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1.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1983,74(2):335-339
- 1.1. Compositional analysis of plasma membranes from rats fed nutritionally adequate diets different in fatty acid composition establishes that fundamentally different dietary fat intake results in alteration in structural lipid composition of plasma membranes in brain, liver and the intestinal mucosa.
- 2.2. Dietary differences in fatty acid intake altered the fatty acyl tail composition of plasma membrane phospholipids in brain, liver and intestinal mucosa.
- 3.3. Diet altered the phospholipid profile observed in brain synaptosomal and liver plasma membrane.
- 4.4. Feeding high vs low polyunsaturated to saturated fat diets for 7 days altered the fatty acid composition of phosphatidylcholine, phosphatidylethanolamine, sphingomyelin and mono-glucosylceramide isolated from plasma membrane of the intestinal mucosa
2.
《The International journal of biochemistry》1982,14(3):239-241
- 1.1. Feeding of rats with a 2% cholesterol diet for 6 weeks increased the serum cholesterol concentration. The activity of lecithin cholesterol acyltransferase was also increased during the feeding time.
- 2.2. The activities of aspartate aminotransferase and alanine aminotransferase remained on a constant level during the experiment on rats having cholesterol in their diet. Omitting cholesterol from the diet enhanced the activities of both enzymes and the increase in alanine aminotransferase activity was more pronounced.
- 3.3. The activity of alkaline phosphatase was on higher level during the whole experiment in the rats having cholesterol in the diet than in those fed a cholesterol-free diet.
- 4.4. Present data suggest that excluding cholesterol from the diet labilizes the membranes of hepatocytes and facilitates the release of aspartate and alanine aminotransferases in the blood.
3.
《The International journal of biochemistry》1994,26(2):181-187
- 1.1. Glycerolphosphate acyltransferase (GPAT) was solubilized from the rat liver mitochondrial membranes using sodium cholate. Dithiothreitol was necessary to stabilize the solubilized enzyme on storage.
- 2.2. Unlike the enzyme in situ in mitochondrial membranes, the solubilized mitochondrial GPAT was susceptible to inhibition by N-ethylmaleimide; a property more characteristic of the distinct microsomal form of GPAT.
- 3.3. Solubilized mitochondrial GPAT retained its very high preference for saturated acyl-CoA substrate (palmitoyl-CoA) and had no activity whatever with any tested concentration of the unsaturated substrate oleoyl-CoA.
- 4.4. Solubilization increased the affinity of mitochondrial GPAT for palmitoyl-CoA whilst decreasing the Km for glycerol phosphate.
- 5.5. After separation of liver mitochondrial outer and inner membranes and estimation of cross-contamination by appropriate markers it was concluded that the mitochondrial inner membrane contains significant GPAT activity. This was established with preparations from fed, 48 hr-starved and streptozotocin-diabetic rats.
4.
《Comparative biochemistry and physiology. A, Comparative physiology》1992,101(4):823-826
- 1.1. Adenylate cyclase activity was determined in membranes of white and brown adipose tissue (WAT and BAT, respectively) from rats fed a high-energy diet (EXP group) vs those fed a nutritionally balanced one (CON group).
- 2.2. The isoproterenol- and guanine nucleotide-induced adenylate cyclase activity in WAT membranes of EXP rats was lower than that in CON rats.
- 3.3. Relative adenylate cyclase activity in like treated BAT membranes was higher in EXP than in CON rats.
- 4.4. It is concluded that feeding high-energy diets to rats induces similar post-receptor modifications of adenylate cyclase as found in genetic obese rodents.
5.
《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1996,113(1):93-98
- 1.1. The actions of piroxicam, a nonsteroidal and noncarboxylic anti-inflammatory drug, on the metabolism of the isolated perfused rat liver were investigated. The main purpose was to verify if piroxicam is also active on glycogenolysis and energy metabolism, as demonstrated for several carboxylic nonsteroidal anti-inflammatories.
- 2.2. Piroxicam increased oxygen consumption in livers from both fed and fasted rats.
- 3.3. Piroxicam increased glucose release and glycolysis from endogenous glycogen (glycogenolysis).
- 4.4. Gluconeogenesis from lactate plus pyruvate was inhibited.
- 5.5. The action of piroxicam on oxygen consumption was blocked by antimycin A, but not by atractyloside.
- 6.6. The action of piroxicam in the perfused rat liver metabolism seems to be a consequence of its action on mitochondria.
- 7.7. It can be concluded that inhibition of energy metabolism and stimulation of glycogenolysis are not specific properties of carboxylic nonsteroidal anti-inflammatory drugs.
6.
《The International journal of biochemistry》1993,25(9):1241-1243
- 1.1. Berenil, administered to rats in vivo, promoted a decrease in liver SAMDC activity, but an increase in ODC and SAT activity.
- 2.2. Its effect on ODC was completely prevented by cycloheximide, that on SAT only partially.
- 3.3. Berenil had no effect on ODC activity in adrenalectomized rats. Adrenergic antagonists counteracted the effect of Berenil on ODC activity.
- 4.4. Polyamine content was increased. The maximum modification was observed for putrescine and N1-acetylspermidine.
7.
《The International journal of biochemistry》1994,26(9):1139-1145
- 1.1. Lipoperoxidation (LPx) and glutathione peroxidase (GPx) activity were measured in kidney, liver, heart, lung, brain and testis from control and puromycin aminonucleoside (PAN) injected rats on days 1–6, 8, 10, 16 and 22 after vehicle or PAN injection.
- 2.2. PAN-injected rats developed proteinuria on day 3.
- 3.3. In PAN-injected rats: (a) LPx increased in kidney, liver, lung, brain and testis before day 3 and in heart on day 3; (b) GPx activity increased in kidney, liver, heart, lung and testis and diminished in brain on day 3 or after.
8.
《Comparative biochemistry and physiology. A, Comparative physiology》1992,101(2):407-411
- 1.1. Pyruvate dehydrogenase complex (PDC) activity was measured in several tissues of rats fed for 7 or 15 days on control, or high-sucrose or high-fat diets.
- 2.2. Total activity in adipose tissue increased in the three groups 3–4-fold as compared with chow-fed animals in the first week. Total activity was 60% lower in rats fed the diet containing 22% corn oil for 2 weeks.
- 3.3. Hepatic total and PDCa activities were 50–80% higher in rats fed the sucrose diet for 7 or 15 days and decreased 30–40% in those fed on the high-fat diet for 2 weeks.
9.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1984,77(2):167-171
- 1.1. Metabolic rates and adenine nucleotide content of liver and kidney from hibernating ground squirrels were measured and compared to rats to study the biochemical adaptation to hibernation.
- 2.2. High rates of renal and hepatic gluconeogenesis were observed in squirrels, particularly from propionate and glycerol compared to rat.
- 3.3. During hibernation and starvation soluble phosphoenolpyruvate carboxykinase activity was increased in both liver and kidney.
- 4.4. Although metabolic rates are decreased during hibernation the results suggest that the enzymic complement is maintained at high activity even during torpor.
10.
《Comparative biochemistry and physiology. B, Comparative biochemistry》1983,74(2):341-344
- 1.1. The activities of β-glucuronidase and cathepsin D and the protein concentration were assayed from brain, kidney, liver, cardiac muscle and skeletal muscle (m. rectus femoris) samples from mice (Mus musculus) 1, 3, and 6 days after intermittent exhaustive (duration 100–145min) and submaximal prolonged (duration 9 hr) running on treadmill.
- 2.2. The activity of β-glucuronidase in skeletal muscle strongly increased being the highest 3 days after both exertions. Cathepsin D activity also slightly increased. In cardiac muscle β-glucuronidase activity was unaffected. Cathepsin D activity slightly increased 3 days after intermittent exhaustive exercise.
- 3.3. The specific activities of β-glucuronidase and cathepsin D in the liver increased 1 day after the both exertions. Simultaneously the protein concentration decreased. In the kidney β-glucuronidase activity and protein concentration were unaffected but cathepsin D activity decreased 1 day after intermittent exhaustive exercise.
- 4.4. In the brain protein concentration transiently decreased 3 days after the exertions. β-Glucuronidase activity transiently decreased 1 day after intermittent exercise thereafter increasing 6 days afterwards above the control level. Cathepsin D activity decreased 1 day after intermittent exercise but was unaffected after prolonged submaximal exercise.
- 5.5. Physical stress affected to varying extent the acid hydrolase activities in all organs studied.
11.
《Comparative biochemistry and physiology. A, Comparative physiology》1984,77(3):459-462
- 1.1. Rainbow trout were fed either graded levels of lysine (0.8, 1.8 and 3%) at a constant level of arginine (1.4%) or excess arginine (2.4%) at a fixed level of lysine (1.8%).
- 2.2. Increasing the dietary lysine level affected plasma urea, plasma arginine and ammonia excretion.
- 3.3. Trout fed graded levels of lysine received an arginine challenge (U14C-l-arginine) and it was found that excess dietary lysine led to a decrease in arginine degradation.
- 4.4. Injection of l-lysine induced a decrease in urea excretion, while injection of l-arginine increased both urea and ammonia excretion in control well-fed trout.
- 5.5. These results are discussed in the light of current knowledge on the antagonism between lysine and arginine.
12.
《The International journal of biochemistry》1993,25(2):223-232
- 1.1. The feeding of 0.5% (3,5,5-trimethylhexanoyl)ferrocene (TMH-ferrocene) in rats resulted in a severe and progressive liver siderosis (total liver iron, 30 mg/g liver wet weight, after 30 weeks).
- 2.2. High concentrations of an iron-rich ferritin (up to 250 mg/l) were detected in serum of heavily iron-loaded rats forming a large fraction of non-transferrin-bound-iron (5000 μg/dl in maximum).
- 3.3. Ferritin and not haemosiderin was the major iron storage protein in the liver.
- 4.4. The total liver iron concentration (from 0.4 to > 30 mg Fe/g wet wt) but not the cytosolic low-molecular-weight-iron fraction (from 0.5 to 2.5 μM) was extremely increased during iron-loading.
13.
《The International journal of biochemistry》1984,16(12):1345-1347
- 1.1. The effect of diabetes on some enzymes of polyamine metabolism was studied in male rats 1–12 days after administration of streptozotocin.
- 2.2. Hepatic ornithine decarboxylase activity decreased in the first days after the administration, but increased thereafter. The decrease was not due to an alteration of the ODC-antizyme concentration, nor to a posttranslational modification catalyzed by transglutaminase.
- 3.3. S-adenosylmethionine decarboxylase and ornithine transaminase were both increased.
- 4.4. Spermicline acetyltransferase activity was practically unchanged, while its inactivating factor was markedly decreased.
14.
《Comparative biochemistry and physiology. A, Comparative physiology》1993,104(4):831-836
- 1.1. Administration of a carbohydrate-rich diet increased haemolymph glucose levels and glycogen concentration in hepatopancreas, mantle and muscle.
- 2.2. Glycogen concentration in tissues decreases after 2 weeks of starvation and haemolymph glucose levels did not change significantly.
- 3.3. However, starvation did not induce a decrease in the intrinsic synthetic capacity in tissues.
- 4.4. Glycogen synthesis in tissues from animals fed with lettuce or a carbohydrate-rich diet, increases with increasing glucose concentration in the media.
- 5.5. However, in mantle slices from snails adapted on a carbohydrate-rich diet, the glycogen synthetic capacity was lower than in slices from snails fed with lettuce.
15.
《The International journal of biochemistry》1990,22(5):453-460
- 1.1. Purified native rabbit liver phosphorylase kinase becomes activated during the assay of its activity while low molecular weight forms of the same enzyme do not.
- 2.2. The activation requires ATP and maganesium ions, suggesting the phosphorylation of the enzyme by a protein kinase as the mechanism involved.
- 3.3. The activation of the enzyme can be reverted by the action of a type 1 protein phosphatase isolated from the same tissue.
- 4.4. The activation can also be catalyzed by the catalytic subunit of cAMP-dependent protein kinase in a process that requires a much lower ATP concentration to proceed.
- 5.5. The activation is believed to be due to an autocatalytic phosphorylation of phosphorylase kinase itself. In support of this hypothesis are the regulation of the process through calcium ions, the low levels of endogenous protein kinase detected in the purified preparation, the high ATP concentrations required in the absence of cAMP dependent protein kinase and the fact that the process cannot be blocked by an excess of the heat stable inhibitor specific for the later enzyme.
- 6.6. The low molecular weight forms of the enzyme on their side are not affected by the action of neither protein phosphatase 1 nor cyclic AMP dependent protein kinase.
- 7.7. Both activated and nonactivated phosphorylase kinase are partially dependent on calcium ions, the affinity of the former being higher than that of the latter. The low molecular forms do not require calcium ions to express their activity.
16.
- 1.1. The overall effect of handling, anaesthesia and sham injection on some blood metabolites, liver glycogen and several key enzymes involved in liver carbohydrates and nitrogen metabolism was studied in rainbow trout. In addition, the possible role of anaesthesia (MS222) itself as a stress-inductor or suppressor was also studied.
- 2.2. Stress resulted in hyperglycaemia and initially in liver glycogen depletion, as well as increasing plasma amino acid levels.
- 3.3. Glycogen stores subsequently recovered while amino acid concentration fell.
- 4.4. These changes seemed to correlate with the increased activity of liver fructose 1,6-bisphosphatase, glucose 6-phosphate dehydrogenase, alanine aminotransferase and glutamate dehydrogenase, thus supporting the hypothesis that gluconeogenic flux from amino acids increases in stressed trouts.
- 5.5. Anaesthesia, under the same experimental conditions, did not seem to mediate in stress production, but rather resulted in stress suppression.
17.
《The International journal of biochemistry》1994,26(6):777-785
- 1.1. Stearoyl-CoA desaturase (Δ9-desaturase) activity was measured in chicken primary hepatocytes, as a function of time in culture.
- 2.2. When using fasted donor animals, the desaturase activity was low at the beginning of culture and then increased steadily to a maximum value between 30 and 70 hr of culture. When hepatocyte cultures were prepared from fed animals, enzyme activity was high at the beginning of culture and maintained thereafter at similar values to those obtained in cultured hepatocytes from fasted animals after 30 hr of culture.
- 3.3. Insulin significantly enhanced enzyme activity when added to the culture medium at a 10−9M concentration, and a small stimulating effect was also observed with 10−6M dexamethasone.
- 4.4. Linoleic acid (0.5 mM) added to the culture medium as albuminic complex partly inhibited Δ9-desaturase activity.
- 5.5. Cordycepin (3' deoxyadenosine) decreased enzyme activity when present at a 3 μg/ml concentration in the culture medium.
- 6.6. Taken together, the induction of enzyme activity in culture, its impairment by cordycepin and response to insulin and linoleic acid strongly suggest that synthesis and translation of the Δ9-desaturase mRNA occur in chicken hepatocytes in primary culture, and that this cellular model may be a useful tool for further studies on Δ9-desaturase regulatory mechanisms.
18.
《Comparative biochemistry and physiology. A, Comparative physiology》1981,68(2):187-198
- 1.1. In liver and muscle the concentrations of free amino acids (FAA) are highest in fish maintained at low temperature and fed mealworms. These effects are more pronounced in roach than in rudd.
- 2.2. In the liver alanine, glycine and glutamate are the dominant FAA but proline increases in mealworm-fed animals.
- 3.3. In muscle, histidine and glycine dominate, except that a mealworm diet leads to an increase in the concentration of proline and to a concomitant decrease in the concentration of glycine.
- 4.4. Starvation leads to a reduction of total FAA content but to relative increases of lysine and histidine. These two FAA can serve as indicators of the general state of nutrition of roach and rudd.
- 5.5. The molar ratio [gly]/[his] is strongly correlated with temperature, decreasing with an increase in the temperature to which the animals had been exposed prior to capture.
- 6.6. The patterns of free and bound amino acids diverge more widely in these species than in mammals which reflects the greater dependence of the FAA pools of fish on intrinsic and extrinsic factors.
- 7.7. The concentrations of histidine in the FAA pools of muscle and in food proteins are strongly correlated.
19.
《The International journal of biochemistry》1976,7(3-4):153-157
- 1.1. Hepatic tyrosine aminotransferase activity from adult rat can be resolved into four components on hydroxylapatite column.
- 2.2. A similar profile of enzyme distribution can be obtained from late foetal liver.
- 3.3. Insulin administration to pregnant rats result in induction of two isoenzymes of tyrosine aminotransferase in foetal rat liver. Similarly Cyclic AMP injection to foetal rats in utero results in the induction of the same two forms of the enzyme.
- 4.4. Triaminolone injection to foetal rats in utero leads to the induction of three of the isoenzymes of tyrosine aminotransferase.
20.
《The International journal of biochemistry》1981,13(1):121-124
- 1.1. The amount of single-copy DNA sequences transcribed in normal tissues of adult rats (brain and liver) and in the Guerin ascites tumor (GAT) was determined by hybridization of in vitro labelled 125I-single-copy rat DNA with a vast excess of total nuclear RNA to very high Rot values (up to 350,000).
- 2.2. The tissue specificity of total nuclear RNA (nRNA) was estimated by annealing of single-copy DNA to a mixture of nuclear RNAs of two different organs (brain + GAT; liver + GAT).
- 3.3. Liver and GAT RNAs annealed to about 12% of the single-copy DNA.
- 4.4. Hybridization with a mixture of the two RNAs increased slightly the amount of hybridization.
- 5.5. In contrast to other tissues, brain nuclear RNA hybridized to a much higher level (20% of the single copy DNA). Addition of GAT RNA did not increase this value.