Toxicities effects of pharmaceutical, olive mill and textile wastewaters before and after degradation by Pseudomonas putida mt-2

Background  

Removal of numerous classes of chemical pollutants from the industrial wastewater such as textile, pharmaceutical and olive mill using conventional wastewater treatment, is incomplete and several studies suggested that improvement of this situation would require the application of biological treatment techniques. Dyes, polyphenols and drugs are an environmental pollutants extremely toxics to plants and other living organisms including humans. These effluents were previously treated by Pseudomonas putida. The main of this work was to evaluate the in vivo toxicity of the three wastewaters.     

Environmental impact assessment of centralized municipal wastewater management in Thailand

Purpose

Urbanization and industrial development intensify water utilization and wastewater generation. The efficiency of wastewater treatment systems varies and depends on system design and wastewater condition. The research aims to examine seven existing centralized municipal wastewater treatment plants (WWTPs) in Bangkok to discover which system configuration yields the best environmental and economic performance. The degree of environmental impact and operational costs from different system designs were investigated to help select future wastewater treatment systems.

Methods

Life cycle assessment (LCA) has been conducted to evaluate environmental impacts from centralized municipal wastewater treatment systems. Life cycle impact assessment method based on endpoint modeling (LIME) was applied, with three major potential environmental impact categories including eutrophication, global warming, and acidification. All seven centralized municipal WWTPs in Bangkok were investigated as case studies. The system configurations are classified into five types of activated sludge (AS) systems. The contribution of impacts from individual processes in each type of AS system was analyzed. The methodology covered major on-site and off-site operational processes excluding construction and maintenance phases. Average annual data were calculated to develop an inventory dataset. JEMAI-Pro software was utilized in this study to analyze the life cycle impact of the systems.

Results and discussion

The level of environmental impact from a WWTP depends on the configuration of the AS system. The highest potential environmental impact from a municipal WWTP is eutrophication, which is obviously affected by ammonium and phosphorous discharges into water bodies. The vertical loop reactor activated sludge (VLRAS) system yielded the best treatment performance among the five AS sub-systems. The consumption of electricity used to operate the system contributed significantly to global warming potential and correlated considerably with operating costs. Comparing among three system sizes, the large-scale WWTP revealed inefficient electricity consumption, whereas the medium plant provided better performance in chemical use and operating costs.

Conclusions

Centralized municipal WWTPs with capacities ranging from 10 to 350?×?10³ m³/day were evaluated with respect to environmental performance and costs during the operating phase. Among all case studies, a medium-scale WWTP with a VLRAS system offered the best operating performance in terms of low environmental impact, resource consumption, and cost. To enhance WWTP management, it is vital to improve the efficiency of electricity consumption in primary and secondary treatment processes and increase wastewater collection efficiency to maximize the plant operating capacity and minimize overall environmental impacts.

     

Prospective CO<Subscript>2</Subscript> emissions from energy supplying systems: photovoltaic systems and conventional grid within Spanish frame conditions

Background, aim, and scope  

In order to assess the environmental sustainability of a novel wastewater treatment process based on power an electrochemical reactor by photovoltaic solar modules (photovoltaic solar electrochemical oxidation), a life cycle approach was considered to quantify the CO₂ equivalent (CO₂-eq.) emissions coming from the two supplying power systems to the electrochemical process: conventional grid power or photovoltaic solar power under Spain frame conditions.     

Environmental assessment of municipal wastewater discharges: a comparative study of evaluation methods

Purpose

Wastewater treatment plants produce high environmental impacts on receiving water bodies and pose economic burdens on municipalities or industrial facilities. Their overall operational costs and achieved effluent quality depend very much on the influent type, presence of priority pollutants, treatment technology and required effluent quality (for discharge or for recycle/reuse). Life cycle assessment (LCA), environmental impact quantification (EIQ) and water footprint (WF) are important instruments for sustainability assessments applied for products, production and consumption evaluations in connection with natural resources depletion and pollution threats. This study focuses on the environmental assessment of a municipal wastewater treatment plant (MWWTP) discharges by means of these three evaluation methods with the purpose to understand their (methodological) weak and strong points in capturing the impacts. Such a comparative analysis is necessary to understand how the (individual) advantages provided by each of these instruments can be complimentarily used to improve an assessment framework for various stakeholders concerned with water use cycle management (regional water operators, water management authority, public authorities, research entities, societal organizations, etc.).

Methods

The three assessment methodologies (LCA, EIQ, WF) are presented, implemented and critically analysed based on a unitary set of data concerning the MWWTP of Iasi city (a municipality of approx. 300,000 inhabitants situated in the North Eastern region of Romania), the wastewater and river water quality indicators as well as all the other relevant data being collected for the year 2012.

Results and discussion

Although the three methodologies have different principles for environmental impact quantification, the results have shown that most impacts induced to surface waters due to Iasi MWWTP effluents are given by the nutrients (nitrogen and phosphorous compounds), which could induce an eutrophication impact, and to a lesser extent by pollutants responsible for toxicity impacts (such as heavy metals).

Conclusions

Based on the results of this comparative study, a critical analysis of these three methods was realized by considering the data requirements, their development and integration status. Furthermore, the strong and weak points that are relevant for each method implementation and their subsequent use by decision-makers and Water Authorities are discussed, in the context of legislative requirements (including the Water Framework Directive), actual development of regional water operators and stakeholders' interests.

     

A comparative life cycle assessment of two treatment technologies for the Grey Lanaset G textile dye: biodegradation by Trametes versicolor and granular activated carbon adsorption

Purpose

The aim of this study is to use life cycle assessment (LCA) to compare the relative environmental performance of the treatment using Trametes versicolor with a common method such as activated carbon adsorption. This comparison will evaluate potential environmental impacts of the two processes. This work compiles life cycle inventory data for a biological process that may be useful for other emergent biotechnological processes in water and waste management. LCA was performed to evaluate the use of a new technology for the removal of a model metal-complex dye, Grey Lanaset G, from textile wastewater by means of the fungus T. versicolor. This biological treatment was compared with a conventional coal-based activated carbon adsorption treatment to determine which alternative is preferable from an environmental point of view.

Materials and methods

The study is based on experimental research that has tested the novel process at the pilot scale. The analysis of the biological system ranges from the production of the electricity and ingredients required for the growth of the fungus and ends with the composting of the residual biomass from the process. The analysis of the activated carbon system includes the production of the adsorbent material and the electricity needed for the treatment and regeneration of the spent activated carbon. Seven indicators that measure the environmental performance of these technologies are included in the LCA. The indicators used are climate change, ozone depletion, human toxicity, photochemical oxidant formation, terrestial acidification, freshwater eutrophication, marine eutrophication, terrestrial ecotoxicity, freshwater ecotoxicity, marine ecotoxicity, metal depletion and fossil depletion.

Results

The results show that the energy use throughout the biological process, mainly for sterilisation and aeration, accounts for the major environmental impacts with the inoculum sterilisation being the most critical determinant. Nevertheless, the biological treatment has lower impacts than the physicochemical system in six of these indicators when steam is generated directly on site. A low-grade carbon source as an alternative to glucose might contribute to reduce the eutrophication impact of this process.

Conclusions

The LCA shows that the biological treatment process using the fungus T. versicolor to remove Grey Lanaset G offers important environmental advantages in comparison with the traditional activated carbon adsorption method. This study also provides environmental data and an indication of the potential impacts of characteristic processes that may be of interest for other applications in the field of biological waste treatment and wastewater treatment involving white-rot fungi.     

Evaluation of water services system through LCA. A case study for Iasi City,Romania

Purpose

The main objective of this paper is to analyse through life cycle assessment (LCA), the entire water services system in Iasi City (Romania): a representative city for the problems faced by the water services sector in Romania. Furthermore, the study is aimed at demonstrating the usefulness of the LCA approach as a support instrument for water resources management.

Methods

The life cycle inventory (LCI) of the Iasi water system was organized considering the water system components, as well as their function related to the water use life cycle: before the tap system as production phase (water abstraction, transport, treatment and distribution) and after the tap section as post-use phase (wastewater collection, treatment and discharge). The foreground data describing the LCI processes were provided directly by the company operating the Iasi water system, while the data for the background processes were sourced or selected from Ecoinvent 2.0 database. The assessment considers the quantification of environmental impacts (according to the CML 2000 baseline and Ecological Scarcity 2006 methodologies) of water supply (abstraction, treatment and distribution) and wastewater disposal (collection and treatment) relative to 1 m³ of tap water.

Results and discussion

For this given system, the results have pointed out that the before the tap system generates higher impacts than the after tap system, mainly due to the energetic effort needed for water supply and the fairly high water losses in the distribution system. However, the after the tap system, specifically the discharge of treated wastewater is still responsible for many of the water-related impact such as Eutrophication (when using CML) or Emissions to surface waters (when using the Ecological Scarcity method). Apart from the LCA approach, this study presents several scenarios for the improvement of the environmental performance of the water services, such as: changing between water sources, improving the distribution system and upgrading the wastewater treatment plant.

Conclusions

This study has demonstrated the usefulness of LCA to describe, compare and predict the environmental performance of complex water services systems (and all its components). The results have provided a reference case for the environmental profile of Iasi city water system, and have enabled the identification of its improvement alternatives. Also, this study, which represents a premiere for Romania, has opened future research directions which may include the development perspectives of the Iasi water services system, as well as improvements of LCIA methodologies to better represent the local specific water-related impacts.     

Life cycle inventory practices for major nitrogen,phosphorus and carbon flows in wastewater and sludge management systems

Purpose

Nitrogen, phosphorus and carbon originating from wastewater and sludge can, depending on their partitioning during wastewater treatment, either become available as potential resources or leave as emissions. Several reviews have highlighted the dependence of life cycle assessment (LCA) results on the inventory data. To provide a foundation for future assessments of systems in which resources are utilised from wastewater or sludge, this paper identifies common practice and highlights deficiencies in the selection and quantification of nitrogen, phosphorus and carbon containing flows.

Methods

Inventories of major direct flows containing nitrogen, phosphorus and carbon in 62 studies on wastewater and sludge management operations have been reviewed. A special focus was put on flows of nitrogen, phosphorus and carbon originating from the wastewater and sludge and on how these are either leaving the system as emissions and hereby contributing to environmental impacts, or how potential resource flows of these elements are accounted for, in particular when sludge is used in agriculture.

Results and discussion

The current study shows a large variation between studies regarding what resource and emission flows were included in inventories on wastewater and sludge treatment, the type of data used (primary or secondary data) and, when flows have been modelled rather than measured, how the modelling has been done. Except for nitrogen and phosphorus emissions via the effluent, which were generally quantified using measured data or data modelled to represent the specific situation, direct emissions to air from the water and sludge lines at the wastewater treatment plant were mostly estimated using secondary data, sometimes of poor data quality. In systems where resources were recovered through agricultural application of sludge, studies often credited the system for avoided use of mineral fertiliser, but the considered replacement ratio differed.

Conclusions

The current review identified increased completeness and specificity in the modelling of the evaluated flows as particularly relevant for future studies and highlighted a need for improved transparency of data inventories. The review can be used as a support for LCA analysts in future studies, providing an inventory of common practices and pinpointing deficiencies, and can thereby support more conscious and well-motivated choices as regard which flows to include in assessments and on the quantification of these flows.

     

Isolation and characterisation of polychlorinated biphenyl (PCB) degrading fungi from a historically contaminated soil

Background  

Polychlorinated biphenyls (PCBs) are widespread toxic pollutants. Bioremediation might be an effective, cost competitive and environment-friendly solution for remediating environmental matrices contaminated by PCBs but it is still unsatisfactory, mostly for the limited biodegradation potential of bacteria involved in the processes. Very little is known about mitosporic fungi potential in PCB bioremediation and their occurrence in actual site historically contaminated soils. In the present study, we characterised the native mycoflora of an aged dump site soil contaminated by about 0.9 g kg^-1 of Aroclor 1260 PCBs and its changing after aerobic biotreatment with a commercial complex source of bacteria and fungi. Fungi isolated from the soil resulting from 120 days of treatment were screened for their ability to adsorb or metabolise 3 target PCBs.     

Occurrence and reduction of human viruses,F‐specific RNA coliphage genogroups and microbial indicators at a full‐scale wastewater treatment plant in Japan

Aims

To evaluate and compare the reductions of human viruses and F‐specific coliphages in a full‐scale wastewater treatment plant based on the quantitative PCR (qPCR) and plate count assays.

Methods and Results

A total of 24 water samples were collected from four locations at the plant, and the relative abundance of human viruses and F‐RNA phage genogroups were determined by qPCR. Of the 10 types of viruses tested, enteric adenoviruses were the most prevalent in both influent and effluent wastewater samples. Of the different treatment steps, the activated sludge process was most effective in reducing the microbial loads. Viruses and F‐RNA phages showed variable reduction; among them, GI and GIII F‐RNA phages showed the lowest and the highest reduction, respectively.

Conclusions

Ten types of viruses were present in wastewater that is discharged into public water bodies after treatment. The variability in reduction for the different virus types demonstrates that selection of adequate viral indicators is important for evaluating the efficacy of wastewater treatment and ensuring the water safety.

Significance and Impact of the Study

Our comprehensive analyses of the occurrence and reduction of viruses and indicators can contribute to the future establishment of appropriate viral indicators to evaluate the efficacy of wastewater treatment.     

Synthesis and characterization of core-shell Fe<Subscript>3</Subscript>O<Subscript>4</Subscript>-gold-chitosan nanostructure

Background  

Fe₃O₄-gold-chitosan core-shell nanostructure can be used in biotechnological and biomedical applications such as magnetic bioseparation, water and wastewater treatment, biodetection and bioimaging, drug delivery, and cancer treatment.     

Biological treatment of tannery wastewater by using salt-tolerant bacterial strains

Background  

High salinity (1–10% w/v) of tannery wastewater makes it difficult to be treated by conventional biological treatment. Salt tolerant microbes can adapt to these saline conditions and degrade the organics in saline wastewater.     

Smoking and nicotine exposure delay development of collagen-induced arthritis in mice

Introduction  

Recent epidemiologic studies have implicated smoking as an environmental risk factor for the development of rheumatoid arthritis (RA). The aim of the present study is the evaluation of the role of cigarette smoke (CS) in the pathogenesis of collagen-induced arthritis in mice.     

Past vegetation dynamics in the Yellowstone region highlight the vulnerability of mountain systems to climate change

Aim

Reconstruct the long‐term ecosystem dynamics of the region across an elevational gradient as they relate to climate and local controls. In particular, we (1) describe the dominant conifers' history; (2) assess changes in vegetation composition and distribution; and (3) note periods of abrupt change versus stability as means of better understanding vegetation responses to environmental variability.

Location

Greater Yellowstone Ecosystem (GYE; USA).

Time period

16.5 ka bp ‐present.

Major taxa studied

Juniperus, Picea, Abies, Pinus, Pseudotsuga.

Methods

The vegetation reconstruction was developed from 15 pollen records. Results were interpreted based on modern pollen–vegetation relationships estimated from a suite of regression‐based approaches.

Results

Calibrated pollen data suggest that late‐glacial vegetation, dominated by shrubs and Juniperus, lacks a modern counterpart in the area. Picea, Abies and Pinus expanded at 16 ka bp in association with postglacial warming and co‐occurred in mixed‐conifer parkland/forest after 12 ka bp . This association along with Pinus contorta forest, which was present after 9 ka bp , has persisted with little change at middle and high elevations to the present day. This stability contrasts with the dynamic history of plant communities at low elevations, where shifts between parkland, steppe and forest over the last 8,000 years were likely driven by variations in effective moisture and fire.

Main conclusions

The postglacial vegetation history of the GYE highlights the dynamic nature of mountain ecosystems and informs on their vulnerability to future climate change: (1) most of the conifers have been present in the area for >12,000 years and survived climate change by adjusting their elevational ranges; (2) some plant associations have exhibited stability over millennia as a result of nonclimatic controls; and (3) present‐day forest cover is elevationally more compressed than at any time in history, probably due to the legacy of the Medieval Climate Anomaly and the Little Ice Age.     

Verifying the effectiveness of environmental performance improvement actions in the chain of production of an agrochemical produced in Brazil

Purpose

A Brazilian agrochemical company agreed to conduct an initiative to further evaluate the environmental impact caused by its product SC50. This agrochemical is obtained from thiophanate-methyl, an active ingredient produced in Brazil as well as in Japan, where another industrial plant of the same corporation is located. The initiative evaluated the environmental performance of the SC50 life cycle so as to provide the company’s private management with information to influence stakeholders in the sector.

Methods

The working method comprised five steps. Step 1 established the impact profile associated to the SC50 life cycle. The diagnosis was obtained by LCA from a ‘cradle-to-grave’ approach. Step 2 identified the stages causing significant environmental impacts throughout the entire life cycle. In Step 3, improvement actions were proposed in order to mitigate, reduce, or even minimize the effects detected. Step 4 comprised the modeling, in which specific scenarios and their environmental impacts were analyzed. The synergistic effect was checked by successive additions of improvement actions, characterizing each scenario. Step 5 analyzed the results, comparing impact profiles of each scenario with the original diagnosis (as a baseline scenario) and verifying the individual effect of each action.

Results and discussion

The results indicate relevant contributions of the dispersion from the SC50 life cycle in terms of global warming, terrestrial ecotoxicity, human toxicity, and eutrophication. Regarding to the manufacture, the use of diesel has great influence in the impacts of SC50, and its performance as eutrophication is conditioned to the low efficiency of the wastewater treatment. While the company decided not to implement improvements in the dispersion stage fearing market losses, five alternatives based on cleaner production principles were proposed to improve performance: to review the instrumentation systems in the plant, to adjust wastewater treatment, to stop importing thiophanate-methyl from Japan, to install an energy cogeneration system, and to substitute renewable glycerin with a fossil counterpart. All scenarios led to improvements from baseline.

Conclusions

The use of LCA determined the impact profile associated to SC50 in soybean pest control. Because of strategic reasons, the company decided not to propose improvements in the most significant stage of this life cycle. Among the improvements, we highlight the replacement of imported thiophanate-methyl by a Brazilian equivalent and the installation of a combined cycle for energy recovery. For both these cases, however, the appropriate organizational measures must be taken before implementation.

     

The Water Impact Index: a simplified single-indicator approach for water footprinting

Purpose

Along with climate change-related issues, improved water management is recognized as one of the major challenges to sustainability. However, there are still no commonly accepted methods for measuring sustainability of water uses, resulting in a recent proliferation of water footprint methodologies. The Water Impact Index presented in this paper aims to integrate the issues of volume, scarcity and quality into a single indicator to assess the reduction of available water for the environment induced by freshwater uses for human activities.

Methods

The Water Impact Index follows life cycle thinking principles. For each unit process, a volumetric water balance is performed; water flows crossing the boundaries between the techno-sphere and environment are multiplied by a water quality index and a water scarcity index. The methodology is illustrated on the current municipal wastewater management system of Milan (Italy). The Water Impact Index is combined with carbon footprint to introduce multi-impact thinking to decision makers. The Water Impact Index is further compared to results obtained using a set of three life cycle impact indicators related to water, from the ReCiPe life cycle impact assessment (LCIA) methodology.

Results and discussion

Onsite water use is the main contribution to the Water Impact Index for both wastewater management schemes. The release of better quality water is the main driver in favour of the scenario including a wastewater treatment plant, while the energy and chemicals consumed for the treatment increase the indirect water footprint and carbon footprint. Results obtained with the three midpoint indicators depict similar tendencies to the Water Impact Index.

Conclusions

This paper presents a simplified single-indicator approach for water footprinting, integrating volume, scarcity and quality issues, representing an initial step toward a better understanding and assessment of the environmental impacts of human activities on water resources. The wastewater treatment plant reduces the Water Impact Index of the wastewater management system. These results are consistent with the profile of the three midpoint indicators related to water from ReCiPe.     

Comparison of the environmental impact of the conventional nickel electroplating and the new nickel electroplating

Purpose

To comply with the effluent regulation of boron, replacement of boric acid with citric acid in a nickel electroplating bath is proposed. Although the bath avoids the discharge of boron, it increases the discharge of nickel owing to the chelating effect of citric acid, which disturbs the wastewater treatment. To balance this trade-off, the environmental impacts of a traditional nickel plating process (the Watts bath) and the citrate bath must be compared by life cycle assessment.

Methods

The life cycle impact assessment method was LIME2. To estimate the trade-off between boron and nickel discharge into wastewater, the characterization and damage factors on human toxicity and ecotoxicity were calculated. The processes were then compared using data from actual processes. The functional unit was “plating per 1-kg part.” However, the plating efficiency depends on the type, shape, and surface area of the part. The data of the citrate bath were modeled. In the modeling, the amounts of nickel chloride and nickel sulfate in the citrate bath were based on the Watts bath.

Results and discussion

In comparison with other chemicals, the calculated characterization and damage factors of boron and nickel were found to be reasonable. The integration results revealed that the citrate bath exerted greater environmental impact than the Watts bath. Although the Watts bath involved more environmentally damaging processes than the citrate bath, the sum of these impacts was much smaller than the impact of effluent from the citrate bath. Moreover, the environmental impact of effluent can be significantly reduced by flocculants, with almost no additional environmental impact incurred by the increased sludge.

Conclusions

The newly developed citrate plating bath exerts higher environmental impact than the traditional Watts bath because the environmental impacts of the release of nickel chelated with citric acid exceed the reduced boron emissions. Therefore, there is a trade-off between the two methods. When installing the citrate bath, the wastewater treatment must be altered to reduce the nickel emissions.

     

A review of environmental life cycle assessment studies examining cheese production

Purpose

Cheese is one of the world’s most widely consumed dairy products and its popularity is ever growing. However, as concerns for the environmental impact of industries increase, products like cheese, which have a significant environmental impact, may lose their popularity. A commonly used technique to assess the environmental impact of a product is life cycle assessment (LCA). In this paper, a state-of-the-art review of LCA studies on the environmental impact of cheese production is presented.

Methods

Sixteen LCA studies, which explored the impact from the production of a variety of cheese types (fresh, mature and semi-hard) were examined and discussed. The four stages of the LCA were examined and the range of results of selected environmental impact categories (global warming potential, acidification potential and eutrophication potential) were detailed and discussed.

Results and discussion

For each of these environmental impact categories, raw milk production was consistently found to be the most significant contributor to the total impact, which was followed by processing. It was found that allocation between cheese and its by-products was crucial in determining the impact of cheese production and standardisation or guidelines may be needed. Very little information relating to wastewater treatment system and processes were reported and this leads to inaccurate environmental impact modelling relating to these aspects of the manufacture of cheese. Very few studies included the design of packaging in terms of reducing food waste, which may significantly contribute to the overall environmental impact.

Conclusions

As raw milk production was found to have the greatest contribution to environmental impact, mitigation strategies at farm-level, particularly in relation to enteric fermentation and manure management, need to be implemented. Additionally, based on the literature, there is a suggestion that fresh cheese has less of an environmental impact than semi-hard cheeses, particularly when examining direct energy consumption. However, there needs to be more case studies investigated to justify this statement.

     

Environmental performance of a municipal wastewater treatment plant

Goal, Scope and Background  Nowadays, every strategy must be developed taking into account the global impact on the environment; if this aspect is forgotten, a change of environmental loads or their effect will be caused and no reduction will be attained. For instance, a wastewater treatment plant (WWIP), which is considereda priori as an ecological treatment system, gives rise to an environmental impact due to its energy consumption, use of chemical compounds, emissions to the atmosphere and sludge production, the post-treatment of which will also have diverse environmental effects. The goal of this study is to evaluate the potential environmental impact corresponding to a municipal WW1P and to identify the hot spots associated with the process. Methods  In this study, the Centre of Environmental Science (CML) of Leiden University methodology has been considered to quantify the potential environmental impact associated with the system under study. A comprehensive analysis of the WWTP was evaluated for the physico-chemical characterisation of the wastewaters as well as the inventory of all the inputs (energy, chemical compounds, ...) and outputs (emissions to air, water, soil and solid waste generation) associated with the global process. Regarding Life Cycle Inventory Assessment, SimaPro 5.0 was used and in particular CML factors (updated in 2002) were chosen for characterisation and normalisation stages. Results and Discussion  A comprehensive inventory of empirical data from water, sludge and gas flows during 2000 and 2001 was obtained. Two impact categories arise due to their significance: eutrophication and terrestrial ecotoxicity. Consequently, the aspects to be minimised in order to reduce the environmental impact of the system are the pollutant load at the watercourse discharge (mainly NH₃, PO₄ ^[3- and COD, even when all of them are below legal limits) and the emissions to soil (mainly Cr, Hg and Zn, even when they are present in low concentrations) when the sludge is used for agricultural application. Conclusions  As far as the environmental impact is concerned, differentiation between humid and dry season is not required as results are practically equal for both situations. Water discharge and sludge application to land have turned out to be the main contributors in the environmental performance of a WWTP. Regarding the former, the removal of nitrogen by means of a nitrification-denitrification system coupled to conventional biological aerobic treatment implies a high environmental impact reduction and, as for the latter, bearing in mind the proposed legislation, heavy metals as well as pathogens are supposed to be the key parameters to define the most adequate treatment strategies for the generated sludge. Recommendations and Outlook  This study can serve as a basis for future studies that can apply a similar policy to a great number of wastewater facilities. Besides, features such as different treatment systems and capacities can provide additional information with the final aim of including the environmental vector in the decision-making process when the operation of a WWTP is intended to be optimised. Moreover, sludge must also be a focus of attention due to the expected increase and its major contribution to the global environmental impact of a WWTP, which can determine other treatment alternatives.     

Environmental amoebae do not support the long‐term survival of virulent mycobacteria

Aims

To test the hypothesis that Mycobacterium bovis can persist in the environment within protozoa.

Methods and Results

In this study, we used a novel approach to detect internalized mycobacteria in environmental protozoa from badger latrines. Acid‐fast micro‐organisms were visualized in isolated amoebae, although we were unable to identify them to species level as no mycobacteria were grown from these samples nor was M. bovis detected by IS6110 PCR. Co‐incubation of Acanthamoeba castellanii with virulent M. bovis substantially reduced levels of bacilli, indicating that the amoebae have a negative effect on the persistence of M. bovis.

Conclusions

The internalization of mycobacteria in protozoa might be a rare event under environmental conditions. The results suggest that amoebae might contribute to the inactivation of M. bovis rather than representing a potential environmental reservoir.

Significance and Impact of the Study

Protozoa have been suggested to act as an environmental reservoir for M. bovis. The current study suggests that environmental amoebae play at most a minor role as potential reservoirs of M. bovis and that protozoa might inhibit persistence of M. bovis in the environment.