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1.
Hanne Sogge Thomas Rohrlack Trine B. Rounge J?rn Henrik S?nsteb? Ave Tooming-Klunderud Tom Kristensen Kjetill S. Jakobsen 《Applied and environmental microbiology》2013,79(2):508-515
Several Planktothrix strains, each producing a distinct oligopeptide profile, have been shown to coexist within Lake Steinsfjorden (Norway). Using nonribosomal peptide synthetase (NRPS) genes as markers, it has been shown that the Planktothrix community comprises distinct genetic variants displaying differences in bloom dynamics, suggesting a Planktothrix subpopulation structure. Here, we investigate the Planktothrix variants inhabiting four lakes in southeast of Norway utilizing both NRPS and non-NRPS genes. Phylogenetic analyses showed similar topologies for both NRPS and non-NRPS genes, and the lakes appear to have similar structuring of Planktothrix genetic variants. The structure of distinct variants was also supported by very low genetic diversity within variants compared to the between-variant diversity. Incongruent topologies and split decomposition revealed recombination events between Planktothrix variants. In several strains the gene variants seem to be a result of recombination. Both NRPS and non-NRPS genes are dominated by purifying selection; however, sites subjected to positive selection were also detected. The presence of similar and well-separated Planktothrix variants with low internal genetic diversity indicates gene flow within Planktothrix populations. Further, the low genetic diversity found between lakes (similar range as within lakes) indicates gene flow also between Planktothrix populations and suggests recent, or recurrent, dispersals. Our data also indicate that recombination has resulted in new genetic variants. Stability within variants and the development of new variants are likely to be influenced by selection patterns and within-variant homologous recombination. 相似文献
2.
The evolutionary dynamics of HIV during the chronic phase of infection is driven by the host immune response and by selective pressures exerted through drug treatment. To understand and model the evolution of HIV quantitatively, the parameters governing genetic diversification and the strength of selection need to be known. While mutation rates can be measured in single replication cycles, the relevant effective recombination rate depends on the probability of coinfection of a cell with more than one virus and can only be inferred from population data. However, most population genetic estimators for recombination rates assume absence of selection and are hence of limited applicability to HIV, since positive and purifying selection are important in HIV evolution. Yet, little is known about the distribution of selection differentials between individual viruses and the impact of single polymorphisms on viral fitness. Here, we estimate the rate of recombination and the distribution of selection coefficients from time series sequence data tracking the evolution of HIV within single patients. By examining temporal changes in the genetic composition of the population, we estimate the effective recombination to be ρ = 1.4±0.6×10−5 recombinations per site and generation. Furthermore, we provide evidence that the selection coefficients of at least 15% of the observed non-synonymous polymorphisms exceed 0.8% per generation. These results provide a basis for a more detailed understanding of the evolution of HIV. A particularly interesting case is evolution in response to drug treatment, where recombination can facilitate the rapid acquisition of multiple resistance mutations. With the methods developed here, more precise and more detailed studies will be possible as soon as data with higher time resolution and greater sample sizes are available. 相似文献
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4.
The Evolution of Recombination: Removing the Limits to Natural Selection 总被引:11,自引:6,他引:11
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One of the oldest hypotheses for the advantage of recombination is that recombination allows beneficial mutations that arise in different individuals to be placed together on the same chromosome. Unless recombination occurs, one of the beneficial alleles is doomed to extinction, slowing the rate at which adaptive mutations are incorporated within a population. We model the effects of a modifier of recombination on the fixation probability of beneficial mutations when beneficial alleles are segregating at other loci. We find that modifier alleles that increase recombination do increase the fixation probability of beneficial mutants and subsequently hitchhike along as the mutants rise in frequency. The strength of selection favoring a modifier that increases recombination is proportional to λ(2)Sδr/r when linkage is tight and λ(2)S(3)δ r/N when linkage is loose, where λ is the beneficial mutation rate per genome per generation throughout a population of size N, S is the average mutant effect, r is the average recombination rate, and δr is the amount that recombination is modified. We conclude that selection for recombination will be substantial only if there is tight linkage within the genome or if many loci are subject to directional selection as during periods of rapid evolutionary change. 相似文献
5.
A. Cody Springman David W. Lacher Guangxi Wu Nicole Milton Thomas S. Whittam H. Dele Davies Shannon D. Manning 《Journal of bacteriology》2009,191(17):5419-5427
Transmission of group B Streptococcus (GBS) from mothers to neonates during childbirth is a leading cause of neonatal sepsis and meningitis. Although subtyping tools have identified specific GBS phylogenetic lineages that are important in neonatal disease, little is known about the genetic diversity of these lineages or the roles that recombination and selection play in the generation of emergent genotypes. Here, we examined genetic variation, selection, and recombination in seven multilocus sequence typing (MLST) loci from 94 invasive, colonizing, and bovine strains representing 38 GBS sequence types and performed DNA sequencing and PCR-based restriction fragment length polymorphism analysis of several putative virulence genes to identify gene content differences between genotypes. Despite the low level of diversity in the MLST loci, a neighbor net analysis revealed a variable range of genetic exchange among the seven clonal complexes (CCs) identified, suggesting that recombination is partly responsible for the diversity observed between genotypes. Recombination is also important for several virulence genes, as some gene alleles had evidence for lateral gene exchange across divergent genotypes. The CC-17 lineage, which is associated with neonatal disease, is relatively homogeneous and therefore appears to have diverged independently with an exclusive set of virulence characteristics. These data suggest that different GBS genetic backgrounds have distinct virulence gene profiles that may be important for disease pathogenesis. Such profiles could be used as markers for the rapid detection of strains with an increased propensity to cause neonatal disease and may be considered useful vaccine targets.Group B Streptococcus (GBS) is a leading cause of neonatal sepsis, pneumonia, and meningitis (51) and causes infections in pregnant women, nonpregnant adults, and the elderly with underlying medical conditions. Maternal GBS colonization is a main risk factor for neonatal disease, and roughly 20 to 40% of pregnant women are colonized (14, 23). Colonization rates of up to 31% and 34% have been documented in young men (4) and nonpregnant women (4, 42), respectively, whereas a rate of 22% has been observed in individuals over 65 years of age (18). GBS has also been identified as the cause of bovine mastitis in up to 45% of symptomatic bovines (30). Nine distinct polysaccharide capsule types (serotypes) are known, and the serotype distribution varies by population.The genetic diversity of GBS populations has been studied using a variety of different methods, including restriction fragment length polymorphism (RFLP) (24), ribotyping (5, 25), pulsed-field gel electrophoresis (49), multilocus enzyme electrophoresis (MLEE) (45), random amplification of polymorphic DNA (36), restriction digestion pattern (RDP) typing (53), and multilocus sequence typing (MLST) (28). By utilizing methods that focus on conserved genetic changes within GBS strains, virulent GBS clones that have diversified genetically can be identified. Both MLEE and MLST can distinguish the major GBS serotype III clones associated with neonatal invasive disease as sequence type 17 (ST-17) in the MLST system (28, 29, 38) or electrophoretic type 1 in the MLEE system (45). This clone is also evident in the RDP system as RDP-III (53).A recent study of 75 GBS strains representing different sources and STs reported that the ST-17 lineage is relatively homogeneous and contains a unique set of surface proteins (9). Homogeneity within a GBS lineage that is significantly associated with neonatal disease is likely important for disease pathogenesis, though few studies have been conducted to identify specific differences in virulence characteristics between lineages. Similarly, the roles of selection and recombination in the generation of STs, as well as known virulence genes, have only recently been explored and require further investigation (9a). Here, we assess the genomic diversity of GBS strains representing a variety of common clonal genotypes, examine evidence for selection and recombination, and evaluate the extent of DNA polymorphism and allelic variation in several putative virulence genes. 相似文献
6.
Genetic differentiation along environmental clines is often observed as a result of interplay between gene flow and natural
selection. In order to understand the relative roles of these processes in shaping this differentiation, we designed a study
in which we used two approaches that have not previously been combined, the Q
ST–F
ST comparison and crossbreeding. We examined (1) interpopulation phenotypic and genetic (AFLP) variation, and (2) performance
of interpopulation hybrids in a common annual Senecio glaucus. Fitness of interpopulation hybrids (F1 and F2) was tested under simulated population natural conditions in terms of aridity
and analyzed for a relationship with (1) spatial distance and (2) environmental differences (amount of annual rainfall). While
phenotypic variation corresponded to the clinal changes in aridity along population locations, viz. narrower and longer leaves,
longer leaf outgrowths and advanced flowering in more arid environments, the F
ST < 0.1 calculated from AFLP data suggested intensive interpopulation gene flow, with little if any contribution of genetic
drift. Performance of hybrids in simulated natural environments revealed heterosis in F1, but a hybrid breakdown in F2 generation.
These effects were related to both the spatial distance between hybrid parents and their population rainfall differences.
The detected clinal phenotypic variation and outbreeding depression in F2 strongly support presence of aridity-induced clinal
natural selection, which is matched by the observed Q
ST ≫ F
ST. From this we conclude that Q
ST–F
ST comparison can detect effect of diversifying selection when patterns of phenotypic variation across sampled locations can
be reliably predicted from environmental variation. 相似文献
7.
Evolution of the F-Box Gene Family in Euarchontoglires: Gene Number Variation and Selection Patterns
F-box proteins are substrate adaptors used by the SKP1–CUL1–F-box protein (SCF) complex, a type of E3 ubiquitin ligase complex in the ubiquitin proteasome system (UPS). SCF-mediated ubiquitylation regulates proteolysis of hundreds of cellular proteins involved in key signaling and disease systems. However, our knowledge of the evolution of the F-box gene family in Euarchontoglires is limited. In the present study, 559 F-box genes and nine related pseudogenes were identified in eight genomes. Lineage-specific gene gain and loss events occurred during the evolution of Euarchontoglires, resulting in varying F-box gene numbers ranging from 66 to 81 among the eight species. Both tandem duplication and retrotransposition were found to have contributed to the increase of F-box gene number, whereas mutation in the F-box domain was the main mechanism responsible for reduction in the number of F-box genes, resulting in a balance of expansion and contraction in the F-box gene family. Thus, the Euarchontoglire F-box gene family evolved under a birth-and-death model. Signatures of positive selection were detected in substrate-recognizing domains of multiple F-box proteins, and adaptive changes played a role in evolution of the Euarchontoglire F-box gene family. In addition, single nucleotide polymorphism (SNP) distributions were found to be highly non-random among different regions of F-box genes in 1092 human individuals, with domain regions having a significantly lower number of non-synonymous SNPs. 相似文献
8.
Disequilibrium, Selection, and Recombination: Limits in Two-Locus, Two-Allele Models 总被引:2,自引:0,他引:2
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Alan Hastings 《Genetics》1981,98(3):659-668
All possible combinations of equilibria and fitnesses in two-locus, two-allele, deterministic, discrete-generation selection models are enumerated. This knowledge is used to obtain limits (which can be calculated to arbitrary precision) to the relationships among disequilibrium, selection and recombination for fixed values of allele frequencies. In all cases, the inequality|rD| < s/10 holds, where r is recombination and D is disequilibrium, and all selection coefficients lie between 1 - s and 1 + s times that of the double heterozygote. Linear programming techniques are used to calculate the minimum strength of selection needed to explain several observed nonzero values of D reported in the literature. One conclusion is that the failure to observe nonzero values of D is not surprising. 相似文献
9.
The Gp-9 gene in fire ants represents an important model system for studying the evolution of social organization in insects as well as a rich source of information relevant to other major evolutionary topics. An important feature of this system is that polymorphism in social organization is completely associated with allelic variation at Gp-9, such that single-queen colonies (monogyne form) include only inhabitants bearing B-like alleles while multiple-queen colonies (polygyne form) additionally include inhabitants bearing b-like alleles. A recent study of this system by Leal and Ishida (2008) made two major claims, the validity and significance of which we examine here. After reviewing existing literature, analyzing the methods and results of Leal and Ishida (2008), and generating new data from one of their study sites, we conclude that their claim that polygyny can occur in Solenopsis invicta in the U.S.A. in the absence of expression of the b-like allele Gp-9b is unfounded. Moreover, we argue that available information on insect OBPs (the family of proteins to which GP-9 belongs), on the evolutionary/population genetics of Gp-9, and on pheromonal/behavioral control of fire ant colony queen number fails to support their view that GP-9 plays no role in the chemosensory-mediated communication that underpins regulation of social organization. Our analyses lead us to conclude that there are no new reasons to question the existing consensus view of the Gp-9 system outlined in Gotzek and Ross (2007). 相似文献
10.
《Current biology : CB》2014,24(22):R1083-R1085
11.
Reciprocal Recombination and the Evolution of the Ribosomal Gene Family of Drosophila Melanogaster
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The role of reciprocal recombination in the coevolution of the ribosomal RNA gene family on the X and Y chromosomes of Drosophila melanogaster was assessed by determining the frequency and nature of such exchange. In order to detect exchange events within the ribosomal RNA gene family, both flanking markers and restriction fragment length polymorphisms within the tandemly repeated gene family were used. The vast majority of crossovers between flanking markers were within the ribosomal RNA gene region, indicating that this region is a hotspot for heterochromatic recombination. The frequency of crossovers within the ribosomal RNA gene region was approximately 10(-4) in both X/X and X/Y individuals. In conjunction with published X chromosome-specific and Y chromosome-specific sequences and restriction patterns, the data indicate that reciprocal recombination alone cannot be responsible for the observed variation in natural populations. 相似文献
12.
生物自然居群间的基因流不但可以阻止遗传分化以维持物种的完整性,而且也能积极响应生物多样化的进程。理解与基因流相关的适应性进化及其内在机理将有助于我们更好地认识生物物种形成和多样化的原始动力以及真正原因。该文通过对植物种内和种间居群基因流动态进行讨论,阐述了近年来有关植物基因流动态的一些重要理论观念和研究进展,以期为相关领域动态及趋势研究提供参考。 相似文献
13.
This paper proposes that alleles increasing recombination rates may be selected for as a result of the perturbing effects of the spread of selectively favored alleles on neighboring loci maintained polymorphic by sleection. The recombination genes are favored since their presence increases the production of selectively advantageous types of gametes with which they tend to remain associated. Numerical examples are presented, and some consequences of this model discussed. One such consequence is the wicespread existence of polymorphism for genes affecting recombination values. 相似文献
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15.
J. A. McKenzie 《Genetics》1975,80(2):349-361
A marked genetic differentiation to the presence of alcohol in the environment has been shown to occur between inside cellar and adjacent outside sections of a vineyard population of D. melanogaster ( McKenzie and Parsons 1974). Estimates of migration during the vintage period suggest considerable movement occurs from outside into the cellar and that the most tolerant genotypes are the most successful migrants. A quantitative model of this system suggests that the selection intensity may not be a limiting factor in maintaining the differentiation. It also suggests that gene flow must be restricted between sections of the population at all but vintage periods if this differentiation is to persist. 相似文献
16.
Senanu M. Spring-Pearson Joshua K. Stone Adina Doyle Christopher J. Allender Richard T. Okinaka Mark Mayo Stacey M. Broomall Jessica M. Hill Mark A. Karavis Kyle S. Hubbard Joseph M. Insalaco Lauren A. McNew C. Nicole Rosenzweig Henry S. Gibbons Bart J. Currie David M. Wagner Paul Keim Apichai Tuanyok 《PloS one》2015,10(10)
The pangenomic diversity in Burkholderia pseudomallei is high, with approximately 5.8% of the genome consisting of genomic islands. Genomic islands are known hotspots for recombination driven primarily by site-specific recombination associated with tRNAs. However, recombination rates in other portions of the genome are also high, a feature we expected to disrupt gene order. We analyzed the pangenome of 37 isolates of B. pseudomallei and demonstrate that the pangenome is ‘open’, with approximately 136 new genes identified with each new genome sequenced, and that the global core genome consists of 4568±16 homologs. Genes associated with metabolism were statistically overrepresented in the core genome, and genes associated with mobile elements, disease, and motility were primarily associated with accessory portions of the pangenome. The frequency distribution of genes present in between 1 and 37 of the genomes analyzed matches well with a model of genome evolution in which 96% of the genome has very low recombination rates but 4% of the genome recombines readily. Using homologous genes among pairs of genomes, we found that gene order was highly conserved among strains, despite the high recombination rates previously observed. High rates of gene transfer and recombination are incompatible with retaining gene order unless these processes are either highly localized to specific sites within the genome, or are characterized by symmetrical gene gain and loss. Our results demonstrate that both processes occur: localized recombination introduces many new genes at relatively few sites, and recombination throughout the genome generates the novel multi-locus sequence types previously observed while preserving gene order. 相似文献
17.
Effects of the rad52 mutation in Saccharomyces cerevisiae on meiotic, γ-ray-induced, UV-induced and spontaneous mitotic recombination were studied. The rad52/rad52 diploids undergo premeiotic DNA synthesis; sporulation occurs but inviable spores are produced. Both intra and intergenic recombination during meiosis were examined in cells transferred from sporulation medium to vegetative medium at different time intervals. No intragenic recombination was observed at the his1–1/his1–315 and trp5–2/trp5–48 heteroalleles. Gene-centromere recombination also was not observed in rad52/rad52 diploids. No γ-ray- or UV-induced intragenic mitotic recombination is seen in rad52/rad52 diploids. The rate of spontaneous mitotic recombination is lowered five-fold at the his1–1/his1–315 and leu1–c/leu1–12 heteroalleles. Spontaneous reversion rates of both his1–1 and his1–315 were elevated 10 to 20 fold in rad52/rad52 diploids.—The RAD52 gene function is required for spontaneous mitotic recombination, UV- and γ-ray-induced mitotic recombination and meiotic recombination. 相似文献
18.
Female parasitoids use a hierarchy of cues to locate suitable hosts. We conducted a series of field observations and experiments to examine host location behavior in Pseudacteon tricuspis Borgmeier, a phorid parasitoid of Solenopsis invicta Buren worker ants. The parasitoids were frequently attracted to host workers at disturbed colonies, but were almost never attracted to host workers foraging at baits. When conspecific nonnestmate workers were introduced to baits, resulting in aggressive interactions, parasitoids appeared at the majority of baits. Moreover, larger numbers of parasitoids appeared at baits to which greater numbers of nonnestmate workers had been added. Addition of nonnestmate workers to disturbed colonies resulted in increased numbers of parasitoids attracted. Pseudacteon tricuspis did not display a pattern of uniform distribution at disturbed colonies but often was very abundant at some colony locations while absent or rare at nearby colony locations. Solenopsis invicta workers release alarm pheromones in aggressive interactions with nonnestmates, and this substance is likely an important chemical cue that attracts P. tricuspis flies to host workers from a distance. 相似文献
19.
With two copies of every gene, a diploid organism is able to mask recessive deleterious mutations. In this paper we present the analysis of a two-locus model designed to determine when the masking of deleterious alleles favors the evolution of a dominant diploid phase in organisms that alternate between haploid and diploid phases ("alternation of generations"). It is hypothesized that diploidy will be favored whenever masking occurs ("the masking hypothesis"). Using analytical methods, we confirm that this masking hypothesis is essentially correct under free recombination: as long as the heterozygous expression of deleterious alleles is sufficiently masked by the wild-type allele, diploidy is favored over haploidy. When the rate of recombination is lower, however, diploidy is much less likely to be favored over haploidy. In fact, according to our model, the evolution of diploidy is impossible without significant levels of recombination even when masking is fairly strong. 相似文献
20.
J. Maynard Smith 《Genetics》1974,78(1):299-304