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1.
Aphis fabae was reared on synthetic diets omitting individual amino acids and amino acid groups. Methionine and histidine deletions induced apterae formation and prevented the aphids from reaching the adult stage, whereas arginine, leucine, lysine, and proline deletions induced alatae formation. The omission of alanine, cysteine, phenylalanine, proline, serine, and tyrosine reduced the size attained by adult alatae and together with methionine and histidine, these amino acids are considered to be essential for normal growth by A. fabae. A delay in parturition occurred in aphids reared on certain deficient diets and there is an association between the size attained by alatae and the delay in parturition. 相似文献
2.
In hydrolysates of the eastern subterranean termite, Reticulitermes flavipes, the most abundant protein amino acids (μmoles) were glycine, alanine, and glutamic acid; the least abundant were methionine and histidine. Sawdust from both sound and Lenzites trabea-decayed sapwood blocks of sugar maple, loblolly pine, and slash pine was force-fed to termites. A diet of decayed rather than sound wood had little effect on protein amino acid composition of the termites; glycine content varied the most. In contrast, diet affected the free amino acid composition. Except for glutamic acid, the major protein amino acids of the termites were not the predominant free amino acids. Tyrosine and histidine were relatively more abundant as free than as protein amino acids. Greatest differences in protein amino acid compositions of sound and decayed wood were in contents of glycine, leucine, lysine, and arginine. 相似文献
3.
Kevin J. Vogel Nancy A. Moran 《Proceedings. Biological sciences / The Royal Society》2011,278(1702):115-121
The nutritional symbiosis between aphids and their obligate symbiont, Buchnera aphidicola, is often characterized as a highly functional partnership in which the symbiont provides the host with essential nutrients. Despite this, some aphid lineages exhibit dietary requirements for nutrients typically synthesized by Buchnera, suggesting that some aspect of the symbiosis is disrupted. To examine this phenomenon in the pea aphid, Acyrthosiphon pisum, populations were assayed using defined artificial diet to determine dietary requirements for essential amino acids (EAAs). Six clones exhibiting dependence on EAAs in their diet were investigated further. In one aphid clone, a mutation in a Buchnera amino acid biosynthesis gene could account for the clone''s requirement for dietary arginine. Analysis of aphid F1 hybrids allowed separation of effects of the host and symbiont genomes, and revealed that both affect the requirement for dietary EAAs in the clones tested. Amino acid requirements were minimally affected by secondary symbiont infection. Our results indicate that variation among pea aphids in dependence on dietary amino acids can result from Buchnera mutation as well as variation in the host genotype. 相似文献
4.
The failure of a nutritionally balanced diet to ameliorate the impact of symbiont disruption in the pea aphid Acyrthosiphon pisum (Harris) was investigated using two approaches. The assimilation of dietary nutrients by aphids was investigated using chemically-defined diets containing 3 H-labelled inulin and 14C-labelled sucrose or amino acids. Symbiotic aphids (i.e., aphids containing their bacteria) had a high sucrose demand and assimilated 72% of sucrose ingested in the diet, whereas the assimilation of sucrose by aposymbiotic aphids (in which the bacteria had been disrupted), was significantly reduced to 47%. The assimilation of individual dietary amino acids by symbiotic aphids varied between 61 and 92%, and there was no impact on the feeding or assimilation rate when the aphids were fed a phloem sap-like diet containing a reduced amount of essential amino acids. Consequently, the absolute amount of each essential amino acid assimilated by symbiotic aphids feeding on a phloem sap-like diet was reduced by 36–59%. Aposymbiotic aphids consistently assimilated a lower proportion of ingested amino acids, and lysine in particular was poorly assimilated from the diet. In a second experiment, the allocation of free amino acids in the haemocoel to aphid embryos was investigated following microinjection of 14C-labelled amino acids. After 2 h, radiolabel could be detected at varying levels from the embryo complement of both symbiotic and aposymbiotic aphids, indicating rapid but selective uptake by the embryos. The essential amino acids phenylalanine and lysine were incorporated into the protein fraction of embryo tissues, but the rate of incorporation per unit biomass was approximately 4-fold higher in the embryos of aposymbiotic aphids, possibly reflecting increased demand due to the lack of amino acid provisioning from the symbiotic bacteria. 相似文献
5.
Feeding experiments with mixtures of purified amino acids show that arginine, leucine, phenylalanine, and valine are required for good growth of weanling pigs. The pig resembles the rat in its ability to synthesize part, but not all of the arginine required. It is now possible to tentatively classify the known amino acids with respect to major growth effects in weanling pigs. The amino acids which must be present in the diet for good growth are arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine. The remaining amino acids may be tentatively classed as dispensable. 相似文献
6.
Summary Rust infected wheat plants were incubated with different 14C-labelled amino acids. Uredospores that were formed during the incubation contained 14C-activity. By analysis of these spores it was investigated whether the parasitic mycelium of Puccinia graminis takes amino acids from the host. It could be demonstrated that the applicated amino acids were taken up directly from the wheat leaf. The carbon sceletons of applicated lysine and arginine showed only little randomization of 14C-activity. Glutamic acid, alanine and glycine isolated from uredospore protein showed a very strong alteration of the original label. The pools of free amino acids in the host and the parasite are in isotope equilibrium. This demonstrates, that synthesis of amino acids in the mycelium is quantitatively not important. By following the kinetics of incorporation of an amino acid it could be demonstrated that the amino acids enter the parasite as free amino acids and not in the form of proteins. 相似文献
7.
The intraerythrocytic malaria parasite, Plasmodium falciparum, derives amino acids from the digestion of host cell haemoglobin. However, it also takes up amino acids from the extracellular medium. Isoleucine is absent from adult human haemoglobin and an exogenous source of isoleucine is essential for parasite growth. An extracellular source of methionine is also important for the normal growth of at least some parasite strains. In this study we have characterised the uptake of methionine by P. falciparum-infected human erythrocytes, and by parasites functionally isolated from their host cells by saponin-permeabilization of the erythrocyte membrane. Infected erythrocytes take up methionine much faster than uninfected erythrocytes, with the increase attributable to the flux of this amino acid via the New Permeability Pathways induced by the parasite in the erythrocyte membrane. Having entered the infected cell, methionine is taken up by the intracellular parasite via a saturable, temperature-dependent process that is independent of ATP, Na+ and H+. Substrate competition studies, and comparison of the transport of methionine with that of isoleucine and leucine, yielded results consistent with the hypothesis that the parasite has at its surface one or more transporters which mediate the flux into and out of the parasite of a broad range of neutral amino acids. These transporters function most efficiently when exchanging one neutral amino acid for another, thus providing a mechanism whereby the parasite is able to import important exogenous amino acids in exchange for surplus neutral amino acids liberated from the digestion of host cell haemoglobin. 相似文献
8.
The majority (10 of 17) of amino acids tested entered the mature duck erythrocyte by a saturable, non-uphill transport system, whereas for the erythrocyte-free malarial parasite, Plasmodium lophurae, the converse was true: most amino acids entered the parasite by simple diffusion. Only five amino acids (glutamic and aspartic acids, cysteine, lysine, arginine) showed mediated entry into P. lophurae. The pattern of mediated amino acid transport into the duck erythrocyte was altered upon infection, e.g., either entry was by diffusion or there was a reduced affinity for the amino acid. Transport characteristics similar to those found in the malaria-infected erythrocyte were produced by treating normal duck red cells with a cell-free extract of malaria-infected erythrocytes and quinine (a depressor of red cell ATP). It is suggested that depletion of host cell ATP as well as elaboration of as yet unidentified substances by the parasite promote the changes in permeability seen in the malaria-infected cell. 相似文献
9.
Stephen J. Fairweather Esther Rajendran Martin Blume Kiran Javed Birte Steinhfel Malcolm J. McConville Kiaran Kirk Stefan Brer Giel G. van Dooren 《PLoS pathogens》2021,17(8)
Intracellular parasites of the phylum Apicomplexa are dependent on the scavenging of essential amino acids from their hosts. We previously identified a large family of apicomplexan-specific plasma membrane-localized amino acid transporters, the ApiATs, and showed that the Toxoplasma gondii transporter TgApiAT1 functions in the selective uptake of arginine. TgApiAT1 is essential for parasite virulence, but dispensable for parasite growth in medium containing high concentrations of arginine, indicating the presence of at least one other arginine transporter. Here we identify TgApiAT6-1 as the second arginine transporter. Using a combination of parasite assays and heterologous characterisation of TgApiAT6-1 in Xenopus laevis oocytes, we demonstrate that TgApiAT6-1 is a general cationic amino acid transporter that mediates both the high-affinity uptake of lysine and the low-affinity uptake of arginine. TgApiAT6-1 is the primary lysine transporter in the disease-causing tachyzoite stage of T. gondii and is essential for parasite proliferation. We demonstrate that the uptake of cationic amino acids by TgApiAT6-1 is ‘trans-stimulated’ by cationic and neutral amino acids and is likely promoted by an inwardly negative membrane potential. These findings demonstrate that T. gondii has evolved overlapping transport mechanisms for the uptake of essential cationic amino acids, and we draw together our findings into a comprehensive model that highlights the finely-tuned, regulated processes that mediate cationic amino acid scavenging by these intracellular parasites. 相似文献
10.
Sequestration and metabolism of host cell arginine by the intraerythrocytic malaria parasite Plasmodium falciparum
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Human erythrocytes have an active nitric oxide synthase, which converts arginine into citrulline and nitric oxide (NO). NO serves several important functions, including the maintenance of normal erythrocyte deformability, thereby ensuring efficient passage of the red blood cell through narrow microcapillaries. Here, we show that following invasion by the malaria parasite Plasmodium falciparum the arginine pool in the host erythrocyte compartment is sequestered and metabolized by the parasite. Arginine from the extracellular medium enters the infected cell via endogenous host cell transporters and is taken up by the intracellular parasite by a high‐affinity cationic amino acid transporter at the parasite surface. Within the parasite arginine is metabolized into citrulline and ornithine. The uptake and metabolism of arginine by the parasite deprive the erythrocyte of the substrate required for NO production and may contribute to the decreased deformability of infected erythrocytes. 相似文献
11.
Oscar H. Will III 《Current microbiology》1983,9(3):163-167
Cytochrome c was extracted and purified from nine species of the genusUstilago, representing five pathogen for monocotyledonous and four for dicotyledonous host species. The amino acid compositions of acid hydrolysates of cytochrome c from these species were compared and divergence values were calculated for all pairs of species. Aspartic acid, serine, glutamic acid, proline, glycine, alanine, valine, leucine, phenylalanine, lysine, and arginine were the most variable amino acids for both dicot and monocot pathogens. Differences in lysine, glutamic acid, alanine, and histidine distinguished most monocot and dicot pathogens. The dicotyledonous pathogens had fewer glutamic acid and additional alanine and histidine residues. Divergence values for cytochrome c were generally highest between species of dicotyledonous and monocotyledonous hosts and lowest for species within the monocotyledonous group. Pairs of species in the dicotyledonous group gave values only slightly lower than those for pairs with species from different groups. 相似文献
12.
The effect of ten amino acids as the sole nitrogen source for the growth of Cladosporium herbarum (Link.) Fr. and Trichothecium roseum (Bull.) Link. was studied in order to clarify the fungus-host plant relationship. Special attention was paid to some rare non-protein amino acids of legumes. The best nitrogen sources for both fungi were γ-aminobutyric acid, arginine, serine and proline. Cladosporium could use homoarginine and canavanine, but these two amino acids were not used by Trichothecium when each was given as the only nitrogen source. Both fungi utilized ornithine, homoserine and a,γ-diaminobutyric acid to a limited extent. Pipecolic acid was not growth promoting. The growth-retarding effects of rare non-protein amino acids (homoarginine, canavanine, a,γ-diaminobutyric acid and pipecolic acid) were usually reversed by higher concentrations of their normal analogues. It is possible that rare non-protein amino acids may slightly protect the host plant against fungal infections, but there are clear differences between fungi in their reaction to non-protein amino acids. 相似文献
13.
Michihiro Sugahara Takao Morimoto Takaaki Kobayashi Shujiro Ariyoshi 《Bioscience, biotechnology, and biochemistry》2013,77(1):77-84
The nutritional values of 16 D-amino acids in chick growth were studied on the purified diets containing crystalline amino acids as a sole source of nitrogen. Growth rate, feed consumption and nitrogen retention were measured. The nutritional values of D-amino acids were studied by comparing individually with the control groups fed on the diet containing all L-amino acids and negative control groups fed with the diet omitted the corresponding L-isomer. The following results were obtained. Essential amino acids: 1. Equal or almost equal nutritional value to the corresponding L-isomer; methionine, phenylalanine, leucine, proline. 2. Half nutritional value compared with L-isomer; valine. 3. Small nutritional value compared with L-isomer; tryptophan, isoleucine, histidine. 4. No nutritional value; lysine, threonine, arginine. Non-essential amino acid: 1. Equal or almost equal nutritional value to the corresponding L-isomer; serine, tyrosine, cystine. 2. There is a possibility that it has a slight growth retardation effect; alanine. 3. The growth retardation effect was found; aspartic acid. 相似文献
14.
Twelve amino acids and amides at 0·1 to 0·75 or 1·0% in 35% sucrose solution were individually tested for their rôle in phagostimulation, growth, and survival in Acyrthosiphon pisum. Leucine and phenylalanine were phagostimulatory at all concentrations tested, tryptophan and valine at 0·1, 0·2, and 0·5%, and threonine at 0·1% only. Methionine was reported earlier by us to be phagostimulatory at 0·05 to 0·5%. Histidine and isoleucine had no effect, whereas arginine and lysine HCl reduced uptake when compared to sucrose alone. The non-essential amino acids, canavanine sulphate and glutamine, reduced uptake at all concentrations, whereas homoserine was phagostimulatory at 0·1 and 0·75%.Arginine, canavanine sulphate, glutamine, histidine, homoserine, isoleucine, leucine, and valine increased weight and prolonged survival, whereas lysine HCl, phenylalanine, threonine, and tryptophan neither promoted growth nor increased survival. Radioactive leucine (14C(U)) was incorporated into the protein fraction of the larval body and exuviae indicating that it took part in protein synthesis. This seems to be the first report in insects where peptide or protein synthesis occurred from single amino acids in sucrose. 相似文献
15.
《Animal : an international journal of animal bioscience》2016,10(7):1148-1154
Apparent ileal digestibility (AID) of energy, dry matter (DM), nitrogen (N) and amino acids and standardized ileal digestibility (SID) of amino acids in low quality soybean meals with different CP concentration (SBM 44% CP and SBM 48% CP) with or without 400 U β-mannanase/kg supplementation were evaluated in 20 cannulated barrows ((Landrace×Yorkshire)×Duroc) with an average BW of 25.08±3.42 kg. A N-free diet was used to determine basal endogenous losses of amino acids. The supplementation of β-mannanase improved (P<0.05) AID of DM compared with non-supplemented diet. However, enzyme supplementation did not improve (P>0.05) AID of N and energy. The type of SBM (SBM 44% CP v. SBM 48% CP) had no effect on AID of DM, N and energy. β-mannanase improved (P<0.05) AID of sum of essential amino acids, arginine, histidine, lysine, valine and glycine. The SID of lysine was higher (P<0.05) in enzyme supplemented than in non-supplemented diets. Larger AID and SID of threonine and proline (P<0.05) were observed in SBM 48% CP than in SBM 44% CP. In conclusion, the supplementation of enzyme improved AID of arginine, histidine, lysine, valine and glycine, but it did not cause marked difference in SID of these amino acids except for lysine. The low nutrient digestibility of the SBM sources used in the present experiment might have favoured the positive effect of β-mannanase supplementation. 相似文献
16.
The general control of amino acid biosynthesis was investigated in Candida spec. EH 15/D, using single and double mutant auxotrophic strains and prototrophic revertants starved for their required amino acids. These experiments show that starvation for lysine, histidine, arginine, leucine, threonine, proline, serine, methionine, homoserine, asparagine, glutamic acid or aspartic acid can result in derepression of enzymes. A correlation was found between the degree of derepression, growth of strains, and concentration of required amino acids. The amino acids pool pattern of mutants and revertants is different from that in the wild type strain. 相似文献
17.
Aphid-host plant interactions: does aphid honeydew exactly reflect the host plant amino acid composition? 总被引:1,自引:0,他引:1
Pascal D. Leroy Bernard Wathelet Ahmed Sabri Frédéric Francis François J. Verheggen Quentin Capella Philippe Thonart Eric Haubruge 《Arthropod-Plant Interactions》2011,5(3):193-199
Plants provide aphids with unbalanced and low concentrations of amino acids. Likely, intracellular symbionts improve the aphid
nutrition by participating to the synthesis of essential amino acids. To compare the aphid amino acid uptakes from the host
plant and the aphids amino acid excretion into the honeydew, host plant exudates (phloem + xylem) from infested and uninfested
Vicia faba L. plants were compared to the honeydew produced by two aphid species (Acyrthosiphon pisum Harris and Megoura viciae Buckton) feeding on V. faba. Our results show that an aphid infestation modifies the amino acid composition of the infested broad bean plant since the
global concentration of amino acids significantly increased in the host plant in response to aphid infestations. Specifically,
the concentrations of the two amino acids glutamine and asparagine were strongly enhanced. The amino acid profiles from honeydews
were similar for the two aphid species, but the concentrations found in the honeydews were generally lower than those measured
in the exudates of infested plants (aphids uptakes). This work also highlights that aphids take large amounts of amino acids
from the host plant, especially glutamine and asparagine, which are converted into glutamic and aspartic acids but also into
other essential amino acids. The amino acid profiles differed between the host plant exudates and the aphid excretion product.
Finally, this study highlights that the pea aphid, a “specialist” for the V. faba host plant, induced more important modifications into the host plant amino acid composition than the “generalist” aphid M. viciae. 相似文献
18.
Abstract. Nutrient provisioning in aphids depends both on the composition of ingested phloem sap and on the biosynthetic capabilities of the aphid and its intracellular symbionts. Amino acid budgets for three aphid species, Rhopalosiphum padi (L.), Schizaphis graminum (Rondani) and Diuraphis noxia (Mordvilko), were compared on a single host plant species, wheat Triticum aestivum L. Ingestion of amino acids from phloem, elimination of amino acids in honeydew, and the content of amino acids in aphids tissue were measured. From these values, ingestion rates were estimated and compared to honeydew and to estimated composition of aphid proteins. Ingestion rate was lowest in D. noxia due to low growth rate and low honeydew production; intermediate in S. graminum due to higher growth rate and intermediate honeydew production; and highest in R. padi , which had the highest rates for both variables. Both D. noxia and S. graminum induced increases in the amino acid content of ingested phloem. These changes in phloem content, combined with differences in ingestion rates, resulted in large differences among aphids in estimated rates of ingestion of individual amino acids. In honeydew, most essential amino acids were found in low amounts compared with the amounts ingested, especially for methionine and lysine. A few amino acids (arginine, cystine, histidine and tryptophan) were more abundant in honeydew of some aphids, suggesting oversupply. Aphid species differed in the composition of free amino acids in tissue but showed very similar composition in protein, implying similar requirements among the aphids. In R. padi and D. noxia , most essential amino acids were ingested in amounts insufficient for growth, implying dependence on symbiont provisioning. In S. graminum , most amino acids were ingested in amounts apparently sufficient for growth. 相似文献
19.
Determinations were made of free amino acids in hemolymph collected from adult female Anopheles stephensi mosquitoes. The hemolymph first was fractionated by extraction and precipitation procedures, after which qualitative determinations of free amino acids were made by high voltage thin layer electrophoresis, and thin layer chromatography. Subsequent quantitative determinations were made with an automatic amino acid analyzer. The concentration of total free amino acids in the hemolymph rose 60--70% after the mosquito took a blood meal, and remained relatively constant thereafter. When mosquitoes took a blood meal infected with the rodent malaria parasite Plasmodium berghei, the rise in total free amino acids was only 15--25%. The chief differences that occurred with individual free amino acids was that infected mosquitoes had greater increases in arginine, greater decreases in valine and histidine, and a total loss of detectable methionine. 相似文献
20.
A comparison was made of the in vitro growth of the gut of Culex tarsalis in Grace's insect culture medium, supplemented with fetal bovine serum in the presence of dividing cells of Antheraea eucalypti, with a similar preparation of a gut infected with oocysts of the avian parasite, Plasmodium relictum. In the latter case, after 16 hr, significant decreases occurred in the concentration of arginine, asparagine, and glutamine combined, glutamic acid, glycine, histidine, lysine, proline, and serine. Lower and less marked decreased concentrations of alanine, β-alanine, cystine, isoleucine, leucine, methionine, ornithine, phenylalanine, threonine, tryptophane, tyrosine, and valine also took place. This indicated utilization of certain amino acids by the developing oocysts of P. relictum in the presence of metabolizing insect cells. 相似文献