共查询到20条相似文献,搜索用时 31 毫秒
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Large-scale statistical analysis of secondary xylem ESTs in pine 总被引:3,自引:0,他引:3
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Transcriptome sequencing and comparative analysis of the gametophyte thalli of Pyropia tenera under normal and high temperature conditions 总被引:1,自引:0,他引:1
San Choi Mi Sook Hwang Sungoh Im Namju Kim Won-Joong Jeong Eun-Jeong Park Yong-Gun Gong Dong-Woog Choi 《Journal of applied phycology》2013,25(4):1237-1246
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Construction of a full-length cDNA Library from Chinese oak silkworm pupa and identification of a KK-42-binding protein gene in relation to pupa-diapause termination
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Yu-Ping Li Run-Xi Xia Huan Wang Xi-Sheng Li Yan-Qun Liu Zhao-Jun Wei Cheng Lu Zhong-Huai Xiang 《International journal of biological sciences》2009,5(5):451-457
In this study we successfully constructed a full-length cDNA library from Chinese oak silkworm, Antheraea pernyi, the most well-known wild silkworm used for silk production and insect food. Total RNA was extracted from a single fresh female pupa at the diapause stage. The titer of the library was 5 × 105 cfu/ml and the proportion of recombinant clones was approximately 95%. Expressed sequence tag (EST) analysis was used to characterize the library. A total of 175 clustered ESTs consisting of 24 contigs and 151 singlets were generated from 250 effective sequences. Of the 175 unigenes, 97 (55.4%) were known genes but only five from A. pernyi, 37 (21.2%) were known ESTs without function annotation, and 41 (23.4%) were novel ESTs. By EST sequencing, a gene coding KK-42-binding protein in A. pernyi (named as ApKK42-BP; GenBank accession no. FJ744151) was identified and characterized. Protein sequence analysis showed that ApKK42-BP was not a membrane protein but an extracellular protein with a signal peptide at position 1-18, and contained two putative conserved domains, abhydro_lipase and abhydrolase_1, suggesting it may be a member of lipase superfamily. Expression analysis based on number of ESTs showed that ApKK42-BP was an abundant gene in the period of diapause stage, suggesting it may also be involved in pupa-diapause termination. 相似文献
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Ali N Dana Maureen E Hillenmeyer Neil F Lobo Marcia K Kern Patricia A Romans Frank H Collins 《BMC genomics》2006,7(1):1-24
Background
Large scale sequencing of cDNA libraries can provide profiles of genes expressed in an organism under defined biological and environmental circumstances. We have analyzed sequences of 4541 Expressed Sequence Tags (ESTs) from 3 different cDNA libraries created from abdomens from Plasmodium infection-susceptible adult female Anopheles gambiae. These libraries were made from sugar fed (S), rat blood fed (RB), and P. berghei-infected (IRB) mosquitoes at 30 hours after the blood meal, when most parasites would be transforming ookinetes or very early oocysts.Results
The S, RB and IRB libraries contained 1727, 1145 and 1669 high quality ESTs, respectively, averaging 455 nucleotides (nt) in length. They assembled into 1975 consensus sequences - 567 contigs and 1408 singletons. Functional annotation was performed to annotate probable molecular functions of the gene products and the biological processes in which they function. Genes represented at high frequency in one or more of the libraries were subjected to digital Northern analysis and results on expression of 5 verified by qRT-PCR.Conclusion
13% of the 1965 ESTs showing identity to the A. gambiae genome sequence represent novel genes. These, together with untranslated regions (UTR) present on many of the ESTs, will inform further genome annotation. We have identified 23 genes encoding products likely to be involved in regulating the cellular oxidative environment and 25 insect immunity genes. We also identified 25 genes as being up or down regulated following blood feeding and/or feeding with P. berghei infected blood relative to their expression levels in sugar fed females. 相似文献10.
Development of ESTs and data mining of pineapple EST-SSRs 总被引:1,自引:0,他引:1
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Alexandre Augusto Pereira Firmino Fernando Campos de Assis Fonseca Leonardo Lima Pepino de Macedo Roberta Ramos Coelho José Dijair Antonino de Souza Jr Roberto Coiti Togawa Orzenil Bonfim Silva-Junior Georgios Joannis Pappas-Jr Maria Cristina Mattar da Silva Gilbert Engler Maria Fatima Grossi-de-Sa 《PloS one》2013,8(12)
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