共查询到20条相似文献,搜索用时 15 毫秒
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Lianjun Sun Xiaojiao Li Yongcai Fu Zuofeng Zhu Lubin Tan Fengxia Liu Xianyou Sun Xuewen Sun Chuanqing Sun 《植物学报(英文版)》2013,55(10):938-949
Grain size is an important yield-related trait in rice. Intensive artificial selection for grain size during domestication is evidenced by the larger grains of most of today’s cultivars compared with t... 相似文献
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水稻的粒长是水稻粒型构成的因素之一,直接影响水稻的单产,而粒长又是稻米品质的重要指标之一。该研究利用选育含有稻瘟病抗性基因Pigm 1的长粒恢复系R20 4为研究材料,通过图位克隆的方法,对粒长基因进行鉴定,并对该长粒性状在育种中的应用进行了分析。结果显示:(1)该长粒为显性基因控制的性状。(2)将粒长基因GL12 1初步定位在水稻第12染色体上Indel 12 3和Indel 12 7之间,物理距离约4.5 Mb。(3)通过进一步开发标记,最终将该粒长基因GL12 1定位在标记Indel 10和Indel 16之间,物理距离约900 kb。(4)长粒恢复系R20 4与‘庆源A’、‘定源A’和‘启源A’测交组合的粒长均表现R20 4表型,而与‘靓香A’测交组合的粒长比R20 4更长。研究表明,粒长基因GL12 1为显性基因控制的性状,可能为一个新的粒长控制基因,该研究为后期GL12 1基因的克隆、功能研究以及粒型分子育种奠定了基础。 相似文献
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Genetic Analysis and Molecular Mapping of a Novel Gene Conferring Resistance to Rice Stripe Virus 总被引:1,自引:0,他引:1
Feng Zhao Zhijun Cai Tiezhu Hu Haigen Yao Li Wang Na Dong Bin Wang Zhengang Ru Wenxue Zhai 《Plant Molecular Biology Reporter》2010,28(3):512-518
Rice stripe virus (RSV) is one of the most damaging diseases affecting rice in East Asia. Rice variety 502 is highly resistant
to RSV, while variety 5112 is extremely susceptible. Field statistical data revealed that all “502 × 5112” F1 individuals were resistant to RSV and the ratio of resistant to susceptible plants was 3:1 in the F2 population and 1:1 in the BC1F1 population. These results indicated that a dominant gene, designated RSV1, controlled the resistance. Simple sequence repeat (SSR) analysis was subsequently carried out in an F2 population. Sixty SSR markers evenly distributed on the 12 rice chromosomes were screened and tested. Two markers, RM229
and RM206, showed linkage with RSV1. Based on this result, six SSR markers flanking RM229 and RM206 were further selected and tested. Results indicated that
SSR markers RM457 and RM473E were linked to RSV1 with a genetic distance of 4.5 and 5.0 cM, respectively. All of the four SSR markers (RM229, RM473E, RM457 and RM206) linked
to RSV1 were all located on chromosome 11, therefore RSV1 should be located on chromosome 11 also. In order to find some new markers more closely linked to the RSV1 gene, sequence-related amplified polymorphism (SRAP) analysis was performed. A total of 30 SRAP primer-pairs were analyzed,
and one marker SR1 showed linkage with RSV1 at a genetic distance of 2.9 cM. Finally, RSV1 gene was mapped on chromosome 11 between SSR markers RM457 and SRAP marker SR1 with a genetic distance of 4.5 cM and 2.9 cM,
respectively. 相似文献
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一个控制水稻籽粒长度主效基因--Lk-4(t)的BC2F2群体定位及其遗传效应分析 总被引:4,自引:0,他引:4
水稻籽粒大小和形状是影响稻米外观品质和产量的重要影响因素,对控制这些性状基因的定位和克隆有助于弄清籽粒大小基因的表达模式和相应的代谢系统,最终实现该性状的自由调控。运用SSR和CAPs标记对来源于蜀恢527//蜀恢527/小粒回交组合BC2F2群体800隐性长粒单株进行分析,定位了一个控制水稻籽粒长短的基因,Lk-4(t)。对F2和BC2F2群体籽粒大小形状和千粒重的遗传分析表明,回交能将大部分对目的基因效应具有干扰修饰作用的微效基因多态性除去,从而有利于对目的基因型的准确鉴定;在F2和BC2F2群体中只发现两类籽粒长短表现型,即短粒和长粒,并且二者分离比例符合3:1的典型一对等位基因分离比例。这说明群体中籽粒长短变异是受一对基因控制。通过对BC2F2群体中隐性(长粒)单株进行分子标记分析,将这个控制籽粒长短的主效基因定位在3个CAPs标记,P1-EcoRV,P2-SacⅠ和P3-MboⅠ附近。连锁分析表明,Lk-4(t)位于水稻第3染色体着丝粒附近,离标记P1-EcoRⅤ和P2-SacⅠ分别有0.90cM和0.50cM的距离。 相似文献
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水稻产量和稻米品质的提高是水稻研究的中心问题。水稻产量主要取决于单株穗数、每穗粒数和粒重;粒重作为一个非常重要的产量性状,由粒长、粒宽和粒厚所决定。影响粒重和粒形的基因多为数量性状基因,精细定位并克隆到的较少。本研究中,我们克隆到一个影响粒形的基因SL,超表达(SL-OE)转基因植株表现出粒长增加、粒宽减小、叶宽减小的表型;同时,SL-RNAi的转基因植株呈现出粒长缩短、叶宽增加的表型。颖壳表面细胞在超表达转基因植株中伸长,而在RNAi转基因植株中缩短。叶片横向细胞数目在转基因植株中发生变化,推测乩基因可能与细胞分裂相关。SL-OE转基因植株中G嗽因被明显上调,说明盟基因可能通过调节GW2的表达对水稻粒宽造成影响。另外,观基因影响稻米的品质。 相似文献
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水稻糙米高蛋白基因的QTL定位 总被引:5,自引:1,他引:5
利用由糙米蛋白含量高达14.55%的广东省农家品种三春种配制的BC1群体进行糙米高蛋白基因的QTL定位,定位到了6个糙米蛋白基因的QTLs,其中有3个新的QTLs。有1个增效基因qCP1-2为主效基因,解释的表型变异高达44.2%,有可能是与谷蛋白基因Glu-1紧密连锁的新调控序列。本研究表明,所利用的BC1群体的糙米蛋白可能主要是由1个主效QTL所控制。 相似文献
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Analysis of the Molecular Evolutionary History of the Ascorbate Peroxidase Gene Family: Inferences from the Rice Genome 总被引:3,自引:0,他引:3
Teixeira FK Menezes-Benavente L Margis R Margis-Pinheiro M 《Journal of molecular evolution》2004,59(6):761-770
Ascorbate peroxidase (APx) is a class I peroxidase that catalyzes the conversion of H2O2 to H2O and O2 using ascorbate as the specific electron donor. This enzyme has a key function in scavenging reactive oxygen species (ROS) and the protection against toxic effects of ROS in higher plants, algae, and Euglena. Here we report the identification of an APx multigene family in rice and propose a molecular evolutionary relationship between the diverse APx isoforms. In rice, the APx gene family has eight members, which encode two cytosolic, two putative peroxisomal, and four chloroplastic isoforms, respectively. Phylogenetic analyses were conducted using all APx protein sequences available in the NCBI databases. The results indicate that the different APx isoforms arose by a complex evolutionary process involving several gene duplications. The structural organization of APx genes also reflects this process and provides evidence for a close relationship among proteins located in the same subcellular compartment. A molecular evolutionary pathway, in which cytosolic and peroxisomal isoforms diverged early from chloroplastic ones, is proposed.Reviewing Editor: Dr. Niles Lehman 相似文献
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A Germin-Like Protein Gene Family Functions as a Complex Quantitative Trait Locus Conferring Broad-Spectrum Disease Resistance in Rice 总被引:2,自引:0,他引:2
Patricia M. Manosalva Rebecca M. Davidson Bin Liu Xiaoyuan Zhu Scot H. Hulbert Hei Leung Jan E. Leach 《Plant physiology》2009,149(1):286-296
Plant disease resistance governed by quantitative trait loci (QTL) is predicted to be effective against a broad spectrum of pathogens and long lasting. Use of these QTL to improve crop species, however, is hindered because the genes contributing to the trait are not known. Five disease resistance QTL that colocalized with defense response genes were accumulated by marker-aided selection to develop blast-resistant varieties. One advanced backcross line carrying the major-effect QTL on chromosome (chr) 8, which included a cluster of 12 germin-like protein (OsGLP) gene members, exhibited resistance to rice (Oryza sativa) blast disease over 14 cropping seasons. To determine if OsGLP members contribute to resistance and if the resistance was broad spectrum, a highly conserved portion of the OsGLP coding region was used as an RNA interference trigger to silence a few to all expressed chr 8 OsGLP family members. Challenge with two different fungal pathogens (causal agents of rice blast and sheath blight diseases) revealed that as more chr 8 OsGLP genes were suppressed, disease susceptibility of the plants increased. Of the 12 chr 8 OsGLPs, one clustered subfamily (OsGER4) contributed most to resistance. The similarities of sequence, gene organization, and roles in disease resistance of GLP family members in rice and other cereals, including barley (Hordeum vulgare) and wheat (Triticum aestivum), suggest that resistance contributed by the chr 8 OsGLP is a broad-spectrum, basal mechanism conserved among the Gramineae. Natural selection may have preserved a whole gene family to provide a stepwise, flexible defense response to pathogen invasion. 相似文献
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一个新的水稻小粒矮秆基因的分子标记定位及效应分析 总被引:6,自引:0,他引:6
从水稻(Oryza safjva L.)半矮秆品种蜀恢I62中发现一份小粒矮秆突变体“I62d”。对I62d与4个半矮秆品种杂交F1和F2代的遗传分析表明,I62d的矮生性由一对隐性基因控制。以II-32B/162d F2代作定位群体,用分子标记将I62d突变基凶定位丁水稻第3染色体短臂,该基因与微卫星标记RM218和RMI57之间的遗传距离分别为3.5cM和10.0cM。同时,利用近等基因系分析了该基因的表型效应,结果表明它可使株高降为正常高度的1/4左右,籽粒降为正常大小的1/4左右,并使叶片显著缩短、加宽,结实率显著降低。我们认为162d突变基因是一个新的水稻小粒矮秆某因,暂命名为dI62(t)。 相似文献
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Evolutionary History of the Sex-Peptide (Acp70a) Gene Region in Drosophila Melanogaster 总被引:1,自引:0,他引:1 下载免费PDF全文
In Drosophila the products of the seminal fluid stimulate oviposition and suppress remating in the female. Of all the accessory gland peptides (Acp's) involved in these two responses, the sex-peptide (coded by the Acp70A gene) is among the best characterized at the functional level. A 1.2-kb fragment encompassing the Acp70A gene of nine lines from a natural population of D. melanogaster and one allele of D. sechellia was sequenced to study the forces shaping nucleotide variation within and between species. The coding region of D. simulans and D. mauritiana was also sequenced. A Ser to Ala replacement polymorphism at the last position of the signal peptide was detected in D. melanogaster. The Ser and Ala alleles are at intermediate frequencies. The level of nucleotide variation is lower for the derived Ala allele, which is compatible with a recent origin and an increase in frequency due to positive selection. Variation at the 5' flanking region is structured in two major highly differentiated haplotypes, whose distribution does not conform to neutral expectations. Selective and/or historical factors could contribute to the observed overall patterning of nucleotide variation at the Acp70A region. 相似文献
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一个新的水稻小粒矮秆基因的分子标记定位及效应分析
李秀兰 吴 成 邓晓建 王平荣 李仁端 杨志荣 相似文献
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Members of cytochrome P450 subfamily 1A (CYP1As) are involved in detoxification and bioactivation of common environmental
pollutants. Understanding the functional evolution of these genes is essential to predicting and interpreting species differences
in sensitivity to toxicity caused by such chemicals. The CYP1A gene subfamily comprises a single ancestral representative
in most fish species and two paralogs in higher vertebrates, including birds and mammals. Phylogenetic analysis of complete
coding sequences suggests that mammalian and bird paralog pairs (CYP1A1/2 and CYP1A4/5, respectively) are the result of independent
gene duplication events. However, comparison of vertebrate genome sequences revealed that CYP1A genes lie within an extended
region of conserved fine-scale synteny, suggesting that avian and mammalian CYP1A paralogs share a common genomic history.
Algorithms designed to detect recombination between nucleotide sequences indicate that gene conversion has homogenized most
of the length of the chicken CYP1A genes, as well as the 5′ end of mammalian CYP1As. Together, these data indicate that avian
and mammalian CYP1A paralog pairs resulted from a single gene duplication event and that extensive gene conversion is responsible
for the exceptionally high degree of sequence similarity between CYP1A4 and CYP1A5. Elevated nonsynonymous/synonymous substitution
ratios within a putatively unconverted stretch of ∼250 bp suggests that positive selection may have reduced the effective
rate of gene conversion in this region, which contains two substrate recognition sites. This work significantly alters our
understanding of functional evolution in the CYP1A subfamily, suggesting that gene conversion and positive selection have
been the dominant processes of sequence evolution.
Electronic Supplementary Material Electronic Supplementary material is available for this article at
and accessible for authorised users.
[Reviewing Editor: Dr. Yves Van de Peer] 相似文献
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Grain length is an important quantitative trait in rice (Oryza sativa L.) that influences both grain yield and exterior quality. Although many quantitative trait loci (QTLs) for grain length have been identified, it is still unclear how different alleles from different QTLs regulate grain length coordinately. To explore the mechanisms of QTL combination in the determination of grain length, five mapping populations, including two F2 populations, an F3 population, an F7 recombinant inbred line (RIL) population, and an F8 RIL population, were developed from the cross between the U.S. tropical japonica variety ‘Lemont’ and the Chinese indica variety ‘Yangdao 4’ and grown under different environmental conditions. Four QTLs (qGL-3-1, qGL-3-2, qGL-4, and qGL-7) for grain length were detected using both composite interval mapping and multiple interval mapping methods in the mapping populations. In each locus, there was an allele from one parent that increased grain length and another allele from another parent that decreased it. The eight alleles in the four QTLs were analyzed to determine whether these alleles act additively across loci, and lead to a linear relationship between the predicted breeding value of QTLs and phenotype. Linear regression analysis suggested that the combination of eight alleles determined grain length. Plants carrying more grain length-increasing alleles had longer grain length than those carrying more grain length-decreasing alleles. This trend was consistent in all five mapping populations and demonstrated the regulation of grain length by the four QTLs. Thus, these QTLs are ideal resources for modifying grain length in rice. 相似文献
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Jean-Luc Desseyn Marie-Pierre Buisine Nicole Porchet Jean-Pierre Aubert Pierre Degand Anne Laine 《Journal of molecular evolution》1998,46(1):102-106
The four human mucin genes MUC6, MUC2, MUC5AC, and MUC5B are located at chromosome 11p15.5. It has been demonstrated that the three mucins MUC2, MUC5AC, and MUC5B contain several
Cys-subdomains of 108 amino acid residues. In contrast, little information is available concerning MUC6. These Cys-subdomains
contain 10 cysteine residues that have a highly conserved position. We present here a coherent probable evolutionary history
of this human gene family after comparison of the nucleotide sequences of these Cys-subdomains. The three MUC loci MUC2, MUC5AC, and MUC5B may have evolved from a common ancestral gene by two successive duplications. Moreover, we can postulate that MUC5AC and MUC5B have evolved in a concerted manner, while MUC2 has evolved separately.
Received: 30 January 1997 / Accepted: 17 April 1997 相似文献
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选取竹亚科中两个超族、六个族和三个亚族的10个竹种为材料,分别是泰竹、凤尾竹、青皮竹、大叶慈、慈竹、野龙竹、毛竹、香竹、苦竹、菲白竹,分离克隆了它们的lea3基因,并将它们与外类群物种水稻进行序列比对和进化分析。结果发现在分支模型与分支位点模型的检测中,不同竹种所含lea3基因承受了不同的正选择压力,清除选择作用在lea3基因编码区中占主导地位(ω<1)。在位点模型的检测中,共检测出了18个显著性正选择位点,占总氨基酸数目的111%。对这18个显著性正选择位点进行定位后,发现其中的15个位于11个氨基酸串联重复序列附近。这说明lea3基因中的11个氨基酸串联重复序列区比基因其它区域更容易受自然选择作用影响。同时,在位点模型检测结果的基础上,通过对强烈清除选择位点的定位,发现在11个氨基酸串联重复序列区内存在一长段无强烈清除位点的序列区。 相似文献