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1.
Mapping of SMV resistance gene Rsc-7 by SSR markers in soybean 总被引:8,自引:0,他引:8
Soybean mosaic virus (SMV) is one of the most prevalent pathogens that limit soybean production. In this study, segregation
ratios of resistant plants to susceptible plants in P1, P2, F1, F2 populations of Kefeng No. 1 (P1)×Nannong 1138-2 (P2) and derived RIL populations, were used to study the inheritance of resistance to the SMV strain SC-7. Populations Kefeng
No. 1 and F1 were found to be completely resistant to this SMV strain while Nannong 1138-2 was susceptible to it. The F2 and RIL populations segregated to fit a ratio of 3:1 and 1:1for resistant plants to susceptible ones, respectively. These
results indicated that a single dominant gene, designated as Rsc-7, controlled resistance to the SMV strain SC-7 in Kefeng No.1. SSR markers were used to analyze the RIL population and MAPMAKER/EXP
3.0b was employed to establish linkage between markers and this resistance gene. Combining the data of SSRs and resistance
identification, a soybean genetic map was constructed. This map, covering 2625.9 cM of the genome, converged into 24 linkage
groups, consisted of 221 SSR markers and the resistance gene Rsc-7. The Rsc-7 gene was mapped to the molecular linkage group G8-D1b+W. SSR markers Satt266, Satt634, Satt558, Satt157, and Satt698 were
found linked to Rsc-7 with distances of 43.7, 18.1, 26.6, 36.4 and 37.9 cM, respectively. 相似文献
2.
Jeffery D. Ray Wilfrido Morel James R. Smith Reid D. Frederick Monte R. Miles 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(2):271-280
Two soybean accessions, PI 587886 and PI 587880A, previously identified as having resistance to Phakospora pachyrhizi Syd. (soybean rust, SBR) were used to create two populations (POP-1 and POP-2) segregating for SBR resistance. F2-derived F3 (F2:3) families from each population were grown in a naturally SBR-infected field in Paraguay to determine inheritance and map
resistance genes. Over 6,000 plants from 178 families in POP-1 and over 5,000 plants from 160 families in POP-2 were evaluated
at R5 for lesion type: immune reaction (IR), reddish-brown (RB), or tan (TAN) colored lesions. Based on the lesion type present,
each F2:3 family was rated as resistant, segregating or susceptible and this classification was used to infer the F2-phenotype and genotype. For both populations, the F2 segregation ratios fit a 1:2:1 (resistant:segregating:susceptible) ratio expected for a single gene (P > 0.05). The RB lesions occurred almost exclusively in the heterozygous class, indicating incomplete dominance under the
conditions of this study. Molecular markers flanking the locations of the known resistance genes were used to map the resistance
gene in both populations to the Rpp1 locus. However, evaluation of PI 587886 and PI 587880A against eight P. pachyrhizi isolates indicated that the resistance allele in these two accessions was different from Rpp1. This test also demonstrated that these accessions were resistant to at least one P. pachyrhizi isolate collected in the southern US. This is the first report of using an adult plant field-screen with natural rust pressure
to map SBR resistance. 相似文献
3.
James D. Shull Cynthia M. Lachel Clare R. Murrin Karen L. Pennington Beverly S. Schaffer Tracy E. Strecker Karen A. Gould 《Mammalian genome》2007,18(9):657-669
Estrogens are important regulators of growth and development and contribute to the etiology of several types of cancer. Different
inbred rat strains exhibit marked, cell-type-specific differences in responsiveness to estrogens as well as differences in
susceptibility to estrogen-induced tumorigenesis. Regulation of pituitary lactotroph homeostasis is one estrogen-regulated
response that differs dramatically between different inbred rat strains. In this article we demonstrate that the growth response
of the anterior pituitary gland of female ACI rats to 17β-estradiol (E2) markedly exceeds that of identically treated female
Brown Norway (BN) rats. We further demonstrate that pituitary mass, a surrogate indicator of absolute lactotroph number, behaves
as a quantitative trait in E2-treated F2 progeny generated in a genetic cross originating with BN females and ACI males. Composite interval mapping analyses of the
(BN×ACI)F2 population revealed quantitative trait loci (QTLs) that exert significant effects on E2-induced pituitary growth on rat chromosome
4 (RNO4) (Ept5) and RNO7 (Ept7). Continuous treatment with E2 rapidly induces mammary cancer in female ACI rats but not BN rats, and QTLs that impact susceptibility
to E2-induced mammary cancer in the (BN×ACI)F2 population described here have been mapped to RNO3 (Emca5), RNO4 (Emca6), RNO5 (Emca8), RNO6 (Emca7), and RNO7 (Emca4). Ept5 and Emca6 map to distinct regions of RNO4. However, Ept7 and Emca4 map to the same region of RNO7. No correlation between pituitary mass and mammary cancer number at necropsy was observed
within the (BN×ACI)F2 population. This observation, together with the QTL mapping data, indicate that with the exception of the Ept7/Emca4 locus on RNO7, the genetic determinants of E2-induced pituitary growth differ from the genetic determinants of susceptibility
to E2-induced mammary cancer. 相似文献
4.
P. J. Rayapati J. W. Gregory M. Lee R. P. Wise 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(7-8):831-837
An F2 oat population was produced by crossing the diploid (n=7) species Avena strigosa (CI 3815) with A. wiestii (CI 1994), resistant and susceptible, respectively, to 40 isolates of Puccinia coronata, the causal agent of crown rust. Eighty-eight F2 individuals were used to construct an RFLP linkage map representing the A genome of cultivated hexaploid oat. Two hundred and eight RFLP loci have been placed into 10 linkage groups. This map covers 2416 cM, with an average of 12 cM between RFLP loci. Eighty-eight F3 lines, derived from F2 individuals used to construct the map, were screened for resistance to 9 isolates of P. coronata. One locus, Pca, was found to confer a dominant resistance phenotype to isolates 203, 258, 263, 264B, 290, 298, 325A, and 345. Pca also conferred resistance to isolate 276; however, an unlinked second gene may also be involved.Journal Paper No. 15143 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa. Project No. 3134 and 2447 相似文献
5.
Jirapong Jairin Kittiphong Phengrat Sanguan Teangdeerith Apichart Vanavichit Theerayut Toojinda 《Molecular breeding : new strategies in plant improvement》2007,19(1):35-44
The brown planthopper (BPH) is one of the most destructive insect pests of rice in Thailand. We performed a cluster analysis
that revealed the existence of four groups corresponding to the variation of virulence against BPH resistance genes in 45
BPH populations collected in Thailand. Rice cultivars Rathu Heenati and PTB33, which carry Bph3, showed a broad-spectrum resistance against all BPH populations used in this study. The resistant gene Bph3 has been extensively studied and used in rice breeding programs against BPH; however, the chromosomal location of Bph3 in the rice genome has not yet been determined. In this study, a simple sequence repeat (SSR) analysis was performed to identify
and localize the Bph3 gene derived from cvs. Rathu Heenati and PTB33. For mapping of the Bph3 locus, we developed two backcross populations, BC1F2 and BC3F2, from crosses of PTB33 × RD6 and Rathu Heenati × KDML105, respectively, and evaluated these for BPH resistance. Thirty-six
polymorphic SSR markers on chromosomes 4, 6 and 10 were used to survey 15 resistant (R) and 15 susceptible (S) individuals
from the backcross populations. One SSR marker, RM190, on chromosome 6 was associated with resistance and susceptibility in
both backcross populations. Additional SSR markers surrounding the RM190 locus were also examined to define the location of
Bph3. Based on the linkage analysis of 208 BC1F2 and 333 BC3F2 individuals, we were able to map the Bph3 locus between two flanking SSR markers, RM589 and RM588, on the short arm of chromosome 6 within 0.9 and 1.4 cM, respectively.
This study confirms both the location of Bph3 and the allelic relationship between Bph3 and bph4 on chromosome 6 that have been previously reported. The tightly linked SSR markers will facilitate marker-assisted gene pyramiding
and provide the basis for map-based cloning of the resistant gene. 相似文献
6.
Joseph M. Cash Elaine F. Remmers Ellen A. Goldmuntz Leslie J. Crofford Hongbin Zha Carl T. Hansen Ronald L. Wilder 《Mammalian genome》1993,4(1):37-42
The nude trait in the rat is transmitted in an autosomal recessive manner and is associated with thymic aplasia, T-cell deficiency, and hairlessness. Congenic rats homozygous for the RNU (Rowett nude) locus are important models in the study of inflammatory disease, tumor growth, and transplant rejection. The RNU locus has not been previously mapped, and the nature of the gene product is unknown. To determine the map location of this gene, a single F344.rnu/rnu (athymic nude congenic Fischer rat) male congenic rat was bred with 3 LEW/N (NIH stock Lewis rat) female rats to produce F1 progeny. Twelve F1 brother-sister breeding pairs were established. Forty-nine phenotypically nude F2 offspring (198 total) were obtained. Linkage analysis done on F2 DNA revealed highly significant cosegregation between the nude phenotype and eight polymorphic markers located on Chromosome (Chr) 10. The tightest linkages were with: MYH3 (embryonic, skeletal myosin heavy chain) and SHBG (sex hormone-binding globulin), giving 2 point lod scores of 20.2, and 20.0, respectively. The map order and map distances, determined by multipoint linkage calculations, were: RR24-(16.1 cM)-MYH3-(3.5 cM)-SHBG-(4.7 cM)-RNU-(11.9 cM)-F16F2-(24.1 cM)-CLATP (citrate lyase ATPase)-(2.4 cM)-ACE (angiotensin converting enzyme)/PPY (pancreatic polypeptide)-(14.1 cM)-RR1023. The position of the RNU locus in the rat corresponds closely with that of the recently reported nu locus in the mouse. This finding suggests that the nude phenotype in the rat and the mouse arise from defects in homologous genes. 相似文献
7.
Mapping and confirmation of a new sudden death syndrome resistance QTL on linkage group D2 from the soybean genotypes PI 567374 and ‘Ripley’ 总被引:1,自引:0,他引:1
Austeclinio L. de Farias Neto Rizwan Hashmi Michael Schmidt Shawn R. Carlson Glen L. Hartman Shuxian Li Randall L. Nelson Brian W. Diers 《Molecular breeding : new strategies in plant improvement》2007,20(1):53-62
The use of resistant cultivars is the most effective method for controlling sudden death syndrome (SDS), caused by Fusarium solani f. sp. glycines (FSG) (syn. Fusarium virguliforme Akoi, O’Donnell, Homma and Lattanzi), in soybean [Glycine max (L.) Merr.]. Previous research has led to the identification of soybean genotypes with partial resistance to SDS and quantitative
trait loci (QTL) controlling this resistance. The objective of our study was to map QTL conferring SDS resistance in populations
developed from the crosses Ripley × Spencer (R×S-1) and PI 567374 × Omaha (P×O-1). Both Ripley and PI 567374 have partial
resistance to SDS and Spencer and Omaha are susceptible. The R×S-1 population was evaluated for SDS resistance in three field
environments and the P×O-1 population was greenhouse evaluated. Three SDS resistance QTL were mapped in the R×S-1 population
and two in the P×O-1 population. One resistance QTL was mapped to the same location on linkage group (LG) D2 in both backgrounds.
This QTL was then tested in a population of F2 plants developed through one backcross (BC1F2) in the PI 567374 source and in a population of F8 plants derived from a heterozygous F5 plant in the Ripley source. The LG D2 QTL was also significant in confirmation populations in both resistant backgrounds.
Since none of the SDS resistance QTL identified in the R×S-1 or P×O-1 populations mapped to previously reported SDS resistance
regions, these new QTL should be useful sources of SDS resistance for soybean breeders. 相似文献
8.
J. E. CRANHAM 《The Annals of applied biology》1982,100(1):25-38
Assays on eggs of Panonychus ulmi held on leaf discs of Myrobalan plum were used to characterise strains (1) multi-resistant to organophosphates (OPs) and binapacryl (strain BR), (2) multi-resistant to OPs and tetradifon (strain TR), and (3) susceptible to OPs, binapacryl and tetradifon (strain EM). By crosses of EM females and BR males, and of EM females and TR males, and by repeated backcrossing with EM males under selection pressure of the acaricides concerned, two strains were produced in which the specific genes for resistance to binapacryl and tetradifon were isolated in the genome of the EM strain. Reciprocal crosses of the resulting backcrossed strains with EM, assays on the F1 eggs and the F2 haploid eggs, confirmed the presence of single major genes for resistance to binapacryl and tetradifon, and the absence of maternal effects. The expression of gene BR was incompletely dominant in response to binapacryl, whilst gene TR was effectively dominant in response to tetradifon. Eggs of the field-derived strain BR showed c. 50× resistance to binapacryl, 8–19 × to dinobuton, but only c. 2× to dinocap and dinoseb. The backcrossed strain was less resistant (26 ×) to binapacryl implying that resistance in strain BR was augmented by the effects of minor genes. Resistance to tetradifon (150–200 × on eggs) involved cross-resistance to tetrasul (23 ×) and to chlorfensulphide (27×); resistance in the field-derived strain appeared to depend on the major gene alone. Resistance mechanisms were highly specific in their effects, so that there was no cross-resistance to unrelated acaricides, including amitraz, dicofol, cyhexatin, formetanate, chlordimeform, and quinomethionate. Fecundity of strain BR was 44% less than that of EM; laboratory and field evidence suggest that multi-resistant mites tend to be less fit than susceptible mites. The practical implications are discussed. 相似文献
9.
Inheritance and Gene Mapping of Resistance to Soybean Mosaic Virus Strain SC14 in Soybean 总被引:4,自引:0,他引:4
Hai-Chao Li Hai-Jian Zhi Jun-Yi Gai Dong-Quan Guo Yan-Wei Wang Kai Li Li Bai Hua Yang 《植物学报(英文版)》2006,48(12):1466-1472
Soybean mosaic virus (SMV) is one of the most broadly distributed diseases worldwide. It causes severe yield loss and seed quality deficiency in soybean (Glycine max (L.) Merr.). SMV Strain SC14 isolated from Shanxi Province, China, was a newly identified virulent strain and can infect Kefeng No. 1, a source with wide spectrum resistance. In the present study, soybean accessions, PI96983, Qihuang No. 1 and Qihuang No. 22 were identified to be resistant (R) and Nannong 1138‐2, Pixianchadou susceptible (S) to SC14. Segregation analysis of PI96983 x Nannong 1138‐2 indicated that a single dominant gene (designated as RSC14) controlled the resistance to SC14 at both V2 and R1 developmental stages. The same results were obtained for the crosses of Qihuang No. 1 × Nannong 1138‐2 and Qihuang No. 22 × Nannong 1138‐2 as in PI96983 × Nannong 1138‐2 at V2 stage, but at R1 stage, the F1 performed as necrosis (a susceptible symptom other than mosaic), F2 segregated in a ratio of 1R:2N:1S, and the progenies of necrotic (N) F2 individuals segregated also in R, N and S. It indicated that a single gene (designated as RSC14Q, to be different from that of PI96983) controlled the resistance to SC14, its dominance was the same as in PI96983 × Nannong 1138‐2 (without symptoms) at V2 stage and not the same at R1 stage. The tightly linked co‐dominant simple sequence repeat (SSR) marker Satt334 indicated that all the heterozygous bands were completely corresponding to the necrotic F2 individuals, or all the necrotic F2 individuals were heterozygotes. It was inferred that necrosis might be due to the interaction among SMV strains, resistance genes, genetic background of the resistance genes, and plant development stage. Furthermore, the bulked segregant analysis (BSA) of SSR markers was conducted to map the resistance genes. In F2of PI96983 × Nannong 1138‐2, five SSR markers, Sat_297, Sat_234, Sat_154, Sct_033 and Sat_120, were found closely linked to RSC14, with genetic distances of 14.5 cM, 11.3cM, 4.3cM,3.2cM and 6cM, respectively. In F2 of Qihuang No. 1 × Nannong 1138‐2, three SSR markers, Sat_234, Satt334 and Sct_033, tightly linked to RSC14Q with genetic distances of 7.2 cM, 1.4 cM and 2.8 cM, respectively. Based on the integrated joint map by Cregan et al. (1999), both RScMand RSC14Q were located between Sat_234 and Sct_033 on linkage with group F of soybean, with their distances from Sct_033 at the same side being 3.2 cM and 2.8 cM, respectively. Therefore, RSC14and RSC14Q might be on a same locus. The obtained information provides a basic knowledge for marker‐assisted selection of the resistance gene in soybean breeding programs and fine mapping and map‐based cloning of the resistance gene. (Managing editor: Li‐Hui Zhao) 相似文献
10.
C. M. Kreike A. A. Kok-Westeneng J. H. Vinke W. J. Stiekema 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(3-4):463-470
A backcross population, derived from the cross (S. tuberosumxS. spegazzinii)xS. tuberosum was used to map QTLs involved in nematode resistance, tuber yield and root development. Complete linkage maps were available for the interspecific hybrid parent as well as the S. tuberosum parent, and interval mapping for all traits was performed for both. Additionally, the intra- and inter-locus interactions of the QTLs were examined. The Gro1.2 locus, involved in resistance to G. rostochiensis pathotype Ro1, that was previously mapped in the S. tuberosumxS. spegazzinii F1 population, was located more precisely on chromosome 10. A new resistance locus, Gro1.4, also conferring resistance to G. rostochiensis pathotype Ro1, was found on chromosome 3. Different alleles of this locus originating from both parents contributed to the resistant phenotype, indicating multiallelism at this locus. No interlocus interactions were observed between these two resistance loci. For resistance to G. pallida no QTLs were detected. One minor QTL involved in tuber yield was located on chromosome 4. Two QTLs involved in root development and having large effects were mapped on chromosomes 2 and 6 and an epistatic interaction was found between these two loci. 相似文献
11.
Janet Seager Danciger Michael Danciger Steven Nusinowitz Tammy Rickabaugh Debora B. Farber 《Mammalian genome》1999,10(7):657-661
The rd3 retinal degeneration gene was previously mapped 10 ± 2.5 cM distal to Akp1 on mouse Chromosome (Chr) 1 (Chang et al., 1993), a region that may be homologous to the locus of the human USH2A gene, which
carries mutations responsible for Usher IIa retinal degeneration/hearing loss syndrome. An intercross from an Rb(11,13)4Bnr(rd3/rd3) × C57BL/6J mating was set up, 428 F2 meioses were analyzed, and the rd3 gene was placed between the markers D1MIT292/D1MIT209 and D1MIT510, a distance of 1.40 ± 0.57 cM. These flanking markers and the mouse ortholog of USH2A (Mush2a) were mapped in the T31 mouse radiation hybrid (RH) panel, with the result that D1MIT292/D1MIT209 and D1MIT510 were 7.9 cR3000 apart (∼800 kb), and Mush2a was > 30 cR3000 proximal to the pair, excluding it from the rd3 locus. A contig spanning the rd3 locus and consisting of 2 YACs and one BAC was generated, and Mush2a was absent from it, confirming its exclusion from the locus. Comparison of adjacent marker pairs in the Whitehead genetic
map and our genetic map showed some discrepancies in order of markers and genetic distances. Comparison of our genetic map
and the RH map showed some highly skewed relationships between genetic and physical distances.
Received: 4 January 1999 / Accepted: 26 February 1999 相似文献
12.
Karen A. Gould Jyotsna Pandey Cynthia M. Lachel Clare R. Murrin Lisa A. Flood Karen L. Pennington Beverly S. Schaffer Martin Tochacek Rodney D. McComb Jane L. Meza Douglas L. Wendell James D. Shull 《Mammalian genome》2005,16(11):854-864
In certain rat strains, chronic estrogen administration can lead to pyometritis, an inflammation of the uterus accompanied
by infection and the accumulation of intraluminal pus. In this article, we report that the Brown Norway (BN) rat is highly
susceptible to pyometritis induced by 17β-estradiol (E2). The susceptibility of the BN rat to E2-induced pyometritis appears
to segregate as a recessive trait in crosses to the resistant August × Copenhagen Irish (ACI) strain. In a (BN × ACI)F2 population, we find strong evidence for a major genetic determinant of susceptibility to E2-induced pyometritis on rat chromosome
5 (RNO5). Our data are most consistent with a model in which the BN allele of this locus, designated Eutr1 (Estrogen-induced uterine response 1), acts in an incompletely dominant manner to control E2-induced pyometritis. Furthermore, we have confirmed the
contribution of Eutr1 to E2-induced uterine pyometritis using an RNO5 congenic rat strain. In addition to Eutr1, we obtained evidence suggestive of linkage for five additional loci on RNO2, 4, 11, 17, and X that control susceptibility
to E2-induced pyometritis in the (BN × ACI)F2 population. 相似文献
13.
Giacomo Manenti Manuela Gariboldi Antonio Fiorino Antonio I. Zedda Marco A. Pierotti Tommaso A. Dragani 《Mammalian genome》1997,8(11):801-804
Inherited predisposition to lung cancer is a phenotypic trait shared by different mouse inbred strains that show either a
high or an intermediate predisposition. Other strains are instead genetically resistant. The Pas1 locus is the major determinant of lung cancer predisposition in the A/J strain (Gariboldi et al. 1993). To define the determinants
of susceptibility to lung tumorigenesis in the highly susceptible SWR/J and in the intermediately susceptible BALB/c mice,
we analyzed (BALB/c × SWR/J)F2 and (BALB/c × C3H/He)F2 crosses by genetic linkage experiments. The present results provide unequivocal evidence that the same Pas1/+ allele that leads to lung cancer predisposition is shared by A/J, SWR/J, and BALB/c strains. The intermediate susceptibility
of the BALB/c strain would result by interaction of Pas1 locus with lung cancer resistance loci.
Received: 18 April 1997 / Accepted: 15 June 1997 相似文献
14.
Molly Sharlach Douglas Dahlbeck Lily Liu Joshua Chiu José M. Jiménez-Gómez Seisuke Kimura Daniel Koenig Julin N. Maloof Neelima Sinha Gerald V. Minsavage Jeffrey B. Jones Robert E. Stall Brian J. Staskawicz 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(3):601-609
The RXopJ4 resistance locus from the wild accession Solanum pennellii (Sp) LA716 confers resistance to bacterial spot disease of tomato (S. lycopersicum, Sl) caused by Xanthomonas perforans (Xp). RXopJ4 resistance depends on recognition of the pathogen type III effector protein XopJ4. We used a collection of Sp introgression lines (ILs) to narrow the RXopJ4 locus to a 4.2-Mb segment on the long arm of chromosome 6, encompassed by the ILs 6-2 and 6-2-2. We then adapted or developed a collection of 14 molecular markers to map on a segregating F2 population from a cross between the susceptible parent Sl FL8000 and the resistant parent RXopJ4 8000 OC7. In the F2 population, a 190-kb segment between the markers J350 and J352 cosegregated with resistance. This fine mapping will enable both the identification of candidate genes and the detection of resistant plants using cosegregating markers. The RXopJ4 resistance gene(s), in combination with other recently characterized genes and a quantitative trait locus (QTL) for bacterial spot disease resistance, will likely be an effective tool for the development of durable resistance in cultivated tomato. 相似文献
15.
Genetic analysis and fine mapping of a resistance gene against brown planthopper (BPH) biotype 2 in rice was performed using
two F2 populations derived from two crosses between a resistant indica cultivar (cv.), AS20-1, and two susceptible japonica cvs., Aichi Asahi and Lijiangxintuanheigu. Insect resistance was evaluated using F1 plants and the two F2 populations. The results showed that a single recessive gene, tentatively designated as bph19(t), conditioned the resistance in AS20-1. A linkage analysis, mainly employing microsatellite markers, was carried out in
the two F2 populations through bulked segregant analysis and recessive class analysis (RCA), in combination with bioinformatics analysis
(BIA). The resistance gene locus bph19(t) was finely mapped to a region of about 1.0 cM on the short arm of chromosome 3, flanked by markers RM6308 and RM3134,
where one known marker RM1022, and four new markers, b1, b2, b3 and b4, developed in the present study were co-segregating
with the locus. To physically map this locus, the bph19(t)-linked markers were landed on bacterial artificial chromosome or P1 artificial chromosome clones of the reference cv.,
Nipponbare, released by the International Rice Genome Sequencing Project. Sequence information of these clones was used to
construct a physical map of the bph19(t) locus, in silico, by BIA. The bph19(t) locus was physically defined to an interval of about 60 kb. The detailed genetic and physical maps of the bph19(t) locus will facilitate marker-assisted gene pyramiding and cloning. 相似文献
16.
Maureen K. Purcell Jian-Long Mu David C. Higgins Ramu Elango Harry Whitmore Stephen Harris Beverly Paigen 《Mammalian genome》2001,12(7):495-500
Ath6 is a novel quantitative trait locus associated with differences in susceptibility to atherosclerosis between C57BL/6J (B6)
and C57BLKS/J (BKS) inbred mouse strains. Combining data from an intercross and a backcross (1593 meioses) between mice from
B6 and BKS strains and from The Jackson Laboratory interspecific backcross panels, (C57BL/6J ×Mus spretus) F1× C57BL/6J and (C57BL/6J × SPRET/Ei) F1× SPRET/Ei, we constructed a consensus genetic map and narrowed Ath6 to a 1.07 ± 0.26 cM interval between the anonymous DNA marker D12Pgn4 and the gene Nmyc1. This region is near the proximal end of murine Chromosome (Chr) 12, which is homologous to the human chromosomal region 2p24-p25.
Marker order in the Ath6 region was concordant among the two crosses and The Jackson Laboratory interspecific backcross panels. This high resolution
map rules out candidate genes encoding apolipoprotein B, syndecan 1, and Adam17. The two Ath6 crosses have a combined potential resolution of 0.06 cM.
Received: 12 September 2000 / Accepted: 22 February 2001 相似文献
17.
J. C. Veremis A. W. van Heusden P. A. Roberts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(2):274-280
The root-knot nematode heat-stable resistance locus from L. peruvianum LA2157 was mapped on chromosome 6. All wild tomato LA2157 entries and the LA2157 S1 progeny tested were resistant to Mi-avirulent Meloidogyne spp. isolates at 32°C, indicating that the self-compatible accession is homozygous for heat-stable nematode resistance. The
novel resistance locus was mapped on a RFLP linkage map; this map was based on a segregating F2 population obtained from the interspecific F1 between L. esculentum cv ‘Solentos’ and L. peruvianum LA2157. The inheritance of the heat-stable resistance was evaluated in 100 F3 lines derived from one F1 interspecific hybrid. The genotype of the resistance locus of the individual F2 plants was based on the phenotypic classification of their F3 lines, and the data were used to map the resistance locus on the arm of chromosome 6 with the closest linkage to TG178. The
position of the novel heat-stable resistance of LA2157 was localized in the resistance genes’ cluster close to the location
of gene Mi-1. Cuttings of the F3 lines expressed resistance to Mi-1-avirulent M. incognita and M. javanica biotypes at 25°C and at 32°C (a temperature at which Mi-1 resistance is not expressed). There was no difference in the segregating population for expression of heat-unstable resistance
and heat-stable resistance to Mi-1-avirulent Meloidogyne spp. However, LA2157 and cuttings of the above F3 lines were susceptible to a Mi-1-virulent M. incognita isolate at 30°C and to a M. hapla isolate at 25°C.
Received: 6 July 1998 / Accepted: 28 July 1998 相似文献
18.
Sibylle Stoeckli Karsten Mody Cesare Gessler Andrea Patocchi Mauro Jermini Silvia Dorn 《Tree Genetics & Genomes》2008,4(4):833-847
The rosy apple aphid (Dysaphis plantaginea), the leaf-curling aphid (Dysaphis cf. devecta) and the green apple aphid (Aphis pomi) are widespread pest insects that reduce growth of leaves, fruits and shoots in apple (Malus × domestica). Aphid control in apple orchards is generally achieved by insecticides, but alternative management options like growing
resistant cultivars are needed for a more sustainable integrated pest management (IPM). A linkage map available for a segregating
F1-cross of the apple cultivars ‘Fiesta’ and ‘Discovery’ was used to investigate the genetic basis of resistance to aphids.
Aphid infestation and plant growth characteristics were repeatedly assessed for the same 160 apple genotypes in three different
environments and 2 consecutive years. We identified amplified fragment length polymorphism (AFLP) markers linked to quantitative
trait loci (QTLs) for resistance to D. plantaginea (‘Fiesta’ linkage group 17, locus 57.7, marker E33M35–0269; heritability: 28.3%), and to D. cf. devecta (‘Fiesta’ linkage group 7, locus 4.5, marker E32M39–0195; heritability: 50.2%). Interactions between aphid species, differences
in climatic conditions and the spatial distribution of aphid infestation were identified as possible factors impeding the
detection of QTLs. A pedigree analysis of simple sequence repeat (SSR) marker alleles closely associated with the QTL markers
revealed the presence of the alleles in other apple cultivars with reported aphid resistance (‘Wagener’, ‘Cox’s Orange Pippin’),
highlighting the genetic basis and also the potential for gene pyramiding of aphid resistance in apple. Finally, significant
QTLs for shoot length and stem diameter were identified, while there was no relationship between aphid resistance and plant
trait QTLs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
19.
An autosomal recessive deafness mutant was discovered in our colony of Zucker (ZUC) rats. These mutants behave like shaker-waltzer
deafness mutants, and their inner ear pathology classifies them among neuroepithelial degeneration type of deafness mutants.
To determine whether this rat deafness mutation (−) defines a unique locus or one that has been previously described, we mapped
its chromosomal location. F2 progeny of (Pbrc:ZUC × BN/Crl) A/a B/b
H/h+/− F1 rats were scored for coat color and behavioral phenotypes. Segregation analysis indicated that the deafness locus might be
loosely linked with B on rat Chromosome (Chr) 5 (RNO5). Therefore, 40 −/− rats were scored for BN and ZUC alleles at four additional loci, D5Mit11, D5Mit13, Oprd1, and Gnb1, known to map to RNO5 or its homolog, mouse Chr 4 (MMU4). Linkage analysis established the gene order (cM distance) as D5Mit11–(19.3)–B–(17.9)–D5Mit13–(19.2)–Oprd1–(21.5) − (1.2) Gnb1, placing the deafness locus on distal RNO5. The position of the deafness locus on RNO5 is similar to that ofjerker (je) on MMU4; the phenotypes and patterns of inheritance of the deafness mutation and je are also similar. It seems likely that the mutation affects the rat homolog of je. The rat deafness locus should, therefore, be named jerker and assigned the gene symbol Je.
Received: 13 June 1995 / Accepted: 4 January 1996 相似文献
20.
Localization of the rice stripe disease resistance gene, Stv-bi, by graphical genotyping and linkage analyses with molecular markers 总被引:4,自引:0,他引:4
Y. Hayano-Saito T. Tsuji K. Fujii K. Saito M. Iwasaki A. Saito 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(8):1044-1049
We used graphical genotyping and linkage analyses with molecular markers to determine the chromosomal location of the rice
stripe disease resistance gene, Stv-b
i
. The stripe resistance gene from the indica rice (Oryza sativa) cv ‘Modan’ was introgressed into several Japanese rice varieties. We found 4 RFLP markers in ‘Modan’, five susceptible parental
rice varieties (‘Norin No. 8’, ‘Sachihikari’, ‘Kanto No. 98’, ‘Hokuriku No.103’ and ‘Koganebare’) and four resistant progeny
varieties (‘St. No. 1’, ‘Aichi No. 6’, ‘Aoisora’ and ‘Asanohikari’). Graphical genotyping of the resistant progeny revealed
a chromosomal segment ascribable to ‘Modan’ and associated with stripe resistance. The chromosomal segment from ‘Modan’ was
located at 35.85 cM on chromosome 11. Linkage analysis using 120 F2 individuals from a cross between ‘Koshihikari’ (susceptible) and ‘Asanohikari’ (resistant) revealed another 8 RFLP markers
in the same chromosome. We performed a bioassay for rice stripe resistance in F3 lines of the F2 individuals using infective small brown planthoppers and identified an 1.8-cM segment harboring the rice stripe disease resistance
gene, Stv-b
i
, between XNpb220 and XNpb257/ XNpb254. Furthermore, Stv-b
i
was linked by 0.0 cM to a RFLP marker, ST10, which was developed on the basis of the results of RAPD analysis. These DNA
markers near the Stv-b
i
locus may be useful in marker-assisted selection and map-based cloning of the Stv-b
i
gene.
Received: 26 September 1997 / Accepted: 4 November 1997 相似文献