共查询到20条相似文献,搜索用时 31 毫秒
1.
Kazuaki Yoshimune Hajime Morimoto Yu Hirano Junshi Sakamoto Hidetoshi Matsuyama Isao Yumoto 《Journal of bioenergetics and biomembranes》2010,42(2):111-116
Alkaliphiles grow under alkaline conditions that might be disadvantageous for the transmembrane pH gradient (ΔpH, outside
acidic). In this study, the behaviors of extruded protons by the respiration of obligate alkaliphilic Bacillus clarkii K24-1U were investigated by comparison with those of neutralophilic Bacillus subtilis IAM 1026. Although whole-cell suspensions of both Bacillus species consumed oxygen immediately after the addition of air, there were lag times before the suspensions were acidified.
Under alkaline conditions, the lag time for B. clarkii significantly increased, whereas that for B. subtilis decreased. In the presence of valinomycin or ETH-157, which disrupts the membrane electrical potential (Δψ), the cell suspensions
of both Bacillus species acidified immediately after the addition of air. Artificial electroneutral antiporters (nigericin and monensin) that
eliminate the ΔpH exhibited no significant effect on the lag times of the two Bacillus species except that monensin increased the lag times of B. clarkii. The inhibition of ATPase and the Na+ channel also exhibited little effects on the lag times. The increased lag time for B. clarkii may represent the Δψ-dependent proton retention on the outer surface of the cytoplasmic membrane to generate a sufficient
ΔpH under alkaline conditions. 相似文献
2.
A. Brune P. Gonzalez R. Goren U. Zehavi E. Echeverria 《The Journal of membrane biology》1998,166(3):197-203
Citrate transport into the vacuoles of acid lime juice cells was investigated using isolated tonoplast vesicles. ATP stimulated
citrate uptake in the presence or in the absence of a ΔμH+. Energization of the vesicles only by an artificial K+ gradient (establishing an inside-positive Δψ) also resulted in citrate uptake as was the case of a ΔpH dominated ΔμH+. Addition of inhibitors to endomembrane ATPases showed no direct correlation between the inhibition to the tonoplast bound H+/ATPase and citrate uptake. The data indicated that, although some citrate uptake can be accounted for by Δψ and by a direct primary
active transport mechanism involving ATP, under in vivo conditions of vacuolar pH of 2.0, citrate uptake is driven by ΔpH.
Received: 27 April 1998/Revised: 8 September 1998 相似文献
3.
Blaikie FH Brown SE Samuelsson LM Brand MD Smith RA Murphy MP 《Bioscience reports》2006,26(3):231-243
The protonmotive force (Δp) across the mitochondrial inner membrane drives ATP synthesis. In addition, the energy stored in Δp can be dissipated by proton leak through the inner membrane, contributing to basal metabolic rate and thermogenesis. Increasing mitochondrial proton leak pharmacologically should decrease the efficiency of oxidative phosphorylation and counteract obesity by enabling fatty acids to be oxidised with decreased ATP production. While protonophores such as 2,4-dinitrophenol (DNP) increase mitochondrial proton leak and have been used to treat obesity, a slight increase in DNP concentration above the therapeutically effective dose disrupts mitochondrial function and leads to toxicity. Therefore we set out to develop a less toxic protonophore that would increase proton leak significantly at high Δp but not at low Δp. Our design concept for a potential self-limiting protonophore was to couple the DNP moiety to the lipophilic triphenylphosphonium (TPP) cation and this was achieved by the preparation of 3-(3,5-dinitro-4-hydroxyphenyl)propyltriphenylphosphonium methanesulfonate (MitoDNP). TPP cations accumulate within mitochondria driven by the membrane potential (Δψ), the predominant component of Δp. Our hypothesis was that MitoDNP would accumulate in mitochondria at high Δψ where it would act as a protonophore, but that at lower Δψ the accumulation and uncoupling would be far less. We found that MitoDNP was extensively taken into mitochondria driven by Δψ. However MitoDNP did not uncouple mitochondria as judged by its inability to either increase respiration rate or decrease Δψ. Therefore MitoDNP did not act as a protonophore, probably because the efflux of deprotonated MitoDNP was inhibited. 相似文献
4.
Annukka E. Mäkinen Anna H. Kaksonen Jaakko A. Puhakka 《Extremophiles : life under extreme conditions》2009,13(3):505-510
A thermophilic, rod-shaped, motile, Gram-positive, spore-forming bacterium strain 70BT was isolated from a geothermally active underground mine in Japan. The temperature and pH range for growth was 50–81°C (optimum
71°C) and 6.2–9.8 (optimum pH 7–7.5), respectively. Growth occurred in the presence 0–2% NaCl (optimum 1% NaCl). Strain 70BT could utilize glucose, fructose, mannose, mannitol, pyruvate, cellobiose and tryptone as substrates. Thiosulfate was used
as electron acceptor. Major whole-cell fatty acids were iso-C15:0, C16:0 DMA (dimethyl acetal), C16:0 and anteiso-C15:0. The G+C mol% of the DNA was 44.2%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the closest relatives
of strain 70BT were Thermosediminibacter oceani DSM 16646T (94% similarity) and Thermosediminibacter litoriperuensis DSM 16647 (93% similarity). The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain 70BT represents a novel species in a new genus, for which the name Thermovorax subterraneus gen. nov., sp. nov. is proposed. The type strain of Thermovorax subterraneus is 70BT (=DSM 21563 = JCM 15541). 相似文献
5.
Yoval-Sánchez B Jasso-Chávez R Lira-Silva E Moreno-Sánchez R Rodríguez-Zavala JS 《Journal of bioenergetics and biomembranes》2011,43(5):519-530
Ethanol is one of the most efficient carbon sources for Euglena gracilis. Thus, an in-depth investigation of the distribution of ethanol metabolizing enzymes in this organism was conducted. Cellular
fractionation indicated localization of the ethanol metabolizing enzymes in both cytosol and mitochondria. Isolated mitochondria
were able to generate a transmembrane electrical gradient (Δψ) after the addition of ethanol. However, upon the addition of
acetaldehyde no Δψ was formed. Furthermore, acetaldehyde collapsed Δψ generated by ethanol or malate but not by D-lactate.
Pyrazole, a specific inhibitor of alcohol dehydrogenase (ADH), abolished the effect of acetaldehyde on Δψ, suggesting that
the mitochondrial ADH, by actively consuming NADH to reduce acetaldehyde to ethanol, was able to collapse Δψ. When mitochondria
were fractionated, 27% and 60% of ADH and aldehyde dehydrogenase (ALDH) activities were found in the inner membrane fraction.
ADH activity showed two kinetic components, suggesting the presence of two isozymes in the membrane fraction, while ALDH kinetics
was monotonic. The ADH Km values were 0.64–6.5 mM for ethanol, and 0.16–0.88 mM for NAD+, while the ALDH Km values were 1.7–5.3 μM for acetaldehyde and 33–47 μM for NAD+. These novel enzymes were also able to use aliphatic substrates of different chain length and could be involved in the metabolism
of fatty alcohol and aldehydes released from wax esters stored by this microorganism. 相似文献
6.
In this work, high ΔμH+-dependent succinate oxidase activity has been demonstrated for the first time with membrane vesicles isolated from Bacillus subtilis. The maximal specific rate of succinate oxidation by coupled inside-out membrane vesicles isolated from a B. subtilis strain overproducing succinate:menaquinone oxidoreductase approaches the specific rate observed with the intact cells. Deenergization
of the membrane vesicles with ionophores or alamethicin brings about an almost complete inhibition of succinate oxidation.
An apparent K
m for succinate during the energy-dependent succinate oxidase activity of the vesicles (2.2 mM) is higher by an order of magnitude
than the K
m value measured for the energy-independent reduction of 2,6-dichlorophenol indophenol. The data reveal critical importance
of ΔμH+ for maintaining active electron transfer by succinate:menaquinone oxidoreductase. The role of ΔμH+ might consist in providing energy for thermodynamically unfavorable menaquinone reduction by succinate by virtue of transmembrane
electron transport within the enzyme down the electric field; alternatively, ΔμH+ could play a regulatory role by maintaining the electroneutrally operating enzyme in a catalytically active conformation. 相似文献
7.
Screening of high-yielding biocontrol bacterium Bs<Emphasis Type="Italic">-</Emphasis>916 mutant by ion implantation 总被引:1,自引:0,他引:1
Bacillus subtilis 916 was an effective biocontrol agent in control rice sheath blight caused by Rhizoctonia solani. To further improve its antagonistic ability, low-energy ion implantation was applied in Bs-916. We studied the effects of
different doses of N+ implantation. The optimum dose of ion implantation for the Bs-916 was from 15 × 2.6 × 1014 N+/cm2 to 25 × 2.6 × 1014 N+/cm2. The mutant strain designated as Bs-H74 was obtained, which showed higher inhibition activity in the screening plate. Its
inhibition zone against the indicator organism increased by 30.7% compared to the parental strain. The control effect of rice
sheath blight was improved by 14.6% over that of Bs-916. Thin-layer chromatography and high-performance liquid chromatography
analysis indicated that lipopeptides produced by Bs-916 and the mutant strains belonged to the surfactin family. Bs-H74 produced
approximately 3.0-fold surfactin compared to that of Bs-916. To determine the role of surfactin in biocontrol by Bs-916, we
tested another mutant strain, Bs-M49, which produced lower levels of surfactin significantly, and found that Bs-M49 had no
obvious effects against R. solani. These results suggested that the surfactin produced by Bs-916 plays an important role in the suppression of sheath blight.
These observations also showed that the Bs-H74 mutant strain is a better biocontrol agent than the parental strain. 相似文献
8.
Mamedov MD Tyunyatkina AA Siletsky SA Semenov AY 《European biophysics journal : EBJ》2006,35(8):647-654
An electrometrical technique was used to investigate proton-coupled electron transfer between the primary plastoquinone acceptor QA− and the oxidized non-heme iron Fe3+ on the acceptor side of photosystem II core particles incorporated into phospholipid vesicles. The sign of the transmembrane electric potential difference Δψ (negative charging of the proteoliposome interior) indicates that the iron–quinone complex faces the interior surface of the proteoliposome membrane. Preoxidation of the non-heme iron was achieved by addition of potassium ferricyanide entrapped into proteoliposomes. Besides the fast unresolvable kinetic phase (τ ∼ 0.1 μs) of Δψ generation related to electron transfer between the redox-active tyrosine YZ and QA, an additional phase in the submillisecond time domain (τ ∼ 0.1 ms at 23°C, pH 7.0) and relative amplitude ∼ 20% of the amplitude of the fast phase was observed under exposure to the first flash. This phase was absent under the second laser flash, as well as upon the first flash in the presence of DCMU, an inhibitor of electron transfer between QA and the secondary quinone QB. The rate of the additional electrogenic phase is decreased by about one-half in the presence of D2O and is reduced with the temperature decrease. On the basis of the above observations we suggest that the submillisecond electrogenic reaction induced by the first flash is due to the vectorial transfer of a proton from external aqueous phase to an amino acid residue(s) in the vicinity of the non-heme iron. The possible role of the non-heme iron in cyclic electron transfer in photosystem II complex is discussed. 相似文献
9.
Three strains of gram-positive, motile, rod-shaped and boron (B)-tolerant bacterium were isolated from naturally B containing
soil of Hisarcik area in the Kutahya Province, Turkey. The strains, designated as T-14A, T-15ZT and T-17s, produced spherical or ellipsoidal endospores in a terminal bulging sporangium. The strains required B for the
growth and can tolerate more than 450 mM B. These also tolerated up to 7.0% (w/v) NaCl in the presence of 50 mM B in agar
medium but grew optimally without NaCl. The temperature range for growth was 16–37°C (optimal of 30°C), whereas the pH range
was 6.5–9.0 (optimal of 7.5–8.5). The DNA G + C content was 41.1–42.2 mol% and the predominant cellular fatty acid was iso-C15:0. The major respiratory quinone system was detected as MK-7 and the diamino acid of the peptidoglycan was meso-diaminopimelic acid. Based on phenotypic and chemotaxonomic characteristics, phylogenetic analysis of 16S rRNA gene sequences
data and DNA–DNA re-association values, we concluded that the three strains belong to a novel species of the genus Bacillus, the type strain of which is T-15ZT and for which we proposed the name, B. boroniphilus sp. nov. (DSM 17376T = IAM 15287T = ATCC BAA-1204T). 相似文献
10.
The sweet protein monellin gene was expressed in Bacillus subtilis under the control of the Bacillus subtilis
sacB promoter and signal peptide sequence. A 294-bp DNA fragment, coding for sweet protein monellin, was ligated into the Escherichia coli/B. subtilis shuttle vector pHPC, producing pHPMS, which was subsequently transformed into B. subtilis QB1098, DB104, and DB403. The peptide efficiently directed the secretion of monellin from the recombinant B. subtilis cells. A maximum yield of monellin of 0.29 g protein l−1 was obtained from the supernatant of B. subtilis DB403 harboring pHPMS. SDS-PAGE confirmed the purity of the recombinant product. 相似文献
11.
D. Y. Sorokin T. P. Tourova A. N. Panteleeva E. N. Kaparullina G. Muyzer 《Extremophiles : life under extreme conditions》2012,16(2):307-315
Anaerobic enrichments at pH 10, with pectin and polygalacturonates as substrates and inoculated with samples of sediments
of hypersaline soda lakes from the Kulunda Steppe (Altai, Russia) demonstrated the potential for microbial pectin degradation
up to soda-saturating conditions. The enrichments resulted in the isolation of six strains of obligately anaerobic fermentative
bacteria, which represented a novel deep lineage within the order Clostridiales loosely associated with the family Lachnospiraceae. The isolates were rod-shaped and formed terminal round endospores. One of the striking features of the novel group is a
very narrow substrate spectrum for growth, restricted to galacturonic acid and its polymers (e.g. pectin). Acetate and formate
were the final fermentation products. Growth was possible in a pH range from 8 to 10.5, with an optimum at pH 9.5–10, and
in a salinity range from 0.2 to 3.5 M Na+. On the basis of unique phenotypic properties and distinct phylogeny, the pectinolytic isolates are proposed to be assigned
to a new genus Natranaerovirga with two species N. hydrolytica (APP2T=DSM24176T=UNIQEM U806T) and N. pectinivora (AP3T=DSM24629T=UNIQEM U805T). 相似文献
12.
A. R. Waldeck Karel van Dam Jan Berden P. W. Kuchel 《European biophysics journal : EBJ》1998,27(3):255-262
A non-equilibrium thermodynamics (NET) model describing the action of completely coupled or `slipping' reconstituted Ca2+-ATPase is presented. Variation of the coupling stoichiometries with the magnitude of the electrochemical gradients, as the
ATPase hydrolyzes ATP, is an indication of molecular slip. However, the Ca2+ and H+ membrane-leak conductances may also be a function of their respective gradients. Such non-ohmic leak typically yields `flow-force'
relationships that are similar to those that are obtained when the pump slips; hence, caution needs to be exercised when interpreting
data of Ca2+-ATPase-mediated fluxes that display a non-linear dependence on the electrochemical proton (Δμ˜H) and/or calcium gradients (Δμ˜Ca). To address this issue, three experimentally verifiable relationships differentiating between membrane leak and enzymic
slip were derived. First, by measuring Δμ˜H as a function of the rate of ATP hydrolysis by the enzyme. Second, by measuring the overall `efficiency' of the pump as a
function of Δμ˜H. Third, by measuring the proton ejection rate by the pump as a function of its ATP hydrolysis rate.
Received: 19 June 1997 / Accepted: 3 December 1997 相似文献
13.
In vivo studies with leaf cells of aquatic plant species such as Elodea nuttallii revealed the proton permeability and conductance of the plasma membrane to be strongly pH dependent. The question was posed
if similar pH dependent permeability changes also occur in isolated plasma membrane vesicles. Here we report the use of acridine
orange to quantify passive proton fluxes. Right-side out vesicles were exposed to pH jumps. From the decay of the applied
ΔpH the proton fluxes and proton permeability coefficients (PH+) were calculated. As in the intact Elodea plasma membrane, the proton permeability of the vesicle membrane is pH sensitive, an effect of internal pH as well as external
pH on PH+ was observed. Under near symmetric conditions, i.e., zero electrical potential and zero ΔpH, PH+ increased from 65 × 10−8 at pH 8.5 to 10−1 m/sec at pH 11 and the conductance from 13 × 10−6 to 30 × 10−4 S/m2. At a constant pH
i
of 8 and a pH
o
going from 8.5 to 11, PH+ increased more than tenfold from 2 to 26 × 10−6 m/sec. The calculated values of PH+ were several orders of magnitude lower than those obtained from studies on intact leaves. Apparently, in plasma membrane
purified vesicles the transport system responsible for the observed high proton permeability in vivo is either (partly) inactive
or lost during the procedure of vesicle preparation. The residue proton permeability is in agreement with values found for
liposome or planar lipid bilayer membranes, suggesting that it reflects an intrinsic permeability of the phospholipid bilayer
to protons. Possible implications of these findings for transport studies on similar vesicle systems are discussed.
Received: 5 April 1995/Revised: 28 March 1996 相似文献
14.
Chevrollier A Loiseau D Chabi B Renier G Douay O Malthièry Y Stepien G 《Journal of bioenergetics and biomembranes》2005,37(5):307-317
The three adenine nucleotide translocator ({ANT1} to {ANT3}) isoforms, differentially expressed in human cells, play a crucial
role in cell bioenergetics by catalyzing ADP and ATP exchange across the mitochondrial inner membrane. In contrast to differentiated
tissue cells, transformed cells, and their ρ0 derivatives, i.e. cells deprived of mitochondrial DNA, sustain a high rate of glycolysis. We compared the expression pattern
of {ANT} isoforms in several transformed human cell lines at different stages of the cell cycle. The level of {ANT2} expression
and glycolytic ATP production in these cell lines were in keeping with their metabolic background and their state of differentiation.
The sensitivity of the mitochondrial inner membrane potential (Δψ) to several inhibitors of glycolysis and oxidative phosphorylation
confirmed this relationship. We propose a new model for ATP uptake in cancer cells implicating the {ANT2} isoform, in conjunction
with hexokinase II and the β subunit of mitochondrial ATP synthase, in the Δψ maintenance and in the aggressiveness of cancer
cells. 相似文献
15.
Hirasawa K Uchimura K Kashiwa M Grant WD Ito S Kobayashi T Horikoshi K 《Antonie van Leeuwenhoek》2006,89(2):211-219
An endoglucanase was purified to homogeneity from an alkaline culture broth of a strain isolated from␣seawater and identified
here as Bacillus agaradhaerens JAM-KU023. The molecular mass was around 38-kDa and the N-terminal 19 amino acids of the purified enzyme exhibited 100% sequence
identity to Cel5A of B. agaradhaerens DSM8721T. The enzyme activity increased around 4-fold by the addition of 0.2–2.0 M NaCl in 0.1 M glycine–NaOH buffer (pH 9.0). KCl,
Na2SO4, NaBr, NaNO3, CH3COONa, LiCl, NH4NO3, and NH4Cl also activated the enzyme up to 2- to 4-fold. The optimal pH and temperature values were pH 7–9.4 and 60 °C with 0.2 M
NaCl, but pH 6.5–7 and 50 °C without NaCl; enzyme activity increased approximately 6-fold at 60 °C with 0.2 M NaCl compared
to that at 50 °C without NaCl in 0.1 M glycine–NaOH buffer (pH 9.0). The thermostability and pH stability of the enzyme were
not affected by NaCl. The enzyme was very stable to several chemical compounds, surfactants and metal ions (except for Fe2+ and Hg2+ ions), regardless whether NaCl was present or not.
* The nucleotide sequence of 16S rRNA of this strain has been submitted to DDBJ, EMBL, and GenBank databases under accession
no. AB211544. 相似文献
16.
<Emphasis Type="Italic">Acinetobacter brisouii</Emphasis> sp. nov., isolated from a wetland in Korea
Rangasamy Anandham Hang-Yeon Weon Soo-Jin Kim Yi-Seul Kim Byung-Yong Kim Soon-Wo Kwon 《Journal of microbiology (Seoul, Korea)》2010,48(1):36-39
A bacterial strain 5YN5-8T was isolated from peat layer on Yongneup in Korea. Cells of strain 5YN5-8T were strictly aerobic, Gram-negative, coccobacilli, non-spore forming, and non-motile. The isolate exhibited optimal growth
at 28°C, pH 7.0, and 0–1% NaCl. Results of 16S rRNA gene sequence analyses indicated a close relationship of this isolate
to Acinetobacter calcoaceticus (97.8% similarity for strain DSM 30006T). It also exhibited 94.4–97.8% 16S rRNA gene sequence similarities to the validly published Acinetobacter species. The value for DNA-DNA hybridization between strain 5YN5-8T and other members of the genus Acinetobacter ranged from 16 to 28%. Predominant cellular fatty acids were C18:1
ω9c, summed feature 4 containing C15:0 iso 2-OH and/or C16:1
ω7c, and C16:0. The DNA G+C content was 43.9 mol%. Phylogenetic, phenotypic, and chemotaxonomic data accumulated in this study revealed
that the isolate could be classified in a novel species of the genus Acinetobacter. The name Acinetobacter brisouii sp. nov. is proposed for the novel species, with 5YN5-8T (=KACC 11602T = DSM 18516T) as the type strain. 相似文献
17.
T. P. Storozhevykh Ya. E. Senilova T. Brustovetsky V. G. Pinelis N. Brustovetsky 《Neurochemical research》2010,35(2):323-335
KB-R7943, an inhibitor of a reversed Na+/Ca2+ exchanger, exhibits neuroprotection against glutamate excitotoxicity. Taking into consideration that prolonged exposure of
neurons to glutamate induces delayed calcium deregulation (DCD) and irreversible decrease of mitochondrial membrane potential
(Δψmit), we examined the effect of KB-R7943 on glutamate and kainate-induced [Ca2+]i and on Δψmit changes in rat cultured cerebellar granule neurons. 15 μmol/l KB-R7943 significantly delayed the onset of DCD in response
to kainate but not in response to glutamate. In spite of [Ca2+]i overload, KB-R7943 considerably improved the [Ca2+]i recovery and restoration of Δψmit after glutamate and kainate washout and increased cell viability after glutamate exposure. In resting neurons, KB-R7943 induced
a statistically significant decrease in Δψmit. KB-R7943 also depolarized isolated brain mitochondria and slightly inhibited mitochondrial Ca2+ uptake. These findings suggest that mild mitochondrial depolarization and diminution of Ca2+ accumulation in the organelles might contribute to neuroprotective effect of KB-R7943. 相似文献
18.
Mitochondrial dysfunction in platelets and hippocampi of senescence-accelerated mice 总被引:5,自引:0,他引:5
Xu J Shi C Li Q Wu J Forster EL Yew DT 《Journal of bioenergetics and biomembranes》2007,39(2):195-202
Senescence-accelerated mice (SAM) strains are useful models to understand the mechanisms of age-dependent degeneration. In
this study, measurements of the mitochondrial membrane potential (Δψm) of platelets and the Adenosine 5′-triphosphate (ATP) content of hippocampi and platelets were made, and platelet mitochondria were observed in SAMP8 (faster
aging mice) and SAMR1 (aging resistant control mice) at 2, 6 and 9 months of age. In addition, an Aβ-induced (Amyloid beta-protein)
damage model of platelets was established. After the addition of Aβ, the Δψm of platelets of SAMP8 at 1and 6 months of age were measured. We found that platelet Δψm, and hippocampal and platelet ATP content of SAMP8 mice decreased at a relatively early age compared with SAMR1. The platelets
of 6 month-old SAMP8 showed a tolerance to Aβ-induced damages. These results suggest that mitochondrial dysfunction might
be one of the mechanisms leading to age-associated degeneration in SAMP mice at an early age and the platelets could serve
as a biomarker for detection of mitochondrial function and age related disease. 相似文献
19.
Didier Alazard Manon Joseph Fabienne Battaglia-Brunet Jean-Luc Cayol Bernard Ollivier 《Extremophiles : life under extreme conditions》2010,14(3):305-312
An obligately anaerobic, spore-forming, acidophilic sulfate-reducing bacterium, strain SJ4T, was isolated from an acid mining effluent decantation pond sediment sample (pH around 3.0). Cells were Gram negative, non-motile,
curved rods occurring singly. Strain SJ4T grew at pH 3.6–5.5 with an optimum at pH 5.2. Strain SJ4T utilized H2, lactate, pyruvate, glycerol, glucose, and fructose as electron donors. Lactate and glucose were weakly used. Sulfate was
used as electron acceptors, but not sulfite, elemental sulfur, arsenate (V), and fumarate. The G + C content of genomic DNA
was 42.3 mol% (HPLC). 16S rRNA gene sequence analysis indicated that strain SJ4T belonged to the genus Desulfosporosinus within the family Peptococcaceae in the phylum Firmicutes. The level of 16S rRNA gene sequence similarity with other Desulfosporosinus species was 94.7–96.2%, D. orientis DSM 765T (similarity of 96.2%) and D. auripigmenti DSM 13351T (similarity of 95%) being its closest relatives. DNA–DNA relatedness values with D. orientis and D. auripigmenti were 16.5 and 31.8%, respectively. On the basis of phenotypic, phylogenetic, and genetic characteristics, strain SJ4T represents a novel species within the genus Desulfosporosinus, for which the name Desulfosporosinus acidiphilus sp. nov. is proposed. The type strain is SJ4T (=DSM 22704T = JCM 16185T). 相似文献
20.
Romano I Orlando P Gambacorta A Nicolaus B Dipasquale L Pascual J Giordano A Lama L 《Extremophiles : life under extreme conditions》2011,15(2):213-220
A novel haloalkaliphilic, facultative anaerobic and Gram-negative Salinivibrio-like microorganism (designated strain BAGT) was recovered from a saline lake in Ras Mohammed Park (Egypt). Cells were motile, curved rods, not spore-forming and occurred
singly. Strain BAGT grew optimally at 35°C (temperature growth range 25–40°C) with 10.0% (w/v) NaCl [NaCl growth range 6.0–16.0% (w/v)] and at
pH 9.0 (pH growth range 6.0–10.0). Strain BAGT had phosphatidylethanolamine (PEA) and phosphatidylglycerol (PG) as the main polar lipids, C16:0 (54.0%) and C16:1 (26.0%)
as the predominant cellular fatty acids and Q-8 as the major respiratory quinone. Phylogenetic analysis based on 16S rRNA
gene sequences indicated that strain BAGT was a member of Salinivibrio genus, with the highest sequence similarities of 99.1, 98.4 and 98.1% to Salinivibrio siamensis JCM 14472T, Salinivibrio proteolyticus DSM 19052T and Salinivibrio costicola subsp. alcaliphilus DSM 16359T, respectively. DNA–DNA hybridization values of strain BAGT with members of Salinivibrio genus were lower than 55.0%. DNA G + C content was 51.0 mol%. On the basis of the polyphasic taxonomic results revealed in
this study, strain BAGT should be classified as a novel species of Salinivibrio genus, for which the name Salinivibrio sharmensis sp. nov. is proposed, with the type strain BAGT (=ATCC BAA-1319T = DSM 18182T). 相似文献