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影响枣试管苗生长分化的因素(简报) 总被引:8,自引:0,他引:8
骏枣试管苗在生长与分化过程中,以MS培养基的营养水平,温度25 ̄27℃为适宜,外植体是茎段比茎端好,斜剪和斜插对生长分化有利,生长素为0.3 ̄0.5mg/L为宜,细胞分裂素(6-BA)以1 ̄1.5mg/L为宜,两者最佳配比为1:3。 相似文献
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藏红花愈伤组织诱导及其细胞培养的研究 总被引:4,自引:0,他引:4
藏红花幼叶愈伤组织的诱导频率在MS,B5和White三种培养基上均高达98%;幼叶和芽的诱导率差异不大,高达99%;不同时期的叶片差异较大,以幼叶诱导为佳;球茎诱导率为近80%;激素配比以2.4-D2.0mg/L,BAP0.1~0.5ml/L为宜。在继代培养阶段,MS比B5和White更适合细胞的快速生长繁殖;并且以叶片的愈伤组织生长较快,芽次之,球茎最慢,适合于细胞生长的激素配比为NAA2.0~3.0mg/L,BAP0.5~1.0mg/L为宜。 相似文献
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唐菖蒲球茎芽的离体培养及快速繁殖 总被引:4,自引:0,他引:4
将带1-2个芽眼的唐菖蒲球茎切块接种到附加1.0mg/L BA的MS基本培养基上可诱导休眠芽萌动。无菌芽转移至附加3.0mg/L BA的培养基上可分化产生丛生芽。丛生芽的幼代增殖宜采用附加1.5mg/L BA的培养基生根培养,以MS+NAA0.1-0.5mg/L或MS+IBA1.mg/L效果最佳。 相似文献
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植物组织粗汁液中的番木瓜环斑病毒的ELISA检测技术 总被引:14,自引:0,他引:14
本研究建立和改进了检测番木瓜和西葫芦组织粗汁液里的番木瓜环斑病毒(PRV)的DAC-ELISA法和Dot-ELISA法。用不同的ELISA方法来检测不同寄主植物粗汁液里的PRV,其所用的合适的制备粗汁液的缓冲液是不同的。用DAC-ELISA法检测西葫芦粗汁液时,以0.5mol/L磷酸盐缓冲液(pH7.5,内含0.1mol/L乙二胺四乙酸二钠)为宜;而检测番木瓜粗汁液时,则还要加入0.25mol/L脲。用Dot-ELISA法检测时,在上述磷酸盐缓冲液中加入2%聚乙烯吡咯烷铜能提高对西葫芦粗汁液的检测效果。应用合适的制备粗汁液的缓冲液,DAC-ELISA法和Dot-ELISA法的灵敏度分别提高到1/4096和1/1024(稀释度)。本研究还表明,影响DAC-ELISA法的定过测定的主要因素是粗汁液的稀释度和包被液(0.05mol/L碳酸盐缓冲液,pH9.6)的用过。在较高粗汁液稀释度和包被液的用量相同时,粗汁液里的病毒含量与DAC-ELISA法的OD492nm值呈真实的线性关系。 相似文献
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锌对离体鲤鱼头呼吸的抑制作用 总被引:6,自引:0,他引:6
本工作基于化学纤维工厂废水中硫酸锌含量的石超标而设计。采用改进的离体鱼头灌注法,以不同浓度的ZnSO4.7H2O对84个鲤鱼头进行了人工灌流,结果表明,锌的渔业水质安全下限似应以0.0023mg/L(我国现行规定锌的渔业水质标准为0.1mg/L)为宜。 相似文献
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云南红豆杉愈伤组织诱导和组织培养 总被引:8,自引:0,他引:8
云南红豆杉愈伤组织的诱导频率为White〉MS〉B5,其中最高达80%,叶片和种子之间差异不大,但不同时期的叶片差异较大,以嫩叶诱导为佳,激素配比以2,4-D2.0-3.0mg/L,BAP0.5-1.0mg/L为宜,在继代培养阶段,B5比White和MS更适合细胞的快速生长、繁殖,并且有利于克服组织的褐化,适合于细胞生长的激素配比也以2,4-D2.0-3.0mg/L,BAP0.5-1.0mg/Lo 相似文献
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香艳梨离体培养研究 总被引:2,自引:0,他引:2
本试验以香艳梨的茎尖,不带芽茎段,带芽茎段,叶片,叶柄为试材,以1/2MS为基本培养基,分别附加0.5~2.0mg/L的6-卡基氨基嘌呤(6-BA)和0.1mg/L的萘乙酸(NAA)、吲哚乙酸(IAA),2,4-二氯苯氧乙酸(2,4-D)进行离体培养研究。结果表明,试材用0.1%HgCl2灭菌5~6分钟为宜;在7月份接种感染率低,愈伤组织形成较快;茎尖,不带芽茎段,带芽茎段,叶片均可作为离体培养的外植体,叶片的脱分化,分化的效果尤为明显,叶柄不宜作外植体;1/2MS+1.5mg·L-16-BA+0.1mg·L-1NAA有利于脱分化;1/2MS+1.0mg·L-16-BA有利于分化;瓶外生根优于瓶内生根。本试验可为香艳梨的工厂化育苗提供参考。 相似文献
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目的克隆人RHD基因,并对其进行鉴定。方法以RhD阳性志愿者骨髓为材料,用TRIzol试剂提取总RNA;设计、合成人RHD基因扩增引物,RT-PCR方法扩增RHD基因片段;T/A克隆后将其亚克隆人pET28a(+)载体中,经酶切、PCR和测序对重组质粒进行鉴定。结果骨髓总RNA被成功提取;RT-PCR成功扩增出RHD基因片段,其大小与预期约509bp基本一致;T/A克隆后再将其亚克隆,通过酶切和PCR证明RHD基因成功亚克隆入pET28a(+)载体中;基因测序结果比对显示,与已公布的RHD基因(GenBank登录号为NM016124)序列基本一致,同源性为98%。结论成功克隆了RHD基因,这将为进一步研究奠定基础。 相似文献
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Massatoshi Kondoh Ayumi Murakami Chiaki Shigeta Shizufumi Tanimoto 《Plant Growth Regulation》1999,28(2):107-116
The flower-inducing and -inhibiting activities of phloem exudate (PE) prepared from cotyledons of Pharbitis seedlings were examined, using apex cultures in vitro from Pharbitis as a bioassay system.The PE was prepared from photoperiodically-induced cotyledons (SD-PE). The SD-PE was subjected to the following fractionations: When the SD-PE was extracted with CHCl3 and then ethyl acetate, the inducing activity was located in the final aqueous fraction. The activity was localized in the diffusate when the aqueous fraction was dialyzed (molecular weight cut off was 10,000). The diffusate was fractionated by ion exchange chromatography, and flower-inducing activity was found in the fraction adsorbed onto anion exchange resin. When the fraction was applied to a Sep-Pak C18 cartridge, the activity eluted with 25% MeOH. As a result of the above fractionation, activity was increased about 30-fold.The nature of the flower-inhibiting activity of the PE taken from cotyledons exposed to continuous-light conditions was examined (CL-PE). The inhibiting activity was decreased as the cotyledons were exposed to longer dark periods; it appeared to be heat-stable. The CL-PE also inhibited flowering in Lemna. The CL-PE was subjected to the following fractionations: When the CL-PE was extracted with CHCl3 and ethyl acetate, activity was located in the final aqueous fraction. Activity was localized in the diffusate when the aqueous fraction was dialyzed (molecular weight cut off was 10,000). When the diffusate was fractionated by ion exchange chromatography, the activity was found in the flow-through fraction. When the fraction was applied to a hydroxyapatite cartridge, the activity eluted with 25 mM sodium phosphate buffer. When the fraction was re-dialyzed (molecular weight cut off was 1,000), the diffusate contained the activity. As a result of the above fractionation, activity was increased about 10-fold. 相似文献
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逆相蒸发法制备茶多酚脂质体及质量评价 总被引:1,自引:0,他引:1
采用逆相蒸发法制备茶多酚脂质体并进行质量评价。通过二次回归旋转组合设计优化茶多酚脂质体制备工艺及配方,对其形态、结构、粒径分布等性质进行考察。研究结果表明,最佳配方为m(大豆卵磷脂):m(胆固醇)=3:1、茶多酚质量浓度为7mg/mL、V(有机相):V(水相)=4:1、磷酸盐缓冲液浓度15mmoL/L,此条件下包封率为50.37%;所制备的茶多酚脂质体形态呈圆球形或椭球形,为大单室脂质体,有效粒径为165.3nm,Zeta电位为-69.3mV。逆相蒸发法制备茶多酚脂质体方法简单可行,所制备的脂质体具有一定缓释性。 相似文献
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枯草芽胞杆菌微生态制剂的研制 总被引:4,自引:0,他引:4
采用液体发酵工艺,确定枯草芽胞杆菌的最适发酵条件为:发酵温度30℃,初始pH值7.2,并以1%海藻酸钠和3%明胶组成的混合胶体溶液为囊壁材料,以4%氯化钙作固化剂将枯草芽胞杆菌制成微胶囊剂,稳定性试验结果显示经微胶囊包埋的枯草芽胞杆菌制剂,室温下保存1个月,活菌存活率为98.8%,保存3个月,活菌存活率为50.6%,保存6个月,活菌存活率为15.7%,均高于未经微胶囊化的样品;在4℃冷藏下保存3个月,未经微胶囊化的样品活菌存活率仅为经微胶囊包埋制剂的66.2%。该微胶囊制剂提高了活菌存活率,延长了活菌常温保存期。 相似文献
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经硫酸铵分级沉淀,离子交换层析和凝胶过滤等步骤,从人肝中获得了PAGE单一条带的谷胱甘肽过氧化物酶,比活提高120倍,得率为25%。凝胶过滤法测得分子量为90980,SDS-PAGE测定亚基分子量为22423.原子吸收法测得每分子酶含有四个硒原子。等电聚焦显示该酶等电点为5.0.酶活力的最适pH为8.5,最适温度为37℃。动力学实验提示该酶作用机理属于乒乓机制型。 相似文献
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β-葡萄糖苷酶在酿酒酵母表面的表达 总被引:1,自引:0,他引:1
应用表面表达技术对来自Trichodermareesei的β-葡萄糖苷酶在酿酒酵母表面的表达及后期性质进行了研究。实验结果表明酵母表面表达酶有活性,该酶的最佳诱导时间为24h,最适温度是70℃,而酶活的最适pH是5.5。使异源表面表达了Bgl1的酵母在以纤维二糖为唯一碳源的培养基中生长,发酵结果表明纤维二糖被明显利用了,但在培养186h后,发酵液中仍残留一定量的纤维二糖。这种技术对纤维素发酵系统中纤维二糖酶活性低的现状有所帮助。 相似文献
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The native Goα was purified from bovine brain cortex and palmitoylated in vitro. The in vitro palmitoylation site was the same as that in vivo. The internal palmitoylation of purified native Goα was found to be largely maintained. The apparant palmitoylation ratio was significantly increased after the Goa was treated with DTT. The GTPg S binding characteristic of Goα was not influenced by palmitoylation, however, the affinity for LUVs was increased dramatically. The in vitro palmitoylation model of Goα provides a better basis for studying the functional role of G protein palmitoylation in signal transduction. 相似文献