MTR1 encodes a secretory fasciclin glycoprotein required for male reproductive development in rice

1. Download : Download high-res image (356KB)
2. Download : Download full-size image

Highlights? The fasciclin glycoprotein MTR1 regulates male reproductive development in rice ? Rice male reproductive cells secrete MTR1 to control somatic tapetum development     

Structural and functional analysis of HtrA1 and its subdomains

1. Download : Download high-res image (216KB)
2. Download : Download full-size image

Highlights? IGFBP-like domain structure suggests that it is incompetent for IGF binding ? Kazal-like domain structure shows distorted “P1” loop incompetent for inhibition ? Protease domain shows catalytic readiness in apo form ? SAXS envelope for full-length HtrA1     

A Cell Cycle-Dependent Regulatory Circuit Composed of 53BP1-RIF1 and BRCA1-CtIP Controls DNA Repair Pathway Choice

1. Download : Download high-res image (124KB)
2. Download : Download full-size image

Highlights? 53BP1 inhibits BRCA1 recruitment to DSB sites in G1 ? RIF1 is the effector of 53BP1 during DSB repair ? Class-switch recombination requires RIF1 ? RIF1 recruitment to DSB sites in S/G2 is inhibited by BRCA1-CtIP     

Metabolic Stress Controls mTORC1 Lysosomal Localization and Dimerization by Regulating the TTT-RUVBL1/2 Complex

1. Download : Download high-res image (230KB)
2. Download : Download full-size image

Highlights? Glucose and glutamine feed back to promote mTORC1 signaling through ATP production ? Energetic stress prevents mTOR lysosomal localization independently of AMPK and Rag ? ATP-dependent TTT-RUVBL complex is disassembled and repressed by energetic stress ? TTT-RUVBL is required for mTORC1 functional assembly and lysosomal localization     

GRSF1 Regulates RNA Processing in Mitochondrial RNA Granules

1. Download : Download high-res image (237KB)
2. Download : Download full-size image

Highlights? GRSF1 resides in the mitochondrial matrix and is required for mitochondrial function ? GRSF1 is required for the processing of tRNA-containing and tRNA-lacking precursors ? GRSF1, RNase P, and nascent RNA are part of “mitochondrial RNA granules” ? Mitochondrial RNA granules are functionally linked to RNA processing     

Dynamin 2 Potentiates Invasive Migration of Pancreatic Tumor Cells through Stabilization of the Rac1 GEF Vav1

1. Download : Download high-res image (180KB)
2. Download : Download full-size image

Highlights? Dyn2 promotes lamellipodia formation and pancreatic tumor cell migration ? Direct binding between Dyn2 and Vav1 promotes Rac1 activation and migration ? Dyn2 binding protects Vav1 from degradation by the lysosome ? Vav1 is targeted for degradation through an interaction with Hsc70     

POT1-TPP1 Regulates Telomeric Overhang Structural Dynamics

1. Download : Download high-res image (114KB)
2. Download : Download full-size image

Highlights? POT1 binds the telomere overhang sequentially one OB fold at a time ? POT1 binding exhibits 3′ to 5′ directionality ? POT1-TPP1N induces dynamic folding and unfolding of telomeric overhang ? POT1-TPP1N slides back and forth on the telomeric overhang     

A DNA-Damage Selective Role for BRCA1 E3 Ligase in Claspin Ubiquitylation,CHK1 Activation,and DNA Repair

1. Download : Download high-res image (201KB)
2. Download : Download full-size image

Highlights? BRCA1 E3 ligase activity is essential for a subset of DNA damage responses ? BRCA1 V26A mutant cells are sensitive to topoisomerase inhibitors but not mitomycin C ? BRCA1 selectively ubiquitylates claspin after topoisomerase inhibition ? BRCA1 E3 ligase inactivation selectively impairs CHK1 activation     

MT1-MMP Is Required for Myeloid Cell Fusion via Regulation of Rac1 Signaling

1. Download : Download high-res image (231KB)
2. Download : Download full-size image

Highlights? Myeloid cells fuse during osteoclast and giant cell formation ? MT1-MMP is required for this fusion in vitro and in vivo ? MT1-MMP regulates the GTPase Rac1 through p130Cas binding ? MT1-MMP proteolytic activity is not required     

PGRL1 Is the Elusive Ferredoxin-Plastoquinone Reductase in Photosynthetic Cyclic Electron Flow

1. Download : Download high-res image (156KB)
2. Download : Download full-size image

Highlights? PGRL1 is the elusive FQR which has been sought for almost three decades ? PGR5 is required for the transfer of electrons from ferredoxin to PGRL1 ? The six redox-active cysteine residues of PGRL1 can form intra- and intermolecular disulfide bridges ? Thioredoxins destabilize PGRL1 homodimers     

SIP1 Mediates Cell-Fate Decisions between Neuroectoderm and Mesendoderm in Human Pluripotent Stem Cells

1. Download : Download high-res image (184KB)
2. Download : Download full-size image

Highlights? Smad-interacting protein 1 (SIP1) regulates hESC differentiation ? SIP1 upregulation promotes neuroectodermal differentiation ? SIP1 inhibits mesendodermal and endodermal differentiation ? SMAD2/3 and NANOG/OCT4/SOX2 cooperatively regulate SIP1 expression     

Mechanism for KRIT1 Release of ICAP1-Mediated Suppression of Integrin Activation

1. Download : Download high-res image (110KB)
2. Download : Download full-size image

Highlights? Cocrystal structure of KRIT1 with ICAP1 shows a bidentate interaction interface ? Cocrystal structure of ICAP1 with integrin β1 shows a PTB-peptide interaction ? KRIT1 antagonizes ICAP1-modulated integrin activation by the bidentate interaction ? KRIT1 contains a previously undescribed N-terminal Nudix domain     

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain

1. Download : Download high-res image (337KB)
2. Download : Download full-size image

Highlights? ESCRT-I subunit UBAP1 is essential for degradation of antiviral protein tetherin ? UBAP1 has a domain consisting of a solenoid of overlapping UBAs (SOUBA) ? Each of the three UBAs in the SOUBA binds monoubiquitin     

The Microtubule-Binding Protein Ensconsin Is an Essential Cofactor of Kinesin-1

1. Download : Download high-res image (155KB)
2. Download : Download full-size image

Highlights? Ensconsin is required for cargo transport driven by kinesin-1 ? The C terminus of ensconsin activates kinesin-1; its N terminus binds microtubules ? Kinesin-1 mutants without autoinhibitory activity do not require ensconsin     

Keeping cell identity in Arabidopsis requires PRC1 RING-finger homologs that catalyze H2A monoubiquitination

1. Download : Download high-res image (139KB)
2. Download : Download full-size image

Highlights? AtBMI1A/B proteins prevent misexpression of embryonic traits in somatic cells ? PRC2 and AtBMI1A/B proteins maintain cells in their differentiated state ? AtBMI1A/B proteins mediate H2A monoubiquitination     

The Retroviral Restriction Ability of SAMHD1, but Not Its Deoxynucleotide Triphosphohydrolase Activity,Is Regulated by Phosphorylation

1. Download : Download high-res image (130KB)
2. Download : Download full-size image

Highlights? SAMHD1 only exhibits antiviral activity when expressed in noncycling cells ? SAMHD1 is unphosphorylated in noncycling cells ? Reverse genetics revealed that phosphorylated SAMHD1 is repressive for restriction ? SAMHD1 phosphorylation regulates restriction, but not the cellular levels of dNTPs     

RIF1 Is Essential for 53BP1-Dependent Nonhomologous End Joining and Suppression of DNA Double-Strand Break Resection

1. Download : Download high-res image (298KB)
2. Download : Download full-size image

Highlights? RIF1 is essential for 53BP1-dependent CSR and fusion of dysfunctional telomeres ? BRCA1 antagonizes RIF1 in S phase to prevent error-prone repair by toxic NHEJ ? N-terminal phospho-SQ/TQ domain of 53BP1 interacts with and recruits RIF1 to DSBs ? RIF1 and 53BP1 promote NHEJ in G1 by blocking 5′ end resection of DSBs     

Redox-Dependent Control of FOXO/DAF-16 by Transportin-1

1. Download : Download high-res image (206KB)
2. Download : Download full-size image

Highlights? FOXO forms redox-sensitive, disulfide-dependent complexes with several proteins ? Transportin-1 binds to FOXO via a disulfide and regulates its nuclear localization ? Redox and insulin signaling govern FOXO nuclear localization via distinct pathways ? Redox control of longevity protein FOXO/DAF-16 is evolutionarily conserved     

PGC-1 Coactivators Regulate MITF and the Tanning Response

1. Download : Download high-res image (175KB)
2. Download : Download full-size image

Highlights? PGC-1 induces pigment formation in melanocytes ? PGC-1s activate expression of MITF ? α-MSH induces PGC-1s, which are required for induction of melanogenic genes ? eQTLs in human PGC-1β are associated with tanning ability and melanoma protection     

Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression

1. Download : Download high-res image (218KB)
2. Download : Download full-size image

Highlights? Rac1 and the Scar/WAVE complex drive pseudopod-based motility of melanoblasts ? Rac1-depleted melanoblasts move using unique actin-based stubs and not blebs ? Rac1 controls pseudopod frequency but is dispensable for pseudopod formation ? Loss of Rac1 delays melanoblast cell-cycle progression and cytokinesis