共查询到20条相似文献,搜索用时 31 毫秒
1.
Kanervo E Singh M Suorsa M Paakkarinen V Aro E Battchikova N Aro EM 《Plant & cell physiology》2008,49(3):396-410
The protein complexes of pea (Pisum sativum L.) etioplasts,etio-chloroplasts and chloroplasts were examined using 2D BlueNative/SDS–PAGE. The most prominent protein complexesin etioplasts were the ATPase and the Clp and FtsH proteasecomplexes which probably have a crucial role in the biogenesisof etioplasts and chloroplasts. Also the cytochrome b6f (Cytb6f) complex was assembled in the etioplast membrane, as wellas Rubisco, at least partially, in the stroma. These complexesare composed of proteins encoded by both the plastid and nucleargenomes, indicating that a functional cross-talk exists betweenpea etioplasts and the nucleus. In contrast, the proteins andprotein complexes that bind chlorophyll, with the PetD subunitand the entire Cyt b6f complex as an exception, did not accumulatein etioplasts. Nevertheless, some PSII core components suchas PsbE and the luminal oxygen-evolvong complex (OEC) proteinsPsbO and PsbP accumulated efficiently in etioplasts. After 6h de-etiolation, a complete PSII core complex appeared with40% of the maximal photochemical efficiency, but a fully functionalPSII was recorded only after 24 h illumination. Similarly, thecore complex of PSI was assembled after 6 h illumination, whereasthe PSI–light-harvesting complex I was stably assembledonly in chloroplasts illuminated for 24 h. Moreover, a batteryof proteins responsible for defense against oxidative stressaccumulated particularly in etioplasts, including the stromaland thylakoidal forms of ascorbate peroxidase, glutathione reductaseand PsbS. 相似文献
2.
PsbZ (Ycf9) is a membrane protein of PSII complexes and is highly conserved from cyanobacteria to plants. We deleted the psbZ gene in the thermophilic cyanobacterium, Thermosynechococcus elongatus. The mutant cells showed photoautotrophic growth indistinguishable from that of the wild type under low and standard light conditions, while they showed even better growth than the wild type under high light. The mutant accumulated less carotenoids and more phycobiliproteins than the wild type under high light, suggestive of tolerance to photoinhibition. The mutant cells evolved oxygen at a rate comparable with the wild type, while the PSII complex isolated from the mutant retained much lower activity than the wild type. N-terminal sequencing revealed that Ycf12 and PsbK proteins were almost lost in the PSII complex. These results indicate that PsbZ is involved in functional integrity of the PSII complex by stabilizing PsbK and Ycf12. We suggest that Ycf12 is an unidentified membrane-spanning polypeptide that is placed near PsbZ and PsbK in the crystal structure of PSII. 相似文献
3.
Ishida Satoshi; Takabayashi Atsushi; Ishikawa Noriko; Hano Yasushi; Endo Tsuyoshi; Sato Fumihiko 《Plant & cell physiology》2009,50(2):383-393
The chloroplast NAD(P)H dehydrogenase (NDH) complex, which reducesplastoquinones in thylakoid membranes, is involved in PSI cyclicelectron flow and chlororespiration. In addition to land plants,the NDH complex is conserved in cyanobacteria. In this study,we identified a novel NDH-related gene of Arabidopsis, NDH-dependentcyclic electron flow 5 (NDF5, At1g55370). Post-illuminationincreases in chlorophyll fluorescence were absent in ndf5 mutantplants, which indicated that NDF5 is essential for NDH activity.Sequence analysis did not reveal any known functional motifsin NDF5, but there was some homology in amino acid sequencebetween NDF5 and NDF2, a known NDH subunit. NDF5 and NDF2 homologswere present in higher plants, but not cyanobacteria. A singlehomolog, which had similarity to both NDF5 and NDF2, was identifiedin the moss Physcomitrella patens. Immunoblot analysis showedthat NDF5 localizes to membrane fractions of chloroplasts. Thestability of NdhH, a subunit of the NDH complex, as well asNDF5 and NDF2, was decreased in ndf5, ndf2 and double ndf2/ndf5mutants, resulting in a loss of NDH activity in these mutants.These results indicated that both NDF5 and NDF2 have essentialfunctions in the stabilization of the NDH complex. We proposethat NDF5 and NDF2 were acquired by land plants during evolution,and that in higher plants both NDF5 and NDF2 are critical toregulate NDH activity and each other's protein stability, aswell as the stability of additional NDH subunits. 相似文献
4.
Identification of dynamin as an interactor of rice GIGANTEA by tandem affinity purification (TAP) 总被引:1,自引:0,他引:1
GIGANTEA (GI), CONSTANS (CO) and FLOWERING LOCUS T (FT) regulatephotoperiodic flowering in Arabidopsis. In rice, OsGI, Hd1 andHd3a were identified as orthologs of GI, CO and FT, respectively,and are also important regulators of flowering. Although GIhas roles in both flowering and the circadian clock, our understandingof its biochemical functions is still limited. In this study,we purified novel OsGI-interacting proteins by using the tandemaffinity purification (TAP) method. The TAP method has beenused effectively in a number of model species to isolate proteinsthat interact with proteins of interest. However, in plants,the TAP method has been used in only a few studies, and no novelproteins have previously been isolated by this method. We generatedtransgenic rice plants and cell cultures expressing a TAP-taggedversion of OsGI. After a two-step purification procedure, theinteracting proteins were analyzed by mass spectrometry. Sevenproteins, including dynamin, were identified as OsGI-interactingproteins. The interaction of OsGI with dynamin was verifiedby co-immunoprecipitation using a myc-tagged version of OsGI.Moreover, an analysis of Arabidopsis dynamin mutants indicatedthat although the flowering times of the mutants were not differentfrom those of wild-type plants, an aerial rosette phenotypewas observed in the mutants. We also found that OsGI is presentin both the nucleus and the cytosol by Western blot analysisand by transient assays. These results indicate that the TAPmethod is effective for the isolation of novel proteins thatinteract with target proteins in plants. 相似文献
5.
6.
Nitric oxide (NO) is a gas displaying multiple physiologicalfunctions in plants, animals and bacteria. The enzymes nitratereductase and NO synthase have been suggested to be involvedin the production of NO in plants and algae, but the implicationof those enzymes in NO production under physiological conditionsremains obscure. Symbiodinium microadriaticum, commonly referredto as zooxanthellae, is a marine microalga commonly found insymbiotic association with a cnidarian host including reef-buildingcorals. Here we demonstrate NO production in zooxanthellae uponsupplementation of either sodium nitrite or L-arginine as asubstrate. The nitrite-dependent NO production was detectedelectrochemically and confirmed by the application of 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide(cPTIO), a specific NO scavenger. Cells stained with the diaminofluorescein,DAF-2 DA, an NO fluorescent probe, showed an increase in fluorescenceintensity upon supplementation of both sodium nitrite and L-arginine.Microscopic observations of DAF-stained cells verified thatNO was produced inside the cells. NO production in S. microadriaticumwas found to increase upon exposure of cells to an acute heatstress which also caused a decline in the photosynthetic efficiencyof PSII (Fv/Fm). This study provides substantial evidence toconfirm that zooxanthellae can synthesize NO even when theyare not in a symbiotic association with a coral host. The increasein NO production at high temperatures suggests that heat stressstimulates the microalgal NO production in a temperature-dependentmanner. The implications of these findings are discussed inthe light of the coral bleaching phenomenon which is associatedwith elevated sea surface temperature due to global warming. 相似文献
7.
Shi Lan-Xin Kim Soo J. Marchant Alan Robinson Colin Schröder Wolfgang P. 《Plant molecular biology》1999,40(4):737-744
The location and expression of the previously uncharacterised photosystem II subunit PsbX have been analysed in higher plants. We show that this protein is a component of photosystem II (PSII) core particles but absent from light-harvesting complexes or PSII reaction centres. PsbX is, however, localised to the near vicinity of the reaction centre because it can be cross-linked to cytochrome b559, which is known to be associated with the D1/D2 dimer. We also show that the expression of this protein is tightly regulated by light, since neither protein nor mRNA is found in dark-grown plants. 相似文献
8.
A desiccation-tolerant cyanobacterium, Nostoc commune, showsunique responses to dehydration. These responses are: (i) lossof PSII activity in parallel with the loss of photosynthesis;(ii) loss of PSI activity; and (iii) dissipation of light energyabsorbed by pigment–protein complexes. In this study,the deactivation of PSII is shown to be important in avoidingphotoinhibition when the Calvin–Benson cycle is repressedby dehydration. Furthermore, our evidence suggests that dissipationof light energy absorbed by PSII blocks photoinhibition understrong light in dehydrated states. 相似文献
9.
Shi LX Lorković ZJ Oelmuller R Schroder WP 《The Journal of biological chemistry》2000,275(48):37945-37950
Arabidopsis thaliana plants have been transformed with an antisense gene to the psbW of photosystem II (PSII). Eight transgenic lines containing low levels of psbW mRNA have been obtained. Transgenic seedlings with low contents of PsbW protein (more than 96% reduced) were selected by Western blotting and used for photosynthetic functional studies. There were no distinct differences in phenotype between the antisense and wild type plants during vegetative period under normal growth light intensities. However, a sucrose gradient separation of briefly solubilized thylakoid membranes revealed that no dimeric PSII supracomplex could be detected in the transgenic plants lacking the PsbW protein. Furthermore, analysis of isolated thylakoids demonstrated that the oxygen-evolving rate in antisense plants decreased by 50% compared with the wild type. This was found to be due to up to 40% of D1 and D2 reaction center proteins of PSII disappearing in the transgenic plants. The absence of the PsbW protein also altered the contents of other PSII proteins to differing extents. These results show that in the absence of the PsbW protein, the stability of the dimeric PSII is diminished and consequently the total number of PSII complexes is greatly reduced. Thus the nuclear encoded PsbW protein may play a crucial role in the biogenesis and regulation of the photosynthetic apparatus. 相似文献
10.
Schwenkert S Umate P Dal Bosco C Volz S Mlçochová L Zoryan M Eichacker LA Ohad I Herrmann RG Meurer J 《The Journal of biological chemistry》2006,281(45):34227-34238
Photosystem II (PSII) core complexes consist of CP47, CP43, D1, D2 proteins and of several low molecular weight integral membrane polypeptides, such as the chloroplast-encoded PsbE, PsbF, and PsbI proteins. To elucidate the function of PsbI in the photosynthetic process as well as in the biogenesis of PSII in higher plants, we generated homoplastomic knock-out plants by replacing most of the tobacco psbI gene with a spectinomycin resistance cartridge. Mutant plants are photoautotrophically viable under green house conditions but sensitive to high light irradiation. Antenna proteins of PSII accumulate to normal amounts, but levels of the PSII core complex are reduced by 50%. Bioenergetic and fluorescence studies uncovered that PsbI is required for the stability but not for the assembly of dimeric PSII and supercomplexes consisting of PSII and the outer antenna (PSII-LHCII). Thermoluminescence emission bands indicate that the presence of PsbI is required for assembly of a fully functional Q(A) binding site. We show that phosphorylation of the reaction center proteins D1 and D2 is light and redox-regulated in the wild type, but phosphorylation is abolished in the mutant, presumably due to structural alterations of PSII when PsbI is deficient. Unlike wild type, phosphorylation of LHCII is strongly increased in the dark due to accumulation of reduced plastoquinone, whereas even upon state II light phosphorylation is decreased in delta psbI. These data attest that phosphorylation of D1/D2, CP43, and LHCII is regulated differently. 相似文献
11.
The chilling injury induced by high root temperature in the leaves of rice seedlings 总被引:2,自引:0,他引:2
Root temperature is found to be a very important factor forleaves to alter the response and susceptibility to chillingstress. Severe visible damage was observed in the most activeleaves of seedlings of a japonica rice (Oryza sativa cv. Akitakomachi),e.g. the third leaf at the third-leaf stage, after the treatmentwhere only leaves but not roots were chilled (L/H). On the otherhand, no visible damage was observed after the treatment whereboth leaves and roots were chilled simultaneously (L/L). Thechilling injury induced by L/H, a novel type of chilling injury,required the light either during or after the chilling in orderto develop the visible symptoms such as leaf bleaching and tissuenecrosis. Chlorophyll fluorescence parameters measured aftervarious lengths of chilling treatments showed that significantchanges were induced before the visible injury. The effectivequantum yield and photochemical quenching of PSII dropped dramaticallywithin 24 h in both the presence and absence of a 12 h lightperiod. The maximal quantum yield and non-photochemical quenchingof PSII decreased significantly only in the presence of light.On the other hand, L/H chilling did not affect the functionof PSI, but caused a significant decrease in the electron availabilityfor PSI. These results suggest that the leaf chilling with highroot temperature destroys some component between PSII and PSIwithout the aid of light, which causes the over-reduction ofPSII in the light, and thereby the visible injury is inducedonly in the light. 相似文献
12.
Novel Cysteine-Rich Peptides from Digitaria ciliaris and Oryza sativa Enhance Tolerance to Cadmium by Limiting its Cellular Accumulation 总被引:1,自引:0,他引:1
Kuramata Masato; Masuya Shuichi; Takahashi Yoshihiro; Kitagawa Etsuko; Inoue Chihiro; Ishikawa Satoru; Youssefian Shohab; Kusano Tomonobu 《Plant & cell physiology》2009,50(1):106-117
By means of functional screening using the cadmium (Cd)-sensitiveycf1 yeast mutant, we have isolated a novel cDNA clone, DcCDT1,from Digitaria ciliaris growing in a former mining area in northernJapan, and have shown that it confers Cd tolerance to the yeastcells, which accumulated almost 2-fold lower Cd levels thancontrol cells. The 521 bp DcCDT1 cDNA contains an open readingframe of 168 bp and encodes a deduced peptide, DcCDT1, thatis 55 amino acid residues in length, of which 15 (27.3%) arecysteine residues. Five DcCDT1 homologs (here termed OsCDT1–OsCDT5)have been identified in rice, and all of them were up-regulatedto varying degrees in the above-ground tissues by CdCl2 treatment.Localization of green fluorescent protein fusions suggests thatDcCDT1 and OsCDT1 are targeted to both cytoplasmic membranesand cell walls of plant cells. Transgenic Arabidopsis thalianaplants overexpressing DcCDT1 or OsCDT1 displayed a Cd-tolerantphenotype and, consistent with our yeast data, accumulated loweramounts of Cd when grown on CdCl2. Collectively, our data suggestthat DcCDT1 and OsCDT1 function to prevent entry of Cd intoyeast and plant cells and thereby enhance their Cd tolerance. 相似文献
13.
14.
15.
The AtXTH28 Gene, a Xyloglucan Endotransglucosylase/Hydrolase, is Involved in Automatic Self-Pollination in Arabidopsis thaliana 总被引:1,自引:0,他引:1
Kurasawa Kasumi; Matsui Akihiro; Yokoyama Ryusuke; Kuriyama Tomoko; Yoshizumi Takeshi; Matsui Minami; Suwabe Keita; Watanabe Masao; Nishitani Kazuhiko 《Plant & cell physiology》2009,50(2):413-422
Successful automatic self-pollination in flowering plants isdependent on the correct development of reproductive organs.In the stamen, the appropriate growth of the filament, whichlargely depends on the mechanical properties of the cell wall,is required to position the anther correctly close to the stigmaat the pollination stage. Xyloglucan endotransglucosylase/hydrolases(XTHs) are a family of enzymes that mediate the constructionand restructuring of xyloglucan cross-links, thereby controllingthe extensibility or mechanical properties of the cell wallin a wide variety of plant tissues. Our reverse genetic analysishas revealed that a loss-of-function mutation of an ArabidopsisXTH family gene, AtXTH28, led to a decrease in capability forself-pollination, probably due to inhibition of stamen filamentgrowth. Our results also suggest that the role of AtXTH28 inthe development of the stamen is not functionally redundantwith its closest paralog, AtXTH27. Thus, our finding indicatesthat AtXTH28 is specifically involved in the growth of stamenfilaments, and is required for successful automatic self-pollinationin certain flowers in Arabidopsis thaliana. 相似文献
16.
Armbruster U Zühlke J Rengstl B Kreller R Makarenko E Rühle T Schünemann D Jahns P Weisshaar B Nickelsen J Leister D 《The Plant cell》2010,22(10):3439-3460
Photosystem II (PSII) is a multiprotein complex that functions as a light-driven water:plastoquinone oxidoreductase in photosynthesis. Assembly of PSII proceeds through a number of distinct intermediate states and requires auxiliary proteins. The photosynthesis affected mutant 68 (pam68) of Arabidopsis thaliana displays drastically altered chlorophyll fluorescence and abnormally low levels of the PSII core subunits D1, D2, CP43, and CP47. We show that these phenotypes result from a specific decrease in the stability and maturation of D1. This is associated with a marked increase in the synthesis of RC (the PSII reaction center-like assembly complex) at the expense of PSII dimers and supercomplexes. PAM68 is a conserved integral membrane protein found in cyanobacterial and eukaryotic thylakoids and interacts in split-ubiquitin assays with several PSII core proteins and known PSII assembly factors. Biochemical analyses of thylakoids from Arabidopsis and Synechocystis sp PCC 6803 suggest that, during PSII assembly, PAM68 proteins associate with an early intermediate complex that might contain D1 and the assembly factor LPA1. Inactivation of cyanobacterial PAM68 destabilizes RC but does not affect larger PSII assembly complexes. Our data imply that PAM68 proteins promote early steps in PSII biogenesis in cyanobacteria and plants, but their inactivation is differently compensated for in the two classes of organisms. 相似文献
17.
Frelet-Barrand A Kolukisaoglu HU Plaza S Rüffer M Azevedo L Hörtensteiner S Marinova K Weder B Schulz B Klein M 《Plant & cell physiology》2008,49(4):557-569
The enormous metabolic plasticity of plants allows detoxificationof many harmful compounds that are generated during biosyntheticprocesses or are present as biotic or abiotic toxins in theirenvironment. Derivatives of toxic compounds such as glutathioneconjugates are moved into the central vacuole via ATP-bindingcassette (ABC)-type transporters of the multidrug resistance-associatedprotein (MRP) subfamily. The Arabidopsis genome contains 15AtMRP isogenes, four of which (AtMRP1, 2, 11 and 12) clustertogether in one of two major phylogenetic clades. We isolatedT-DNA knockout alleles in all four highly homologous AtMRP genesof this clade and subjected them to physiological analysis toassess the function of each AtMRP of this group. None of thesingle atmrp mutants displayed visible phenotypes under controlconditions. In spite of the fact that AtMRP1 and AtMRP2 hadbeen described as efficient ATP-dependent organic anion transportersin heterologous expression experiments, the contribution ofthree of the AtMRP genes (1, 11 and 12) to detoxification ismarginal. Only knockouts in AtMRP2 exhibited a reduced sensitivitytowards 1-chloro-2,4-dinitrobenzene, but not towards other herbicides.AtMRP2 but not AtMRP1, 11 and 12 is involved in chlorophylldegradation since ethylene-treated rosettes of atmrp2 showedreduced senescence, and AtMRP2 expression is induced duringsenescence. This suggests that AtMRP2 is involved in vacuolartransport of chlorophyll catabolites. Vacuolar uptake studiesdemonstrated that transport of typical MRP substrates was reducedin atmrp2. We conclude that within clade I, only AtMRP2 contributessignificantly to overall organic anion pump activity in vivo. 相似文献
18.
Fujiwara MT Hashimoto H Kazama Y Abe T Yoshida S Sato N Itoh RD 《Plant & cell physiology》2008,49(3):345-361
Chloroplast division comprises a sequence of events that facilitatesymmetric binary fission and that involve prokaryotic-like stromaldivision factors such as tubulin-like GTPase FtsZ and the divisionsite regulator MinD. In Arabidopsis, a nuclear-encoded prokaryoticMinE homolog, AtMinE1, has been characterized in terms of itseffects on a dividing or terminal chloroplast state in a limitedseries of leaf tissues. However, the relationship between AtMinE1expression and chloroplast phenotype remains to be fully elucidated.Here, we demonstrate that a T-DNA insertion mutation in AtMinE1results in a severe inhibition of chloroplast division, producingmotile dots and short filaments of FtsZ. In AtMinE1 sense (overexpressor)plants, dividing chloroplasts possess either single or multipleFtsZ rings located at random intervals and showing constrictiondepth, mainly along the chloroplast polarity axis. The AtMinE1sense plants displayed equivalent chloroplast phenotypes toarc11, a loss-of-function mutant of AtMinD1 which forms replicatingmini-chloroplasts. Furthermore, a certain population of FtsZrings formed within developing chloroplasts failed to initiateor progress the membrane constriction of chloroplasts and consequentiallyto complete chloroplast fission in both AtMinE1 sense and arc11/atminD1plants. Our present data thus demonstrate that the chloroplastdivision site placement involves a balance between the opposingactivities of AtMinE1 and AtMinD1, which acts to prevent FtsZring formation anywhere outside of the mid-chloroplast. In addition,the imbalance caused by an AtMinE1 dominance causes multiple,non-synchronous division events at the single chloroplast level,as well as division arrest, which becomes apparent as the chloroplastsmature, in spite of the presence of FtsZ rings. 相似文献
19.
Matsumoto Tadashi; Lian Hong-Li; Su Wei-Ai; Tanaka Daisuke; Liu Cheng wei; Iwasaki Ikuko; Kitagawa Yoshichika 《Plant & cell physiology》2009,50(2):216-229
Although an association between chilling tolerance and aquaporinshas been reported, the exact mechanisms involved in this relationshipremain unclear. We compared the expression profiles of aquaporingenes between a chilling-tolerant and a low temperature-sensitiverice variety using real-time PCR and identified seven genesthat closely correlated with chilling tolerance. Chemical treatmentexperiments, by which rice plants were induced to lose theirchilling tolerance, implicated the PIP1 (plasma membrane intrinsicprotein 1) subfamily member genes in chilling tolerance. Ofthese members, changes in expression of the OsPIP1;3 gene suggestedthis to be the most closely related to chilling tolerance. AlthoughOsPIP1;3 showed a much lower water permeability than membersof the OsPIP2 family, OsPIP1;3 enhanced the water permeabilityof OsPIP2;2 and OsPIP2;4 when co-expressed with either of theseproteins in oocytes. Transgenic rice plants (OE1) overexpressingOsPIP1;3 showed an enhanced level of chilling tolerance andthe ability to maintain high OsPIP1;3 expression levels underlow temperature treatment, similar to that of chilling-tolerantrice plants. We assume that OsPIP1;3, constitutively overexpressedin the leaf and root of transgenic OE1 plants, interacts withmembers of the OsPIP2 subfamily, thereby improving the plantswater balance under low temperatures and resulting in the observedchilling tolerance of the plants. 相似文献
20.
Involvement of Reactive Nitrogen and Oxygen Species (RNS and ROS) in Sunflower-Mildew Interaction 总被引:1,自引:0,他引:1
Chaki Mounira; Fernandez-Ocana Ana M.; Valderrama Raquel; Carreras Alfonso; Esteban Francisco J.; Luque Francisco; Gomez-Rodriguez Maria V.; Begara-Morales Juan C.; Corpas Francisco J.; Barroso Juan B. 《Plant & cell physiology》2009,50(2):265-279
Nitric oxide (·NO) has been shown to participate in plantresponse against pathogen infection; however, less is knownof the participation of other NO-derived molecules designatedas reactive nitrogen species (RNS). Using two sunflower (Helianthusannuus L.) cultivars with different sensitivity to infectionby the pathogen Plasmopara halstedii, we studied key componentsinvolved in RNS and ROS metabolism. We analyzed the superoxideradical production, hydrogen peroxide content, L-arginine-dependentnitric oxide synthase (NOS) and S-nitrosoglutathione reductase(GSNOR) activities. Furthermore, we examined the location andcontents of ·NO, S-nitrosothiols (RSNOs), S-nitrosoglutathione(GSNO) and protein 3-nitrotyrosine (NO2-Tyr) by confocal laserscanning microscopy (CLSM) and biochemical analyses. In thesusceptible cultivar, the pathogen induces an increase in proteinsthat undergo tyrosine nitration accompanied by an augmentationin RSNOs. This rise of RSNOs seems to be independent of theenzymatic generation of ·NO because the L-arginine-dependentNOS activity is reduced after infection. These results suggestthat pathogens induce nitrosative stress in susceptible cultivars.In contrast, in the resistant cultivar, no increase of RSNOsor tyrosine nitration of proteins was observed, implying anabsence of nitrosative stress. Therefore, it is proposed thatthe increase of tyrosine nitration of proteins can be considereda general biological marker of nitrosative stress in plantsunder biotic conditions. 相似文献