heme iron protein, failed to function in the transfer of electrons
from an NADH-generating system to bacteroid nitrogenase. When FMN or FAD were added to systems containing azotoflavin and
bacteroid non-heme iron protein, electrons apparently were transferred to the flavin-nucleotides and then nitrogenase without
involvement of azotoflavin and bacteroid non-heme iron protein.
Evidence is available indicating that nodule bacteroids contain flavoproteins analogous to Azotobacter, azotoflavin, and spinach
ferredoxin-NADP reductase. It is concluded that physiologically important systems involved in transport of electrons from
dehydrogenases to nitrogenase in bacteroids very likely will include relatively specific electron transport proteins such
as bacteroid non-heme iron protein and a flavoprotein from bacteroids that is analogous to azotoflavin. 相似文献
15.
A two-phase organic/aqueous reactor configuration was developed for use in the biodegradation of benzene, toluene and p-xylene, and tested with toluene. An immiscible organic phase was systematically selected on the basis of predicted and experimentally
determined properties, such as high boiling points, low solubilities in the aqueous phase, good phase stability, biocompatibility,
and good predicted partition coefficients for benzene, toluene and p-xylene. An industrial grade of oleyl alcohol was ultimately selected for use in the two-phase partitioning bioreactor. In
order to examine the behavior of the system, a single-component fermentation of toluene was conducted with Pseudomonas sp. ATCC 55595. A 0.5-l sample of Adol 85 NF was loaded with 10.4 g toluene, which partitioned into the cell containing 1 l
aqueous medium at a concentration of approximately 50 mg/l. In consuming the toluene to completion, the organisms were able
to achieve a volumetric degradation rate of 0.115 g l−1 h−1. This system is self-regulating with respect to toluene delivery to the aqueous phase, and requires only feedback control
of temperature and pH.
Received: 16 November 1998 / Received revision: 28 March 1999 / Accepted: 9 April 1999 相似文献
16.
Hernando Pactao Bacosa Koichi Suto Chihiro Inoue 《World journal of microbiology & biotechnology》2011,27(5):1109-1117
In this study, the abilities of two microbial consortia (Y and F) to degrade aliphatic–aromatic hydrocarbon mixtures were
investigated. Y consortium preferentially degraded the aromatic hydrocarbon fractions in kerosene, while F consortium preferentially
degraded the aliphatic hydrocarbon fractions. Degradation experiments were performed under aerobic conditions in sealed bottles
containing liquid medium and n-octane or n-decane as representative aliphatic hydrocarbons or toluene, ethylbenzene or p-xylene as representative aromatic hydrocarbons (all at 100 mg/l). Results demonstrated that the Y consortium degraded p-xylene more rapidly than n-octane. It degraded toluene, ethylbenzene and p-xylene more rapidly than decane. In comparison, the F consortium degraded n-octane more rapidly than toluene, ethylbenzene or p-xylene, and n-decane more rapidly than toluene, ethylbenzene or p-xylene. 16S rRNA gene sequencing revealed that the Y consortium was dominated by Betaproteobacteria and the F consortium by Gammaproteobacteria, and in particular Pseudomonas. This could account for their metabolic differences. The substrate preferences of the two consortia showed that the aliphatic–aromatic
hydrocarbon binary mixtures, especially the n-decane–toluene/ethylbenzene/p-xylene pairs, reflected their degradation ability of complex hydrocarbon compounds such as kerosene. This suggests that aliphatic–aromatic
binary systems could be used as a tool to rapidly determine the degradation preferences of a microbial consortium. 相似文献
17.
Gas-liquid chromatographic study revealed that organophosphorus fungicide Inezin® (S-benzyl O-ethyl phenylphosphonothioate) was metabolized to O-ethyl hydrogen phenylphosphonothioate or ethyl hydrogen phenylphosphonate by mycelial cells of Pyricularia oryzae. Metabolic fate of the removed benzyl or benzylthio group was further studied by labeling benzene ring of benzyl radical of the fungicide with 14C, and dibenzyl disulfide, benzyl alcohol, toluene-α-sulfonic acid and benzoic acid were found as metabolites by ion exchange chromatography and thin-layer chromatography. Gas-liquid chromatographic study also ascertained that a part of Inezin was hydroxylated at m-position of the benzene ring of benzyl radical, but o- or p-hydroxylation of benzyl radical was not seemed to occur.No significant difference was found in metabolism of the fungicide between susceptible and resistant clones of the fungi. 相似文献
18.
Thomas Biegert Uwe Altenschmidt Christoph Eckerskorn Georg Fuchs 《Archives of microbiology》1995,163(6):418-423
Toluene and related aromatic compounds are anaerobically degraded by the denitrifying bacterium Thauera sp. strain K172 via oxidation to benzoyl-CoA. The postulated initial step is methylhydroxylation of toluene to benzyl alcohol, which is either a free or enzyme-bound intermediate. Cells grown with toluene or benzyl alcohol contained benzyl alcohol dehydrogenase, which is possibly the second enzyme in the proposed pathway. The enzyme was purified from benzyl-alcohol-grown cells and characterized. It has many properties in common with benzyl alcohol dehydrogenase from Acinetobacter and Pseudomonas species. The enzyme was active as a homotetramer of 160kDa, with subunits of 40kDa. It was NAD+-specific, had an alkaline pH optimum, and was inhibited by thiol-blocking agents. No evidence for a bound cofactor was obtained. Various benzyl alcohol analogues served as substrates, whereas non-aromatic alcohols were not oxidized. The N-terminal amino acid sequence indicates that the enzyme belongs to the class of long-chain Zn2+-dependent alcohol dehydrogenases, although it appears not to contain a metal ion that can be removed by complexing agents.Dedicated to Prof. Achim Trebst 相似文献
19.
Vanderberg LA Krieger-Grumbine R Taylor MN 《Applied microbiology and biotechnology》2000,53(4):447-452
Rhodococcus rhodochrous strain OFS grew on toluene as a sole source of carbon and energy with a maximum growth rate of 0.011 h−1. Initial reaction products were extracted, derivatized and identified by GC-MS. Oxygen consumption studies indicated that
OFS grown on an aliphatic substrate required an induction period before oxidizing toluene. OFS grown on toluene transformed
an array of aromatic ground water pollutants including styrene, ethylbenzene and chlorobenzene. Products of these transformations
were identified. The sole product of chlorobenzene biotransformation was 4-chlorophenol. Products from toluene oxidation included
3- and 4-methylcatechol as well as benzyl alcohol, p-cresol and cis-toluene dihydrodiol. The identification of these and the products of other aromatic substrate conversions affirm that oxidation
occurred on the functional group as well as directly on the aromatic nucleus.
Received: 23 July 1999 / Received revision: 4 October 1999 / Accepted: 16 October 1999 相似文献
20.
Toluene and related aromatic compounds can be mineralized to CO2 under anoxic conditions. Oxidation requires new dehydrogenase-type enzymes and water as oxygen source, as opposed to the aerobic enzymatic attack by oxygenases, which depends on molecular oxygen. We studied the anaerobic process in the denitrifying bacterium Thauera sp. strain K172. Toluene and a number of its fluoro-, chloro- and methyl-analogues were transformed to benzoate and the respective analogues by whole cells and by cell extracts. The transformation of xylene isomers to methylbenzoate isomers suggests that xylene degradation is similarly initiated by oxidation of one of the methyl groups. Toluene oxidation was strongly, but reversibly inhibited by benzyl alcohol. The in vitro oxidation of the methyl group was coupled to the reduction of nitrate, required glycerol for activity, and was inhibited by oxygen. Cells also contained benzyl alcohol dehydrogenase (NAD+), benzaldehyde dehydrogenase (NADP+), benzoate-CoA ligase (AMP-forming), and benzoyl-CoA reductase (dearomatizing). The toluene-oxidizing activity was induced when cells were grown anaerobically with toluene and also with benzyl alcohol or benzaldehyde, suggesting that benzyl alcohol or benzaldehyde acts as inducer. The other enzymes were similarly active in cells grown with toluene, benzyl alcohol, benzaldehyde, or benzoate. This is the first in vitro study of anaerobic oxidation of an aromatic hydrocarbon and of the whole-cell regulation of the toluene-oxidizing enzyme.Dedicated to Prof. Achim Trebst 相似文献