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1.
The coarse of growth and cell division in synchronized cultures of Chlorella pyrenoidosa was studied after the addition of metabolic inhibitors at differing times during the cell cycle (14 h light - 10 h darkness with nitrate as nitrogen source. 12 h light: 12 h darkness with urea as nitrogen source). Dinitrophenol (DNP) added to a final concentration of 0.3 mM at any time in the synchronization cycle, the compound remaining in the suspension from the time of addition to the end of the dark period, inhibited spore formation completely. Growth measured as increase in cell volume was less sensitive to the action of the inhibitor. Chloramphenicol (CAP) added dining the 0–5 h interval to a final concentration of 0.1 mM resulted in 80 per cent inhibition of cell division. Similar treatment started at successive times thereafter resulted in a gradual decrease of the inhibition. Treatment at the 14th hour and during the dark period did not affect the sporulation. Similar experiments with 0.9 mM puromycin added at various times during the illumination period gave almost complete inhibition of cell division, while the growth was reduced by only 25 per cent. para-Fluorophenylalanine (p-FPhe) at 3.3 × 10?2 mM stopped cell division nearly completely irrespective of addition time in the light period. Addition during the dark period also prevented an increase in the number of tree cells. In this case about half of the cells produced spores which were not released. It is concluded that DNP inhibits all stages of preparation for cell division, as well as the division process itself. With CAP a genuine transition point of preparation for cell division was observed, although its interpretation as related to protein synthesis is somewhat uncertain. With puromycin and p-FPhe no transitions were observed. 相似文献
2.
Cell division of the Escherichia coli lon- mutant 总被引:14,自引:0,他引:14
Summary
Escherichia coli lon
-cells were subjected to treatments which produced a decrease in the DNA/mass ratio of the cell. Thymine starvation, a shift-up from minimal medium to rich medium, and exposure to BUdR each caused greater inhibition of cell division in lon
-cells than in lon
+cells. DNA metabolism was found to be the same in both lon
+and lon
-cells during these treatments. The results are consistent with the hypothesis that the lon
-defect leads to inhibition of cell division under conditions which produce a decreased DNA/mass ratio. 相似文献
3.
Leaf growth consists of two basic processes, cell division and cell enlargement. DNA synthesis is an integral part of cell division and can be studied with autoradiographic techniques and incorporation of some labeled precursor. Studies were made on the synthesis of nuclear DNA through incorporation of 3H-thymidine in various parts of the lamina during the entire course of leaf development of Xanthium pennsylvanicum. The time course analysis of DNA synthesis was correlated with cell division and rates of cell enlargement. Significant differences in 3H-thymidine incorporation were found in various parts of the lamina. Cell division and DNA synthesis were highest in the early stages of development. Since no 3H-thymidine was incorporated after cessation of cell division (LPI 2.8) in the leaf lamina, it appears that DNA synthesis is not needed for enlargement and differentiation of Xanthium cells. Rates of cell enlargement were negligible in the early development and reached their maximum after cessation of mitoses, between plastochron ages (LPI) 3 and 4. Cells matured between LPI's 5 and 6. Enzymatic activity was correlated with cell division and cell differentiation at various stages of leaf development. 相似文献
4.
Yoshihiro Yoshiyama Hitoshi Shimoii Gakuzo Tamura 《Bioscience, biotechnology, and biochemistry》2013,77(8):1743-1751
Substances which restore impaired cell division in UV-irradiated E. coli B were surveyed among various bacteria. The active substance was found only in several genera of Gram-negative bacteria, i.e., Escherichia, Enterobacter, Salmonella and some species of Pseudomonas. The activity in the dialyzed cell extract of E. coli B/r was observed in the presence of β-NAD and was enhanced by Mg2 + and Mn2+. The active substance was very labile, but the activity was protected by 1 mM dithiothreitol in the process of purification. The activity of a fraction recovered through DEAE-cellulose column chromatography was stimulated by the presence of membrane fraction. Upon treatment with lipid-degrading enzymes and proteases, the division-stimulating activity was lost or reduced. It appears that the inactivation by lipase and phospholipase A2 was due to the formation of lysophospholipids and that a proteinous substance participated in the recovery of impaired cell division of UV-irradiated E. coli B. 相似文献
5.
SYNOPSIS. Action of mercaptoethanol (ME) on cell division and macromolecular synthesis was examined in Tetrahymena synchronized for division. Cells continuously exposed to increasingly higher concentrations of ME divided with progressively longer division delays showing a dosage-dependent response to the agent. Division was blocked in 2 × 10?2 M ME. Many cells cytolyzed in high concentrations of ME (4 × 10?2 M); others became spherical and motility decreased. Non-delaying concentrations of ME (2 × 10?2 M) had little or no effect on protein synthesis but decreased DNA and RNA synthesis 10 and 35%, respectively. Blocking concentrations inhibited incorporation of phenylalanine, thymidine and uridine 35, 60, and 85%, respectively. It is suggested that the mode of action of ME is mediated thru inhibition of macromolecular synthesis essential for cell division and thru inhibition of formation of disulfide bridges between protein subunits. 相似文献
6.
Cation Metabolism in Relation to Cell Size in Synchronously Grown Tissue Culture Cell 总被引:3,自引:1,他引:2
In randomly grown tissue culture cells (mouse leukemic lymphoblast, L5178Y) the number, volume, and Na+ and K+ content increase as an exponential function with a doubling time of 11.3 hr. In synchronously grown cells the volume increase of the population and of single cells follows the same exponential function as in randomly grown cells. In contrast, the cation content fluctuates during a single cell cycle. About 1½ hr after the cell division burst (at the beginning of the S period), a net loss of K+ occurs for a period of about 1 hr amounting to about 20% of the total K. Over the next 5 to 6 hr, the deficit in K+ is eliminated. The Na+ content shows a double fluctuation. It falls during the cell division burst, rises when the K+ content decreases, falls again when K+ content rises, and then increases again before the next cell division burst. The net fluxes of both Na+ and K+ are very small compared to the unidirectional fluxes (less than 5%), thus small changes in the balance of influx and efflux account for the changes in cation content during the growth cycle. Both unidirectional fluxes increase dramatically (by a factor of two) about 2 hr after the cell division burst, and then remain constant until after the next cell division. The pattern of electrolyte regulation during cell division does not follow a simple function such as cell number, cell surface, or cell volume, but must be related to specific internal events in the cell. 相似文献
7.
Kenshi Suzuki 《Molecular & general genetics : MGG》1974,129(3):249-258
Summary Cell division and incorporation of 3H-thymidine into acid-insoluble fraction were investigated for three uvrA recA double mutants of E. coli K12 irradiated with UV at 1.5 ergs/mm2, producing about ten pyrimidine dimers per genome (about 0.01% survival). Cell division was measured both in M9 medium and in the same medium which was made very viscous by the addition of Metlose (the same product as Methocel used by Lin et al., 1971). It was found that a major fraction of irradiated bacteria continues to divide once or twice and stops thereafter. Incorporation of 3H-thymidine proceeded at a considerable rate for a short period following irradiation and then stopped. During subsequent incubation, the incorporation gradually decreased and after 4 h incubation most of the early incorporated radioactivity disappeared from the acid-insoluble fraction. These results indicate that cell division occurs after irradiation without parallel DNA synthesis as in a recA thy mutant of E. coli K12 deprived of thymine (Inouye, 1971). These results suggest that UV irradiation increases lethal sectoring due to the reckless cell division without parallel DNA synthesis. Since DNA synthesis took place only for a short period after irradiation, it may be assumed that the recA gene normally has at least a dual function; 1. elimination of damage induced by UV to support elongation or initiation of DNA, and 2. maintenance of coordination between DNA synthesis and cell division. 相似文献
8.
The effect of different concentrations of cadmium on the viability, cell division, and the total increase in the biomass of
the VBI-O cell strain of tobacco (Nicotiana tabacumL., Virginia Bright Italia) was followed.
The concentration of 10-6 mol 1-1 Cd2+ was fully tolerated by this strain, a nearly total inhibition of cell division and high cell mortality rate ocsicurred at
the concentration of 10-4 mol1{si-1} Cd2+.
Following a long-term exposure of the culture to gradually increasing cadmium concentrations, seven cell lines able to grow
on media containing 10-4 mol 1-1 Cd2+ were derived. Phenotype diversity of the isolated cell lines likely causes of the disappearance of the resistance character
are discussed. 相似文献
9.
SHUZO KUDO SEIJI NAGAO REIKO KASAI YOSHINORI NOZAWA 《The Journal of eukaryotic microbiology》1981,28(2):165-167
Guanylate cyclase activity decreased during the division phase of heat-shock synchronized Tetrahymena pyriformis, strain GL. However, when Ca2+ was removed by EGTA to negate the effects of the Ca2+-binding protein (calmodulin), which is required for the full activity of guanylate cyclase in this organism, no significant change in the enzymatic activity was observed throughout the cell cycle. On the other hand, the reduced guanylate cyclase activity at division phase was associated with a decreased level of calmodulin content. These results suggest that fluctuations in guanylate cyclase activity during the cell cycle would be dependent on the concentration of calmodulin. 相似文献
10.
Radioisotopic Method for Measuring Cell Division Rates of Individual Species of Diatoms from Natural Populations 总被引:1,自引:0,他引:1
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Richard B. Rivkin 《Applied microbiology》1986,51(4):769-775
Silicon is an essential element for diatom frustule synthesis and is usually taken up only by dividing cells. With 68Ge, a radioactive analog of Si, the cell cycle marker event of frustule formation was identified for individual species of diatom. The frequency of cells within a population undergoing this division event was estimated, and the cell division rate was calculated. In laboratory cultures, these rates of cell division and those calculated from changes in cell numbers were similar. By dual labeling with 68Ge(OH)4 and NaH14CO3, rates of cell division and photosynthesis were coincidently measured for diatoms both in laboratory cultures and when isolated from natural populations in estuarine, offshore, and polar environments. These techniques permit the coupling between photosynthesis and cell division to be examined in situ for individual species of diatom. 相似文献
11.
Histone H10 a differentiation-specific member of the histone H1 family, accumulates in cells during the terminal phase of cell differentiation, in tissues composed of arrested cells or cells exhibiting little proliferation. Moreover, the induction of cell proliferation in vivo, i.e., after partial hepatectomy, is accompanied by a decrease in H10 content. These observations suggest that H10 may be involved in the arrest of cell proliferation in vivo. In order to investigate this possibility, we took advantage of the fact that after partial hepatectomy the initiation of cell division is not synchronous. The strategy was to know, at the level of a single cell, whether H10 decreases prior to the initiation of the S phase or whether a cell can initiate DNA replication having a significant amount of H10 in the nucleus. We defined new protocols to analyze H10 content and cell proliferation at the level of a single cell, both in situ and by flow cytometry. The simultaneous determination of the relative amount of H10 and the position of cells in the cell cycle showed that no significant difference in H10 content was detected in cells actively replicating their DNA compared to nondividing cells. These observations have been confirmed by the successive immunodetections of H10 and BrdU in situ on the same cells. Therefore, we show here that in vivo, cells can initiate DNA replication with significant amounts of H10 and that the decrease of H10 is not a prerequisite of cell division. We propose that the accumulation of H10 is not related to the arrest of cell proliferation, but is controlled in such a manner that the protein accumulates in slowly dividing cells and decreases in rapidly growing cells. 相似文献
12.
SYNOPSIS. Temperature-induced patterns of synchronous cell division and cell size were obtained with Euglena gracilis. The alga was cultured in a glutamate-sucrose medium in 6-liter quantities. Synchrony was induced by non-lethal shifts of temperature between 14.5 and 28.5 C. Three liters of cells containing 2 × 106 cells/ml were harvested in each 24-hour cycle. 相似文献
13.
This paper couples a general d-dimensional (d arbitrary) model for the intracellular biochemistry of a generic cell with a probabilistic division hypothesis and examines
the consequence of division for stability of cell function and structure. We show rather surprisingly that cell division is
capable of giving rise to a stable population of cells with respect to function and structure even if, in the absence of cell
division, the underlying biochemical dynamics are unstable. In the context of a simple example, our stability condition suggests
that rapid cell proliferation plays a stabilizing role for cellular populations.
Received: 15 January 1996 / Revised version: 31 July 1998 相似文献
14.
The ocular circadian rhythm in the eye of Bulla gouldiana is generated by a rhythm in membrane potential of retinal neurons that is driven by alterations in potassium conductance. Since potassium conductance may be modulated by the phosphorylation of potassium channels, the circadian rhythm may reflect rhythmic changes in protein kinase activity. Furthermore, the circadian rhythm recorded from the Bulla eye can be phase shifted by agents that affect protein synthesis and protein phosphorylation on tyrosine residues. Interestingly, the eukaryotic cell division residues. Interestingly, the eukaryotic cell division cycle is generated by similar processes. Rhythmic cell division is regulated by periodic synthesis and degradation of a protein, cyclin, and periodic tyrosine phosphorylation of a cyclin-dependent kinase (cdk), p34cdc2. The interaction between these two proteins results in rhythmic kinase activity of p34cdc2. Both cyclin and p34cdc2 are pat of two diverse gene families, some of whose members have been localized to postmitotic cell types with no function yet determined. In the current work, we identify proteins similar to the cdks and cyclin in the eye of Bulla. Neither of these ocular proteins are found in mitotic cells in Bulla, and the cdk-like protein (p40) is specific to the eye. Furthermore, the concentration of the cyclin-like protein (p66) is affected by treatments that phase shift the circadain rhythm. The identification of cdk and cyclin-like proteins in the Bulla eye is consistent with the hypothesis that the biochemical mechanism responsible for generating the ocular circadian rhythm in Bulla is related to the biochemical mechnism that regulates the eukaryotic cell division cycle. 1994 John Wiley & Sons, Inc. 相似文献
15.
Lingbo Kong Rui Ma Yang Cao Wanli Smith Yuan Liu Xiaobin Yang Liang Yan 《Journal of cellular and molecular medicine》2021,25(10):4649-4657
Although document studies (including ours) have been reported the achieved in vitro osteoclastic cellular model establishment from the RAW264.7 cell lineage, there was no study directly reported that American Type Culture Collection (ATCC) cell bank has various RAW264.7 cell lineages. Besides that, for our knowledge there was only one study compared the two different RAW264.7TIB-71 and RAW264.7CRL-2278 cell lineages for their osteoclastic differentiation, and they concluded that the RAW264.7CRL-2278 demonstrated to generate much osteoclast than RAW264.7TIB-71. However, on the contrary to their results we noticed the fusion of RAW264.7TIB-71 in our previous studies was much compromising. Therefore, we try to explore the two cell lineages for their properties in osteoclastic differentiation with an in-depth cellular cytoskeletal study. Our current study has showed that comparing to the RAW264.7CRL-2278, RAW264.7TIB-71 demonstrated a much higher efficacies for RANKL-stimulated osteoclastic differentiation. Besides that, in our depth cytoskeletal studies, we found that the RANKL-induced RAW264.7TIB-71 cells could finally differentiate into mature osteoclasts. However, regardless the various pre-treatment conditions, there was no mature osteoclast formed in RANKL-induced RAW264.7CRL-2278 cell lineage. 相似文献
16.
Cell division and cell enlargement during potato tuber formation 总被引:14,自引:0,他引:14
Cell division and cell enlargement were studied to reveal the developmental
mechanism of potato tuberization using both in vivo
in vitro culture systems. Distribution of cells in
S-phase was visualized by immunolabelling of incorporated bromodeoxyuridine
(BrdU). Mitosis was detected in DAPI (4,6-di-amidino-2-phenylindole) or
toluidine blue-stained sections. Timing and frequency of cell division were
determined by daily cell counting, and cell enlargement was deduced from
measurements of cell diameters.Under in vivo
conditions, lateral underground buds developed into stolons due to
transverse cell divisions and cell elongation in the apical region of the
buds. At the onset of tuber formation, the elongation of stolons stopped
and cells in pith and cortex enlarged and divided longitudinally, resulting
in the swelling of the stolon tip. When tubers had a diameter of 0.8 cm,
longitudinal divisions had stopped but randomly oriented division and cell
enlargement occurred in the perimedullary region and continued until tubers
reached their final diameter.In vitro tubers were
formed by axillary buds on single node cuttings cultured under
tuber-including conditions. They stopped growing at a diameter of 0.8 cm.
Pith and cortex were involved in tuberization such as that found during the
early stage of in vivo tuberization (<0.8 cm in
diameter). The larger size of in vivo tubers is,
however, due to further development of the perimedullary region, which is
lacking in vitro conditions.Keywords:
Cell division, cell enlargement, DNA synthesis, in
vitro culture, potato, tuber formation.
相似文献
17.
Turnover of cell wall components was examined in two growth phases of a batch suspension culture of Vinca rosea L. Three-day-cultured cells (cell division phase) and 5-day-cultured cells (cell expansion phase) were incubated with d -[U-14C]glucose. After various periods of incubation, extra-cellular polysaccharides (ECP) and cell walls were isolated, and then the cell walls were fractionated to pectic substance, hemicellulose, and cellulose fractions. The results of the measurement of radioactivities and amounts of total carbohydrate in the ECP and cell wall fractions indicated that synthesis of pectic substance was more active in the cell division phase than in the cell expansion phase. From the results of the pulse-chase experiments, in which cells prelabelled by incubation with d -[U-14C]glucose for 3 h were incubated in a medium containing unlabelled glucose for various periods, the gross degradation, net synthesis, and gross synthesis of cell wall components were estimated. Active degradation and synthesis were observed in the hemicellulose fraction, indicating that active turnover occurred in the hemicellulose fraction, while little degradation was found in the pectic substance and cellulose fractions. 相似文献
18.
Capiod T 《Biochimie》2011,93(12):2075-2079
Both increases in the basal cytosolic calcium concentration ([Ca2+]cyt) and [Ca2+]cyt transients play major roles in cell cycle progression, cell proliferation and division. Calcium transients are observed at various stages of cell cycle and more specifically during late G1 phase, before and during mitosis. These calcium transients are mainly due to calcium release and reuptake by the endoplasmic reticulum (ER) and are observed over periods of hours in oocytes and mammalian cells. Calcium entry sustains the ER Ca2+ load and thereby helps to maintain these calcium transients for such a long period. Calcium influx also controls cell growth and proliferation in several cell types. Various calcium channels are involved in this process and the tight relation between the expression and activity of cyclins and calcium channels also suggests that calcium entry may be needed only at particular stages of the cell cycle. Consistent with this idea, the expression of l-type and T-type calcium channels and SOCE amplitude fluctuate along the cell cycle. But, as calcium influx regulates several other transduction pathways, the presence of a specific connection to trigger activation of proliferation and cell division in mammalian cells will be discussed in this review. 相似文献
19.
DONALD B. WILLIAMS 《The Journal of eukaryotic microbiology》1968,15(4):811-816
SYNOPSIS. Spathidium spathula is very sensitive to division inhibition after X-irradiation. Five kr delivered to animals 1 hr into the cell cycle prolong the period until the next division to about 2 times the normal length. The next 2 cell cycles, however, are shorter than normal, and by the 4th division irradiated cells have recovered the normal division rate. During this division delay, scanning interference microscopy shows that growth in dry mass continues; at the 1st post-irradiation division the cells average 3 times the normal dry mass. After the 2nd post-irradiation division, dry mass is 1.5 times the normal amount. Dry mass measurements were not made beyond the 2nd division. Giant cells produced by X-rays have enlarged macronuclei, indicating that DNA synthesis is not inhibited by a dose of X-rays that blocks division. Mitomycin C and triethylene melamine, agents which attack or damage DNA, also produce division blockage and giantism in Spathidium. This suggests that damage to DNA in either the macronucleus, the micronucleus or other organelles may be much more effective in delaying cell division than cell growth. 相似文献
20.
STEVEN W. HEWETT 《The Journal of eukaryotic microbiology》1980,27(3):311-313
SYNOPSIS. the cell size of Didinium nasutum was found to be dependent on the size of the Paramecium species available as prey. Didinium feeding on P. tetraurelia averaged 5.6 × 105μm3. the cell volume of Didinium increased with increasing prey size for the 5 prey species tested, to 9.1 × 105μm3 for Didinium feeding on P. caudatum. Didinium nearing a cell division ranged in size from 8.6 × 105μm3 on P. tetraurelia to 12.9 × 105μm3 on P. caudatum. the range in cell volume is such that Didinium feeding on P. caudatum are larger than the size at which Didinium divide when feeding on P. tetraurelia. This morphologic plasticity in cell volume allows Didinium to exploit a wide size range of Paramecium species as prey. It is proposed that the size of a Didinium may have profound effects on its ability to encounter and capture prey of different sizes. 相似文献