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1.
2.
The aim of this study was developing coordination complexes that can be used as inorganic medicinal agents. The water soluble [Pt(phen)(His)]NO(3)·3H(2)O complex in which phen=1,10-phenantheroline and His=L-histidine was synthesized and characterized using physicochemical methods. Binding interaction of this complex with calf thymus (CT) DNA was investigated by emission, absorption, circular dichroism, and viscosity measurement techniques. Upon addition of CT-DNA, changes were observed in the characteristic ultraviolet-visible (UV-Vis) bands (hypochromism) of the complex. The complex binds to CT-DNA in an intercalative mode. The calculated binding constant, K(b), was 8 ± 0.2 × 10(4) M(-1). In addition, circular dichroism (CD) study showed that the phenanthroline ligand was inserted between the base pair stack of the double-helical structure of DNA. Also, the fluorescence spectral characteristics showed an increase in fluorescence intensity of the platinum complex in the presence of increasing amounts of DNA solution. The experimental results showed that the platinum complex binds to DNA via intercalative and hydrogen bonding mode. 相似文献
3.
The binding of [Dy(dmp)2Cl3(OH2)], where dmp is 2,9-dimethyl 1,10-phenanthroline, with Fish salmon DNA (FS-DNA) is investigated by absorption and emission spectroscopy, quenching studies, salt dependent, and gel electrophoresis. The binding constant (Kb) of the interaction is calculated as (1.27 ± .05) × 105 M?1 from absorption spectral titration data. The Stern–Volmer constant (KSV), thermodynamic parameters involves ΔG°, ?H°, and ?S° are calculated by fluorescent data and Van’t Hoff equation. The thermodynamic studies show that the reaction for the binding of the complex with FS-DNA is endothermic and entropically driven (ΔS° > 0, ΔH° > 0). The effect of the complex concentration on FS-DNA cleavage reactions is also investigated by gel electrophoresis. Furthermore, the Dy(III) complex has been screened for its antibacterial activity. The experimental results suggest that the Dy(III) complex binds significantly to FS-DNA by hydrophobic groove binding mode and the complex has more efficient antibacterial activity compared to its metal salt. 相似文献
4.
Absorption spectroscopy and circular dichroism (CD) have been used to characterize the DNA binding of [Fe(phen)3]2+, [Fe(phen)2(DIP)]2+ and [Fe(phen)(DIP)2]2+ where phen and DIP stand for 1,10-phenanthroline and 4,7-diphenyl-1,10-phenanthroline, respectively. Both [Fe(phen)3]2+ and [Fe(phen)2(DIP)]2+ bind weakly to calf thymus DNA (CT-DNA) in an electrostatic mode, while [Fe(phen)(DIP)2]2+ binds more strongly to CT-DNA, possibly in an intercalation mode. The hypochromicity, red shift and Kb increase in the order [Fe(phen)3]2+ < [Fe(phen)2(DIP)]2+ < [Fe(phen)(DIP)2]2+ in accordance with the increase in size and hydrophobicity of the iron(II) complexes. The thermodynamic parameters obtained suggest that the DNA binding of both [Fe(phen)3]2+ and [Fe(phen)2(DIP)]2+ is entropically driven, while that of [Fe(phen)(DIP)2]2+ is enthalpically driven. A strong CD spectrum in the UV and visible region develops upon addition of CT-DNA into the racemate solution of each iron(II) complex (Pfeiffer effect). This has revealed that a shift in diastereomeric inversion equilibrium takes place in the solution to yield an excess of one of the DNA-complex diastereomers. The striking resemblance of the CD spectral profiles to those of the pure delta-enantiomer indicates that the delta-enantiomer of the iron(II) complexes is preferentially bound to CT-DNA. The mechanism of the development of Pfeiffer CD is proposed on the basis of kinetic studies on the DNA binding of the racemic iron(II) complexes. 相似文献
5.
Bruce E. Green Colin H.L. Kennard Graham Smith Margaret M. Elcombe Frank H. Moore Bruce D. James Allan H. White 《Inorganica chimica acta》1984,83(3):177-189
Copper(I) is five coordinate in (1,10-phenanthroline)tetrahydroborato(triphenylphosphine)copper(I). This compound crystallizes from either toluene as the yellow, α-form, a = 16.247(8), b = 9.750(7), c = 9.322(5) Å, α = 62.92(4), β = 84.77(4), γ = 84.34(5)°, triclinic P, Z = 2, or from a xylene/methylene chloride mixture as the red β-form, X-ray cell, a = 13.675(11), b = 10.115(8), c = 9.700(7) Å, α = 95.22(6), β = 96.22(6), γ = 101.02(6)°; neutron cell, as the tetradeuteroborate, a = 13.703(1), b = 10.096(8), c = 9.74(1) Å, α = 95.23(9), β = 96.51(8), γ = 101.04(2)°, triclinic, P, Z = 2. For both forms, unidentate triphenylphosphine, bidentate 1,10-phenanthroline and unsymmetrical bidentate BH4? completes the copper(I) coordination but there are subtle differences between the two. When the ligand 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline, dmdp, replaces 1,10-phenanthroline, the compound obtained is four coordinate with no tpp in the crystal. [C(dmdp)BH4] is monoclinic, Cc, a = 14.522(4), b = 20.07(2), c = 7.718(2) Å, β = 106.17(2)°, Z = 4. 相似文献
6.
Zohreh Moradi Ali Reza Rezvani Meissam Noroozifar 《Journal of biomolecular structure & dynamics》2019,37(9):2283-2295
In this study, fluorescence emission spectra, UV–vis absorption spectra, ethidium bromide (EB)-competition experiment, and iodide quenching experiment were used for the interaction study of the Fish salmon DNA (FS-DNA) with [Pr(dmp)2Cl3(OH2)] where dmp is 2,9-dimethyl 1,10-phenanthroline. The binding constant and the number of binding sites of the complex with FS-DNA were 6.09?±?0.04 M?1 and 1.18, respectively. The free energy, enthalpy, and entropy changes (ΔG°, ΔH°, and ΔS°) in the binding process of the Pr(III) complex with FS-DNA were –8.02?kcal mol?1, +39.44?kcal mol?1, and +159.56?cal mol?1 K?1, respectively. Based on these results, the interaction process between FS-DNA with [Pr(dmp)2Cl3(OH2)] was spontaneous and the main binding interaction force was groove binding mode. Also, Fluorescence and electronic absorption spectroscopy were used in order to evaluate the binding characteristics, stoichiometry, and interaction mode of praseodymium(III) (Pr(III)) complex with bovine serum albumin (BSA). Title complex showed good binding propensity to BSA presenting moderately high Kb values. The fluorescence quenching of BSA by Pr(III) complex has been observed to be the static process. The positive ΔH° and ΔS° values showed that the hydrophobic interaction is the main force in the binding of Pr(III) complex and BSA. Eventually, the average aggregation number, <J>, of BSA potentially induced by title complex confirmed the 1:1 stoichiometry for title complex-BSA adducts. In vitro, antimicrobial activity of title complex was indicated that the complex is more active against both Escherichia coli and Enterococcus faecalis bacterial strains than Staphylococcus aureus, and Pseudomonas aeruginosa.
Communicated by Ramaswamy H. Sarma 相似文献
7.
Shohreh Jahani Mozhgan Khorasani-Motlagh Masoud Torkzadeh-Mahani Mahboubeh Adeli-Sardou 《Journal of biomolecular structure & dynamics》2013,31(17):4437-4449
AbstractIn this study, the interaction of Holmium (Ho) complex including 2, 9-dimethyl-1,10-phenanthroline, also called Neocuproine (Neo), [Ho(Neo)2Cl3.H2O], as fluorescence probe with fish-salmon DNA (FS-DNA) is studied during experimental investigations. Multi-spectroscopic methods are utilized to determine the affinity binding constants (Kb) of complex–FS-DNA. It is found that fluorescence of Ho complex is strongly quenched by the FS-DNA through a static quenching procedure. Under optimal conditions in Tris(trishydroxymethyl-aminomethane)–HCl buffer at 25?°C with pH?≈?7.2, intrinsic binding constant Kb of Ho complex is 6.12?±?0.04?×?105 M?1. Also, the binding site number and Stern–Volmer quenching constant are calculated. There are different approaches, including iodide quenching assay, salt effect and thermodynamical assessment to determine the features of the binding mode between Ho complex and FS-DNA. Also, the parent and starch and lipid nanoencapsulated Ho complex, as potent antitumor candidates, were synthesized. The main structure of Ho complex is maintained after encapsulation using starch and lipid nanoparticles. 3-[4,5-Dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method was used to assess the anticancer properties of Ho complex and its encapsulated forms on human cancer cell lines of human lung carcinoma cell line and breast cancer cell line. In conclusion, these compounds could be considered as new antitumor candidates.Communicated by Ramaswamy H. Sarma 相似文献
8.
The noncovalent DNA binding of the bis(1,10-phenanthroline)copper(I) complex [(Phen)2CuI] was examined under anaerobic conditions by absorption and circular dichroism spectroscopy, and viscometry, as a function of phenanthroline concentration. Analyses according to the McGhee-von Hippel method indicated that binding exhibited both neighbor-exclusion and positive cooperativity effects, with a neighbor-exclusion parameter n approximately 2 and a cooperativity parameter omega approximately 4. The association constant for (Phen)2CuI binding decreased with increasing concentration of phenanthroline in excess over that required to stoichiometrically generate (Phen)2CuI, indicating that free phenanthroline was a weak competitive inhibitor of (Phen)2CuI binding. The maximal association constant for DNA binding of (Phen)2CuI in 0.2 M NaCl and 9.8% ethanol, extrapolated to zero concentration of excess phenanthroline, was 4.7 x 10(4) M-1 (DNA base pairs). The magnitude of the neighbor-exclusion parameter, the changes in spectral properties of (Phen)2CuI induced by DNA binding, and the increase in DNA solution viscosity upon (Phen)2CuI addition are consistent with a model for DNA binding by (Phen)2CuI involving partial intercalation of one phenanthroline ring of the complex between DNA base pairs in the minor groove as suggested previously [Veal & Rill (1989) Biochemistry 28, 3243-3250]. Viscosity measurements indicated that the mono(phenanthroline)copper(I) complex also binds to DNA by intercalation; however, no spectroscopic or viscometric evidence was found for DNA binding of free phenanthroline or the bis(2,9-dimethyl-1,10-phenanthroline)copper(I) complex. DNA binding of free phenanthroline may be cooperative and induced by prior binding of (Phen)2CuI. 相似文献
9.
The oxidative addition of phenylselenium bromide to three-coordinate Pt(0) complex [Pt(2,9-dimethyl-1,10-phenanthroline)(dimethylmaleate)] affords the corresponding five-coordinate Pt(II) complex having trigonal-bipyramidal coordination geometry. The product of the reaction exists as two geometrical isomers (rotamers): in the kinetically favoured compound the olefin substituents are on the same side of the bromide ligand, while the most thermodynamically stable isomer holds the same substituents pointing at the phenylselenenide ligand. The crystal structure of the two isomers is reported and discussed with respect to the reaction mechanism and thermodynamic stability. 相似文献
10.
Wein AN Stockhausen AT Hardcastle KI Saadein MR Peng SB Wang D Shin DM Chen ZG Eichler JF 《Journal of inorganic biochemistry》2011,105(5):663-668
A gold(III) complex possessing 5,6-dimethyl-1,10-phenanthroline (5,6DMP) was synthesized and fully characterized using standard spectroscopic techniques, as well as X-ray crystallography and elemental analysis. The complex [(5,6DMP)AuCl2][BF4] (2) was found to possess a distorted square planar geometry about the gold(III) center, commonplace for d8 Au(III) cations possessing sterically un-hindered polypyridyl ligands. Compound 2 was evaluated for its potential use as an anticancer therapeutic. It was determined that the complex is stable in phosphate buffer over a 24-hour period, thought it does undergo rapid reduction in the presence of equimolar amounts of reduced glutathione (GSH) and ascorbic acid. The DNA binding and in vitro tumor cytotoxicity of the title compound 2 were also determined. It was found to undergo weak and reversible binding to calf thymus DNA, and was more cytotoxic towards a panel of human cancer cell lines than the commonly used chemotherapy agent cisplatin. Cytotoxicity experiments with the free 5,6DMP ligand indicate that the ligand has IC50 values that are slightly lower than those observed for the gold complex (2), and coupled with the fact that the ligand appears to be released from the gold(III) metal center in reducing environments, this suggests the ligand itself may play an important role in the antitumor activity of the parent gold complex. 相似文献
11.
Asieh Moodi Meissam Noroozifar Sona Niroomand 《Journal of biomolecular structure & dynamics》2013,31(8):937-950
To evaluate the biological preference of [Yb(phen)2(OH2)Cl3](H2O)2 (phen is 1,10-phenanthroline) for DNA, interaction of Yb(III) complex with DNA in Tris–HCl buffer is studied by various biophysical and spectroscopic techniques which reveal that the complex binds to DNA. The results of fluorescence titration reveal that [Yb(phen)2(OH2)Cl3](H2O)2 has strongly quenched in the presence of DNA. The binding site number n, apparent binding constant K b, and the Stern–Volmer quenching constant K SV are determined. ΔH 0, ΔS 0, and ΔG 0 are obtained based on the quenching constants and thermodynamic theory (ΔH 0?>?0, ΔS 0?>?0, and ΔG 0?<?0). The experimental results show that the Yb(III) complex binds to DNA by non-intercalative mode. Groove binding is the preferred mode of interaction for [Yb(phen)2(OH2)Cl3](H2O)2 to DNA. The DNA cleavage results show that in the absence of any reducing agent, Yb(III) complex can cleave DNA. The antimicrobial screening tests are also recorded and give good results in the presence of Yb(III) complex. 相似文献
12.
A binuclear complex [(phen)Cu(mu-bipp)Cu(phen)](ClO(4))(4), where phen=1,10-phenanthroline and bipp=2,9-bis(2-imidazo[4,5-f][1,10]phenanthroline)-1,10-phenanthroline, has been synthesized and its interaction with calf-thymus DNA in the buffer containing 5mM Tris and 50mM NaCl has been studied by means of electronic absorption titration, luminescence titration and viscometric measurements. The absorbance of the complex in the range of 320-400 nm, which is mainly based on bipp showed no obvious change upon addition of DNA, while the peak at 270 nm, which is determined by both phen and bipp decreased by up to 18%. The emission band of the complex around 360 nm decreased remarkably in presence of DNA. The emission quenching of this complex by [Fe(CN)(6)](4-) was depressed greatly when bound to DNA. The relative viscosity of DNA was increased by this complex more significantly than a bipp directed intercalating reagent. These results suggest that this complex binds to calf thymus DNA by intercalation of the two phenanthrolinecopper terminals. The apparent intrinsic binding constant of the complexes with DNA was 1.6 x 10(4)M(-1) as determined by UV-visible titration. 相似文献
13.
A novel binuclear complex [(bpy)2Ru(mu-bipp)Ru(bpy)2](ClO4)4, where bpy=2,2'-bipyridine and bipp=2,9-bis(2-imidazo[4,5-f][1,10]phenanthroline)-1,10-phenanthroline has been synthesized. Photophysical results reveal that this complex interacts with calf-thymus DNA with intrinsic binding constant 2.6 x 10(5) M(-1) in the buffer containing 5 mM Tris and 50 mM NaCl. The fact that the intraligand transition of bipp around 370 nm decreased by up to 50% in the presence of DNA, much more pronounced than the metal to ligand charge transfer band around 445 nm indicates the bridging ligand bipp is also the intercalating ligand into DNA base pairs. The emission band around at 601 nm increased by 1.4-fold, and red shifted 14 nm when DNA was added to saturation. The emission quenching of this complex by K4[Fe(CN)6] was depressed greatly when DNA was present. Viscometric measurements also proved the intercalative binding mode. 相似文献
14.
Siti Mutrofin Peter C. Healy Jean Ngoune Riccardo Pettinari Brian W. Skelton 《Inorganica chimica acta》2008,361(8):2365-2374
Syntheses, spectroscopic and structural characterizations of a series of Cu(I)-phenanthroline complexes are reported. A single crystal X-ray structure determination is recorded for CuNO3:dmp:MeCN (1:1:1), ‘dmp’ = 2,9-dimethyl-1,10-phenanthroline, showing it to be isomorphous with its previously studied tetrafluoroborate, perchlorate and hexafluorophosphate, and silver(I) perchlorate counterparts, the metal atom lying in a trigonal planar [(N∧N)Cu(NCMe)] coordination environment, the anion not being coordinated. Structure (re-) determinations are also reported for a number of salts of the [Cu(dmp)2]+ cation: the perchlorate, isomorphous with numerous other salts, not only of copper(I), but also lithium(I)), also the unsolvated nitrate, and a solvated form of the chloride. 相似文献
15.
Jiang CW 《Journal of inorganic biochemistry》2004,98(8):1399-1404
Two novel homoleptic ruthenium (II) complexes containing asymmetric tridentate ligands, 2-(benzimidazole-2-yl)-1,10-phenanthroline (PHBI) and 2-(naphtho[2,3-d]imidazole-2-yl)-1,10-phenanthroline (PHNI) have been synthesized and characterized by elemental analysis, mass spectra, (1)H NMR, and electronic spectroscopy. The DNA-binding properties of the complexes have been investigated by spectroscopic methods and viscosity measurements. The results indicate that the two complexes interact with DNA via electrostatic interaction, and the mechanisms of DNA binding with the complexes have also been discussed. 相似文献
16.
Hoi-Ling Seng Sze-Tin Von Kong-Wai Tan Mohd Jamil Maah Seik-Weng Ng Raja Noor Zaliha Raja Abd Rahman Ignez Caracelli Chew-Hee Ng 《Biometals》2010,23(1):99-118
Crystal structure analysis of the zinc complex establishes it as a distorted octahedral complex, bis(3-methylpicolinato-κ2
N,O)2(1,10-phenanthroline-κ2
N,N)-zinc(II) pentahydrate, [Zn(3-Me-pic)2(phen)]·5H2O. The trans-configuration of carbonyl oxygen atoms of the carboxylate moieties and orientation of the two planar picolinate ligands above
and before the phen ligand plane seems to confer DNA sequence recognition to the complex. It cannot cleave DNA under hydrolytic
condition but can slightly be activated by hydrogen peroxide or sodium ascorbate. Circular Dichroism and Fluorescence spectroscopic
analysis of its interaction with various duplex polynucleotides reveals its binding mode as mainly intercalation. It shows
distinct DNA sequence binding selectivity and the order of decreasing selectivity is ATAT > AATT > CGCG. Docking studies lead
to the same conclusion on this sequence selectivity. It binds strongly with G-quadruplex with human tolemeric sequence 5′-AG3(T2AG3)3-3′, can inhibit topoisomerase I efficiently and is cytotoxic against MCF-7 cell line. 相似文献
17.
DNA binding studies of ruthenium(II) complexes containing asymmetric tridentate ligands 总被引:1,自引:0,他引:1
Absorption spectroscopy, fluorescence spectroscopy and viscosity measurements have been used to characterize the DNA binding of [Ru(tpy)(dppt)](2+) (tpy=2,2':6',2"-terpyridine, dppt=3-(1,10-phenanthrolin-2-yl)-5,6-diphenyl-as-triazine), [Ru(tpy)(pta)](2+) (pta=3-(1,10-phenanthrolin-2-yl)-as-triazino[5,6-f]acenaphthylene) and [Ru(tpy)(ptp)](2+) (ptp=3-(1,10-phenanthrolin-2-yl)-as-triazino[5,6-f]-phenanthrene). The results indicate that [Ru(tpy)(pta)](2+) and [Ru(tpy)(ptp)](2+) bind with CT-DNA in an intercalative mode, while [Ru(tpy)(dppt)](2+) binds with DNA by partial intercalation. The ligand planarity of the complex has a significant effect on DNA binding affinity increases in the order [Ru(tpy)(dppt)](2+)<[Ru(tpy)(pta)](2+)<[Ru(tpy)(ptp)](2+). 相似文献
18.
The bis(1,10-phenanthroline)copper(I) complex is a relatively simple molecule previously shown to cause DNA cleavage with a strong preference for gene control regions such as the Pribnow box. Sequence level mapping of sites of [(Phen)2CuI]+ cleavage in greater than 2000 bases in histone genes and the plasmid pUC9 showed that the specificity for control regions is related to a predominant preference for minor groove binding at TAT triplets, which were cleaved most strongly at the adenosine sugar ring. The related sequences TGT, TAAT, TAGPy, and CAGT (Py = pyrimidine) were moderately preferred, while CAT and TAC triplets, PyPuPuPu quartets, PuPuPuPy quartets, and CG-rich PyPuPuPy quartets were cleaved with low to average frequency. Polypurine and polypyrimidine sequences were cleaved with low frequency. The sequence preferences of [(Phen)2CuI]+ can be ascribed predominantly to (i) a requirement for binding in the minor groove at a pyrimidine 3'----5' step and (ii) stereoelectronic effects of the 2-amino group of guanine in the minor groove, which inhibit binding. Although the reagent appears primarily to recognize sequence features at the triplet or quartet level, lower than expected cleavage was observed for two TAT sequences adjacent to several other preferred sequences and higher than expected cleavage was observed at CAAGC sequences, suggesting that longer range sequence-dependent DNA conformational effects influence specificity in certain cases. 相似文献
19.
An intercalative and minor groove binding model for the DNA cleavage reagent, copper(I) (1,10-phenanthroline)2 总被引:1,自引:0,他引:1
J C Stockert 《Journal of theoretical biology》1989,137(1):107-111
On account of the stereochemical structure and interaction characteristics of the DNA cleavage reagent copper(I) (1,10-phenanthroline)2, both intercalative and minor groove binding modes to B-DNA could be expected to occur. In the proposed model, the suitable dihedral angle between phenanthrolines allows that one of the two planar ligands partially intercalates between base pairs, and meanwhile the other ligand locates along the minor groove. 相似文献
20.
Hirohama T Kuranuki Y Ebina E Sugizaki T Arii H Chikira M Tamil Selvi P Palaniandavar M 《Journal of inorganic biochemistry》2005,99(5):1205-1219
A series of copper(II) complexes of the type [Cu(L)]2+, where L = N,N'-dialkyl-1,10-phenanthroline-2,9-dimethanamine and R = methyl (L1), n-propyl (L2), isopropyl (L3), sec-butyl (L4), or tert-butyl (L5) group, have been synthesized. The interaction of the complexes with DNA has been studied by DNA fiber electron paramagnetic resonance (EPR) spectroscopy, emission, viscosity and electrochemical measurements and agarose gel electrophoresis. In the X-ray crystal structure of [Cu(HL2)Cl2]NO3, copper(II) is coordinated to two ring nitrogens and one of the two secondary amine nitrogens of the side chains and two chloride ions as well and the coordination geometry is best described as trigonal bipyramidal distorted square based pyramidal (TBDSBP). Electronic and EPR spectral studies reveal that all the complexes in aqueous solution around pH 7 possess CuN3O2 rather than CuN4O chromophore with one of the alkylamino side chain not involved in coordination. The structures of the complexes in aqueous solution around pH 7 change from distorted tetragonal to trigonal bipyramidal as the size of the alkyl group is increased. The observed changes in the physicochemical features of the complexes on binding to DNA suggest that the complexes, except [Cu(L5)]2+, bind to DNA with partial intercalation of the derivatised phen ring in between the DNA base pairs. Electrochemical studies reveal that the complexes prefer to bind to DNA in Cu(II) rather than Cu(I) oxidation state. Interestingly, [Cu(L5)]2+ shows the highest DNA cleavage activity among all the present copper(II) complexes suggesting that the bulky N-tert-butyl group plays an important role in modifying the coordination environment around the copper(II) center, the Cu(II)/Cu(I) redox potential and hence the formation of activated oxidant responsible for the cleavage. These results were compared with those for bis(1,10-phenanthroline)copper(II), [Cu(phen)2]2+. 相似文献