Influence of the shape of the cross section of a plasma-dielectric interface on the dispersion properties of high-frequency azimuthal surface modes

Theoretical study of the propagation of a packet of surface electromagnetic surface waves with a zero axial wavenumber in a circular-cross-section cylindrical metal waveguide within the frequency range that is higher than upper hybrid resonance is carried out. The waveguide is partially filled by plasma and immersed into axial magnetic field. The cross section of the plasma column is assumed to differ from circular shape. The effect of this shape on the dispersion properties of azimuthal surface modes is investigated by the method of successive approximations. The fields of the waves and their eigenfrequencies are determined up to terms of the second order in the deviation of the plasma cross section shape from the ring one. The correction to the eigenfrequency of azimuthal surface modes caused by this feature of the plasma column section is proved to increase with decreasing the external magnetic field and increasing the value of the dielectric constant of the dielectric, that separates the plasma from the metal wall of the waveguide. The spectral composition of the wave packet, in the form of which these modes propagate, is studied. The amplitudes of the satellite harmonics of these modes are found to increase with increasing the plasma density and decreasing the external magnetic field.     

Resonant effect of the noncircular shape of the plasma surface on the dispersion properties of extraordinary azimuthal surface modes in magnetoactive waveguides

A theoretical study is made of the resonant effect of the shape of the cross section of the plasma column on the propagation of a packet of extraordinary electromagnetic waves with a zero axial wavenumber in a circular-cross-section cylindrical metal waveguide in an axial magnetic field. The waveguide is assumed to be partially filled with a plasma. The effect of the noncircular shape of the plasma cross section on the dispersion properties of surface eigenmodes propagating strictly transverse to the external magnetic field is investigated by the method of successive approximations for the case in which the angular period of the wave perturbations is twice the ripple period of the interface between the plasma and the dielectric. In this resonant case, the fields and eigenfrequencies of the eigenmodes are determined to second order in the small parameter describing the rippling of the plasma-dielectric interface.     

Excitation of azimuthal surface modes by relativistic flows of electrons in the high-frequency range

Excitation of extraordinarily polarized azimuthal surface eigenwaves is shown to be possible in the frequency range above the upper hybrid resonance in waveguides with metal walls which are partially filled by cold magnetoactive plasma. Interaction of these waves with flows of electrons which rotate around the plasma column in the narrow gap separating the plasma from the wall of the waveguide is studied. Conditions of resonant interaction of the beam with the mentioned high-frequency azimuthal surface waves are shown by numerical methods to be reachable ones in the case of enough strong external magnetic fields without passing to the field of ultra-relativistic velocities of the beam.     

Excitation of high-frequency azimuthal surface waves by an annular electron beam in a waveguide with a noncircular interface of the plasma column

An initial stage of the interaction of an electron beam ring rotating along Larmor orbits in a gap between the plasma column and a circular metal chamber of a cylindrical waveguide with extraordinarily polarized electromagnetic waves of the surface type is studied. These waves propagate along the azimuthal angle across an axial magnetic field in the range above the upper hybrid frequency. Using numerical analysis of the dispersion relation, it is shown that by the aid of an appropriate choice of the shape of the plasmavacuum interface one can achieve a significant increasing of growth rates of the resonant beam instability of these waves.     

Effect of sand shape on evaporation from soil surface

Summary Loss of water through evaporation for different types of soils was examined. Soils were prepared from a clay soil and multiangular or platy sand in different proportions. The evaporation curves are of the type z=a+bx+cx² but with different slopes. Both soils had the same percentage of clay and sand but platy-sand soils behaved as if they had a finer texture.     

On the dispersion of geodesic acoustic modes

The problem of dispersion of geodesic acoustic modes is revisited with two different methods for the solution of the kinetic equation. The dispersive corrections to the mode frequency are calculated by including the m = 2 poloidal harmonics. Our obtained results agree with some earlier results but differ in various ways with other previous works. Limitations and advantages of different approaches are discussed.     

Effect of the lipid interface on the catalytic activity and spectroscopic properties of a fungal lipase

Lipase from the fungi Thermomyces (formerly Humicola) lanuginosa (TlL) is widely used in industry. This interfacial enzyme is inactive under aqueous conditions, but catalytic activation is induced on binding to a lipid-water interface. In order for protein engineering to design more efficient mutants of TlL for specific applications, it is important to characterize its interfacial catalysis. A complete analysis of steady-state kinetics for the hydrolysis of a soluble substrate by TlL has been developed using an interface different from the substrate. Small vesicles of 1-palmitoyl-2-oleoylglycero-sn-3-phosphoglycerol (POPG) or other anionic phospholipids are a neutral diluent interface for the partitioning of substrate and enzyme. TlL binds to these interfaces in an active or open form, thus implying a displacement of the helical lid away from the active site. A study of the influence of substrate and diluent concentration dependence of the rate of hydrolysis provides a basis for the determination of the primary interfacial catalytic parameters. The interfacial activation is not supported by zwitterionic vesicles or by large anionic vesicles of 100 nm diameter, although TlL binds to these interfaces. Using a combination of fluorescence-based techniques applied to several mutants of TlL with different tryptophan residues we have shown that TlL binds to phospholipid vesicles in different forms rendering different catalytic activities, and that the open lid conformation is achieved and stabilized by a combination of electrostatic and hydrophobic interactions between the enzyme's lipid-binding face and the interface.     

Effect of a phorbol ester on basic surface properties of trichomonads

The effect of nanomolar concentrations of 12-O-tetradecanoil-phorbol-13-acetate (TPA) on the cell surface of the urogenital parasitic protozoaTrichomonas vaginalis andTritrichomonas foetus was evaluated by means of measurements of the parasites’ surface tension, electrokinesis, lectin agglutination tests, and adhesion to inert substrates. TPA-treated parasites had their adhesion increased to both plastic and glass substrates. This was accompanied by increases in the parasites’ net negative surface charge and also by changes in their surface tension. The lectin agglutination assays suggest that the increase in surface negativeness may be related in some extent to alterations in the oligosaccharide composition. Successive treatment of the microorganisms with TPA and sphingosine, a well-known competitive inhibitor of the phorbol ester active site, depressed the tendency of trichomonads to exhibit a phenotype of activated cells.     

Effect of toroidal magnetic field variations on the spectra of azimuthal surface waves in metal waveguides entirely filled with plasma

The propagation of surface waves transverse to the circular axis of a toroidal magnetized metal waveguide entirely filled with a plasma is studied using perturbation theory. The distribution of the fields of these waves in such a waveguide structure is investigated. It is shown that the toroidicity introduces a second-order correction to the eigenfrequency of the surface waves.     

Effect of a phorbol ester on basic surface properties of trichomonads.

The effect of nanomolar concentrations of 12-O-tetradecanoilphorbol-13-acetate (TPA) on the cell surface of the urogenital parasitic protozoa Trichomonas vaginalis and Tritrichomonas foetus was evaluated by means of measurements of the parasites' surface tension, electrokinesis, lectin agglutination tests, and adhesion to inert substrates. TPA-treated parasites had their adhesion increased to both plastic and glass substrates. This was accompanied by increases in the parasites' net negative surface charge and also by changes in their surface tension. The lectin agglutination assays suggest that the increase in surface negativeness may be related in some extent to alterations in the oligosaccharide composition. Successive treatment of the microorganisms with TPA and sphingosine, a well-known competitive inhibitor of the phorbol ester active site, depressed the tendency of trichomonads to exhibit a phenotype of activated cells.     

Nonlinear theory of the excitation of azimuthal surface waves during dissipative instability

A theoretical study is made of the surface electromagnetic eigenmodes that are excited by an annular charged-particle beam due to dissipative instability and propagate across the external axial magnetic field in a cylindrical metal waveguide partially filled with plasma. A self-consistent set of differential equations for a cold low-density charged-particle beam moving above the plasma surface is constructed in the single-mode approximation and is solved numerically. It is shown that the larger the dissipation, the slower the instability growth rate and the larger the wave amplitude in the saturation stage of the instability. An increase in the transverse dimensions of a charged-particle beam results in a slower growth of the dissipative instability, in which case, however, the beam transfers a larger fraction of its kinetic energy to the wave.     

The optical cross section and absolute size of a photosynthetic unit

The concepts of a photosynthetic unit (PSU) and of an optical cross section are defined. The various estimates of sizes of photosynthetic units are described, and it is shown how an unambiguous measurement of the size of a unit can be obtained by measurement of its optical cross section via the saturation response to a single turnover light flash. The Emerson-Arnold unit must be divided by the quantum requirement for oxygen to obtain the true size of the unit. The size so obtained is the average number of chlorophylls per trap or reaction center. The effects of escape from open and closed traps are considered and it is shown that when these escape probabilities are equal, their effect on the saturation curve vanishes, leaving the simple cumulative one hit Poisson distribution.     

Investigation of the barrier discharge structure near the electrode with a cylindrical cross section

The approximate analytical correlations allowing for the investigation of the effect of different geometrical and physical parameters on the barrier discharge evolution near the electrode with a cylindrical cross section are obtained. The found correlations make it possible to estimate the time of the barrier discharge existence. New essential peculiarities of the barrier discharge evolution are revealed and verified.     

The surface properties of apolipoproteins A-I and A-II at the lipid/water interface

The monolayer system was employed to investigate the relative affinities of apolipoproteins A-I and A-II for the lipid/water interface. The adsorption of reductively 14C-methylated apolipoproteins to phospholipid monolayers spread at the air/water interface was determined by monitoring the surface pressure of the mixed monolayer and the surface concentration of the apoprotein. ApoA-II has a higher affinity than apoA-I for lipid monolayers; for a given initial surface pressure, apoA-II adsorbs more than apoA-I to monolayers of egg phosphatidylcholine (PC), distearoyl-PC and human high-density lipoprotein (HDL3) surface lipids. Comparison of the molecular packing of apolipoproteins A-I and A-II suggests that apoA-II adopts a more condensed conformation at the lipid/water interface compared to apoA-I. The ability of apoA-II to displace apoA-I from egg PC and HDL3 surface lipid monolayers was studied by following the adsorption and desorption of the reductively 14C-methylated apolipoproteins. At saturating subphase concentrations of the apoproteins (3.10(-5) g/100 ml), two molecules of apoA-II absorbed for each molecule of apoA-I displaced. This displacement was accompanied by an increase in surface pressure. An identical stoichiometry for the displacement of apoA-I from HDL particles by apoA-II has been reported by others. At low subphase concentrations of apoproteins (5.10(-6) g/100 ml), the apoA-I/lipid monolayer was not fully compressed and could accommodate the adsorbing apoA-II molecules without displacement of apoA-I molecules. ApoA-I molecules were unable to displace apoA-II from the lipid/water interface. The average residue hydrophobicity of apoA-II is higher than that of apoA-I; this may contribute to the higher affinity of apoA-II for lipids compared to apoA-I. The probable helical regions in apolipoproteins A-I and A-II were located using a secondary structure prediction algorithm. The analysis suggests that the amphiphilic properties of the alpha-helical regions of apoA-I and apoA-II are probably not significantly different. Further understanding of the differences in surface activity of these apolipoproteins will require more knowledge of their secondary and tertiary structures.     

Effect of bile on the lipid composition and surface properties of bifidobacteria

AIM: The changes produced on the bacterial surface of Bifidobacteria cells when they are grown in bile were compared with those provoked by bile added to bacteria grown in the absence of bile. METHODS AND RESULTS: The adhesive properties, the zeta potential and the lipid composition of Bifidobacterial strains, isolated from human faeces and grown in MRS medium, were determined. Bacteria grown in MRS with bile showed a loss of adherence and autoaggregation in correlation with a decrease in the surface hydrophobicity in comparison to those grown in MRS without bile, concomitant with the absence of two glycolipids, the increase of sugar content and minor changes in fatty acid composition. The surface changes caused by bile shock on bacteria grown in bile-free medium were much less pronounced and, in addition, no effect on the lipid composition was apparent. CONCLUSIONS: The comparison of the results indicates that bile action on surface properties is related to metabolic changes. SIGNIFICANCE AND IMPACT OF THE STUDY: Long-term exposure of bacteria to bile may cause metabolic changes affecting their adhesive properties irreversibly. This may be taken as a criterion to define the probiotic properties of different strains.     

Effect of the charge of the interface surface on the structure and activity of trypsin in reversed mycelles

The effect of the sign and value of the charge of the interface surface on the catalytic activity of trypsin in systems of reversed mycelles was investigated. n-Butanol was used for the modification of the phase interface in dispersions of reversed mycelles based on anionic sodium bis(2-ethylhexyl)sulfosuccinate (AOT) and cationic cetyltrimethylammonium bromide (CTAB). A direct correlation between changes in the state of reversed mycelles and the structure of solubilized enzyme under the action of butanol was obtained. It was shown that the enzyme activity is determined by the quantity of butanol solubilized by the reversed mycelles.     

Impact of photon cross section uncertainties on Monte Carlo-determined depth-dose distributions

This work studies the impact of systematic uncertainties associated to interaction cross sections on depth dose curves determined by Monte Carlo simulations. The corresponding sensitivity factors are quantified by changing cross sections by a given amount and determining the variation in the dose. The influence of total and partial photon cross sections is addressed. Partial cross sections for Compton and Rayleigh scattering, photo-electric effect, and pair production have been accounted for. The PENELOPE code was used in all simulations. It was found that photon cross section sensitivity factors depend on depth. In addition, they are positive and negative for depths below and above an equilibrium depth, respectively. At this depth, sensitivity factors are null. The equilibrium depths found in this work agree very well with the mean free path of the corresponding incident photon energy. Using the sensitivity factors reported here, it is possible to estimate the impact of photon cross section uncertainties on the uncertainty of Monte Carlo-determined depth dose curves.     

Effect of cross linkers on the bacteriorhodopsin photocycle

A general behavior of bacteriorhodopsin in purple membranes from Halobacterium halobium has been observed upon modification resulting in cross-linking of carboxyl and lysine groups. The rise of the M-intermediate contained two components with approximately 50-50% intensity; its decay showed three components with approximately 25-50-25% intensity respectively in a pH range of 5-9. The significance of these remarkably similar data with respect to the proton translocation mechanism in bacteriorhodopsin is that chemical modification allows us to conclude that disturbing parts of the hypothetical "proton conducting chain" does not inhibit proton translocation.     

Evaluating the genetic basis of multiple-locule fruit in a broad cross section of tomato cultivars

Lycopersicon esculentum accessions bearing fasciated (multiloculed) fruit were characterized based on their flower organ and locule number phenotypes. Greenhouse and field evaluations indicate that increases in locule number are associated with increases in the number of other floral organs (e.g., sepals, petals, stamens) in all stocks. F₁ complementation, F₂ segregation analysis, and genetic mapping indicate that at least four loci account for increases in the number of carpels/locules in these stocks. The most significant of these map to the bottoms of chromosomes 2 and 11 and correspond to the locule number and fasciated loci. All stocks tested were fixed for mutations at the fasciated locus, which maps to the 0.5-cM interval between the markers T302 and cLET24J2A and occurs in at least three allelic forms (wild type and two mutants). One of the fasciated mutant alleles is associated with nonfused carpels and repressed recombination and may be due to a small inversion or deletion. The other two loci controlling locule number correspond to the lcn1.1 and lcn2.2 loci located on chromosomes 1 and 2, respectively.