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1.
Abstract: Life history parameters in two generations of endoparasitoid Campoletis chlorideae (Uchida) were examined using Bacillus thuringiensis (Bt)-resistant Helicoverpa armigera (Hübner) larvae feeding on B. thuringiensis toxin Cry1Ac. In the laboratory, Bt toxin was fed to Bt-resistant host larvae continuously in case of Bt treatment and only before or after the host larvae were parasitized in Bt–P and P–Bt treatments, respectively. C. chlorideae pupae developed faster in Bt treatment than non-Bt treatment. The shortened pupal stage duration was mainly because of the feeding of host larvae on Bt-diet before being parasitized. Body length of adult male C. chlorideae developed inside Bt-treated Bt-resistant (Bt–Bt) H. armigera larvae significantly decreased, especially in host larvae feeding on Bt-diet after being parasitized. However, survival, pupal mortality and adult longevity of C. chlorideae were almost unaffected in Bt-resistant H. armigera larvae feeding on Bt-toxin. Furthermore, Bt-treated host larvae had the same effect on the F1 progeny of C. chlorideae as the previous generation, and there was no significant difference between generations. This experiment suggests that there is very limited effect on the life history parameters in two generations of C. chlorideae parasitizing Bt–Bt H. armigera larvae. But both generations of C. chlorideae are affected when Bt-resistant H. armigera larvae fed on Bt toxin for different durations. 相似文献
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转基因作物对生物多样性的影响 总被引:45,自引:3,他引:42
转基因作物对生物多样性的影响是重要的环境问题之一,近年来,已有这类实验的报道。夏敬源等的研究说明大田种植转基因Bt虫棉花对棉铃虫优势寄生性天敌齿唇姬蜂(Campoletis chlorideae)和侧沟绿茧蜂(Microplitis sp.)的寄生率、羽化率和蜂茧质量造成严惩的危害。国际上一些昆虫学家也做了很好的工作,尤其1999年5月Losey在Nature上发表转基因Bt抗虫玉米在实验室水平上引起大斑蝶死亡报道后,世界范围内引起极大的反响,在有关经遗传修饰生物体(GMOs)的潜在生态风险和人体健康影响的问题上美国始络抱抵制的强硬态度,这次也开始有所所松动。两个有影响的美国玉米加工公司富布不再接受欧盟拒绝进口的转基因玉米品种。欧盟宣布在进一步研究得到结论前冻结转基因作物产品进口的审批。欧盟所属有关国家有的禁止进口,有的反对欧盟审批。科技界及公众众说纷纭。建议我国政府要加强GMOs潜在生态风险及对公众健康的研究及商业化生产后的监测。 相似文献
3.
Effects of transgenic Cry1A+CpTI cotton and Cry1Ac toxin on the parasitoid, Campoketis chlorideae (Hymenoptera:Ichneumonidae) 总被引:2,自引:0,他引:2
Abstract The effect of transgenic double genes, Cry1A + CpTI cotton and Cry1Ac toxin on the parasitoid, Campoketis chlorideae Uchida of cotton bollworm, Helicoverpa armigera (Hübner), was investigated in the laboratory. Helicoverpa armigera larvae when in the first, second and third instar could not survive if fed on transgenic cotton leaves. Consequently, C. chlorideae larvae could not complete their development if parasitizing on such hosts. After H. armigera larvae were reared on transgenic or traditional cotton leaves for 12J48 hours, they were parasitized by C. chlorideae females. Parasitized larvae continued to feed on transgenic or traditional cotton for 12–48 h. The present results showed that the body weight of larvae of the parasitoids were significantly reduced when parasitized hosts fed on transgenic cotton leaves compared to those fed on traditional cotton. Duration of egg and larvae stage were significantly prolonged, pupal and adult weight of C. chloridae was decreased when the host larvae fed on transgenic cotton leaves longer than 48 h. The development duration of C. chlorideae pupae on the hosts fed on transgenic cotton leaves in each treatment was not significantly different from those of controls. The longevity of parasitoid females and males fed with a solution containing Cry1Ac toxin was not significantly different with that of the control. 相似文献
4.
The ichneumonid parasitoid, C. chlorideae is an important natural enemy of pod borer/bollworm, Helicoverpa armigera (Hubner) in different agro-ecosystems. The sex-ratio of parasitoids has an important bearing on the population build up of the natural enemies for biological control of insect pests. Therefore, the present studies were conducted to gain an understanding of the influence of mating behaviour and abundance of the insect host on fecundity and sex-ratio of the parasitoid, C. chlorideae. There was no significant influence of number of matings and abundance of the insect host on cocoon formation, adult emergence, and larval and pupal periods of C. chlorideae. However, fecundity and female longevity were significantly influenced by mating and abundance of the insect host. There was a significant and positive correlation (r = 0.84**) between longevity and fecundity of C. chlorideae females. The unmated C. chlorideae females produced only males. Nearly 20% of the females that had mated twice were able to parasitize the H. armigera larvae successfully. The sex-ratio of the progeny from females that had mated twice was male biased. Females mated with males from the unmated females produced significantly less numbers of females than those mated with males from the fertilized females, indicating genetic regulation of sex-ratio in C. chlorideae. 相似文献
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Complexity in specificities and expression of Helicoverpa armigera gut proteinases explains polyphagous nature of the insect pest 总被引:1,自引:0,他引:1
Patankar AG Giri AP Harsulkar AM Sainani MN Deshpande VV Ranjekar PK Gupta VS 《Insect biochemistry and molecular biology》2001,31(4-5):453-464
Helicoverpa armigera is a devastating pest of cotton and other important crop plants all over the world. A detailed biochemical investigation of H. armigera gut proteinases is essential for planning effective proteinase inhibitor (PI)-based strategies to counter the insect infestation. In this study, we report the complexity of gut proteinase composition of H. armigera fed on four different host plants, viz. chickpea, pigeonpea, cotton and okra, and during larval development. H. armigera fed on chickpea showed more than 2.5- to 3-fold proteinase activity than those fed on the other host plants. H. armigera gut proteinase composition revealed the predominance of serine proteinase activity; however, the larvae fed on pigeonpea revealed the presence of metalloproteases and low levels of aspartic and cysteine proteases as well. Gut proteinase activity increased during larval development with the highest activity seen in the fifth instar larvae which, however, declined sharply in the sixth instar. Over 90% of the gut proteinase activity of the fifth instar larvae was of the serine proteinase type, however, the second instar larvae showed the presence of proteinases of other mechanistic classes like metalloproteases, aspartic and cysteine proteases along with serine proteinase activity as evident by inhibition studies. Analysis of fecal matter of larvae showed significant increase in proteinase activity when fed on an artificial diet with or without non-host PIs than larvae fed on a natural diet. The diversity in the proteinase activity observed in H. armigera gut and the flexibility in their expression during developmental stages and depending upon the diet provides a base for selection of proper PIs for insect resistance in transgenic crop plants. 相似文献
7.
Sharma HC Pampapathy G Dhillon MK Ridsdill-Smith JT 《Journal of economic entomology》2005,98(2):568-576
The noctuid Helicoverpa armigera (Hübner) is a major insect pest of chickpea Cicer arietinum L., pigeonpea Cajanus cajan (L.) Millsp., peanut Arachis hypogaea L., and cotton Gossypium spp., and host plant resistance is an important component for managing this pest in different crops. Because of variations in insect density and staggered flowering of the test material, it is difficult to identify cultivars with stable resistance to H. armigera across seasons and locations. To overcome these problems, we standardized the detached leaf assay to screen for resistance to this pest in chickpea, pigeonpea, peanut, and cotton under uniform insect pressure under laboratory conditions. Terminal branch (three to four fully expanded leaves) of chickpea, first fully expanded leaf of cotton, trifoliate of pigeonpea, or quadrifoliate of peanut, embedded in 3% agar-agar in a plastic cup/jar of appropriate size (250-500-ml capacity) infested with 10-20 neonate larvae can be used to screen for resistance to H. armigera. This technique keeps the leaves in a turgid condition for approximately 1 wk. The experiments can be terminated when the larvae have caused > 80% leaf damage in the susceptible check or when differences in leaf feeding between the resistant and susceptible checks are maximum. Detached leaf assay can be used as a rapid screening technique to evaluate germplasm, segregating breeding materials, and mapping populations for resistance to H. armigera in a short span of time with minimal cost, and under uniform insect infestation. It also provides useful information on antibiosis component of resistance to the target insect pest. 相似文献
8.
转CpTI-Bt基因棉和转Bt基因棉对棉铃虫幼虫存活、生长及营养利用的影响 总被引:6,自引:0,他引:6
2000年7月中旬和8月中旬, 分别测定了采自田间的转CpTI-Bt基因双价抗虫棉(SGK321, 以下简称CpTI-Bt棉)和转Bt基因抗虫棉(中30,以下简称Bt棉)对棉铃虫Helicoverpa armigera幼虫存活、生长的影响。结果表明:7月中旬两种转基因抗虫棉抗虫效果均较好,尤其是CpTI-Bt棉棉叶和花瓣对4龄幼虫3天内致死率为92%以上;8月中旬两种转基因棉的抗虫活性均明显降低,且Bt棉的杀虫活性显著低于CpTI-Bt棉,其幼虫死亡率与对照受体棉中16的死亡率之间无显著差异,仅显著抑制了幼虫的生长;石远321(SGK321受体品系)的花瓣具有一定的抗虫活性,可显著降低取食幼虫的体重,甚至造成部分幼虫死亡; CpTI-Bt棉中,花瓣和棉叶的抗虫性明显高于蕾和铃心。对5龄幼虫取食棉铃1日后的营养指标测定结果显示: 两种转基因抗虫棉处理的幼虫相对生长率和相对取食量均显著低于石远321,但两者之间无显著差异; CpTI-Bt棉处理的幼虫近似消化率显著低于石远321和Bt棉,但其食物利用率显著高于石远321和Bt棉。 相似文献
9.
Lawo NC Mahon RJ Milner RJ Sarmah BK Higgins TJ Romeis J 《Applied and environmental microbiology》2008,74(14):4381-4389
The use of genetically modified (Bt) crops expressing lepidopteran-specific Cry proteins derived from the soil bacterium Bacillus thuringiensis is an effective method to control the polyphagous pest Helicoverpa armigera. As H. armigera potentially develops resistance to Cry proteins, Bt crops should be regarded as one tool in integrated pest management. Therefore, they should be compatible with biological control. Bioassays were conducted to understand the interactions between a Cry2Aa-expressing chickpea line, either a susceptible or a Cry2A-resistant H. armigera strain, and the entomopathogenic fungus Metarhizium anisopliae. In a first concentration-response assay, Cry2A-resistant larvae were more tolerant of M. anisopliae than susceptible larvae, while in a second bioassay, the fungus caused similar mortalities in the two strains fed control chickpea leaves. Thus, resistance to Cry2A did not cause any fitness costs that became visible as increased susceptibility to the fungus. On Bt chickpea leaves, susceptible H. armigera larvae were more sensitive to M. anisopliae than on control leaves. It appeared that sublethal damage induced by the B. thuringiensis toxin enhanced the effectiveness of M. anisopliae. For Cry2A-resistant larvae, the mortalities caused by the fungus were similar when they were fed either food source. To examine which strain would be more likely to be exposed to the fungus, their movements on control and Bt chickpea plants were compared. Movement did not appear to differ among larvae on Bt or conventional chickpeas, as indicated by the number of leaflets damaged per leaf. The findings suggest that Bt chickpeas and M. anisopliae are compatible to control H. armigera. 相似文献
10.
【目的】针对由Bt棉花和玉米构成的华北农田景观, 探讨不同寄主作物对棉铃虫 Helicoverpa armigera 越冬抗寒的影响。【方法】2013年在河北廊坊科研中试基地的作物田中将棉铃虫幼虫接到Bt棉和玉米上, 比较取食不同作物后棉铃虫的化蛹率、存活率和越冬蛹羽化率;在室内控制条件下分别用棉蕾和鲜玉米粒饲喂棉铃虫幼虫。 测定滞育蛹和非滞育蛹的鲜重、干重、以及脂肪、糖原和低分子物质含量等指标, 比较取食不同作物后棉铃虫的抗寒能力。【结果】取食玉米的棉铃虫滞育蛹干重(117.5 mg)、脂肪含量(457.2 μg/mg DW)以及海藻糖浓度(86.45 μg/g)均显著高于取食Bt棉的棉铃虫滞育蛹干重(56.6 mg)、脂肪含量(239.6 μg/mg DW)以及海藻糖浓度(13.87 μg/g);取食玉米的棉铃虫冰点(-10.2℃)显著低于取食Bt棉的棉铃虫冰点(-6.5℃)。【结论】结果表明取食玉米更加有利于棉铃虫越冬。 据历史数据, 近年来玉米种植面积不断增加, 这将提高棉铃虫成功越冬比率, 对棉铃虫种群扩张起到促进作用, 因此注重玉米上棉铃虫的防治尤为重要。 相似文献
11.
Helicoverpa armigera, a polyphagous lepidopteron insect pest causes severe yield loss in cotton, legumes, tomato, okra and other crops. Application of chemical pesticides although effective, has human health and environmental safety concerns. Moreover, development of resistance against most of the available pesticides is compelling to look for alternative strategies. Adoption of Bt transgenic crops have resulted in reduction in pesticide consumption and increasing crop productivity. However, sustainability of Bt transgenic crops is threatened by the emergence of insect resistance. In the present study potential insecticidal siRNA were identified in six H. armigera horrhonal pathway genes. Out of over 2000 computationally identified siRNA, 16 most promising siRNA were selected that address the biosafety concerns and have high potential of targeted gene silencing. These siRNA will be useful for chemical synthesis, in insect feeding assays and knockdown the target H. armigera hormone biosynthesis, consequently obstructing the completion of insect life cycle. The siRNA have a great potential of deployment to control H. annrmigera alone as well as with Bt for insect resistance management. 相似文献
12.
Abstract: Propylaea japonica is an important predatory insect of common cotton pests. To assess the ecological effects of transgenic Bt cotton, expressing Cry1Ac toxin, on this predator, we examined the life history parameters of P. japonica for two generations by feeding them with Bt-resistant Helicoverpa armigera . After ingesting Bt-treated Bt-resistant H. armigera larvae in the third and fourth instar, the body mass and body length of adult P. japonica decreased, a combined effect of poor prey quality and Cry1Ac Bt-toxin may account for these effects. However, larval survivorship and development in these two instars, pupal mortality, fecundity and adult longevity of P. japonica were not affected in both the generations. These results suggest that ingesting Bt-toxin Cry1Ac-treated pests in advanced larval stage might have no significant effect on the fitness of predator P. japonica . 相似文献
13.
转Bt棉花对蜘蛛生长发育及捕食行为的影响 总被引:4,自引:0,他引:4
室内评价了取食转Bt棉叶的棉铃虫幼虫对草间钻头蛛和八斑鞘腹蛛生长发育的影响.并通过捕食功能反应评价了取食了Bt棉叶的棉铃虫幼虫对成熟草间钻头蛛捕食行为的影响.室内饲养实验结果表明草间钻头蛛、八斑鞘腹蛛取食用转Bt棉叶处理的棉铃虫幼虫与取食普通棉叶处理的棉铃虫幼虫的发育历期、成蛛体重都没有显著差异.捕食功能反应实验结果表明草间钻头蛛对棉铃虫幼虫的捕食功能反应符合HollingⅡ型圆盘方程,两组不同猎物饲养成熟的草间钻头蛛对同种处理的棉铃虫幼虫的捕食行为没有显著差异.综合考虑:转Bt棉对蜘蛛生长发育、捕食能力没有显著的负作用. 相似文献
14.
Improvement of pest resistance in transgenic tobacco plants expressing dsRNA of an insect-associated gene EcR 总被引:2,自引:0,他引:2
The adoption of pest-resistant transgenic plants to reduce yield loss and pesticide utilization has been successful in the past three decades. Recently, transgenic plant expressing double-stranded RNA (dsRNA) targeting pest genes emerges as a promising strategy for improving pest resistance in crops. The steroid hormone, 20-hydroxyecdysone (20E), predominately controls insect molting via its nuclear receptor complex, EcR-USP. Here we report that pest resistance is improved in transgenic tobacco plants expressing dsRNA of EcR from the cotton bollworm, Helicoverpa armigera, a serious lepidopteran pest for a variety of crops. When H. armigera larvae were fed with the whole transgenic tobacco plants expressing EcR dsRNA, resistance to H. armigera was significantly improved in transgenic plants. Meanwhile, when H. armigera larvae were fed with leaves of transgenic tobacco plants expressing EcR dsRNA, its EcR mRNA level was dramatically decreased causing molting defects and larval lethality. In addition, the transgenic tobacco plants expressing H. armigera EcR dsRNA were also resistant to another lepidopteran pest, the beet armyworm, Spodoptera exigua, due to the high similarity in the nucleotide sequences of their EcR genes. This study provides additional evidence that transgenic plant expressing dsRNA targeting insect-associated genes is able to improve pest resistance. 相似文献
15.
Vijaykumar Bashasab F Krishnareddy KB Kuruvinashetti MS Patil BV 《Journal of economic entomology》2007,100(3):903-908
Helicoverpa armigera (Hiibner) (Lepidoptera: Noctuidae) is a well-known polyphagous insect pest. Mating compatibility among the insects occurring on different host plants is essential for free gene flow among populations. We tested the extent of crossability and fecundity of the insects that survived on Bacillus thuringiensis (Bt) cotton with those occurring on pigeon pea, Cajanus cajun (L.) Millsp., non-Bt cotton, Gossypium hirsutum L.; sunflower, Helianthus annuus; sorghum, Sorghum bicolor L. Moench.; okra, Abelmoschus moschatus Medikus; chickpea, Cicer arietinum L.; marigold, Tagetes spp.; and tomato, Lycopersicum esculentum L., crops. The insects from different crops were freely crossable with those collected from Bt cotton and among themselves. The average fecundity across different crosses ranged from 314.1 to 426.3 in direct and from 305.8 to 421.7 eggs per female in reciprocal crosses. In any given cross, a minimum of 85.89% egg hatch was recorded. Furthermore, the F1 individuals of different cross combinations were found to cross freely with their parents (BC1) and among themselves with similar fecundity and egg hatch. High crossability among H. armigera occurring on different host plants suggests that crop mosaics that may exist in countries such as India could play an important role as natural, nonstructured refugia and prolong the durability of the genes deployed for controlling this insect. 相似文献
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棉酚对棉铃虫生长及其寄生物齿唇姬蜂发育的影响(英文) 总被引:1,自引:0,他引:1
以中国北方棉区的主要害虫棉铃虫Helicoverpaarmigera(Hbner)及其内寄生蜂棉铃虫齿唇姬蜂Campoletischlorideae(Uchida)为试虫,研究了棉花重要抗虫次生性物质棉酚对棉铃虫生长和齿唇姬蜂发育的影响。0.1%的棉酚对棉铃虫幼虫有生长刺激作用,0.5%的棉酚则对棉铃虫幼虫生长有抑制作用。齿唇姬蜂成虫在寄生时对寄主大小有较严格的选择性,92%的被寄生幼虫体重小于18mg。0.1%的棉酚使寄主适合寄生期缩短10.75%;0.5%棉酚使寄主适合寄生期延长28.15%。棉酚对齿唇姬蜂发育有负效应。0.1%棉酚对寄主的生长刺激作用,不能使蜂成虫体重显著增加,但可显著延长蜂卵和幼虫期,缩短蛹期;高浓度的生长抑制作用,导致蜂成虫体重显著减轻,蛹期缩短,蜂卵和幼虫期亦显著延长。据此,对棉花抗虫性与生物防治在害虫综合防治实践中应用的协调性进行了讨论。 相似文献
18.
Host crop influence on the susceptibility of the American bollworm, Helicoverpa armigera, to Bacillus thuringiensis ssp. kurstaki HD-73 总被引:2,自引:0,他引:2
G.T. Gujar A. Mittal Archana Kumari & V. Kalia 《Entomologia Experimentalis et Applicata》2004,113(3):165-172
Studies on the susceptibility of F1 neonates of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) collected from chickpea in Delhi and cotton in Punjab, Haryana and Rajasthan in northern India, to Bacillus thuringiensis ssp. kurstaki HD‐73, and the impact of host crop diets on insect susceptibility, were carried out by diet incorporation bioassays. The susceptibility of F1 neonates of H. armigera to Bacillus thuringiensis ssp. kurstaki HD‐73 ranged from twofold (LC50 96 h, 84.5–164.2 µg (ai) l?1) for chickpea to about fivefold (LC50 96 h, 51.1–247.7 µg (ai) l?1) for cotton. The F1 neonates of insects collected from pearl millet were twice as tolerant as those collected from cotton and sunflower at Sirsa to B. thuringiensis ssp. kurstaki HD‐73, suggesting that there was an influence of host crops on insect susceptibility. Insects originally collected from cotton fields at Bhatinda and reared for four generations on a chickpea‐based meridic diet were used to initiate host‐specific colonies of H. armigera. These host‐specific colonies were allowed to complete one generation on meridic diets prepared with different hosts, viz., cabbage, cauliflower, chickpea, green pea, pearl millet, and pigeon pea. Larvae of H. armigera were heaviest on the 15th day, and had a higher growth rate on a pigeon pea‐based diet than all other host diets. The larval period was shorter on chickpea and pigeon pea, with higher percentage pupation than all other host‐diets. The pupal weight of H. armigera was greater on chickpea and pigeon pea diets than on other host diets. The growth and development of larvae was significantly poorer on pearl millet diet than on other host diets. The F1 neonates of H. armigera belonging to cabbage, cauliflower, and pearl millet host‐specific colonies were more susceptible than those belonging to chickpea, green pea, and pigeon pea host‐specific colonies to B. thuringiensis ssp. kurstaki HD‐73, suggesting the importance of proteinaceous nutrients in tolerance. The F1 neonates of the pearl millet colony of H. armigera grown on a chickpea‐diet for 4 days were significantly more tolerant to B. thuringiensis ssp. kurstaki HD‐73 than those reared on the pearl millet‐based diet. These studies show the impact of the host diet of H. armigera on tolerance to B. thuringiensis. 相似文献
19.
The density of Helicoverpa armigera (Hübner) populations on Bacillus thuringiensis Berliner (Bt) transgenic cotton, corn, peanut, and soybean; differences in its development on Bt cotton and common (nontransgenic) cotton; and the potential for mating among populations from Bt cotton fields and other crop fields were investigated in the suburbs of Xinxiang City (Henan Province) and Langfang City (Hebei Province) in the southern and northern parts of north China, respectively. Although development of H. armigera on Bt cotton was much slower than on common cotton, there was a still high probability of mating between populations from Bt cotton and other sources due to the scattered emergence pattern of H. armigera adults, and overlap of the second and third generations. In a cotton and corn growing region, early and late planted corn provided suitable refugia for the third and fourth generations of H. armigera, but not for the second generation. In a cotton and soybean/ peanut mix system, noncotton crops provided a natural refugia from the second- to fourth-generation H. armigera, but function of the refuge would closely depend on the proportion of Bt cotton. Consequently, it may be necessary to compensate the original mixed cropping patterns in different areas for delaying resistance development of H. armigera to Bt cotton. 相似文献
20.
Abstract Helicoverpa armigera oviposition preference for, and larval development on sorghum hybrids with differing resistance to sorghum midge, Stenodiplosis sorghicola , were investigated. When H. armigera larvae were fed seed of resistant and susceptible hybrids in the laboratory there were no differences in larval and pupal sizes or the rate of development. The same result was recorded when larvae fed on panicles on plants in a glasshouse. On some sampling occasions, significantly more eggs were laid on panicles of resistant hybrids in the field. This occurred when plants were in plots and also in a mixed planting. Midge-resistance status did not affect levels of egg parasitism. In a field study using recombinant inbred lines between a midge-resistant and a midge-susceptible line, no relationship was found between level of resistance and oviposition of H. armigera . We conclude that, although midge-resistant hybrids are sometimes preferred for oviposition by H. armigera, the resistance per se does not determine this preference. Egg survival, larval survival, development and resultant damage are not significantly affected by the midge-resistance status of the host. 相似文献