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1.
V S Khristoforov V M Persanov A G Sukhomudrenko V M Abramov V P Zav'ialov 《Biofizika》1987,32(1):32-36
A high degree of correlation between the capability of subclasses of human immunoglobulins G to form aggregates due to thermal treatment, and their complement-binding activity was established. On the basis of the experimental data obtained by the methods of light scattering, circular dichroism, microcalorimetry, it was supposed that "hinge" region of immunoglobulins G participates in the initial stage of thermal aggregation and in the activation of the process of complement binding. 相似文献
2.
The isolation of guinea-pig immunoglobulins γ1G, γ2G and γM are described and methods for separating the polypeptide chains of each examined. The molecular weights, extinction coefficients and carbohydrate and amino acid compositions of the immunoglobulins and their constituent chains have been analysed. The findings provide a basis for further studies attempting to relate structural differences to distinct biological properties of guinea-pig immunoglobulins. 相似文献
3.
The modification of human immunoglobulin binding to staphylococcal protein A using diethylpyrocarbonate 总被引:1,自引:0,他引:1
D A Haake E C Franklin B Frangione 《Journal of immunology (Baltimore, Md. : 1950)》1982,129(1):190-192
Human IgG subclasses 1, 2, and 4, as well as proteins of the IgG3 subclass that are allotype G3m (s+t+), bind avidly to staphylococcal protein A by means of their Fc portion. Proteins of the IgG3 subclass that are allotype G3m (s-t-) do not bind. The importance of a histidine residue at position 435 has been implicated from comparison of amino acid sequences of immunoglobulins that bind with those that do not bind to staphylococcal protein A, as well as from crystallographic data. Modification of histidines at a low concentration of diethylpyrocarbonate successfully and reversibly alters the binding of immunoglobulins to staphylococcal protein A with only minimal change in the antigenic properties. This method provides strong evidence for the critical importance of histidine in the binding of immunoglobulins to staphylococcal protein A. 相似文献
4.
V S Khristoforov V P Kutyshenko V M Abramov V P Zav'ialov 《Bioorganicheskaia khimiia》1986,12(11):1469-1477
Conformational properties of the Fc- and pFc'-fragments of human myeloma immunoglobulins G of the first and third subclasses were studied by 1H-NMR method (270 and 400 MHz). It was found that the globular structures (domains) of the Fc-fragments of IgG1 and IgG3 in solution are characterized by high segmental mobility, and have no significant differences in their spatial arrangement. Comparative analysis of the spectra obtained at different temperatures (30-70 degrees C) revealed that the Fc-fragment of IgG3 has a more heat-stable conformation than the Fc of IgG1. The intramolecular mobility of the Fc-fragment increased upon lowering the pH. The partial assignment of the signals in the NMR spectra of the Fc-fragments of immunoglobulins G1 and G3 was carried out, and the pKa values for histidines of the pFc'-fragment of IgG1 were determined. 相似文献
5.
Jae Hong Im Takashi Nakane Hiroshi Yanagishita Toru Ikegami Dai Kitamoto 《BMC biotechnology》2001,1(1):5-7
Background
There have been many attempts to develop new materials with stability and high affinity towards immunoglobulins. Some of glycolipids such as gangliosides exhibit a high affinity toward immunoglobulins. However, it is considerably difficult to develop these glycolipids into the practical separation ligand due to their limited amounts. We thus focused our attention on the feasible use of "mannosylerythritol lipid A", a yeast glycolipid biosurfactant, as an alternative ligand for immunoglobulins, and undertook the investigation on the binding between mannosylerythritol lipid A (MEL-A) and human immunoglobulin G (HIgG). 相似文献6.
Fibronectin isolated from bovine serum by affinity chromatography on collagen-Sepharose was found to contain a great number of concomitant proteins. Polyacrylamide gel electrophoresis of experimental samples pretreated with beta-mercaptoethanol under denaturation conditions resulted in the polypeptide fractions with Mr of 25, 54 and 82 KD, while the non-treated samples contained only one protein of non-fibronectin type (Mr = = 180-190 KD). This protein was isolated from the total preparations of collagen-binding proteins by the procedures generally employed for the isolation of purified preparations of immunoglobulins G; this protein was also isolated from purified immunoglobulins G using affinity chromatography on collagen-Sepharose. In terms of its molecular weight, subunit composition and immunological and chromatographical behaviour this protein can be related to immunoglobulins. The immunoglobulin-like protein isolated together with fibronectin revealed an affinity for denatured collagen, but not for fibronectin or Sepharose. The content of immunoglobulin with an affinity for denatured collagen in the total fraction of immunoglobulins G is 0.3-0.5%. 相似文献
7.
J Ka?uza J Stachura E Junikiewicz 《Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society》1985,23(4):261-266
The localization of immunoglobulins G, A and M in glial cells of neoplastic and reactive origin have been investigated by the use of the PAP (peroxidase-antiperoxidase) method on paraffin embedded tissue previously fixed in calcium formol. It has been found, that some glial cells of astrocyte type showed a very intense staining when oligoclonal antibodies to human immunoglobulins G, A and M specific for gamma, alpha, and mu chains were used. The localization of immunoglobulins was disclosed in astrocytes of various morphology; astrocytes with well developed processes, gemistocyte type cells without or only with short and thick cell processes and in small cells with scanty cytoplasm. The number of cells with immunoglobulins localized is very small. No positive results have been noted if the normal brain tissue is concerned. The specificity of the method is discussed. 相似文献
8.
Proteins capable of non-immune binding of immunoglobulins G (IgG) of various mammalian species, i.e. without the involvement
of the antigen-binding sites of the immunoglobulins, are widespread in bacteria. These proteins are located on the surface
of bacterial cells and help them to evade the host’s immune response due to protection against the action of complement and
to decrease in phagocytosis. This review summarizes data on the structure of immunoglobulin-binding proteins (IBP) and their
complexes with IgG. Common and distinctive structural features of IBPs of gram-positive bacteria (staphylococci, streptococci,
peptostreptococci) are discussed. Conditions for IBP expression by bacteria and their functional heterogeneity are considered.
Data on IBPs of gram-negative bacteria are presented. 相似文献
9.
Post-translational processing of chicken bone phosphoproteins. Identification of the bone phosphoproteins of embryonic tibia. 总被引:1,自引:1,他引:0
After periodate oxidation and incubation with a dihydrazide, cross-linking of the two heavy chains of immunoglobulins G from several species proceeds specifically through their oligosaccharides. We have used malonic acid dihydrazide, adipic acid dihydrazide and dithiodipropionic acid dihydrazide. The last compound is introduced in this work as a cleavable-carbohydrate-specific cross-linker. It was found that in rabbit and human immunoglobulins the degree of cross-linking was strongly dependent on the oxidation conditions but only very weakly dependent on the concentration and size of the dihydrazides. Papain cleavage of the cross-linked rabbit IgG indicated that the cross-linking occurred predominantly, if not exclusively, in the Fc region, probably through the two glycans linked to Asn-297 in the CH2 domain of each of the two heavy chains. The immunoglobulins from sheep, pig, goat and guinea pig show a comparable cross-linking pattern, indicating that the sugar chains from these immunoglobulins have a spatial structure closely related to that of rabbit and human IgG. When dithiodipropionic acid dihydrazide was used as the cross-linker, the cross-link could be cleaved by mercaptoethanol. 相似文献
10.
R. C. Richards 《The Histochemical journal》1984,16(5):565-572
Summary Frozen sections, 0.5 m thick, of the lactating mouse mammary gland have been used to localize immunoglobulins A and G and serum albumin throughout the connective tissue stroma, in the lumina of blood vessels, in milk stored in the alveoli and in the lateral spaces between adjacent epithelial cells. In addition, the immunoglobulins were localized to their specific plasma cells in the connective tissue stroma. Serum albumin was further identified within the mammary epithelial cells as small spots of fluorescence scattered throughout the cytoplasm. The immunoglobulins were not localized within these cells in untreated sections, but in sections treated with trypsin and Soybean trypsin inhibitor, it was possible to identify a similar distribution to that for serum albumin. The spots of fluorescence representing the intracellular localization of the immunoglobulins and serum albumin were frequently found in association with the periphery of intracellular lipid droplets. 相似文献
11.
Levashov PA Ovchinnikova ED Afanas'eva MI Frid DA Al'muko AA Bespalova ZhD Adamova IIu Afanas'eva OI Pokrovskiĭ SN 《Bioorganicheskaia khimiia》2012,38(1):58-63
New chromatographic material based on tryptophil-threonil-tirosine was prepared. This sorbent effectively binds human, sheep, goat and cow immunoglobulins G. New sorbent shows high selectivity for removing immunoglobulins from blood plasma. Effective sorption capacity is 15-25 mg of immunoglobulin G per ml of matrix. Optimal method of covalent attachment ligand to polysaccharide matrix allows achieving high stability of the sorbents in terms of use and storage. This sorbent can be used in medicine and biotechnology. 相似文献
12.
1. The products from papain and pepsin hydrolyses of the guinea-pig immunoglobulins gamma(1)G and gamma(2)G were isolated and characterized with regard to molecular weight, amino acid composition, hexose content and antigenic specificity. 2. Fragments Fab and (Fab')(2) from immunoglobulins gamma(1)G and gamma(2)G have similar electrophoretic and antigenic properties, but show some class-specific differences in amino acid composition. 3. Three Fc fragments were obtained after papain digestion of immunoglobulin gamma(2)G, namely, fragment Fc dimer (mol.wt. 58000), fragment Fc monomer (mol.wt. 29000) and fragment Fc' (mol.wt. 8000). A single crystalline fragment, namely fragment Fc' (mol.wt. 11000), was isolated after papain digestion of immunoglobulin gamma(1)G. 4. Peptic digestion of immunoglobulins gamma(1)G and gamma(2)G releases C-terminal fragments, namely, fragments pFc', of similar molecular weight (13000) but different amino acid compositions and distinct antigenic specificities. 5. Digestion-time studies show that immunoglobulin gamma(1)G is far more susceptible to proteolysis than is immunoglobulin gamma(2)G and suggest that at least a proportion of molecules are split primarily at a site that liberates fragment gamma(1)Fc'. 相似文献
13.
E V Senchurin V P Korotkoruchko E I Cherniavaski? 《Ukrainski? biokhimicheski? zhurnal》1978,50(3):352-356
Blood proteins were examined in rats with the Shvets experimental leukosis positively reacting in the sedimentation test for leukosis. Application of monospecific antisera against main classes of immunoglobulins shows that this protein belongs to immunoglobulins of the class G. The sedimentation constant and molecular weight of the protein under study are determined. It is shown that immunoglobulin G of rats with leukosis activates glycolysis and inhibits respiration. The data obtained give reasons to assume not complete identity but, in any case, similarity of physicochemical properties of proteins appearing in animal and human blood channel with malignant growth. 相似文献
14.
《Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology》1983,742(2):295-302
Conformational properties of human myeloma immunoglobulins G belonging to four subclasses (IgG1 Van, IgG2 Kom, IgG3 Pla, IgG4 Ang), and also Fab, Fc and pFc′ fragments derived from IgG1 Van, IgG2 Kom and IgG3 Pla have been studied by temperature-perturbation and solvent-perturbation spectroscopy. It has been shown that the immunoglobulins studied practically do not differ in the number of tyrosine and tryptophan residues exposed to different solvent perturbants (saccharose, glycerol, dimethylsulfoxide). The same regularity is observed for isolated Fab and Fc fragments. At the same time, the immunoglobulins compared and their proteolytic fragments significantly differ in the number of aromatic chromophores perturbed by temperature. These data indicate that immunoglobulins of different subclasses and their subunits have a different rigidity of structure in relation to thermal perturbation. The Fc subunits of IgG1 are characterized by the lowest rigidity of structure of internal hydrophobic cores of domains (characterized by the rigidity of the microenvironment of tryptophan residues), as compared with the Fc subunits of IgG2 and IgG3. In the case of IgG1 and IgG2, these differences seem to be brought about by a different rigidity of structure of CH2 domains, since thermal-perturbation spectra of the pFc′ fragments of these subclasses practically coincide. The total number of chromophores exposed to different solvent perturbants in the isolated Fab and Fc fragments practically coincides with the number of exposed chromophores in intact immunoglobulins. Similar coincidence is observed for the tryptophan residues perturbed by temperature. These data indicate that neither the conformation of surface sites nor the conformation of internal hydrophobic cores of domains significantly changes on isolation of Fab and Fc fragments. At the same time, many more tyrosine residues are perturbed by temperature in the intact immunoglobulin G1 Van than in the corresponding sum of isolated Fab and Fc fragments, while for IgG2 Kom, which has the same length of hinge region, these values practically coincide. This fact can be explained by the greater temperature dependence of motions of subunits in IgG1 Van as compared with IgG2 Kom, and as a result of this by the higher mutual temperature-dependent influence of subunits on their internal structure (on interdomain interactions). 相似文献
15.
Kostiushov VV Tymchishin OL Kutkovets SL Vitenskiĭ VS Kostiushova NV Ratushnenko VA 《Ukrainski? biokhimicheski? zhurnal》2002,74(1):62-70
The interaction of blood serum immunoglobulins of M, G, and A classes of the donors with monospecific serums (MSS)-anti-IgM, anti-IgG and anti-IgG was established to be associated by Ag(+)-sensitive-SH containing non protein compounds release. This phenomenon formation should be related to a parallel running associated reaction mediated by conformational and/or some other changes of immunoglobulins macrostructure under highly specific intermolecular interaction with adequate MSS in the reactive mixtures. As a rule these processes are associated by the break and reduction of mixed disulphide bounds between thiol containing nonprotein compounds and proteins. HIV antigen glycoproteins and morphine preliminary introduced into the analogic reactive mixtures were found to block this phenomenon. If in these reactive mixtures the serums including three serotypes hepatitis B virus antigen is introduced this phenomenon is preserved. This effect of HIV antigen glycoproteins and morphine could be explained by their direct and/or mediated influence on the immunoglobulins macrostructure. As a result of the latter the immunoglobulins structure-functional status is infringed, being indirectly evidenced by absence of the associated reaction of release Ag(+)-sensitive-SH containing non protein compounds in the reactive mixtures. The processes presented are capable to play an essential role in formation of polyclonal gammapathy under HIV-infection. 相似文献
16.
V D Golovko N I Tsirel'nikov I N Ozhiganova V G Koptelov 《Arkhiv anatomii, gistologii i émbriologii》1991,101(11-12):62-68
An investigation of 88 placentas in toxicosis of the second half of pregnancy has detected symptoms of the immune alteration detailed by means of an electron microscopic analysis and immunomorphological investigations with the help of luminescent sera against immunoglobulins A, M. G and C3 fraction of complement. The alterations found are indicative of the participation of immune mechanisms in the formation of placental insufficiency and their important role in pathogenesis of gestational toxicosis. 相似文献
17.
A P Fedorova E G Kostrzhevskaia B A Protsenko 《Ukrainski? biokhimicheski? zhurnal》1978,50(3):307-310
The total amount of carbohydrates and some carbohydrate components was studied in total preparations of immunoglobulins of blood serum of healthy people and cancer patients as well as in immunoglobulin G subfraction peculiar to cancer and in the fraction isolated from immunoglobulin G of healthy people blood serum corresponding to the place of column elution. An insignificant increase is established in the content of carbohydrates in the protein peculiar to cancer as compared to their amount in immunoglobulin G of blood serum from healthy people (1.93 and 1.46 g per 100 g of protein, respectively). A conclusion is drawn that the content of the studied substances in the protein peculiar to cancer cannot determine the peculiarities of its physicochemical, immunological and biological properties. 相似文献
18.
Yingying Su Haidong Wei Yanju Bi Yanan Wang Peng Zhao Runxiang Zhang Xiang Li Jianhong Li Jun Bao 《Journal of cellular physiology》2019,234(5):7198-7212
Acclimation can alleviate the damage caused by adverse environmental factors. To investigate the effects of cold stimulation on immunity in tracheal of broilers, 360 one-day-old chicks were raised at normal temperatures during 1–7 days. From 8 day, G1 (control) continued to be raised at normal temperatures, whereas G2 and G3 (treatment groups) were cold-stimulated at 3°C and 12°C below the temperature of G1, respectively. At 42 day, all the groups were subjected to a 24-hr acute cold stress, designated as S1, S2, and S3. Tracheal tissues were collected to detect gene levels of immunoglobulins, antimicrobial peptides, Hsps, and cytokines, and oxidative stress-related indicators at 14 day, 42 day, and 43 day, and protein levels of Hsps and proinflammatory cytokines as well as morphology changes at 42 day and 43 day. The results showed that, compared with 42G1, tracheal structure of 42G2 was basically intact, and gene levels of immunoglobulins and antimicrobial peptides increased (p < 0.05), whereas tracheal structure of 42G3 was destroyed, with decreased levels of immunoglobulins ( p < 0.05), and increased levels of Hsps and proinflammatory cytokines ( p < 0.05). At 43 day, tracheal damage was visible and gene levels of immunoglobulins and antimicrobial peptides decreased in S1 ( p < 0.05). Tracheal structure was relatively intact and gene levels of antimicrobial peptides increased in S2 ( p < 0.05). Compared with S1 and S3, immune-related gene levels in S2 were higher, and Hsps and proinflammatory cytokines levels were lower. The results demonstrate that cold stimulation of lower 3°C from 8 to 42 day led to cold acclimation, which improved immunity of tracheal mucosa and resistance to cold stress in broilers. 相似文献
19.
R L Panurina K L Shakhanina V S Zhuravlev E M Gavrilova 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1978,(4):67-73
Sera of 86 patients suffering from G-myeloma were studied for the purpose of determination of subclasses of monoclone IgG. Investigations were carried out by means of antisera to subclasses IgG by the double diffusion method in gel after Ouchterlony. The following distribution of myeloma Ig was revealed: G1--70%, G2--17%, G3--11%,and G4--2%. In typing of the light igG chains by the method of immunoelectrophoresis, using antisera to the light chains of immunoglobulins of the chi and lambda type it was found that IgG1 chi was encountered more frequently than IgG1 lambda (3:1 ratio). The amount of the sera with the IgG2, IgG3, and IgG4 was insufficient for the reliable conclusion of their distribution by the type of light chains. 相似文献
20.
Lv YX Zhao SP Zhang JY Zhang H Xie ZH Cai GM Jiang WH 《International journal of biological macromolecules》2012,50(4):1144-1150
The processing parameters of pump speed, inlet air temperature, outlet air temperature and homogenization pressure were evaluated. Encapsulation efficiency is high with a satisfied releasing rate. Then, acute otitis media (AOM) animal model was built and diet containing orange peel essential oil microcapsules were administrated to AOM animals. Pharmacological test showed that orange peel essential oil treatment could decrease serum and cochlea malondialdehyde (MDA), immunoglobulins A (IgA), immunoglobulins G (IgG), immunoglobulins M (IgM) levels and increase antioxidant enzymes activities. It can be concluded that orange peel essential oil treatment could decrease oxidative injury in acute otitis media rats. 相似文献