共查询到20条相似文献,搜索用时 31 毫秒
1.
A. Castillo H. Budak A.C. Martín G. Dorado A. Börner M. Röder P. Hernandez 《The Annals of applied biology》2010,156(3):347-356
A selection of 147 wheat D-genome and 130 barley genomic simple sequence repeat (gSSR) markers were screened for their utility in Hordeum chilense, as an alien donor genome for cereal breeding. Fifty-eight wheat D-genome and 71 barley PCR primer pairs consistently amplified products from H. chilense. Nineteen wheat D-genome and 20 barley gSSR markers were polymorphic and allowed wide genome coverage of the H. chilense genome. Twenty-three of the wheat D-genome and 11 barley PCR primer pairs were suitable for studying the introgressions of H. chilense into wheat, amplifying H. chilense products of distinct size. In 88% of the markers tested, H. chilense products were maintained in the expected homeologous linkage group, as revealed by the analysis of wheat/H. chilense addition lines. Twenty-nine microsatellite markers (eight gSSRs and 21 expressed sequence tags-SSRs) uniformly distributed across the genome were tested for their utility in genetic diversity analysis within the species. Three genetic clusters are reported, in accordance with previous morphological and amplified fragment length polymorphism data. These results show that it is possible to discriminate the three previously established germplasm groups with microsatellite markers. The reported markers represent a valuable resource for the genetic characterisation of H. chilense, for the analysis of its genetic variability, and as a tool for wheat introgression. This is the first intraspecific study in a collection of H. chilense germplasm using microsatellite markers. 相似文献
2.
P. Hernández G. Dorado P. Prieto M. J. Giménez M. C. Ramírez D. A. Laurie J. W. Snape A. Martín 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(8):1259-1264
The first genetic map of the wild South Ameri- can barley species Hordeum chilense is presented. The map, based on an F2 population of 114 plants, contains 123 markers, including 82 RAPDs, 13 SSRs, 16 RFLPs, four SCARs, two seed storage proteins
and two STS markers. The map spans 694 cM with an average distance of 5.7 cM between markers. Six additional SSRs and seven
additional SCARs which were not polymorphic were assigned to chromosomes using wheat/H. chilense addition lines. Polymorphisms were revealed by 50% of the RAPD amplifications, 13% of wheat and barley SSR primers, and 78%
of the Gramineae RFLP anchor probes. The utility of SSR and RFLP probes from other Gramineae species shows the usefulness
of a comparative approach as a source of markers and for aligning the genetic map of H. chilense with other species. This also indicates that the overall structure of the H. chilense linkage groups is probably similar to that of the B and D genomes of wheat and the H genome of barley. Applications of the
map for tritordeum and wheat breeding are discussed.
Received: 20 August 2000 / Accepted: 22 September 2000 相似文献
3.
Almudena Castillo Hikmet Budak Rajeev K Varshney Gabriel Dorado Andreas Graner Pilar Hernandez 《BMC plant biology》2008,8(1):1-9
Background
Hordeum chilense, a native South American diploid wild barley, is a potential source of useful genes for cereal breeding. The use of this wild species to increase genetic variation in cereals will be greatly facilitated by marker-assisted selection. Different economically feasible approaches have been undertaken for this wild species with limited direct agricultural use in a search for suitable and cost-effective markers. The availability of Expressed Sequence Tags (EST) derived microsatellites or simple sequence repeat (SSR) markers, commonly called as EST-SSRs, for barley (Hordeum vulgare) represents a promising source to increase the number of genetic markers available for the H. chilense genome.Results
All of the 82 barley EST-derived SSR primer pairs tested for transferability to H. chilense amplified products of correct size from this species. Of these 82 barley EST-SSRs, 21 (26%) showed polymorphism among H. chilense lines. Identified polymorphic markers were used to test the transferability and polymorphism in other Poaceae family species with the aim of establishing H. chilense phylogenetic relationships. Triticum aestivum-H. chilense addition lines allowed us to determine the chromosomal localizations of EST-SSR markers and confirm conservation of the linkage group.Conclusion
From the present study a set of 21 polymorphic EST-SSR markers have been identified to be useful for diversity analysis of H. chilense, related wild barleys like H. murinum, and for wheat marker-assisted introgression breeding. Across-genera transferability of the barley EST-SSR markers has allowed phylogenetic inference within the Triticeae complex. 相似文献4.
Cross-species amplification of the Hordeum chilense genome using barley sequence-tagged-sites (STSs)
A selection of 51 barley Sequence-Tagged Sites (STSs) were studied for their utility in Hordeum chilense. They included four primer sets from wheat origin and six primer sets from oat origin. Forty-four primer pairs amplified H. chilense products consistently. Five primer pairs were suitable for studying the introgression of H. chilense in wheat because they amplified H. chilense products of distinct size. Six of the STSs showed polymorphism between different H. chilense accessions. The results showed that barley STSs could be useful for the genetic characterization of H. chilense, tritordeums and derived introgression lines. 相似文献
5.
P. Hernández M. Hemmat N. F. Weeden G. Dorado A. Martín 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(5):721-727
RAPD markers were developed for octoploid×Tritordeum (amphiploid Hordeum chilense×Triticum aestivum) and its parents. Addition lines were used to identify specific RAPD markers for the Hordeum chilense chromosomes detectable in a wheat background. Twelve RAPD fragments have been cloned, sequenced and converted into STS markers.
Eleven of these STSs have maintained both the chromosome specificity and the possibility of detection in a wheat background.
The use of these markers in multiplexed PCRs facilitates both the efficient and reliable screening of new addition lines as
well as the monitoring of introgression of H. chilense in bread and durum wheat.
Received: 5 June 1998 / Accepted: 17 September 1998 相似文献
6.
C. Taylor K. Madsen S. Borg M. C. Møller B. Boelt P. H. Holm 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(4):648-658
Genetic analysis, particularly the development of genetic linkage maps in forage grass species, lags well behind other members
of the Poaceae. Comparative mapping within this family has revealed extensive conservation in gene and marker synteny among chromosomes
of diverse genera. Recently, the ability to transfer mapped STS markers between barley and wheat has been demonstrated. The
transfer of mapped STS markers between cereals and forage grasses could provide PCR-based markers for comparative mapping
in these species providing they amplify homologous sequences. In this study, primers derived from three barley genes of defined
function and a gene from Phalaris coerulescens were used to amplify homologous fragments in Lolium perenne. Primers derived from two barley and two oat cDNA clones were also tested along with eight barley and two Triticum tauchii STS markers. Twenty one primer pairs derived from 18 loci were tested. Eleven primer pairs (52%) amplified homologous sequences
in L. perenne from ten (55%) of the loci targetted. Thirteen new STS markers were generated in L. perenne, of which ten have been mapped in barley or rye and amplify homologous sequences in L. perenne.
Received: 20 October 2000 / Accepted: 13 January 2001 相似文献
7.
Barley (Hordeum vulgare L.) is potentially a new source of genes for wheat (Triticum aestivum L.) improvement. Wheat-barley chromosome recombinant lines provide a means for introgressing barley genes to wheat genome by chromosome engineering, and since these are expected to occur only rarely in special cytogenetic stocks, an efficient selection skill is necessary to identify them. To convert RFLP markers to barley allele-specific PCR markers useful for effective production of wheat-barley recombinant lines, 91 primer sets derived from RFLP clones which were previously mapped to the barley chromosomes were examined for PCR amplification using 'Chinese Spring' wheat, 'Betzes' barley and the wheat-barley chromosome addition lines. The polymorphisms were detected by an agarose gel electrophoresis of the PCR products without digestion with restriction enzymes. Out of 81 primer sets producing polymorphisms between the wheat and barley genomes, 26 amplified barley chromosome-specific DNAs which were confirmed to be located on the same chromosome as the RFLP markers by using the wheat-barley chromosome addition lines. These amplified DNAs represent barley allele-specific amplicons, which distinguish barley alleles from their wheat homoeologous counterparts. The present investigation revealed a higher probability for obtaining allele-specific amplicons from genomic DNA-derived RFLP markers than from cDNA-derived ones. The barley allele-specific amplicons developed in this study, namely, four for chromosome 2H, two for 3H, seven for 4H, eight for 5H, one for 6H and four for 7H, are suitable for identifying 'Chinese Spring' wheat- 'Betzes' barley recombinant chromosomes. However, one out of eight barley allele-specific amplicons on chromosome 5H did not detect a unique barley band in a 'New Golden' barley chromosome 5H addition line of 'Shinchunaga' wheat, indicating there may be a need to reconstruct allele-specific amplicons with different barley cultivars. 相似文献
8.
9.
X. Shan T. K. Blake L. E. Talbert 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1072-1078
Conversion of amplified fragment length polymorphisms (AFLPs) to sequence-specific PCR primers would be useful for many genetic-linkage
applications. We examined 21 wheat nullitetrasomic stocks and five wheat-barley addition lines using 12 and 14 AFLP primer
combinations, respectively. On average, 36.8% of the scored AFLP fragments in the wheat nullitetrasomic stocks and 22.3% in
the wheat-barley addition lines could be mapped to specific chromosomes, providing approximately 461 chromosome-specific AFLP
markers in the wheat nullitetrasomic stocks and 174 in the wheat-barley addition lines. Ten AFLP fragments specific to barley
chromosomes and 16 AFLP fragments specific to wheat 3BS and 4BS chromosome arms were isolated from the polyacrylamide gels,
re-amplified, cloned and sequenced. Primer sets were designed from these sequences. Amplification of wheat and barley genomic
DNA using the barley derived primers revealed that three primer sets amplified DNA from the expected chromosome, five amplified
fragments from all barley chromosomes but not from wheat, one amplified a similar-sized fragment from multiple barley chromosomes
and from wheat, and one gave no amplification. Amplification of wheat genomic DNA using the wheat-derived primer sets revealed
that three primer sets amplified a fragment from the expected chromosome, 11 primer sets amplified a similar-sized fragment
from multiple chromosomes, and two gave no amplification. These experiments indicate that polymorphisms identified by AFLP
are often not transferable to more sequence-specific PCR applications.
Received: 30 June 1998 / Accepted: 26 October 1998 相似文献
10.
A Genetic linkage map of Pinyon pine (Pinus edulis) based on amplified fragment length polymorphisms 总被引:1,自引:0,他引:1
S. E. Travis K. Ritland T. G. Whitham P. Keim 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):871-880
Amplified fragment length polymorphisms (AFLP) were used to rapidly generate a dense linkage map for pinyon pine (Pinus edulis). The map population consisted of 40 megagametophytes derived from one tree at Sunset Crater, Arizona. A total of 78 primer
combinations, each with three to five selective nucleotides, amplified 542 polymorphic markers. Of these, 33 markers showed
significant deviation from the expected Mendelian genotypic segregation ratio of 1 : 1, and 164 showed complete linkage with
another marker. This resulted in 338 unique markers mapping to 25 linkage groups, each of which ranged from 2 to 22 markers,
averaging 80 centiMorgans (cM) in size and covering 2,012 cM (2,200 cM with the inclusion of 25 cM for each of 7 unlinked
markers). Pairwise linkage values gave a genome size estimate of 2,390 cM, suggesting comprehensive coverage of the genome.
A search for subsets of primer combinations giving the best map coverage found 10 primer combinations which together marked
72% of the linkage map to within 10 cM; an additional 10 primer combinations increased this percentage to 85%. Our map represents
an initial step towards the identification of quantitative trait loci associated with pest resistance and water stress in
pinyons and will further allow us to examine introgression rates between P. edulis and P. californiarum.
Received: 14 October 1997 / Accepted: 29 April 1998 相似文献
11.
Genetic and physical mapping of new EST-derived SSRs on the A and B genome chromosomes of wheat 总被引:2,自引:0,他引:2
A. Gadaleta A. Giancaspro S. L. Giove S. Zacheo G. Mangini R. Simeone A. Signorile A. Blanco 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(5):1015-1025
The availability of genetic maps and phenotypic data of segregating populations allows to localize and map agronomically important
genes, and to identify closely associated molecular markers to be used in marker-assisted selection and positional cloning.
The objective of the present work was to develop a durum wheat intervarietal genetic and physical map based on genomic microsatellite
or genomic simple sequence repeats (gSSR) markers and expressed sequence tag (EST)-derived microsatellite (EST-SSR) markers.
A set of 122 new EST-SSR loci amplified by 100 primer pairs was genetically mapped on the wheat A and B genome chromosomes.
The whole map also comprises 149 gSSR markers amplified by 120 primer pairs used as anchor chromosome loci, two morphological
markers (Black colour, Bla1, and spike glaucousness, Ws) and two seed storage protein loci (Gli-A2 and Gli-B2). The majority of SSR markers tested (182) was chromosome-specific. Out of 275 loci 241 loci assembled in 25 linkage groups
assigned to the chromosomes of the A and B genome and 34 remained unlinked. A higher percentage of markers (54.4%), localized
on the B genome chromosomes, in comparison to 45.6% distributed on the A genome. The whole map covered 1,605 cM. The B genome
accounted for 852.2 cM of genetic distance; the A genome basic map spanned 753.1 cM with a minimum length of 46.6 cM for chromosome
5A and a maximum of 156.2 cM for chromosome 3A and an average value of 114.5 cM. The primer sets that amplified two or more
loci mapped to homoeologous as well as to non-homoeologous sites. Out of 241 genetically mapped loci 213 (88.4%) were physically
mapped by using the nulli-tetrasomic, ditelosomic and a stock of 58 deletion lines dividing the A and B genome chromosomes
in 94 bins. No discrepancies concerning marker order were observed but the cytogenetic maps revealed in some cases small genetic
distance covered large physical regions. Putative function for mapped SSRs were assigned by searching against GenBank nonredundant
database using TBLASTX algorithms. 相似文献
12.
Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome
下载免费PDF全文
![点击此处可从《Plant biotechnology journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Shenghui Zhou Jinpeng Zhang Yonghe Che Weihua Liu Yuqing Lu Xinming Yang Xiuquan Li Jizeng Jia Xu Liu Lihui Li 《Plant biotechnology journal》2018,16(3):818-827
Agropyron Gaertn. (P genome) is a wild relative of wheat that harbours many genetic variations that could be used to increase the genetic diversity of wheat. To agronomically transfer important genes from the P genome to a wheat chromosome by induced homoeologous pairing and recombination, it is necessary to determine the chromosomal relationships between Agropyron and wheat. Here, we report using the wheat 660K single nucleotide polymorphism (SNP) array to genotype a segregating Agropyron F1 population derived from an interspecific cross between two cross‐pollinated diploid collections ‘Z1842’ [A. cristatum (L.) Beauv.] (male parent) and ‘Z2098’ [A. mongolicum Keng] (female parent) and 35 wheat–A. cristatum addition/substitution lines. Genetic linkage maps were constructed using 913 SNP markers distributed among seven linkage groups spanning 839.7 cM. The average distance between adjacent markers was 1.8 cM. The maps identified the homoeologous relationship between the P genome and wheat and revealed that the P and wheat genomes are collinear and relatively conserved. In addition, obvious rearrangements and introgression spread were observed throughout the P genome compared with the wheat genome. Combined with genotyping data, the complete set of wheat–A. cristatum addition/substitution lines was characterized according to their homoeologous relationships. In this study, the homoeologous relationship between the P genome and wheat was identified using genetic linkage maps, and the detection mean for wheat–A. cristatum introgressions might significantly accelerate the introgression of genetic variation from Agropyron into wheat for exploitation in wheat improvement programmes. 相似文献
13.
MacRitchie D Sun G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(4):720-724
The potential of barley and wheat microsatellite markers for genetic analysis of Elymus trachycaulus complex species was evaluated. A set of 25 barley and 3 wheat microsatellite markers were tested for their ability to cross-amplify DNA from four accessions of E. trachycaulus and two accessions Pseudoroegneria spicata. Thirteen barley (52%) and two (68%) wheat primer pairs successfully amplified consistent products from both E. trachycaulus and P. spicata species. Four of the 15 successful primer pairs produced visible polymorphisms among the accessions tested. A higher successful rate of cross-species amplification of barley and wheat microsatellite markers in E. trachycaulus and P. spicata was found in this study. These primer pairs are now available for use as markers in genetic analysis of E. trachycaulus complex species. Our results suggest that publicly available wheat and barley microsatellite markers are a valuable resource for the genetic characterization of wild Triticeae species. 相似文献
14.
R. Guadagnuolo D. Savova-Bianchi J. Keller-Senften F. Felber 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(2-3):191-196
Seeds of English and Austrian populations of bearded wheatgrass (Elymus caninus L.) and sea barley (Hordeum marinum Huds.) growing in the vicinity of wheat (Triticum aestivum L.) fields were collected in order to search for evidence of the introgression of wheat traits into these wild relatives.
Seeds were sown and plants grown for subsequent analyses using morphological and genetic (isozymes, RAPD and wheat microsatellites)
markers. No F1 hybrids were found within the individuals of the two species grown, neither with morphological nor with genetic markers.
Also, no evidence of introgression of wheat traits into E. caninus was observed. However, in one individual of H. marinum which had the typical morphology of this species, numerous species-specific DNA markers of wheat were amplified, thereby
demonstrating previous hybridization. Consequently, the hybridization between wheat and H. marinum under natural conditions and the introgression of wheat traits into this wild relative seems to be possible. Our results
contribute to the risk assessment of transgenic wheat cultivation.
Received: 20 September 2000 / Accepted: 17 December 2000 相似文献
15.
R. Kölliker E. S. Jones M. C. Drayton M. P. Dupal J. W. Forster 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(2-3):416-424
Highly informative molecular markers, such as simple sequence repeats (SSRs), can greatly accelerate breeding programs. The
aim of this study was to develop and characterise a comprehensive set of SSR markers for white clover (Trifolium repens L.), which can be used to tag genes and quantitative trait loci controlling traits of agronomic interest. Sequence analysis
of 1123 clones from genomic libraries enriched for (CA)
n
repeats yielded 793 clones containing SSR loci. The majority of SSRs consisted of perfect dinucleotide repeats, only 7% being
trinucleotide repeats. After exclusion of redundant sequences and SSR loci with less than 25 bp of flanking sequence, 397
potentially useful SSRs remained. Primer pairs were designed for 117 SSR loci and PCR products in the expected size range
were amplified from 101 loci. These markers were highly polymorphic, 88% detecting polymorphism across seven white clover
genotypes with an average allele number of 4.8. Four primer pairs were tested in an F2 population revealing Mendelian segregation. Successful cross-species amplification was achieved in at least one out of eight
legume species for 46 of 54 primer pairs. The rate of successful amplification was significantly higher for Trifolium species when compared to species of other genera. The markers developed in this study not only provide valuable tools for
molecular breeding of white clover but may also have applications in related taxa.
Received: 3 April 2000 / Accepted: 12 May 2000 相似文献
16.
Development of AFLP markers in barley 总被引:36,自引:0,他引:36
To investigate the application of amplified fragment length polymorphism (AFLP) markers in barley, 96 primer combinations
were used to generate AFLP patterns with two barley lines, L94 and Vada. With seven primer combinations, only a few intense
bands were obtained, probably derived from repeated sequences. With the majority of the remaining 89 primer combinations,
on average about 120 amplification products were generated, and the polymorphism rate between the two lines was generally
over 18%. Based on the number of amplified products and the polymorphism rate, the 48 best primer combinations were selected
and tested on 16 barley lines, again including L94 and Vada. Using a subset of 24 primer combinations 2188 clearly visible
bands within the range from 80 to 510 bp were generated; 55% of these showed some degree of polymorphism among the 16 lines.
L94 versus Vada showed the highest polymorphism rate (29%) and Proctor versus Nudinka yielded the lowest (12%). The polymorphism
rates per primer combination showed little dependence on the barley lines used. Hence the most efficient and informative primer
combinations identified for a given pair of lines turned out to be highly efficient when applied to others. Generally, more
than 100 common markers (possibly locus specific) among populations or crosses were easily identified by comparing 48 AFLP
profiles of the parent lines. The existence of such a large number of markers common to populations will facilitate the merging
of molecular marker data and other genetic data into one integrated genetic map of barley.
Received: 28 October 1996 / Accepted: 27 November 1996 相似文献
17.
B. Saal J. Plieske J. Hu C. F. Quiros D. Struss 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(5):695-699
Microsatellites are highly polymorphic and efficient markers for the analysis of plant genomes. Primer specificity, however,
may restrict the applicability of these markers even between closely related species for comparative mapping studies. We have
demonstrated that the majority of microsatellites identified in oilseed rape (Brassica napus L; AC genome) correspond to loci which can be easily assigned to the A and C progenitor genomes. A study with 63 primer pairs
has shown that 54% detect two loci, one from each genome, while 25% and 21%, respectively, are either A or C genome-specific.
The distribution of rapeseed microsatellites in the C genome was investigated by genetic mapping in Brassica oleracea L. Ninety two dinucleotide microsatellites were screened for polymorphism in an F2 population derived from a cross between collard and cauliflower, for which an RFLP map has been constructed previously. Thirty
three primer pairs (35.7%) have yielded either unspecific or no PCR products whereas the remaining primer pairs amplified
one or more distinct loci. The level of polymorphism found in the mapping population was 49.2%. A total of 29 primer pairs
disclosed 34 loci of which 31 are evenly distributed on 8 of the 9 B. oleracea linkage groups. For the remaining three markers linkage could not be established. Our results showed that microsatellite
markers from the composite genome of B. napus can serve as a useful marker system in genetic studies and for plant-breeding objectives in B. oleracea.
Received: 14 April 2000 / Accepted: 3 July 2000 相似文献
18.
Li-Jun Hu Cheng Liu Zi-Xian Zeng Guang-Rong Li Xiao-Jin Song Zu-Jun Yang 《Genes & genomics.》2012,34(1):67-75
Thinopyrum elongatum serves as an excellent gene pool for wheat improvement. Genes for resistance to many biotic and abiotic stresses have been transferred from Th. elongatum to wheat through chromosome manipulation. For breeding programs, molecular markers enable screening of a large number of genotypes for alien chromosome introgressions. The main objective of the present study was to develop and characterize EST (expressed sequence tags) and PLUG (PCR-based Landmark Unique Gene) markers that can distinguish Th. elongatum chromatin from the wheat genomes. A total of 258 mapped EST primer pairs and 46 PLUG primer pairs were tested on DNA from wheat Chinese Spring (CS) and CS-Th. elongatum addition lines. The results showed that 43 primer pairs could be effectively mapped to specific Th. elongatum chromosomes. Twenty-two of the 43 markers displayed similar homoeologous chromosome locations to hexaploid wheat. Nine markers mapped to different linkage groups between wheat and Th. elongatum, while 12 makers mapped on two or three different Th. elongatum chromosomes. A comparison of molecular marker locations indicated that Th. elongatum genome was closely related to the D genome of wheat, and chromosome rearrangements and duplication had occurred in Th. elongatum and the wheat genomes. The markers will be useful in comparative gene mapping, chromosome evolutionary analysis, and gene introgression for wheat improvement using Th. elongatum accessions as gene donors. 相似文献
19.
Slavov GT Howe GT Yakovlev I Edwards KJ Krutovskii KV Tuskan GA Carlson JE Strauss SH Adams WT 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(5):873-880
Twenty-two highly variable SSR markers were developed in Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of all 22 SSRs was confirmed via segregation analyses in several Douglas-fir families. The mean observed heterozygosity and the mean number of alleles per locus were 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Twenty markers were used in genetic linkage analysis and mapped to ten known linkage groups. Because of their high polymorphism and unambiguous phenotypes, 15 single-locus markers were selected as the most suitable for DNA fingerprinting and parentage analysis. Only three SSRs were sufficient to achieve an average probability of exclusion from paternity of 0.998 in a Douglas-fir seed orchard block consisting of 59 parents.Communicated by O. Savolainen 相似文献
20.
M. M. Nachit I. Elouafi A. Pagnotta A. El Saleh E. Iacono M. Labhilili A. Asbati M. Azrak H. Hazzam D. Benscher M. Khairallah J.-M. Ribaut O. A. Tanzarella E. Porceddu M. E. Sorrells 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(2-3):177-186
Durum wheat (Triticum turgidum L. var. durum) is an economically and nutritionally important cereal crop in the Mediterranean region. To further our understanding
of durum genome organization we constructed a durum linkage map using restriction fragment length polymorphisms (RFLPs), simple
sequence repeats (SSRs) known as Gatersleben wheat microsatellites (GWMs), amplified fragment length polymorphisms (AFLPs),
and seed storage proteins (SSPs: gliadins and glutenins). A population of 110 F9 recombinant inbred lines (RILs) was derived from an intraspecific cross between two durum cultivars, Jennah Khetifa and Cham
1. The two parents exhibit contrasting traits for resistance to biotic and abiotic stresses and for grain quality. In total,
306 markers have been placed on the linkage map – 138 RFLPs, 26 SSRs, 134 AFLPs, five SSPs, and three known genes (one pyruvate
decarboxylase and two lipoxygenases). The map is 3598 cM long, with an average distance between markers of 11.8 cM, and 12.1%
of the markers deviated significantly from the expected Mendelian ratio 1:1. The molecular markers were evenly distributed
between the A and B genomes. The chromosome with the most markers is 1B (41 markers), followed by 3B and 7B, with 25 markers
each. The chromosomes with the fewest markers are 2A (11 markers), 5A (12 markers), and 4B (15 markers). In general, there
is a good agreement between the map obtained and the Triticeae linkage consensus maps. This intraspecific map provides a useful tool for marker-assisted selection and map-based breeding
for resistance to biotic and abiotic stresses and for improvement of grain quality.
Received: 14 February 2000 / Accepted: 28 April 2000 相似文献