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1.
Guomin Huang Lan Hong Wanhui Ye Hao Shen Honglin Cao Wei Xiao 《Conservation Genetics》2009,10(4):1069-1071
Twelve novel microsatellite loci were isolated and characterized from enriched genomic libraries of Castanopsis chinensis. Four previously reported microsatellites from Castanopsis cuspidata were cross-amplified in C. chinensis. Forty-two sample trees from a wild population were tested for polymorphism using a set of the 16 polymorphic microsatellites.
The average allele number of these microsatellites was 4.6 per locus, ranging from 2 to 7. The ranges of observed and expected
heterozygosity were 0.262–1.000 and 0.238–0.818, respectively. Significant deviations from Hardy–Weinberg equilibrium were
detected at five loci and no linkage disequilibrium was observed. 相似文献
2.
Reeves’s Pheasant, Syrmaticus reevesii, is an endemic species of China. Due to habitat loss, poaching and human disturbance, its wild population has been decreased
drastically and it is listed as a globally vulnerable species by IUCN/BirdLife/WPA (IUCN 2008). Here, we report nine new polymorphic
microsatellite markers isolated from the Reeves’s Pheasant. The number of alleles per locus varies between four and fourteen, with expected heterozygosity ranging from 0.349–0.776 (n = 90). These polymorphic loci provide a valuable tool for future population studies that relate to the conservation of this
pheasant. 相似文献
3.
Junco Nagata Youichi Sonoda Keiko Hamaguchi Naoki Ohnishi Soh Kobayashi Ken Sugimura Fumio Yamada 《Conservation Genetics》2009,10(4):1121-1123
We report on the isolation and characterization of eight polymorphic and five monomorphic microsatellites in the Amami rabbit
(Pentalagus furnessi). Microsatellite polymorphism was determined using 25 individuals. There were 2–11 alleles for each polymorphic locus with
heterozygosity ranging between 0.08 and 0.76. Linkage disequilibrium was not suggested between any pairs among the eight polymorphic
loci. We suggest that these primers be used in future studies to monitor population size, determine dispersal patterns, and
genetic diversity within and between populations of this and related species. 相似文献
4.
Eight microsatellite loci were isolated from an enriched genomic library of the great leaf-nosed bat, Hipposideros armiger. The polymorphism of these loci was tested on a population of 48 individuals from Anhui Province, China. All loci revealed
the polymorphism ranging from three to 12 alleles. The observed heterozygosity values were from 0.213 to 0.875 and expected
heterozygosity from 0.232 to 0.820. No significant linkage disequilibrium was detected. Two loci significantly deviated from
Hardy–Weinberg equilibrium after Bonferroni correction. In addition, successful cross-amplification also suggested that these
microsatellite markers will facilitate research on the population genetics and gene flow of H. armiger and other related species. 相似文献
5.
Panyu Hua Tingting Guo Wenchao Liu Shuyi Zhang Stephen J. Rossiter 《Conservation Genetics》2009,10(3):597-600
We used the enriched genomic library method to isolate and characterize dinucleotide microsatellite loci in the least horseshoe
bat, Rhinolophus pusillus. Seventeen loci were obtained and tested on 31 individuals sampled from Guangxi Province in southern China. Thirteen of these
markers were polymorphic with expected heterozygosity ranging from 0.821 to 0.909. A total of 164 alleles were detected and
the number of alleles per locus ranged from 9 to 16 (mean 12.6). These polymorphic markers will be used to assess population
structure in R. pusillus. In addition, successful cross-amplification in five congeneric bat species suggests most of these markers will also be useful
for studying related species. 相似文献
6.
The finless porpoise, Neophocaena phocaenoides, is endemic to the coastal waters of the Indo-Pacific, ranging from the Persian Gulf to Japan. Nine tetranucleotide microsatellite
loci were isolated from the finless porpoise (Neophocaena phocaenoides). Polymorphism of each locus was assessed in 39 unrelated individuals from the Yellow Sea and the South China Sea of the
Chinese waters. The number of alleles per locus varied from 2 to 11. The ranges of observed and expected heterozygosity were
0.154–0.795 and 0.146–0.839, respectively. Cross-species amplification of these loci was tested in other cetacean species.
These microsatellite markers described here will be suitable for population genetic studies of finless porpoises and other
cetacean species. 相似文献
7.
María Calero-Riestra Fernando Alda Jose Antonio Dávila Jesús T. García 《European Journal of Wildlife Research》2012,58(1):379-383
New microsatellite loci for Tawny Pipit Anthus campestris were isolated from a genomic library. We were able to unambiguously score six loci: two were dinucleotide, one trinucleotide,
two tetranucleotide and one pentanucleotide that turned out to be sex-linked. Four out of six loci were polymorphic with 7–23
alleles in our population and an observed heterozygosity ranging between 0.286 and 0.936. Cross-utility of these markers was
tested in other 17 steppe-bird species of six families. In addition, 16 microsatellite loci developed for other species were
tested for cross-species amplification in A. campestris. Eight microsatellite markers were successfully amplified; seven of them were polymorphic with 2–43 alleles and an observed
heterozygosity of 0.040–0.863. Overall, 14 functional locus markers have been characterized for A. campestris that could be useful for future studies of paternity, genetic variability and population structure. 相似文献
8.
Fourteen polymorphic microsatellite loci were isolated and characterized from an AC-enriched genomic library of Liriodendron chinense (Magnoliaceae). The average allele number of these microsatellites was 5.2 per locus, ranging from three to eight. The observed
and expected heterozygosity at population level were 0.07–1.00 and 0.10–0.83, respectively. These sets of microsatellites
will be useful for studies of population genetic structure of L. chinense and L. tulipifera as well as to estimate fine-scale gene flow rates. 相似文献
9.
Eleven polymorphic microsatellite loci were isolated and characterized from an AC-enriched genomic library of Akebia trifoliate ssp. australis. The number of allele per locus ranged from 3 to 14. The observed heterozygosity and expected heterozygosity at population
level were 0.196–1.000 and 0.522–0.902, respectively. In addition, this set of microsatellites produced robust cross-species
amplification in other two related taxa, suggesting these microsatellite markers should provide a useful tool for genetic
and conservation studies of the Akebia species. 相似文献
10.
Patinopecten yessoensis is a kind of cold water shellfish, and is an important economic species in China. In our study, we developed and evaluated
simple sequence repeat (SSR) markers of P. yessoensis. Characteristics of 11 EST-SSR loci were investigated using 40 P. yessoensis individuals. The number of alleles per locus ranged from two to five. The observed heterozygosity ranged from 0.1026 to 0.9487, while
the expected heterozygosity ranged from 0.1865 to 0.7433. All loci except P4 departed from Hardy–Weinberg equilibrium (P < 0.05) significantly. There was no LD observed between all pairs of EST-SSRs loci. These loci and markers will be useful
for population genetics and systemic evolution of scallop. 相似文献
11.
The intermediate horseshoe bat (Rhinolophus affinis) is a widespread species in Southeast Asia. We developed 19 novel microsatellite loci from an enriched genomic library of
the bat, and tested their polymorphism using a single population from Guangdong province, southern China. The number of alleles
ranged from 3 to 15 per locus with the expected and observed heterozygosity ranging from 0.397 to 0.920 and 0.280–0.926, respectively.
Three markers significantly deviated from the Hardy-Weinberg expectations after Bonferroni correction and no linkage disequilibrium
was detected in any of loci. These microsatellite loci will be useful in studying the phylogeography of this species. 相似文献
12.
A total of 12 polymorphic microsatellite loci were developed and characterized for a Chinese medicinal plant, Epimedium brevicornum (Berberidaceae). A genomic DNA enrichment protocol was used to isolate microsatellite loci and polymorphism was explored
using 38 individuals from one natural population. The observed number of alleles ranged from 2–14. The ranges of observed
and expected heterozygosity were 0.00–0.83 and 0.15–0.88, respectively. In addition, successful cross-species amplification
of this set of microsatellite markers in other four medicinal Epimedium species suggested that they would provide a useful tool for the genetic and conservation studies of Epimedium species. 相似文献
13.
Yuan Huang Yong Li Xiao Hu Xue-Jun Ge Chang-Qin Zhang Chun-Lin Long 《Conservation Genetics》2009,10(3):515-517
A total of twelve polymorphic microsatellite loci were developed from polyploid endangered species, Omphalogramma vincaeflora (Primulaceae). These loci were screened for variability among 45 individuals from three populations in China. The primers
amplified loci with allele number ranging from 3 to 9, with an average of 4.25 per locus. Polymorphism information content
ranged from 0.23 to 0.86. Nei’s genetic diversity ranged from 0.34 to 0.86. These primers provide an opportunity to use polymorphic
DNA markers to study the population genetic structure and its breeding system in this species. 相似文献
14.
We report on the isolation and characterization of eight microsatellite markers in the Hainan Eld’s deer (Cervus eldi hainanus) from genomic DNA-enriched libraries. Thirty-three microsatellites were screened from the libraries, and 8 of the screened
microsatellites were polymorphic. The number of observed alleles for each locus in 47 individuals ranged from 2 to 9, and
the expected and observed heterozygosity was 0.141–0.792 and 0.128–0.957, respectively. Three loci (CEH-2, CEH-6 and CEH-8)
of eight deviated from Hardy-Weinberg expectation and no significant linkage association was found among all these loci. These
microsatellite markers provide useful tool for population genetic studies of the Eld’s deer. 相似文献
15.
Simple sequence repeats (SSRs) markers were developed for Betula maximowicziana using 2698 expressed sequence tags (ESTs) from the NCBI database. Out of 112 designed primer pairs, 54 showed clear PCR amplification
and 14 of these revealed polymorphism in eight individuals sampled across the species’ range. The number of alleles detected
and the expected heterozygosity ranged from 1 to 3 and 0.000 to 0.570, respectively, when these 14 loci were examined in 49
individuals from a single population. In the cross species transferability test, eight of the 14 loci were also polymorphic
in all four of the diploid, tetraploid and hexaploid Betula species examined. These results showed high transferability of the developed EST-SSRs and that these markers are likely to
be useful in studies of the population genetics of species in the genus Betula. 相似文献
16.
Andoniaina R. Andrianaivoarivelo Gary D. Shore Susie M. McGuire Richard K. B. Jenkins Olga Ramilijaona Edward E. Louis Jr. Rick A. Brenneman 《Conservation Genetics》2009,10(4):1025-1028
Twenty-two nuclear microsatellite loci were isolated from a genomic DNA library derived from Madagascar’s Rousettus madagascariensis. Marker characteristics were determined from a single population (37 individuals) from Fort Dauphin (southeastern Madagascar).
Sixteen of the 22 loci were within Hardy–Weinberg expectations. These loci are highly informative with polymorphic information
content values ranging between 0.757 and 0.916. These loci will provide valuable information for the study of population genetics
and gene flow within this species of bats. Due to the dramatic reduction and alteration of their habitat, data generated utilizing
this marker suite will potentially provide additional information for the effective long-term management of this near-threatened
bat species. 相似文献
17.
The habitat of Temminck’s Tragopan (Tragopan temminckii), a threatened species in China, has undergone severe fragmentation. Eight polymorphic microsatellite markers were isolated
from Temminck’s Tragopan. Polymorphism was studied using 24 individuals collected from the wild. All the loci were polymorphic
with number of alleles ranging from 6 to 21 and observed heterozygosity 0.38–0.83. These primers will be useful in studying
gene flow between patches of Temminck’s Tragopan habitat and the level of genetic diversity in isolated patches. 相似文献
18.
Five microsatellite loci of Helicoverpa armigera were isolated from a partial genomic library screened by oligonucleotide probes. Primers were designed to detect allelic variability and heterozygosity in 60 individuals collected from different host species. All loci were found to be polymorphic, have 8–11 alleles with expected heterozygosity ranging from 0.81 to 0.88. Our results indicate that the five microsatellite loci could provide valuable markers for population genetic and ecological studies of the cotton bollworm. 相似文献
19.
Iracilda Sampaio Maria Paula Cruz Schneider Horacio Schneider 《Primates; journal of primatology》1996,37(1):65-73
Wild populations ofAlouatta belzebul andA. seniculus from Brazil were surveyed in relation to 20 protein loci. Estimates of genetic variability demonstrated thatA. seniculus presents the highest level of heterozygosity among several New World monkey species studied for the same loci. Additional
information from DNA and chromosomes suggest thatA. seniculus andA. belzebul are not the closest species in theHershkovitz’sAlouatta seniculus group. 相似文献
20.
J.-M. Lacape D. Dessauw M. Rajab J.-L. Noyer B. Hau 《Molecular breeding : new strategies in plant improvement》2007,19(1):45-58
A series of 320 mapped simple sequence repeats (SSRs) have been used to screen the allelic diversity of tetraploid Gossypium species. Fourty-seven genotypes were analyzed representing (i) the wide spectrum of diversity of the cultivated pool and
of the primitive landraces of species G. hirsutum (‘marie-galante’, ‘punctatum’, ‘richmondi’, ‘morrilli’, ‘palmeri’, and ‘latifolium’, and ‘yucatanense’), and (ii) species
G. barbadense, G. darwinii and G. tomentosum. The polymorphism of 201 SSR loci revealed 1128 allelic variants ranging from 3 to 17 per locus. Neighbor-joining (NJ) method
based on genetic dissimilarities produced groupings consistent with the assignments of accessions both at species and at race
level. Our data confirmed the proximity of the Galapagos endemic species G. darwinii to species G. barbadense. Within species G. hirsutum, and as compared to the other 6 races, race yucatanense appeared as the most distant from cultivated genotypes. Race yucatanense
also exhibited the highest number of unique alleles. The important informative heterogeneity of the 201 SSR loci was exploited
to select the most polymorphic ones that were assembled into three series of genome-wide (i.e. each homoeologous AD chromosome
pair being equally represented) and mutliplexable (× 3) SSRs. Using one of these ‘genotyping set’, consisting of 39 SSRs (one
3-plex for each of the 13 AD chromosomes pairs) or 45 loci, we were able to assess the relationships between accessions and
the topology in the genetic diversity sampled. Such genotyping set of highly informative SSR markers assembled in PCR-multiplex,
while increasing genotyping throughput, will be applicable for molecular genetic diversity studies of large germplasm collections.
Electronic Supplementary Material Supplementary material is available in the online version of this article at and is accessible for authorized users. 相似文献