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Dr. L. Čelakovský 《Plant Systematics and Evolution》1893,43(4):143-147
Ohne ZusammenfassungDas Referat erstreckt sich auf den Zeitraum vom 1. Juli 1891 bis 10. März 1893. 相似文献
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Frant A. Novák 《Plant Systematics and Evolution》1915,65(10-12):324-324
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F. Gruber und I. Waldenburg 《Molecular & general genetics : MGG》1937,72(1):501-518
Ohne Zusammenfassung 相似文献
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Yang-Dong Guo Hisano Hisano Yoshiya Shimamoto Toshihiko Yamada 《Plant Cell, Tissue and Organ Culture》2009,96(2):219-227
Genetic transformation of androgenic-derived amphidiploid Festulolium plants (Lolium perenne L. × Festuca pratensis Huds., 2n = 4x = 28) by Agrobacterium tumefaciens has been achieved. Anther culture-induced calli of Festulolium “Bx351” were inoculated with Agrobacterium tumefaciens strain LBA4404 carrying pIG121-Hm encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes under the control of a CaMV 35S promoter. Twenty-three putative transformants were obtained from the hygromycin selection,
19 of which (82.6%) showed GUS activity. The integration of transgene was detected by using genomic DNA PCR analysis, RT-PCR
analysis and Southern blot hybridization, respectively, which revealed that foreign gene was integrated into the genomes of
dihaploid transformants (2n = 2x = 14). The haploid embryogenic system offers a stable means of transformation, as the introduced
trait can be readily fixed through chromosome doubling.
An erratum to this article can be found at 相似文献
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