Immunochemical detection of the alpha-subunit of the G-protein, GZ, in membranes and cytosols of mammalian cells

An antiserum (AS 98) was raised against a synthetic peptide deduced from published cDNA sequences of the alpha-subunit of the putative G-protein, GZ (Fong et al. Proc. Natl. Acad. Sci. USA 85, 3066-3070, 1988; Matsuoka et al. Proc. Natl. Acad. Sci. USA 85, 5384-5388, 1988). In membrane and cytosol preparations of many but not all tested mammalian tissues, AS 98 predominantly recognized two proteins of 40 and 43 kDa Mr. Whereas high levels of a 40 kDa GZ alpha-subunit were found in rat liver membranes and in brain cytosol, AS 98 failed to detect the alpha-subunit of GZ in brain membranes.     

Mechanistic insights into the action of Bisphenol A on the germline using C. elegans

Feature on: Allard P, et al. Proc Natl Acad Sci USA 2010; 107:20405-10.     

Antiangiogenic agents

Comment on: Conley SJ, et al. Proc Natl Acad Sci USA 2012; In press     

Manipulation of single polymerase-DNA complexes: A mechanical view of DNA unwinding during replication

Comment on: Morin JA, et al. Proc Natl Acad Sci USA 2012; 109:8115-20.     

Role for Geminin in sustaining the activity of hematopoietic stem cells

Ohno Y, et al. Proc Natl Acad Sci USA 2010; In press.     

Lateral diffusion in inhomogeneous membranes. Model membranes containing cholesterol.

The problem of lateral diffusion in inhomogeneous membranes is illustrated by a theoretical calculation of the lateral diffusion of a fluorescent lipid probe in binary mixtures of phosphatidylcholine and cholesterol under conditions of temperature and composition such that this lipid mixture consists of alternating parallel domains of fluid and solid lipid, having separations that are small compared with the distance scale employed in photobleaching experiments. The theoretical calculations clearly illustrate how inhomogeneities in membrane composition affecting the lateral motion of membrane components on a small (10-100 nm) distance scale can give complex diffusive responses in experiments such as fluorescence photobleaching that employ comparatively macroscopic distances (10-100 micrometers) for the measurement of diffusive recovery. The theoretical calculations exhibit the unusual dependence of the apparent lateral diffusion coefficient of a fluorescent lipid probe on lipid composition in binary mixtures of cholesterol and phosphatidylcholines as reported by Rubenstein et al. (1979, Proc. Natl. Acad. Sci. U.S.A., 76:15-18).     

Developmental relationships between B-1 and B-2 progenitors

Comment on: Barber CL, et al. Proc Natl Acad Sci USA 2011; In press.     

The role of eIF5A in protein synthesis

Comment on: Henderson A, et al. Proc Natl Acad Sci USA 2011; 108:6415-9.     

MicroRNA-dependent regulation of the microenvironment and the epithelial stromal cell interactions in the mouse mammary gland

Ohno Y, et al. Proc Natl Acad Sci USA 2010; In press.     

Changing pace: Viral mimicry of an anaphase promoting complex subunit

Comment on: Mo M, et al. Proc Natl Acad Sci USA 2009; 106:19527-32.     

Probing the complexity of miRNA expression across hematopoiesis

Comment on: Petriv OI, et al. Proc Natl Acad Sci USA 2010; 107:15443-8.     

Recognizing the cooperative and independent mitochondrial functions of Parkin and PINK1

Comment on: Johnson BN, et al. Proc Natl Acad Sci USA 2012; 109:6283-8.     

Mismatch repair outside of replication

Comment on: Rodriguez GP, et al. Proc Natl Acad Sci USA 2012; 109:6153-8.     

Lack of communication rusts and ages stem cells

Comment on: Ishikawa ET, et al. Proc Natl Acad Sci USA 2012; 109:9071-6.     

Sibling rivalry in checkpoint control of cell cycle and DNA damage response

Comment on: Pabla N, et al. Proc Natl Acad Sci USA 2012; 109:197-202.     

Revisited function of human NK cell subsets

Comment on: De Maria A, et al. Proc Natl Acad Sci USA 2011; 108:728-32.     

Recognizing the cooperative and independent mitochondrial functions of Parkin and PINK1

Comment on: Johnson BN, et al. Proc Natl Acad Sci USA 2012; 109:6283-8.     

Translating niche-derived signals into neurogenesis: The function of Prox1 in the adult hippocampus

Comment on: Karalay O, et al. Proc Natl Acad Sci USA 2011; 108:5807-12.     

Connection between induction of DNA lesions and DNA recombination/repair during Ig class switch recombination

Comment on: Kracker S, et al. Proc Natl Acad Sci USA 2010; 107:22225-30.