共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
3.
4.
Osteopontin is a negative feedback regulator of nitric oxide synthesis in murine macrophages 总被引:21,自引:0,他引:21
Guo H Cai CQ Schroeder RA Kuo PC 《Journal of immunology (Baltimore, Md. : 1950)》2001,166(2):1079-1086
5.
6.
7.
8.
9.
R J Michelson M W Collard A J Ziemba J Persinger B Bartholomew J I Huggenvik 《The Journal of biological chemistry》1999,274(43):30510-30519
10.
Xinghua Lin Hong Yang Hongfeng Zhang LiChun Zhou ZhongMao Guo 《The Journal of biological chemistry》2013,288(21):14815-14823
11.
12.
13.
14.
Zhao W Wang L Zhang M Wang P Zhang L Yuan C Qi J Qiao Y Kuo PC Gao C 《Journal of immunology (Baltimore, Md. : 1950)》2011,186(5):3173-3179
Osteopontin (OPN) is expressed by various immune cells and modulates both innate and adaptive immune responses. However, the molecular mechanisms that control opn gene expression, especially at the chromatin level, remain largely unknown. We have previously demonstrated many specific cis- and trans-regulatory elements that determine the extent of endotoxin (LPS)-mediated induction of OPN synthesis in murine macrophages. In the present study, we confirm that NF-κB also plays an important role in the setting of LPS-stimulated OPN expression through binding to a distal regulatory element. Importantly, we demonstrate that LPS stimulates chromosomal loops in the OPN promoter between NF-κB binding site and AP-1 binding site using chromosome conformation capture technology. The crucial role of NF-κB and AP-1 in LPS-stimulated DNA looping was confirmed, as small interfering RNA knock-down of NF-κB p65 and AP-1 c-Jun exhibited decreased levels of DNA looping. Furthermore, we demonstrate that p300 can form a complex with NF-κB and AP-1 and is involved in DNA looping and LPS-induced OPN expression. Therefore, we have identified an essential mechanism to remodel the local chromatin structures and spatial conformations to regulate LPS-induced OPN expression. 相似文献
15.
16.
17.
18.
Hershko DD Robb BW Luo GJ Hungness ES Hasselgren PO 《Journal of cellular biochemistry》2003,90(3):627-640
19.