Interspecific competition between two field populations of grass-feeding bugs

Abstract. 1. We tested the hypothesis that interspecific competition between two grass-feeding stenodemine bug species ( Notostira elongata Geoffroy and Megaloceraeu recticornis Geoffroy) was capable of causing population-scale mortality in the field.
2. N.elongata nymphs were added to two field enclosures in which the M.recti-cornis population was to hatch. In one of these enclosures the grass Arrhena-therum elatius L., on which only M.recticornis can feed, was also added. Population changes of the bug species were then monitored. An unmanipulated enclosure and an unfenced plot of grassland were used as controls.
3. The results demonstrated that N.elongata lowered the survival of M.recticornis and that this effect was removed by the addition of A.elatius . The results also suggested that N.elongata suffered less from the effects of competition with M.recticornis when the latter had access to the refuge foodplant.
4. Interspecific competition was rare among members of the grass-feeding stenodemine guild in the area studied. Only one out of fifteen possible pairs competed, and this competition occurred only under special circumstances.     

Fire effects on insect herbivores in an oak savanna: the role of light and nutrients

Abstract.  1. Environmental heterogeneity created by prescribed burning provided the context for testing whether the distribution of an oak specialist (the lace bug, Corythuca arcuata ) could be explained by stoichiometric mismatches between herbivore and host plant composition.
2. Field observations showed that lace bug density was seven-fold higher in frequently burned than in unburned units.
3. Lace bug density did not increase with leaf nutrient concentrations, but was instead associated with higher light levels, higher concentrations of leaf carbon (C), lignin and total phenolics, and lower levels of cellulose. In addition, lace bugs reared on high-light leaves had higher levels of survivorship than those fed on low-light leaves.
4. Sampling restricted to full-sun leaves was used to test whether fire-related changes in leaf nitrogen (N) and phosphorus (P) concentrations have a secondary influence on lace bug success. This sampling provided only limited evidence for nutrient limitation, as decreases in leaf N and P were associated with an increase in lace bug mass but a decrease in density.
5. It is concluded that burning probably promotes lace bug population growth by increasing canopy openness, light penetration, and the availability of C-based metabolites, and thus simple stoichoimetric mismatches between herbivores and host plants are not of primary importance in this system.     

Niche use patterns among some Stenodemini (Heteroptera: Miridae) of limestone grassland,and an investigation of the possibility of interspecific competition between Notostira elongata Geoffroy and Megaloceraea recticornis Geoffroy

Summary It was suggested in a previous paper that mortality patterns in two species of Stenodemini (M. recticornis and N. elongata) could be explained by interspecific competition (Gibson 1976a)Such competition would have been generated by seasonal changes in the foodplants used by the two species resulting in both species using virtually the same range of plants at one time of year.As with a number of phytophagous insects (McNeill and Southwood 1978) many changes in foodplant use could be explained by differential seasonal changes in nitrogen content of the plant part (leaves) that the bugs were using in different plant species. Each bug had an ideal food nitrogen range which it used whenever possible. Lack of fit to this mechanism could often be explained by defensive chemistry and physical properties of some grass species.Although the total bug population was taking under 1% of the area's primary production, a potential refuge foodplant for one bug species was very heavily eaten where it was rare, and behavioural interference between bug species suggested that space to feed in was a limiting resource.Laboratory and semi-field culture experiments showed that the presence of one bug species adversely affected the growth and/or survival of the other, although the outcome of competition was different to that in the field, probably due to the different relative and absolute densities used.Although one cannot be completely certain without field manipulation experiments, it is extremely likely that interspecific competition was occurring and could be stabilised by the losing species (N. elongata) having a refuge generation.Competition between stenodemine species on the study area could usually be avoided by separation along a number of resource axes e.g. foodplant species, plant part, nitrogen level of food or emergence timing. In the present circumstance, a particular combination of bug and plant species in the area has forced one species pair into overlap. It should be possible to predict the guild structure and competition relations in other areas from this.     

The influence of feeding on predatory tactics in a water bug

Abstract. The tropical belostomatid Diplonychus indicus Venk. & Rao (Heter-optera: Belostomatidae) forages both actively and from ambush, changing frequently from one predatory mode to the other. Analyses presented here of the influence of feeding and of developmental stage on several locomotory and predatory parameters show that the fact that a bug was eating did not prevent it from reacting to and from seizing prey items, but it did influence the mode of predatory attempts. Feeding bugs were less active and they foraged actively less frequently, but feeding did not affect the frequency of predatory attempts from ambush. No important variations during development were found and first stadium larvae behaved similarly to adults. Capture success rates were higher at all developmental stages during a meal than when a bug was not eating and rejection rates were lower during a meal. These results suggest that, when eating, Diplonychus indicus bugs aim more successfully at prey items they are more likely to capture and to eat.     

荔枝蝽取食行为的研究

在越冬前和产卵期,荔枝蝽Tessaratoma papillosa Drury在寄主植物-荔枝树的花枝、嫩枝和老枝上都有取食行为。但在这两个不同时期, 其取食行为有显著差异。在越冬前,只有50%～70%的成虫在各类枝条上取食;而处于产卵期的荔枝蝽成虫,在各类枝条上,100%积极取食,且其取食前时间显著短于越冬前在同类枝条上的取食前时间。同一时期在不同枝叶上,荔枝蝽的取食行为也有显著差异。被置于嫩枝叶和花枝上的成虫取食前时间明显短于被置于老枝叶上者;在有选择的情况下,两个时期的成虫都明显地选择在花枝和嫩枝上取食。经分析测定：在不同生长时期,荔枝树的花枝和嫩枝的含水量与含氮量均较老枝叶中的高,可溶性糖含量的变化较大;组织结构也存在明显差异。     

Host plant toxins and unpalatability of Neacoryphus bicrucis (Hemiptera: Lygaeidae)

Abstract. 1. Host plant choice of the seed bug, Neacoryphus bicrucis Say (Hemiritera, Lygaeidae), was evaluated in four different Georgia habitats. N. bicrucis utilized only species of Senecio as host plants at a granite outcrop and at old fields on the coastal plain, in the piedmont, and in the Blue Ridge Mountains.
2. In laboratory tests N. bicrucis is distasteful to the green anole lizard, Anolis carolinensis Voight. Only six of ninety field-collected bugs were eaten in palatability trials while eighty-four of the remaining ninety attacked bugs were rejected by lizards without apparent harm. However, the bugs were palatable to Fowler's toads, Bufo woodhousei fowleri Hinckley, which ate all thirty-six bugs offered to them.
3. N. bicrucis selectively sequesters pyrrolizidine alkaloids from Senecio spp. These may cause the insects' distastefulness to green anoles. In palatability trials, lizards ate all twenty insects reared on sunflower seeds, Helianthus annus, but rejected all twenty reared on Senecio smallii.     

Composition and abundance of stink bugs (Heteroptera: Pentatomidae) in corn

The species composition and abundance of stink bugs (Heteroptera: Pentatomidae) in corn, Zea mays L., was determined in this on-farm study in Georgia. Seven species of phytophagous stink bugs were found on corn with the predominant species being Nezara viridula (L.) and Euschistus servus (Say). All developmental stages of these two pests were found, indicating they were developing on the corn crop. The remaining five species, Oebalus pugnax pugnax (F.), Euschistus quadrator (Rolston), Euschistus tristigmus (Say), Euschistus ictericus (L.), and Acrosternum hilare (Say), were found in relatively low numbers. Adult N. viridula were parasitized by the tachinid parasitoid Trichopoda pennipes (F.). There was a pronounced edge effect in distribution of stink bugs in corn. Population dynamics of N. viridula and E. servus were different on early and late-planted corn. Oviposition by females of both stink bug species occurred in mid-to-late-May and again mid-to-late-June in corn, regardless of planting date. In early planted fields, if stink bug females oviposited on corn in mid-July, the resulting nymphs did not survive to the adult stage in corn because ears were close to physiological maturity and leaves were senescing. Density of stink bug adults in early planted corn was relatively low throughout the growing season. In late-planted corn, females of both stink bug species consistently laid eggs in mid-to-late-July on corn with developing ears. This habitat favored continued nymph development, and the resulting adult population reached high levels. These results indicate that corn management practices play a key role in the ecology of stink bugs in corn agroecosystems and provide information for designing management strategies to suppress stink bugs in farmscapes with corn.     

The influence of host plant diversity and food quality on larval survival of plant feeding heteropteran bugs

Abstract. 1. In a laboratory experiment, the influence of host plant diversity and food quality, in terms of nitrogen content, on the larval survival of two oligophagous bug species (Heteroptera, Miridae: Leptopterna dolobrata L., Notostira erratica L.) was investigated. Both species are strictly phytophagous and capable of feeding on a wide range of grass species. Moreover, they typically change their host plants during ontogenesis; it has been suggested that this behaviour is a response to the changing protein content of the hosts.
2. To investigate the importance of host plant diversity for these insects, the development of insects reared on grass monocultures was compared with that on mixtures of four grass species. In addition, the host grasses were grown under two nitrogen regimes to test whether nitrogen content is the key factor determining host plant switching.
3. Both species had a significantly higher survival rate when feeding on several host plants but only L. dolobrata showed a significant response to food nitrogen content. Furthermore, there was no correlation between the nitrogen content of the host plants and the survival rate of N. erratica larvae.
4. The study suggests that at least some Stenodemini need a variety of host plants during larval development but that the level of host plant nitrogen is not the main factor responsible for the observed diversity effect.     

Evaluation of the impacts of herbivory by lace bugs on Chinese privet (Ligustrum sinense) survival and physiology

Biological control of Chinese privet, Ligustrum sinense, is the best long-term option for control of this widespread invasive plant in the southeastern USA. A pre-release efficacy assessment was conducted by testing the effects of damage caused by a lace bug, Leptoypha hospita, on potted privet plants in the laboratory. Inoculating 15 pairs of lace bug adults on plants resulted in a significantly higher defoliation rate and reduced leaf biomass by more than 59% compared to 0 and 3 lace bug pairs. Leaf biomass of plants inoculated with 3 and 9 pairs of lace bug did not differ significantly from control plants. The percentage of the total leaf area affected by lace bug feeding was positively correlated with the density of lace bugs inoculated. This was also evident by the reduced chlorophyll content of leaves exposed to 9 and 15 pairs of lace bugs and their offspring. Our tests showed that one generation of feeding by the lace bug caused significant defoliation as well as reduced photosynthetic activity of remaining leaves. Continuous long term feeding by the lace bug or other potential defoliating insects could result in suppression of Chinese privet populations and possibly reduction to desirable levels.     

Potential population growth and harmful effects on humans from bed bug populations exposed to different feeding regimes

Effects of host availability and feeding period on bed bugs, Cimex lectularius (L.) (Hemiptera: Cimicidae), were measured. Population growth and the potential harmful effect of bed bug populations on human hosts were modelled. Bloodmeal sizes were affected by both feeding length and frequency, with >2‐fold difference between insects fed daily or weekly. Blood consumption increased >2‐fold between bed bugs fed occasionally and often, and 1.5‐fold between occasional and daily feeding. Bed bugs fed more often than once a week, potentially every 2–4 days. Egg production was associated with nutrition, being strongly correlated with blood consumption in the previous week. Bed bug populations can grow under different feeding regimes and are hard to control with <80% mortality. Bed bugs can survive and grow even in locations with a limited blood supply, where bed bug persistence may be important for the continual spread of populations. Persistence in non‐traditional locations and a potential association with human pathogens increase the health risks of bed bugs. Potential blood loss as a result of a bed bug can have serious consequences because uncontrolled populations can reach harmful levels in 3–8 months. The reproduction potential of bed bug populations suggests serious consequences to human health and the need for efficacious control measures.     

Dung feeding in adult scarabaeines (tunnellers and endocoprids): even large dung beetles eat small particles

Abstract 1. The maximum size of ingested particles was determined in 15 species of adult dung beetle (Scarabaeidae: Scarabaeinae) by mixing small latex or glass balls of known diameter into the dung used as food. Twelve species (tribes Coprini, Onitini, Oniticellini, and Onthophagini) were tunnellers (making dung stores for feeding and breeding in the soil below the pat) and three species (tribe Oniticellini) were endocoprids (feeding and breeding in the dung pat itself).
2. The test species, covering a wide range of body size (fresh weights 0.05–7.4 g), ingested minute particles only (maximum diameter 8–50 µm), and there was a statistically significant but numerically small increase in particle size with body weight.
3. When the effect of body size was taken into account, taxon (tribe), ecological group (tunneller/endocoprid), and dung preference (coarse/fine) had no significant effect on the size of ingested particles.
4. Tests using two tunnelling species did not indicate that beetles use their mandibles to grind dung particles prior to ingestion.
5. The results suggest essentially the same feeding mechanism in all adult tunnelling or endocoprid scarabaeines that eat fresh dung. Larger, indigestible plant fragments are avoided by filtration, and ingestion is confined to very small particles of higher nutritional value.     

Seed-carrying behavior of a stink bug, Adrisa magna Ueler (Hemiptera: Cydnidae)

We observed the seed-carrying and feeding behavior of a stink bug, Adrisa magna, under manipulated conditions in the laboratory. The bugs were most active at night but also fed on seeds under shelter during the daytime. When adults were housed on their own, they often left these shelters with the seeds in them and reused the abandoned seeds later. When several adults were introduced into a container, we found adults feeding communally and sucking on seeds abandoned by other bugs. It is suggested that seed-carrying behavior allows for communal feeding between conspecific bugs in heterogeneous environments. Received: April 19, 1999 / Accepted: May 22, 2000     

Feeding preference and movement of Nezara viridula and Euschistus serous (Hemiptera: Pentatomidae) on individual cotton plants

Experiments were conducted in an environmental growth chamber to determine the movement and feeding preferences of Nezara viridula (L.) and Euschistus serous (Say) on individual cotton plants. Fifth instars were caged by species on a single cotton plant (FM 9063 B2F) containing four discrete boll sizes ranging from 1.1 to 3.0 cm in diameter over a period of 5 d per replication. Two digital video cameras were simultaneously focused on each of the four bolls per plant to visually confirm stink bug resting and movement. During the study, a total of 4,080 h of video footage was recorded and analyzed. Results showed that N. viridula and E. serous did not prefer the exact same boll sizes. In a trial with eight stink bugs per plant, N. viridula spent more time on the three larger boll classes, 1.6-2.0, 2.1-2.5, and 2.6-3.0 cm. In a separate trial with one stink bug per plant, N. viridula spent more time on the larger boll classes while E. serous exhibited the strongest preference for 1.1-1.5 and 2.1-2.5 cm bolls. N. viridula moved more often than E. serous and both species moved more often during photophase compared with scotophase. Regardless of species or number of bugs released, bolls in the smallest boll size class fell off the plant about 3 d after the bugs were released. These results confirm that scouts who are estimating stink bug damage should select bolls in the 2.1-2.5 cm diameter boll size class.     

Estimating the mean abundance and feeding rate of a temporal ectoparasite in the wild: Afrocimex constrictus (Heteroptera: Cimicidae)

The feeding frequency of blood-feeding invertebrates in the wild is largely unknown but is an important predictor for the potential of disease transmission and for estimating the effects blood feeding may have on the host population. We present a method to estimate the mean feeding frequency per individual parasite from the frequency distribution of fed and unfed individuals in the wild. We used three populations of the cimicid species, Afrocimex constrictus, that parasitises the fruit bat Rousettus aegyptiacus. We found that the area occupied by a bug refugium was a good predictor of the number of bugs in that refugia. The estimated parasite population sizes ranged from ca. 25,000 to 3 million bugs. Their mean abundance was 1-15 bugs per host individual. Preventing feeding by bugs in their natural habitat showed that bugs took approximately 20 days to return to an unfed stage. A formula is presented by which the distribution of digestion stages in the samples was used to calculate that A. constrictus feeds approximately every 7-10 days. The dry weight of a full blood meal was approximated as 13.3 mg. Therefore A. constrictus is estimated to draw an average of 1-28 microL blood per host per day. We suggest that any of our methods can be adjusted to be used in other haematophagous insects to estimate host and parasite population size, mean parasite abundance and blood meal size as well as mean feeding frequency in the wild, including the bed bug species that parasitise humans.     

Ability of bed bug-detecting canines to locate live bed bugs and viable bed bug eggs

The bed bug, Cimex lectularius L., like other bed bug species, is difficult to visually locate because it is cryptic. Detector dogs are useful for locating bed bugs because they use olfaction rather than vision. Dogs were trained to detect the bed bug (as few as one adult male or female) and viable bed bug eggs (five, collected 5-6 d after feeding) by using a modified food and verbal reward system. Their efficacy was tested with bed bugs and viable bed bug eggs placed in vented polyvinyl chloride containers. Dogs were able to discriminate bed bugs from Camponotus floridanus Buckley, Blattella germanica (L.), and Reticulitermes flavipes (Kollar), with a 97.5% positive indication rate (correct indication of bed bugs when present) and 0% false positives (incorrect indication of bed bugs when not present). Dogs also were able to discriminate live bed bugs and viable bed bug eggs from dead bed bugs, cast skins, and feces, with a 95% positive indication rate and a 3% false positive rate on bed bug feces. In a controlled experiment in hotel rooms, dogs were 98% accurate in locating live bed bugs. A pseudoscent prepared from pentane extraction of bed bugs was recognized by trained dogs as bed bug scent (100% indication). The pseudoscent could be used to facilitate detector dog training and quality assurance programs. If trained properly, dogs can be used effectively to locate live bed bugs and viable bed bug eggs.     

Competition between the nymphs of two regionally co-occurring species of Notonecta (Hemiptera: Notonectidae)

1. Two species of freshwater invertebrate predator, Notonecta maculata and N. obliqua , showed a negative association in a series of small, man-made ponds in the Peak National Park, Derbyshire, U.K. The present study examines the potential role of interspecific interactions among nymphs on this regional distribution pattern.
2. The survival, development and feeding efficiency of nymphs were examined in laboratory and field mesocosm experiments with intra- and interspecific competition and contrasting environmental complexity.
3. Survival to adulthood and mean lifespan varied significantly in interspecific competition treatments in both laboratory and field experiments, with N . maculata showing higher survival in the simple environment and N. obliqua higher survival in the complex environment.
4. Variations in feeding efficiency were consistent with the survival trends: N. maculata had a higher efficiency in the simple environment, whereas N. obliqua had greater efficiency in the complex environment. There was evidence of a developmental response in feeding efficiency, with differences between species increasing with age.
5. These results suggest that the relative competitive abilities of the two species are affected by habitat complexity, and that competition between species may modify the species distribution where they co-occur.     

Nutritional composition of the diet of the northern yellow-cheeked crested gibbon (<Emphasis Type="Italic">Nomascus annamensis</Emphasis>) in northeastern Cambodia

This study measured the nutritional composition of foods consumed by the northern yellow-cheeked crested gibbon (Nomascus annamensis) in northeastern Cambodia. One group of N. annamensis was studied, and focal animal sampling was used to observe their feeding behavior. The study was conducted for 4 months (January–April 2015) in the dry season and 69 foods were collected for nutritional analyses. N. annamensis fed on 37 plant species, but only seven species made up more than 80% of feeding time. N. annamensis spent the majority of their time feeding on fruit (60.36%), followed by young leaves (22.60%), flowers (13.74%), and mature leaves (3.30%). Fruit had the highest concentrations of total non-structural carbohydrates, while young leaves had the highest concentration of crude protein compared to other food items. All food items had similar concentrations of lipids, except young leaves, which had lower levels than fruit and flowers. All plant parts consumed by N. annamensis had similar amounts of neutral detergent fiber, acid detergent fiber, and acid detergent lignin. Thirty-two percent of all food items contained condensed tannins, and they had a similar level of it. All foods also had similar energy contents. This study adds to our knowledge of the feeding ecology of N. annamensis by providing baseline data on the make-up of the foods they eat and may contribute to captive feeding programs, ultimately assisting the conservation of this species.     

Electropenetrography (EPG): a Breakthrough Tool Unveiling Stink Bug (Pentatomidae) Feeding on Plants

In this article, we review and discuss the potential use of EPG (electropenetrography) as a powerful tool to unveil the feeding process of phytophagous stink bugs (pentatomids). These bugs are relatively big and vigorous, which presents a problem during wiring (i.e., attachment of the gold wire on the bug’s pronotum) for use in EPG. Once this challenge was overcome, using the sand paper-and-wire technique, several species have been studied using EPG, yielding waveforms that, coupled with histological studies, revealed the ingestion sites on different host plants. These sites include vascular tissues (xylem and phloem), parenchyma tissue, and seed endosperm. Stink bugs usually feed by secreting a gelling saliva to create a salivary sheath that surrounds the stylets and anchors/supports/lubricates them. However, using the cell rupture feeding strategy and the tactic of combined laceration (mechanical movements of the stylets) and maceration (action of chemical enzymes) breaks the plant cells enabling ingestion. The number of ingestion events and their duration is variable according to the feeding site. Waveforms generated have typical patterns according to the feeding site. Recent studies with several species of stink bugs have started to demonstrate the potential of EPG as a tool to unveil their feeding behavior. This may also be useful in the applied field of stink bug management, such as the development and screening of resistant genotypes and the action of chemical insecticides affecting their feeding and survivorship.     

Old World phytophagous bats (Megachiroptera) and their food plants: a survey

The Megachiroptera, with a single family, 43 genera and 174 species, are confined to the Old World tropics and subtropics where they are exclusively phytophagous, feeding upon floral resources (largely nectar and pollen but also petals and bracts), fruit, leaves and water. A literature survey revealed information on the food sources for only 21 genera of bats, these feeding upon 75 genera of flowers, 145 genera of fruit and 10 genera of leaves in a total of 34 orders, 66 families and 198 genera. The bats are likely to pollinate flowers of at least 31 genera and presumably may disperse the seeds of the majority of fruits consumed. Despite our ignorance about the relationship between Megachiroptera and plants, it appears that most bats are catholic in their choice of food, and most plants are visited by a diversity of bats.     

Candidates for biocontrol of Macfadyena unguis-cati in South Africa: biology, host ranges and potential impact of Carvalhotingis visenda and Carvalhotingis hollandi under quarantine conditions

Macfadyena unguis-cati (L.) Gentry (Bignoniaceae) was introduced as an ornamental in South Africa, but is fast becoming an important invasive plant in many areas. It is difficult to control the plant chemically and mechanically. The first biocontrol agent, the chrysomelid Charidotis auroguttata (Boheman), has been released. It established at some release sites, but numbers have so far remained low. Additional biocontrol agents were sought to augment C. auroguttata. The potential host ranges of two foliage feeding lace bugs, Carvalhotingis visenda (Drake and Hambleton) and C. hollandi (Drake) (Hemiptera: Tingidae) were evaluated on the basis of nymphal no-choice and adult multi-choice tests involving 23 plant species in 11 families. In no-choice tests, nymphs of both species were able to survive and complete development on M. unguis-cati only, and adults of both species only fed and oviposited on M. unguis-cati during the adult multi-choice tests. Host specificity tests thus confirm that the tingids are highly host specific biocontrol agents, and will not pose risk to any non-target plants in South Africa. A study to determine the potential impact of C. hollandi nymphal feeding on M. unguis-cati showed a significant decrease in the chlorophyll contents of leaves when compared to those of control plants. These studies indicate that, once released, the two lace bug species could contribute significantly to the biological control of M. unguis-cati in South Africa.